btsci restriction enzyme  (New England Biolabs)


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    Structured Review

    New England Biolabs btsci restriction enzyme
    Btsci Restriction Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/btsci restriction enzyme/product/New England Biolabs
    Average 90 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    btsci restriction enzyme - by Bioz Stars, 2020-03
    90/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Programming molecular topologies from single-stranded nucleic acids
    Article Snippet: Topological control experiments The Biobasic company (Biobasic.com) synthesized four ssDNAs with the customized sequences and then cloned these sequences into a pBluescript SK( + ) vector (Biobasic), with the gene sequences flanked by two BtsCI restriction sites. .. The ssDNAs were cleaved off from the recombinant phage DNAs by using a BtsCI restriction enzyme (New England Biolabs), and were gel purified by using a 4% urea denaturing PAGE gel.

    Amplification:

    Article Title: Quinolone resistance mechanisms among third-generation cephalosporin resistant isolates of Enterobacter spp. in a Bulgarian university hospital
    Article Snippet: Plasmid mediated quinolone resistant determinants qnrA, qnrB, qnrC, qnr D, qnrS, and qepA were amplified and sequenced with ABI 3130xl Genetic Analyzer. .. The exact allele of aac(6ʹ)-Ib enzymes was determined with restriction with BtsCI (New England Biolabs, UK).

    Article Title: Restoration of Vision in the pde6?-deficient Dog, a Large Animal Model of Rod-cone Dystrophy
    Article Snippet: For pde6β, conditions of amplification were: an initial denaturation step at 95 °C for 5 minutes, followed by 10 cycles at 94 °C for 30 seconds, 60 °C for 30 seconds, 72 °C for 30 seconds, 30 cycles at 94 °C for 30 seconds, 56 °C for 30 seconds, 72 °C for 30 seconds and a final incubation step at 72 °C for 7 minutes. .. Pde6β PCR products were purified by Nucleospin Extract II (Macherey-Nagel, Hoerdt, France) before digestion by BtsCI (New England Biolabs, Ipswich, MA) for 2 hours at 50 °C according to the manufacturer's instructions and analyzed by 3.5% agarose gel electrophoresis.

    Article Title: SIAE Rare Variants in Juvenile Idiopathic Arthritis and Primary Antibody Deficiencies
    Article Snippet: .. In particular, for the detection of p.Q343P mutation, a 360 bp fragment (encompassing exon 8) was amplified by PCR and subjected to BtsCI overnight digestion (New England Biolabs, Ipswich, UK) at 50°C. ..

    Article Title: Folding complex DNA nanostructures from limited sets of reusable sequences
    Article Snippet: Paragraph title: Scaffold amplification and purification ... For the custom scaffold 24-helix bundle, the purified scaffold was subsequently digested with BtsCI (NEB, Catalog # R0647L) as follows: 10 μL of ssDNA at 100 nM with 2 μL of 10x CutSmart Buffer, 1 μL of BtsCI and 7 μL of ddH2 O was incubated at 50°C overnight.

    Article Title: Lack of effect of apolipoprotein C3 polymorphisms on indices of liver steatosis, lipid profile and insulin resistance in obese Southern Europeans
    Article Snippet: .. A 497-bp fragment was amplified and enzymatically restricted using BtsCI (New England Biolabs, Ipswich, MA, USA). .. The resulted enzyme-digested fragments of the PCR products were fractionated on 3.5% agarose gels, stained with ethidium bromide, and visualized with an imaging system (Amersham-Pharmacia Biosciences).

    Article Title: Skin Characteristics in Patients with Pityriasis Versicolor Using Non-Invasive Method, MPA5
    Article Snippet: After the confirmation of the amplified 26S rDNA, PCR (Veriti® 96-Well Fast Thermal Cycler. .. Two restriction enzymes, Hha I (Koschem, Seoul, Korea) and BtsC I (NEB, London, England) were used to perform 26S rDNA-RFLP of Malassezia .

    Article Title: Analysis of quinolone-resistance in commensal and diarrheagenic Escherichia coli isolates from infants in Lima, Peru
    Article Snippet: The a ac(6′)-Ib gene was amplified as previously described. .. Positive PCR products were digested with BtsC I (New England Biolabs, Beijing, China) to identify the aac-(6′)-Ib-cr variant.

    Article Title: Chromosomal and plasmid-mediated fluoroquinolone resistance mechanisms among broad-spectrum-cephalosporin-resistant Escherichia coli isolates recovered from companion animals in the USA
    Article Snippet: All E. coli isolates that tested positive for aac(6 ′ )-Ib-cr were confirmed by enzymatic digestion with BtsCI restriction endonuclease (New England Biolabs, Ipswich, MA, USA) or by DNA sequencing. .. PCR amplification and DNA sequencing of the quinolone resistance-determining regions of gyrA , gyrB , parC and parE .

    Article Title: Reduction of Soybean Meal Non-Starch Polysaccharides and ?-Galactosides by Solid-State Fermentation Using Cellulolytic Bacteria Obtained from Different Environments
    Article Snippet: The amplicons were then digested by the restriction enzymes Bmt I and BtsC I (New England BioLabs). .. The RFLP reaction was performed using 1 U Bmt I and 2 U BtsC I , 1.0 µl of NEB buffer 2, and 1.0 µl amplified DNA in a 10-µl reaction volume.

    Article Title: Mice expressing a human KATP channel mutation have altered channel ATP sensitivity but no cardiac abnormalities
    Article Snippet: Semi-quantitative PCR Mouse Kcnj11 transcript was amplified by PCR using cDNA prepared from muscle and brain tissues from m-V59M and control mice, as previously described [ ]. .. PCR products were digested with BtsCI (New England Biolabs, Hitchin, UK) for ∼2 h at 50°C.

    Article Title: Expression of an activating mutation in the gene encoding the KATP channel subunit Kir6.2 in mouse pancreatic ? cells recapitulates neonatal diabetes
    Article Snippet: Mouse Kir6.2 transcript was amplified by PCR using cDNA prepared from islets isolated from either V59M or WT 5-week-old mice. .. After 40 cycles (95°C for 30 seconds; 60°C for 30 seconds; 72°C for 1 minute), PCR products were digested with BtsCI restriction enzyme (New England BioLabs) for approximately 1 hour at 50°C and digested PCR fragments visualized on a 2% agarose gel.

    Positive Control:

    Article Title: Chromosomal and plasmid-mediated fluoroquinolone resistance mechanisms among broad-spectrum-cephalosporin-resistant Escherichia coli isolates recovered from companion animals in the USA
    Article Snippet: The qnr -positive, aac(6′)-Ib-cr -positive and qepA -positive control strains, E. coli J53 pMG252 ( qnrA1 ), J53 pMG298 [ qnrB1 and aac(6′)-Ib-cr ], J53 pMG306 ( qnrS1 ), J53 p2007057 ( qnrD ) and J53 pAT851 ( qepA ) and Proteus mirabilis 06-498 ( qnrC ), were provided by Dr George Jacoby (Lahey Clinic Medical Center, Burlington, MA, USA). .. All E. coli isolates that tested positive for aac(6 ′ )-Ib-cr were confirmed by enzymatic digestion with BtsCI restriction endonuclease (New England Biolabs, Ipswich, MA, USA) or by DNA sequencing.

