btsc i  (New England Biolabs)


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    Name:
    BtsCI
    Description:
    BtsCI 2 000 units
    Catalog Number:
    r0647l
    Price:
    70
    Size:
    2 000 units
    Category:
    Restriction Enzymes
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    Structured Review

    New England Biolabs btsc i
    BtsCI
    BtsCI 2 000 units
    https://www.bioz.com/result/btsc i/product/New England Biolabs
    Average 95 stars, based on 29 article reviews
    Price from $9.99 to $1999.99
    btsc i - by Bioz Stars, 2020-08
    95/100 stars

    Images

    1) Product Images from "Skin Characteristics in Patients with Pityriasis Versicolor Using Non-Invasive Method, MPA5"

    Article Title: Skin Characteristics in Patients with Pityriasis Versicolor Using Non-Invasive Method, MPA5

    Journal: Annals of Dermatology

    doi: 10.5021/ad.2012.24.4.444

    PCR-RFLP patterns of 26S rDNA PCR digested with restriction enzymes (A) Hha I, (B) BtsC I of 11 Malassezia standard strains in hyperpigmented lesions of 21 patients. Lanes: M: molecular Marker, 1: Malassezia globosa (CBS7966), 2: M. globosa (CBS7966), 3: M. restricta (KCTC7848), 4: M. globosa (CBS7966), 5: M. restricta (KCTC7848), 6: M. restricta (KCTC7848), 7: M. restricta (KCTC7848), 8: M. slooffiae (KCTC17431), 9: M. globosa (CBS7966), 10: M. globosa (CBS7966), 11: M. restricta (KCTC7848), 12: M. globosa (CBS7966), 13: M. globosa (CBS7966), 14: M. furfur (KCTC7743), 15: M. slooffiae (KCTC17431), 16: M. globosa (CBS7966), 17: M. globosa (CBS7966), 18: M. restricta (KCTC7848), 19: M. restricta (KCTC7848), 20: M. furfur (KCTC7743), 21: M. sympodialis (KCTC7985). PCR: polymerase chain reaction, RFLP: restriction fragment length polymorphism.
    Figure Legend Snippet: PCR-RFLP patterns of 26S rDNA PCR digested with restriction enzymes (A) Hha I, (B) BtsC I of 11 Malassezia standard strains in hyperpigmented lesions of 21 patients. Lanes: M: molecular Marker, 1: Malassezia globosa (CBS7966), 2: M. globosa (CBS7966), 3: M. restricta (KCTC7848), 4: M. globosa (CBS7966), 5: M. restricta (KCTC7848), 6: M. restricta (KCTC7848), 7: M. restricta (KCTC7848), 8: M. slooffiae (KCTC17431), 9: M. globosa (CBS7966), 10: M. globosa (CBS7966), 11: M. restricta (KCTC7848), 12: M. globosa (CBS7966), 13: M. globosa (CBS7966), 14: M. furfur (KCTC7743), 15: M. slooffiae (KCTC17431), 16: M. globosa (CBS7966), 17: M. globosa (CBS7966), 18: M. restricta (KCTC7848), 19: M. restricta (KCTC7848), 20: M. furfur (KCTC7743), 21: M. sympodialis (KCTC7985). PCR: polymerase chain reaction, RFLP: restriction fragment length polymorphism.

