nb bsssi  (New England Biolabs)


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  • 95
    Name:
    Nb BssSI
    Description:
    Nb BssSI 1 000 units
    Catalog Number:
    r0681s
    Price:
    71
    Size:
    1 000 units
    Category:
    Restriction Enzymes
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    Structured Review

    New England Biolabs nb bsssi
    Nb BssSI
    Nb BssSI 1 000 units
    https://www.bioz.com/result/nb bsssi/product/New England Biolabs
    Average 95 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    nb bsssi - by Bioz Stars, 2020-07
    95/100 stars

    Images

    1) Product Images from "Next-generation mapping: a novel approach for detection of pathogenic structural variants with a potential utility in clinical diagnosis"

    Article Title: Next-generation mapping: a novel approach for detection of pathogenic structural variants with a potential utility in clinical diagnosis

    Journal: Genome Medicine

    doi: 10.1186/s13073-017-0479-0

    NGM identified a 5.1-Mbp inversion disrupting DMD. Top : X chromosome and Ref-Seq genes ( orange ) present in the magnified region. Visual representation of the inversion where the middle section of the reference ( blue ) and patient ( yellow ) maps have inverted alignments. The sample maps were generated using Nb.BssSI ( top ) and Nt.BspQI ( bottom ) endonucleases. Nicked sites are represented by red (Nb.BssSI) or black (Nt.BspQI) vertical lines in the middle reference and top/bottom sample maps
    Figure Legend Snippet: NGM identified a 5.1-Mbp inversion disrupting DMD. Top : X chromosome and Ref-Seq genes ( orange ) present in the magnified region. Visual representation of the inversion where the middle section of the reference ( blue ) and patient ( yellow ) maps have inverted alignments. The sample maps were generated using Nb.BssSI ( top ) and Nt.BspQI ( bottom ) endonucleases. Nicked sites are represented by red (Nb.BssSI) or black (Nt.BspQI) vertical lines in the middle reference and top/bottom sample maps

    Techniques Used: Generated

    Related Articles

    Flow Cytometry:

    Article Title: Next-generation mapping: a novel approach for detection of pathogenic structural variants with a potential utility in clinical diagnosis
    Article Snippet: .. Depending on the amount of coverage needed and the type of chip used, 300/600/900 ng of purified high molecular weight DNA was nicked with nicking endonucleases Nt.BspQI or Nb.BssSI (New England BioLabs/Bionano Genomics) in 10X Buffer 3 (Bionano Genomics) at 37 °C for 2 h. The nicked DNA was then labeled with 10X Labeling Mix containing fluorophore-labeled nucleotides using Taq polymerase (NEB) at 72 °C for 1 h before being repaired with Taq ligase (NEB) and IrysPrep Repair Mix, NAD+, and 10X Thermopol buffer at 37 °C for 30 min. DNA backbone was stained for visualization and size identification with IrysPrep DNA stain, 5X DTT, and 4X flow buffer overnight at 4 °C (Bionano Genomics). .. Labeled DNA was loaded on Irys chip and run for 24 h (Fig. ).

    Labeling:

    Article Title: Next-generation mapping: a novel approach for detection of pathogenic structural variants with a potential utility in clinical diagnosis
    Article Snippet: .. Depending on the amount of coverage needed and the type of chip used, 300/600/900 ng of purified high molecular weight DNA was nicked with nicking endonucleases Nt.BspQI or Nb.BssSI (New England BioLabs/Bionano Genomics) in 10X Buffer 3 (Bionano Genomics) at 37 °C for 2 h. The nicked DNA was then labeled with 10X Labeling Mix containing fluorophore-labeled nucleotides using Taq polymerase (NEB) at 72 °C for 1 h before being repaired with Taq ligase (NEB) and IrysPrep Repair Mix, NAD+, and 10X Thermopol buffer at 37 °C for 30 min. DNA backbone was stained for visualization and size identification with IrysPrep DNA stain, 5X DTT, and 4X flow buffer overnight at 4 °C (Bionano Genomics). .. Labeled DNA was loaded on Irys chip and run for 24 h (Fig. ).

    Purification:

    Article Title: SMRT long reads and Direct Label and Stain optical maps allow the generation of a high-quality genome assembly for the European barn swallow (Hirundo rustica rustica)
    Article Snippet: .. Then, 300 ng of purified gDNA was nicked with Nb.BssSI (New England BioLabs, cat. no. R0681S) in NEB Buffer 3. .. The nicked DNA was labeled with a fluorescent-dUTP nucleotide analog using Taq DNA polymerase (New England BioLabs, cat. no. M0267S).