    Synthesized:

    Article Title: Programming molecular topologies from single-stranded nucleic acids
    Article Snippet: Topological control experiments The Biobasic company (Biobasic.com) synthesized four ssDNAs with the customized sequences and then cloned these sequences into a pBluescript SK( + ) vector (Biobasic), with the gene sequences flanked by two BtsCI restriction sites. .. The ssDNAs were cleaved off from the recombinant phage DNAs by using a BtsCI restriction enzyme (New England Biolabs), and were gel purified by using a 4% urea denaturing PAGE gel.

    Article Title: In vitro Assays for Eukaryotic Leading/Lagging Strand DNA Replication
    Article Snippet: Razor blade 1.57 mm OD polyethylene tubing ( e.g ., Clay Adams® Intramedic® , BD, catalog number: 427431) Sephadex microcentrifuge columns (Illustra Microspin G-25) (GE Healthcare, catalog number: 27-5325-01) Plastic wrap ( e.g. , Fisherbrand Clear Plastic Wrap, Fisher Scientific, catalog number: 22-305654) C-fold paper towels ( e.g. , Scott paper towels, KCWW, Kimberly-Clark, catalog number: 01510) Positively charged nylon DNA blotting membrane (Hybond-N+, 30.0×50.0 cm) (GE Healthcare, catalog number: RPN3050B) Chromatography transfer paper (Whatman 3MM, 46.0×57.0 cm) (GE Healthcare, catalog number: 3030-917) Syringe tip ( e.g ., B-D 18 G 1 ½ PrecisionGlide® Needle) (BD, catalog number: 305196) phiX174 virion DNA, 1 mg/ml (New England Biolabs, catalog number: N3023L) Phi29 DNA polymerase (New England Biolabs, catalog number: M0269S) 100 mM dNTP (deoxynucleotide triphosphate) set (Thermo Fisher Scientific, Thermo Scientific™, catalog number: R0181) 1 µM CMG ( C dc45 M cm2–7 G ins) helicase (see for purification details) pUC19, 1 mg/ml (New England Biolabs, catalog number: N3041L) Bsa I-HF with CutSmart buffer (New England Biolabs, catalog number: R3535L) ‘blockLd’ oligo* ‘blockLg’ oligo* ‘Pr1B’ oligo* ‘160Ld’ oligo* ‘91Lg’ oligo* ‘Fork primer’ oligo* Nucleotide-biased template (synthesized by Biomatik, Wilmington DE)* *Note: See . .. Bts CI (New England Biolabs, catalog number: R0647L) β-Agarase I (New England Biolabs, catalog number: M0392L) 3 M sodium acetate (CH3 COONa), pH 5.2 (Sigma-Aldrich, catalog number: S2889) Isopropanol (Sigma-Aldrich, catalog number: 190764) Glycogen, molecular biology grade (Thermo Fisher Scientific, Thermo Scientific™, catalog number: R0561) Ethanol (Sigma-Aldrich, catalog number: E7023) 1 µM RFC (Replication Factor C; see for purification details) 5 µM PCNA (Proliferating Cellular Nuclear Antigen; see for purification details) 2 µM Pol ε (see for purification details) 2 µM Pol δ (see for purification details) 2 µM Pol α (see for purification details) 20 µM RPA (Replication Protein A; see for purification details) 32 P-α-dCTP, 3,000 Ci/mmol, 3.3 µM (PerkinElmer, catalog number: BLU013H) 32 P-α-dGTP, 3,000 Ci/mmol, 3.3 µM (PerkinElmer, catalog number: BLU514H) LE agarose (BioExpress, GeneMate, catalog number: E-3120-500) 10 N sodium hydroxide (NaOH) (Fisher Scientific, catalog number: SS255) Glycerol Xylene cylanol Tris-HCl, pH 8.0 Tris base (RPI, catalog number: T60040-500.0) Boric acid (RPI, catalog number: B32050-5000.0) Sodium citrate 1-Butanol Tris-acetate, pH 7.5 Bovine serum albumin (BSA) (New England Biolabs, catalog number: B9000S) Tris(2-carboxyethyl)phosphine (TCEP) pH 7.5 100 mM dithiothreitol (DTT) (Thermo Fisher Scientific, Thermo Scientific™, catalog number: R0861) Potassium glutamate Magnesium acetate 1% SDS 6× gel loading dye (see Recipes) TE buffer, pH 8.0 (see Recipes) 10× TBE (Tris/Borate/EDTA; see Recipes) DNA elution buffer (see Recipes) 20× SSC (see Recipes) 1-Butanol saturated water (see Recipes) 5× TDBG (see Recipes) 10× MK (see Recipes) dA/dC mix (see Recipes) dT/dG mix (see Recipes) T/G/C mix (see Recipes) Stop solution (see Recipes) Alkaline running buffer (see Recipes)

    Real-time Polymerase Chain Reaction:

    Article Title: Lack of effect of apolipoprotein C3 polymorphisms on indices of liver steatosis, lipid profile and insulin resistance in obese Southern Europeans
    Article Snippet: The assay was performed using the TaqMan C7241_10 assay (Applied Biosystems, Foster City, CA) in a total volume of 2.7 μl on a 7900HT Fast Real-Time PCR instrument (Applied Biosystems). .. A 497-bp fragment was amplified and enzymatically restricted using BtsCI (New England Biolabs, Ipswich, MA, USA).

    Incubation:

    Article Title: Restoration of Vision in the pde6?-deficient Dog, a Large Animal Model of Rod-cone Dystrophy
    Article Snippet: For pde6β, conditions of amplification were: an initial denaturation step at 95 °C for 5 minutes, followed by 10 cycles at 94 °C for 30 seconds, 60 °C for 30 seconds, 72 °C for 30 seconds, 30 cycles at 94 °C for 30 seconds, 56 °C for 30 seconds, 72 °C for 30 seconds and a final incubation step at 72 °C for 7 minutes. .. Pde6β PCR products were purified by Nucleospin Extract II (Macherey-Nagel, Hoerdt, France) before digestion by BtsCI (New England Biolabs, Ipswich, MA) for 2 hours at 50 °C according to the manufacturer's instructions and analyzed by 3.5% agarose gel electrophoresis.

    Article Title: Folding complex DNA nanostructures from limited sets of reusable sequences
    Article Snippet: .. For the custom scaffold 24-helix bundle, the purified scaffold was subsequently digested with BtsCI (NEB, Catalog # R0647L) as follows: 10 μL of ssDNA at 100 nM with 2 μL of 10x CutSmart Buffer, 1 μL of BtsCI and 7 μL of ddH2 O was incubated at 50°C overnight. .. Note, that the final scaffold sequence will contain two dsDNA restriction sites for BtsCI (hairpin at each end, Supplementary Figure S2) and several ssDNA restriction sites for BtsCI.

    Article Title: Reduction of Soybean Meal Non-Starch Polysaccharides and ?-Galactosides by Solid-State Fermentation Using Cellulolytic Bacteria Obtained from Different Environments
    Article Snippet: The amplicons were then digested by the restriction enzymes Bmt I and BtsC I (New England BioLabs). .. The digestion process consisted of a 3 h incubation at 50°C, then 2 h at 37°C and 1 h at 37°C with 1 µl of Proteinase K.

