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    Name:
    BtsCI
    Description:
    BtsCI 2 000 units
    Catalog Number:
    R0647L
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    Category:
    Restriction Enzymes
    Size:
    2 000 units
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    New England Biolabs btsci
    BtsCI
    BtsCI 2 000 units
    https://www.bioz.com/result/btsci/product/New England Biolabs
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    btsci - by Bioz Stars, 2021-06
    96/100 stars

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    1) Product Images from "Mice expressing a human KATP channel mutation have altered channel ATP sensitivity but no cardiac abnormalities"

    Article Title: Mice expressing a human KATP channel mutation have altered channel ATP sensitivity but no cardiac abnormalities

    Journal: Diabetologia

    doi: 10.1007/s00125-011-2428-6

    Kcnj11 expression in the heart. a Kcnj11 expression in tissue isolated from ROSA, WT, Mck-Cre and m-V59M mice. WT, but not mutant (V59M), Kcnj11 cDNA is cut by the restriction enzyme BtsCI: the presence of two bands thus indicates the presence of the WT gene only and three bands indicates both WT and mutant genes. Data are representative of experiments on seven ROSA, four m-V59M, four WT and four Mck-Cre mice. sk1, quadriceps muscle; sk2, triceps muscle; sk3, diaphragm. b,c Quantitative PCR showing expression of Kcnj11 in heart and brain ( b ) and Gfp in heart ( c ) of control (grey bars, n = 5) and m-V59M mice (white bars, n = 5), relative to a panel of housekeeping genes. * p
    Figure Legend Snippet: Kcnj11 expression in the heart. a Kcnj11 expression in tissue isolated from ROSA, WT, Mck-Cre and m-V59M mice. WT, but not mutant (V59M), Kcnj11 cDNA is cut by the restriction enzyme BtsCI: the presence of two bands thus indicates the presence of the WT gene only and three bands indicates both WT and mutant genes. Data are representative of experiments on seven ROSA, four m-V59M, four WT and four Mck-Cre mice. sk1, quadriceps muscle; sk2, triceps muscle; sk3, diaphragm. b,c Quantitative PCR showing expression of Kcnj11 in heart and brain ( b ) and Gfp in heart ( c ) of control (grey bars, n = 5) and m-V59M mice (white bars, n = 5), relative to a panel of housekeeping genes. * p

    Techniques Used: Expressing, Isolation, Mouse Assay, Mutagenesis, Real-time Polymerase Chain Reaction

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Restoration of Vision in the pde6?-deficient Dog, a Large Animal Model of Rod-cone Dystrophy
    Article Snippet: Actin PCR products were resolved on a 1.5% agarose gel electrophoresis. .. Pde6β PCR products were purified by Nucleospin Extract II (Macherey-Nagel, Hoerdt, France) before digestion by BtsCI (New England Biolabs, Ipswich, MA) for 2 hours at 50 °C according to the manufacturer's instructions and analyzed by 3.5% agarose gel electrophoresis. ..

    Article Title: Extreme allelic heterogeneity at a Caenorhabditis elegans beta-tubulin locus explains natural resistance to benzimidazoles
    Article Snippet: .. PCRs were performed using the primers oECA1297 and oECA1298, and PCR products were incubated with BTsCI restriction enzyme (R0647S, New England Biolabs, Ipswich, MA) to identify successfully edited animals by differential band patterns. .. For genotyping of ben-1 deletion strains, the primers oECA1301 and oECA1302 were used and successful deletions were identified by shorter PCR products.

    Article Title: Mice expressing a human KATP channel mutation have altered channel ATP sensitivity but no cardiac abnormalities
    Article Snippet: Semi-quantitative PCR Mouse Kcnj11 transcript was amplified by PCR using cDNA prepared from muscle and brain tissues from m-V59M and control mice, as previously described [ ]. .. PCR products were digested with BtsCI (New England Biolabs, Hitchin, UK) for ∼2 h at 50°C. .. Digested PCR fragments were visualised on an agarose gel with ethidium bromide.

    Purification:

    Article Title: Restoration of Vision in the pde6?-deficient Dog, a Large Animal Model of Rod-cone Dystrophy
    Article Snippet: Actin PCR products were resolved on a 1.5% agarose gel electrophoresis. .. Pde6β PCR products were purified by Nucleospin Extract II (Macherey-Nagel, Hoerdt, France) before digestion by BtsCI (New England Biolabs, Ipswich, MA) for 2 hours at 50 °C according to the manufacturer's instructions and analyzed by 3.5% agarose gel electrophoresis. ..

