bpu 10i  (New England Biolabs)


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    Name:
    Bpu10I
    Description:
    Bpu10I 1 000 units
    Catalog Number:
    r0649l
    Price:
    282
    Size:
    1 000 units
    Category:
    Restriction Enzymes
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    New England Biolabs bpu 10i
    Bpu10I
    Bpu10I 1 000 units
    https://www.bioz.com/result/bpu 10i/product/New England Biolabs
    Average 93 stars, based on 262 article reviews
    Price from $9.99 to $1999.99
    bpu 10i - by Bioz Stars, 2020-08
    93/100 stars

    Images

    1) Product Images from "Association of the programmed cell death 1 (PDCD1) gene polymorphism with ankylosing spondylitis in the Korean population"

    Article Title: Association of the programmed cell death 1 (PDCD1) gene polymorphism with ankylosing spondylitis in the Korean population

    Journal: Arthritis Research & Therapy

    doi: 10.1186/ar2071

    Gel electrophoresis patterns of PD-1.5 and PD-1.9. (a) The amplified fragments of PD-1.5 were digested with Alu I: the polymerase chain reaction (PCR) product size was 333 base pairs (bp), which was digested to 264 and 69 bp. If the product was digested, the allele was identified as T; if not, it was identified as C. (b) The amplified fragments of PD-1.9 were digested with Bpu 10I: the PCR product size was 408 bp, which was digested to 260 and 145 bp. If the product was digested, the allele was identified as C; if not, it was identified as T.
    Figure Legend Snippet: Gel electrophoresis patterns of PD-1.5 and PD-1.9. (a) The amplified fragments of PD-1.5 were digested with Alu I: the polymerase chain reaction (PCR) product size was 333 base pairs (bp), which was digested to 264 and 69 bp. If the product was digested, the allele was identified as T; if not, it was identified as C. (b) The amplified fragments of PD-1.9 were digested with Bpu 10I: the PCR product size was 408 bp, which was digested to 260 and 145 bp. If the product was digested, the allele was identified as C; if not, it was identified as T.

    Techniques Used: Nucleic Acid Electrophoresis, Amplification, Polymerase Chain Reaction

    2) Product Images from "Retinal pathology and skin barrier defect in mice carrying a Stargardt disease-3 mutation in elongase of very long chain fatty acids-4"

    Article Title: Retinal pathology and skin barrier defect in mice carrying a Stargardt disease-3 mutation in elongase of very long chain fatty acids-4

    Journal: Molecular Vision

    doi:

    Southern blot verification of Stgd3 mouse genotype. A : Genomic DNA was digested with Nsi I and probed with a 5'-probe located upstream of the recombinant targeted site (see Figure 1 ). A signal from the wt allele as well as an extra band (Rec), derived from the recombinant allele, were detected in the Neo/wt DNA digests. The size of the Rec band was consistent with the 11.8-kb size predicted for the homologous recombination event, confirming the presence of recombination in these mice. In the Stgd3/wt DNA digests, the size of the recombined band was reduced due to loss of the Neo cassette with the result that the wt and Stgd3 allele-derived signals were not resolved. B : Hybridization with the Neo probe detected the presence of the Neo signal in the Neo/wt but not in Stgd3/wt DNA digests, confirming recombination of the targeting sequence and Cre-mediated excision of the Neo cassette, respectively. The Neo probe contained a mouse phosphoglycerate kinase ( PGK ) promoter and thus also detected the endogenous PGK -gene sequence in all mouse DNA samples. C : Introduction of the STGD3 mutation into Elovl4 exon 6 generated a new Bpu 10I restriction site in this exon. The presence of the STGD3-mutation in the DNA of Neo/wt and Stgd3/wt mice was confirmed by the detection of a new 4.3-kb Bpu 10I restriction fragment that was absent from wt/wt DNA.
    Figure Legend Snippet: Southern blot verification of Stgd3 mouse genotype. A : Genomic DNA was digested with Nsi I and probed with a 5'-probe located upstream of the recombinant targeted site (see Figure 1 ). A signal from the wt allele as well as an extra band (Rec), derived from the recombinant allele, were detected in the Neo/wt DNA digests. The size of the Rec band was consistent with the 11.8-kb size predicted for the homologous recombination event, confirming the presence of recombination in these mice. In the Stgd3/wt DNA digests, the size of the recombined band was reduced due to loss of the Neo cassette with the result that the wt and Stgd3 allele-derived signals were not resolved. B : Hybridization with the Neo probe detected the presence of the Neo signal in the Neo/wt but not in Stgd3/wt DNA digests, confirming recombination of the targeting sequence and Cre-mediated excision of the Neo cassette, respectively. The Neo probe contained a mouse phosphoglycerate kinase ( PGK ) promoter and thus also detected the endogenous PGK -gene sequence in all mouse DNA samples. C : Introduction of the STGD3 mutation into Elovl4 exon 6 generated a new Bpu 10I restriction site in this exon. The presence of the STGD3-mutation in the DNA of Neo/wt and Stgd3/wt mice was confirmed by the detection of a new 4.3-kb Bpu 10I restriction fragment that was absent from wt/wt DNA.