    Recombinase Polymerase Amplification:

    Article Title: In vitro Assays for Eukaryotic Leading/Lagging Strand DNA Replication
    Article Snippet: .. Bts CI (New England Biolabs, catalog number: R0647L) β-Agarase I (New England Biolabs, catalog number: M0392L) 3 M sodium acetate (CH3 COONa), pH 5.2 (Sigma-Aldrich, catalog number: S2889) Isopropanol (Sigma-Aldrich, catalog number: 190764) Glycogen, molecular biology grade (Thermo Fisher Scientific, Thermo Scientific™, catalog number: R0561) Ethanol (Sigma-Aldrich, catalog number: E7023) 1 µM RFC (Replication Factor C; see for purification details) 5 µM PCNA (Proliferating Cellular Nuclear Antigen; see for purification details) 2 µM Pol ε (see for purification details) 2 µM Pol δ (see for purification details) 2 µM Pol α (see for purification details) 20 µM RPA (Replication Protein A; see for purification details) 32 P-α-dCTP, 3,000 Ci/mmol, 3.3 µM (PerkinElmer, catalog number: BLU013H) 32 P-α-dGTP, 3,000 Ci/mmol, 3.3 µM (PerkinElmer, catalog number: BLU514H) LE agarose (BioExpress, GeneMate, catalog number: E-3120-500) 10 N sodium hydroxide (NaOH) (Fisher Scientific, catalog number: SS255) Glycerol Xylene cylanol Tris-HCl, pH 8.0 Tris base (RPI, catalog number: T60040-500.0) Boric acid (RPI, catalog number: B32050-5000.0) Sodium citrate 1-Butanol Tris-acetate, pH 7.5 Bovine serum albumin (BSA) (New England Biolabs, catalog number: B9000S) Tris(2-carboxyethyl)phosphine (TCEP) pH 7.5 100 mM dithiothreitol (DTT) (Thermo Fisher Scientific, Thermo Scientific™, catalog number: R0861) Potassium glutamate Magnesium acetate 1% SDS 6× gel loading dye (see Recipes) TE buffer, pH 8.0 (see Recipes) 10× TBE (Tris/Borate/EDTA; see Recipes) DNA elution buffer (see Recipes) 20× SSC (see Recipes) 1-Butanol saturated water (see Recipes) 5× TDBG (see Recipes) 10× MK (see Recipes) dA/dC mix (see Recipes) dT/dG mix (see Recipes) T/G/C mix (see Recipes) Stop solution (see Recipes) Alkaline running buffer (see Recipes) .. Heating block ( e.g., VWR, catalog number: 12621-084) Fraction collector ( e.g., Gilson, model: F203B) Variable mode gel imager ( e.g., GE Typhoon) UV-vis spectrophotometer ( e.g., Thermo Fisher Scientific, Thermo Scientific™, model: NanoDrop™ 2000) Vacuum dessicator ( e.g., Thermo Fisher Scientific, Thermo Scientific™, catalog number: 5309-0250) UV light box UV blocking face shield (e.g., Sigma-Aldrich, catalog number: F8142) Note: This product has been discontinued.

    Hybridization:

    Article Title: Nanoswitch-linked immunosorbent assay (NLISA) for fast, sensitive, and specific protein detection
    Article Snippet: Briefly, circular ssDNA from the 7,249-nt bacteriophage M13 (New England BioLabs) was linearized by enzymatic cleavage of a single site using BtscI (New England BioLabs) and a site-specific oligonucleotide. .. This ssDNA scaffold was mixed with a molar excess of tiling 60-mer oligonucleotides (10:1), excluding the complementary regions for antibody hybridization, and subjected to a temperature ramp from 90 to 20 °C at 1 °C⋅min−1 .

    Chromatography:

    Article Title: In vitro Assays for Eukaryotic Leading/Lagging Strand DNA Replication
    Article Snippet: Razor blade 1.57 mm OD polyethylene tubing ( e.g ., Clay Adams® Intramedic® , BD, catalog number: 427431) Sephadex microcentrifuge columns (Illustra Microspin G-25) (GE Healthcare, catalog number: 27-5325-01) Plastic wrap ( e.g. , Fisherbrand Clear Plastic Wrap, Fisher Scientific, catalog number: 22-305654) C-fold paper towels ( e.g. , Scott paper towels, KCWW, Kimberly-Clark, catalog number: 01510) Positively charged nylon DNA blotting membrane (Hybond-N+, 30.0×50.0 cm) (GE Healthcare, catalog number: RPN3050B) Chromatography transfer paper (Whatman 3MM, 46.0×57.0 cm) (GE Healthcare, catalog number: 3030-917) Syringe tip ( e.g ., B-D 18 G 1 ½ PrecisionGlide® Needle) (BD, catalog number: 305196) phiX174 virion DNA, 1 mg/ml (New England Biolabs, catalog number: N3023L) Phi29 DNA polymerase (New England Biolabs, catalog number: M0269S) 100 mM dNTP (deoxynucleotide triphosphate) set (Thermo Fisher Scientific, Thermo Scientific™, catalog number: R0181) 1 µM CMG ( C dc45 M cm2–7 G ins) helicase (see for purification details) pUC19, 1 mg/ml (New England Biolabs, catalog number: N3041L) Bsa I-HF with CutSmart buffer (New England Biolabs, catalog number: R3535L) ‘blockLd’ oligo* ‘blockLg’ oligo* ‘Pr1B’ oligo* ‘160Ld’ oligo* ‘91Lg’ oligo* ‘Fork primer’ oligo* Nucleotide-biased template (synthesized by Biomatik, Wilmington DE)* *Note: See . .. Bts CI (New England Biolabs, catalog number: R0647L) β-Agarase I (New England Biolabs, catalog number: M0392L) 3 M sodium acetate (CH3 COONa), pH 5.2 (Sigma-Aldrich, catalog number: S2889) Isopropanol (Sigma-Aldrich, catalog number: 190764) Glycogen, molecular biology grade (Thermo Fisher Scientific, Thermo Scientific™, catalog number: R0561) Ethanol (Sigma-Aldrich, catalog number: E7023) 1 µM RFC (Replication Factor C; see for purification details) 5 µM PCNA (Proliferating Cellular Nuclear Antigen; see for purification details) 2 µM Pol ε (see for purification details) 2 µM Pol δ (see for purification details) 2 µM Pol α (see for purification details) 20 µM RPA (Replication Protein A; see for purification details) 32 P-α-dCTP, 3,000 Ci/mmol, 3.3 µM (PerkinElmer, catalog number: BLU013H) 32 P-α-dGTP, 3,000 Ci/mmol, 3.3 µM (PerkinElmer, catalog number: BLU514H) LE agarose (BioExpress, GeneMate, catalog number: E-3120-500) 10 N sodium hydroxide (NaOH) (Fisher Scientific, catalog number: SS255) Glycerol Xylene cylanol Tris-HCl, pH 8.0 Tris base (RPI, catalog number: T60040-500.0) Boric acid (RPI, catalog number: B32050-5000.0) Sodium citrate 1-Butanol Tris-acetate, pH 7.5 Bovine serum albumin (BSA) (New England Biolabs, catalog number: B9000S) Tris(2-carboxyethyl)phosphine (TCEP) pH 7.5 100 mM dithiothreitol (DTT) (Thermo Fisher Scientific, Thermo Scientific™, catalog number: R0861) Potassium glutamate Magnesium acetate 1% SDS 6× gel loading dye (see Recipes) TE buffer, pH 8.0 (see Recipes) 10× TBE (Tris/Borate/EDTA; see Recipes) DNA elution buffer (see Recipes) 20× SSC (see Recipes) 1-Butanol saturated water (see Recipes) 5× TDBG (see Recipes) 10× MK (see Recipes) dA/dC mix (see Recipes) dT/dG mix (see Recipes) T/G/C mix (see Recipes) Stop solution (see Recipes) Alkaline running buffer (see Recipes)

    Ligation:

    Article Title: Programming molecular topologies from single-stranded nucleic acids
    Article Snippet: The ssDNAs were cleaved off from the recombinant phage DNAs by using a BtsCI restriction enzyme (New England Biolabs), and were gel purified by using a 4% urea denaturing PAGE gel. .. The sticky ends on the two sets of the dsDNAs were able to close the ring structure without ligation.

