sbfi (New England Biolabs)
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Name:
SbfI
Description:
SbfI 2 500 units
Catalog Number:
r0642l
Price:
302
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2 500 units
Category:
Restriction Enzymes
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Structured Review
New England Biolabs
sbfi
SbfI 2 500 units
https://www.bioz.com/result/sbfi/product/New England Biolabs
Average 95 stars, based on 43 article reviews
Price from $9.99 to $1999.99
SbfI 2 500 units
https://www.bioz.com/result/sbfi/product/New England Biolabs
Average 95 stars, based on 43 article reviews
Price from $9.99 to $1999.99
sbfi - by Bioz Stars,
2020-02
95/100 stars
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Images
1) Product Images from "Functional metagenomics identifies an exosialidase with an inverting catalytic mechanism that defines a new glycoside hydrolase family (GH156)"
Article Title: Functional metagenomics identifies an exosialidase with an inverting catalytic mechanism that defines a new glycoside hydrolase family (GH156)
Journal: The Journal of Biological Chemistry
doi: 10.1074/jbc.RA118.003302
Figure Legend Snippet: Screening for sialidase activity from a hot spring metagenomic library. A , restriction fragment analysis of 12 randomly selected clones from the hot spring metagenomic library were isolated and digested with the rare-cutting endonuclease SbfI. Digested fosmids were separated overnight on a 1% agarose gel along with a λHindIII size marker and a linearized pSMART FOS empty vector control ( lane 14 ; contains one SbfI site). Each clone showed a unique banding pattern, with fragments whose combined sizes indicated the presence of an insert of at least 30–40 kb. B , E. coli cells harboring individual fosmid clones were assayed for sialidase activity with X-Neu5Ac incorporated into agar medium. A single positive clone forming a blue colony is denoted with an arrow. C , lysate from microcultures of E. coli cells harboring individual fosmid clones were assayed for sialidase activity with 4MU-α-Neu5Ac. A single positive clone is denoted with an arrow .
Techniques Used: Activity Assay, Clone Assay, Isolation, Agarose Gel Electrophoresis, Marker, Plasmid Preparation
2) Product Images from "Amplification Biases and Consistent Recovery of Loci in a Double-Digest RAD-seq Protocol"
Article Title: Amplification Biases and Consistent Recovery of Loci in a Double-Digest RAD-seq Protocol
Journal: PLoS ONE
doi: 10.1371/journal.pone.0106713
Figure Legend Snippet: Sequencing depth for single copy ddRAD loci in relation to the corresponding sequence in the zebra finch reference genome. Categories from top to bottom include: loci mapping as expected to predicted SbfI-EcoRI restriction fragments≤328 bp in length; all loci beginning at a genomic location similar but not identical to the canonical SbfI recognition sequence (1–4 mismatches); subset of loci with one mismatch in position 1 or 8 of the SbfI recognition sequence; subset of loci with one mismatch in positions 2 through 7 of the SbfI recognition sequence; loci mapping to a genomic SbfI site without an EcoRI site within 328 bp; and loci mapping to a predicted SbfI-SbfI restriction fragment less than 328 bp in length.
Techniques Used: Sequencing
Figure Legend Snippet: Recovery and sequencing depth for predicted, single-copy ddRAD loci in the empirical zebra finch data. (A) Proportion of predicted loci recovered at three different minimum depth thresholds as a function of predicted fragment length. Each data point represents the proportion of ∼140–220 predicted loci recovered in a given 10 bp size range. Dashed vertical lines represent the upper and lower bounds of the size range isolated from the agarose gel. (B) Sequencing depth for recovered (depth ≥1), single-copy loci in the 32–500 bp size range (includes 5,232 of 5,783 predicted loci in the 38–328 bp size range). (C) The relationship between GC content and sequencing depth for zebra finch ddRAD loci. Data are shown for predicted, single-copy loci recovered at a depth ≥1 in three selected subsets of the overall size range ( n = 502, 466, and 445 loci in the 100–125, 200–225, and 300–325 bp size ranges, respectively). The predicted length and GC content of each locus are based on the full-length fragment in the reference genome, inclusive of the SbfI and EcoRI restriction sites on either end. Note that the y-axis is on a logarithmic scale in (B) and (C).
Techniques Used: Sequencing, Isolation, Agarose Gel Electrophoresis
3) Product Images from "Functional metagenomics identifies an exosialidase with an inverting catalytic mechanism that defines a new glycoside hydrolase family (GH156)"
Article Title: Functional metagenomics identifies an exosialidase with an inverting catalytic mechanism that defines a new glycoside hydrolase family (GH156)
Journal: The Journal of Biological Chemistry
doi: 10.1074/jbc.RA118.003302
Figure Legend Snippet: Screening for sialidase activity from a hot spring metagenomic library. A , restriction fragment analysis of 12 randomly selected clones from the hot spring metagenomic library were isolated and digested with the rare-cutting endonuclease SbfI. Digested fosmids were separated overnight on a 1% agarose gel along with a λHindIII size marker and a linearized pSMART FOS empty vector control ( lane 14 ; contains one SbfI site). Each clone showed a unique banding pattern, with fragments whose combined sizes indicated the presence of an insert of at least 30–40 kb. B , E. coli cells harboring individual fosmid clones were assayed for sialidase activity with X-Neu5Ac incorporated into agar medium. A single positive clone forming a blue colony is denoted with an arrow. C , lysate from microcultures of E. coli cells harboring individual fosmid clones were assayed for sialidase activity with 4MU-α-Neu5Ac. A single positive clone is denoted with an arrow .
