psp xi  (New England Biolabs)


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  • 95
    Name:
    PspXI
    Description:
    PspXI 1 000 units
    Catalog Number:
    r0656l
    Price:
    290
    Size:
    1 000 units
    Category:
    Restriction Enzymes
    Buy from Supplier


    Structured Review

    New England Biolabs psp xi
    PspXI
    PspXI 1 000 units
    https://www.bioz.com/result/psp xi/product/New England Biolabs
    Average 95 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    psp xi - by Bioz Stars, 2020-07
    95/100 stars

    Images

    1) Product Images from "Extra-Chromosomal DNA Sequencing Reveals Episomal Prophages Capable of Impacting Virulence Factor Expression in Staphylococcus aureus"

    Article Title: Extra-Chromosomal DNA Sequencing Reveals Episomal Prophages Capable of Impacting Virulence Factor Expression in Staphylococcus aureus

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2018.01406

    Agarose gel of PCRs from ϕSa4ms selective-depletion experiment. One percent agarose gel stained with SYBR Safe DNA Gel Stain containing end-point PCRs from MSSA476 samples. MSSA476 exDNA was digested with Plasmid-safe exonuclease, Psh AI/ Psp XI restriction endonucleases, both Plasmid-safe and restriction endonuclease, or left untreated with treatment indicated by “+” and “–”. Gapdh , ϕSa3ms attP , ϕSa4ms attP , and pSAS1 target were amplified from treated and untreated samples. ϕSa4ms attP target is selectively depleted from combination exonuclease and endonuclease treatment but not solely exonuclease treatment, indicating its existence as a circular element in exDNA samples. Higher levels of ϕSa4ms target compared to ϕSa3ms target correlate with phage enrichment in sequencing. Negative controls for each primer set are shown. The 500 bp ladder band is indicated.
    Figure Legend Snippet: Agarose gel of PCRs from ϕSa4ms selective-depletion experiment. One percent agarose gel stained with SYBR Safe DNA Gel Stain containing end-point PCRs from MSSA476 samples. MSSA476 exDNA was digested with Plasmid-safe exonuclease, Psh AI/ Psp XI restriction endonucleases, both Plasmid-safe and restriction endonuclease, or left untreated with treatment indicated by “+” and “–”. Gapdh , ϕSa3ms attP , ϕSa4ms attP , and pSAS1 target were amplified from treated and untreated samples. ϕSa4ms attP target is selectively depleted from combination exonuclease and endonuclease treatment but not solely exonuclease treatment, indicating its existence as a circular element in exDNA samples. Higher levels of ϕSa4ms target compared to ϕSa3ms target correlate with phage enrichment in sequencing. Negative controls for each primer set are shown. The 500 bp ladder band is indicated.

    Techniques Used: Agarose Gel Electrophoresis, Staining, Plasmid Preparation, Amplification, Sequencing

    2) Product Images from "Extra-Chromosomal DNA Sequencing Reveals Episomal Prophages Capable of Impacting Virulence Factor Expression in Staphylococcus aureus"

    Article Title: Extra-Chromosomal DNA Sequencing Reveals Episomal Prophages Capable of Impacting Virulence Factor Expression in Staphylococcus aureus