    RFLP Assay:

    Article Title: Reduction of Soybean Meal Non-Starch Polysaccharides and ?-Galactosides by Solid-State Fermentation Using Cellulolytic Bacteria Obtained from Different Environments
    Article Snippet: Dominant Strains after SSF To assess the persistence and dominance of the inoculated strains at the end of the fermentation process, a specific restriction fragment length polymorphism (RFLP) assay was designed. .. The amplicons were then digested by the restriction enzymes Bmt I and BtsC I (New England BioLabs).

    DNA Sequencing:

    Article Title: Chromosomal and plasmid-mediated fluoroquinolone resistance mechanisms among broad-spectrum-cephalosporin-resistant Escherichia coli isolates recovered from companion animals in the USA
    Article Snippet: .. All E. coli isolates that tested positive for aac(6 ′ )-Ib-cr were confirmed by enzymatic digestion with BtsCI restriction endonuclease (New England Biolabs, Ipswich, MA, USA) or by DNA sequencing. .. The PCR products for qepA and qnrS genes were sequenced.

    Sequencing:

    Article Title: Quinolone resistance mechanisms among third-generation cephalosporin resistant isolates of Enterobacter spp. in a Bulgarian university hospital
    Article Snippet: The exact allele of aac(6ʹ)-Ib enzymes was determined with restriction with BtsCI (New England Biolabs, UK). .. Mutations in QRDR of gyr A and par C were identified with comparison with DNA sequence of QRDR regions of E. cloacae ATCC 13047 (GenBank accession numbers D88980 and D88981 for gyrA and parC, respectively).

    Article Title: SIAE Rare Variants in Juvenile Idiopathic Arthritis and Primary Antibody Deficiencies
    Article Snippet: Molecular Analyses Peripheral blood samples were collected in K3-EDTA tubes from subjects during routine clinical visits; genomic DNA was isolated from samples using the QIAamp DNA Blood Mini Kit (Qiagen, Crawley, United Kingdom), according to manufacturers' instructions, and SIAE variants were detected by direct sequencing after polymerase chain reaction (PCR) amplification of all 10 exons and the exon-intron boundaries, as it has previously been described [ ]. .. In particular, for the detection of p.Q343P mutation, a 360 bp fragment (encompassing exon 8) was amplified by PCR and subjected to BtsCI overnight digestion (New England Biolabs, Ipswich, UK) at 50°C.

    Article Title: Folding complex DNA nanostructures from limited sets of reusable sequences
    Article Snippet: For the custom scaffold 24-helix bundle, the purified scaffold was subsequently digested with BtsCI (NEB, Catalog # R0647L) as follows: 10 μL of ssDNA at 100 nM with 2 μL of 10x CutSmart Buffer, 1 μL of BtsCI and 7 μL of ddH2 O was incubated at 50°C overnight. .. Note, that the final scaffold sequence will contain two dsDNA restriction sites for BtsCI (hairpin at each end, Supplementary Figure S2) and several ssDNA restriction sites for BtsCI.

    Article Title: Chromosomal and plasmid-mediated fluoroquinolone resistance mechanisms among broad-spectrum-cephalosporin-resistant Escherichia coli isolates recovered from companion animals in the USA
    Article Snippet: Paragraph title: Detection of resistance genes and DNA sequence analysis ... All E. coli isolates that tested positive for aac(6 ′ )-Ib-cr were confirmed by enzymatic digestion with BtsCI restriction endonuclease (New England Biolabs, Ipswich, MA, USA) or by DNA sequencing.

    Article Title: Repurposing a benchtop centrifuge for high-throughput single-molecule force spectroscopy
    Article Snippet: Synthetics oligonucleotide: (Sequence: 5′-CTACTAATAGTAGTAGCATTAACATCCAATAAATCATACA-3′). .. BtsCI restriction enzyme (New England Biolabs, R0647S).

    Injection:

    Article Title: Restoration of Vision in the pde6?-deficient Dog, a Large Animal Model of Rod-cone Dystrophy
    Article Snippet: Pde6β PCR products were purified by Nucleospin Extract II (Macherey-Nagel, Hoerdt, France) before digestion by BtsCI (New England Biolabs, Ipswich, MA) for 2 hours at 50 °C according to the manufacturer's instructions and analyzed by 3.5% agarose gel electrophoresis. .. Dogs were anesthetized by intravenous injection of xylazine (BayerHealth Care, Shawnee Mission, KS) and ketamine (Rhone Merieux).

    Recombinant:

    Article Title: Programming molecular topologies from single-stranded nucleic acids
    Article Snippet: .. The ssDNAs were cleaved off from the recombinant phage DNAs by using a BtsCI restriction enzyme (New England Biolabs), and were gel purified by using a 4% urea denaturing PAGE gel. ..

    Transmission Electron Microscopy:

    Article Title: Chromosomal and plasmid-mediated fluoroquinolone resistance mechanisms among broad-spectrum-cephalosporin-resistant Escherichia coli isolates recovered from companion animals in the USA
    Article Snippet: All 54 E. coli isolates were screened for PMQR genes [ qnrA , qnrB , qnrC , qnrD , qnrS , aac(6 ′ )-Ib-cr or qepA ] and β-lactamase genes ( bla TEM , bla CMY-2 and bla CTX-M-1 ). .. All E. coli isolates that tested positive for aac(6 ′ )-Ib-cr were confirmed by enzymatic digestion with BtsCI restriction endonuclease (New England Biolabs, Ipswich, MA, USA) or by DNA sequencing.

    Imaging:

    Article Title: Programming molecular topologies from single-stranded nucleic acids
    Article Snippet: The ssDNAs were cleaved off from the recombinant phage DNAs by using a BtsCI restriction enzyme (New England Biolabs), and were gel purified by using a 4% urea denaturing PAGE gel. .. The two linear dsDNAs were mixed in a 20 nM concentration in a 1× TAE-Mg buffer, annealed from 65 °C to 25 °C at 1 °C per 20 min to form the desired paranemic cohesion interactions, and were then characterized by AFM imaging.

