esp3i (New England Biolabs)
New England Biolabs is a verified supplier 
New England Biolabs manufactures this product 
95
|
Name:
Esp3I
Description:
Catalog Number:
R0734
Price:
350
Category:
Restriction Enzymes
Applications:
DNA Manipulation
Size:
1500 units
|
Buy from Supplier |
Structured Review
New England Biolabs
esp3i
https://www.bioz.com/result/esp3i/product/New England Biolabs
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
https://www.bioz.com/result/esp3i/product/New England Biolabs
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
esp3i - by Bioz Stars,
2021-07
95/100 stars
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Related Articles
Recombinant:Article Title: CRTC1/MAML2 directs a PGC-1α-IGF-1 circuit that confers vulnerability to PPARγ inhibition Article Snippet: The pgRNA-CKB vector (Addgene #73501) ( ) was used to constitutively express the target guide RNAs. sgRNAs were cloned into the pgRNA-CKB vector using previously published methods ( ). .. Briefly, the pgRNACKB backbone was digested with Plasmid Preparation:Article Title: N6-methyladenosine mRNA marking promotes selective translation of regulons required for human erythropoiesis Article Snippet: The reaction is incubated for 4 min at 95 °C, and then 70 °C for 10 min, followed by a slow ramp to RT over a couple of hours. .. The vector is linearized with Article Title: Knock-in and precise nucleotide substitution using near-PAMless engineered Cas9 variants in Dictyostelium discoideum Article Snippet: .. The annealed oligonucleotides (1.0 µl) were then ligated into an all-in-one vector (25 ng) via a Golden Gate digestion/ligation reaction using 70 U T4 DNA ligase and 1.5 U BpiI (Thermo) or Article Title: Fine-mapping within eQTL credible intervals by expression CROP-seq Article Snippet: .. The CROPseq-Guide-Puro plasmid [ ] (Addgene, Watertown MA, catalog number #86708, originally from Christoph Bock’s lab) was digested by Article Title: Controlling gene expression in mammalian cells using multiplexed conditional guide RNAs for Cas12a Article Snippet: Most constructs for gRNAs or strand displacement triggers were assembled using GoldenGate cloning in combination with overlapping oligos. .. DNA oligos were purchased from IDT and annealed at a concentration of 2 μM in H2 O with 2 mM MgCl2 by cooling them from 90°C to 20°C over a period of 20 min. For the GoldenGate reactions, 3 nM of plasmid, 15 nM of each overlapping oligo pair, 1 U/μl Article Title: Recombinant Antibody Production Using a Dual-Promoter Single Plasmid System Article Snippet: .. Assembly of the MD expression constructs was conducted with 75 ng destination vector and equimolar amounts of the respective fragments, 20 U Bbs I-HF, 10 U Purification:Article Title: N6-methyladenosine mRNA marking promotes selective translation of regulons required for human erythropoiesis Article Snippet: The reaction is incubated for 4 min at 95 °C, and then 70 °C for 10 min, followed by a slow ramp to RT over a couple of hours. .. The vector is linearized with Gel Extraction:Article Title: N6-methyladenosine mRNA marking promotes selective translation of regulons required for human erythropoiesis Article Snippet: The reaction is incubated for 4 min at 95 °C, and then 70 °C for 10 min, followed by a slow ramp to RT over a couple of hours. .. The vector is linearized with other:Article Title: A nanoluciferase SARS-CoV-2 for rapid neutralization testing and screening of anti-infective drugs for COVID-19 Article Snippet: F5 and F6 fragments were obtained by digesting the plasmids with enzymes Esp3I and PvuI. Concentration Assay:Article Title: Systematic Assembly of a Full-Length Infectious cDNA of Mouse Hepatitis Virus Strain A59 Article Snippet: A consensus sequence for each of the cloned fragments was determined, and a consensus clone was assembled by using restriction enzymes and standard recombinant DNA techniques. .. Each of the plasmids was grown to a high concentration, isolated, and digested, or double digested, with Article Title: Controlling gene expression in mammalian cells using multiplexed conditional guide RNAs for Cas12a Article Snippet: Most constructs for gRNAs or strand displacement triggers were assembled using GoldenGate cloning in combination with overlapping oligos. .. DNA oligos were purchased from IDT and annealed at a concentration of 2 μM in H2 O with 2 mM MgCl2 by cooling them from 90°C to 20°C over a period of 20 min. For the GoldenGate reactions, 3 nM of plasmid, 15 nM of each overlapping oligo pair, 1 U/μl Isolation:Article Title: Systematic Assembly of a Full-Length Infectious cDNA of Mouse Hepatitis Virus Strain A59 Article Snippet: A consensus sequence for each of the cloned fragments was determined, and a consensus clone was assembled by using restriction enzymes and standard recombinant DNA techniques. .. Each of the plasmids was grown to a high concentration, isolated, and digested, or double digested, with End-sequence Profiling:Article Title: Systematic Assembly of a Full-Length Infectious cDNA of Mouse Hepatitis Virus Strain A59 Article Snippet: A consensus sequence for each of the cloned fragments was determined, and a consensus clone was assembled by using restriction enzymes and standard recombinant DNA techniques. .. Each of the plasmids was grown to a high concentration, isolated, and digested, or double digested, with Expressing:Article Title: Recombinant Antibody Production Using a Dual-Promoter Single Plasmid System Article Snippet: .. Assembly of the MD expression constructs was conducted with 75 ng destination vector and equimolar amounts of the respective fragments, 20 U Bbs I-HF, 10 U Construct:Article Title: Recombinant Antibody Production Using a Dual-Promoter Single Plasmid System Article Snippet: .. Assembly of the MD expression constructs was conducted with 75 ng destination vector and equimolar amounts of the respective fragments, 20 U Bbs I-HF, 10 U |
