cviaii  (New England Biolabs)


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    Name:
    CviAII
    Description:
    CviAII 1 000 units
    Catalog Number:
    R0640L
    Price:
    269
    Category:
    Restriction Enzymes
    Size:
    1 000 units
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    New England Biolabs cviaii
    CviAII
    CviAII 1 000 units
    https://www.bioz.com/result/cviaii/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cviaii - by Bioz Stars, 2021-06
    95/100 stars

    Images

    1) Product Images from "Effect of valproic acid on mitochondrial epigenetics"

    Article Title: Effect of valproic acid on mitochondrial epigenetics

    Journal: European journal of pharmacology

    doi: 10.1016/j.ejphar.2012.06.019

    Representation of the selected mouse mitochondrial DNA regions analyzed with the beta-glu-5hmC-sensitive restriction enzymes CviAII and CviQI for 5hmC modifications. The presentation is based on the Mus musculus mitochondrion, complete genome 16299 residue.
    Figure Legend Snippet: Representation of the selected mouse mitochondrial DNA regions analyzed with the beta-glu-5hmC-sensitive restriction enzymes CviAII and CviQI for 5hmC modifications. The presentation is based on the Mus musculus mitochondrion, complete genome 16299 residue.

    Techniques Used:

    2) Product Images from "Dynamics of TRF1 organizing a single human telomere"

    Article Title: Dynamics of TRF1 organizing a single human telomere

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkaa1222

    Preparation of single human telomeres and length measurements using force–extension assays. ( A ) Scheme of single telomere preparation. The genome of K562 cells underwent digestion by four restriction enzymes, BfaI, CviAII, MseI, and NdeI. Genome digestion generates AT or TA overhangs in the residual subtelomere. Digoxigenin-modified dUTP (Orange sphere) fills in the AT or TA overhangs via Klenow reaction. Biotin-modified (5′-TAACCC-3′) 3 oligos (Green sphere) recognize the telomeric ssDNA overhang by base pairing. Digoxigenin and biotin enable a single human telomere to interact with glass slide treated by anti-digoxigenin antibody and streptavidin-coated beads, respectively. ( B ) Telomere length measurements by force–extension assays. In a reaction chamber of single-molecule magnetic tweezers containing a buffer of 20 mM of HEPES (pH 7.5), 1 mM of EDTA, 100 mM of NaCl and 0.0063% Tween-20 at 23°C, two surfaces of a glass slide and a microsphere immobilized a single human telomere via digoxigenin–antibody and biotin–streptavidin affinity interactions (cartoon). Using single-molecule force spectroscopy, four typical force–extension curves of single human telomeres show distinct length responses to forces ranged between 0 and 17 pN with a force loading rate of ±4 pN/s. ( C ) Length distribution of single human telomeres from K562 leukemia cells measured by single-molecule magnetic tweezers. The distribution shows a length with 2.5 ± 0.9 kb (mean ± SD, n = 1975). The minimum and maximum lengths are 0.3 and 7.4 kb, respectively. ( D ) Terminal restriction fragment (TRF) analysis using CviAII/NdeI/MseI/BfaI enzymes. Marker sizes are noted in the M lane. Lane 1 and lane 2 contain 2 μg and 4 μg DNA, respectively. The estimated TL is 2.7 ± 2.3 kb based on lane 2 using the software of TeloTool ( 39 ).
    Figure Legend Snippet: Preparation of single human telomeres and length measurements using force–extension assays. ( A ) Scheme of single telomere preparation. The genome of K562 cells underwent digestion by four restriction enzymes, BfaI, CviAII, MseI, and NdeI. Genome digestion generates AT or TA overhangs in the residual subtelomere. Digoxigenin-modified dUTP (Orange sphere) fills in the AT or TA overhangs via Klenow reaction. Biotin-modified (5′-TAACCC-3′) 3 oligos (Green sphere) recognize the telomeric ssDNA overhang by base pairing. Digoxigenin and biotin enable a single human telomere to interact with glass slide treated by anti-digoxigenin antibody and streptavidin-coated beads, respectively. ( B ) Telomere length measurements by force–extension assays. In a reaction chamber of single-molecule magnetic tweezers containing a buffer of 20 mM of HEPES (pH 7.5), 1 mM of EDTA, 100 mM of NaCl and 0.0063% Tween-20 at 23°C, two surfaces of a glass slide and a microsphere immobilized a single human telomere via digoxigenin–antibody and biotin–streptavidin affinity interactions (cartoon). Using single-molecule force spectroscopy, four typical force–extension curves of single human telomeres show distinct length responses to forces ranged between 0 and 17 pN with a force loading rate of ±4 pN/s. ( C ) Length distribution of single human telomeres from K562 leukemia cells measured by single-molecule magnetic tweezers. The distribution shows a length with 2.5 ± 0.9 kb (mean ± SD, n = 1975). The minimum and maximum lengths are 0.3 and 7.4 kb, respectively. ( D ) Terminal restriction fragment (TRF) analysis using CviAII/NdeI/MseI/BfaI enzymes. Marker sizes are noted in the M lane. Lane 1 and lane 2 contain 2 μg and 4 μg DNA, respectively. The estimated TL is 2.7 ± 2.3 kb based on lane 2 using the software of TeloTool ( 39 ).

