bstapi  (New England Biolabs)


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  • 93
    Name:
    BstAPI
    Description:
    BstAPI 1 000 units
    Catalog Number:
    r0654l
    Price:
    382
    Size:
    1 000 units
    Category:
    Restriction Enzymes
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    Structured Review

    New England Biolabs bstapi
    BstAPI
    BstAPI 1 000 units
    https://www.bioz.com/result/bstapi/product/New England Biolabs
    Average 93 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    bstapi - by Bioz Stars, 2020-09
    93/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: A PCR-mutagenesis strategy for rapid detection of mutations in codon 634 of the ret proto-oncogene related to MEN 2A.
    Article Snippet: .. PCR products were incubated with 0.5 μl of Bst ApI (10 U/μl New England Biolabs, Buenos Aires, Argentina) or Bgl I (10 U/μl Promega, Buenos Aires, Argentina), in a 25 μl reaction volume. .. The same reaction replacing the enzyme with miliQwater was used as negative control.

    Incubation:

    Article Title: Systematic Assembly of a Full-Length Infectious Clone of Human Coronavirus NL63 ▿Systematic Assembly of a Full-Length Infectious Clone of Human Coronavirus NL63 ▿ †
    Article Snippet: .. Briefly, 25 μl of DNA for each virus was incubated with 1 μl BstAPI, 3 μl NEB (New England Biolabs) buffer 3, and 1 μl double-distilled water at 60°C for 2 h and then electroporated on a 0.8% agarose gel. .. The remaining 5 μl of DNA was used to sequence the fragment for genotype verification.

    Article Title: A PCR-mutagenesis strategy for rapid detection of mutations in codon 634 of the ret proto-oncogene related to MEN 2A.
    Article Snippet: .. PCR products were incubated with 0.5 μl of Bst ApI (10 U/μl New England Biolabs, Buenos Aires, Argentina) or Bgl I (10 U/μl Promega, Buenos Aires, Argentina), in a 25 μl reaction volume. .. The same reaction replacing the enzyme with miliQwater was used as negative control.

    Agarose Gel Electrophoresis:

    Article Title: Systematic Assembly of a Full-Length Infectious Clone of Human Coronavirus NL63 ▿Systematic Assembly of a Full-Length Infectious Clone of Human Coronavirus NL63 ▿ †
    Article Snippet: .. Briefly, 25 μl of DNA for each virus was incubated with 1 μl BstAPI, 3 μl NEB (New England Biolabs) buffer 3, and 1 μl double-distilled water at 60°C for 2 h and then electroporated on a 0.8% agarose gel. .. The remaining 5 μl of DNA was used to sequence the fragment for genotype verification.

    Subcloning:

    Article Title: TMC1 Forms the Pore of Mechanosensory Transduction Channels in Vertebrate Inner Ear Hair Cells
    Article Snippet: .. For cysteine changes between amino acid positions 393 and 535, flanking restriction sites for AvrII and BstAPI (all enzymes were purchased from New England Biolabs Inc., Ipswich, MA) were added to the ends to permit subcloning into pAAV2-CBA- Tmc1ex1 vector. .. For amino acid positions between 567 and 596, BastAPI and ApaI enzymes were used for subcloning.

    Plasmid Preparation:

    Article Title: TMC1 Forms the Pore of Mechanosensory Transduction Channels in Vertebrate Inner Ear Hair Cells
    Article Snippet: .. For cysteine changes between amino acid positions 393 and 535, flanking restriction sites for AvrII and BstAPI (all enzymes were purchased from New England Biolabs Inc., Ipswich, MA) were added to the ends to permit subcloning into pAAV2-CBA- Tmc1ex1 vector. .. For amino acid positions between 567 and 596, BastAPI and ApaI enzymes were used for subcloning.

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  • 93
    New England Biolabs bstapi
    Bstapi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bstapi/product/New England Biolabs
    Average 93 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    bstapi - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

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