Techniques Used: Activity Assay, Clone Assay, Isolation, Agarose Gel Electrophoresis, Marker, Plasmid Preparation
Related Articles
Clone Assay:Article Title: Characterization of the Rat Oncostatin M Receptor Complex Which Resembles the Human, but Differs from the Murine Cytokine Receptor Article Snippet: To generate tetracycline-inducible bidirectional promoter driven expression plasmids encoding the rgp130/rLIFR combination or the rgp130/rOSMR combination, we first cloned the cDNAs for each receptor using total RNA extractions from JTC-27 rat hepatoma cells. .. The rOSMR and rLIFR amplicons were digested with Amplification:Article Title: Characterization of the Rat Oncostatin M Receptor Complex Which Resembles the Human, but Differs from the Murine Cytokine Receptor Article Snippet: The rgp130 amplicon was digested with Age I and Not I fast digest enzymes (Fermentas) for 30 minutes at 37°C. .. The rOSMR and rLIFR amplicons were digested with Article Title: Mapping and Validation of the Major Sex-Determining Region in Nile Tilapia (Oreochromis niloticus L.) Using RAD Sequencing Article Snippet: The RAD specific P1 and P2 paired-end adapters and library amplification PCR primer sequences used in this study are detailed in Baxter et al. . .. Each sample (0.72 µg parental DNA/0.24 µg offspring DNA) was digested at 37°C for 40 minutes with Article Title: Accuracy of Genomic Evaluations of Juvenile Growth Rate in Common Carp (Cyprinus carpio) Using Genotyping by Sequencing Article Snippet: The RAD-specific P1 and P2 paired-end adapters and library amplification PCR primer sequences used in this study are detailed in . .. Briefly, each sample (0.72 μg parental DNA/0.24 μg offspring DNA) was digested at 37°C for 60 min with Article Title: Mapping the sex determination locus in the Atlantic halibut (Hippoglossus hippoglossus) using RAD sequencing Article Snippet: The RAD-specific P1 and P2 paired-end adapters and library amplification PCR primer sequences used in this study are detailed in Baxter et al. [ ]. .. Each sample (1.5 μg parental DNA / 0.5 μg offspring DNA) was digested at 37°C for 30 minutes with Article Title: Genome-wide signatures of local adaptation among seven stoneflies species along a nationwide latitudinal gradient in Japan Article Snippet: Briefly, 100 ng genomic DNA per sample was digested with restriction endonucleases, Article Title: Accuracy of Genomic Evaluations of Juvenile Growth Rate in Common Carp (Cyprinus carpio) Using Genotyping by Sequencing Article Snippet: The RAD-specific P1 and P2 paired-end adapters and library amplification PCR primer sequences used in this study are detailed in . .. Briefly, each sample (0.72 μg parental DNA/0.24 μg offspring DNA) was digested at 37°C for 60 min with Stable Transfection:Article Title: Characterization of the Rat Oncostatin M Receptor Complex Which Resembles the Human, but Differs from the Murine Cytokine Receptor Article Snippet: The rOSMR and rLIFR amplicons were digested with Construct:Article Title: Characterization of the Rat Oncostatin M Receptor Complex Which Resembles the Human, but Differs from the Murine Cytokine Receptor Article Snippet: The rOSMR and rLIFR amplicons were digested with Article Title: Long-term live imaging reveals cytosolic immune responses of host hepatocytes against Plasmodium infection and parasite escape mechanisms Article Snippet: .. Construct pL1720 was made as follows: first the selectable marker (SM) cassette from pL1661 (containing both 5′hsp70 and 3′hsp70 sequences, tgdhfr/ts SM, and 230p targeting regions) was removed by digesting the plasmid with Sna BI (Roche, 10997480001) and Article Title: Partners in Time: EARLY BIRD Associates with ZEITLUPE and Regulates the Speed of the Arabidopsis Clock 1Partners in Time: EARLY BIRD Associates with ZEITLUPE and Regulates the Speed of the Arabidopsis Clock 1 [W]Partners in Time: EARLY BIRD Associates with ZEITLUPE and Regulates the Speed of the Arabidopsis Clock 1 [W] [OA] Article Snippet: .. Complementation of the ebi-1 mutation was obtained by using the Myc-tagged CaMV 35S:EBI coding sequence in pRT104 plasmid (as described below), the construct was digested with Article Title: Genome-wide signatures of local adaptation among seven stoneflies species along a nationwide latitudinal gradient in Japan Article Snippet: This DNA extraction method involved the use of the whole body without any destruction of the specimen, thus reducing the probability of contamination (gut content contamination). ddRAD libraries were constructed as previously described [ ] with slight modifications. .. Briefly, 100 ng genomic DNA per sample was digested with restriction endonucleases, Incubation:Article Title: Mapping the sex determination locus in the hāpuku (Polyprion oxygeneios) using ddRAD sequencing Article Snippet: ddRAD library preparation and sequencing Following a modified version of the protocol described by Peterson et al. [ ], two ddRAD libraries were prepared using different pairs of high fidelity restriction enzymes (REs) (New England Biolabs, UK; NEB); one using Article Title: Gene-centromere mapping in meiotic gynogenetic European seabass Article Snippet: Each sample (40 ng DNA) was digested at 37 °C for 30 min