faui  (New England Biolabs)


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  • 93
    Name:
    FauI
    Description:
    FauI 1 000 units
    Catalog Number:
    R0651L
    Price:
    290
    Category:
    Restriction Enzymes
    Size:
    1 000 units
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    Structured Review

    New England Biolabs faui
    FauI
    FauI 1 000 units
    https://www.bioz.com/result/faui/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    faui - by Bioz Stars, 2021-04
    93/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: An Epidemiological Survey of Opisthorchis viverrini Infection in a Lightly Infected Community, Eastern Thailand
    Article Snippet: .. The 375 bp and 381 bp of PCR products were subjected to PCR-RFLP to discriminate eggs of O. viverrini and C. sinensis using a restriction enzyme that digested the PCR amplicons with 2 units of Fau I (New England Biolabs, Ipswich, MA) in a total volume of 20 μL at 55°C overnight. ..

    Article Title: Impaired labyrinth formation prevents the establishment of the maternal-fetal interface in conditional Hand1-deficient mice
    Article Snippet: .. To detect the frameshift mutation at the Hand1 locus, the Hand1 PCR product was digested with the restriction enzyme FauI (New England Biolabs R0651) using 1 ul of restriction enzyme with 1.5 ul of PCR product, 2 ul CutSmart buffer, and 20ul RNAse-free water with the standard protocol, then run on an agarose gel. .. RNA expression via reverse transcription quantitative polymerase chain reaction (qPCR) Half placentas (E10.5 to E1.5) were flash frozen in liquid nitrogen and stored at −80C.

    Article Title: Distribution of the Warmblood Fragile Foal Syndrome Type 1 Mutation (PLOD1 c.2032G > A) in Different Horse Breeds from Europe and the United States
    Article Snippet: PCR was performed using AmpliTaq Gold™ 360 Master Mix (Thermo Fisher Scientific, Waltham, MA, USA) according to the protocol with an annealing temperature of 57 °C. .. PCR fragments were digested with FauI (New England Biolabs, Ipswich, MA, USA) according to the protocol and visualized on a 4% agarose gel (G-Allele 102 and 64 bp; A-Allele 166 bp). .. Amplicons from all heterozygous and some homozygous samples were confirmed by Sanger sequencing using BigDye™ Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific) and 3500×L Genetic Analyzers (Applied Biosystems, Foster, CA, USA; Thermo Fisher Scientific).

    Article Title: Prognostic Role of Host Cyclooxygenase and Cytokine Genotypes in a Caucasian Cohort of Patients with Gastric Adenocarcinoma
    Article Snippet: The fragment containing the PTGS1 +50C > T (Pro17Leu) (rs3842787) polymorphic site was amplified as described by Gonzalez-Conejero et al. . .. PCR products were digested with 2 units of Fau I (New England Biolabs, Izasa, Barcelona, Spain) for 5 hours at 55°C and electrophoresed on 2% agarose gels. ..

    Mutagenesis:

    Article Title: Impaired labyrinth formation prevents the establishment of the maternal-fetal interface in conditional Hand1-deficient mice
    Article Snippet: .. To detect the frameshift mutation at the Hand1 locus, the Hand1 PCR product was digested with the restriction enzyme FauI (New England Biolabs R0651) using 1 ul of restriction enzyme with 1.5 ul of PCR product, 2 ul CutSmart buffer, and 20ul RNAse-free water with the standard protocol, then run on an agarose gel. .. RNA expression via reverse transcription quantitative polymerase chain reaction (qPCR) Half placentas (E10.5 to E1.5) were flash frozen in liquid nitrogen and stored at −80C.

    Agarose Gel Electrophoresis:

    Article Title: Impaired labyrinth formation prevents the establishment of the maternal-fetal interface in conditional Hand1-deficient mice
    Article Snippet: .. To detect the frameshift mutation at the Hand1 locus, the Hand1 PCR product was digested with the restriction enzyme FauI (New England Biolabs R0651) using 1 ul of restriction enzyme with 1.5 ul of PCR product, 2 ul CutSmart buffer, and 20ul RNAse-free water with the standard protocol, then run on an agarose gel. .. RNA expression via reverse transcription quantitative polymerase chain reaction (qPCR) Half placentas (E10.5 to E1.5) were flash frozen in liquid nitrogen and stored at −80C.

    Article Title: Distribution of the Warmblood Fragile Foal Syndrome Type 1 Mutation (PLOD1 c.2032G > A) in Different Horse Breeds from Europe and the United States
    Article Snippet: PCR was performed using AmpliTaq Gold™ 360 Master Mix (Thermo Fisher Scientific, Waltham, MA, USA) according to the protocol with an annealing temperature of 57 °C. .. PCR fragments were digested with FauI (New England Biolabs, Ipswich, MA, USA) according to the protocol and visualized on a 4% agarose gel (G-Allele 102 and 64 bp; A-Allele 166 bp). .. Amplicons from all heterozygous and some homozygous samples were confirmed by Sanger sequencing using BigDye™ Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific) and 3500×L Genetic Analyzers (Applied Biosystems, Foster, CA, USA; Thermo Fisher Scientific).

    Real-time Polymerase Chain Reaction:

    Article Title: Epigenetic Regulation of Glucose Transporter 4 by Estrogen Receptor ?
    Article Snippet: Bias of the PCR primers and pyrosequencing mismatches were tested using 5-AZA-dC-treated genomic DNA from MEFs and Universal Methylated Mouse DNA (Zymo Research) in different ratios (Supplemental Fig. 1A). .. Genomic DNA (1 μg) from wt, erko, and βerko MEFs was subjected to Fau I (New England Biolabs, Inc., Beverly, MA) digest for 1 h at 55 C. Subsequently, 1/20 of this reaction was used in a real-time PCR amplifying Glut4 promoter. ..

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  • Bioz Stars
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  • 93
    New England Biolabs faui
    Faui, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/faui/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    faui - by Bioz Stars, 2021-04
    93/100 stars
      Buy from Supplier

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