    Techniques Used: Modification, Spectroscopy, Marker, Software

    Related Articles

    Sequencing:

    Article Title: Effect of valproic acid on mitochondrial epigenetics
    Article Snippet: For measurement of 5mC content, we used the corresponding Methylated DNA Quantification Kit from Epigentek. .. The sequence-specific 5hmC content in selected mtDNA regions was measured by qPCR applied to samples after a glucosyltransferase step followed by an enzymatic restriction digest with two enzymes, CviAII (CATG recognition site) and CviQI (GTAC recognition site) (New England Biolabs; Ipswich, MA) ( ). .. First, DNA samples are glucosylated with phage β-glucosyltransferase (T4-BGT; New England Biolabs, Ipswich, MA), which specifically transfers the glucose moiety of uridine diphosphoglucose (UDP-Glc) to the 5hmC residues but not to the 5mC residues.

    Real-time Polymerase Chain Reaction:

    Article Title: Effect of valproic acid on mitochondrial epigenetics
    Article Snippet: For measurement of 5mC content, we used the corresponding Methylated DNA Quantification Kit from Epigentek. .. The sequence-specific 5hmC content in selected mtDNA regions was measured by qPCR applied to samples after a glucosyltransferase step followed by an enzymatic restriction digest with two enzymes, CviAII (CATG recognition site) and CviQI (GTAC recognition site) (New England Biolabs; Ipswich, MA) ( ). .. First, DNA samples are glucosylated with phage β-glucosyltransferase (T4-BGT; New England Biolabs, Ipswich, MA), which specifically transfers the glucose moiety of uridine diphosphoglucose (UDP-Glc) to the 5hmC residues but not to the 5mC residues.

    Modification:

    Article Title: Dynamics of TRF1 organizing a single human telomere
    Article Snippet: DNA samples without a smear in the agarose gel went for restriction enzyme digestion. .. We first digested 20 μg of K562 DNA with 10 U of CviAII in 50 μl of 1× CutSmart® buffer (NEB, USA) at 25°C for 12 h. We next supplemented the reaction with three more enzymes (BfaI/MseI/NdeI, 10 U of each) and continued the digestion at 37°C for another 12 h. Enzymes were heat-inactivated at 80°C for 20 min. We then filled the 5′ overhangs (TA or AT) of K562 DNA fragments using 5 U of Klenow fragment (3′-5′ exo-) with 1:1 ratio mixture of dATP and digoxigenin-dUTP (Cat#: 11093088910, Roche, Switzerland) at 37°C for 12 h. Klenow reaction thus modified the proximal ends of K562 telomeres with digoxigenin-dUTP for later affinity interactions. .. We commercially synthesized biotinylated DNA oligo of biotin-(5′-TAACCC-3′)3.

    Article Title: Effect of aging on 5-hydroxymethylcytosine in brain mitochondria
    Article Snippet: The 5hmC modifications of mtDNA were quantified using a glucosyltransferase assay that involves an enzymatic restriction digest combined with qPCR; the principle of the method described and visualized in . .. We have modified this assay by selecting a different set of restriction enzymes, i.e., CviAII (CATG recognition site) and CviQI (GTAC recognition site) (New England Biolabs, Ipswich, MA, USA). .. Both enzymes are insensitive to cytosine methylation and hydroxymethylation (CviAII is only partially, about 25% sensitive to 5hmC).

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    New England Biolabs cviaii
    Representation of the selected mouse mitochondrial DNA regions analyzed with the beta-glu-5hmC-sensitive restriction enzymes <t>CviAII</t> and <t>CviQI</t> for 5hmC modifications. The presentation is based on the Mus musculus mitochondrion, complete genome 16299 residue.
    Cviaii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cviaii/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
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    Representation of the selected mouse mitochondrial DNA regions analyzed with the beta-glu-5hmC-sensitive restriction enzymes CviAII and CviQI for 5hmC modifications. The presentation is based on the Mus musculus mitochondrion, complete genome 16299 residue.

    Journal: European journal of pharmacology

    Article Title: Effect of valproic acid on mitochondrial epigenetics

    doi: 10.1016/j.ejphar.2012.06.019

    Figure Lengend Snippet: Representation of the selected mouse mitochondrial DNA regions analyzed with the beta-glu-5hmC-sensitive restriction enzymes CviAII and CviQI for 5hmC modifications. The presentation is based on the Mus musculus mitochondrion, complete genome 16299 residue.

    Article Snippet: The sequence-specific 5hmC content in selected mtDNA regions was measured by qPCR applied to samples after a glucosyltransferase step followed by an enzymatic restriction digest with two enzymes, CviAII (CATG recognition site) and CviQI (GTAC recognition site) (New England Biolabs; Ipswich, MA) ( ).