    Techniques Used: Southern Blot, Recombinant, Derivative Assay, Homologous Recombination, Mouse Assay, Hybridization, Sequencing, Mutagenesis, Generated

    Gene-targeting strategy for generation of Stgd3 -gene knockin mice. A : Partial nucleotide sequence from exon 6, and the encoded amino acids (capital letters), of the wt ( Elovl4 ) and mutant ( Stgd3 ) alleles. Shown are the mutations introduced into the Elovl4 sequence prior to assembly of the targeting construct. Five nucleotide base pairs, corresponding to those absent in Stargardt-3 patients, were deleted (del). Two point mutations (the altered and substituted nucleotides are shown in blue) were introduced in the sequence downstream of the deletion to generate a new C-terminal sequence that is identical to that found in STGD3 patients. B : Schematic maps of a portion of the Elovl4 allele encompassing exons 4, 5, and 6, the targeting construct, recombinant allele and the final Stgd3 allele. Restriction sites used for cloning and for Southern blot verification of recombination and deletion events, as well as the location of the Southern probes are included. Mutations in exon 6 are shown schematically as a shaded box, within which the new Bpu 10I restriction site introduced during sequence manipulation is shown. Homologous recombination of the 11.5-kb targeting construct generates a recombinant allele containing exon 6 with the Stgd3 mutation, a neomycin selection cassette ( Neo ) flanked by lox P sites (L), and 4.2 kb and 3.1kb of 5'- and 3'-targeting sequence, respectively. Breeding of recombinant Neo/wt mice with Cre -transgenic mice leads to Cre-mediated deletion of the Neo cassette and generation of the mice carrying the final Stgd3 allele.
    Figure Legend Snippet: Gene-targeting strategy for generation of Stgd3 -gene knockin mice. A : Partial nucleotide sequence from exon 6, and the encoded amino acids (capital letters), of the wt ( Elovl4 ) and mutant ( Stgd3 ) alleles. Shown are the mutations introduced into the Elovl4 sequence prior to assembly of the targeting construct. Five nucleotide base pairs, corresponding to those absent in Stargardt-3 patients, were deleted (del). Two point mutations (the altered and substituted nucleotides are shown in blue) were introduced in the sequence downstream of the deletion to generate a new C-terminal sequence that is identical to that found in STGD3 patients. B : Schematic maps of a portion of the Elovl4 allele encompassing exons 4, 5, and 6, the targeting construct, recombinant allele and the final Stgd3 allele. Restriction sites used for cloning and for Southern blot verification of recombination and deletion events, as well as the location of the Southern probes are included. Mutations in exon 6 are shown schematically as a shaded box, within which the new Bpu 10I restriction site introduced during sequence manipulation is shown. Homologous recombination of the 11.5-kb targeting construct generates a recombinant allele containing exon 6 with the Stgd3 mutation, a neomycin selection cassette ( Neo ) flanked by lox P sites (L), and 4.2 kb and 3.1kb of 5'- and 3'-targeting sequence, respectively. Breeding of recombinant Neo/wt mice with Cre -transgenic mice leads to Cre-mediated deletion of the Neo cassette and generation of the mice carrying the final Stgd3 allele.

