btsc i  (New England Biolabs)


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    Name:
    BtsCI
    Description:
    BtsCI 2 000 units
    Catalog Number:
    r0647l
    Price:
    70
    Size:
    2 000 units
    Category:
    Restriction Enzymes
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    Structured Review

    New England Biolabs btsc i
    BtsCI
    BtsCI 2 000 units
    https://www.bioz.com/result/btsc i/product/New England Biolabs
    Average 94 stars, based on 31 article reviews
    Price from $9.99 to $1999.99
    btsc i - by Bioz Stars, 2020-11
    94/100 stars

    Images

    1) Product Images from "Skin Characteristics in Patients with Pityriasis Versicolor Using Non-Invasive Method, MPA5"

    Article Title: Skin Characteristics in Patients with Pityriasis Versicolor Using Non-Invasive Method, MPA5

    Journal: Annals of Dermatology

    doi: 10.5021/ad.2012.24.4.444

    PCR-RFLP patterns of 26S rDNA PCR digested with restriction enzymes (A) Hha I, (B) BtsC I of 11 Malassezia standard strains in hyperpigmented lesions of 21 patients. Lanes: M: molecular Marker, 1: Malassezia globosa (CBS7966), 2: M. globosa (CBS7966), 3: M. restricta (KCTC7848), 4: M. globosa (CBS7966), 5: M. restricta (KCTC7848), 6: M. restricta (KCTC7848), 7: M. restricta (KCTC7848), 8: M. slooffiae (KCTC17431), 9: M. globosa (CBS7966), 10: M. globosa (CBS7966), 11: M. restricta (KCTC7848), 12: M. globosa (CBS7966), 13: M. globosa (CBS7966), 14: M. furfur (KCTC7743), 15: M. slooffiae (KCTC17431), 16: M. globosa (CBS7966), 17: M. globosa (CBS7966), 18: M. restricta (KCTC7848), 19: M. restricta (KCTC7848), 20: M. furfur (KCTC7743), 21: M. sympodialis (KCTC7985). PCR: polymerase chain reaction, RFLP: restriction fragment length polymorphism.
    Figure Legend Snippet: PCR-RFLP patterns of 26S rDNA PCR digested with restriction enzymes (A) Hha I, (B) BtsC I of 11 Malassezia standard strains in hyperpigmented lesions of 21 patients. Lanes: M: molecular Marker, 1: Malassezia globosa (CBS7966), 2: M. globosa (CBS7966), 3: M. restricta (KCTC7848), 4: M. globosa (CBS7966), 5: M. restricta (KCTC7848), 6: M. restricta (KCTC7848), 7: M. restricta (KCTC7848), 8: M. slooffiae (KCTC17431), 9: M. globosa (CBS7966), 10: M. globosa (CBS7966), 11: M. restricta (KCTC7848), 12: M. globosa (CBS7966), 13: M. globosa (CBS7966), 14: M. furfur (KCTC7743), 15: M. slooffiae (KCTC17431), 16: M. globosa (CBS7966), 17: M. globosa (CBS7966), 18: M. restricta (KCTC7848), 19: M. restricta (KCTC7848), 20: M. furfur (KCTC7743), 21: M. sympodialis (KCTC7985). PCR: polymerase chain reaction, RFLP: restriction fragment length polymorphism.

    Techniques Used: Polymerase Chain Reaction, Marker

    PCR-RFLP patterns of 26S rDNA PCR digested with restriction enzymes (A) Hha I, (B) BtsC I of 11 Malassezia standard strains in hypopigmented lesions of 9 patients. Lanes: M: molecular Marker , 1: Malassezia slooffiae (KCTC17431), 2: M. globosa (CBS7966), 3: M. restricta (KCTC7848), 4: M. restricta (KCTC7848), 5: M. globosa (CBS7966), 6: M. globosa (CBS7966), 7: M. globosa (CBS7966), 8: M. sympodialis (KCTC7985), 9: M. furfur (KCTC7743). PCR: polymerase chain reaction, RFLP: restriction fragment length polymorphism.
    Figure Legend Snippet: PCR-RFLP patterns of 26S rDNA PCR digested with restriction enzymes (A) Hha I, (B) BtsC I of 11 Malassezia standard strains in hypopigmented lesions of 9 patients. Lanes: M: molecular Marker , 1: Malassezia slooffiae (KCTC17431), 2: M. globosa (CBS7966), 3: M. restricta (KCTC7848), 4: M. restricta (KCTC7848), 5: M. globosa (CBS7966), 6: M. globosa (CBS7966), 7: M. globosa (CBS7966), 8: M. sympodialis (KCTC7985), 9: M. furfur (KCTC7743). PCR: polymerase chain reaction, RFLP: restriction fragment length polymorphism.