    Techniques Used: Polymerase Chain Reaction, Marker

    PCR-RFLP patterns of 26S rDNA PCR digested with restriction enzymes (A) Hha I, (B) BtsC I of 11 Malassezia standard strains in hypopigmented lesions of 9 patients. Lanes: M: molecular Marker , 1: Malassezia slooffiae (KCTC17431), 2: M. globosa (CBS7966), 3: M. restricta (KCTC7848), 4: M. restricta (KCTC7848), 5: M. globosa (CBS7966), 6: M. globosa (CBS7966), 7: M. globosa (CBS7966), 8: M. sympodialis (KCTC7985), 9: M. furfur (KCTC7743). PCR: polymerase chain reaction, RFLP: restriction fragment length polymorphism.
    Figure Legend Snippet: PCR-RFLP patterns of 26S rDNA PCR digested with restriction enzymes (A) Hha I, (B) BtsC I of 11 Malassezia standard strains in hypopigmented lesions of 9 patients. Lanes: M: molecular Marker , 1: Malassezia slooffiae (KCTC17431), 2: M. globosa (CBS7966), 3: M. restricta (KCTC7848), 4: M. restricta (KCTC7848), 5: M. globosa (CBS7966), 6: M. globosa (CBS7966), 7: M. globosa (CBS7966), 8: M. sympodialis (KCTC7985), 9: M. furfur (KCTC7743). PCR: polymerase chain reaction, RFLP: restriction fragment length polymorphism.

    Techniques Used: Polymerase Chain Reaction, Marker

    2) Product Images from "Mice expressing a human KATP channel mutation have altered channel ATP sensitivity but no cardiac abnormalities"

    Article Title: Mice expressing a human KATP channel mutation have altered channel ATP sensitivity but no cardiac abnormalities

    Journal: Diabetologia

    doi: 10.1007/s00125-011-2428-6

    Kcnj11 expression in the heart. a Kcnj11 expression in tissue isolated from ROSA, WT, Mck-Cre and m-V59M mice. WT, but not mutant (V59M), Kcnj11 cDNA is cut by the restriction enzyme BtsCI: the presence of two bands thus indicates the presence of the WT gene only and three bands indicates both WT and mutant genes. Data are representative of experiments on seven ROSA, four m-V59M, four WT and four Mck-Cre mice. sk1, quadriceps muscle; sk2, triceps muscle; sk3, diaphragm. b,c Quantitative PCR showing expression of Kcnj11 in heart and brain ( b ) and Gfp in heart ( c ) of control (grey bars, n = 5) and m-V59M mice (white bars, n = 5), relative to a panel of housekeeping genes. * p
    Figure Legend Snippet: Kcnj11 expression in the heart. a Kcnj11 expression in tissue isolated from ROSA, WT, Mck-Cre and m-V59M mice. WT, but not mutant (V59M), Kcnj11 cDNA is cut by the restriction enzyme BtsCI: the presence of two bands thus indicates the presence of the WT gene only and three bands indicates both WT and mutant genes. Data are representative of experiments on seven ROSA, four m-V59M, four WT and four Mck-Cre mice. sk1, quadriceps muscle; sk2, triceps muscle; sk3, diaphragm. b,c Quantitative PCR showing expression of Kcnj11 in heart and brain ( b ) and Gfp in heart ( c ) of control (grey bars, n = 5) and m-V59M mice (white bars, n = 5), relative to a panel of housekeeping genes. * p

    Techniques Used: Expressing, Isolation, Mouse Assay, Mutagenesis, Real-time Polymerase Chain Reaction

    3) Product Images from "Reduction of Soybean Meal Non-Starch Polysaccharides and ?-Galactosides by Solid-State Fermentation Using Cellulolytic Bacteria Obtained from Different Environments"

    Article Title: Reduction of Soybean Meal Non-Starch Polysaccharides and ?-Galactosides by Solid-State Fermentation Using Cellulolytic Bacteria Obtained from Different Environments

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0044783

    Dominance of the inoculated strains after SSF as revealed by their RFLP profiles. The RFLP profiles from the 16S rRNA genes of the selected strains (S7, CR18 and T5) were generated using a culture-independent approach. DNA was extracted directly from FSBM (inoculated and non-Inoculated groups), and the PCR amplicons were digested with the restriction enzymes Bmt I and BtsC I. The figure shows the RFLPs of the selected strains (S7, CR18 and T5) and the RFLP profiles from five replicates of inoculated and non-inoculated SSFs. The arrows indicate the presence of the S7 and T5 strains as dominant in the fermentation process.
    Figure Legend Snippet: Dominance of the inoculated strains after SSF as revealed by their RFLP profiles. The RFLP profiles from the 16S rRNA genes of the selected strains (S7, CR18 and T5) were generated using a culture-independent approach. DNA was extracted directly from FSBM (inoculated and non-Inoculated groups), and the PCR amplicons were digested with the restriction enzymes Bmt I and BtsC I. The figure shows the RFLPs of the selected strains (S7, CR18 and T5) and the RFLP profiles from five replicates of inoculated and non-inoculated SSFs. The arrows indicate the presence of the S7 and T5 strains as dominant in the fermentation process.