    Techniques Used: Knock-In, Mouse Assay, Sequencing, Mutagenesis, Construct, Recombinant, Clone Assay, Southern Blot, Homologous Recombination, Selection, Transgenic Assay

    Related Articles

    Agarose Gel Electrophoresis:

    Article Title: Retinal pathology and skin barrier defect in mice carrying a Stargardt disease-3 mutation in elongase of very long chain fatty acids-4
    Article Snippet: .. Southern blot analysis of knockin mice Tail DNA samples were digested with Nsi I or Bpu 10I (New England Biolabs, Beverly, MA), separated on an agarose gel, transferred to Hybond N+ membrane (GE Healthcare, Piscataway, NJ), and subjected to Southern blot analysis using P32-labeled DNA probes: a 453-bp 5'-external probe, a 284-bp Neo probe, or a 1.2-kb 3'-internal probe ( ). .. Polymerase chain reaction genotyping of mice After founder mice were characterized by Southern blot analysis, further genotyping of their progenies was performed by PCR using tail DNA.

    Southern Blot:

    Article Title: Retinal pathology and skin barrier defect in mice carrying a Stargardt disease-3 mutation in elongase of very long chain fatty acids-4
    Article Snippet: .. Southern blot analysis of knockin mice Tail DNA samples were digested with Nsi I or Bpu 10I (New England Biolabs, Beverly, MA), separated on an agarose gel, transferred to Hybond N+ membrane (GE Healthcare, Piscataway, NJ), and subjected to Southern blot analysis using P32-labeled DNA probes: a 453-bp 5'-external probe, a 284-bp Neo probe, or a 1.2-kb 3'-internal probe ( ). .. Polymerase chain reaction genotyping of mice After founder mice were characterized by Southern blot analysis, further genotyping of their progenies was performed by PCR using tail DNA.

    Construct:

    Article Title: Exogenous Transposable Elements Circumvent Identity-Based Silencing, Permitting the Dissection of Expression-Dependent Silencing [OPEN]
    Article Snippet: .. The solo Tto LTR construct was linearized with Bpu10I and treated with Shrimp Alkaline Phosphatase (NEB). .. These two products were then ligated together using T4 DNA ligase, and colonies were screened for the appropriate direction of ligation.

    Article Title: Post-Transcriptional Regulation of Toll-Interacting Protein in the Intestinal Epithelium
    Article Snippet: .. Luc-mTollip_3´-UTR Δ550–1876 was constructed by self-ligation of the blunt-ended product treated with Bpu10I (New England Biolabs, Ipswich, MA). .. For the construction of Luc-mTollip_3´-UTR Δ1–2572 and Δ1–2646, each deletion fragment of the 3´-UTR of the mouse Tollip gene was obtained by PCR and inserted into the BamHI and XbaI sites.

    Mouse Assay:

    Article Title: Retinal pathology and skin barrier defect in mice carrying a Stargardt disease-3 mutation in elongase of very long chain fatty acids-4
    Article Snippet: .. Southern blot analysis of knockin mice Tail DNA samples were digested with Nsi I or Bpu 10I (New England Biolabs, Beverly, MA), separated on an agarose gel, transferred to Hybond N+ membrane (GE Healthcare, Piscataway, NJ), and subjected to Southern blot analysis using P32-labeled DNA probes: a 453-bp 5'-external probe, a 284-bp Neo probe, or a 1.2-kb 3'-internal probe ( ). .. Polymerase chain reaction genotyping of mice After founder mice were characterized by Southern blot analysis, further genotyping of their progenies was performed by PCR using tail DNA.