    Techniques:

    Preparation of single human telomeres and length measurements using force–extension assays. ( A ) Scheme of single telomere preparation. The genome of K562 cells underwent digestion by four restriction enzymes, BfaI, CviAII, MseI, and NdeI. Genome digestion generates AT or TA overhangs in the residual subtelomere. Digoxigenin-modified dUTP (Orange sphere) fills in the AT or TA overhangs via Klenow reaction. Biotin-modified (5′-TAACCC-3′) 3 oligos (Green sphere) recognize the telomeric ssDNA overhang by base pairing. Digoxigenin and biotin enable a single human telomere to interact with glass slide treated by anti-digoxigenin antibody and streptavidin-coated beads, respectively. ( B ) Telomere length measurements by force–extension assays. In a reaction chamber of single-molecule magnetic tweezers containing a buffer of 20 mM of HEPES (pH 7.5), 1 mM of EDTA, 100 mM of NaCl and 0.0063% Tween-20 at 23°C, two surfaces of a glass slide and a microsphere immobilized a single human telomere via digoxigenin–antibody and biotin–streptavidin affinity interactions (cartoon). Using single-molecule force spectroscopy, four typical force–extension curves of single human telomeres show distinct length responses to forces ranged between 0 and 17 pN with a force loading rate of ±4 pN/s. ( C ) Length distribution of single human telomeres from K562 leukemia cells measured by single-molecule magnetic tweezers. The distribution shows a length with 2.5 ± 0.9 kb (mean ± SD, n = 1975). The minimum and maximum lengths are 0.3 and 7.4 kb, respectively. ( D ) Terminal restriction fragment (TRF) analysis using CviAII/NdeI/MseI/BfaI enzymes. Marker sizes are noted in the M lane. Lane 1 and lane 2 contain 2 μg and 4 μg DNA, respectively. The estimated TL is 2.7 ± 2.3 kb based on lane 2 using the software of TeloTool ( 39 ).

    Journal: Nucleic Acids Research

    Article Title: Dynamics of TRF1 organizing a single human telomere

    doi: 10.1093/nar/gkaa1222

    Figure Lengend Snippet: Preparation of single human telomeres and length measurements using force–extension assays. ( A ) Scheme of single telomere preparation. The genome of K562 cells underwent digestion by four restriction enzymes, BfaI, CviAII, MseI, and NdeI. Genome digestion generates AT or TA overhangs in the residual subtelomere. Digoxigenin-modified dUTP (Orange sphere) fills in the AT or TA overhangs via Klenow reaction. Biotin-modified (5′-TAACCC-3′) 3 oligos (Green sphere) recognize the telomeric ssDNA overhang by base pairing. Digoxigenin and biotin enable a single human telomere to interact with glass slide treated by anti-digoxigenin antibody and streptavidin-coated beads, respectively. ( B ) Telomere length measurements by force–extension assays. In a reaction chamber of single-molecule magnetic tweezers containing a buffer of 20 mM of HEPES (pH 7.5), 1 mM of EDTA, 100 mM of NaCl and 0.0063% Tween-20 at 23°C, two surfaces of a glass slide and a microsphere immobilized a single human telomere via digoxigenin–antibody and biotin–streptavidin affinity interactions (cartoon). Using single-molecule force spectroscopy, four typical force–extension curves of single human telomeres show distinct length responses to forces ranged between 0 and 17 pN with a force loading rate of ±4 pN/s. ( C ) Length distribution of single human telomeres from K562 leukemia cells measured by single-molecule magnetic tweezers. The distribution shows a length with 2.5 ± 0.9 kb (mean ± SD, n = 1975). The minimum and maximum lengths are 0.3 and 7.4 kb, respectively. ( D ) Terminal restriction fragment (TRF) analysis using CviAII/NdeI/MseI/BfaI enzymes. Marker sizes are noted in the M lane. Lane 1 and lane 2 contain 2 μg and 4 μg DNA, respectively. The estimated TL is 2.7 ± 2.3 kb based on lane 2 using the software of TeloTool ( 39 ).

    Article Snippet: We first digested 20 μg of K562 DNA with 10 U of CviAII in 50 μl of 1× CutSmart® buffer (NEB, USA) at 25°C for 12 h. We next supplemented the reaction with three more enzymes (BfaI/MseI/NdeI, 10 U of each) and continued the digestion at 37°C for another 12 h. Enzymes were heat-inactivated at 80°C for 20 min. We then filled the 5′ overhangs (TA or AT) of K562 DNA fragments using 5 U of Klenow fragment (3′-5′ exo-) with 1:1 ratio mixture of dATP and digoxigenin-dUTP (Cat#: 11093088910, Roche, Switzerland) at 37°C for 12 h. Klenow reaction thus modified the proximal ends of K562 telomeres with digoxigenin-dUTP for later affinity interactions.

    Techniques: Modification, Spectroscopy, Marker, Software