    Techniques Used: Generated, Polymerase Chain Reaction

    Related Articles

    Amplification:

    Article Title: Lack of effect of apolipoprotein C3 polymorphisms on indices of liver steatosis, lipid profile and insulin resistance in obese Southern Europeans
    Article Snippet: .. A 497-bp fragment was amplified and enzymatically restricted using BtsCI (New England Biolabs, Ipswich, MA, USA). .. The resulted enzyme-digested fragments of the PCR products were fractionated on 3.5% agarose gels, stained with ethidium bromide, and visualized with an imaging system (Amersham-Pharmacia Biosciences).

    Agarose Gel Electrophoresis:

    Article Title: Restoration of Vision in the pde6?-deficient Dog, a Large Animal Model of Rod-cone Dystrophy
    Article Snippet: .. Pde6β PCR products were purified by Nucleospin Extract II (Macherey-Nagel, Hoerdt, France) before digestion by BtsCI (New England Biolabs, Ipswich, MA) for 2 hours at 50 °C according to the manufacturer's instructions and analyzed by 3.5% agarose gel electrophoresis. ..

    Purification:

    Article Title: Restoration of Vision in the pde6?-deficient Dog, a Large Animal Model of Rod-cone Dystrophy
    Article Snippet: .. Pde6β PCR products were purified by Nucleospin Extract II (Macherey-Nagel, Hoerdt, France) before digestion by BtsCI (New England Biolabs, Ipswich, MA) for 2 hours at 50 °C according to the manufacturer's instructions and analyzed by 3.5% agarose gel electrophoresis. ..

    Article Title: Folding complex DNA nanostructures from limited sets of reusable sequences
    Article Snippet: .. For the custom scaffold 24-helix bundle, the purified scaffold was subsequently digested with BtsCI (NEB, Catalog # R0647L) as follows: 10 μL of ssDNA at 100 nM with 2 μL of 10x CutSmart Buffer, 1 μL of BtsCI and 7 μL of ddH2 O was incubated at 50°C overnight. .. Note, that the final scaffold sequence will contain two dsDNA restriction sites for BtsCI (hairpin at each end, Supplementary Figure S2) and several ssDNA restriction sites for BtsCI.