    Techniques Used: Polymerase Chain Reaction, Marker

    2) Product Images from "Mice expressing a human KATP channel mutation have altered channel ATP sensitivity but no cardiac abnormalities"

    Article Title: Mice expressing a human KATP channel mutation have altered channel ATP sensitivity but no cardiac abnormalities

    Journal: Diabetologia

    doi: 10.1007/s00125-011-2428-6

    Kcnj11 expression in the heart. a Kcnj11 expression in tissue isolated from ROSA, WT, Mck-Cre and m-V59M mice. WT, but not mutant (V59M), Kcnj11 cDNA is cut by the restriction enzyme BtsCI: the presence of two bands thus indicates the presence of the WT gene only and three bands indicates both WT and mutant genes. Data are representative of experiments on seven ROSA, four m-V59M, four WT and four Mck-Cre mice. sk1, quadriceps muscle; sk2, triceps muscle; sk3, diaphragm. b,c Quantitative PCR showing expression of Kcnj11 in heart and brain ( b ) and Gfp in heart ( c ) of control (grey bars, n = 5) and m-V59M mice (white bars, n = 5), relative to a panel of housekeeping genes. * p
    Figure Legend Snippet: Kcnj11 expression in the heart. a Kcnj11 expression in tissue isolated from ROSA, WT, Mck-Cre and m-V59M mice. WT, but not mutant (V59M), Kcnj11 cDNA is cut by the restriction enzyme BtsCI: the presence of two bands thus indicates the presence of the WT gene only and three bands indicates both WT and mutant genes. Data are representative of experiments on seven ROSA, four m-V59M, four WT and four Mck-Cre mice. sk1, quadriceps muscle; sk2, triceps muscle; sk3, diaphragm. b,c Quantitative PCR showing expression of Kcnj11 in heart and brain ( b ) and Gfp in heart ( c ) of control (grey bars, n = 5) and m-V59M mice (white bars, n = 5), relative to a panel of housekeeping genes. * p

    Techniques Used: Expressing, Isolation, Mouse Assay, Mutagenesis, Real-time Polymerase Chain Reaction

    3) Product Images from "Reduction of Soybean Meal Non-Starch Polysaccharides and ?-Galactosides by Solid-State Fermentation Using Cellulolytic Bacteria Obtained from Different Environments"

    Article Title: Reduction of Soybean Meal Non-Starch Polysaccharides and ?-Galactosides by Solid-State Fermentation Using Cellulolytic Bacteria Obtained from Different Environments

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0044783

    Dominance of the inoculated strains after SSF as revealed by their RFLP profiles. The RFLP profiles from the 16S rRNA genes of the selected strains (S7, CR18 and T5) were generated using a culture-independent approach. DNA was extracted directly from FSBM (inoculated and non-Inoculated groups), and the PCR amplicons were digested with the restriction enzymes Bmt I and BtsC I. The figure shows the RFLPs of the selected strains (S7, CR18 and T5) and the RFLP profiles from five replicates of inoculated and non-inoculated SSFs. The arrows indicate the presence of the S7 and T5 strains as dominant in the fermentation process.
    Figure Legend Snippet: Dominance of the inoculated strains after SSF as revealed by their RFLP profiles. The RFLP profiles from the 16S rRNA genes of the selected strains (S7, CR18 and T5) were generated using a culture-independent approach. DNA was extracted directly from FSBM (inoculated and non-Inoculated groups), and the PCR amplicons were digested with the restriction enzymes Bmt I and BtsC I. The figure shows the RFLPs of the selected strains (S7, CR18 and T5) and the RFLP profiles from five replicates of inoculated and non-inoculated SSFs. The arrows indicate the presence of the S7 and T5 strains as dominant in the fermentation process.