    Knock-In:

    Article Title: Retinal pathology and skin barrier defect in mice carrying a Stargardt disease-3 mutation in elongase of very long chain fatty acids-4
    Article Snippet: .. Southern blot analysis of knockin mice Tail DNA samples were digested with Nsi I or Bpu 10I (New England Biolabs, Beverly, MA), separated on an agarose gel, transferred to Hybond N+ membrane (GE Healthcare, Piscataway, NJ), and subjected to Southern blot analysis using P32-labeled DNA probes: a 453-bp 5'-external probe, a 284-bp Neo probe, or a 1.2-kb 3'-internal probe ( ). .. Polymerase chain reaction genotyping of mice After founder mice were characterized by Southern blot analysis, further genotyping of their progenies was performed by PCR using tail DNA.

    Plasmid Preparation:

    Article Title: Directed evolution of broadly crossreactive chemokine-blocking antibodies efficacious in arthritis
    Article Snippet: .. Genes encoding N CXCL-(G3 )-c-myc-Aga2pC fusion proteins were further sub-cloned into a new pCT vector via Bpu 10I and Xho I (New England BioLabs) restriction enzymes except for mCXCL2 for which Pst I-HF and Xho I (New England BioLabs) restriction enzymes were used. .. All constructs were verified by DNA sequencing and termed N CXCL-Aga2pC fusion proteins (see Supplementary Data for information about protein accession numbers, oligonucleotide primers, DNA, and amino-acid sequences).

    Article Title: Directed evolution of broadly crossreactive chemokine-blocking antibodies efficacious in arthritis
    Article Snippet: .. Gene encoding N hCXCL1WT -(G3 )-c-myc-Aga2pC fusion protein was sub-cloned into a new pCT vector via Bpu 10I and Xho I (New England BioLabs) restriction enzymes. .. The obtained pCT-hCXCL1WT -Aga2 vector was used as the template for the site-directed mutagenesis.

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  • 93
    New England Biolabs bpu 10i
    Gel electrophoresis patterns of PD-1.5 and PD-1.9. (a) The amplified fragments of PD-1.5 were digested with Alu I: the polymerase chain reaction (PCR) product size was 333 base pairs (bp), which was digested to 264 and 69 bp. If the product was digested, the allele was identified as T; if not, it was identified as C. (b) The amplified fragments of PD-1.9 were digested with <t>Bpu</t> <t>10I:</t> the PCR product size was 408 bp, which was digested to 260 and 145 bp. If the product was digested, the allele was identified as C; if not, it was identified as T.
    Bpu 10i, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bpu 10i/product/New England Biolabs
    Average 93 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    bpu 10i - by Bioz Stars, 2020-08
    93/100 stars
      Buy from Supplier

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    Gel electrophoresis patterns of PD-1.5 and PD-1.9. (a) The amplified fragments of PD-1.5 were digested with Alu I: the polymerase chain reaction (PCR) product size was 333 base pairs (bp), which was digested to 264 and 69 bp. If the product was digested, the allele was identified as T; if not, it was identified as C. (b) The amplified fragments of PD-1.9 were digested with Bpu 10I: the PCR product size was 408 bp, which was digested to 260 and 145 bp. If the product was digested, the allele was identified as C; if not, it was identified as T.

    Journal: Arthritis Research & Therapy

    Article Title: Association of the programmed cell death 1 (PDCD1) gene polymorphism with ankylosing spondylitis in the Korean population

    doi: 10.1186/ar2071

    Figure Lengend Snippet: Gel electrophoresis patterns of PD-1.5 and PD-1.9. (a) The amplified fragments of PD-1.5 were digested with Alu I: the polymerase chain reaction (PCR) product size was 333 base pairs (bp), which was digested to 264 and 69 bp. If the product was digested, the allele was identified as T; if not, it was identified as C. (b) The amplified fragments of PD-1.9 were digested with Bpu 10I: the PCR product size was 408 bp, which was digested to 260 and 145 bp. If the product was digested, the allele was identified as C; if not, it was identified as T.

