fati  (New England Biolabs)


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  • 91
    Name:
    FatI
    Description:

    Catalog Number:
    R0650
    Price:
    389
    Category:
    Restriction Enzymes
    Applications:
    DNA Manipulation
    Size:
    250 units
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    Structured Review

    New England Biolabs fati
    FatI

    https://www.bioz.com/result/fati/product/New England Biolabs
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fati - by Bioz Stars, 2021-08
    91/100 stars

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    1) Product Images from "High-throughput sequencing of sorted expression libraries reveals inhibitors of bacterial cell division"

    Article Title: High-throughput sequencing of sorted expression libraries reveals inhibitors of bacterial cell division

    Journal: BMC Genomics

    doi: 10.1186/s12864-018-5187-7

    Partial fragments of ORFs cause filamentation. FatI fragment sub-clones from the ybiY-fsaA-moeB-moeA and ybeM-tatE-lipA regions were screened (48 colonies each) for filamentation after growth and induction for 7 h at 37 °C. The indicated clones were identified as causing filamentation. a BW25113/pBAD24 control, ( b ) Clone H8 ( ybeM ), ( c ) Clone A2 ( ybeM ), ( d ) Clone H9 ( ybeM ), ( e ) Clone C2 ( ybeM ), ( f ) Clone D10 ( ybeM ), ( g ) Clone C1 ( ybiY ), ( h ) Clone A4 ( ybiY ), ( i ) Clone A11 ( ybiY-moeA ). j The ybeM-tatE-lipA region and sub-clones identified as capable of causing filamentation; three fragments throughout the 7 clones, of 112 bp, 87 bp and 29 bp were identified within the ybeM gene. k Map of fragments within the ybiY-fsaA-moeB-moeA locus capable of causing filamentation. Some of the fragments indicated in ( j ) and ( k ) were isolated in multiple clones (see Table 3).indicated in parentheses). The asterisk indicates that the expressed fragments in these clones were in the opposite orientation in pBAD24 relative to the expected wild-type transcription of the indicated genes in UTI89. [The A11* clone was orientated with respect to pBAD in the order ybiY (partial) - fsaA - moeB – moeA (partial)]
    Figure Legend Snippet: Partial fragments of ORFs cause filamentation. FatI fragment sub-clones from the ybiY-fsaA-moeB-moeA and ybeM-tatE-lipA regions were screened (48 colonies each) for filamentation after growth and induction for 7 h at 37 °C. The indicated clones were identified as causing filamentation. a BW25113/pBAD24 control, ( b ) Clone H8 ( ybeM ), ( c ) Clone A2 ( ybeM ), ( d ) Clone H9 ( ybeM ), ( e ) Clone C2 ( ybeM ), ( f ) Clone D10 ( ybeM ), ( g ) Clone C1 ( ybiY ), ( h ) Clone A4 ( ybiY ), ( i ) Clone A11 ( ybiY-moeA ). j The ybeM-tatE-lipA region and sub-clones identified as capable of causing filamentation; three fragments throughout the 7 clones, of 112 bp, 87 bp and 29 bp were identified within the ybeM gene. k Map of fragments within the ybiY-fsaA-moeB-moeA locus capable of causing filamentation. Some of the fragments indicated in ( j ) and ( k ) were isolated in multiple clones (see Table 3).indicated in parentheses). The asterisk indicates that the expressed fragments in these clones were in the opposite orientation in pBAD24 relative to the expected wild-type transcription of the indicated genes in UTI89. [The A11* clone was orientated with respect to pBAD in the order ybiY (partial) - fsaA - moeB – moeA (partial)]

    Techniques Used: Clone Assay, Isolation

    2) Product Images from "High-throughput sequencing of sorted expression libraries reveals inhibitors of bacterial cell division"

    Article Title: High-throughput sequencing of sorted expression libraries reveals inhibitors of bacterial cell division