    Techniques Used: Generated, Polymerase Chain Reaction

    4) Product Images from "The Moraxella catarrhalis phase-variable DNA methyltransferase ModM3 is an epigenetic regulator that affects bacterial survival in an in vivo model of otitis media"

    Article Title: The Moraxella catarrhalis phase-variable DNA methyltransferase ModM3 is an epigenetic regulator that affects bacterial survival in an in vivo model of otitis media

    Journal: BMC Microbiology

    doi: 10.1186/s12866-019-1660-y

    Southern blotting confirms the ModM3 methylation target sequence 5′-AC m6 ATC-3′. a Schematic representation of the restriction-inhibition assay used to confirm the ModM3 methylation site. The location of the Southern blot probe, and the BtsCI and ModM3 recognition sites are shown. The central BtsCI site overlaps the ModM3 recognition sequence and is sensitive to overlapping N6-methyladenine methylation. b Restriction-inhibition assay of modM3 ON, modM3 OFF and ∆ modM3 genomic DNA using the methyl-sensitive restriction endonuclease BtsCI. The restriction endonuclease HindIII is not sensitive to methylation and is included as a control for digestion. c Southern blot of BtsCI digested genomic DNA isolated from modM3 ON, modM3 OFF, and ∆ modM3 strains. Methylated DNA in the ModM3 ON strain is protected from BtsCI digestion resulting in a 1.5 kb band. All BtsCI sites are cleaved in the modM3 OFF and ∆ modM3 strains as ModM3 methylation is absent, resulting in a 0.5 kb band. d qRT-PCR indicating the relative abundance of methylated, undigested genomic DNA in modM3 ON and modM3 OFF relative to ∆ modM3 following digestion with BtsCI (Ct values of 18.32, 21.97, 24.77, respectively, normalised to copB reference)
    Figure Legend Snippet: Southern blotting confirms the ModM3 methylation target sequence 5′-AC m6 ATC-3′. a Schematic representation of the restriction-inhibition assay used to confirm the ModM3 methylation site. The location of the Southern blot probe, and the BtsCI and ModM3 recognition sites are shown. The central BtsCI site overlaps the ModM3 recognition sequence and is sensitive to overlapping N6-methyladenine methylation. b Restriction-inhibition assay of modM3 ON, modM3 OFF and ∆ modM3 genomic DNA using the methyl-sensitive restriction endonuclease BtsCI. The restriction endonuclease HindIII is not sensitive to methylation and is included as a control for digestion. c Southern blot of BtsCI digested genomic DNA isolated from modM3 ON, modM3 OFF, and ∆ modM3 strains. Methylated DNA in the ModM3 ON strain is protected from BtsCI digestion resulting in a 1.5 kb band. All BtsCI sites are cleaved in the modM3 OFF and ∆ modM3 strains as ModM3 methylation is absent, resulting in a 0.5 kb band. d qRT-PCR indicating the relative abundance of methylated, undigested genomic DNA in modM3 ON and modM3 OFF relative to ∆ modM3 following digestion with BtsCI (Ct values of 18.32, 21.97, 24.77, respectively, normalised to copB reference)

    Techniques Used: Southern Blot, Methylation, Sequencing, Inhibition, Isolation, Quantitative RT-PCR

    Related Articles

    Agarose Gel Electrophoresis:

    Article Title: Restoration of Vision in the pde6?-deficient Dog, a Large Animal Model of Rod-cone Dystrophy
    Article Snippet: .. Pde6β PCR products were purified by Nucleospin Extract II (Macherey-Nagel, Hoerdt, France) before digestion by BtsCI (New England Biolabs, Ipswich, MA) for 2 hours at 50 °C according to the manufacturer's instructions and analyzed by 3.5% agarose gel electrophoresis. ..

    Article Title: Expression of an activating mutation in the gene encoding the KATP channel subunit Kir6.2 in mouse pancreatic ? cells recapitulates neonatal diabetes
    Article Snippet: .. After 40 cycles (95°C for 30 seconds; 60°C for 30 seconds; 72°C for 1 minute), PCR products were digested with BtsCI restriction enzyme (New England BioLabs) for approximately 1 hour at 50°C and digested PCR fragments visualized on a 2% agarose gel. .. Sequences of primers used for GFP amplification were as follows: forward, 5′-GAGGTGAAGTTCGAGGGCGAC-3′; and reverse, 5′-CAGGACCATGTGATCGCGCTT-3′.