    Article Title: In vitro Assays for Eukaryotic Leading/Lagging Strand DNA Replication
    Article Snippet: T4 ligase, including 10× ligase buffer (New England Biolabs, catalog number: M0202M) 100 mM ATP (GE Healthcare, catalog number: 27-2056-01) 0.5 M EDTA, disodium salt (Sigma-Aldrich, catalog number: E5134) 5 M NaCl (Sigma-Aldrich, catalog number: S9888) Sepharose 4B size exclusion chromatography resin (GE Healthcare, catalog number: 17012001) 1 kb MW marker (New England Biolabs, catalog number: N3232L) Ethidium bromide (EthBr, 10 mg/ml) (Thermo Fisher Scientific, Invitrogen™, catalog number: 15585011) T4 kinase and 10× T4 kinase buffer (New England Biolabs, catalog number: M0201L) 32 P-γ-ATP, 3,000 Ci/mmol, 3.3 µM (PerkinElmer, catalog number: BLU002A) Type XI low-melt agarose (Sigma-Aldrich, catalog number: A3038) Note: This product has been discontinued . .. Bts CI (New England Biolabs, catalog number: R0647L) β-Agarase I (New England Biolabs, catalog number: M0392L) 3 M sodium acetate (CH3 COONa), pH 5.2 (Sigma-Aldrich, catalog number: S2889) Isopropanol (Sigma-Aldrich, catalog number: 190764) Glycogen, molecular biology grade (Thermo Fisher Scientific, Thermo Scientific™, catalog number: R0561) Ethanol (Sigma-Aldrich, catalog number: E7023) 1 µM RFC (Replication Factor C; see for purification details) 5 µM PCNA (Proliferating Cellular Nuclear Antigen; see for purification details) 2 µM Pol ε (see for purification details) 2 µM Pol δ (see for purification details) 2 µM Pol α (see for purification details) 20 µM RPA (Replication Protein A; see for purification details) 32 P-α-dCTP, 3,000 Ci/mmol, 3.3 µM (PerkinElmer, catalog number: BLU013H) 32 P-α-dGTP, 3,000 Ci/mmol, 3.3 µM (PerkinElmer, catalog number: BLU514H) LE agarose (BioExpress, GeneMate, catalog number: E-3120-500) 10 N sodium hydroxide (NaOH) (Fisher Scientific, catalog number: SS255) Glycerol Xylene cylanol Tris-HCl, pH 8.0 Tris base (RPI, catalog number: T60040-500.0) Boric acid (RPI, catalog number: B32050-5000.0) Sodium citrate 1-Butanol Tris-acetate, pH 7.5 Bovine serum albumin (BSA) (New England Biolabs, catalog number: B9000S) Tris(2-carboxyethyl)phosphine (TCEP) pH 7.5 100 mM dithiothreitol (DTT) (Thermo Fisher Scientific, Thermo Scientific™, catalog number: R0861) Potassium glutamate Magnesium acetate 1% SDS 6× gel loading dye (see Recipes) TE buffer, pH 8.0 (see Recipes) 10× TBE (Tris/Borate/EDTA; see Recipes) DNA elution buffer (see Recipes) 20× SSC (see Recipes) 1-Butanol saturated water (see Recipes) 5× TDBG (see Recipes) 10× MK (see Recipes) dA/dC mix (see Recipes) dT/dG mix (see Recipes) T/G/C mix (see Recipes) Stop solution (see Recipes) Alkaline running buffer (see Recipes) .. Heating block ( e.g., VWR, catalog number: 12621-084) Fraction collector ( e.g., Gilson, model: F203B) Variable mode gel imager ( e.g., GE Typhoon) UV-vis spectrophotometer ( e.g., Thermo Fisher Scientific, Thermo Scientific™, model: NanoDrop™ 2000) Vacuum dessicator ( e.g., Thermo Fisher Scientific, Thermo Scientific™, catalog number: 5309-0250) UV light box UV blocking face shield (e.g., Sigma-Aldrich, catalog number: F8142) Note: This product has been discontinued.

    Agarose Gel Electrophoresis:

    Article Title: Restoration of Vision in the pde6?-deficient Dog, a Large Animal Model of Rod-cone Dystrophy
    Article Snippet: Actin PCR products were resolved on a 1.5% agarose gel electrophoresis. .. Pde6β PCR products were purified by Nucleospin Extract II (Macherey-Nagel, Hoerdt, France) before digestion by BtsCI (New England Biolabs, Ipswich, MA) for 2 hours at 50 °C according to the manufacturer's instructions and analyzed by 3.5% agarose gel electrophoresis. ..