    Article Snippet: The subsequent restriction-fragment-length polymorphism (RFLP) analysis was performed to determine the PD-1.5 C/T (dbSNP rs#cluster id. rs2227981) and PD-1.9 C/T (dbSNP rs#cluster id. rs2227982) SNPs using Alu I (New England Biolabs Inc., Beverly, MA, USA) and Bpu 10I (New England Biolabs Inc), respectively (Table ) [ ].

    Techniques: Nucleic Acid Electrophoresis, Amplification, Polymerase Chain Reaction

    Southern blot verification of Stgd3 mouse genotype. A : Genomic DNA was digested with Nsi I and probed with a 5'-probe located upstream of the recombinant targeted site (see Figure 1 ). A signal from the wt allele as well as an extra band (Rec), derived from the recombinant allele, were detected in the Neo/wt DNA digests. The size of the Rec band was consistent with the 11.8-kb size predicted for the homologous recombination event, confirming the presence of recombination in these mice. In the Stgd3/wt DNA digests, the size of the recombined band was reduced due to loss of the Neo cassette with the result that the wt and Stgd3 allele-derived signals were not resolved. B : Hybridization with the Neo probe detected the presence of the Neo signal in the Neo/wt but not in Stgd3/wt DNA digests, confirming recombination of the targeting sequence and Cre-mediated excision of the Neo cassette, respectively. The Neo probe contained a mouse phosphoglycerate kinase ( PGK ) promoter and thus also detected the endogenous PGK -gene sequence in all mouse DNA samples. C : Introduction of the STGD3 mutation into Elovl4 exon 6 generated a new Bpu 10I restriction site in this exon. The presence of the STGD3-mutation in the DNA of Neo/wt and Stgd3/wt mice was confirmed by the detection of a new 4.3-kb Bpu 10I restriction fragment that was absent from wt/wt DNA.

    Journal: Molecular Vision

    Article Title: Retinal pathology and skin barrier defect in mice carrying a Stargardt disease-3 mutation in elongase of very long chain fatty acids-4

    doi:

    Figure Lengend Snippet: Southern blot verification of Stgd3 mouse genotype. A : Genomic DNA was digested with Nsi I and probed with a 5'-probe located upstream of the recombinant targeted site (see Figure 1 ). A signal from the wt allele as well as an extra band (Rec), derived from the recombinant allele, were detected in the Neo/wt DNA digests. The size of the Rec band was consistent with the 11.8-kb size predicted for the homologous recombination event, confirming the presence of recombination in these mice. In the Stgd3/wt DNA digests, the size of the recombined band was reduced due to loss of the Neo cassette with the result that the wt and Stgd3 allele-derived signals were not resolved. B : Hybridization with the Neo probe detected the presence of the Neo signal in the Neo/wt but not in Stgd3/wt DNA digests, confirming recombination of the targeting sequence and Cre-mediated excision of the Neo cassette, respectively. The Neo probe contained a mouse phosphoglycerate kinase ( PGK ) promoter and thus also detected the endogenous PGK -gene sequence in all mouse DNA samples. C : Introduction of the STGD3 mutation into Elovl4 exon 6 generated a new Bpu 10I restriction site in this exon. The presence of the STGD3-mutation in the DNA of Neo/wt and Stgd3/wt mice was confirmed by the detection of a new 4.3-kb Bpu 10I restriction fragment that was absent from wt/wt DNA.

    Article Snippet: Southern blot analysis of knockin mice Tail DNA samples were digested with Nsi I or Bpu 10I (New England Biolabs, Beverly, MA), separated on an agarose gel, transferred to Hybond N+ membrane (GE Healthcare, Piscataway, NJ), and subjected to Southern blot analysis using P32-labeled DNA probes: a 453-bp 5'-external probe, a 284-bp Neo probe, or a 1.2-kb 3'-internal probe ( ).