    Purification:

    Article Title: Restoration of Vision in the pde6?-deficient Dog, a Large Animal Model of Rod-cone Dystrophy
    Article Snippet: .. Pde6β PCR products were purified by Nucleospin Extract II (Macherey-Nagel, Hoerdt, France) before digestion by BtsCI (New England Biolabs, Ipswich, MA) for 2 hours at 50 °C according to the manufacturer's instructions and analyzed by 3.5% agarose gel electrophoresis. ..

    Article Title: Programming molecular topologies from single-stranded nucleic acids
    Article Snippet: .. The ssDNAs were cleaved off from the recombinant phage DNAs by using a BtsCI restriction enzyme (New England Biolabs), and were gel purified by using a 4% urea denaturing PAGE gel. ..

    Article Title: Folding complex DNA nanostructures from limited sets of reusable sequences
    Article Snippet: .. For the custom scaffold 24-helix bundle, the purified scaffold was subsequently digested with BtsCI (NEB, Catalog # R0647L) as follows: 10 μL of ssDNA at 100 nM with 2 μL of 10x CutSmart Buffer, 1 μL of BtsCI and 7 μL of ddH2 O was incubated at 50°C overnight. .. Note, that the final scaffold sequence will contain two dsDNA restriction sites for BtsCI (hairpin at each end, Supplementary Figure S2) and several ssDNA restriction sites for BtsCI.

    Incubation:

    Article Title: Folding complex DNA nanostructures from limited sets of reusable sequences
    Article Snippet: .. For the custom scaffold 24-helix bundle, the purified scaffold was subsequently digested with BtsCI (NEB, Catalog # R0647L) as follows: 10 μL of ssDNA at 100 nM with 2 μL of 10x CutSmart Buffer, 1 μL of BtsCI and 7 μL of ddH2 O was incubated at 50°C overnight. .. Note, that the final scaffold sequence will contain two dsDNA restriction sites for BtsCI (hairpin at each end, Supplementary Figure S2) and several ssDNA restriction sites for BtsCI.

    Polyacrylamide Gel Electrophoresis:

    Article Title: Programming molecular topologies from single-stranded nucleic acids
    Article Snippet: .. The ssDNAs were cleaved off from the recombinant phage DNAs by using a BtsCI restriction enzyme (New England Biolabs), and were gel purified by using a 4% urea denaturing PAGE gel. ..

    Polymerase Chain Reaction:

    Article Title: Restoration of Vision in the pde6?-deficient Dog, a Large Animal Model of Rod-cone Dystrophy
    Article Snippet: .. Pde6β PCR products were purified by Nucleospin Extract II (Macherey-Nagel, Hoerdt, France) before digestion by BtsCI (New England Biolabs, Ipswich, MA) for 2 hours at 50 °C according to the manufacturer's instructions and analyzed by 3.5% agarose gel electrophoresis. ..

    Article Title: Mice expressing a human KATP channel mutation have altered channel ATP sensitivity but no cardiac abnormalities
    Article Snippet: .. PCR products were digested with BtsCI (New England Biolabs, Hitchin, UK) for ∼2 h at 50°C. .. Digested PCR fragments were visualised on an agarose gel with ethidium bromide.

    Article Title: Expression of an activating mutation in the gene encoding the KATP channel subunit Kir6.2 in mouse pancreatic ? cells recapitulates neonatal diabetes
    Article Snippet: .. After 40 cycles (95°C for 30 seconds; 60°C for 30 seconds; 72°C for 1 minute), PCR products were digested with BtsCI restriction enzyme (New England BioLabs) for approximately 1 hour at 50°C and digested PCR fragments visualized on a 2% agarose gel. .. Sequences of primers used for GFP amplification were as follows: forward, 5′-GAGGTGAAGTTCGAGGGCGAC-3′; and reverse, 5′-CAGGACCATGTGATCGCGCTT-3′.

    Recombinant:

    Article Title: Programming molecular topologies from single-stranded nucleic acids
    Article Snippet: .. The ssDNAs were cleaved off from the recombinant phage DNAs by using a BtsCI restriction enzyme (New England Biolabs), and were gel purified by using a 4% urea denaturing PAGE gel. ..