    Incubation:

    Article Title: Extreme allelic heterogeneity at a Caenorhabditis elegans beta-tubulin locus explains natural resistance to benzimidazoles
    Article Snippet: .. PCRs were performed using the primers oECA1297 and oECA1298, and PCR products were incubated with BTsCI restriction enzyme (R0647S, New England Biolabs, Ipswich, MA) to identify successfully edited animals by differential band patterns. .. For genotyping of ben-1 deletion strains, the primers oECA1301 and oECA1302 were used and successful deletions were identified by shorter PCR products.

    Recombinase Polymerase Amplification:

    Article Title: In vitro Assays for Eukaryotic Leading/Lagging Strand DNA Replication
    Article Snippet: T4 ligase, including 10× ligase buffer (New England Biolabs, catalog number: M0202M) 100 mM ATP (GE Healthcare, catalog number: 27-2056-01) 0.5 M EDTA, disodium salt (Sigma-Aldrich, catalog number: E5134) 5 M NaCl (Sigma-Aldrich, catalog number: S9888) Sepharose 4B size exclusion chromatography resin (GE Healthcare, catalog number: 17012001) 1 kb MW marker (New England Biolabs, catalog number: N3232L) Ethidium bromide (EthBr, 10 mg/ml) (Thermo Fisher Scientific, Invitrogen™, catalog number: 15585011) T4 kinase and 10× T4 kinase buffer (New England Biolabs, catalog number: M0201L) 32 P-γ-ATP, 3,000 Ci/mmol, 3.3 µM (PerkinElmer, catalog number: BLU002A) Type XI low-melt agarose (Sigma-Aldrich, catalog number: A3038) Note: This product has been discontinued . .. Bts CI (New England Biolabs, catalog number: R0647L) β-Agarase I (New England Biolabs, catalog number: M0392L) 3 M sodium acetate (CH3 COONa), pH 5.2 (Sigma-Aldrich, catalog number: S2889) Isopropanol (Sigma-Aldrich, catalog number: 190764) Glycogen, molecular biology grade (Thermo Fisher Scientific, Thermo Scientific™, catalog number: R0561) Ethanol (Sigma-Aldrich, catalog number: E7023) 1 µM RFC (Replication Factor C; see for purification details) 5 µM PCNA (Proliferating Cellular Nuclear Antigen; see for purification details) 2 µM Pol ε (see for purification details) 2 µM Pol δ (see for purification details) 2 µM Pol α (see for purification details) 20 µM RPA (Replication Protein A; see for purification details) 32 P-α-dCTP, 3,000 Ci/mmol, 3.3 µM (PerkinElmer, catalog number: BLU013H) 32 P-α-dGTP, 3,000 Ci/mmol, 3.3 µM (PerkinElmer, catalog number: BLU514H) LE agarose (BioExpress, GeneMate, catalog number: E-3120-500) 10 N sodium hydroxide (NaOH) (Fisher Scientific, catalog number: SS255) Glycerol Xylene cylanol Tris-HCl, pH 8.0 Tris base (RPI, catalog number: T60040-500.0) Boric acid (RPI, catalog number: B32050-5000.0) Sodium citrate 1-Butanol Tris-acetate, pH 7.5 Bovine serum albumin (BSA) (New England Biolabs, catalog number: B9000S) Tris(2-carboxyethyl)phosphine (TCEP) pH 7.5 100 mM dithiothreitol (DTT) (Thermo Fisher Scientific, Thermo Scientific™, catalog number: R0861) Potassium glutamate Magnesium acetate 1% SDS 6× gel loading dye (see Recipes) TE buffer, pH 8.0 (see Recipes) 10× TBE (Tris/Borate/EDTA; see Recipes) DNA elution buffer (see Recipes) 20× SSC (see Recipes) 1-Butanol saturated water (see Recipes) 5× TDBG (see Recipes) 10× MK (see Recipes) dA/dC mix (see Recipes) dT/dG mix (see Recipes) T/G/C mix (see Recipes) Stop solution (see Recipes) Alkaline running buffer (see Recipes) .. Heating block ( e.g., VWR, catalog number: 12621-084) Fraction collector ( e.g., Gilson, model: F203B) Variable mode gel imager ( e.g., GE Typhoon) UV-vis spectrophotometer ( e.g., Thermo Fisher Scientific, Thermo Scientific™, model: NanoDrop™ 2000) Vacuum dessicator ( e.g., Thermo Fisher Scientific, Thermo Scientific™, catalog number: 5309-0250) UV light box UV blocking face shield (e.g., Sigma-Aldrich, catalog number: F8142) Note: This product has been discontinued.