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2018.01406

    Agarose gel of PCRs from ϕSa4ms selective-depletion experiment. One percent agarose gel stained with SYBR Safe DNA Gel Stain containing end-point PCRs from MSSA476 samples. MSSA476 exDNA was digested with Plasmid-safe exonuclease, Psh AI/ Psp XI restriction endonucleases, both Plasmid-safe and restriction endonuclease, or left untreated with treatment indicated by “+” and “–”. Gapdh , ϕSa3ms attP , ϕSa4ms attP , and pSAS1 target were amplified from treated and untreated samples. ϕSa4ms attP target is selectively depleted from combination exonuclease and endonuclease treatment but not solely exonuclease treatment, indicating its existence as a circular element in exDNA samples. Higher levels of ϕSa4ms target compared to ϕSa3ms target correlate with phage enrichment in sequencing. Negative controls for each primer set are shown. The 500 bp ladder band is indicated.
    Figure Legend Snippet: Agarose gel of PCRs from ϕSa4ms selective-depletion experiment. One percent agarose gel stained with SYBR Safe DNA Gel Stain containing end-point PCRs from MSSA476 samples. MSSA476 exDNA was digested with Plasmid-safe exonuclease, Psh AI/ Psp XI restriction endonucleases, both Plasmid-safe and restriction endonuclease, or left untreated with treatment indicated by “+” and “–”. Gapdh , ϕSa3ms attP , ϕSa4ms attP , and pSAS1 target were amplified from treated and untreated samples. ϕSa4ms attP target is selectively depleted from combination exonuclease and endonuclease treatment but not solely exonuclease treatment, indicating its existence as a circular element in exDNA samples. Higher levels of ϕSa4ms target compared to ϕSa3ms target correlate with phage enrichment in sequencing. Negative controls for each primer set are shown. The 500 bp ladder band is indicated.

    Techniques Used: Agarose Gel Electrophoresis, Staining, Plasmid Preparation, Amplification, Sequencing

    Related Articles

    Amplification:

    Article Title: Improving microbial fitness in the mammalian gut by in vivo temporal functional metagenomics
    Article Snippet: .. Isolated DNA was digested with PspXI and AvrII enzymes (New England Biolabs) prior to purification with QIAquick PCR Purification kit (Qiagen) and subsequent PCR amplification with primers A_L and A_R ( ) in KAPA HiFi HotStart Mix. .. The PCR was purified with AMPure beads at a beads:sample volumetric ratio of 0:5:1.

    Article Title: Improved Lentiviral Gene Delivery to Mouse Liver by Hydrodynamic Vector Injection through Tail Vein
    Article Snippet: .. The lentiviral vector pCCL-PGK-mPAH encoding the murine PAH (EC1.14.16.1) was generated from pCCL-PGK-fLuc by digestion with BamHI and PspXI (New England Biolabs) and insertion of murine PAH cDNA fragment (corresponding to GenBank UID X51942) amplified by RT-PCR from RNA isolated from murine fibroblasts using BamHI and PspXI -tagged primers. .. The HA-tag (MYPYDVPDYA) was fused N-terminally to the mPAH cDNA by QuikChangeII site-directed Mutagenesis Kit (Stratagene, Germany) to generate pCCL-PGK-HAtag-mPAH.

    Article Title: Chromosomal Complementation Using Tn7 Transposon Vectors in Enterobacteriaceae
    Article Snippet: .. The amplified product was digested with MfeI and PspxI (New England Biolabs) and then ligated into the suicide vector pGP704 previously digested with EcoRI and SalI, creating plasmid pGP-Tn 7 -Gm ( ). .. Second, the Tn 7 transposase-encoding genes tnsABCD were excised from plasmid pTNS2 by SphI and XmaI digestion and ligated into the same sites of the temperature-sensitive plasmid pST76-K, resulting in plasmid pSTNSK ( ).

    Mutagenesis:

    Article Title: Role of Dicer1 in thyroid cell proliferation and differentiation
    Article Snippet: .. The vector containing the c.5438A > G (E1813G) mutation was constructed by excising the 788 bp fragment, flanking the mutation site, using the restriction enzymes XmaI (#R0180S; New England BioLabs) and PspXI (#R0656L; New England BioLabs) and, further, inserting the synthetized fragment containing the c.5438A > G (E1813G) mutation (Integrated DNA Technologies) into the linearized pDICER1wt, generating a plasmid encoding human mutated DICER1 (pDICER1mut). .. The plasmid was sequenced (Eurofins Genomics) and DICER1 expression was validated by q-RT-PCR and western blot analysis.

    Isolation:

    Article Title: Improving microbial fitness in the mammalian gut by in vivo temporal functional metagenomics
    Article Snippet: .. Isolated DNA was digested with PspXI and AvrII enzymes (New England Biolabs) prior to purification with QIAquick PCR Purification kit (Qiagen) and subsequent PCR amplification with primers A_L and A_R ( ) in KAPA HiFi HotStart Mix. .. The PCR was purified with AMPure beads at a beads:sample volumetric ratio of 0:5:1.