    Article Title: In vitro Assays for Eukaryotic Leading/Lagging Strand DNA Replication
    Article Snippet: .. Bts CI (New England Biolabs, catalog number: R0647L) β-Agarase I (New England Biolabs, catalog number: M0392L) 3 M sodium acetate (CH3 COONa), pH 5.2 (Sigma-Aldrich, catalog number: S2889) Isopropanol (Sigma-Aldrich, catalog number: 190764) Glycogen, molecular biology grade (Thermo Fisher Scientific, Thermo Scientific™, catalog number: R0561) Ethanol (Sigma-Aldrich, catalog number: E7023) 1 µM RFC (Replication Factor C; see for purification details) 5 µM PCNA (Proliferating Cellular Nuclear Antigen; see for purification details) 2 µM Pol ε (see for purification details) 2 µM Pol δ (see for purification details) 2 µM Pol α (see for purification details) 20 µM RPA (Replication Protein A; see for purification details) 32 P-α-dCTP, 3,000 Ci/mmol, 3.3 µM (PerkinElmer, catalog number: BLU013H) 32 P-α-dGTP, 3,000 Ci/mmol, 3.3 µM (PerkinElmer, catalog number: BLU514H) LE agarose (BioExpress, GeneMate, catalog number: E-3120-500) 10 N sodium hydroxide (NaOH) (Fisher Scientific, catalog number: SS255) Glycerol Xylene cylanol Tris-HCl, pH 8.0 Tris base (RPI, catalog number: T60040-500.0) Boric acid (RPI, catalog number: B32050-5000.0) Sodium citrate 1-Butanol Tris-acetate, pH 7.5 Bovine serum albumin (BSA) (New England Biolabs, catalog number: B9000S) Tris(2-carboxyethyl)phosphine (TCEP) pH 7.5 100 mM dithiothreitol (DTT) (Thermo Fisher Scientific, Thermo Scientific™, catalog number: R0861) Potassium glutamate Magnesium acetate 1% SDS 6× gel loading dye (see Recipes) TE buffer, pH 8.0 (see Recipes) 10× TBE (Tris/Borate/EDTA; see Recipes) DNA elution buffer (see Recipes) 20× SSC (see Recipes) 1-Butanol saturated water (see Recipes) 5× TDBG (see Recipes) 10× MK (see Recipes) dA/dC mix (see Recipes) dT/dG mix (see Recipes) T/G/C mix (see Recipes) Stop solution (see Recipes) Alkaline running buffer (see Recipes) .. Heating block ( e.g., VWR, catalog number: 12621-084) Fraction collector ( e.g., Gilson, model: F203B) Variable mode gel imager ( e.g., GE Typhoon) UV-vis spectrophotometer ( e.g., Thermo Fisher Scientific, Thermo Scientific™, model: NanoDrop™ 2000) Vacuum dessicator ( e.g., Thermo Fisher Scientific, Thermo Scientific™, catalog number: 5309-0250) UV light box UV blocking face shield (e.g., Sigma-Aldrich, catalog number: F8142) Note: This product has been discontinued.

    Incubation:

    Article Title: Folding complex DNA nanostructures from limited sets of reusable sequences
    Article Snippet: .. For the custom scaffold 24-helix bundle, the purified scaffold was subsequently digested with BtsCI (NEB, Catalog # R0647L) as follows: 10 μL of ssDNA at 100 nM with 2 μL of 10x CutSmart Buffer, 1 μL of BtsCI and 7 μL of ddH2 O was incubated at 50°C overnight. .. Note, that the final scaffold sequence will contain two dsDNA restriction sites for BtsCI (hairpin at each end, Supplementary Figure S2) and several ssDNA restriction sites for BtsCI.

    Polymerase Chain Reaction:

    Article Title: Restoration of Vision in the pde6?-deficient Dog, a Large Animal Model of Rod-cone Dystrophy
    Article Snippet: .. Pde6β PCR products were purified by Nucleospin Extract II (Macherey-Nagel, Hoerdt, France) before digestion by BtsCI (New England Biolabs, Ipswich, MA) for 2 hours at 50 °C according to the manufacturer's instructions and analyzed by 3.5% agarose gel electrophoresis. ..

    Article Title: Mice expressing a human KATP channel mutation have altered channel ATP sensitivity but no cardiac abnormalities
    Article Snippet: .. PCR products were digested with BtsCI (New England Biolabs, Hitchin, UK) for ∼2 h at 50°C. .. Digested PCR fragments were visualised on an agarose gel with ethidium bromide.

    Recombinase Polymerase Amplification:

