r3140 (New England Biolabs)
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Name:
PstI HF
Description:
PstI HF 50 000 units
Catalog Number:
R3140L
Price:
264
Size:
50 000 units
Category:
Restriction Enzymes
Score:
85
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Structured Review
New England Biolabs
r3140
PstI HF 50 000 units
https://www.bioz.com/result/r3140/product/New England Biolabs
Average 97 stars, based on 1 article reviews
Price from $9.99 to $1999.99
PstI HF 50 000 units
https://www.bioz.com/result/r3140/product/New England Biolabs
Average 97 stars, based on 1 article reviews
Price from $9.99 to $1999.99
r3140 - by Bioz Stars,
2019-10
97/100 stars
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Clone Assay:Article Title: Mobius Assembly: A versatile Golden-Gate framework towards universal DNA assembly Article Snippet: All the standard parts were domesticated for Aar I and Bsa I when necessary and were cloned into mUAV. .. Subsequently the pSB1C3/pSB1K3 backbones and the Golden Gate cassettes were digested with EcoRI-HF and Article Title: PsrA controls the synthesis of the Pseudomonas aeruginosa quinolone signal via repression of the FadE homolog, PA0506 Article Snippet: Strain PAO1 chromosomal DNA was utilized as template for the PCR. .. The final PCR product was cloned into pEX18Ap using Amplification:Article Title: Mobius Assembly: A versatile Golden-Gate framework towards universal DNA assembly Article Snippet: They were then amplified using Q5 DNA polymerase (NEB) with primers bearing EcoRI , AarI , BsaI , and PstI restriction sites and 4 bp overhangs. .. Subsequently the pSB1C3/pSB1K3 backbones and the Golden Gate cassettes were digested with EcoRI-HF and Article Title: The Pattern and Distribution of Induced Mutations in J. curcas Using Reduced Representation Sequencing Article Snippet: 200 ng of genomic DNA were digested with 10 U Article Title: Special features of RAD Sequencing data: implications for genotyping Article Snippet: PstI-digested RAD libraries were prepared following the study by Baird et al . , using 20 units of Article Title: Assessing genotype-phenotype associations in three dorsal colour morphs in the meadow spittlebug Philaenus spumarius (L.) (Hemiptera: Aphrophoridae) using genomic and transcriptomic resources Article Snippet: Three RAD libraries with twelve individuals each were prepared following a modified RAD sequencing protocol [ ], using Article Title: Multispecies Outcomes of Sympatric Speciation after Admixture with the Source Population in Two Radiations of Nicaraguan Crater Lake Cichlids Article Snippet: Briefly, for each individual 600 ng DNA template were digested with the Article Title: The Pattern and Distribution of Induced Mutations in J. curcas Using Reduced Representation Sequencing Article Snippet: 200 ng of genomic DNA were digested with 10 U Article Title: Efficiency of multi-trait, indirect, and trait-assisted genomic selection for improvement of biomass sorghum Article Snippet: Illumina libraries were created using two pairs of restriction enzymes: PstI-HF/HinP1I and Construct:Article Title: Construction of a high-density linkage map and fine mapping of QTL for growth in Asian seabass Article Snippet: RAD libraries were constructed using the double digest RADseq method with some modifications . .. 200 ng of DNA from each sample was digested with 20 units of Article Title: Mobius Assembly: A versatile Golden-Gate framework towards universal DNA assembly Article Snippet: Subsequently the pSB1C3/pSB1K3 backbones and the Golden Gate cassettes were digested with EcoRI-HF and Article Title: PsrA controls the synthesis of the Pseudomonas aeruginosa quinolone signal via repression of the FadE homolog, PA0506 Article Snippet: The alleles were constructed to harbor in-frame deletions in the DNA coding sequence corresponding to amino acids 18 to 226 for psrA (89% of protein sequence), 19 to 585 for PA0506 (94% of protein sequence), 36 to 553 for PA0507 (86% of protein sequence), and 38 to 535 for PA0508 (86% of protein sequence). .. The final PCR product was