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  • 96
    New England Biolabs btsci
    Kcnj11 expression in the heart. a Kcnj11 expression in tissue isolated from ROSA, WT, Mck-Cre and m-V59M mice. WT, but not mutant (V59M), Kcnj11 cDNA is cut by the restriction enzyme <t>BtsCI:</t> the presence of two bands thus indicates the presence of the WT gene only and three bands indicates both WT and mutant genes. Data are representative of experiments on seven ROSA, four m-V59M, four WT and four Mck-Cre mice. sk1, quadriceps muscle; sk2, triceps muscle; sk3, diaphragm. b,c Quantitative <t>PCR</t> showing expression of Kcnj11 in heart and brain ( b ) and Gfp in heart ( c ) of control (grey bars, n = 5) and m-V59M mice (white bars, n = 5), relative to a panel of housekeeping genes. * p
    Btsci, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/btsci/product/New England Biolabs
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
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    Kcnj11 expression in the heart. a Kcnj11 expression in tissue isolated from ROSA, WT, Mck-Cre and m-V59M mice. WT, but not mutant (V59M), Kcnj11 cDNA is cut by the restriction enzyme BtsCI: the presence of two bands thus indicates the presence of the WT gene only and three bands indicates both WT and mutant genes. Data are representative of experiments on seven ROSA, four m-V59M, four WT and four Mck-Cre mice. sk1, quadriceps muscle; sk2, triceps muscle; sk3, diaphragm. b,c Quantitative PCR showing expression of Kcnj11 in heart and brain ( b ) and Gfp in heart ( c ) of control (grey bars, n = 5) and m-V59M mice (white bars, n = 5), relative to a panel of housekeeping genes. * p

    Journal: Diabetologia

    Article Title: Mice expressing a human KATP channel mutation have altered channel ATP sensitivity but no cardiac abnormalities

    doi: 10.1007/s00125-011-2428-6

    Figure Lengend Snippet: Kcnj11 expression in the heart. a Kcnj11 expression in tissue isolated from ROSA, WT, Mck-Cre and m-V59M mice. WT, but not mutant (V59M), Kcnj11 cDNA is cut by the restriction enzyme BtsCI: the presence of two bands thus indicates the presence of the WT gene only and three bands indicates both WT and mutant genes. Data are representative of experiments on seven ROSA, four m-V59M, four WT and four Mck-Cre mice. sk1, quadriceps muscle; sk2, triceps muscle; sk3, diaphragm. b,c Quantitative PCR showing expression of Kcnj11 in heart and brain ( b ) and Gfp in heart ( c ) of control (grey bars, n = 5) and m-V59M mice (white bars, n = 5), relative to a panel of housekeeping genes. * p

    Article Snippet: PCR products were digested with BtsCI (New England Biolabs, Hitchin, UK) for ∼2 h at 50°C.

    Techniques: Expressing, Isolation, Mouse Assay, Mutagenesis, Real-time Polymerase Chain Reaction

    PCR-RFLP patterns of 26S rDNA PCR digested with restriction enzymes (A) Hha I, (B) BtsC I of 11 Malassezia standard strains in hyperpigmented lesions of 21 patients. Lanes: M: molecular Marker, 1: Malassezia globosa (CBS7966), 2: M. globosa (CBS7966), 3: M. restricta (KCTC7848), 4: M. globosa (CBS7966), 5: M. restricta (KCTC7848), 6: M. restricta (KCTC7848), 7: M. restricta (KCTC7848), 8: M. slooffiae (KCTC17431), 9: M. globosa (CBS7966), 10: M. globosa (CBS7966), 11: M. restricta (KCTC7848), 12: M. globosa (CBS7966), 13: M. globosa (CBS7966), 14: M. furfur (KCTC7743), 15: M. slooffiae (KCTC17431), 16: M. globosa (CBS7966), 17: M. globosa (CBS7966), 18: M. restricta (KCTC7848), 19: M. restricta (KCTC7848), 20: M. furfur (KCTC7743), 21: M. sympodialis (KCTC7985). PCR: polymerase chain reaction, RFLP: restriction fragment length polymorphism.