    Techniques: Southern Blot, Recombinant, Derivative Assay, Homologous Recombination, Mouse Assay, Hybridization, Sequencing, Mutagenesis, Generated

    Gene-targeting strategy for generation of Stgd3 -gene knockin mice. A : Partial nucleotide sequence from exon 6, and the encoded amino acids (capital letters), of the wt ( Elovl4 ) and mutant ( Stgd3 ) alleles. Shown are the mutations introduced into the Elovl4 sequence prior to assembly of the targeting construct. Five nucleotide base pairs, corresponding to those absent in Stargardt-3 patients, were deleted (del). Two point mutations (the altered and substituted nucleotides are shown in blue) were introduced in the sequence downstream of the deletion to generate a new C-terminal sequence that is identical to that found in STGD3 patients. B : Schematic maps of a portion of the Elovl4 allele encompassing exons 4, 5, and 6, the targeting construct, recombinant allele and the final Stgd3 allele. Restriction sites used for cloning and for Southern blot verification of recombination and deletion events, as well as the location of the Southern probes are included. Mutations in exon 6 are shown schematically as a shaded box, within which the new Bpu 10I restriction site introduced during sequence manipulation is shown. Homologous recombination of the 11.5-kb targeting construct generates a recombinant allele containing exon 6 with the Stgd3 mutation, a neomycin selection cassette ( Neo ) flanked by lox P sites (L), and 4.2 kb and 3.1kb of 5'- and 3'-targeting sequence, respectively. Breeding of recombinant Neo/wt mice with Cre -transgenic mice leads to Cre-mediated deletion of the Neo cassette and generation of the mice carrying the final Stgd3 allele.

    Journal: Molecular Vision

    Article Title: Retinal pathology and skin barrier defect in mice carrying a Stargardt disease-3 mutation in elongase of very long chain fatty acids-4

    doi:

    Figure Lengend Snippet: Gene-targeting strategy for generation of Stgd3 -gene knockin mice. A : Partial nucleotide sequence from exon 6, and the encoded amino acids (capital letters), of the wt ( Elovl4 ) and mutant ( Stgd3 ) alleles. Shown are the mutations introduced into the Elovl4 sequence prior to assembly of the targeting construct. Five nucleotide base pairs, corresponding to those absent in Stargardt-3 patients, were deleted (del). Two point mutations (the altered and substituted nucleotides are shown in blue) were introduced in the sequence downstream of the deletion to generate a new C-terminal sequence that is identical to that found in STGD3 patients. B : Schematic maps of a portion of the Elovl4 allele encompassing exons 4, 5, and 6, the targeting construct, recombinant allele and the final Stgd3 allele. Restriction sites used for cloning and for Southern blot verification of recombination and deletion events, as well as the location of the Southern probes are included. Mutations in exon 6 are shown schematically as a shaded box, within which the new Bpu 10I restriction site introduced during sequence manipulation is shown. Homologous recombination of the 11.5-kb targeting construct generates a recombinant allele containing exon 6 with the Stgd3 mutation, a neomycin selection cassette ( Neo ) flanked by lox P sites (L), and 4.2 kb and 3.1kb of 5'- and 3'-targeting sequence, respectively. Breeding of recombinant Neo/wt mice with Cre -transgenic mice leads to Cre-mediated deletion of the Neo cassette and generation of the mice carrying the final Stgd3 allele.

    Article Snippet: Southern blot analysis of knockin mice Tail DNA samples were digested with Nsi I or Bpu 10I (New England Biolabs, Beverly, MA), separated on an agarose gel, transferred to Hybond N+ membrane (GE Healthcare, Piscataway, NJ), and subjected to Southern blot analysis using P32-labeled DNA probes: a 453-bp 5'-external probe, a 284-bp Neo probe, or a 1.2-kb 3'-internal probe ( ).

    Techniques: Knock-In, Mouse Assay, Sequencing, Mutagenesis, Construct, Recombinant, Clone Assay, Southern Blot, Homologous Recombination, Selection, Transgenic Assay