cloned into pEX18Ap using Real-time Polymerase Chain Reaction:Article Title: Fine mapping QTL for resistance to VNN disease using a high-density linkage map in Asian seabass Article Snippet: In brief, 200 ng of each DNA was digested with 20 units of restriction enzyme of Incubation:Article Title: The Pattern and Distribution of Induced Mutations in J. curcas Using Reduced Representation Sequencing Article Snippet: 200 ng of genomic DNA were digested with 10 U Article Title: Downstream promoter interactions of TFIID TAFs facilitate transcription reinitiation Article Snippet: Transcription activator Gal4-vp16 (800 ng) was incubated with templates for 5 min at room temperature. .. Downstream DNA and bound proteins were released with 120 U of SspI-HF or Article Title: The Pattern and Distribution of Induced Mutations in J. curcas Using Reduced Representation Sequencing Article Snippet: 200 ng of genomic DNA were digested with 10 U Article Title: Analysis of genetic polymorphisms and tropism in East African Leishmania donovani by Amplified Fragment Length Polymorphism and kDNA minicircle sequencing Article Snippet: AFLP was carried out as previously described ( ) with some modifications. .. Following digestion with TaqI (10 U, New England Biolabs®), the mixture was cooled on ice, and then Mass Spectrometry:Article Title: Downstream promoter interactions of TFIID TAFs facilitate transcription reinitiation Article Snippet: Paragraph title: Immobilized template binding and quantitative mass spectrometry ... Downstream DNA and bound proteins were released with 120 U of SspI-HF or Modification:Article Title: PsrA controls the synthesis of the Pseudomonas aeruginosa quinolone signal via repression of the FadE homolog, PA0506 Article Snippet: Mutant P . aeruginosa strains were derived by a modified version of a previously published protocol [ ]. .. The final PCR product was cloned into pEX18Ap using Article Title: Assessing genotype-phenotype associations in three dorsal colour morphs in the meadow spittlebug Philaenus spumarius (L.) (Hemiptera: Aphrophoridae) using genomic and transcriptomic resources Article Snippet: Genomic DNA was extracted using the DNeasy Blood & Tissue Kit (Qiagen). .. Three RAD libraries with twelve individuals each were prepared following a modified RAD sequencing protocol [ ], using Article Title: Reassessment of QTLs for Late Blight Resistance in the Tomato Accession L3708 Using a Restriction Site Associated DNA (RAD) Linkage Map and Highly Aggressive Isolates of Phytophthora infestans Article Snippet: Total genomic DNA was isolated from young tomato leaves using a modified CTAB method , and further purified using the DNeasy Blood & Tissue Kit (QIAGEN, Venlo, Netherland) following manufacturer's instructions. .. For each sample, 1 µg gDNA was digested with 20 units of Transformation Assay:Article Title: PsrA controls the synthesis of the Pseudomonas aeruginosa quinolone signal via repression of the FadE homolog, PA0506 Article Snippet: The final PCR product was cloned into pEX18Ap using Derivative Assay:Article Title: PsrA controls the synthesis of the Pseudomonas aeruginosa quinolone signal via repression of the FadE homolog, PA0506 Article Snippet: Mutant P . aeruginosa strains were derived by a modified version of a previously published protocol [ ]. .. The final PCR product was cloned into pEX18Ap using Ligation:Article Title: Construction of a high-density linkage map and fine mapping of QTL for growth in Asian seabass Article Snippet: 200 ng of DNA from each sample was digested with 20 units of Article Title: The Pattern and Distribution of Induced Mutations in J. curcas Using Reduced Representation Sequencing Article Snippet: 200 ng of genomic DNA were digested with 10 U Article Title: Special features of RAD Sequencing data: implications for genotyping Article Snippet: PstI-digested RAD libraries were prepared following the study by Baird et al . , using 20 units of Article Title: Assessing genotype-phenotype associations in three dorsal colour morphs in the meadow spittlebug Philaenus spumarius (L.) (Hemiptera: Aphrophoridae) using genomic and transcriptomic resources Article Snippet: Three RAD libraries with twelve individuals each were prepared following a modified RAD sequencing protocol [ ], using Article Title: Multispecies Outcomes of Sympatric Speciation after Admixture with the Source Population in Two Radiations of Nicaraguan Crater Lake Cichlids Article Snippet: Briefly, for each individual 600 ng DNA template were digested with the Article Title: Ancient genomic variation underlies repeated ecological adaptation in young stickleback populations Article Snippet: To identify sufficient sequence variation at a RAD locus, and to simplify downstream sequence processing and analysis, we took advantage of longer sequencing reads available on newer Illumina platforms and the phase information captured by paired‐end sequencing. .. We generated RAD libraries from these samples using the single‐digest sheared RAD protocol from Baird et al. (Baird et al. ) with the following specifications and adjustments: 1 μg of genomic DNA per fish was digested with the Article Title: Mapping QTL for the traits associated with heat tolerance in wheat (Triticum aestivum L.) Article Snippet: Bin markers were developed using a genotype by sequencing (GBS) approach [ ]. .. DNA was isolated from F10 plants leaves and digested by Article Title: The Pattern and Distribution of Induced Mutations in J. curcas Using Reduced Representation Sequencing Article Snippet: 200 ng of genomic DNA were digested with 10 U Article Title: Whole Genome Amplification and Reduced-Representation Genome Sequencing of Schistosoma japonicum Miracidia Article Snippet: Adult worm DNA or whole-genome-amplified miracidium DNA was digested with two restriction enzymes, PstI -HF (New England Biolabs (NEB) Article Title: Analysis of genetic polymorphisms and tropism in East African Leishmania donovani by Amplified Fragment Length Polymorphism and kDNA minicircle sequencing Article Snippet: Paragraph title: 2.6.1. Restriction digestion and ligation of adaptors ... Following digestion with TaqI (10 U, New England Biolabs®), the mixture was cooled on ice, and then Article Title: Fine mapping QTL for resistance to VNN disease using a high-density linkage map in Asian seabass Article Snippet: In brief, 200 ng of each DNA was digested with 20 units of restriction enzyme of Generated:Article Title: PsrA controls the synthesis of the Pseudomonas aeruginosa quinolone signal via repression of the FadE homolog, PA0506 Article Snippet: The mutant alleles were generated by using a splicing-by-overlapping PCR extension of primers [ ]. .. The final PCR product was cloned into pEX18Ap using Article Title: Multispecies Outcomes of Sympatric Speciation after Admixture with the Source Population in Two Radiations of Nicaraguan Crater Lake Cichlids Article Snippet: Genomic data were generated using double-digest RAD sequencing [ ] following an in-house protocol [ ] with minor modifications. .. Briefly, for each individual 600 ng DNA template were digested with the Article Title: Ancient genomic variation underlies repeated ecological adaptation in young stickleback populations Article Snippet: To identify sufficient sequence variation at a RAD locus, and to simplify downstream sequence processing and analysis, we took advantage of longer sequencing reads available on newer Illumina platforms and the phase information captured by paired‐end sequencing. .. We generated RAD libraries from these samples using the single‐digest sheared RAD protocol from Baird et al. (Baird et al. ) with the following specifications and adjustments: 1 μg of genomic DNA per fish was digested with the DNA Sequencing:Article Title: PsrA controls the synthesis of the Pseudomonas aeruginosa quinolone signal via repression of the FadE homolog, PA0506 Article Snippet: The final PCR product was cloned into pEX18Ap using Sequencing:Article Title: Construction of a high-density linkage map and fine mapping of QTL for growth in Asian seabass Article Snippet: Paragraph title: RAD library preparation and sequencing ... 