with 0.8 U Article Title: Genome-wide signatures of local adaptation among seven stoneflies species along a nationwide latitudinal gradient in Japan Article Snippet: The whole body of the specimen was fully submerged in lysis buffer (Qiagen GmbH, Hilden, Germany) and incubated at 56 °C overnight. .. Briefly, 100 ng genomic DNA per sample was digested with restriction endonucleases, Article Title: Rapid SNP Discovery and Genetic Mapping Using Sequenced RAD Markers Article Snippet: Isolation of RAD markers for Illumina sequencing Genomic DNA (0.1–1 µg; from either individual or pooled samples) was digested for 15 min at 37°C in a 50 µL reaction with 20 units (U) of EcoR I or Luciferase:Article Title: Long-term live imaging reveals cytosolic immune responses of host hepatocytes against Plasmodium infection and parasite escape mechanisms Article Snippet: Generation and analysis of the reporter line PbmCherryhsp70 +Luceef1α Construct pL1720 was made for generation of reporter line mCherryhsp70 +Luceef1 α that expresses mCherry reporter under control of the hsp70 (PBANKA_071190) 5′- and 3′-regulatory sequences and luciferase under the control of the ee1f α (PBANKA_113330) 5′-promoter/regulatory sequences and the pbdhfr/ts (PBANKA_071930) 3′-regulatory sequences. .. Construct pL1720 was made as follows: first the selectable marker (SM) cassette from pL1661 (containing both 5′hsp70 and 3′hsp70 sequences, tgdhfr/ts SM, and 230p targeting regions) was removed by digesting the plasmid with Sna BI (Roche, 10997480001) and In Silico:Article Title: Gene-centromere mapping in meiotic gynogenetic European seabass Article Snippet: In silico estimation from the seabass genome predicted 52,230 ddRAD fragments with paired SbfI-SphI restriction site overhangs, while after the size selection applied in the present study (c. 320 bp −590 bp excluding adaptors) only 3603 fragments were predicted to be available. .. Each sample (40 ng DNA) was digested at 37 °C for 30 min with 0.8 U Expressing:Article Title: Characterization of the Rat Oncostatin M Receptor Complex Which Resembles the Human, but Differs from the Murine Cytokine Receptor Article Snippet: Paragraph title: Construction of expression vectors ... The rOSMR and rLIFR amplicons were digested with Article Title: Long-term live imaging reveals cytosolic immune responses of host hepatocytes against Plasmodium infection and parasite escape mechanisms Article Snippet: Construct pL1720 was made as follows: first the selectable marker (SM) cassette from pL1661 (containing both 5′hsp70 and 3′hsp70 sequences, tgdhfr/ts SM, and 230p targeting regions) was removed by digesting the plasmid with Sna BI (Roche, 10997480001) and Article Title: Partners in Time: EARLY BIRD Associates with ZEITLUPE and Regulates the Speed of the Arabidopsis Clock 1Partners in Time: EARLY BIRD Associates with ZEITLUPE and Regulates the Speed of the Arabidopsis Clock 1 [W]Partners in Time: EARLY BIRD Associates with ZEITLUPE and Regulates the Speed of the Arabidopsis Clock 1 [W] [OA] Article Snippet: ebi-1 was isolated from a screen of EMS-mutagenized M2 Arabidopsis ( Arabidopsis thaliana ) Ws-2 plants ( ) by its early phase of CAB2 :: LUC+ expression in DD. .. Complementation of the ebi-1 mutation was obtained by using the Myc-tagged CaMV 35S:EBI coding sequence in pRT104 plasmid (as described below), the construct was digested with Modification:Article Title: Mapping the sex determination locus in the hāpuku (Polyprion oxygeneios) using ddRAD sequencing Article Snippet: .. ddRAD library preparation and sequencing Following a modified version of the protocol described by Peterson et al. [ ], two ddRAD libraries were prepared using different pairs of high fidelity restriction enzymes (REs) (New England Biolabs, UK; NEB); one using Article Title: A novel sex-determining QTL in Nile tilapia (Oreochromis niloticus) Article Snippet: ddRAD library preparation and sequencing The ddRAD library preparation protocol followed a modified version of the methodology described by Peterson et al. [ ]. .. Each sample (0.1 μg DNA) was digested at 37°C for 40 minutes with Article Title: Rapid SNP Discovery and Genetic Mapping Using Sequenced RAD Markers Article Snippet: Isolation of RAD markers for Illumina sequencing Genomic DNA (0.1–1 µg; from either individual or pooled samples) was digested for 15 min at 37°C in a 50 µL reaction with 20 units (U) of EcoR I or Transformation Assay:Article Title: Partners in Time: EARLY BIRD Associates with ZEITLUPE and Regulates the Speed of the Arabidopsis Clock 1Partners in Time: EARLY BIRD Associates with ZEITLUPE and Regulates the Speed of the Arabidopsis Clock 1 [W]Partners in Time: EARLY BIRD Associates with ZEITLUPE and Regulates the Speed of the Arabidopsis Clock 1 [W] [OA] Article Snippet: Complementation of the ebi-1 mutation was obtained by using the Myc-tagged CaMV 35S:EBI coding sequence in pRT104 plasmid (as described below), the construct was digested with Gel Purification:Article Title: Characterization of the Rat Oncostatin M Receptor Complex Which Resembles the Human, but Differs from the Murine Cytokine Receptor Article Snippet: The rOSMR and rLIFR amplicons were digested with Article Title: Accuracy of Genomic