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  • 94
    New England Biolabs btsc i
    PCR-RFLP patterns of 26S rDNA PCR digested with restriction enzymes (A) <t>Hha</t> I, (B) <t>BtsC</t> I of 11 Malassezia standard strains in hyperpigmented lesions of 21 patients. Lanes: M: molecular Marker, 1: Malassezia globosa (CBS7966), 2: M. globosa (CBS7966), 3: M. restricta (KCTC7848), 4: M. globosa (CBS7966), 5: M. restricta (KCTC7848), 6: M. restricta (KCTC7848), 7: M. restricta (KCTC7848), 8: M. slooffiae (KCTC17431), 9: M. globosa (CBS7966), 10: M. globosa (CBS7966), 11: M. restricta (KCTC7848), 12: M. globosa (CBS7966), 13: M. globosa (CBS7966), 14: M. furfur (KCTC7743), 15: M. slooffiae (KCTC17431), 16: M. globosa (CBS7966), 17: M. globosa (CBS7966), 18: M. restricta (KCTC7848), 19: M. restricta (KCTC7848), 20: M. furfur (KCTC7743), 21: M. sympodialis (KCTC7985). PCR: polymerase chain reaction, RFLP: restriction fragment length polymorphism.
    Btsc I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 21 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/btsc i/product/New England Biolabs
    Average 94 stars, based on 21 article reviews
    Price from $9.99 to $1999.99
    btsc i - by Bioz Stars, 2020-11
    94/100 stars
      Buy from Supplier

    Image Search Results


    PCR-RFLP patterns of 26S rDNA PCR digested with restriction enzymes (A) Hha I, (B) BtsC I of 11 Malassezia standard strains in hyperpigmented lesions of 21 patients. Lanes: M: molecular Marker, 1: Malassezia globosa (CBS7966), 2: M. globosa (CBS7966), 3: M. restricta (KCTC7848), 4: M. globosa (CBS7966), 5: M. restricta (KCTC7848), 6: M. restricta (KCTC7848), 7: M. restricta (KCTC7848), 8: M. slooffiae (KCTC17431), 9: M. globosa (CBS7966), 10: M. globosa (CBS7966), 11: M. restricta (KCTC7848), 12: M. globosa (CBS7966), 13: M. globosa (CBS7966), 14: M. furfur (KCTC7743), 15: M. slooffiae (KCTC17431), 16: M. globosa (CBS7966), 17: M. globosa (CBS7966), 18: M. restricta (KCTC7848), 19: M. restricta (KCTC7848), 20: M. furfur (KCTC7743), 21: M. sympodialis (KCTC7985). PCR: polymerase chain reaction, RFLP: restriction fragment length polymorphism.

    Journal: Annals of Dermatology

    Article Title: Skin Characteristics in Patients with Pityriasis Versicolor Using Non-Invasive Method, MPA5

    doi: 10.5021/ad.2012.24.4.444

    Figure Lengend Snippet: PCR-RFLP patterns of 26S rDNA PCR digested with restriction enzymes (A) Hha I, (B) BtsC I of 11 Malassezia standard strains in hyperpigmented lesions of 21 patients. Lanes: M: molecular Marker, 1: Malassezia globosa (CBS7966), 2: M. globosa (CBS7966), 3: M. restricta (KCTC7848), 4: M. globosa (CBS7966), 5: M. restricta (KCTC7848), 6: M. restricta (KCTC7848), 7: M. restricta (KCTC7848), 8: M. slooffiae (KCTC17431), 9: M. globosa (CBS7966), 10: M. globosa (CBS7966), 11: M. restricta (KCTC7848), 12: M. globosa (CBS7966), 13: M. globosa (CBS7966), 14: M. furfur (KCTC7743), 15: M. slooffiae (KCTC17431), 16: M. globosa (CBS7966), 17: M. globosa (CBS7966), 18: M. restricta (KCTC7848), 19: M. restricta (KCTC7848), 20: M. furfur (KCTC7743), 21: M. sympodialis (KCTC7985). PCR: polymerase chain reaction, RFLP: restriction fragment length polymorphism.

    Article Snippet: Two restriction enzymes, Hha I (Koschem, Seoul, Korea) and BtsC I (NEB, London, England) were used to perform 26S rDNA-RFLP of Malassezia .