200 ng of DNA from each sample was digested with 20 units of Article Title: Mobius Assembly: A versatile Golden-Gate framework towards universal DNA assembly Article Snippet: Subsequently the pSB1C3/pSB1K3 backbones and the Golden Gate cassettes were digested with EcoRI-HF and Article Title: PsrA controls the synthesis of the Pseudomonas aeruginosa quinolone signal via repression of the FadE homolog, PA0506 Article Snippet: The alleles were constructed to harbor in-frame deletions in the DNA coding sequence corresponding to amino acids 18 to 226 for psrA (89% of protein sequence), 19 to 585 for PA0506 (94% of protein sequence), 36 to 553 for PA0507 (86% of protein sequence), and 38 to 535 for PA0508 (86% of protein sequence). .. The final PCR product was cloned into pEX18Ap using Article Title: Assessing genotype-phenotype associations in three dorsal colour morphs in the meadow spittlebug Philaenus spumarius (L.) (Hemiptera: Aphrophoridae) using genomic and transcriptomic resources Article Snippet: Genomic DNA was extracted using the DNeasy Blood & Tissue Kit (Qiagen). .. Three RAD libraries with twelve individuals each were prepared following a modified RAD sequencing protocol [ ], using Article Title: A high-density genetic map reveals variation in recombination rate across the genome of Daphnia magna Article Snippet: Paragraph title: RAD library preparation and sequencing ... Specifically, 1 μg of genomic DNA from each sample was digested with the Article Title: Multispecies Outcomes of Sympatric Speciation after Admixture with the Source Population in Two Radiations of Nicaraguan Crater Lake Cichlids Article Snippet: Paragraph title: Sampling and ddRAD sequencing ... Briefly, for each individual 600 ng DNA template were digested with the Article Title: Genomic diversity in switchgrass (Panicum virgatum): from the continental scale to a dune landscape Article Snippet: The results from Sequencing Run 1 indicated that digestion with ApeKI (recognition site: G^CWGC) did not reduce representation of the switchgrass genome to the desired level, so subsequent samples were digested with PstI (recognition site: CTGCA^G), which has a longer recognition site. .. To produce the second set of RRLs (Sequencing run 2, ), genomic DNA (5.0 ug per sample) was digested with Article Title: Ancient genomic variation underlies repeated ecological adaptation in young stickleback populations Article Snippet: To identify sufficient sequence variation at a RAD locus, and to simplify downstream sequence processing and analysis, we took advantage of longer sequencing reads available on newer Illumina platforms and the phase information captured by paired‐end sequencing. .. We generated RAD libraries from these samples using the single‐digest sheared RAD protocol from Baird et al. (Baird et al. ) with the following specifications and adjustments: 1 μg of genomic DNA per fish was digested with the Article Title: Mapping QTL for the traits associated with heat tolerance in wheat (Triticum aestivum L.) Article Snippet: Bin markers were developed using a genotype by sequencing (GBS) approach [ ]. .. DNA was isolated from F10 plants leaves and digested by Article Title: Fine mapping QTL for resistance to VNN disease using a high-density linkage map in Asian seabass Article Snippet: Paragraph title: Sequencing library preparation and next generation sequencing ... In brief, 200 ng of each DNA was digested with 20 units of restriction enzyme of Article Title: Efficiency of multi-trait, indirect, and trait-assisted genomic selection for improvement of biomass sorghum Article Snippet: Illumina libraries were created using two pairs of restriction enzymes: PstI-HF/HinP1I and Binding Assay:Article Title: Downstream promoter interactions of TFIID TAFs facilitate transcription reinitiation Article Snippet: Paragraph title: Immobilized template binding and quantitative mass spectrometry ... Downstream DNA and bound proteins were released with 120 U of SspI-HF or DNA Extraction:Article Title: The Chromosomal Association of the Smc5/6 Complex Depends on Cohesion