    Journal: BMC Genomics

    doi: 10.1186/s12864-018-5187-7

    Partial fragments of ORFs cause filamentation. FatI fragment sub-clones from the ybiY-fsaA-moeB-moeA and ybeM-tatE-lipA regions were screened (48 colonies each) for filamentation after growth and induction for 7 h at 37 °C. The indicated clones were identified as causing filamentation. a BW25113/pBAD24 control, ( b ) Clone H8 ( ybeM ), ( c ) Clone A2 ( ybeM ), ( d ) Clone H9 ( ybeM ), ( e ) Clone C2 ( ybeM ), ( f ) Clone D10 ( ybeM ), ( g ) Clone C1 ( ybiY ), ( h ) Clone A4 ( ybiY ), ( i ) Clone A11 ( ybiY-moeA ). j The ybeM-tatE-lipA region and sub-clones identified as capable of causing filamentation; three fragments throughout the 7 clones, of 112 bp, 87 bp and 29 bp were identified within the ybeM gene. k Map of fragments within the ybiY-fsaA-moeB-moeA locus capable of causing filamentation. Some of the fragments indicated in ( j ) and ( k ) were isolated in multiple clones (see Table 3).indicated in parentheses). The asterisk indicates that the expressed fragments in these clones were in the opposite orientation in pBAD24 relative to the expected wild-type transcription of the indicated genes in UTI89. [The A11* clone was orientated with respect to pBAD in the order ybiY (partial) - fsaA - moeB – moeA (partial)]
    Figure Legend Snippet: Partial fragments of ORFs cause filamentation. FatI fragment sub-clones from the ybiY-fsaA-moeB-moeA and ybeM-tatE-lipA regions were screened (48 colonies each) for filamentation after growth and induction for 7 h at 37 °C. The indicated clones were identified as causing filamentation. a BW25113/pBAD24 control, ( b ) Clone H8 ( ybeM ), ( c ) Clone A2 ( ybeM ), ( d ) Clone H9 ( ybeM ), ( e ) Clone C2 ( ybeM ), ( f ) Clone D10 ( ybeM ), ( g ) Clone C1 ( ybiY ), ( h ) Clone A4 ( ybiY ), ( i ) Clone A11 ( ybiY-moeA ). j The ybeM-tatE-lipA region and sub-clones identified as capable of causing filamentation; three fragments throughout the 7 clones, of 112 bp, 87 bp and 29 bp were identified within the ybeM gene. k Map of fragments within the ybiY-fsaA-moeB-moeA locus capable of causing filamentation. Some of the fragments indicated in ( j ) and ( k ) were isolated in multiple clones (see Table 3).indicated in parentheses). The asterisk indicates that the expressed fragments in these clones were in the opposite orientation in pBAD24 relative to the expected wild-type transcription of the indicated genes in UTI89. [The A11* clone was orientated with respect to pBAD in the order ybiY (partial) - fsaA - moeB – moeA (partial)]

    Techniques Used: Clone Assay, Isolation

    Related Articles

    Purification:

    Article Title: High-throughput sequencing of sorted expression libraries reveals inhibitors of bacterial cell division
    Article Snippet: .. Purified genomic DNA from E. coli UTI89 was partially digested with FatI (New England Biolabs), a 4-bp-recognition endonuclease that includes the ATG start codon, which would increase the frequency of in-frame ORF ligation to the expression vector compared to other 4-bp-recognition endonucleases. ..

    Article Title: High-throughput sequencing of sorted expression libraries reveals inhibitors of bacterial cell division
    Article Snippet: .. The amplicons were purified and then partially digested with FatI (NEB). ..

    Article Title: High-throughput sequencing of sorted expression libraries reveals inhibitors of bacterial cell division
    Article Snippet: .. Construction of the shotgun UTI89 genomic DNA expression library Purified genomic DNA from E. coli UTI89 was partially digested with FatI (New England Biolabs), a 4-bp-recognition endonuclease that includes the ATG start codon, which would increase the frequency of in-frame ORF ligation to the expression vector compared to other 4-bp-recognition endonucleases. ..

