nb bsm i  (New England Biolabs)


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  • 96
    Name:
    Nb BsmI
    Description:
    Nb BsmI 1 000 units
    Catalog Number:
    r0706l
    Price:
    70
    Size:
    1 000 units
    Category:
    Restriction Enzymes
    Buy from Supplier


    Structured Review

    New England Biolabs nb bsm i
    Nb BsmI
    Nb BsmI 1 000 units
    https://www.bioz.com/result/nb bsm i/product/New England Biolabs
    Average 96 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    nb bsm i - by Bioz Stars, 2020-07
    96/100 stars

    Images

    1) Product Images from "Measurements of DNA-loop formation via Cre-mediated recombination"

    Article Title: Measurements of DNA-loop formation via Cre-mediated recombination

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gks430

    Method for generating doubly labeled plasmid DNAs. ( A ) We engineered novel-plasmid DNA constructs in which pairs of tandem BbvC I and Bsm I restriction sites flank respective loxP sites. The minor distance between centers of the loxP sites is 65 bp. ( B ) Tandem nicks were generated at BbvC I and Bsm I sites by treatment with mutant endonucleases Nb.BbvCI and Nb.BsmI. ( C ) Fluorophore labels (donor in green; acceptor in red) were incorporated by displacing the single-stranded fragments released by the tandem nicks, which were subsequently sealed with T4 DNA ligase. ( D ) The covalently closed plasmid was separated from residual nicked DNA and linearized by treatment with Pst I.
    Figure Legend Snippet: Method for generating doubly labeled plasmid DNAs. ( A ) We engineered novel-plasmid DNA constructs in which pairs of tandem BbvC I and Bsm I restriction sites flank respective loxP sites. The minor distance between centers of the loxP sites is 65 bp. ( B ) Tandem nicks were generated at BbvC I and Bsm I sites by treatment with mutant endonucleases Nb.BbvCI and Nb.BsmI. ( C ) Fluorophore labels (donor in green; acceptor in red) were incorporated by displacing the single-stranded fragments released by the tandem nicks, which were subsequently sealed with T4 DNA ligase. ( D ) The covalently closed plasmid was separated from residual nicked DNA and linearized by treatment with Pst I.

    Techniques Used: Labeling, Plasmid Preparation, Construct, Generated, Mutagenesis

    2) Product Images from "Sensitive RNA detection by combining three-way junction formation and primer generation-rolling circle amplification"

    Article Title: Sensitive RNA detection by combining three-way junction formation and primer generation-rolling circle amplification

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkr909

    Confirmation of 3WJ primer extension and signal primer generation products. 3WJ primer P1, 3WJ template T1b and RNA50 were mixed in different combinations [( A) : P1/T1b/RNA50, ( B ): P1/T1b, ( C ): P1/RNA50 and ( D ): T1b/RNA50] and incubated to form a 3WJ structure. Signal generation reaction was conducted at 60°C for 0, 15 and 30 min by adding an enzyme mix [0.2 U Vent(exo-) DNA polymerase and 1 U Nb.BsmI] to the samples. The reaction products were analyzed on 15% denaturing polyacrylamide gel. P1* indicates a reaction product through primer extension of 3WJ primer and SP indicates signal primer generated from 3WJ structure. The numbers in parentheses indicate the length or expected length of each probe or reaction product. Lane M is 20–100 nt oligonucleotide marker.
    Figure Legend Snippet: Confirmation of 3WJ primer extension and signal primer generation products. 3WJ primer P1, 3WJ template T1b and RNA50 were mixed in different combinations [( A) : P1/T1b/RNA50, ( B ): P1/T1b, ( C ): P1/RNA50 and ( D ): T1b/RNA50] and incubated to form a 3WJ structure. Signal generation reaction was conducted at 60°C for 0, 15 and 30 min by adding an enzyme mix [0.2 U Vent(exo-) DNA polymerase and 1 U Nb.BsmI] to the samples. The reaction products were analyzed on 15% denaturing polyacrylamide gel. P1* indicates a reaction product through primer extension of 3WJ primer and SP indicates signal primer generated from 3WJ structure. The numbers in parentheses indicate the length or expected length of each probe or reaction product. Lane M is 20–100 nt oligonucleotide marker.

    Techniques Used: Incubation, Generated, Marker

    Related Articles

    Clone Assay:

    Article Title: EasyClone 2.0: expanded toolkit of integrative vectors for stable gene expression in industrial Saccharomyces cerevisiae strains
    Article Snippet: .. For cloning of particular genes, the vectors were digested with FastDigest AsiSI (Life Technologies) restriction endonuclease, nicked with Nb.BsmI (New England BioLabs) and assembled with PCR amplified genes and promoter(s) of choice by USER reaction and subsequent transformation into E. coli . .. Heterologous genes were either synthesized by GeneArt or amplified from the genomic DNA of S. cerevisiae CEN.PK113-7D or Pichia stipitis DSM-3651 (obtained from The Leibniz Institute DSMZ—German Collection of Microorganisms and Cell Cultures) (Supplementary material Table S2).