    Article Title: Lack of effect of apolipoprotein C3 polymorphisms on indices of liver steatosis, lipid profile and insulin resistance in obese Southern Europeans
    Article Snippet: A 497-bp fragment was amplified and enzymatically restricted using BtsCI (New England Biolabs, Ipswich, MA, USA). .. The resulted enzyme-digested fragments of the PCR products were fractionated on 3.5% agarose gels, stained with ethidium bromide, and visualized with an imaging system (Amersham-Pharmacia Biosciences).

    DNA Extraction:

    Article Title: Reduction of Soybean Meal Non-Starch Polysaccharides and ?-Galactosides by Solid-State Fermentation Using Cellulolytic Bacteria Obtained from Different Environments
    Article Snippet: Genomic DNA from the inoculated and non-inoculated fermentation vessels was isolated using the PowerSoil DNA isolation kit (Mo Bio). .. The amplicons were then digested by the restriction enzymes Bmt I and BtsC I (New England BioLabs).

    Mutagenesis:

    Article Title: SIAE Rare Variants in Juvenile Idiopathic Arthritis and Primary Antibody Deficiencies
    Article Snippet: .. In particular, for the detection of p.Q343P mutation, a 360 bp fragment (encompassing exon 8) was amplified by PCR and subjected to BtsCI overnight digestion (New England Biolabs, Ipswich, UK) at 50°C. ..

    Isolation:

    Article Title: SIAE Rare Variants in Juvenile Idiopathic Arthritis and Primary Antibody Deficiencies
    Article Snippet: Molecular Analyses Peripheral blood samples were collected in K3-EDTA tubes from subjects during routine clinical visits; genomic DNA was isolated from samples using the QIAamp DNA Blood Mini Kit (Qiagen, Crawley, United Kingdom), according to manufacturers' instructions, and SIAE variants were detected by direct sequencing after polymerase chain reaction (PCR) amplification of all 10 exons and the exon-intron boundaries, as it has previously been described [ ]. .. In particular, for the detection of p.Q343P mutation, a 360 bp fragment (encompassing exon 8) was amplified by PCR and subjected to BtsCI overnight digestion (New England Biolabs, Ipswich, UK) at 50°C.

    Article Title: Reduction of Soybean Meal Non-Starch Polysaccharides and ?-Galactosides by Solid-State Fermentation Using Cellulolytic Bacteria Obtained from Different Environments
    Article Snippet: Genomic DNA from the inoculated and non-inoculated fermentation vessels was isolated using the PowerSoil DNA isolation kit (Mo Bio). .. The amplicons were then digested by the restriction enzymes Bmt I and BtsC I (New England BioLabs).

    Article Title: Expression of an activating mutation in the gene encoding the KATP channel subunit Kir6.2 in mouse pancreatic ? cells recapitulates neonatal diabetes
    Article Snippet: Mouse Kir6.2 transcript was amplified by PCR using cDNA prepared from islets isolated from either V59M or WT 5-week-old mice. .. After 40 cycles (95°C for 30 seconds; 60°C for 30 seconds; 72°C for 1 minute), PCR products were digested with BtsCI restriction enzyme (New England BioLabs) for approximately 1 hour at 50°C and digested PCR fragments visualized on a 2% agarose gel.

    Size-exclusion Chromatography:

    Article Title: In vitro Assays for Eukaryotic Leading/Lagging Strand DNA Replication
    Article Snippet: T4 ligase, including 10× ligase buffer (New England Biolabs, catalog number: M0202M) 100 mM ATP (GE Healthcare, catalog number: 27-2056-01) 0.5 M EDTA, disodium salt (Sigma-Aldrich, catalog number: E5134) 5 M NaCl (Sigma-Aldrich, catalog number: S9888) Sepharose 4B size exclusion chromatography resin (GE Healthcare, catalog number: 17012001) 1 kb MW marker (New England Biolabs, catalog number: N3232L) Ethidium bromide (EthBr, 10 mg/ml) (Thermo Fisher Scientific, Invitrogen™, catalog number: 15585011) T4 kinase and 10× T4 kinase buffer (New England Biolabs, catalog number: M0201L) 32 P-γ-ATP, 3,000 Ci/mmol, 3.3 µM (PerkinElmer, catalog number: BLU002A) Type XI low-melt agarose (Sigma-Aldrich, catalog number: A3038) Note: This product has been discontinued . .. Bts CI (New England Biolabs, catalog number: R0647L) β-Agarase I (New England Biolabs, catalog number: M0392L) 3 M sodium acetate (CH3 COONa), pH 5.2 (Sigma-Aldrich, catalog number: S2889) Isopropanol (Sigma-Aldrich, catalog number: 190764) Glycogen, molecular biology grade (Thermo Fisher Scientific, Thermo Scientific™, catalog number: R0561) Ethanol (Sigma-Aldrich, catalog number: E7023) 1 µM RFC (Replication Factor C; see for purification details) 5 µM PCNA (Proliferating Cellular Nuclear Antigen; see for purification details) 2 µM Pol ε (see for purification details) 2 µM Pol δ (see for purification details) 2 µM Pol α (see for purification details) 20 µM RPA (Replication Protein A; see for purification details) 32 P-α-dCTP, 3,000 Ci/mmol, 3.3 µM (PerkinElmer, catalog number: BLU013H) 32 P-α-dGTP, 3,000 Ci/mmol, 3.3 µM (PerkinElmer, catalog number: BLU514H) LE agarose (BioExpress, GeneMate, catalog number: E-3120-500) 10 N sodium hydroxide (NaOH) (Fisher Scientific, catalog number: SS255) Glycerol Xylene cylanol Tris-HCl, pH 8.0 Tris base (RPI, catalog number: T60040-500.0) Boric acid (RPI, catalog number: B32050-5000.0) Sodium citrate 1-Butanol Tris-acetate, pH 7.5 Bovine serum albumin (BSA) (New England Biolabs, catalog number: B9000S) Tris(2-carboxyethyl)phosphine (TCEP) pH 7.5 100 mM dithiothreitol (DTT) (Thermo Fisher Scientific, Thermo Scientific™, catalog number: R0861) Potassium glutamate Magnesium acetate 1% SDS 6× gel loading dye (see Recipes) TE buffer, pH 8.0 (see Recipes) 10× TBE (Tris/Borate/EDTA; see Recipes) DNA elution buffer (see Recipes) 20× SSC (see Recipes) 1-Butanol saturated water (see Recipes) 5× TDBG (see Recipes) 10× MK (see Recipes) dA/dC mix (see Recipes) dT/dG mix (see Recipes) T/G/C mix (see Recipes) Stop solution (see Recipes) Alkaline running buffer (see Recipes)

    Purification:

    Article Title: Restoration of Vision in the pde6?-deficient Dog, a Large Animal Model of Rod-cone Dystrophy
    Article Snippet: .. Pde6β PCR products were purified by Nucleospin Extract II (Macherey-Nagel, Hoerdt, France) before digestion by BtsCI (New England Biolabs, Ipswich, MA) for 2 hours at 50 °C according to the manufacturer's instructions and analyzed by 3.5% agarose gel electrophoresis. ..

    Article Title: Programming molecular topologies from single-stranded nucleic acids
    Article Snippet: .. The ssDNAs were cleaved off from the recombinant phage DNAs by using a BtsCI restriction enzyme (New England Biolabs), and were gel purified by using a 4% urea denaturing PAGE gel. ..