Evaluations of Juvenile Growth Rate in Common Carp (Cyprinus carpio) Using Genotyping by Sequencing Article Snippet: Briefly, each sample (0.72 μg parental DNA/0.24 μg offspring DNA) was digested at 37°C for 60 min with Article Title: Mapping and Sequencing of a Significant Quantitative Trait Locus Affecting Resistance to Koi Herpesvirus in Common Carp Article Snippet: Briefly, template DNA was digested using the Article Title: Accuracy of Genomic Evaluations of Juvenile Growth Rate in Common Carp (Cyprinus carpio) Using Genotyping by Sequencing Article Snippet: Briefly, each sample (0.72 μg parental DNA/0.24 μg offspring DNA) was digested at 37°C for 60 min with Article Title: Mapping and Sequencing of a Significant Quantitative Trait Locus Affecting Resistance to Koi Herpesvirus in Common Carp Article Snippet: Briefly, template DNA was digested using the Transfection:Article Title: Long-term live imaging reveals cytosolic immune responses of host hepatocytes against Plasmodium infection and parasite escape mechanisms Article Snippet: This construct was used to target the GIMOANKA mother line using the ‘gene insertion/marker out’ (GIMO transfection) procedure. .. Construct pL1720 was made as follows: first the selectable marker (SM) cassette from pL1661 (containing both 5′hsp70 and 3′hsp70 sequences, tgdhfr/ts SM, and 230p targeting regions) was removed by digesting the plasmid with Sna BI (Roche, 10997480001) and Ligation:Article Title: Mapping and Validation of the Major Sex-Determining Region in Nile Tilapia (Oreochromis niloticus L.) Using RAD Sequencing Article Snippet: Each sample (0.72 µg parental DNA/0.24 µg offspring DNA) was digested at 37°C for 40 minutes with Article Title: Accuracy of Genomic Evaluations of Juvenile Growth Rate in Common Carp (Cyprinus carpio) Using Genotyping by Sequencing Article Snippet: Briefly, each sample (0.72 μg parental DNA/0.24 μg offspring DNA) was digested at 37°C for 60 min with Article Title: Mapping the sex determination locus in the Atlantic halibut (Hippoglossus hippoglossus) using RAD sequencing Article Snippet: Each sample (1.5 μg parental DNA / 0.5 μg offspring DNA) was digested at 37°C for 30 minutes with Article Title: Gene-centromere mapping in meiotic gynogenetic European seabass Article Snippet: Briefly, a single restriction enzyme digestion/adapter ligation reaction was performed for each progeny sample, while triplicate reactions were made for both dam and sire DNA samples. .. Each sample (40 ng DNA) was digested at 37 °C for 30 min with 0.8 U Article Title: A novel sex-determining QTL in Nile tilapia (Oreochromis niloticus) Article Snippet: Each sample (0.1 μg DNA) was digested at 37°C for 40 minutes with Article Title: Accuracy of Genomic Evaluations of Juvenile Growth Rate in Common Carp (Cyprinus carpio) Using Genotyping by Sequencing Article Snippet: Briefly, each sample (0.72 μg parental DNA/0.24 μg offspring DNA) was digested at 37°C for 60 min with Generated:Article Title: Characterization of the Rat Oncostatin M Receptor Complex Which Resembles the Human, but Differs from the Murine Cytokine Receptor Article Snippet: The rOSMR and rLIFR amplicons were digested with Polymerase Chain Reaction:Article Title: Characterization of the Rat Oncostatin M Receptor Complex Which Resembles the Human, but Differs from the Murine Cytokine Receptor Article Snippet: Upon reverse transcription, the cDNA was used to amplify the complete coding sequence of each receptor using specific primers containing restriction sites flanking the start or stop codon and the PCR Extender System (5 PRIME). .. The rOSMR and rLIFR amplicons were digested with Article Title: Mapping and Validation of the Major Sex-Determining Region in Nile Tilapia (Oreochromis niloticus L.) Using RAD Sequencing Article Snippet: The RAD specific P1 and P2 paired-end adapters and library amplification PCR primer sequences used in this study are detailed in Baxter et al. . .. Each sample (0.72 µg parental DNA/0.24 µg offspring DNA) was digested at 37°C for 40 minutes with Article Title: Accuracy of Genomic Evaluations of Juvenile Growth Rate in Common Carp (Cyprinus carpio) Using Genotyping by Sequencing Article Snippet: The RAD-specific P1 and P2 paired-end adapters and library amplification PCR primer sequences used in this study are detailed in . .. Briefly, each sample (0.72 μg parental DNA/0.24 μg offspring DNA) was digested at 37°C for 60 min with Article Title: Mapping the sex determination locus in the Atlantic halibut (Hippoglossus hippoglossus) using RAD sequencing Article Snippet: The RAD-specific P1 and P2 paired-end adapters and library amplification PCR primer sequences used in this study are detailed in Baxter et al. [ ]. .. Each sample (1.5 μg parental DNA / 0.5 μg offspring DNA) was digested at 37°C for 30 minutes with Article Title: Partners in Time: EARLY BIRD Associates with ZEITLUPE and Regulates the Speed of the Arabidopsis Clock 1Partners in Time: EARLY BIRD Associates with ZEITLUPE and Regulates the Speed of the Arabidopsis Clock 1 [W]Partners in Time: EARLY BIRD Associates with ZEITLUPE and Regulates the Speed of the Arabidopsis Clock 1 [W] [OA] Article Snippet: Complementation of the ebi-1 mutation was obtained