zrai  (New England Biolabs)


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  • 92
    Name:
    ZraI
    Description:
    ZraI 1 000 units
    Catalog Number:
    r0659l
    Price:
    269
    Category:
    Restriction Enzymes
    Size:
    1 000 units
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    Structured Review

    New England Biolabs zrai
    ZraI
    ZraI 1 000 units
    https://www.bioz.com/result/zrai/product/New England Biolabs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    zrai - by Bioz Stars, 2021-03
    92/100 stars

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    Related Articles

    Introduce:

    Article Title: Small secreted proteins enable biofilm development in the cyanobacterium Synechococcus elongatus
    Article Snippet: .. Vectors used to introduce the WT and mutated complementation DNA fragments into NS1 were generated using the GeneArt® Seamless Cloning and Assembly Kit (Life Technologies) on PCR-generated cloning fragments and standardized devices (a chloramphenicol resistance device and a NS1 device with tetracycline resistance) derived via ZraI or Eco RV-HF (New England Biolabs) restriction digestion of CYANO-VECTOR donor plasmids as described by Taton et al . .. To improve the efficiency of cloning, the 6-kb WT DNA fragment was constructed from two 3-kb fragments amplified with the Q5® High-Fidelity DNA polymerase (New England Biolabs).

    Generated:

    Article Title: Small secreted proteins enable biofilm development in the cyanobacterium Synechococcus elongatus
    Article Snippet: .. Vectors used to introduce the WT and mutated complementation DNA fragments into NS1 were generated using the GeneArt® Seamless Cloning and Assembly Kit (Life Technologies) on PCR-generated cloning fragments and standardized devices (a chloramphenicol resistance device and a NS1 device with tetracycline resistance) derived via ZraI or Eco RV-HF (New England Biolabs) restriction digestion of CYANO-VECTOR donor plasmids as described by Taton et al . .. To improve the efficiency of cloning, the 6-kb WT DNA fragment was constructed from two 3-kb fragments amplified with the Q5® High-Fidelity DNA polymerase (New England Biolabs).

    Clone Assay:

    Article Title: Small secreted proteins enable biofilm development in the cyanobacterium Synechococcus elongatus
    Article Snippet: .. Vectors used to introduce the WT and mutated complementation DNA fragments into NS1 were generated using the GeneArt® Seamless Cloning and Assembly Kit (Life Technologies) on PCR-generated cloning fragments and standardized devices (a chloramphenicol resistance device and a NS1 device with tetracycline resistance) derived via ZraI or Eco RV-HF (New England Biolabs) restriction digestion of CYANO-VECTOR donor plasmids as described by Taton et al . .. To improve the efficiency of cloning, the 6-kb WT DNA fragment was constructed from two 3-kb fragments amplified with the Q5® High-Fidelity DNA polymerase (New England Biolabs).

    Polymerase Chain Reaction:

    Article Title: Small secreted proteins enable biofilm development in the cyanobacterium Synechococcus elongatus
    Article Snippet: .. Vectors used to introduce the WT and mutated complementation DNA fragments into NS1 were generated using the GeneArt® Seamless Cloning and Assembly Kit (Life Technologies) on PCR-generated cloning fragments and standardized devices (a chloramphenicol resistance device and a NS1 device with tetracycline resistance) derived via ZraI or Eco RV-HF (New England Biolabs) restriction digestion of CYANO-VECTOR donor plasmids as described by Taton et al . .. To improve the efficiency of cloning, the 6-kb WT DNA fragment was constructed from two 3-kb fragments amplified with the Q5® High-Fidelity DNA polymerase (New England Biolabs).

    Derivative Assay:

    Article Title: Small secreted proteins enable biofilm development in the cyanobacterium Synechococcus elongatus
    Article Snippet: .. Vectors used to introduce the WT and mutated complementation DNA fragments into NS1 were generated using the GeneArt® Seamless Cloning and Assembly Kit (Life Technologies) on PCR-generated cloning fragments and standardized devices (a chloramphenicol resistance device and a NS1 device with tetracycline resistance) derived via ZraI or Eco RV-HF (New England Biolabs) restriction digestion of CYANO-VECTOR donor plasmids as described by Taton et al . .. To improve the efficiency of cloning, the 6-kb WT DNA fragment was constructed from two 3-kb fragments amplified with the Q5® High-Fidelity DNA polymerase (New England Biolabs).

    Expressing:

    Article Title: Variation in Mutation Spectra Among CRISPR/Cas9 Mutagenized Poplars
    Article Snippet: Next, the promoter, sgRNA, and terminator cassettes (with one or two sgRNAs) in the modified AtU6-26SK vectors and the 35S-Cas9-SK vector were digested with HindIII (NEB) and ligated together using T4 ligase (NEB). .. Then, the plant expression vector pK2GW7 was digested with KpnI-HF (NEB) and ZraI (NEB). .. The entire piece containing the sgRNA expression cassette(s) and the Cas9 expression cassette in the modified 35S-Cas9-SK vector was digested with KpnI-HF (NEB) and SmaI (NEB) and ligated into the KpnI and ZraI sites in the already digested pK2GW7 using T4 ligase (NEB).