    Article Title: Folding complex DNA nanostructures from limited sets of reusable sequences
    Article Snippet: .. For the custom scaffold 24-helix bundle, the purified scaffold was subsequently digested with BtsCI (NEB, Catalog # R0647L) as follows: 10 μL of ssDNA at 100 nM with 2 μL of 10x CutSmart Buffer, 1 μL of BtsCI and 7 μL of ddH2 O was incubated at 50°C overnight. .. Note, that the final scaffold sequence will contain two dsDNA restriction sites for BtsCI (hairpin at each end, Supplementary Figure S2) and several ssDNA restriction sites for BtsCI.

    Article Title: Skin Characteristics in Patients with Pityriasis Versicolor Using Non-Invasive Method, MPA5
    Article Snippet: ABI) products were purified, using a LaboPass™ Gel (Cosmo, Seoul, Korea) and PCR Clean-up kit (Cosmo). .. Two restriction enzymes, Hha I (Koschem, Seoul, Korea) and BtsC I (NEB, London, England) were used to perform 26S rDNA-RFLP of Malassezia .

    Article Title: In vitro Assays for Eukaryotic Leading/Lagging Strand DNA Replication
    Article Snippet: .. Bts CI (New England Biolabs, catalog number: R0647L) β-Agarase I (New England Biolabs, catalog number: M0392L) 3 M sodium acetate (CH3 COONa), pH 5.2 (Sigma-Aldrich, catalog number: S2889) Isopropanol (Sigma-Aldrich, catalog number: 190764) Glycogen, molecular biology grade (Thermo Fisher Scientific, Thermo Scientific™, catalog number: R0561) Ethanol (Sigma-Aldrich, catalog number: E7023) 1 µM RFC (Replication Factor C; see for purification details) 5 µM PCNA (Proliferating Cellular Nuclear Antigen; see for purification details) 2 µM Pol ε (see for purification details) 2 µM Pol δ (see for purification details) 2 µM Pol α (see for purification details) 20 µM RPA (Replication Protein A; see for purification details) 32 P-α-dCTP, 3,000 Ci/mmol, 3.3 µM (PerkinElmer, catalog number: BLU013H) 32 P-α-dGTP, 3,000 Ci/mmol, 3.3 µM (PerkinElmer, catalog number: BLU514H) LE agarose (BioExpress, GeneMate, catalog number: E-3120-500) 10 N sodium hydroxide (NaOH) (Fisher Scientific, catalog number: SS255) Glycerol Xylene cylanol Tris-HCl, pH 8.0 Tris base (RPI, catalog number: T60040-500.0) Boric acid (RPI, catalog number: B32050-5000.0) Sodium citrate 1-Butanol Tris-acetate, pH 7.5 Bovine serum albumin (BSA) (New England Biolabs, catalog number: B9000S) Tris(2-carboxyethyl)phosphine (TCEP) pH 7.5 100 mM dithiothreitol (DTT) (Thermo Fisher Scientific, Thermo Scientific™, catalog number: R0861) Potassium glutamate Magnesium acetate 1% SDS 6× gel loading dye (see Recipes) TE buffer, pH 8.0 (see Recipes) 10× TBE (Tris/Borate/EDTA; see Recipes) DNA elution buffer (see Recipes) 20× SSC (see Recipes) 1-Butanol saturated water (see Recipes) 5× TDBG (see Recipes) 10× MK (see Recipes) dA/dC mix (see Recipes) dT/dG mix (see Recipes) T/G/C mix (see Recipes) Stop solution (see Recipes) Alkaline running buffer (see Recipes) .. Heating block ( e.g., VWR, catalog number: 12621-084) Fraction collector ( e.g., Gilson, model: F203B) Variable mode gel imager ( e.g., GE Typhoon) UV-vis spectrophotometer ( e.g., Thermo Fisher Scientific, Thermo Scientific™, model: NanoDrop™ 2000) Vacuum dessicator ( e.g., Thermo Fisher Scientific, Thermo Scientific™, catalog number: 5309-0250) UV light box UV blocking face shield (e.g., Sigma-Aldrich, catalog number: F8142) Note: This product has been discontinued.

    Polymerase Chain Reaction:

    Article Title: Quinolone resistance mechanisms among third-generation cephalosporin resistant isolates of Enterobacter spp. in a Bulgarian university hospital
    Article Snippet: Quinolone resistance determining regions (QRDR) and PMQR detection QRDR in gyrA and parC were amplified by PCR and sequenced as described previously. .. The exact allele of aac(6ʹ)-Ib enzymes was determined with restriction with BtsCI (New England Biolabs, UK).

    Article Title: Restoration of Vision in the pde6?-deficient Dog, a Large Animal Model of Rod-cone Dystrophy
    Article Snippet: .. Pde6β PCR products were purified by Nucleospin Extract II (Macherey-Nagel, Hoerdt, France) before digestion by BtsCI (New England Biolabs, Ipswich, MA) for 2 hours at 50 °C according to the manufacturer's instructions and analyzed by 3.5% agarose gel electrophoresis. ..

    Article Title: SIAE Rare Variants in Juvenile Idiopathic Arthritis and Primary Antibody Deficiencies
    Article Snippet: .. In particular, for the detection of p.Q343P mutation, a 360 bp fragment (encompassing exon 8) was amplified by PCR and subjected to BtsCI overnight digestion (New England Biolabs, Ipswich, UK) at 50°C. ..

    Article Title: Lack of effect of apolipoprotein C3 polymorphisms on indices of liver steatosis, lipid profile and insulin resistance in obese Southern Europeans
    Article Snippet: A 497-bp fragment was amplified and enzymatically restricted using BtsCI (New England Biolabs, Ipswich, MA, USA). .. The resulted enzyme-digested fragments of the PCR products were fractionated on 3.5% agarose gels, stained with ethidium bromide, and visualized with an imaging system (Amersham-Pharmacia Biosciences).

    Article Title: Skin Characteristics in Patients with Pityriasis Versicolor Using Non-Invasive Method, MPA5
    Article Snippet: ABI) products were purified, using a LaboPass™ Gel (Cosmo, Seoul, Korea) and PCR Clean-up kit (Cosmo). .. Two restriction enzymes, Hha I (Koschem, Seoul, Korea) and BtsC I (NEB, London, England) were used to perform 26S rDNA-RFLP of Malassezia .

    Article Title: Analysis of quinolone-resistance in commensal and diarrheagenic Escherichia coli isolates from infants in Lima, Peru
    Article Snippet: .. Positive PCR products were digested with BtsC I (New England Biolabs, Beijing, China) to identify the aac-(6′)-Ib-cr variant. .. The qnrA, qnrB, qnrS, qepA and oqxAB genes were also detected by conventional PCR, using the primers listed in Table .

    Article Title: Chromosomal and plasmid-mediated fluoroquinolone resistance mechanisms among broad-spectrum-cephalosporin-resistant Escherichia coli isolates recovered from companion animals in the USA
    Article Snippet: All E. coli isolates that tested positive for aac(6 ′ )-Ib-cr were confirmed by enzymatic digestion with BtsCI restriction endonuclease (New England Biolabs, Ipswich, MA, USA) or by DNA sequencing. .. The PCR products for qepA and qnrS genes were sequenced.