by using the Myc-tagged CaMV 35S:EBI coding sequence in pRT104 plasmid (as described below), the construct was digested with Article Title: Genome-wide signatures of local adaptation among seven stoneflies species along a nationwide latitudinal gradient in Japan Article Snippet: Briefly, 100 ng genomic DNA per sample was digested with restriction endonucleases, Article Title: Accuracy of Genomic Evaluations of Juvenile Growth Rate in Common Carp (Cyprinus carpio) Using Genotyping by Sequencing Article Snippet: The RAD-specific P1 and P2 paired-end adapters and library amplification PCR primer sequences used in this study are detailed in . .. Briefly, each sample (0.72 μg parental DNA/0.24 μg offspring DNA) was digested at 37°C for 60 min with DNA Extraction:Article Title: Mapping and Validation of the Major Sex-Determining Region in Nile Tilapia (Oreochromis niloticus L.) Using RAD Sequencing Article Snippet: RAD Library Preparation and Sequencing DNA was extracted from fin tissue of the fish using the REALPure genomic DNA extraction kit (Durviz S.L.) and treated with RNase to remove residual RNA from the sample. .. Each sample (0.72 µg parental DNA/0.24 µg offspring DNA) was digested at 37°C for 40 minutes with Article Title: Accuracy of Genomic Evaluations of Juvenile Growth Rate in Common Carp (Cyprinus carpio) Using Genotyping by Sequencing Article Snippet: RAD Library Preparation and Sequencing Genomic DNA was extracted from fin samples using the REALPure genomic DNA extraction kit (Durviz S.L.) and treated with RNase. .. Briefly, each sample (0.72 μg parental DNA/0.24 μg offspring DNA) was digested at 37°C for 60 min with Article Title: Mapping the sex determination locus in the Atlantic halibut (Hippoglossus hippoglossus) using RAD sequencing Article Snippet: RAD library preparation and sequencing DNA was extracted from blood samples of the fish using the REALPure genomic DNA extraction kit (Durviz S.L.) and treated with RNase to remove residual RNA from the sample. .. Each sample (1.5 μg parental DNA / 0.5 μg offspring DNA) was digested at 37°C for 30 minutes with Article Title: Genome-wide signatures of local adaptation among seven stoneflies species along a nationwide latitudinal gradient in Japan Article Snippet: This DNA extraction method involved the use of the whole body without any destruction of the specimen, thus reducing the probability of contamination (gut content contamination). ddRAD libraries were constructed as previously described [ ] with slight modifications. .. Briefly, 100 ng genomic DNA per sample was digested with restriction endonucleases, Article Title: Accuracy of Genomic Evaluations of Juvenile Growth Rate in Common Carp (Cyprinus carpio) Using Genotyping by Sequencing Article Snippet: Genomic DNA was extracted from fin samples using the REALPure genomic DNA extraction kit (Durviz S.L.) and treated with RNase. .. Briefly, each sample (0.72 μg parental DNA/0.24 μg offspring DNA) was digested at 37°C for 60 min with Marker:Article Title: Long-term live imaging reveals cytosolic immune responses of host hepatocytes against Plasmodium infection and parasite escape mechanisms Article Snippet: .. Construct pL1720 was made as follows: first the selectable marker (SM) cassette from pL1661 (containing both 5′hsp70 and 3′hsp70 sequences, tgdhfr/ts SM, and 230p targeting regions) was removed by digesting the plasmid with Sna BI (Roche, 10997480001) and Mutagenesis:Article Title: Partners in Time: EARLY BIRD Associates with ZEITLUPE and Regulates the Speed of the Arabidopsis Clock 1Partners in Time: EARLY BIRD Associates with ZEITLUPE and Regulates the Speed of the Arabidopsis Clock 1 [W]Partners in Time: EARLY BIRD Associates with ZEITLUPE and Regulates the Speed of the Arabidopsis Clock 1 [W] [OA] Article Snippet: .. Complementation of the ebi-1 mutation was obtained by using the Myc-tagged CaMV 35S:EBI coding sequence in pRT104 plasmid (as described below), the construct was digested with Isolation:Article Title: Partners in Time: EARLY BIRD Associates with ZEITLUPE and Regulates the Speed of the Arabidopsis Clock 1Partners in Time: EARLY BIRD Associates with ZEITLUPE and Regulates the Speed of the Arabidopsis Clock 1 [W]Partners in Time: EARLY BIRD Associates with ZEITLUPE and Regulates the Speed of the Arabidopsis Clock 1 [W] [OA] Article Snippet: ebi-1 was isolated from a screen of EMS-mutagenized M2 Arabidopsis ( Arabidopsis thaliana ) Ws-2 plants ( ) by its early phase of CAB2 :: LUC+ expression in DD. .. Complementation of the ebi-1 mutation was obtained by using the Myc-tagged CaMV 35S:EBI coding sequence in pRT104 plasmid (as described below), the construct was digested with Article Title: Rapid SNP Discovery and Genetic Mapping Using Sequenced RAD Markers Article Snippet: .. Isolation of RAD markers for Illumina sequencing Genomic DNA (0.1–1 µg; from either individual or pooled samples) was digested for 15 min at 37°C in a 50 µL reaction with 20 units (U) of EcoR I or Purification:Article Title: A novel sex-determining QTL in Nile tilapia (Oreochromis niloticus) Article Snippet: Each sample (0.1 μg DNA) was digested at 37°C for 40 minutes with Article Title: Genome-wide signatures of local adaptation among seven