    Article Title: Improved Lentiviral Gene Delivery to Mouse Liver by Hydrodynamic Vector Injection through Tail Vein
    Article Snippet: .. The lentiviral vector pCCL-PGK-mPAH encoding the murine PAH (EC1.14.16.1) was generated from pCCL-PGK-fLuc by digestion with BamHI and PspXI (New England Biolabs) and insertion of murine PAH cDNA fragment (corresponding to GenBank UID X51942) amplified by RT-PCR from RNA isolated from murine fibroblasts using BamHI and PspXI -tagged primers. .. The HA-tag (MYPYDVPDYA) was fused N-terminally to the mPAH cDNA by QuikChangeII site-directed Mutagenesis Kit (Stratagene, Germany) to generate pCCL-PGK-HAtag-mPAH.

    Construct:

    Article Title: Role of Dicer1 in thyroid cell proliferation and differentiation
    Article Snippet: .. The vector containing the c.5438A > G (E1813G) mutation was constructed by excising the 788 bp fragment, flanking the mutation site, using the restriction enzymes XmaI (#R0180S; New England BioLabs) and PspXI (#R0656L; New England BioLabs) and, further, inserting the synthetized fragment containing the c.5438A > G (E1813G) mutation (Integrated DNA Technologies) into the linearized pDICER1wt, generating a plasmid encoding human mutated DICER1 (pDICER1mut). .. The plasmid was sequenced (Eurofins Genomics) and DICER1 expression was validated by q-RT-PCR and western blot analysis.

    Article Title: The expression of Lamin A mutant R321X leads to endoplasmic reticulum stress with aberrant Ca2+ handling
    Article Snippet: .. For the generation of Lamin A m‐Cherry tagged constructs, EmGFP was excised from EmGFP‐tagged Lamin A constructs and substituted with mCherry excised from pMA‐RQ vector by XbaI and PspXI (both from New England Biolabs® Inc., Ipswich, MA, USA) double digestion. .. The final constructs were purified using QIAfilter™ Plasmid Maxi Kit (QIAgen, Valencia, CA, USA) and then verified by sequencing.

    Purification:

    Article Title: Improving microbial fitness in the mammalian gut by in vivo temporal functional metagenomics
    Article Snippet: .. Isolated DNA was digested with PspXI and AvrII enzymes (New England Biolabs) prior to purification with QIAquick PCR Purification kit (Qiagen) and subsequent PCR amplification with primers A_L and A_R ( ) in KAPA HiFi HotStart Mix. .. The PCR was purified with AMPure beads at a beads:sample volumetric ratio of 0:5:1.

    Polymerase Chain Reaction:

    Article Title: Improving microbial fitness in the mammalian gut by in vivo temporal functional metagenomics
    Article Snippet: .. Isolated DNA was digested with PspXI and AvrII enzymes (New England Biolabs) prior to purification with QIAquick PCR Purification kit (Qiagen) and subsequent PCR amplification with primers A_L and A_R ( ) in KAPA HiFi HotStart Mix. .. The PCR was purified with AMPure beads at a beads:sample volumetric ratio of 0:5:1.

    Generated:

    Article Title: Plant A20/AN1 protein serves as the important hub to mediate antiviral immunity
    Article Snippet: .. Fragments of different N-terminus deletions of Pha13 were generated with restriction enzyme digestions of FseI (NEB), ApaI (NEB), Sgr AI (NEB), BtgI (NEB), or PspXI (NEB). .. Fragments of C-terminus deletions were generated by PCR amplification using primer pairs, Nd13Fs412F/ Eco RI-Pha13-R, Nd13Ap361F/ Eco RI-Pha13-R, Nd13Sg247F/ Eco RI-Pha13-R, Nd13Bt136F /Eco RI-Pha13-R, and Nd13Ps88F /Eco RI-Pha13-R ( ).