    Article Title: In vitro Assays for Eukaryotic Leading/Lagging Strand DNA Replication
    Article Snippet: .. Bts CI (New England Biolabs, catalog number: R0647L) β-Agarase I (New England Biolabs, catalog number: M0392L) 3 M sodium acetate (CH3 COONa), pH 5.2 (Sigma-Aldrich, catalog number: S2889) Isopropanol (Sigma-Aldrich, catalog number: 190764) Glycogen, molecular biology grade (Thermo Fisher Scientific, Thermo Scientific™, catalog number: R0561) Ethanol (Sigma-Aldrich, catalog number: E7023) 1 µM RFC (Replication Factor C; see for purification details) 5 µM PCNA (Proliferating Cellular Nuclear Antigen; see for purification details) 2 µM Pol ε (see for purification details) 2 µM Pol δ (see for purification details) 2 µM Pol α (see for purification details) 20 µM RPA (Replication Protein A; see for purification details) 32 P-α-dCTP, 3,000 Ci/mmol, 3.3 µM (PerkinElmer, catalog number: BLU013H) 32 P-α-dGTP, 3,000 Ci/mmol, 3.3 µM (PerkinElmer, catalog number: BLU514H) LE agarose (BioExpress, GeneMate, catalog number: E-3120-500) 10 N sodium hydroxide (NaOH) (Fisher Scientific, catalog number: SS255) Glycerol Xylene cylanol Tris-HCl, pH 8.0 Tris base (RPI, catalog number: T60040-500.0) Boric acid (RPI, catalog number: B32050-5000.0) Sodium citrate 1-Butanol Tris-acetate, pH 7.5 Bovine serum albumin (BSA) (New England Biolabs, catalog number: B9000S) Tris(2-carboxyethyl)phosphine (TCEP) pH 7.5 100 mM dithiothreitol (DTT) (Thermo Fisher Scientific, Thermo Scientific™, catalog number: R0861) Potassium glutamate Magnesium acetate 1% SDS 6× gel loading dye (see Recipes) TE buffer, pH 8.0 (see Recipes) 10× TBE (Tris/Borate/EDTA; see Recipes) DNA elution buffer (see Recipes) 20× SSC (see Recipes) 1-Butanol saturated water (see Recipes) 5× TDBG (see Recipes) 10× MK (see Recipes) dA/dC mix (see Recipes) dT/dG mix (see Recipes) T/G/C mix (see Recipes) Stop solution (see Recipes) Alkaline running buffer (see Recipes) .. Heating block ( e.g., VWR, catalog number: 12621-084) Fraction collector ( e.g., Gilson, model: F203B) Variable mode gel imager ( e.g., GE Typhoon) UV-vis spectrophotometer ( e.g., Thermo Fisher Scientific, Thermo Scientific™, model: NanoDrop™ 2000) Vacuum dessicator ( e.g., Thermo Fisher Scientific, Thermo Scientific™, catalog number: 5309-0250) UV light box UV blocking face shield (e.g., Sigma-Aldrich, catalog number: F8142) Note: This product has been discontinued.

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    New England Biolabs btsc i
    PCR-RFLP patterns of 26S rDNA PCR digested with restriction enzymes (A) <t>Hha</t> I, (B) <t>BtsC</t> I of 11 Malassezia standard strains in hyperpigmented lesions of 21 patients. Lanes: M: molecular Marker, 1: Malassezia globosa (CBS7966), 2: M. globosa (CBS7966), 3: M. restricta (KCTC7848), 4: M. globosa (CBS7966), 5: M. restricta (KCTC7848), 6: M. restricta (KCTC7848), 7: M. restricta (KCTC7848), 8: M. slooffiae (KCTC17431), 9: M. globosa (CBS7966), 10: M. globosa (CBS7966), 11: M. restricta (KCTC7848), 12: M. globosa (CBS7966), 13: M. globosa (CBS7966), 14: M. furfur (KCTC7743), 15: M. slooffiae (KCTC17431), 16: M. globosa (CBS7966), 17: M. globosa (CBS7966), 18: M. restricta (KCTC7848), 19: M. restricta (KCTC7848), 20: M. furfur (KCTC7743), 21: M. sympodialis (KCTC7985). PCR: polymerase chain reaction, RFLP: restriction fragment length polymorphism.
    Btsc I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/btsc i/product/New England Biolabs
    Average 95 stars, based on 20 article reviews
    Price from $9.99 to $1999.99
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    95/100 stars
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    PCR-RFLP patterns of 26S rDNA PCR digested with restriction enzymes (A) Hha I, (B) BtsC I of 11 Malassezia standard strains in hyperpigmented lesions of 21 patients. Lanes: M: molecular Marker, 1: Malassezia globosa (CBS7966), 2: M. globosa (CBS7966), 3: M. restricta (KCTC7848), 4: M. globosa (CBS7966), 5: M. restricta (KCTC7848), 6: M. restricta (KCTC7848), 7: M. restricta (KCTC7848), 8: M. slooffiae (KCTC17431), 9: M. globosa (CBS7966), 10: M. globosa (CBS7966), 11: M. restricta (KCTC7848), 12: M. globosa (CBS7966), 13: M. globosa (CBS7966), 14: M. furfur (KCTC7743), 15: M. slooffiae (KCTC17431), 16: M. globosa (CBS7966), 17: M. globosa (CBS7966), 18: M. restricta (KCTC7848), 19: M. restricta (KCTC7848), 20: M. furfur (KCTC7743), 21: M. sympodialis (KCTC7985). PCR: polymerase chain reaction, RFLP: restriction fragment length polymorphism.