stoneflies species along a nationwide latitudinal gradient in Japan Article Snippet: Briefly, 100 ng genomic DNA per sample was digested with restriction endonucleases, Sequencing:Article Title: Characterization of the Rat Oncostatin M Receptor Complex Which Resembles the Human, but Differs from the Murine Cytokine Receptor Article Snippet: Upon reverse transcription, the cDNA was used to amplify the complete coding sequence of each receptor using specific primers containing restriction sites flanking the start or stop codon and the PCR Extender System (5 PRIME). .. The rOSMR and rLIFR amplicons were digested with Article Title: Mapping the sex determination locus in the hāpuku (Polyprion oxygeneios) using ddRAD sequencing Article Snippet: .. ddRAD library preparation and sequencing Following a modified version of the protocol described by Peterson et al. [ ], two ddRAD libraries were prepared using different pairs of high fidelity restriction enzymes (REs) (New England Biolabs, UK; NEB); one using Article Title: Mapping and Validation of the Major Sex-Determining Region in Nile Tilapia (Oreochromis niloticus L.) Using RAD Sequencing Article Snippet: Paragraph title: RAD Library Preparation and Sequencing ... Each sample (0.72 µg parental DNA/0.24 µg offspring DNA) was digested at 37°C for 40 minutes with Article Title: Accuracy of Genomic Evaluations of Juvenile Growth Rate in Common Carp (Cyprinus carpio) Using Genotyping by Sequencing Article Snippet: Paragraph title: RAD Library Preparation and Sequencing ... Briefly, each sample (0.72 μg parental DNA/0.24 μg offspring DNA) was digested at 37°C for 60 min with Article Title: Mapping the sex determination locus in the Atlantic halibut (Hippoglossus hippoglossus) using RAD sequencing Article Snippet: Paragraph title: RAD library preparation and sequencing ... Each sample (1.5 μg parental DNA / 0.5 μg offspring DNA) was digested at 37°C for 30 minutes with Article Title: Partners in Time: EARLY BIRD Associates with ZEITLUPE and Regulates the Speed of the Arabidopsis Clock 1Partners in Time: EARLY BIRD Associates with ZEITLUPE and Regulates the Speed of the Arabidopsis Clock 1 [W]Partners in Time: EARLY BIRD Associates with ZEITLUPE and Regulates the Speed of the Arabidopsis Clock 1 [W] [OA] Article Snippet: .. Complementation of the ebi-1 mutation was obtained by using the Myc-tagged CaMV 35S:EBI coding sequence in pRT104 plasmid (as described below), the construct was digested with Article Title: Mapping and Sequencing of a Significant Quantitative Trait Locus Affecting Resistance to Koi Herpesvirus in Common Carp Article Snippet: Paragraph title: Library preparation and sequencing ... Briefly, template DNA was digested using the Article Title: Gene-centromere mapping in meiotic gynogenetic European seabass Article Snippet: Paragraph title: ddRAD library preparation and sequencing ... Each sample (40 ng DNA) was digested at 37 °C for 30 min with 0.8 U Article Title: A novel sex-determining QTL in Nile tilapia (Oreochromis niloticus) Article Snippet: Paragraph title: ddRAD library preparation and sequencing ... Each sample (0.1 μg DNA) was digested at 37°C for 40 minutes with Article Title: Genome-wide signatures of local adaptation among seven stoneflies species along a nationwide latitudinal gradient in Japan Article Snippet: Paragraph title: ddRAD library preparation and sequencing ... Briefly, 100 ng genomic DNA per sample was digested with restriction endonucleases, Article Title: Rapid SNP Discovery and Genetic Mapping Using Sequenced RAD Markers Article Snippet: .. Isolation of RAD markers for Illumina sequencing Genomic DNA (0.1–1 µg; from either individual or pooled samples) was digested for 15 min at 37°C in a 50 µL reaction with 20 units (U) of EcoR I or Article Title: Low recombination rates in sexual species and sex–asex transitions Article Snippet: Paragraph title: (iii) RAD sequencing ... Genomic DNA was digested with Lysis:Article Title: Genome-wide signatures of local adaptation among seven stoneflies species along a nationwide latitudinal gradient in Japan Article Snippet: The whole body of the specimen was fully submerged in lysis buffer (Qiagen GmbH, Hilden, Germany) and incubated at 56 °C overnight. .. Briefly, 100 ng genomic DNA per sample was digested with restriction endonucleases, Plasmid Preparation:Article Title: Characterization of the Rat Oncostatin M Receptor Complex Which Resembles the Human, but Differs from the Murine Cytokine Receptor Article Snippet: The rOSMR and rLIFR amplicons were digested with Article Title: Long-term live imaging reveals cytosolic immune responses of host hepatocytes against Plasmodium infection and parasite escape mechanisms Article Snippet: .. Construct pL1720 was made as follows: first the selectable marker (SM) cassette from pL1661 (containing both 5′hsp70 and 3′hsp70 sequences, tgdhfr/ts SM, and 230p targeting regions) was removed by digesting the plasmid with Sna BI (Roche, 10997480001) and Article Title: Partners in Time: EARLY BIRD Associates with ZEITLUPE and Regulates the Speed of the Arabidopsis Clock 1Partners in Time: EARLY BIRD Associates with ZEITLUPE and Regulates the Speed of the Arabidopsis Clock 1 [W]Partners in Time: EARLY