    Article Title: Mice expressing a human KATP channel mutation have altered channel ATP sensitivity but no cardiac abnormalities
    Article Snippet: .. PCR products were digested with BtsCI (New England Biolabs, Hitchin, UK) for ∼2 h at 50°C. .. Digested PCR fragments were visualised on an agarose gel with ethidium bromide.

    Article Title: Expression of an activating mutation in the gene encoding the KATP channel subunit Kir6.2 in mouse pancreatic ? cells recapitulates neonatal diabetes
    Article Snippet: .. After 40 cycles (95°C for 30 seconds; 60°C for 30 seconds; 72°C for 1 minute), PCR products were digested with BtsCI restriction enzyme (New England BioLabs) for approximately 1 hour at 50°C and digested PCR fragments visualized on a 2% agarose gel. .. Sequences of primers used for GFP amplification were as follows: forward, 5′-GAGGTGAAGTTCGAGGGCGAC-3′; and reverse, 5′-CAGGACCATGTGATCGCGCTT-3′.

    Polyacrylamide Gel Electrophoresis:

    Article Title: Programming molecular topologies from single-stranded nucleic acids
    Article Snippet: .. The ssDNAs were cleaved off from the recombinant phage DNAs by using a BtsCI restriction enzyme (New England Biolabs), and were gel purified by using a 4% urea denaturing PAGE gel. ..

    Polymorphism Assay:

    Article Title: SIAE Rare Variants in Juvenile Idiopathic Arthritis and Primary Antibody Deficiencies
    Article Snippet: Thus, for the detection of c.1320+33T > C and p.Q343P alterations, we set up PCR restriction fragment length polymorphism assay (RFLP) protocols. .. In particular, for the detection of p.Q343P mutation, a 360 bp fragment (encompassing exon 8) was amplified by PCR and subjected to BtsCI overnight digestion (New England Biolabs, Ipswich, UK) at 50°C.

    Mouse Assay:

    Article Title: Mice expressing a human KATP channel mutation have altered channel ATP sensitivity but no cardiac abnormalities
    Article Snippet: Semi-quantitative PCR Mouse Kcnj11 transcript was amplified by PCR using cDNA prepared from muscle and brain tissues from m-V59M and control mice, as previously described [ ]. .. PCR products were digested with BtsCI (New England Biolabs, Hitchin, UK) for ∼2 h at 50°C.

    Article Title: Expression of an activating mutation in the gene encoding the KATP channel subunit Kir6.2 in mouse pancreatic ? cells recapitulates neonatal diabetes
    Article Snippet: Mouse Kir6.2 transcript was amplified by PCR using cDNA prepared from islets isolated from either V59M or WT 5-week-old mice. .. After 40 cycles (95°C for 30 seconds; 60°C for 30 seconds; 72°C for 1 minute), PCR products were digested with BtsCI restriction enzyme (New England BioLabs) for approximately 1 hour at 50°C and digested PCR fragments visualized on a 2% agarose gel.

    Plasmid Preparation:

    Article Title: Quinolone resistance mechanisms among third-generation cephalosporin resistant isolates of Enterobacter spp. in a Bulgarian university hospital
    Article Snippet: Plasmid mediated quinolone resistant determinants qnrA, qnrB, qnrC, qnr D, qnrS, and qepA were amplified and sequenced with ABI 3130xl Genetic Analyzer. .. The exact allele of aac(6ʹ)-Ib enzymes was determined with restriction with BtsCI (New England Biolabs, UK).

    Article Title: Programming molecular topologies from single-stranded nucleic acids
    Article Snippet: Topological control experiments The Biobasic company (Biobasic.com) synthesized four ssDNAs with the customized sequences and then cloned these sequences into a pBluescript SK( + ) vector (Biobasic), with the gene sequences flanked by two BtsCI restriction sites. .. The ssDNAs were cleaved off from the recombinant phage DNAs by using a BtsCI restriction enzyme (New England Biolabs), and were gel purified by using a 4% urea denaturing PAGE gel.

    Software:

    Article Title: Quinolone resistance mechanisms among third-generation cephalosporin resistant isolates of Enterobacter spp. in a Bulgarian university hospital
    Article Snippet: The exact allele of aac(6ʹ)-Ib enzymes was determined with restriction with BtsCI (New England Biolabs, UK). .. The nucleotide sequences were analyzed with Chromas Lite 2.01 (Technelysium Pty Ltd) DNAMAN version 8.0 Software (Lynnon BioSoft) and NCBI Blast tool (http://www.ncbi.nlm.nih.gov).

    Electrophoresis:

    Article Title: Skin Characteristics in Patients with Pityriasis Versicolor Using Non-Invasive Method, MPA5
    Article Snippet: Two restriction enzymes, Hha I (Koschem, Seoul, Korea) and BtsC I (NEB, London, England) were used to perform 26S rDNA-RFLP of Malassezia . .. After the reaction at 37℃ for 3 hours, the electrophoresis was done with 3% (w/v) Seakem LE agarose gel (Takara Biomedicals, Otsu, Japan) by 100 volt and stained with ethidium bromide.

    Genotyping Assay:

    Article Title: Lack of effect of apolipoprotein C3 polymorphisms on indices of liver steatosis, lipid profile and insulin resistance in obese Southern Europeans
    Article Snippet: Paragraph title: Genotyping assay ... A 497-bp fragment was amplified and enzymatically restricted using BtsCI (New England Biolabs, Ipswich, MA, USA).

    Agarose Gel Electrophoresis:

    Article Title: Restoration of Vision in the pde6?-deficient Dog, a Large Animal Model of Rod-cone Dystrophy
    Article Snippet: .. Pde6β PCR products were purified by Nucleospin Extract II (Macherey-Nagel, Hoerdt, France) before digestion by BtsCI (New England Biolabs, Ipswich, MA) for 2 hours at 50 °C according to the manufacturer's instructions and analyzed by 3.5% agarose gel electrophoresis. ..

    Article Title: Skin Characteristics in Patients with Pityriasis Versicolor Using Non-Invasive Method, MPA5
    Article Snippet: Two restriction enzymes, Hha I (Koschem, Seoul, Korea) and BtsC I (NEB, London, England) were used to perform 26S rDNA-RFLP of Malassezia . .. After the reaction at 37℃ for 3 hours, the electrophoresis was done with 3% (w/v) Seakem LE agarose gel (Takara Biomedicals, Otsu, Japan) by 100 volt and stained with ethidium bromide.

    Article Title: Mice expressing a human KATP channel mutation have altered channel ATP sensitivity but no cardiac abnormalities
    Article Snippet: PCR products were digested with BtsCI (New England Biolabs, Hitchin, UK) for ∼2 h at 50°C. .. Digested PCR fragments were visualised on an agarose gel with ethidium bromide.

    Article Title: Expression of an activating mutation in the gene encoding the KATP channel subunit Kir6.2 in mouse pancreatic ? cells recapitulates neonatal diabetes
    Article Snippet: .. After 40 cycles (95°C for 30 seconds; 60°C for 30 seconds; 72°C for 1 minute), PCR products were digested with BtsCI restriction enzyme (New England BioLabs) for approximately 1 hour at 50°C and digested PCR fragments visualized on a 2% agarose gel. .. Sequences of primers used for GFP amplification were as follows: forward, 5′-GAGGTGAAGTTCGAGGGCGAC-3′; and reverse, 5′-CAGGACCATGTGATCGCGCTT-3′.

