esp3i (New England Biolabs)


Name:
Esp3I
Description:
Esp3I 1 500 units
Catalog Number:
r0734l
Price:
343
Category:
Restriction Enzymes
Size:
1 500 units
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Esp3I 1 500 units
https://www.bioz.com/result/esp3i/product/New England Biolabs
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
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Plasmid Preparation:Article Title: Fine-mapping within eQTL credible intervals by expression CROP-seq Article Snippet: .. The CROPseq-Guide-Puro plasmid [ ] (Addgene, Watertown MA, catalog number #86708, originally from Christoph Bock’s lab) was digested by Article Title: A One-Step Process for the Construction of Phage Display scFv and VHH Libraries. Article Snippet: In this work we present a one-step cloning approach for the establishment of antibody phage display libraries relying on type IIs restriction enzymes. .. In this work we present a one-step cloning approach for the establishment of antibody phage display libraries relying on type IIs restriction enzymes. .. In this work we present a one-step cloning approach for the establishment of antibody phage display libraries relying on type IIs restriction enzymes. Article Title: Multimodal Analysis of Composition and Spatial Architecture in Human Squamous Cell Carcinoma Article Snippet: The amplified oligo pool was run on a 2% agarose gel and purified using the Zymoclean Gel Recovery kit. .. 16ng of the oligo pool was then assembled with 446ng Article Title: Versatile labeling and detection of endogenous proteins using tag-assisted split enzyme complementation Article Snippet: For generating landing pad vectors from expression vectors without the correct overhangs, an oligonucleotide stuffer was used to complete the overhangs. .. TA-splitHalo BFP plasmids were made in 10 μL reactions comprising 20 fmol Kanamycin ColE1 digested backbone, 40 fmol TA-splitHalo fusion expression vectors, 40 fmol PGK-mTagBFP2 expression vector, 10x T4 Ligase Buffer (NEB B0202S), Article Title: Versatile labeling and detection of endogenous proteins using tag-assisted split enzyme complementation Article Snippet: Expression vectors were generated in 10 μL reactions containing 20 fmol CDS backbone, 40 fmol of each part insert, 10x T4 Ligase Buffer, BsaI-HF v2.0 (NEB R3733S/L), and T7 DNA Ligase with the same cycling conditions as the part vectors. .. Landing pad (LP) vectors were generated similarly to part vectors in 10 μL reactions with 20 fmol MTK landing pad entry backbone (Addgene #123932), 40 fmol of each expression vector plasmids, 10x T4 Ligase Buffer (NEB B0202S), Article Title: A bispecific immunotweezer prevents soluble PrP oligomers and abolishes prion toxicity Article Snippet: Reaction mix was heated for 4 min at 95°C on a heating block ThermoStat (Eppendorf), then the heating block was turned off and the reaction was allowed to proceed for 30 min on the block and was then put at 4°C. .. Golden Gate assembly [ ] was used in order to clone the double-stranded DNA Oligomers into the MLM3636 plasmid, using the following reaction: 150 ng MLM3636 plasmid 1 μL double stranded oligomer ligation mix 2 μL T4 ligase buffer (New England Biolabs) 13.25 μL ddH2 O 1 μL Polymerase Chain Reaction:Article Title: A One-Step Process for the Construction of Phage Display scFv and VHH Libraries. Article Snippet: In this work we present a one-step cloning approach for the establishment of antibody phage display libraries relying on type IIs restriction enzymes. .. In this work we present a one-step cloning approach for the establishment of antibody phage display libraries relying on type IIs restriction enzymes. .. In this work we present a one-step cloning approach for the establishment of antibody phage display libraries relying on type IIs restriction enzymes. Purification:Article Title: A One-Step Process for the Construction of Phage Display scFv and VHH Libraries. Article Snippet: In this work we present a one-step cloning approach for the establishment of antibody phage display libraries relying on type IIs restriction enzymes. .. In this work we present a one-step cloning approach for the establishment of antibody phage display libraries relying on type IIs restriction enzymes. .. In this work we present a one-step cloning approach for the establishment of antibody phage display libraries relying on type IIs restriction enzymes. Article Title: A bispecific immunotweezer prevents soluble PrP oligomers and abolishes prion toxicity Article Snippet: Reaction mix was heated for 4 min at 95°C on a heating block ThermoStat (Eppendorf), then the heating block was turned off and the reaction was allowed to proceed for 30 min on the block and was then put at 4°C. .. Golden Gate assembly [ ] was used in order to clone the double-stranded DNA Oligomers into the MLM3636 plasmid, using the following reaction: 150 ng MLM3636 plasmid 1 μL double stranded oligomer ligation mix 2 μL T4 ligase buffer (New England Biolabs) 13.25 μL ddH2 O 1 μL Amplification:Article Title: A One-Step Process for the Construction of Phage Display scFv and VHH Libraries. Article Snippet: In this work we present a one-step cloning approach for the establishment