    Article Title: Next-generation mapping: a novel approach for detection of pathogenic structural variants with a potential utility in clinical diagnosis
    Article Snippet: .. Depending on the amount of coverage needed and the type of chip used, 300/600/900 ng of purified high molecular weight DNA was nicked with nicking endonucleases Nt.BspQI or Nb.BssSI (New England BioLabs/Bionano Genomics) in 10X Buffer 3 (Bionano Genomics) at 37 °C for 2 h. The nicked DNA was then labeled with 10X Labeling Mix containing fluorophore-labeled nucleotides using Taq polymerase (NEB) at 72 °C for 1 h before being repaired with Taq ligase (NEB) and IrysPrep Repair Mix, NAD+, and 10X Thermopol buffer at 37 °C for 30 min. DNA backbone was stained for visualization and size identification with IrysPrep DNA stain, 5X DTT, and 4X flow buffer overnight at 4 °C (Bionano Genomics). .. Labeled DNA was loaded on Irys chip and run for 24 h (Fig. ).

    Modification:

    Article Title: SMRT long reads and Direct Label and Stain optical maps allow the generation of a high-quality genome assembly for the European barn swallow (Hirundo rustica rustica)
    Article Snippet: .. For Nb.BssSI the "aggressive" settings were used without modification. ..

    other:

    Article Title: SMRT long reads and Direct Label and Stain optical maps allow the generation of a high-quality genome assembly for the European barn swallow (Hirundo rustica rustica)
    Article Snippet: In the experiment with Nb.BssSI, molecule N50 was 0.1298 Mbp for molecules above 20 kbp and 0.2336 Mbp for molecules above 150 kbp, with an average label density of 11.8/100 kbp for molecules above 150 kbp.

    Sequencing:

    Article Title: The genome of cowpea (Vigna unguiculata [L.] Walp.)
    Article Snippet: .. The nicking endonucleases Nt.BspQ I and Nb.BssS I (New England BioLabs, Ipswich, MA, USA) were chosen to label DNA molecules at specific sequence motifs. .. The nicked DNA molecules were stained according to instructions of the IrysPrep Reagent Kit (Bionano Genomics) as per Luo et al . ( ).

    Article Title: Sequencing a Juglans regia × J. microcarpa hybrid yields high-quality genome assemblies of parental species
    Article Snippet: .. Based on the frequency of recognition sites in the genome sequence of J. regia cv Chandler , we selected nicking endonucleases Nt.Bsp QI and Nb.Bss SI (New England BioLabs, Ipswich MA USA) for DNA nicking. ..

    Staining:

    Article Title: Next-generation mapping: a novel approach for detection of pathogenic structural variants with a potential utility in clinical diagnosis
    Article Snippet: .. Depending on the amount of coverage needed and the type of chip used, 300/600/900 ng of purified high molecular weight DNA was nicked with nicking endonucleases Nt.BspQI or Nb.BssSI (New England BioLabs/Bionano Genomics) in 10X Buffer 3 (Bionano Genomics) at 37 °C for 2 h. The nicked DNA was then labeled with 10X Labeling Mix containing fluorophore-labeled nucleotides using Taq polymerase (NEB) at 72 °C for 1 h before being repaired with Taq ligase (NEB) and IrysPrep Repair Mix, NAD+, and 10X Thermopol buffer at 37 °C for 30 min. DNA backbone was stained for visualization and size identification with IrysPrep DNA stain, 5X DTT, and 4X flow buffer overnight at 4 °C (Bionano Genomics). .. Labeled DNA was loaded on Irys chip and run for 24 h (Fig. ).

    Chromatin Immunoprecipitation:

    Article Title: Next-generation mapping: a novel approach for detection of pathogenic structural variants with a potential utility in clinical diagnosis
    Article Snippet: .. Depending on the amount of coverage needed and the type of chip used, 300/600/900 ng of purified high molecular weight DNA was nicked with nicking endonucleases Nt.BspQI or Nb.BssSI (New England BioLabs/Bionano Genomics) in 10X Buffer 3 (Bionano Genomics) at 37 °C for 2 h. The nicked DNA was then labeled with 10X Labeling Mix containing fluorophore-labeled nucleotides using Taq polymerase (NEB) at 72 °C for 1 h before being repaired with Taq ligase (NEB) and IrysPrep Repair Mix, NAD+, and 10X Thermopol buffer at 37 °C for 30 min. DNA backbone was stained for visualization and size identification with IrysPrep DNA stain, 5X DTT, and 4X flow buffer overnight at 4 °C (Bionano Genomics). .. Labeled DNA was loaded on Irys chip and run for 24 h (Fig. ).