    Techniques: Polymerase Chain Reaction, Marker

    PCR-RFLP patterns of 26S rDNA PCR digested with restriction enzymes (A) Hha I, (B) BtsC I of 11 Malassezia standard strains in hypopigmented lesions of 9 patients. Lanes: M: molecular Marker , 1: Malassezia slooffiae (KCTC17431), 2: M. globosa (CBS7966), 3: M. restricta (KCTC7848), 4: M. restricta (KCTC7848), 5: M. globosa (CBS7966), 6: M. globosa (CBS7966), 7: M. globosa (CBS7966), 8: M. sympodialis (KCTC7985), 9: M. furfur (KCTC7743). PCR: polymerase chain reaction, RFLP: restriction fragment length polymorphism.

    Journal: Annals of Dermatology

    Article Title: Skin Characteristics in Patients with Pityriasis Versicolor Using Non-Invasive Method, MPA5

    doi: 10.5021/ad.2012.24.4.444

    Figure Lengend Snippet: PCR-RFLP patterns of 26S rDNA PCR digested with restriction enzymes (A) Hha I, (B) BtsC I of 11 Malassezia standard strains in hypopigmented lesions of 9 patients. Lanes: M: molecular Marker , 1: Malassezia slooffiae (KCTC17431), 2: M. globosa (CBS7966), 3: M. restricta (KCTC7848), 4: M. restricta (KCTC7848), 5: M. globosa (CBS7966), 6: M. globosa (CBS7966), 7: M. globosa (CBS7966), 8: M. sympodialis (KCTC7985), 9: M. furfur (KCTC7743). PCR: polymerase chain reaction, RFLP: restriction fragment length polymorphism.

    Article Snippet: Two restriction enzymes, Hha I (Koschem, Seoul, Korea) and BtsC I (NEB, London, England) were used to perform 26S rDNA-RFLP of Malassezia .

    Techniques: Polymerase Chain Reaction, Marker

    Kcnj11 expression in the heart. a Kcnj11 expression in tissue isolated from ROSA, WT, Mck-Cre and m-V59M mice. WT, but not mutant (V59M), Kcnj11 cDNA is cut by the restriction enzyme BtsCI: the presence of two bands thus indicates the presence of the WT gene only and three bands indicates both WT and mutant genes. Data are representative of experiments on seven ROSA, four m-V59M, four WT and four Mck-Cre mice. sk1, quadriceps muscle; sk2, triceps muscle; sk3, diaphragm. b,c Quantitative PCR showing expression of Kcnj11 in heart and brain ( b ) and Gfp in heart ( c ) of control (grey bars, n = 5) and m-V59M mice (white bars, n = 5), relative to a panel of housekeeping genes. * p

    Journal: Diabetologia

    Article Title: Mice expressing a human KATP channel mutation have altered channel ATP sensitivity but no cardiac abnormalities

    doi: 10.1007/s00125-011-2428-6

    Figure Lengend Snippet: Kcnj11 expression in the heart. a Kcnj11 expression in tissue isolated from ROSA, WT, Mck-Cre and m-V59M mice. WT, but not mutant (V59M), Kcnj11 cDNA is cut by the restriction enzyme BtsCI: the presence of two bands thus indicates the presence of the WT gene only and three bands indicates both WT and mutant genes. Data are representative of experiments on seven ROSA, four m-V59M, four WT and four Mck-Cre mice. sk1, quadriceps muscle; sk2, triceps muscle; sk3, diaphragm. b,c Quantitative PCR showing expression of Kcnj11 in heart and brain ( b ) and Gfp in heart ( c ) of control (grey bars, n = 5) and m-V59M mice (white bars, n = 5), relative to a panel of housekeeping genes. * p

    Article Snippet: PCR products were digested with BtsCI (New England Biolabs, Hitchin, UK) for ∼2 h at 50°C.

    Techniques: Expressing, Isolation, Mouse Assay, Mutagenesis, Real-time Polymerase Chain Reaction

    Dominance of the inoculated strains after SSF as revealed by their RFLP profiles. The RFLP profiles from the 16S rRNA genes of the selected strains (S7, CR18 and T5) were generated using a culture-independent approach. DNA was extracted directly from FSBM (inoculated and non-Inoculated groups), and the PCR amplicons were digested with the restriction enzymes Bmt I and BtsC I. The figure shows the RFLPs of the selected strains (S7, CR18 and T5) and the RFLP profiles from five replicates of inoculated and non-inoculated SSFs. The arrows indicate the presence of the S7 and T5 strains as dominant in the fermentation process.