    Ligation:

    Article Title: High-throughput sequencing of sorted expression libraries reveals inhibitors of bacterial cell division
    Article Snippet: .. Purified genomic DNA from E. coli UTI89 was partially digested with FatI (New England Biolabs), a 4-bp-recognition endonuclease that includes the ATG start codon, which would increase the frequency of in-frame ORF ligation to the expression vector compared to other 4-bp-recognition endonucleases. ..

    Article Title: High-throughput sequencing of sorted expression libraries reveals inhibitors of bacterial cell division
    Article Snippet: .. Construction of the shotgun UTI89 genomic DNA expression library Purified genomic DNA from E. coli UTI89 was partially digested with FatI (New England Biolabs), a 4-bp-recognition endonuclease that includes the ATG start codon, which would increase the frequency of in-frame ORF ligation to the expression vector compared to other 4-bp-recognition endonucleases. ..

    Expressing:

    Article Title: High-throughput sequencing of sorted expression libraries reveals inhibitors of bacterial cell division
    Article Snippet: .. Purified genomic DNA from E. coli UTI89 was partially digested with FatI (New England Biolabs), a 4-bp-recognition endonuclease that includes the ATG start codon, which would increase the frequency of in-frame ORF ligation to the expression vector compared to other 4-bp-recognition endonucleases. ..

    Article Title: High-throughput sequencing of sorted expression libraries reveals inhibitors of bacterial cell division
    Article Snippet: .. Construction of the shotgun UTI89 genomic DNA expression library Purified genomic DNA from E. coli UTI89 was partially digested with FatI (New England Biolabs), a 4-bp-recognition endonuclease that includes the ATG start codon, which would increase the frequency of in-frame ORF ligation to the expression vector compared to other 4-bp-recognition endonucleases. ..

    Plasmid Preparation:

    Article Title: High-throughput sequencing of sorted expression libraries reveals inhibitors of bacterial cell division
    Article Snippet: .. Purified genomic DNA from E. coli UTI89 was partially digested with FatI (New England Biolabs), a 4-bp-recognition endonuclease that includes the ATG start codon, which would increase the frequency of in-frame ORF ligation to the expression vector compared to other 4-bp-recognition endonucleases. ..

    Article Title: High-throughput sequencing of sorted expression libraries reveals inhibitors of bacterial cell division
    Article Snippet: .. Construction of the shotgun UTI89 genomic DNA expression library Purified genomic DNA from E. coli UTI89 was partially digested with FatI (New England Biolabs), a 4-bp-recognition endonuclease that includes the ATG start codon, which would increase the frequency of in-frame ORF ligation to the expression vector compared to other 4-bp-recognition endonucleases. ..

    Polymerase Chain Reaction:

    Article Title: ETV1 positively regulates transcription of tumor suppressor ARF
    Article Snippet: .. The PCR fragment was cut FatI (New England Biolabs, R0650) and RsaI (New England Biolabs, R0167) and inserted between EcoIRCI (Promega, R6951) and NcoI (New England Biolabs, R0193) sites of pGL3 Basic immediately upstream of the Luciferase gene. ..

    Luciferase:

    Article Title: ETV1 positively regulates transcription of tumor suppressor ARF
    Article Snippet: .. The PCR fragment was cut FatI (New England Biolabs, R0650) and RsaI (New England Biolabs, R0167) and inserted between EcoIRCI (Promega, R6951) and NcoI (New England Biolabs, R0193) sites of pGL3 Basic immediately upstream of the Luciferase gene. ..