    Amplification:

    Article Title: EasyClone 2.0: expanded toolkit of integrative vectors for stable gene expression in industrial Saccharomyces cerevisiae strains
    Article Snippet: .. For cloning of particular genes, the vectors were digested with FastDigest AsiSI (Life Technologies) restriction endonuclease, nicked with Nb.BsmI (New England BioLabs) and assembled with PCR amplified genes and promoter(s) of choice by USER reaction and subsequent transformation into E. coli . .. Heterologous genes were either synthesized by GeneArt or amplified from the genomic DNA of S. cerevisiae CEN.PK113-7D or Pichia stipitis DSM-3651 (obtained from The Leibniz Institute DSMZ—German Collection of Microorganisms and Cell Cultures) (Supplementary material Table S2).

    Purification:

    Article Title: Separable functions of Tof1/Timeless in intra-S-checkpoint signalling, replisome stability and DNA topological stress
    Article Snippet: .. For plasmid DNA catenation analysis of pRS316, purified DNA was nicked with Nb.Bsm1 (New England Biolabs, R0706S) according to manufacturer’s instructions. .. For fork pausing analysis of pRS416-RFB, purified DNA was digested with BamHI-HF (New England Biolabs, R3136S) and SnaBI (New England Biolabs, R0130L) according to manufacturer’s instructions.

    Article Title: Measurements of DNA-loop formation via Cre-mediated recombination
    Article Snippet: .. The purified plasmid was treated with the site-specific nicking endonucleases Nb.BbvC I and Nb.Bsm I ( ) (New England Biolabs, Ipswich, MA, USA) at 37 and 65°C, respectively. ..

    Article Title: Folding DNA origami from a double-stranded source of scaffold
    Article Snippet: .. To avoid potential complications associated with the impossibility of complete separation between strands that are topologically interwound, the DNA was nicked at a single site with Nb.BsmI (New England Biolabs) and subsequently gel purified. ..

    Polymerase Chain Reaction:

    Article Title: EasyClone 2.0: expanded toolkit of integrative vectors for stable gene expression in industrial Saccharomyces cerevisiae strains
    Article Snippet: .. For cloning of particular genes, the vectors were digested with FastDigest AsiSI (Life Technologies) restriction endonuclease, nicked with Nb.BsmI (New England BioLabs) and assembled with PCR amplified genes and promoter(s) of choice by USER reaction and subsequent transformation into E. coli . .. Heterologous genes were either synthesized by GeneArt or amplified from the genomic DNA of S. cerevisiae CEN.PK113-7D or Pichia stipitis DSM-3651 (obtained from The Leibniz Institute DSMZ—German Collection of Microorganisms and Cell Cultures) (Supplementary material Table S2).

    Transformation Assay:

    Article Title: EasyClone 2.0: expanded toolkit of integrative vectors for stable gene expression in industrial Saccharomyces cerevisiae strains
    Article Snippet: .. For cloning of particular genes, the vectors were digested with FastDigest AsiSI (Life Technologies) restriction endonuclease, nicked with Nb.BsmI (New England BioLabs) and assembled with PCR amplified genes and promoter(s) of choice by USER reaction and subsequent transformation into E. coli . .. Heterologous genes were either synthesized by GeneArt or amplified from the genomic DNA of S. cerevisiae CEN.PK113-7D or Pichia stipitis DSM-3651 (obtained from The Leibniz Institute DSMZ—German Collection of Microorganisms and Cell Cultures) (Supplementary material Table S2).

    Plasmid Preparation:

    Article Title: RNA polymerase I passage through nucleosomes depends on its lobe binding subunits
    Article Snippet: .. Biotinylated oligos downstream of the tail in reverse direction (oligos 4018 and 4220 for 1 kb and 2 kb transcripts, respectively) and an oligo containing a Nb.BsmI (NEB) nicking site (oligo 4220a) were used to generate templates of 1 and 2 kb using plasmid 2316 as template. .. PCR products were cut with Nb.BsmI and heat inactivated at 80°C for 20 min. After 10 min, a competitor oligo 4220a with the same sequence as the 24 nt 3’overhang was added in excess to anneal with released cleaved 5’Nb.BsmI fragment.

    Article Title: Separable functions of Tof1/Timeless in intra-S-checkpoint signalling, replisome stability and DNA topological stress
    Article Snippet: .. For plasmid DNA catenation analysis of pRS316, purified DNA was nicked with Nb.Bsm1 (New England Biolabs, R0706S) according to manufacturer’s instructions. .. For fork pausing analysis of pRS416-RFB, purified DNA was digested with BamHI-HF (New England Biolabs, R3136S) and SnaBI (New England Biolabs, R0130L) according to manufacturer’s instructions.

    Article Title: Measurements of DNA-loop formation via Cre-mediated recombination
    Article Snippet: .. The purified plasmid was treated with the site-specific nicking endonucleases Nb.BbvC I and Nb.Bsm I ( ) (New England Biolabs, Ipswich, MA, USA) at 37 and 65°C, respectively. ..