    Molecular Weight:

    Article Title: Next-generation mapping: a novel approach for detection of pathogenic structural variants with a potential utility in clinical diagnosis
    Article Snippet: .. Depending on the amount of coverage needed and the type of chip used, 300/600/900 ng of purified high molecular weight DNA was nicked with nicking endonucleases Nt.BspQI or Nb.BssSI (New England BioLabs/Bionano Genomics) in 10X Buffer 3 (Bionano Genomics) at 37 °C for 2 h. The nicked DNA was then labeled with 10X Labeling Mix containing fluorophore-labeled nucleotides using Taq polymerase (NEB) at 72 °C for 1 h before being repaired with Taq ligase (NEB) and IrysPrep Repair Mix, NAD+, and 10X Thermopol buffer at 37 °C for 30 min. DNA backbone was stained for visualization and size identification with IrysPrep DNA stain, 5X DTT, and 4X flow buffer overnight at 4 °C (Bionano Genomics). .. Labeled DNA was loaded on Irys chip and run for 24 h (Fig. ).

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  • 95
    New England Biolabs nb bsssi
    NGM identified a 5.1-Mbp inversion disrupting DMD. Top : X chromosome and Ref-Seq genes ( orange ) present in the magnified region. Visual representation of the inversion where the middle section of the reference ( blue ) and patient ( yellow ) maps have inverted alignments. The sample maps were generated using <t>Nb.BssSI</t> ( top ) and <t>Nt.BspQI</t> ( bottom ) endonucleases. Nicked sites are represented by red (Nb.BssSI) or black (Nt.BspQI) vertical lines in the middle reference and top/bottom sample maps
    Nb Bsssi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nb bsssi/product/New England Biolabs
    Average 95 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    nb bsssi - by Bioz Stars, 2020-07
    95/100 stars
      Buy from Supplier

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    NGM identified a 5.1-Mbp inversion disrupting DMD. Top : X chromosome and Ref-Seq genes ( orange ) present in the magnified region. Visual representation of the inversion where the middle section of the reference ( blue ) and patient ( yellow ) maps have inverted alignments. The sample maps were generated using Nb.BssSI ( top ) and Nt.BspQI ( bottom ) endonucleases. Nicked sites are represented by red (Nb.BssSI) or black (Nt.BspQI) vertical lines in the middle reference and top/bottom sample maps

    Journal: Genome Medicine

    Article Title: Next-generation mapping: a novel approach for detection of pathogenic structural variants with a potential utility in clinical diagnosis

    doi: 10.1186/s13073-017-0479-0

    Figure Lengend Snippet: NGM identified a 5.1-Mbp inversion disrupting DMD. Top : X chromosome and Ref-Seq genes ( orange ) present in the magnified region. Visual representation of the inversion where the middle section of the reference ( blue ) and patient ( yellow ) maps have inverted alignments. The sample maps were generated using Nb.BssSI ( top ) and Nt.BspQI ( bottom ) endonucleases. Nicked sites are represented by red (Nb.BssSI) or black (Nt.BspQI) vertical lines in the middle reference and top/bottom sample maps

    Article Snippet: Depending on the amount of coverage needed and the type of chip used, 300/600/900 ng of purified high molecular weight DNA was nicked with nicking endonucleases Nt.BspQI or Nb.BssSI (New England BioLabs/Bionano Genomics) in 10X Buffer 3 (Bionano Genomics) at 37 °C for 2 h. The nicked DNA was then labeled with 10X Labeling Mix containing fluorophore-labeled nucleotides using Taq polymerase (NEB) at 72 °C for 1 h before being repaired with Taq ligase (NEB) and IrysPrep Repair Mix, NAD+, and 10X Thermopol buffer at 37 °C for 30 min. DNA backbone was stained for visualization and size identification with IrysPrep DNA stain, 5X DTT, and 4X flow buffer overnight at 4 °C (Bionano Genomics).

    Techniques: Generated