spei hf  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 98
    Name:
    SpeI HF
    Description:
    SpeI HF 2 500 units
    Catalog Number:
    r3133l
    Price:
    282
    Size:
    2 500 units
    Category:
    Restriction Enzymes
    Buy from Supplier


    Structured Review

    New England Biolabs spei hf
    SpeI HF
    SpeI HF 2 500 units
    https://www.bioz.com/result/spei hf/product/New England Biolabs
    Average 98 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    spei hf - by Bioz Stars, 2020-08
    98/100 stars

    Images

    Related Articles

    Clone Assay:

    Article Title: Developmental Testicular Expression, Cloning, and Characterization of Rat HDAC6 In Silico
    Article Snippet: .. Positive clones were confirmed by restriction digestion of the isolated plasmid with EcoRI HF and SpeI-HF (New England Biolabs, Massachusetts, USA) sequentially, at 37°C overnight and electrophoresing the digested products on 0.8% agarose gels. .. Overlapping primers were designed for hdac6 ORF using Primer 3 software to amplify and sequence the 3.56 kb gene by Sanger sequencing ( ).

    Article Title: High throughput screening identifies SOX2 as a Super Pioneer Factor that inhibits DNA methylation maintenance at its binding sites
    Article Snippet: .. For each motif, forward and reverse oligomers were annealed and cloned into the FR1 fragment by directional cloning using the restriction enzymes SphI (NEB, R3133L) and NheI (NEB, R3131L). .. To create the plasmid libraries, single-motifs containing plasmids were mixed in equimolar fashion and co-precipitated before RMCE or M.SssI treatment.

    In Vitro:

    Article Title: Structure of a human translation termination complex
    Article Snippet: .. In vitro transcription Plasmids were linearized with SpeI-HF (NEB) in CutSmart (NEB) buffer at 37°C overnight and purified (QIAquick PCR purification kit (Qiagen)) before in vitro transcription (tc) to ensure the right length of the mRNA product. .. The final mRNA construct encoded a CrPV IGR IRES sequence for translation initiation, an N-terminal HA- and (His)6 -tag, the well characterized DP75 dipeptidyl-aminopeptidase B (DPAPB) , the hCMV uORF2 stalling sequence and a polyA-tail.

    Ligation:

    Article Title: Efficient single-copy HDR by 5’ modified long dsDNA donors
    Article Snippet: .. The respective restriction enzyme was used to digest the amplicons (5’HF: SalI HF (New England Biolabs), AgeI HF (New England Biolabs); mgfp-flexible linker : AgeI HF (New England Biolabs), SpeI HF (New England Biolabs); 3’HF: SpeI HF (New England Biolabs), NotI HF (New England Biolabs)) followed by gel purification (Analytik Jena) and ligation into pCS2+ ( ) (digested with SalI HF (New England Biolabs), NotI HF (New England Biolabs)). .. The rx1 gfp plasmid was cloned with homology flanks (5’HF 430 bp, primers rx1 5’HF f/rx1 5’HF r; 3’ HF 508 bp, rx1 3’HF f/rx1 3’HF r) that were PCR amplified with Q5 polymerase (New England Biolabs, 30 cycles) from wild-type medaka genomic DNA.

    Article Title: Efficient single-copy HDR by 5’ modified long dsDNA donors
    Article Snippet: .. The respective restriction enzyme was used to digest the amplicons (5’HF: SalI HF (New England Biolabs), AgeI HF (New England Biolabs); mgfp-flexible linker : AgeI HF (New England Biolabs), SpeI HF (New England Biolabs); 3’HF: SpeI HF (New England Biolabs), NotI HF (New England Biolabs)) followed by gel purification (Analytik Jena) and ligation into pCS2+ ( ) (digested with SalI HF (New England Biolabs), NotI HF (New England Biolabs)). .. The rx1 gfp plasmid was cloned with homology flanks (5’HF 430 bp, primers rx1 5’HF f/rx1 5’HF r; 3’ HF 508 bp, rx1 3’HF f/rx1 3’HF r) that were PCR amplified with Q5 polymerase (New England Biolabs, 30 cycles) from wild-type medaka genomic DNA.

    Isolation:

    Article Title: Developmental Testicular Expression, Cloning, and Characterization of Rat HDAC6 In Silico
    Article Snippet: .. Positive clones were confirmed by restriction digestion of the isolated plasmid with EcoRI HF and SpeI-HF (New England Biolabs, Massachusetts, USA) sequentially, at 37°C overnight and electrophoresing the digested products on 0.8% agarose gels. .. Overlapping primers were designed for hdac6 ORF using Primer 3 software to amplify and sequence the 3.56 kb gene by Sanger sequencing ( ).