    Article Title: Variation in Mutation Spectra Among CRISPR/Cas9 Mutagenized Poplars
    Article Snippet: Then, the plant expression vector pK2GW7 was digested with KpnI-HF (NEB) and ZraI (NEB). .. The entire piece containing the sgRNA expression cassette(s) and the Cas9 expression cassette in the modified 35S-Cas9-SK vector was digested with KpnI-HF (NEB) and SmaI (NEB) and ligated into the KpnI and ZraI sites in the already digested pK2GW7 using T4 ligase (NEB). .. For the empty-vector control construct, the Cas9 cassette was digested using KpnI-HF (NEB) and SmaI (NEB) from the 35S-Cas9-SK vector and ligated into the pK2GW7 already digested with KpnI and ZraI with T4 ligase (NEB).

    Plasmid Preparation:

    Article Title: Variation in Mutation Spectra Among CRISPR/Cas9 Mutagenized Poplars
    Article Snippet: Next, the promoter, sgRNA, and terminator cassettes (with one or two sgRNAs) in the modified AtU6-26SK vectors and the 35S-Cas9-SK vector were digested with HindIII (NEB) and ligated together using T4 ligase (NEB). .. Then, the plant expression vector pK2GW7 was digested with KpnI-HF (NEB) and ZraI (NEB). .. The entire piece containing the sgRNA expression cassette(s) and the Cas9 expression cassette in the modified 35S-Cas9-SK vector was digested with KpnI-HF (NEB) and SmaI (NEB) and ligated into the KpnI and ZraI sites in the already digested pK2GW7 using T4 ligase (NEB).

    Article Title: Variation in Mutation Spectra Among CRISPR/Cas9 Mutagenized Poplars
    Article Snippet: The entire piece containing the sgRNA expression cassette(s) and the Cas9 expression cassette in the modified 35S-Cas9-SK vector was digested with KpnI-HF (NEB) and SmaI (NEB) and ligated into the KpnI and ZraI sites in the already digested pK2GW7 using T4 ligase (NEB). .. For the empty-vector control construct, the Cas9 cassette was digested using KpnI-HF (NEB) and SmaI (NEB) from the 35S-Cas9-SK vector and ligated into the pK2GW7 already digested with KpnI and ZraI with T4 ligase (NEB). ..

    Article Title: Genetic Transformation of a C. trachomatis Ocular Isolate With the Functional Tryptophan Synthase Operon Confers an Indole-Rescuable Phenotype
    Article Snippet: The 2.7 Kb trpRBA operon was isolated by gel extraction using the Wizard® SV Gel and PCR Clean-Up System (Promega). .. The pCDS5KO shuttle vector (Wang et al., ) was digested with ZraI (NEB). .. After dephosphorylation (as above), pCDS5KO was ligated with trpRBA (1:7 plasmid to insert ratio) and incubated overnight at 16°C, and heat inactivated at 70°C for 10 min prior to transformation into chemically competent DH5α E. coli , as described above.

    Article Title: Variation in Mutation Spectra Among CRISPR/Cas9 Mutagenized Poplars
    Article Snippet: Then, the plant expression vector pK2GW7 was digested with KpnI-HF (NEB) and ZraI (NEB). .. The entire piece containing the sgRNA expression cassette(s) and the Cas9 expression cassette in the modified 35S-Cas9-SK vector was digested with KpnI-HF (NEB) and SmaI (NEB) and ligated into the KpnI and ZraI sites in the already digested pK2GW7 using T4 ligase (NEB). .. For the empty-vector control construct, the Cas9 cassette was digested using KpnI-HF (NEB) and SmaI (NEB) from the 35S-Cas9-SK vector and ligated into the pK2GW7 already digested with KpnI and ZraI with T4 ligase (NEB).

    Construct:

    Article Title: Variation in Mutation Spectra Among CRISPR/Cas9 Mutagenized Poplars
    Article Snippet: The entire piece containing the sgRNA expression cassette(s) and the Cas9 expression cassette in the modified 35S-Cas9-SK vector was digested with KpnI-HF (NEB) and SmaI (NEB) and ligated into the KpnI and ZraI sites in the already digested pK2GW7 using T4 ligase (NEB). .. For the empty-vector control construct, the Cas9 cassette was digested using KpnI-HF (NEB) and SmaI (NEB) from the 35S-Cas9-SK vector and ligated into the pK2GW7 already digested with KpnI and ZraI with T4 ligase (NEB). ..

    Modification:

    Article Title: Variation in Mutation Spectra Among CRISPR/Cas9 Mutagenized Poplars
    Article Snippet: Then, the plant expression vector pK2GW7 was digested with KpnI-HF (NEB) and ZraI (NEB). .. The entire piece containing the sgRNA expression cassette(s) and the Cas9 expression cassette in the modified 35S-Cas9-SK vector was digested with KpnI-HF (NEB) and SmaI (NEB) and ligated into the KpnI and ZraI sites in the already digested pK2GW7 using T4 ligase (NEB). .. For the empty-vector control construct, the Cas9 cassette was digested using KpnI-HF (NEB) and SmaI (NEB) from the 35S-Cas9-SK vector and ligated into the pK2GW7 already digested with KpnI and ZraI with T4 ligase (NEB).

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  • Team
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  • 92
    New England Biolabs zrai
    Zrai, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/zrai/product/New England Biolabs
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    zrai - by Bioz Stars, 2021-03
    92/100 stars
      Buy from Supplier

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