and Predicts the Level of Sister Chromatid Entanglement Article Snippet: Genomic DNA isolation to study replication intermediates was performed according to . .. Digestion was performed using Nucleic Acid Electrophoresis:Article Title: Multispecies Outcomes of Sympatric Speciation after Admixture with the Source Population in Two Radiations of Nicaraguan Crater Lake Cichlids Article Snippet: Briefly, for each individual 600 ng DNA template were digested with the Article Title: Evolved Populations of Shigella flexneri Phage Sf6 Acquire Large Deletions, Altered Genomic Architecture, and Faster Life Cycles Article Snippet: Primers were constructed with Primer3Plus within Sf6 genes 1 (5′-AAGACTTCGCGTTGATACCC-3′, 5′-GTGCTCATCGGTGATGTTTC-3′), 5 (5′-TGCAACAGCAGACCAAACAG-3′, 5′-AAAGCGTAACCGTCACATCG-3′), 44 (5′-GACGCTGATGACGGCTATCA-3′, 5′-GCGTGTCGTCGATGTAAAGC-3′), and 61 (5′-GTTTGCAATGGCGTACCTTC-3′, 5′-CACATCGTTGCGTCGATTAC-3′) and were used with an in-house SYBR Green master mix as described ( ). .. Single restriction enzyme digests used BamHI HF and Magnetic Beads:Article Title: Special features of RAD Sequencing data: implications for genotyping Article Snippet: PstI-digested RAD libraries were prepared following the study by Baird et al . , using 20 units of Article Title: Assessing genotype-phenotype associations in three dorsal colour morphs in the meadow spittlebug Philaenus spumarius (L.) (Hemiptera: Aphrophoridae) using genomic and transcriptomic resources Article Snippet: Three RAD libraries with twelve individuals each were prepared following a modified RAD sequencing protocol [ ], using Mutagenesis:Article Title: PsrA controls the synthesis of the Pseudomonas aeruginosa quinolone signal via repression of the FadE homolog, PA0506 Article Snippet: Paragraph title: Generation of mutant strains and plasmids ... The final PCR product was cloned into pEX18Ap using Isolation:Article Title: The Chromosomal Association of the Smc5/6 Complex Depends on Cohesion and Predicts the Level of Sister Chromatid Entanglement Article Snippet: Isolation of genomic DNA with CTAB extraction to preserve X-shape structures was performed according to . .. Digestion was performed using Article Title: Special features of RAD Sequencing data: implications for genotyping Article Snippet: Genomic DNA (gDNA) was isolated from frozen worms using the DNeasy Blood and Tissue Kit (QIAGEN). .. PstI-digested RAD libraries were prepared following the study by Baird et al . , using 20 units of Article Title: Reassessment of QTLs for Late Blight Resistance in the Tomato Accession L3708 Using a Restriction Site Associated DNA (RAD) Linkage Map and Highly Aggressive Isolates of Phytophthora infestans Article Snippet: Total genomic DNA was isolated from young tomato leaves using a modified CTAB method , and further purified using the DNeasy Blood & Tissue Kit (QIAGEN, Venlo, Netherland) following manufacturer's instructions. .. For each sample, 1 µg gDNA was digested with 20 units of Article Title: Mapping QTL for the traits associated with heat tolerance in wheat (Triticum aestivum L.) Article Snippet: Bin markers were developed using a genotype by sequencing (GBS) approach [ ]. .. DNA was isolated from F10 plants leaves and digested by Purification:Article Title: Construction of a high-density linkage map and fine mapping of QTL for growth in Asian seabass Article Snippet: 200 ng of DNA from each sample was digested with 20 units of Article Title: Mobius Assembly: A versatile Golden-Gate framework towards universal DNA assembly Article Snippet: They were then amplified using Q5 DNA polymerase (NEB) with primers bearing EcoRI , AarI , BsaI , and PstI restriction sites and 4 bp overhangs. .. Subsequently the pSB1C3/pSB1K3 backbones and the Golden Gate cassettes were digested with EcoRI-HF and Article Title: Special features of RAD Sequencing data: implications for genotyping Article Snippet: PstI-digested RAD libraries were prepared following the study by Baird et al . , using 20 units of Article Title: Assessing genotype-phenotype associations in three dorsal