    Journal: Annals of Dermatology

    Article Title: Skin Characteristics in Patients with Pityriasis Versicolor Using Non-Invasive Method, MPA5

    doi: 10.5021/ad.2012.24.4.444

    Figure Lengend Snippet: PCR-RFLP patterns of 26S rDNA PCR digested with restriction enzymes (A) Hha I, (B) BtsC I of 11 Malassezia standard strains in hyperpigmented lesions of 21 patients. Lanes: M: molecular Marker, 1: Malassezia globosa (CBS7966), 2: M. globosa (CBS7966), 3: M. restricta (KCTC7848), 4: M. globosa (CBS7966), 5: M. restricta (KCTC7848), 6: M. restricta (KCTC7848), 7: M. restricta (KCTC7848), 8: M. slooffiae (KCTC17431), 9: M. globosa (CBS7966), 10: M. globosa (CBS7966), 11: M. restricta (KCTC7848), 12: M. globosa (CBS7966), 13: M. globosa (CBS7966), 14: M. furfur (KCTC7743), 15: M. slooffiae (KCTC17431), 16: M. globosa (CBS7966), 17: M. globosa (CBS7966), 18: M. restricta (KCTC7848), 19: M. restricta (KCTC7848), 20: M. furfur (KCTC7743), 21: M. sympodialis (KCTC7985). PCR: polymerase chain reaction, RFLP: restriction fragment length polymorphism.

    Article Snippet: Two restriction enzymes, Hha I (Koschem, Seoul, Korea) and BtsC I (NEB, London, England) were used to perform 26S rDNA-RFLP of Malassezia .

    Techniques: Polymerase Chain Reaction, Marker

    PCR-RFLP patterns of 26S rDNA PCR digested with restriction enzymes (A) Hha I, (B) BtsC I of 11 Malassezia standard strains in hypopigmented lesions of 9 patients. Lanes: M: molecular Marker , 1: Malassezia slooffiae (KCTC17431), 2: M. globosa (CBS7966), 3: M. restricta (KCTC7848), 4: M. restricta (KCTC7848), 5: M. globosa (CBS7966), 6: M. globosa (CBS7966), 7: M. globosa (CBS7966), 8: M. sympodialis (KCTC7985), 9: M. furfur (KCTC7743). PCR: polymerase chain reaction, RFLP: restriction fragment length polymorphism.

    Journal: Annals of Dermatology

    Article Title: Skin Characteristics in Patients with Pityriasis Versicolor Using Non-Invasive Method, MPA5

    doi: 10.5021/ad.2012.24.4.444

    Figure Lengend Snippet: PCR-RFLP patterns of 26S rDNA PCR digested with restriction enzymes (A) Hha I, (B) BtsC I of 11 Malassezia standard strains in hypopigmented lesions of 9 patients. Lanes: M: molecular Marker , 1: Malassezia slooffiae (KCTC17431), 2: M. globosa (CBS7966), 3: M. restricta (KCTC7848), 4: M. restricta (KCTC7848), 5: M. globosa (CBS7966), 6: M. globosa (CBS7966), 7: M. globosa (CBS7966), 8: M. sympodialis (KCTC7985), 9: M. furfur (KCTC7743). PCR: polymerase chain reaction, RFLP: restriction fragment length polymorphism.

    Article Snippet: Two restriction enzymes, Hha I (Koschem, Seoul, Korea) and BtsC I (NEB, London, England) were used to perform 26S rDNA-RFLP of Malassezia .

    Techniques: Polymerase Chain Reaction, Marker

    Dominance of the inoculated strains after SSF as revealed by their RFLP profiles. The RFLP profiles from the 16S rRNA genes of the selected strains (S7, CR18 and T5) were generated using a culture-independent approach. DNA was extracted directly from FSBM (inoculated and non-Inoculated groups), and the PCR amplicons were digested with the restriction enzymes Bmt I and BtsC I. The figure shows the RFLPs of the selected strains (S7, CR18 and T5) and the RFLP profiles from five replicates of inoculated and non-inoculated SSFs. The arrows indicate the presence of the S7 and T5 strains as dominant in the fermentation process.

    Journal: PLoS ONE

    Article Title: Reduction of Soybean Meal Non-Starch Polysaccharides and ?-Galactosides by Solid-State Fermentation Using Cellulolytic Bacteria Obtained from Different Environments

    doi: 10.1371/journal.pone.0044783

    Figure Lengend Snippet: Dominance of the inoculated strains after SSF as revealed by their RFLP profiles. The RFLP profiles from the 16S rRNA genes of the selected strains (S7, CR18 and T5) were generated using a culture-independent approach. DNA was extracted directly from FSBM (inoculated and non-Inoculated groups), and the PCR amplicons were digested with the restriction enzymes Bmt I and BtsC I. The figure shows the RFLPs of the selected strains (S7, CR18 and T5) and the RFLP profiles from five replicates of inoculated and non-inoculated SSFs. The arrows indicate the presence of the S7 and T5 strains as dominant in the fermentation process.

    Article Snippet: The amplicons were then digested by the restriction enzymes Bmt I and BtsC I (New England BioLabs).

    Techniques: Generated, Polymerase Chain Reaction