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  • 96
    New England Biolabs nb bsm i
    Method for generating doubly labeled plasmid DNAs. ( A ) We engineered novel-plasmid DNA constructs in which pairs of tandem <t>BbvC</t> I and <t>Bsm</t> I restriction sites flank respective loxP sites. The minor distance between centers of the loxP sites is 65 bp. ( B ) Tandem nicks were generated at BbvC I and Bsm I sites by treatment with mutant endonucleases Nb.BbvCI and Nb.BsmI. ( C ) Fluorophore labels (donor in green; acceptor in red) were incorporated by displacing the single-stranded fragments released by the tandem nicks, which were subsequently sealed with T4 DNA ligase. ( D ) The covalently closed plasmid was separated from residual nicked DNA and linearized by treatment with Pst I.
    Nb Bsm I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 23 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nb bsm i/product/New England Biolabs
    Average 96 stars, based on 23 article reviews
    Price from $9.99 to $1999.99
    nb bsm i - by Bioz Stars, 2020-07
    96/100 stars
      Buy from Supplier

    Image Search Results


    Method for generating doubly labeled plasmid DNAs. ( A ) We engineered novel-plasmid DNA constructs in which pairs of tandem BbvC I and Bsm I restriction sites flank respective loxP sites. The minor distance between centers of the loxP sites is 65 bp. ( B ) Tandem nicks were generated at BbvC I and Bsm I sites by treatment with mutant endonucleases Nb.BbvCI and Nb.BsmI. ( C ) Fluorophore labels (donor in green; acceptor in red) were incorporated by displacing the single-stranded fragments released by the tandem nicks, which were subsequently sealed with T4 DNA ligase. ( D ) The covalently closed plasmid was separated from residual nicked DNA and linearized by treatment with Pst I.

    Journal: Nucleic Acids Research

    Article Title: Measurements of DNA-loop formation via Cre-mediated recombination

    doi: 10.1093/nar/gks430

    Figure Lengend Snippet: Method for generating doubly labeled plasmid DNAs. ( A ) We engineered novel-plasmid DNA constructs in which pairs of tandem BbvC I and Bsm I restriction sites flank respective loxP sites. The minor distance between centers of the loxP sites is 65 bp. ( B ) Tandem nicks were generated at BbvC I and Bsm I sites by treatment with mutant endonucleases Nb.BbvCI and Nb.BsmI. ( C ) Fluorophore labels (donor in green; acceptor in red) were incorporated by displacing the single-stranded fragments released by the tandem nicks, which were subsequently sealed with T4 DNA ligase. ( D ) The covalently closed plasmid was separated from residual nicked DNA and linearized by treatment with Pst I.

    Article Snippet: The purified plasmid was treated with the site-specific nicking endonucleases Nb.BbvC I and Nb.Bsm I ( ) (New England Biolabs, Ipswich, MA, USA) at 37 and 65°C, respectively.

    Techniques: Labeling, Plasmid Preparation, Construct, Generated, Mutagenesis

    Confirmation of 3WJ primer extension and signal primer generation products. 3WJ primer P1, 3WJ template T1b and RNA50 were mixed in different combinations [( A) : P1/T1b/RNA50, ( B ): P1/T1b, ( C ): P1/RNA50 and ( D ): T1b/RNA50] and incubated to form a 3WJ structure. Signal generation reaction was conducted at 60°C for 0, 15 and 30 min by adding an enzyme mix [0.2 U Vent(exo-) DNA polymerase and 1 U Nb.BsmI] to the samples. The reaction products were analyzed on 15% denaturing polyacrylamide gel. P1* indicates a reaction product through primer extension of 3WJ primer and SP indicates signal primer generated from 3WJ structure. The numbers in parentheses indicate the length or expected length of each probe or reaction product. Lane M is 20–100 nt oligonucleotide marker.

    Journal: Nucleic Acids Research

    Article Title: Sensitive RNA detection by combining three-way junction formation and primer generation-rolling circle amplification

    doi: 10.1093/nar/gkr909

    Figure Lengend Snippet: Confirmation of 3WJ primer extension and signal primer generation products. 3WJ primer P1, 3WJ template T1b and RNA50 were mixed in different combinations [( A) : P1/T1b/RNA50, ( B ): P1/T1b, ( C ): P1/RNA50 and ( D ): T1b/RNA50] and incubated to form a 3WJ structure. Signal generation reaction was conducted at 60°C for 0, 15 and 30 min by adding an enzyme mix [0.2 U Vent(exo-) DNA polymerase and 1 U Nb.BsmI] to the samples. The reaction products were analyzed on 15% denaturing polyacrylamide gel. P1* indicates a reaction product through primer extension of 3WJ primer and SP indicates signal primer generated from 3WJ structure. The numbers in parentheses indicate the length or expected length of each probe or reaction product. Lane M is 20–100 nt oligonucleotide marker.

    Article Snippet: Vent (exo-) DNA polymerase, Nb.BsmI and exonuclease III were purchased from New England Biolabs.

    Techniques: Incubation, Generated, Marker