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  • 91
    New England Biolabs fati
    Partial fragments of ORFs cause filamentation. <t>FatI</t> fragment sub-clones from the ybiY-fsaA-moeB-moeA and ybeM-tatE-lipA regions were screened (48 colonies each) for filamentation after growth and induction for 7 h at 37 °C. The indicated clones were identified as causing filamentation. a BW25113/pBAD24 control, ( b ) Clone H8 ( ybeM ), ( c ) Clone A2 ( ybeM ), ( d ) Clone H9 ( ybeM ), ( e ) Clone C2 ( ybeM ), ( f ) Clone D10 ( ybeM ), ( g ) Clone C1 ( ybiY ), ( h ) Clone A4 ( ybiY ), ( i ) Clone A11 ( ybiY-moeA ). j The ybeM-tatE-lipA region and sub-clones identified as capable of causing filamentation; three fragments throughout the 7 clones, of 112 bp, 87 bp and 29 bp were identified within the ybeM gene. k Map of fragments within the ybiY-fsaA-moeB-moeA locus capable of causing filamentation. Some of the fragments indicated in ( j ) and ( k ) were isolated in multiple clones (see Table 3).indicated in parentheses). The asterisk indicates that the expressed fragments in these clones were in the opposite orientation in pBAD24 relative to the expected wild-type transcription of the indicated genes in <t>UTI89.</t> [The A11* clone was orientated with respect to pBAD in the order ybiY (partial) - fsaA - moeB – moeA (partial)]
    Fati, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fati/product/New England Biolabs
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fati - by Bioz Stars, 2021-08
    91/100 stars
      Buy from Supplier

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    Partial fragments of ORFs cause filamentation. FatI fragment sub-clones from the ybiY-fsaA-moeB-moeA and ybeM-tatE-lipA regions were screened (48 colonies each) for filamentation after growth and induction for 7 h at 37 °C. The indicated clones were identified as causing filamentation. a BW25113/pBAD24 control, ( b ) Clone H8 ( ybeM ), ( c ) Clone A2 ( ybeM ), ( d ) Clone H9 ( ybeM ), ( e ) Clone C2 ( ybeM ), ( f ) Clone D10 ( ybeM ), ( g ) Clone C1 ( ybiY ), ( h ) Clone A4 ( ybiY ), ( i ) Clone A11 ( ybiY-moeA ). j The ybeM-tatE-lipA region and sub-clones identified as capable of causing filamentation; three fragments throughout the 7 clones, of 112 bp, 87 bp and 29 bp were identified within the ybeM gene. k Map of fragments within the ybiY-fsaA-moeB-moeA locus capable of causing filamentation. Some of the fragments indicated in ( j ) and ( k ) were isolated in multiple clones (see Table 3).indicated in parentheses). The asterisk indicates that the expressed fragments in these clones were in the opposite orientation in pBAD24 relative to the expected wild-type transcription of the indicated genes in UTI89. [The A11* clone was orientated with respect to pBAD in the order ybiY (partial) - fsaA - moeB – moeA (partial)]

    Journal: BMC Genomics

    Article Title: High-throughput sequencing of sorted expression libraries reveals inhibitors of bacterial cell division

    doi: 10.1186/s12864-018-5187-7

    Figure Lengend Snippet: Partial fragments of ORFs cause filamentation. FatI fragment sub-clones from the ybiY-fsaA-moeB-moeA and ybeM-tatE-lipA regions were screened (48 colonies each) for filamentation after growth and induction for 7 h at 37 °C. The indicated clones were identified as causing filamentation. a BW25113/pBAD24 control, ( b ) Clone H8 ( ybeM ), ( c ) Clone A2 ( ybeM ), ( d ) Clone H9 ( ybeM ), ( e ) Clone C2 ( ybeM ), ( f ) Clone D10 ( ybeM ), ( g ) Clone C1 ( ybiY ), ( h ) Clone A4 ( ybiY ), ( i ) Clone A11 ( ybiY-moeA ). j The ybeM-tatE-lipA region and sub-clones identified as capable of causing filamentation; three fragments throughout the 7 clones, of 112 bp, 87 bp and 29 bp were identified within the ybeM gene. k Map of fragments within the ybiY-fsaA-moeB-moeA locus capable of causing filamentation. Some of the fragments indicated in ( j ) and ( k ) were isolated in multiple clones (see Table 3).indicated in parentheses). The asterisk indicates that the expressed fragments in these clones were in the opposite orientation in pBAD24 relative to the expected wild-type transcription of the indicated genes in UTI89. [The A11* clone was orientated with respect to pBAD in the order ybiY (partial) - fsaA - moeB – moeA (partial)]

    Article Snippet: Purified genomic DNA from E. coli UTI89 was partially digested with FatI (New England Biolabs), a 4-bp-recognition endonuclease that includes the ATG start codon, which would increase the frequency of in-frame ORF ligation to the expression vector compared to other 4-bp-recognition endonucleases.