BIRD Associates with ZEITLUPE and Regulates the Speed of the Arabidopsis Clock 1 [W] [OA] Article Snippet: .. Complementation of the ebi-1 mutation was obtained by using the Myc-tagged CaMV 35S:EBI coding sequence in pRT104 plasmid (as described below), the construct was digested with Multiplex Assay:Article Title: Mapping and Validation of the Major Sex-Determining Region in Nile Tilapia (Oreochromis niloticus L.) Using RAD Sequencing Article Snippet: Each sample (0.72 µg parental DNA/0.24 µg offspring DNA) was digested at 37°C for 40 minutes with Article Title: Accuracy of Genomic Evaluations of Juvenile Growth Rate in Common Carp (Cyprinus carpio) Using Genotyping by Sequencing Article Snippet: Briefly, each sample (0.72 μg parental DNA/0.24 μg offspring DNA) was digested at 37°C for 60 min with Article Title: Mapping the sex determination locus in the Atlantic halibut (Hippoglossus hippoglossus) using RAD sequencing Article Snippet: Each sample (1.5 μg parental DNA / 0.5 μg offspring DNA) was digested at 37°C for 30 minutes with Article Title: Accuracy of Genomic Evaluations of Juvenile Growth Rate in Common Carp (Cyprinus carpio) Using Genotyping by Sequencing Article Snippet: Briefly, each sample (0.72 μg parental DNA/0.24 μg offspring DNA) was digested at 37°C for 60 min with Selection:Article Title: Characterization of the Rat Oncostatin M Receptor Complex Which Resembles the Human, but Differs from the Murine Cytokine Receptor Article Snippet: The rOSMR and rLIFR amplicons were digested with Article Title: Accuracy of Genomic Evaluations of Juvenile Growth Rate in Common Carp (Cyprinus carpio) Using Genotyping by Sequencing Article Snippet: Briefly, each sample (0.72 μg parental DNA/0.24 μg offspring DNA) was digested at 37°C for 60 min with Article Title: Gene-centromere mapping in meiotic gynogenetic European seabass Article Snippet: In silico estimation from the seabass genome predicted 52,230 ddRAD fragments with paired SbfI-SphI restriction site overhangs, while after the size selection applied in the present study (c. 320 bp −590 bp excluding adaptors) only 3603 fragments were predicted to be available. .. Each sample (40 ng DNA) was digested at 37 °C for 30 min with 0.8 U Article Title: Accuracy of Genomic Evaluations of Juvenile Growth Rate in Common Carp (Cyprinus carpio) Using Genotyping by Sequencing Article Snippet: Briefly, each sample (0.72 μg parental DNA/0.24 μg offspring DNA) was digested at 37°C for 60 min with Agarose Gel Electrophoresis:Article Title: Mapping and Validation of the Major Sex-Determining Region in Nile Tilapia (Oreochromis niloticus L.) Using RAD Sequencing Article Snippet: Each sample was quantified by spectrophotometry (Nanodrop) and quality assessed by agarose gel electrophoresis, and was finally diluted to a concentration of 50 ng/µL in 5 mmol/L Tris, pH 8.5. .. Each sample (0.72 µg parental DNA/0.24 µg offspring DNA) was digested at 37°C for 40 minutes with Article Title: Accuracy of Genomic Evaluations of Juvenile Growth Rate in Common Carp (Cyprinus carpio) Using Genotyping by Sequencing Article Snippet: Each sample was quantified by spectrophotometry (Nanodrop), and its quality was assessed by agarose gel electrophoresis, before being diluted to a concentration of 20 ng/μL [measured by Qubit Fluorometer (Invitrogen)] in 5 mmol/L Tris, pH 8.5. .. Briefly, each sample (0.72 μg parental DNA/0.24 μg offspring DNA) was digested at 37°C for 60 min with Article Title: Mapping the sex determination locus in the Atlantic halibut (Hippoglossus hippoglossus) using RAD sequencing Article Snippet: Each sample was quantified by spectrophotometry (Nanodrop) and quality assessed by agarose gel electrophoresis, and was finally diluted to a concentration of 50 ng/μL in 5 mmol/L Tris, pH 8.5. .. Each sample (1.5 μg parental DNA / 0.5 μg offspring DNA) was digested at 37°C for 30 minutes with Article Title: Accuracy of Genomic Evaluations of Juvenile Growth Rate in Common Carp (Cyprinus carpio) Using Genotyping by Sequencing Article Snippet: Each sample was quantified by spectrophotometry (Nanodrop), and its quality was assessed by agarose gel electrophoresis, before being diluted to a concentration of 20 ng/μL [measured by Qubit Fluorometer (Invitrogen)] in 5 mmol/L Tris, pH 8.5. .. Briefly, each sample (0.72 μg parental DNA/0.24 μg offspring DNA) was digested at 37°C for 60 min with Article Title: Low recombination rates in sexual species and sex–asex transitions Article Snippet: Genomic DNA was digested with Transgenic Assay:Article Title: Partners in Time: EARLY BIRD Associates with ZEITLUPE and Regulates the Speed of the Arabidopsis Clock 1Partners in Time: EARLY BIRD Associates with ZEITLUPE and Regulates the Speed of the Arabidopsis Clock 1 [W]Partners in Time: EARLY BIRD Associates with ZEITLUPE and Regulates the Speed of the Arabidopsis Clock 1 [W] [OA] Article Snippet: Paragraph title: Plant Material and Transgenic Lines ... Complementation of the ebi-1 mutation was obtained by using the Myc-tagged CaMV 35S:EBI coding sequence in pRT104 plasmid (as described below), the construct was digested with Spectrophotometry:Article Title: Mapping and Validation of the Major Sex-Determining Region in Nile Tilapia (Oreochromis niloticus L.) Using RAD Sequencing Article Snippet: Each sample was quantified by spectrophotometry (Nanodrop) and quality assessed by agarose gel electrophoresis, and was finally diluted to a concentration of 50 ng/µL in 5 mmol/L Tris, pH 8.5. .. Each sample (0.72 µg parental DNA/0.24 µg offspring DNA) was digested at 37°C for 40 minutes with Article Title: Accuracy of Genomic Evaluations of Juvenile Growth Rate in Common Carp (Cyprinus carpio) Using Genotyping by Sequencing Article Snippet: Each sample was quantified by spectrophotometry (Nanodrop), and its quality was assessed by agarose gel electrophoresis, before being diluted to a concentration of 20 ng/μL [measured by Qubit Fluorometer (Invitrogen)] in 5 mmol/L Tris, pH 8.5. .. Briefly, each sample (0.72 μg parental DNA/0.24 μg offspring DNA) was digested at 37°C for 60 min with Article Title: Mapping the sex determination locus in the Atlantic halibut (Hippoglossus hippoglossus) using RAD sequencing Article Snippet: Each sample was quantified by spectrophotometry (Nanodrop) and quality assessed by agarose gel electrophoresis, and was finally diluted to a concentration of 50 ng/μL in 5 mmol/L Tris, pH 8.5. .. Each sample (1.5 μg parental DNA / 0.5 μg offspring DNA) was digested at 37°C for 30 minutes with Article Title: Accuracy of Genomic Evaluations of Juvenile Growth Rate in Common Carp (Cyprinus carpio) Using Genotyping by Sequencing Article Snippet: Each sample was quantified by spectrophotometry (Nanodrop), and its quality was assessed by agarose gel electrophoresis, before being diluted to a concentration of 20 ng/μL [measured by Qubit Fluorometer (Invitrogen)] in 5 mmol/L Tris, pH 8.5. .. Briefly, each sample (0.72 μg parental DNA/0.24 μg offspring DNA) was digested at 37°C for 60 min with Concentration Assay:Article Title: Mapping and Validation of the Major Sex-Determining Region in Nile Tilapia (Oreochromis niloticus L.) Using RAD Sequencing Article Snippet: .. Each sample (0.72 µg parental DNA/0.24 µg offspring DNA) was digested at 37°C for 40 minutes with Article Title: Accuracy of Genomic Evaluations of Juvenile Growth Rate in Common Carp (Cyprinus carpio) Using Genotyping by Sequencing Article Snippet: Each sample was quantified by spectrophotometry (Nanodrop), and its quality was assessed by agarose gel electrophoresis, before being diluted to a concentration of 20 ng/μL [measured by Qubit Fluorometer (Invitrogen)] in 5 mmol/L Tris, pH 8.5. .. Briefly, each sample (0.72 μg parental DNA/0.24 μg offspring DNA) was digested at 37°C for 60 min with Article Title: Mapping the sex determination locus in the Atlantic halibut (Hippoglossus hippoglossus) using RAD sequencing Article Snippet: .. Each sample (1.5 μg parental DNA / 0.5 μg offspring DNA) was digested at 37°C for 30 minutes with Article Title: Rapid SNP Discovery and Genetic Mapping Using Sequenced RAD Markers Article Snippet: Isolation of RAD markers for Illumina sequencing Genomic DNA (0.1–1 µg; from either individual or pooled samples) was digested for 15 min at 37°C in a 50 µL reaction with 20 units (U) of EcoR I or Article Title: Accuracy of Genomic Evaluations of Juvenile Growth Rate in Common Carp (Cyprinus carpio) Using Genotyping by Sequencing Article Snippet: Each sample was quantified by spectrophotometry (Nanodrop), and its quality was assessed by agarose gel electrophoresis, before being diluted to a concentration of 20 ng/μL [measured by Qubit Fluorometer (Invitrogen)] in 5 mmol/L Tris, pH 8.5. .. Briefly, each sample (0.72 μg parental DNA/0.24 μg offspring DNA) was digested at 37°C for 60 min with High Throughput Screening Assay:Article Title: Mapping and Sequencing of a Significant Quantitative Trait Locus Affecting Resistance to Koi Herpesvirus in Common Carp Article Snippet: Briefly, template DNA was digested using the Article Title: Mapping and Sequencing of a Significant Quantitative Trait Locus Affecting Resistance to Koi Herpesvirus in Common Carp Article Snippet: Briefly, template DNA was digested using the Gel Extraction:Article Title: Genome-wide signatures of local adaptation among seven stoneflies species along a nationwide latitudinal gradient in Japan Article Snippet: Briefly, 100 ng genomic DNA per sample was digested with restriction endonucleases, Fluorescence In Situ Hybridization:Article Title: Mapping and Validation of the Major Sex-Determining Region in Nile Tilapia (Oreochromis niloticus L.) Using RAD Sequencing Article Snippet: RAD Library Preparation and Sequencing DNA was extracted from fin tissue of the fish using the REALPure genomic DNA extraction kit (Durviz S.L.) and treated with RNase to remove residual RNA from the sample. .. Each sample (0.72 µg parental DNA/0.24 µg offspring DNA) was digested at 37°C for 40 minutes with Article Title: Mapping the sex determination locus in the Atlantic halibut (Hippoglossus hippoglossus) using RAD sequencing Article Snippet: RAD library preparation and sequencing DNA was extracted from blood samples of the fish using the REALPure genomic DNA extraction kit (Durviz S.L.) and treated with RNase to remove residual RNA from the sample. .. Each sample (1.5 μg parental DNA / 0.5 μg offspring DNA) was digested at 37°C for 30 minutes with |