    Concentration Assay:

    Article Title: Programming molecular topologies from single-stranded nucleic acids
    Article Snippet: The ssDNAs were cleaved off from the recombinant phage DNAs by using a BtsCI restriction enzyme (New England Biolabs), and were gel purified by using a 4% urea denaturing PAGE gel. .. The two linear dsDNAs were mixed in a 20 nM concentration in a 1× TAE-Mg buffer, annealed from 65 °C to 25 °C at 1 °C per 20 min to form the desired paranemic cohesion interactions, and were then characterized by AFM imaging.

    Article Title: Folding complex DNA nanostructures from limited sets of reusable sequences
    Article Snippet: Finally, the pellet was air dried and resuspended in TE buffer (5 mM Tris pH 8.5 and 1 mM EDTA) - the volume will depend on desired final concentration of scaffold. .. For the custom scaffold 24-helix bundle, the purified scaffold was subsequently digested with BtsCI (NEB, Catalog # R0647L) as follows: 10 μL of ssDNA at 100 nM with 2 μL of 10x CutSmart Buffer, 1 μL of BtsCI and 7 μL of ddH2 O was incubated at 50°C overnight.

    Marker:

    Article Title: In vitro Assays for Eukaryotic Leading/Lagging Strand DNA Replication
    Article Snippet: T4 ligase, including 10× ligase buffer (New England Biolabs, catalog number: M0202M) 100 mM ATP (GE Healthcare, catalog number: 27-2056-01) 0.5 M EDTA, disodium salt (Sigma-Aldrich, catalog number: E5134) 5 M NaCl (Sigma-Aldrich, catalog number: S9888) Sepharose 4B size exclusion chromatography resin (GE Healthcare, catalog number: 17012001) 1 kb MW marker (New England Biolabs, catalog number: N3232L) Ethidium bromide (EthBr, 10 mg/ml) (Thermo Fisher Scientific, Invitrogen™, catalog number: 15585011) T4 kinase and 10× T4 kinase buffer (New England Biolabs, catalog number: M0201L) 32 P-γ-ATP, 3,000 Ci/mmol, 3.3 µM (PerkinElmer, catalog number: BLU002A) Type XI low-melt agarose (Sigma-Aldrich, catalog number: A3038) Note: This product has been discontinued . .. Bts CI (New England Biolabs, catalog number: R0647L) β-Agarase I (New England Biolabs, catalog number: M0392L) 3 M sodium acetate (CH3 COONa), pH 5.2 (Sigma-Aldrich, catalog number: S2889) Isopropanol (Sigma-Aldrich, catalog number: 190764) Glycogen, molecular biology grade (Thermo Fisher Scientific, Thermo Scientific™, catalog number: R0561) Ethanol (Sigma-Aldrich, catalog number: E7023) 1 µM RFC (Replication Factor C; see for purification details) 5 µM PCNA (Proliferating Cellular Nuclear Antigen; see for purification details) 2 µM Pol ε (see for purification details) 2 µM Pol δ (see for purification details) 2 µM Pol α (see for purification details) 20 µM RPA (Replication Protein A; see for purification details) 32 P-α-dCTP, 3,000 Ci/mmol, 3.3 µM (PerkinElmer, catalog number: BLU013H) 32 P-α-dGTP, 3,000 Ci/mmol, 3.3 µM (PerkinElmer, catalog number: BLU514H) LE agarose (BioExpress, GeneMate, catalog number: E-3120-500) 10 N sodium hydroxide (NaOH) (Fisher Scientific, catalog number: SS255) Glycerol Xylene cylanol Tris-HCl, pH 8.0 Tris base (RPI, catalog number: T60040-500.0) Boric acid (RPI, catalog number: B32050-5000.0) Sodium citrate 1-Butanol Tris-acetate, pH 7.5 Bovine serum albumin (BSA) (New England Biolabs, catalog number: B9000S) Tris(2-carboxyethyl)phosphine (TCEP) pH 7.5 100 mM dithiothreitol (DTT) (Thermo Fisher Scientific, Thermo Scientific™, catalog number: R0861) Potassium glutamate Magnesium acetate 1% SDS 6× gel loading dye (see Recipes) TE buffer, pH 8.0 (see Recipes) 10× TBE (Tris/Borate/EDTA; see Recipes) DNA elution buffer (see Recipes) 20× SSC (see Recipes) 1-Butanol saturated water (see Recipes) 5× TDBG (see Recipes) 10× MK (see Recipes) dA/dC mix (see Recipes) dT/dG mix (see Recipes) T/G/C mix (see Recipes) Stop solution (see Recipes) Alkaline running buffer (see Recipes)

    Staining:

    Article Title: Lack of effect of apolipoprotein C3 polymorphisms on indices of liver steatosis, lipid profile and insulin resistance in obese Southern Europeans
    Article Snippet: A 497-bp fragment was amplified and enzymatically restricted using BtsCI (New England Biolabs, Ipswich, MA, USA). .. The resulted enzyme-digested fragments of the PCR products were fractionated on 3.5% agarose gels, stained with ethidium bromide, and visualized with an imaging system (Amersham-Pharmacia Biosciences).

    Article Title: Skin Characteristics in Patients with Pityriasis Versicolor Using Non-Invasive Method, MPA5
    Article Snippet: Two restriction enzymes, Hha I (Koschem, Seoul, Korea) and BtsC I (NEB, London, England) were used to perform 26S rDNA-RFLP of Malassezia . .. After the reaction at 37℃ for 3 hours, the electrophoresis was done with 3% (w/v) Seakem LE agarose gel (Takara Biomedicals, Otsu, Japan) by 100 volt and stained with ethidium bromide.

    Variant Assay:

    Article Title: SIAE Rare Variants in Juvenile Idiopathic Arthritis and Primary Antibody Deficiencies
    Article Snippet: In particular, for the detection of p.Q343P mutation, a 360 bp fragment (encompassing exon 8) was amplified by PCR and subjected to BtsCI overnight digestion (New England Biolabs, Ipswich, UK) at 50°C. .. For the detection of c.1320+33T > C genetic variant, a 433 bp fragment (encompassing exon 9) was amplified by PCR and subjected to digestion with TspDTI (EURx , Gdańsk, Poland) at 70°C for 9 hours.

    Article Title: Lack of effect of apolipoprotein C3 polymorphisms on indices of liver steatosis, lipid profile and insulin resistance in obese Southern Europeans
    Article Snippet: The APOC3 rs2854116 gene variant was assayed by the restriction fragment length polymorphism (RFLP) technique. .. A 497-bp fragment was amplified and enzymatically restricted using BtsCI (New England Biolabs, Ipswich, MA, USA).

    Article Title: Analysis of quinolone-resistance in commensal and diarrheagenic Escherichia coli isolates from infants in Lima, Peru
    Article Snippet: .. Positive PCR products were digested with BtsC I (New England Biolabs, Beijing, China) to identify the aac-(6′)-Ib-cr variant. .. The qnrA, qnrB, qnrS, qepA and oqxAB genes were also detected by conventional PCR, using the primers listed in Table .

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    New England Biolabs btsci restriction enzyme
    Btsci Restriction Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/btsci restriction enzyme/product/New England Biolabs
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    btsci restriction enzyme - by Bioz Stars, 2020-03
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