paqci restriction enzyme  (New England Biolabs)


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    New England Biolabs paqci restriction enzyme
    Paqci Restriction Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 1 article reviews
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    paqci restriction enzyme  (New England Biolabs)


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    New England Biolabs paqci restriction enzyme
    Paqci Restriction Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/paqci restriction enzyme/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
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    isoschizomer paqci  (New England Biolabs)


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    New England Biolabs isoschizomer paqci
    Isoschizomer Paqci, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    isoschizomer paqci  (New England Biolabs)


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    New England Biolabs isoschizomer paqci
    Isoschizomer Paqci, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 1 article reviews
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    paqci  (New England Biolabs)


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    New England Biolabs paqci
    Paqci, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/paqci/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
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    paqci  (New England Biolabs)


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    New England Biolabs paqci
    Paqci, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/paqci/product/New England Biolabs
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    product with paqci  (New England Biolabs)


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    New England Biolabs product with paqci
    The feature of CRISPR/LbCas12a toolbox. a) the schematic diagram <t>of</t> <t>pMM002P.</t> The uracil phosphoribosyltransferase ( upt ) from M. maripaludis S2 as a counter-selective marker was co-expressed with the codon-optimized puromycin N-acetyltransferase ( pac ) under P mcr promoter (16). The promoter P hdr from Methanococcus voltae A3 was used to drive the expression of LbCas12a (25). The histone promoter P hmv from M. voltae A3 was used to express crRNA. Two <t>PaqCI</t> sties were placed between the direct repeat sequence and the synthetic terminator in the opposite direction for spacer fusion. The crRNA of the CRISPR tool plasmid pMM002P containing two PaqCI sites cannot target onto the native chromosome. An MreI restriction site was assigned between the promoters, P hdr and P hmv , for RF insertion if needed. b) cleavage test of pMM002P.
    Product With Paqci, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/product with paqci/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
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    1) Product Images from "Efficient CRISPR/Cas12a-based genome editing toolbox for metabolic engineering in Methanococcus maripaludis"

    Article Title: Efficient CRISPR/Cas12a-based genome editing toolbox for metabolic engineering in Methanococcus maripaludis

    Journal: bioRxiv

    doi: 10.1101/2021.12.29.474413

    The feature of CRISPR/LbCas12a toolbox. a) the schematic diagram of pMM002P. The uracil phosphoribosyltransferase ( upt ) from M. maripaludis S2 as a counter-selective marker was co-expressed with the codon-optimized puromycin N-acetyltransferase ( pac ) under P mcr promoter (16). The promoter P hdr from Methanococcus voltae A3 was used to drive the expression of LbCas12a (25). The histone promoter P hmv from M. voltae A3 was used to express crRNA. Two PaqCI sties were placed between the direct repeat sequence and the synthetic terminator in the opposite direction for spacer fusion. The crRNA of the CRISPR tool plasmid pMM002P containing two PaqCI sites cannot target onto the native chromosome. An MreI restriction site was assigned between the promoters, P hdr and P hmv , for RF insertion if needed. b) cleavage test of pMM002P.
    Figure Legend Snippet: The feature of CRISPR/LbCas12a toolbox. a) the schematic diagram of pMM002P. The uracil phosphoribosyltransferase ( upt ) from M. maripaludis S2 as a counter-selective marker was co-expressed with the codon-optimized puromycin N-acetyltransferase ( pac ) under P mcr promoter (16). The promoter P hdr from Methanococcus voltae A3 was used to drive the expression of LbCas12a (25). The histone promoter P hmv from M. voltae A3 was used to express crRNA. Two PaqCI sties were placed between the direct repeat sequence and the synthetic terminator in the opposite direction for spacer fusion. The crRNA of the CRISPR tool plasmid pMM002P containing two PaqCI sites cannot target onto the native chromosome. An MreI restriction site was assigned between the promoters, P hdr and P hmv , for RF insertion if needed. b) cleavage test of pMM002P.

    Techniques Used: CRISPR, Marker, Expressing, Sequencing, Plasmid Preparation

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    New England Biolabs paqci restriction enzyme
    Paqci Restriction Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/paqci restriction enzyme/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
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    New England Biolabs isoschizomer paqci
    Isoschizomer Paqci, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/isoschizomer paqci/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
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    94
    New England Biolabs paqci
    Paqci, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    New England Biolabs product with paqci
    The feature of CRISPR/LbCas12a toolbox. a) the schematic diagram <t>of</t> <t>pMM002P.</t> The uracil phosphoribosyltransferase ( upt ) from M. maripaludis S2 as a counter-selective marker was co-expressed with the codon-optimized puromycin N-acetyltransferase ( pac ) under P mcr promoter (16). The promoter P hdr from Methanococcus voltae A3 was used to drive the expression of LbCas12a (25). The histone promoter P hmv from M. voltae A3 was used to express crRNA. Two <t>PaqCI</t> sties were placed between the direct repeat sequence and the synthetic terminator in the opposite direction for spacer fusion. The crRNA of the CRISPR tool plasmid pMM002P containing two PaqCI sites cannot target onto the native chromosome. An MreI restriction site was assigned between the promoters, P hdr and P hmv , for RF insertion if needed. b) cleavage test of pMM002P.
    Product With Paqci, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    The feature of CRISPR/LbCas12a toolbox. a) the schematic diagram of pMM002P. The uracil phosphoribosyltransferase ( upt ) from M. maripaludis S2 as a counter-selective marker was co-expressed with the codon-optimized puromycin N-acetyltransferase ( pac ) under P mcr promoter (16). The promoter P hdr from Methanococcus voltae A3 was used to drive the expression of LbCas12a (25). The histone promoter P hmv from M. voltae A3 was used to express crRNA. Two PaqCI sties were placed between the direct repeat sequence and the synthetic terminator in the opposite direction for spacer fusion. The crRNA of the CRISPR tool plasmid pMM002P containing two PaqCI sites cannot target onto the native chromosome. An MreI restriction site was assigned between the promoters, P hdr and P hmv , for RF insertion if needed. b) cleavage test of pMM002P.

    Journal: bioRxiv

    Article Title: Efficient CRISPR/Cas12a-based genome editing toolbox for metabolic engineering in Methanococcus maripaludis

    doi: 10.1101/2021.12.29.474413

    Figure Lengend Snippet: The feature of CRISPR/LbCas12a toolbox. a) the schematic diagram of pMM002P. The uracil phosphoribosyltransferase ( upt ) from M. maripaludis S2 as a counter-selective marker was co-expressed with the codon-optimized puromycin N-acetyltransferase ( pac ) under P mcr promoter (16). The promoter P hdr from Methanococcus voltae A3 was used to drive the expression of LbCas12a (25). The histone promoter P hmv from M. voltae A3 was used to express crRNA. Two PaqCI sties were placed between the direct repeat sequence and the synthetic terminator in the opposite direction for spacer fusion. The crRNA of the CRISPR tool plasmid pMM002P containing two PaqCI sites cannot target onto the native chromosome. An MreI restriction site was assigned between the promoters, P hdr and P hmv , for RF insertion if needed. b) cleavage test of pMM002P.

    Article Snippet: All the cleavage plasmids were constructed as follow: (1) design the forward primer as “5’-AGAT+(23nt guide sequence)”, design the reverse primer as “5’-TATC+(23nt reverse complement guide sequence)” (2) anneal the two primers which contains the gRNA and have 4 nt overhang at 5’-end (3) ligate the annealing product with PaqCI (New England Biolabs) digested pMM002P.

    Techniques: CRISPR, Marker, Expressing, Sequencing, Plasmid Preparation