of antibody phage display libraries relying on type IIs restriction enzymes. .. In this work we present a one-step cloning approach for the establishment of antibody phage display libraries relying on type IIs restriction enzymes. .. In this work we present a one-step cloning approach for the establishment of antibody phage display libraries relying on type IIs restriction enzymes. Sequencing:Article Title: A One-Step Process for the Construction of Phage Display scFv and VHH Libraries. Article Snippet: In this work we present a one-step cloning approach for the establishment of antibody phage display libraries relying on type IIs restriction enzymes. .. In this work we present a one-step cloning approach for the establishment of antibody phage display libraries relying on type IIs restriction enzymes. .. In this work we present a one-step cloning approach for the establishment of antibody phage display libraries relying on type IIs restriction enzymes. Expressing:Article Title: Versatile labeling and detection of endogenous proteins using tag-assisted split enzyme complementation Article Snippet: For generating landing pad vectors from expression vectors without the correct overhangs, an oligonucleotide stuffer was used to complete the overhangs. .. TA-splitHalo BFP plasmids were made in 10 μL reactions comprising 20 fmol Kanamycin ColE1 digested backbone, 40 fmol TA-splitHalo fusion expression vectors, 40 fmol PGK-mTagBFP2 expression vector, 10x T4 Ligase Buffer (NEB B0202S), Article Title: Versatile labeling and detection of endogenous proteins using tag-assisted split enzyme complementation Article Snippet: Expression vectors were generated in 10 μL reactions containing 20 fmol CDS backbone, 40 fmol of each part insert, 10x T4 Ligase Buffer, BsaI-HF v2.0 (NEB R3733S/L), and T7 DNA Ligase with the same cycling conditions as the part vectors. .. Landing pad (LP) vectors were generated similarly to part vectors in 10 μL reactions with 20 fmol MTK landing pad entry backbone (Addgene #123932), 40 fmol of each expression vector plasmids, 10x T4 Ligase Buffer (NEB B0202S), Generated:Article Title: Versatile labeling and detection of endogenous proteins using tag-assisted split enzyme complementation Article Snippet: Expression vectors were generated in 10 μL reactions containing 20 fmol CDS backbone, 40 fmol of each part insert, 10x T4 Ligase Buffer, BsaI-HF v2.0 (NEB R3733S/L), and T7 DNA Ligase with the same cycling conditions as the part vectors. .. Landing pad (LP) vectors were generated similarly to part vectors in 10 μL reactions with 20 fmol MTK landing pad entry backbone (Addgene #123932), 40 fmol of each expression vector plasmids, 10x T4 Ligase Buffer (NEB B0202S), Ligation:Article Title: A bispecific immunotweezer prevents soluble PrP oligomers and abolishes prion toxicity Article Snippet: Reaction mix was heated for 4 min at 95°C on a heating block ThermoStat (Eppendorf), then the heating block was turned off and the reaction was allowed to proceed for 30 min on the block and was then put at 4°C. .. Golden Gate assembly [ ] was used in order to clone the double-stranded DNA Oligomers into the MLM3636 plasmid, using the following reaction: 150 ng MLM3636 plasmid 1 μL double stranded oligomer ligation mix 2 μL T4 ligase buffer (New England Biolabs) 13.25 μL ddH2 O 1 μL Transformation Assay:Article Title: A bispecific immunotweezer prevents soluble PrP oligomers and abolishes prion toxicity Article Snippet: Reaction mix was heated for 4 min at 95°C on a heating block ThermoStat (Eppendorf), then the heating block was turned off and the reaction was allowed to proceed for 30 min on the block and was then put at 4°C. .. Golden Gate assembly [ ] was used in order to clone the double-stranded DNA Oligomers into the MLM3636 plasmid, using the following reaction: 150 ng MLM3636 plasmid 1 μL double stranded oligomer ligation mix 2 μL T4 ligase buffer (New England Biolabs) 13.25 μL ddH2 O 1 μL Concentration Assay:Article Title: Systematic Assembly of a Full-Length Infectious cDNA of Mouse Hepatitis Virus Strain A59 Article Snippet: A consensus sequence for each of the cloned fragments was determined, and a consensus clone was assembled by using restriction enzymes and standard recombinant DNA techniques. .. Each of the plasmids was grown to a high concentration, isolated, and digested, or double digested, with Isolation:Article Title: Systematic Assembly of a Full-Length Infectious cDNA of Mouse Hepatitis Virus Strain A59 Article Snippet: A consensus sequence for each of the cloned fragments was determined, and a consensus clone was assembled by using restriction enzymes and standard recombinant DNA techniques. .. Each of the plasmids was grown to a high concentration, isolated, and digested, or double digested, with End-sequence Profiling:Article Title: Systematic Assembly of a Full-Length Infectious cDNA of Mouse Hepatitis Virus Strain A59 Article Snippet: A consensus sequence for each of the cloned fragments was determined, and a consensus clone was assembled by using restriction enzymes and standard recombinant DNA techniques. .. Each of the plasmids was grown to a high concentration, isolated, and digested, or double digested, with |