    Purification:

    Article Title: Structure of a human translation termination complex
    Article Snippet: .. In vitro transcription Plasmids were linearized with SpeI-HF (NEB) in CutSmart (NEB) buffer at 37°C overnight and purified (QIAquick PCR purification kit (Qiagen)) before in vitro transcription (tc) to ensure the right length of the mRNA product. .. The final mRNA construct encoded a CrPV IGR IRES sequence for translation initiation, an N-terminal HA- and (His)6 -tag, the well characterized DP75 dipeptidyl-aminopeptidase B (DPAPB) , the hCMV uORF2 stalling sequence and a polyA-tail.

    Article Title: Efficient targeted integration directed by short homology in zebrafish and mammalian cells
    Article Snippet: .. Fragments were digested with SpeI-HF and XhoI (NEB) and following column purification with the Qiagen miniprep protocol, were ligated to the plasmid backbone with T4 ligase (Fisher). .. The Gal4/VP16 coding sequence and zebrafish β-actin 3’ untranslated region was amplified from vector pDB783 ( ) with primers F-2A-Gal4-BamHI and R-Gal4-NcoI to add a 2A peptide to the 5’ end of the Gav4Vp16 cDNA.

    Polymerase Chain Reaction:

    Article Title: Structure of a human translation termination complex
    Article Snippet: .. In vitro transcription Plasmids were linearized with SpeI-HF (NEB) in CutSmart (NEB) buffer at 37°C overnight and purified (QIAquick PCR purification kit (Qiagen)) before in vitro transcription (tc) to ensure the right length of the mRNA product. .. The final mRNA construct encoded a CrPV IGR IRES sequence for translation initiation, an N-terminal HA- and (His)6 -tag, the well characterized DP75 dipeptidyl-aminopeptidase B (DPAPB) , the hCMV uORF2 stalling sequence and a polyA-tail.

    Plasmid Preparation:

    Article Title: Developmental Testicular Expression, Cloning, and Characterization of Rat HDAC6 In Silico
    Article Snippet: .. Positive clones were confirmed by restriction digestion of the isolated plasmid with EcoRI HF and SpeI-HF (New England Biolabs, Massachusetts, USA) sequentially, at 37°C overnight and electrophoresing the digested products on 0.8% agarose gels. .. Overlapping primers were designed for hdac6 ORF using Primer 3 software to amplify and sequence the 3.56 kb gene by Sanger sequencing ( ).

    Article Title: Efficient targeted integration directed by short homology in zebrafish and mammalian cells
    Article Snippet: .. Fragments were digested with SpeI-HF and XhoI (NEB) and following column purification with the Qiagen miniprep protocol, were ligated to the plasmid backbone with T4 ligase (Fisher). .. The Gal4/VP16 coding sequence and zebrafish β-actin 3’ untranslated region was amplified from vector pDB783 ( ) with primers F-2A-Gal4-BamHI and R-Gal4-NcoI to add a 2A peptide to the 5’ end of the Gav4Vp16 cDNA.

    Gel Purification:

    Article Title: Efficient single-copy HDR by 5’ modified long dsDNA donors
    Article Snippet: .. The respective restriction enzyme was used to digest the amplicons (5’HF: SalI HF (New England Biolabs), AgeI HF (New England Biolabs); mgfp-flexible linker : AgeI HF (New England Biolabs), SpeI HF (New England Biolabs); 3’HF: SpeI HF (New England Biolabs), NotI HF (New England Biolabs)) followed by gel purification (Analytik Jena) and ligation into pCS2+ ( ) (digested with SalI HF (New England Biolabs), NotI HF (New England Biolabs)). .. The rx1 gfp plasmid was cloned with homology flanks (5’HF 430 bp, primers rx1 5’HF f/rx1 5’HF r; 3’ HF 508 bp, rx1 3’HF f/rx1 3’HF r) that were PCR amplified with Q5 polymerase (New England Biolabs, 30 cycles) from wild-type medaka genomic DNA.

    Article Title: Efficient single-copy HDR by 5’ modified long dsDNA donors
    Article Snippet: .. The respective restriction enzyme was used to digest the amplicons (5’HF: SalI HF (New England Biolabs), AgeI HF (New England Biolabs); mgfp-flexible linker : AgeI HF (New England Biolabs), SpeI HF (New England Biolabs); 3’HF: SpeI HF (New England Biolabs), NotI HF (New England Biolabs)) followed by gel purification (Analytik Jena) and ligation into pCS2+ ( ) (digested with SalI HF (New England Biolabs), NotI HF (New England Biolabs)). .. The rx1 gfp plasmid was cloned with homology flanks (5’HF 430 bp, primers rx1 5’HF f/rx1 5’HF r; 3’ HF 508 bp, rx1 3’HF f/rx1 3’HF r) that were PCR amplified with Q5 polymerase (New England Biolabs, 30 cycles) from wild-type medaka genomic DNA.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 98
    New England Biolabs spei hf
    Spei Hf, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/spei hf/product/New England Biolabs
    Average 98 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    spei hf - by Bioz Stars, 2020-08
    98/100 stars
      Buy from Supplier

    Image Search Results