    Article Title: Improved Lentiviral Gene Delivery to Mouse Liver by Hydrodynamic Vector Injection through Tail Vein
    Article Snippet: .. The lentiviral vector pCCL-PGK-mPAH encoding the murine PAH (EC1.14.16.1) was generated from pCCL-PGK-fLuc by digestion with BamHI and PspXI (New England Biolabs) and insertion of murine PAH cDNA fragment (corresponding to GenBank UID X51942) amplified by RT-PCR from RNA isolated from murine fibroblasts using BamHI and PspXI -tagged primers. .. The HA-tag (MYPYDVPDYA) was fused N-terminally to the mPAH cDNA by QuikChangeII site-directed Mutagenesis Kit (Stratagene, Germany) to generate pCCL-PGK-HAtag-mPAH.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Improved Lentiviral Gene Delivery to Mouse Liver by Hydrodynamic Vector Injection through Tail Vein
    Article Snippet: .. The lentiviral vector pCCL-PGK-mPAH encoding the murine PAH (EC1.14.16.1) was generated from pCCL-PGK-fLuc by digestion with BamHI and PspXI (New England Biolabs) and insertion of murine PAH cDNA fragment (corresponding to GenBank UID X51942) amplified by RT-PCR from RNA isolated from murine fibroblasts using BamHI and PspXI -tagged primers. .. The HA-tag (MYPYDVPDYA) was fused N-terminally to the mPAH cDNA by QuikChangeII site-directed Mutagenesis Kit (Stratagene, Germany) to generate pCCL-PGK-HAtag-mPAH.

    Plasmid Preparation:

    Article Title: Role of Dicer1 in thyroid cell proliferation and differentiation
    Article Snippet: .. The vector containing the c.5438A > G (E1813G) mutation was constructed by excising the 788 bp fragment, flanking the mutation site, using the restriction enzymes XmaI (#R0180S; New England BioLabs) and PspXI (#R0656L; New England BioLabs) and, further, inserting the synthetized fragment containing the c.5438A > G (E1813G) mutation (Integrated DNA Technologies) into the linearized pDICER1wt, generating a plasmid encoding human mutated DICER1 (pDICER1mut). .. The plasmid was sequenced (Eurofins Genomics) and DICER1 expression was validated by q-RT-PCR and western blot analysis.

    Article Title: Extra-Chromosomal DNA Sequencing Reveals Episomal Prophages Capable of Impacting Virulence Factor Expression in Staphylococcus aureus
    Article Snippet: .. PreCR-treated samples were then either (1) treated with the ϕSa4ms-specific restriction endonucleases Psh AI and Psp XI (NEB) following manufacturer’s protocol, (2) treated with Plasmid-Safe-ATP-Dependent DNase (Epicentre), (3) treated with Psh AI and Psp XI, then the Plasmid-Safe-ATP-Dependent DNase, or (4) left solely PreCR-treated. .. Plasmid-Safe treated samples were treated over the course of 16 h at 37°C, where 2 μL ATP solution and 2 μL DNase were added at 2 and 4 h time points.

    Article Title: Improved Lentiviral Gene Delivery to Mouse Liver by Hydrodynamic Vector Injection through Tail Vein
    Article Snippet: .. The lentiviral vector pCCL-PGK-mPAH encoding the murine PAH (EC1.14.16.1) was generated from pCCL-PGK-fLuc by digestion with BamHI and PspXI (New England Biolabs) and insertion of murine PAH cDNA fragment (corresponding to GenBank UID X51942) amplified by RT-PCR from RNA isolated from murine fibroblasts using BamHI and PspXI -tagged primers. .. The HA-tag (MYPYDVPDYA) was fused N-terminally to the mPAH cDNA by QuikChangeII site-directed Mutagenesis Kit (Stratagene, Germany) to generate pCCL-PGK-HAtag-mPAH.

    Article Title: The expression of Lamin A mutant R321X leads to endoplasmic reticulum stress with aberrant Ca2+ handling
    Article Snippet: .. For the generation of Lamin A m‐Cherry tagged constructs, EmGFP was excised from EmGFP‐tagged Lamin A constructs and substituted with mCherry excised from pMA‐RQ vector by XbaI and PspXI (both from New England Biolabs® Inc., Ipswich, MA, USA) double digestion. .. The final constructs were purified using QIAfilter™ Plasmid Maxi Kit (QIAgen, Valencia, CA, USA) and then verified by sequencing.

