r3140 (New England Biolabs)


Name:
PstI HF
Description:
PstI HF 50 000 units
Catalog Number:
r3140l
Price:
269
Category:
Restriction Enzymes
Size:
50 000 units
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PstI HF 50 000 units
https://www.bioz.com/result/r3140/product/New England Biolabs
Average 94 stars, based on 1 article reviews
Price from $9.99 to $1999.99
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Southern Blot:Article Title: Endogenous viral element-derived piRNAs are not required for production of ping-pong-dependent piRNAs from Diaphorina citri densovirus Article Snippet: PCR products were purified with the Zymoclean Gel DNA Recovery Kit (Zymo Research, Irvine, California) and sequenced by Quintarabio using the Sanger method. .. Southern blotting 5 µg undigested CRF-CA D citri DNA or 5 µg CRF-CA D. citri DNA digested overnight with Incubation:Article Title: Endogenous viral element-derived piRNAs are not required for production of ping-pong-dependent piRNAs from Diaphorina citri densovirus Article Snippet: PCR products were purified with the Zymoclean Gel DNA Recovery Kit (Zymo Research, Irvine, California) and sequenced by Quintarabio using the Sanger method. .. Southern blotting 5 µg undigested CRF-CA D citri DNA or 5 µg CRF-CA D. citri DNA digested overnight with Purification:Article Title: Mobius Assembly: A versatile Golden-Gate framework towards universal DNA assembly Article Snippet: They were then amplified using Q5 DNA polymerase (NEB) with primers bearing EcoRI , AarI , BsaI , and PstI restriction sites and 4 bp overhangs. .. Subsequently the pSB1C3/pSB1K3 backbones and the Golden Gate cassettes were digested with EcoRI-HF and Polymerase Chain Reaction:Article Title: Mobius Assembly: A versatile Golden-Gate framework towards universal DNA assembly Article Snippet: They were then amplified using Q5 DNA polymerase (NEB) with primers bearing EcoRI , AarI , BsaI , and PstI restriction sites and 4 bp overhangs. .. Subsequently the pSB1C3/pSB1K3 backbones and the Golden Gate cassettes were digested with EcoRI-HF and Clone Assay:Article Title: Stable integration of an optimized inducible promoter system enables spatiotemporal control of gene expression throughout avian development Article Snippet: The cDNA synthesis reaction was carried out at 50°C for 30 min, 55°C for 10 min, 60°C for 10 min, 65°C for 10 min, and 85°C for 5 min. Full length Runx2 , Mmp13 , Cxcl14 , and Gas1 were amplified by PCR using Q5 Hot Start High-Fidelity DNA Polymerase (NEB, Ipswich, MA, USA, M0493L) and cloned using CloneJET PCR Cloning Kit (Thermo Fisher Scientific, Waltham, MA, USA, K1231). .. Following confirmation of cloning of full length coding sequences by Sanger sequencing, Runx2 , Mmp13 , Cxcl14 , and Gas1 were cloned into pEPIC1.1 digested with AflII (NEB, Ipswich, MA, USA, R0520S) and EcoRI (NEB, Ipswich, MA, USA, R3101S) or pPIDNB digested with AflII (NEB, Ipswich, MA, USA, R0520S) and Sequencing:Article Title: Stable integration of an optimized inducible promoter system enables spatiotemporal control of gene expression throughout avian development Article Snippet: The cDNA synthesis reaction was carried out at 50°C for 30 min, 55°C for 10 min, 60°C for 10 min, 65°C for 10 min, and 85°C for 5 min. Full length Runx2 , Mmp13 , Cxcl14 , and Gas1 were amplified by PCR using Q5 Hot Start High-Fidelity DNA Polymerase (NEB, Ipswich, MA, USA, M0493L) and cloned using CloneJET PCR Cloning Kit (Thermo Fisher Scientific, Waltham, MA, USA, K1231). .. Following confirmation of cloning of full length coding sequences by Sanger sequencing, Runx2 , Mmp13 , Cxcl14 , and Gas1 were cloned into pEPIC1.1 digested with AflII (NEB, Ipswich, MA, USA, R0520S) and EcoRI (NEB, Ipswich, MA, USA, R3101S) or pPIDNB digested with AflII (NEB, Ipswich, MA, USA, R0520S) and Electrophoresis:Article Title: Kaposi's Sarcoma-Associated Herpesvirus ORF68 Is a DNA Binding Protein Required for Viral Genome Cleavage and Packaging Article Snippet: DNA was isolated by phenol-chloroform extraction and ethanol precipitation and then resuspended in TE buffer. .. DNA (10 μg) was digested with Staining:Article Title: Kaposi's Sarcoma-Associated Herpesvirus ORF68 Is a DNA Binding Protein Required for Viral Genome Cleavage and Packaging Article Snippet: DNA was isolated by phenol-chloroform extraction and ethanol precipitation and then resuspended in TE buffer. .. DNA (10 μg) was digested with Plasmid Preparation:Article Title: Methylase-assisted subcloning for high throughput BioBrick assembly Article Snippet: The purified restriction fragments were ligated (20 fmol ∼60 ng tagRFP-pUC, 90 ng lacI-Ptac-lacO + pUC, 50 nM PstI “unlinker”) in temperature cycled NEB T4 DNA ligase buffer (20 µL total reaction volume) prior to heat killing and transformation of E. coli as described above. .. 3A assemblyA BioBrick-compatible plasmid that encodes chloramphenicol acetyltransferase, RP4 oriT-pUC57-mini-cat (2 µg) in 1× NEB CutSmart buffer (80 µL total reaction volume) by 20 units each of EcoRI-HF, |