colour morphs in the meadow spittlebug Philaenus spumarius (L.) (Hemiptera: Aphrophoridae) using genomic and transcriptomic resources Article Snippet: Three RAD libraries with twelve individuals each were prepared following a modified RAD sequencing protocol [ ], using Article Title: Reassessment of QTLs for Late Blight Resistance in the Tomato Accession L3708 Using a Restriction Site Associated DNA (RAD) Linkage Map and Highly Aggressive Isolates of Phytophthora infestans Article Snippet: Total genomic DNA was isolated from young tomato leaves using a modified CTAB method , and further purified using the DNeasy Blood & Tissue Kit (QIAGEN, Venlo, Netherland) following manufacturer's instructions. .. For each sample, 1 µg gDNA was digested with 20 units of Article Title: Whole Genome Amplification and Reduced-Representation Genome Sequencing of Schistosoma japonicum Miracidia Article Snippet: Adult worm DNA or whole-genome-amplified miracidium DNA was digested with two restriction enzymes, PstI -HF (New England Biolabs (NEB) Article Title: Fine mapping QTL for resistance to VNN disease using a high-density linkage map in Asian seabass Article Snippet: In brief, 200 ng of each DNA was digested with 20 units of restriction enzyme of Polymerase Chain Reaction:Article Title: Construction of a high-density linkage map and fine mapping of QTL for growth in Asian seabass Article Snippet: 200 ng of DNA from each sample was digested with 20 units of Article Title: Mobius Assembly: A versatile Golden-Gate framework towards universal DNA assembly Article Snippet: They were then amplified using Q5 DNA polymerase (NEB) with primers bearing EcoRI , AarI , BsaI , and PstI restriction sites and 4 bp overhangs. .. Subsequently the pSB1C3/pSB1K3 backbones and the Golden Gate cassettes were digested with EcoRI-HF and Article Title: PsrA controls the synthesis of the Pseudomonas aeruginosa quinolone signal via repression of the FadE homolog, PA0506 Article Snippet: Strain PAO1 chromosomal DNA was utilized as template for the PCR. .. The final PCR product was cloned into pEX18Ap using Article Title: The Pattern and Distribution of Induced Mutations in J. curcas Using Reduced Representation Sequencing Article Snippet: 200 ng of genomic DNA were digested with 10 U Article Title: Special features of RAD Sequencing data: implications for genotyping Article Snippet: PstI-digested RAD libraries were prepared following the study by Baird et al . , using 20 units of Article Title: Assessing genotype-phenotype associations in three dorsal colour morphs in the meadow spittlebug Philaenus spumarius (L.) (Hemiptera: Aphrophoridae) using genomic and transcriptomic resources Article Snippet: Three RAD libraries with twelve individuals each were prepared following a modified RAD sequencing protocol [ ], using Article Title: Reassessment of QTLs for Late Blight Resistance in the Tomato Accession L3708 Using a Restriction Site Associated DNA (RAD) Linkage Map and Highly Aggressive Isolates of Phytophthora infestans Article Snippet: For each sample, 1 µg gDNA was digested with 20 units of Article Title: The Pattern and Distribution of Induced Mutations in J. curcas Using Reduced Representation Sequencing Article Snippet: 200 ng of genomic DNA were digested with 10 U Article Title: Whole Genome Amplification and Reduced-Representation Genome Sequencing of Schistosoma japonicum Miracidia Article Snippet: Adult worm DNA or whole-genome-amplified miracidium DNA was digested with two restriction enzymes, PstI -HF (New England Biolabs (NEB) Article Title: Analysis of genetic polymorphisms and tropism in East African Leishmania donovani by Amplified Fragment Length Polymorphism and kDNA minicircle sequencing Article Snippet: Following digestion with TaqI (10 U, New England Biolabs®), the mixture was cooled on ice, and then Article Title: Fine mapping QTL for resistance to VNN disease using a high-density linkage map in Asian seabass Article Snippet: In brief, 200 ng of each DNA was digested with 20 units of restriction enzyme of Article Title: Efficiency of