    Journal: PLoS ONE

    Article Title: Reduction of Soybean Meal Non-Starch Polysaccharides and ?-Galactosides by Solid-State Fermentation Using Cellulolytic Bacteria Obtained from Different Environments

    doi: 10.1371/journal.pone.0044783

    Figure Lengend Snippet: Dominance of the inoculated strains after SSF as revealed by their RFLP profiles. The RFLP profiles from the 16S rRNA genes of the selected strains (S7, CR18 and T5) were generated using a culture-independent approach. DNA was extracted directly from FSBM (inoculated and non-Inoculated groups), and the PCR amplicons were digested with the restriction enzymes Bmt I and BtsC I. The figure shows the RFLPs of the selected strains (S7, CR18 and T5) and the RFLP profiles from five replicates of inoculated and non-inoculated SSFs. The arrows indicate the presence of the S7 and T5 strains as dominant in the fermentation process.

    Article Snippet: The amplicons were then digested by the restriction enzymes Bmt I and BtsC I (New England BioLabs).

    Techniques: Generated, Polymerase Chain Reaction

    Southern blotting confirms the ModM3 methylation target sequence 5′-AC m6 ATC-3′. a Schematic representation of the restriction-inhibition assay used to confirm the ModM3 methylation site. The location of the Southern blot probe, and the BtsCI and ModM3 recognition sites are shown. The central BtsCI site overlaps the ModM3 recognition sequence and is sensitive to overlapping N6-methyladenine methylation. b Restriction-inhibition assay of modM3 ON, modM3 OFF and ∆ modM3 genomic DNA using the methyl-sensitive restriction endonuclease BtsCI. The restriction endonuclease HindIII is not sensitive to methylation and is included as a control for digestion. c Southern blot of BtsCI digested genomic DNA isolated from modM3 ON, modM3 OFF, and ∆ modM3 strains. Methylated DNA in the ModM3 ON strain is protected from BtsCI digestion resulting in a 1.5 kb band. All BtsCI sites are cleaved in the modM3 OFF and ∆ modM3 strains as ModM3 methylation is absent, resulting in a 0.5 kb band. d qRT-PCR indicating the relative abundance of methylated, undigested genomic DNA in modM3 ON and modM3 OFF relative to ∆ modM3 following digestion with BtsCI (Ct values of 18.32, 21.97, 24.77, respectively, normalised to copB reference)

    Journal: BMC Microbiology

    Article Title: The Moraxella catarrhalis phase-variable DNA methyltransferase ModM3 is an epigenetic regulator that affects bacterial survival in an in vivo model of otitis media

    doi: 10.1186/s12866-019-1660-y

    Figure Lengend Snippet: Southern blotting confirms the ModM3 methylation target sequence 5′-AC m6 ATC-3′. a Schematic representation of the restriction-inhibition assay used to confirm the ModM3 methylation site. The location of the Southern blot probe, and the BtsCI and ModM3 recognition sites are shown. The central BtsCI site overlaps the ModM3 recognition sequence and is sensitive to overlapping N6-methyladenine methylation. b Restriction-inhibition assay of modM3 ON, modM3 OFF and ∆ modM3 genomic DNA using the methyl-sensitive restriction endonuclease BtsCI. The restriction endonuclease HindIII is not sensitive to methylation and is included as a control for digestion. c Southern blot of BtsCI digested genomic DNA isolated from modM3 ON, modM3 OFF, and ∆ modM3 strains. Methylated DNA in the ModM3 ON strain is protected from BtsCI digestion resulting in a 1.5 kb band. All BtsCI sites are cleaved in the modM3 OFF and ∆ modM3 strains as ModM3 methylation is absent, resulting in a 0.5 kb band. d qRT-PCR indicating the relative abundance of methylated, undigested genomic DNA in modM3 ON and modM3 OFF relative to ∆ modM3 following digestion with BtsCI (Ct values of 18.32, 21.97, 24.77, respectively, normalised to copB reference)

    Article Snippet: Genomic DNA was digested with the restriction enzyme BtsCI (NEB) at 50 °C or HindIII (NEB) at 37 °C for 60 min.

    Techniques: Southern Blot, Methylation, Sequencing, Inhibition, Isolation, Quantitative RT-PCR