    Article Title: Chromosomal Complementation Using Tn7 Transposon Vectors in Enterobacteriaceae
    Article Snippet: .. The amplified product was digested with MfeI and PspxI (New England Biolabs) and then ligated into the suicide vector pGP704 previously digested with EcoRI and SalI, creating plasmid pGP-Tn 7 -Gm ( ). .. Second, the Tn 7 transposase-encoding genes tnsABCD were excised from plasmid pTNS2 by SphI and XmaI digestion and ligated into the same sites of the temperature-sensitive plasmid pST76-K, resulting in plasmid pSTNSK ( ).

    Article Title: Extra-Chromosomal DNA Sequencing Reveals Episomal Prophages Capable of Impacting Virulence Factor Expression in Staphylococcus aureus
    Article Snippet: .. PreCR-treated samples were then either (1) treated with the ϕSa4ms-specific restriction endonucleases Psh AI and Psp XI (NEB) following manufacturer’s protocol, (2) treated with Plasmid-Safe-ATP-Dependent DNase (Epicentre), (3) treated with Psh AI and Psp XI, then the Plasmid-Safe-ATP-Dependent DNase, or (4) left solely PreCR-treated. .. Plasmid-Safe treated samples were treated over the course of 16 h at 37°C, where 2 μL ATP solution and 2 μL DNase were added at 2 and 4 h time points.

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  • 95
    New England Biolabs psp xi
    Agarose gel of PCRs from <t>ϕSa4ms</t> selective-depletion experiment. One percent agarose gel stained with SYBR Safe DNA Gel Stain containing end-point PCRs from MSSA476 samples. MSSA476 exDNA was digested with Plasmid-safe exonuclease, Psh AI/ <t>Psp</t> XI restriction endonucleases, both Plasmid-safe and restriction endonuclease, or left untreated with treatment indicated by “+” and “–”. Gapdh , ϕSa3ms attP , ϕSa4ms attP , and pSAS1 target were amplified from treated and untreated samples. ϕSa4ms attP target is selectively depleted from combination exonuclease and endonuclease treatment but not solely exonuclease treatment, indicating its existence as a circular element in exDNA samples. Higher levels of ϕSa4ms target compared to ϕSa3ms target correlate with phage enrichment in sequencing. Negative controls for each primer set are shown. The 500 bp ladder band is indicated.
    Psp Xi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 13 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/psp xi/product/New England Biolabs
    Average 95 stars, based on 13 article reviews
    Price from $9.99 to $1999.99
    psp xi - by Bioz Stars, 2020-07
    95/100 stars
      Buy from Supplier

    Image Search Results


    Agarose gel of PCRs from ϕSa4ms selective-depletion experiment. One percent agarose gel stained with SYBR Safe DNA Gel Stain containing end-point PCRs from MSSA476 samples. MSSA476 exDNA was digested with Plasmid-safe exonuclease, Psh AI/ Psp XI restriction endonucleases, both Plasmid-safe and restriction endonuclease, or left untreated with treatment indicated by “+” and “–”. Gapdh , ϕSa3ms attP , ϕSa4ms attP , and pSAS1 target were amplified from treated and untreated samples. ϕSa4ms attP target is selectively depleted from combination exonuclease and endonuclease treatment but not solely exonuclease treatment, indicating its existence as a circular element in exDNA samples. Higher levels of ϕSa4ms target compared to ϕSa3ms target correlate with phage enrichment in sequencing. Negative controls for each primer set are shown. The 500 bp ladder band is indicated.