multi-trait, indirect, and trait-assisted genomic selection for improvement of biomass sorghum Article Snippet: Illumina libraries were created using two pairs of restriction enzymes: PstI-HF/HinP1I and Plasmid Preparation:Article Title: Mobius Assembly: A versatile Golden-Gate framework towards universal DNA assembly Article Snippet: Paragraph title: Part and vector generation ... Subsequently the pSB1C3/pSB1K3 backbones and the Golden Gate cassettes were digested with EcoRI-HF and Selection:Article Title: Construction of a high-density linkage map and fine mapping of QTL for growth in Asian seabass Article Snippet: 200 ng of DNA from each sample was digested with 20 units of Article Title: Fine mapping QTL for resistance to VNN disease using a high-density linkage map in Asian seabass Article Snippet: In brief, 200 ng of each DNA was digested with 20 units of restriction enzyme of Article Title: Efficiency of multi-trait, indirect, and trait-assisted genomic selection for improvement of biomass sorghum Article Snippet: Illumina libraries were created using two pairs of restriction enzymes: PstI-HF/HinP1I and Next-Generation Sequencing:Article Title: Fine mapping QTL for resistance to VNN disease using a high-density linkage map in Asian seabass Article Snippet: Paragraph title: Sequencing library preparation and next generation sequencing ... In brief, 200 ng of each DNA was digested with 20 units of restriction enzyme of Quantitation Assay:Article Title: Downstream promoter interactions of TFIID TAFs facilitate transcription reinitiation Article Snippet: Downstream DNA and bound proteins were released with 120 U of SspI-HF or Sampling:Article Title: Multispecies Outcomes of Sympatric Speciation after Admixture with the Source Population in Two Radiations of Nicaraguan Crater Lake Cichlids Article Snippet: Paragraph title: Sampling and ddRAD sequencing ... Briefly, for each individual 600 ng DNA template were digested with the Produced:Article Title: Construction of a high-density linkage map and fine mapping of QTL for growth in Asian seabass Article Snippet: 200 ng of DNA from each sample was digested with 20 units of Concentration Assay:Article Title: Construction of a high-density linkage map and fine mapping of QTL for growth in Asian seabass Article Snippet: Genomic DNA concentration was measured using Qubit® assays (Life Technologies, USA). .. 200 ng of DNA from each sample was digested with 20 units of Two-Dimensional Gel Electrophoresis:Article Title: The Chromosomal Association of the Smc5/6 Complex Depends on Cohesion and Predicts the Level of Sister Chromatid Entanglement Article Snippet: Paragraph title: Two-dimensional gel electrophoresis ... Digestion was performed using High Throughput Screening Assay:Article Title: Genomic diversity in switchgrass (Panicum virgatum): from the continental scale to a dune landscape Article Snippet: Paragraph title: Library preparation and high-throughput sequencing ... To produce the second set of RRLs (Sequencing run 2, ), genomic DNA (5.0 ug per sample) was digested with Fluorescence In Situ Hybridization:Article Title: Multispecies Outcomes of Sympatric Speciation after Admixture with the Source Population in Two Radiations of Nicaraguan Crater Lake Cichlids Article Snippet: Briefly, for each individual 600 ng DNA template were digested with the restriction enzymes PstI-HF (NEB) and MspI (NEB). .. Briefly, for each individual 600 ng DNA template were digested with the Article Title: Ancient genomic variation underlies repeated ecological adaptation in young stickleback populations Article Snippet: To identify sufficient sequence variation at a RAD locus, and to simplify downstream sequence processing and analysis, we took advantage of longer sequencing reads available on newer Illumina platforms and the phase information captured by paired‐end sequencing. .. We generated RAD libraries from these samples using the single‐digest sheared RAD protocol from Baird et al. (Baird et al. ) with the following specifications and adjustments: 1 μg of genomic DNA per fish was digested with the |