    Journal: Annals of Dermatology

    Article Title: Skin Characteristics in Patients with Pityriasis Versicolor Using Non-Invasive Method, MPA5

    doi: 10.5021/ad.2012.24.4.444

    Figure Lengend Snippet: PCR-RFLP patterns of 26S rDNA PCR digested with restriction enzymes (A) Hha I, (B) BtsC I of 11 Malassezia standard strains in hyperpigmented lesions of 21 patients. Lanes: M: molecular Marker, 1: Malassezia globosa (CBS7966), 2: M. globosa (CBS7966), 3: M. restricta (KCTC7848), 4: M. globosa (CBS7966), 5: M. restricta (KCTC7848), 6: M. restricta (KCTC7848), 7: M. restricta (KCTC7848), 8: M. slooffiae (KCTC17431), 9: M. globosa (CBS7966), 10: M. globosa (CBS7966), 11: M. restricta (KCTC7848), 12: M. globosa (CBS7966), 13: M. globosa (CBS7966), 14: M. furfur (KCTC7743), 15: M. slooffiae (KCTC17431), 16: M. globosa (CBS7966), 17: M. globosa (CBS7966), 18: M. restricta (KCTC7848), 19: M. restricta (KCTC7848), 20: M. furfur (KCTC7743), 21: M. sympodialis (KCTC7985). PCR: polymerase chain reaction, RFLP: restriction fragment length polymorphism.

    Article Snippet: Two restriction enzymes, Hha I (Koschem, Seoul, Korea) and BtsC I (NEB, London, England) were used to perform 26S rDNA-RFLP of Malassezia .

    Techniques: Polymerase Chain Reaction, Marker

    PCR-RFLP patterns of 26S rDNA PCR digested with restriction enzymes (A) Hha I, (B) BtsC I of 11 Malassezia standard strains in hypopigmented lesions of 9 patients. Lanes: M: molecular Marker , 1: Malassezia slooffiae (KCTC17431), 2: M. globosa (CBS7966), 3: M. restricta (KCTC7848), 4: M. restricta (KCTC7848), 5: M. globosa (CBS7966), 6: M. globosa (CBS7966), 7: M. globosa (CBS7966), 8: M. sympodialis (KCTC7985), 9: M. furfur (KCTC7743). PCR: polymerase chain reaction, RFLP: restriction fragment length polymorphism.

    Journal: Annals of Dermatology

    Article Title: Skin Characteristics in Patients with Pityriasis Versicolor Using Non-Invasive Method, MPA5

    doi: 10.5021/ad.2012.24.4.444

    Figure Lengend Snippet: PCR-RFLP patterns of 26S rDNA PCR digested with restriction enzymes (A) Hha I, (B) BtsC I of 11 Malassezia standard strains in hypopigmented lesions of 9 patients. Lanes: M: molecular Marker , 1: Malassezia slooffiae (KCTC17431), 2: M. globosa (CBS7966), 3: M. restricta (KCTC7848), 4: M. restricta (KCTC7848), 5: M. globosa (CBS7966), 6: M. globosa (CBS7966), 7: M. globosa (CBS7966), 8: M. sympodialis (KCTC7985), 9: M. furfur (KCTC7743). PCR: polymerase chain reaction, RFLP: restriction fragment length polymorphism.