    Techniques: Clone Assay, Isolation

    Partial fragments of ORFs cause filamentation. FatI fragment sub-clones from the ybiY-fsaA-moeB-moeA and ybeM-tatE-lipA regions were screened (48 colonies each) for filamentation after growth and induction for 7 h at 37 °C. The indicated clones were identified as causing filamentation. a BW25113/pBAD24 control, ( b ) Clone H8 ( ybeM ), ( c ) Clone A2 ( ybeM ), ( d ) Clone H9 ( ybeM ), ( e ) Clone C2 ( ybeM ), ( f ) Clone D10 ( ybeM ), ( g ) Clone C1 ( ybiY ), ( h ) Clone A4 ( ybiY ), ( i ) Clone A11 ( ybiY-moeA ). j The ybeM-tatE-lipA region and sub-clones identified as capable of causing filamentation; three fragments throughout the 7 clones, of 112 bp, 87 bp and 29 bp were identified within the ybeM gene. k Map of fragments within the ybiY-fsaA-moeB-moeA locus capable of causing filamentation. Some of the fragments indicated in ( j ) and ( k ) were isolated in multiple clones (see Table 3).indicated in parentheses). The asterisk indicates that the expressed fragments in these clones were in the opposite orientation in pBAD24 relative to the expected wild-type transcription of the indicated genes in UTI89. [The A11* clone was orientated with respect to pBAD in the order ybiY (partial) - fsaA - moeB – moeA (partial)]

    Journal: BMC Genomics

    Article Title: High-throughput sequencing of sorted expression libraries reveals inhibitors of bacterial cell division

    doi: 10.1186/s12864-018-5187-7

    Figure Lengend Snippet: Partial fragments of ORFs cause filamentation. FatI fragment sub-clones from the ybiY-fsaA-moeB-moeA and ybeM-tatE-lipA regions were screened (48 colonies each) for filamentation after growth and induction for 7 h at 37 °C. The indicated clones were identified as causing filamentation. a BW25113/pBAD24 control, ( b ) Clone H8 ( ybeM ), ( c ) Clone A2 ( ybeM ), ( d ) Clone H9 ( ybeM ), ( e ) Clone C2 ( ybeM ), ( f ) Clone D10 ( ybeM ), ( g ) Clone C1 ( ybiY ), ( h ) Clone A4 ( ybiY ), ( i ) Clone A11 ( ybiY-moeA ). j The ybeM-tatE-lipA region and sub-clones identified as capable of causing filamentation; three fragments throughout the 7 clones, of 112 bp, 87 bp and 29 bp were identified within the ybeM gene. k Map of fragments within the ybiY-fsaA-moeB-moeA locus capable of causing filamentation. Some of the fragments indicated in ( j ) and ( k ) were isolated in multiple clones (see Table 3).indicated in parentheses). The asterisk indicates that the expressed fragments in these clones were in the opposite orientation in pBAD24 relative to the expected wild-type transcription of the indicated genes in UTI89. [The A11* clone was orientated with respect to pBAD in the order ybiY (partial) - fsaA - moeB – moeA (partial)]

    Article Snippet: Construction of the shotgun UTI89 genomic DNA expression library Purified genomic DNA from E. coli UTI89 was partially digested with FatI (New England Biolabs), a 4-bp-recognition endonuclease that includes the ATG start codon, which would increase the frequency of in-frame ORF ligation to the expression vector compared to other 4-bp-recognition endonucleases.

    Techniques: Clone Assay, Isolation