bfuai restriction enzyme  (New England Biolabs)


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  • 96
    Name:
    BfuAI
    Description:
    BfuAI 1 250 units
    Catalog Number:
    r0701l
    Price:
    269
    Size:
    1 250 units
    Category:
    Restriction Enzymes
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    Structured Review

    New England Biolabs bfuai restriction enzyme
    BfuAI
    BfuAI 1 250 units
    https://www.bioz.com/result/bfuai restriction enzyme/product/New England Biolabs
    Average 96 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    bfuai restriction enzyme - by Bioz Stars, 2020-07
    96/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Pooled CRISPR screening at high sensitivity with an empirically designed sgRNA library
    Article Snippet: .. For cloning, the HD CRISPR vector was digested with BfuAI (NEB) and BsrGI-HF (NEB) overnight and dephosphorylated using CIP (NEB). .. The resulting linearized ∼7000 bp vector missing the eGFP stuffer was gel purified again using the NucleoSpin Gel and PCR Clean-up kit (Machery Nagel) and used for library cloning in a one step digestion-ligation-reaction ( ).

    Purification:

    Article Title: Target identification of small molecules using large-scale CRISPR-Cas mutagenesis scanning of essential genes
    Article Snippet: .. The purified PCR products were then subjected to restriction digestion (six parallel reactions) with BfuAI (NEB) overnight at 50 °C. .. After digestion, six ligation reactions containing 33 ng of digested sgRNA/crRNAs and 500 ng of the BfuAI and NsiI predigested pLCKO vector were performed overnight at 16 °C with T4 DNA ligase (NEB).

    Article Title: Genome-wide CRISPR Screens in Primary Human T Cells Reveal Key Regulators of Immune Function
    Article Snippet: .. This stuffer was excised using the BfuAI restriction enzyme (NEB, #R0701) and the linear backbone was gel purified (Zymo, #D4007). ..

    other:

    Article Title: O6-Methylguanine induces altered proteins at the level of transcription in human cells
    Article Snippet: Chemicals and biochemicals PspOMI, DpnI, BfuAI, NotI, SalI, T4 PNK, T4 DNA ligase and helper phage M13K07 were purchased from New England Biolabs (Waltham, MA, USA).

    CRISPR:

    Article Title: Pooled CRISPR screening at high sensitivity with an empirically designed sgRNA library
    Article Snippet: .. For cloning, the HD CRISPR vector was digested with BfuAI (NEB) and BsrGI-HF (NEB) overnight and dephosphorylated using CIP (NEB). .. The resulting linearized ∼7000 bp vector missing the eGFP stuffer was gel purified again using the NucleoSpin Gel and PCR Clean-up kit (Machery Nagel) and used for library cloning in a one step digestion-ligation-reaction ( ).

    Polymerase Chain Reaction:

    Article Title: Target identification of small molecules using large-scale CRISPR-Cas mutagenesis scanning of essential genes
    Article Snippet: .. The purified PCR products were then subjected to restriction digestion (six parallel reactions) with BfuAI (NEB) overnight at 50 °C. .. After digestion, six ligation reactions containing 33 ng of digested sgRNA/crRNAs and 500 ng of the BfuAI and NsiI predigested pLCKO vector were performed overnight at 16 °C with T4 DNA ligase (NEB).

    Plasmid Preparation:

    Article Title: Genome-wide Screens Identify Lineage- and Tumor Specific-Genes Modulating MHC-I and MHC-II Immunosurveillance in Human Lymphomas
    Article Snippet: .. Empty vector was prepared by digestion with BfuAI (New England Biolabs) at 50°C for 3 hours and heat inactivation at 65°C for 25 minutes. .. This was followed by dephosphorylation via Antarctic Phosphatase (New England Biolabs) for 1.5 hours at 37°C and heat inactivation for 4 minutes at 80°C.

    Article Title: Pooled CRISPR screening at high sensitivity with an empirically designed sgRNA library
    Article Snippet: .. For cloning, the HD CRISPR vector was digested with BfuAI (NEB) and BsrGI-HF (NEB) overnight and dephosphorylated using CIP (NEB). .. The resulting linearized ∼7000 bp vector missing the eGFP stuffer was gel purified again using the NucleoSpin Gel and PCR Clean-up kit (Machery Nagel) and used for library cloning in a one step digestion-ligation-reaction ( ).

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  • 96
    New England Biolabs bfuai restriction enzyme
    Bfuai Restriction Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bfuai restriction enzyme/product/New England Biolabs
    Average 96 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    bfuai restriction enzyme - by Bioz Stars, 2020-07
    96/100 stars
      Buy from Supplier

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