    Article Snippet: Two restriction enzymes, Hha I (Koschem, Seoul, Korea) and BtsC I (NEB, London, England) were used to perform 26S rDNA-RFLP of Malassezia .

    Techniques: Polymerase Chain Reaction, Marker

    Kcnj11 expression in the heart. a Kcnj11 expression in tissue isolated from ROSA, WT, Mck-Cre and m-V59M mice. WT, but not mutant (V59M), Kcnj11 cDNA is cut by the restriction enzyme BtsCI: the presence of two bands thus indicates the presence of the WT gene only and three bands indicates both WT and mutant genes. Data are representative of experiments on seven ROSA, four m-V59M, four WT and four Mck-Cre mice. sk1, quadriceps muscle; sk2, triceps muscle; sk3, diaphragm. b,c Quantitative PCR showing expression of Kcnj11 in heart and brain ( b ) and Gfp in heart ( c ) of control (grey bars, n = 5) and m-V59M mice (white bars, n = 5), relative to a panel of housekeeping genes. * p

    Journal: Diabetologia

    Article Title: Mice expressing a human KATP channel mutation have altered channel ATP sensitivity but no cardiac abnormalities

    doi: 10.1007/s00125-011-2428-6

    Figure Lengend Snippet: Kcnj11 expression in the heart. a Kcnj11 expression in tissue isolated from ROSA, WT, Mck-Cre and m-V59M mice. WT, but not mutant (V59M), Kcnj11 cDNA is cut by the restriction enzyme BtsCI: the presence of two bands thus indicates the presence of the WT gene only and three bands indicates both WT and mutant genes. Data are representative of experiments on seven ROSA, four m-V59M, four WT and four Mck-Cre mice. sk1, quadriceps muscle; sk2, triceps muscle; sk3, diaphragm. b,c Quantitative PCR showing expression of Kcnj11 in heart and brain ( b ) and Gfp in heart ( c ) of control (grey bars, n = 5) and m-V59M mice (white bars, n = 5), relative to a panel of housekeeping genes. * p

    Article Snippet: PCR products were digested with BtsCI (New England Biolabs, Hitchin, UK) for ∼2 h at 50°C.

    Techniques: Expressing, Isolation, Mouse Assay, Mutagenesis, Real-time Polymerase Chain Reaction

    Dominance of the inoculated strains after SSF as revealed by their RFLP profiles. The RFLP profiles from the 16S rRNA genes of the selected strains (S7, CR18 and T5) were generated using a culture-independent approach. DNA was extracted directly from FSBM (inoculated and non-Inoculated groups), and the PCR amplicons were digested with the restriction enzymes Bmt I and BtsC I. The figure shows the RFLPs of the selected strains (S7, CR18 and T5) and the RFLP profiles from five replicates of inoculated and non-inoculated SSFs. The arrows indicate the presence of the S7 and T5 strains as dominant in the fermentation process.

    Journal: PLoS ONE

    Article Title: Reduction of Soybean Meal Non-Starch Polysaccharides and ?-Galactosides by Solid-State Fermentation Using Cellulolytic Bacteria Obtained from Different Environments

    doi: 10.1371/journal.pone.0044783

    Figure Lengend Snippet: Dominance of the inoculated strains after SSF as revealed by their RFLP profiles. The RFLP profiles from the 16S rRNA genes of the selected strains (S7, CR18 and T5) were generated using a culture-independent approach. DNA was extracted directly from FSBM (inoculated and non-Inoculated groups), and the PCR amplicons were digested with the restriction enzymes Bmt I and BtsC I. The figure shows the RFLPs of the selected strains (S7, CR18 and T5) and the RFLP profiles from five replicates of inoculated and non-inoculated SSFs. The arrows indicate the presence of the S7 and T5 strains as dominant in the fermentation process.

    Article Snippet: The amplicons were then digested by the restriction enzymes Bmt I and BtsC I (New England BioLabs).

    Techniques: Generated, Polymerase Chain Reaction