    Journal: Frontiers in Microbiology

    Article Title: Extra-Chromosomal DNA Sequencing Reveals Episomal Prophages Capable of Impacting Virulence Factor Expression in Staphylococcus aureus

    doi: 10.3389/fmicb.2018.01406

    Figure Lengend Snippet: Agarose gel of PCRs from ϕSa4ms selective-depletion experiment. One percent agarose gel stained with SYBR Safe DNA Gel Stain containing end-point PCRs from MSSA476 samples. MSSA476 exDNA was digested with Plasmid-safe exonuclease, Psh AI/ Psp XI restriction endonucleases, both Plasmid-safe and restriction endonuclease, or left untreated with treatment indicated by “+” and “–”. Gapdh , ϕSa3ms attP , ϕSa4ms attP , and pSAS1 target were amplified from treated and untreated samples. ϕSa4ms attP target is selectively depleted from combination exonuclease and endonuclease treatment but not solely exonuclease treatment, indicating its existence as a circular element in exDNA samples. Higher levels of ϕSa4ms target compared to ϕSa3ms target correlate with phage enrichment in sequencing. Negative controls for each primer set are shown. The 500 bp ladder band is indicated.

    Article Snippet: PreCR-treated samples were then either (1) treated with the ϕSa4ms-specific restriction endonucleases Psh AI and Psp XI (NEB) following manufacturer’s protocol, (2) treated with Plasmid-Safe-ATP-Dependent DNase (Epicentre), (3) treated with Psh AI and Psp XI, then the Plasmid-Safe-ATP-Dependent DNase, or (4) left solely PreCR-treated.

    Techniques: Agarose Gel Electrophoresis, Staining, Plasmid Preparation, Amplification, Sequencing

    Agarose gel of PCRs from ϕSa4ms selective-depletion experiment. One percent agarose gel stained with SYBR Safe DNA Gel Stain containing end-point PCRs from MSSA476 samples. MSSA476 exDNA was digested with Plasmid-safe exonuclease, Psh AI/ Psp XI restriction endonucleases, both Plasmid-safe and restriction endonuclease, or left untreated with treatment indicated by “+” and “–”. Gapdh , ϕSa3ms attP , ϕSa4ms attP , and pSAS1 target were amplified from treated and untreated samples. ϕSa4ms attP target is selectively depleted from combination exonuclease and endonuclease treatment but not solely exonuclease treatment, indicating its existence as a circular element in exDNA samples. Higher levels of ϕSa4ms target compared to ϕSa3ms target correlate with phage enrichment in sequencing. Negative controls for each primer set are shown. The 500 bp ladder band is indicated.

    Journal: Frontiers in Microbiology

    Article Title: Extra-Chromosomal DNA Sequencing Reveals Episomal Prophages Capable of Impacting Virulence Factor Expression in Staphylococcus aureus

    doi: 10.3389/fmicb.2018.01406

    Figure Lengend Snippet: Agarose gel of PCRs from ϕSa4ms selective-depletion experiment. One percent agarose gel stained with SYBR Safe DNA Gel Stain containing end-point PCRs from MSSA476 samples. MSSA476 exDNA was digested with Plasmid-safe exonuclease, Psh AI/ Psp XI restriction endonucleases, both Plasmid-safe and restriction endonuclease, or left untreated with treatment indicated by “+” and “–”. Gapdh , ϕSa3ms attP , ϕSa4ms attP , and pSAS1 target were amplified from treated and untreated samples. ϕSa4ms attP target is selectively depleted from combination exonuclease and endonuclease treatment but not solely exonuclease treatment, indicating its existence as a circular element in exDNA samples. Higher levels of ϕSa4ms target compared to ϕSa3ms target correlate with phage enrichment in sequencing. Negative controls for each primer set are shown. The 500 bp ladder band is indicated.

    Article Snippet: PreCR-treated samples were then either (1) treated with the ϕSa4ms-specific restriction endonucleases Psh AI and Psp XI (NEB) following manufacturer’s protocol, (2) treated with Plasmid-Safe-ATP-Dependent DNase (Epicentre), (3) treated with Psh AI and Psp XI, then the Plasmid-Safe-ATP-Dependent DNase, or (4) left solely PreCR-treated.

    Techniques: Agarose Gel Electrophoresis, Staining, Plasmid Preparation, Amplification, Sequencing