cviaii restriction enzyme  (New England Biolabs)


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    Name:
    CviAII
    Description:
    CviAII 1 000 units
    Catalog Number:
    r0640l
    Price:
    269
    Size:
    1 000 units
    Category:
    Restriction Enzymes
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    Structured Review

    New England Biolabs cviaii restriction enzyme
    CviAII
    CviAII 1 000 units
    https://www.bioz.com/result/cviaii restriction enzyme/product/New England Biolabs
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cviaii restriction enzyme - by Bioz Stars, 2020-04
    90/100 stars

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    Related Articles

    Amplification:

    Article Title: Atypical Porcine Pestivirus: A Possible Cause of Congenital Tremor Type A-II in Newborn Piglets
    Article Snippet: Double-stranded DNA was purified by phenol/chloroform/isoamyl alcohol extraction and ethanol precipitation and digested with either MseI, Csp6I or CviAII restriction enzyme (New England Biolabs). .. Before PCR amplification, the fragments were purified with AMPure XP beads (Agencourt AMPure XP PCR, Beckman Coulter, Brea, CA, USA).

    Article Title: The RNA interactome of human telomerase RNA reveals a coding-independent role for a histone mRNA in telomere homeostasis
    Article Snippet: Oligonucleotide sequences for the ligation and amplification reactions were published in . .. Briefly, 50 ng of genomic DNA was ligated with TeSLA-T oligonucleotides and then digested with CviAII, BfaI, NdeI, and MseI restriction enzymes (New England Biolabs), followed by shrimp alkaline phosphatase (New England Biolabs) treatment.

    Article Title: Multiplex amplification enabled by selective circularization of large sets of genomic DNA fragments
    Article Snippet: Paragraph title: Circularization and amplification of 96 fragments ... Two combinations of restriction enzymes, FH : 10 U of Fsp I (Fermentas) and 10 U of HpyCH4 V (New England Biolabs); AC : 10 U of Acu I (New England Biolabs) and 10 U of CviA II (New England Biolabs), were added to two different aliquots of 10 μg genomic DNA and 0.5 μg BSA, in a total volume of 50 μl NEBuffer 4.

    Article Title: Genetic association study from North India to analyze association of CYP19A1 and CYP17A1 with polycystic ovary syndrome
    Article Snippet: .. PCR amplification for rs2414096 (G > A) was done, using primers as given by Jin et al. [ ] CViAII (New England Biolabs) restriction enzyme digested PCR product when treated overnight at 37 °C. .. After digestion, a band of 189 bp for GG; 189-, 161-, and 28-bp band for GA; and 161- and 28-bp band for AA genotype were seen on 2.5% agarose gel.

    Article Title: Effect of aging on 5-hydroxymethylcytosine in brain mitochondria
    Article Snippet: We have modified this assay by selecting a different set of restriction enzymes, i.e., CviAII (CATG recognition site) and CviQI (GTAC recognition site) (New England Biolabs, Ipswich, MA, USA). .. The target DNA sequence (digested and glucosylated) is amplified and measured by qPCR.

    DNA Synthesis:

    Article Title: Atypical Porcine Pestivirus: A Possible Cause of Congenital Tremor Type A-II in Newborn Piglets
    Article Snippet: A second strand DNA synthesis was performed with 5 U of Klenow fragment (3′ to 5′ exo minus, New England Biolabs, Ipswich, MA, USA). .. Double-stranded DNA was purified by phenol/chloroform/isoamyl alcohol extraction and ethanol precipitation and digested with either MseI, Csp6I or CviAII restriction enzyme (New England Biolabs).

    Picogreen Assay:

    Article Title: Genetic Diversity and Population Structure of the USDA Sweetpotato (Ipomoea batatas) Germplasm Collections Using GBSpoly
    Article Snippet: A double-digest was performed using 1 μg of DNA in a total volume of 30 μl with 5 units of Cvi AII at 25°C for 3 h and then with 5 units of Tse I at 65°C for 3 h in NEB CutSmart buffer (New England Biolabs). .. The digested DNA samples were purified with AMPure XP magnetic beads (ThermoFisher), quantified using a picogreen assay and then diluted to a concentration of 10 ng/μl.

    Article Title: Genetic Diversity and Population Structure of the USDA Sweetpotato (Ipomoea batatas) Germplasm Collections Using GBSpoly
    Article Snippet: A double-digest was performed using 1 μg of DNA in a total volume of 30 μl with 5 units of Cvi AII at 25°C for 3 h and then with 5 units of Tse I at 65°C for 3 h in NEB CutSmart buffer (New England Biolabs). .. The digested DNA samples were purified with AMPure XP magnetic beads (ThermoFisher), quantified using a picogreen assay and then diluted to a concentration of 10 ng/μl.

    Real-time Polymerase Chain Reaction:

    Article Title: Effect of valproic acid on mitochondrial epigenetics
    Article Snippet: .. The sequence-specific 5hmC content in selected mtDNA regions was measured by qPCR applied to samples after a glucosyltransferase step followed by an enzymatic restriction digest with two enzymes, CviAII (CATG recognition site) and CviQI (GTAC recognition site) (New England Biolabs; Ipswich, MA) ( ). .. First, DNA samples are glucosylated with phage β-glucosyltransferase (T4-BGT; New England Biolabs, Ipswich, MA), which specifically transfers the glucose moiety of uridine diphosphoglucose (UDP-Glc) to the 5hmC residues but not to the 5mC residues.

    Article Title: Effect of aging on 5-hydroxymethylcytosine in brain mitochondria
    Article Snippet: The 5hmC modifications of mtDNA were quantified using a glucosyltransferase assay that involves an enzymatic restriction digest combined with qPCR; the principle of the method described and visualized in . .. We have modified this assay by selecting a different set of restriction enzymes, i.e., CviAII (CATG recognition site) and CviQI (GTAC recognition site) (New England Biolabs, Ipswich, MA, USA).

    TALENs:

    Article Title: microRNAs Establish Uniform Traits during the Architecture of Vertebrate Embryos
    Article Snippet: As previously described, TALENs were used to create miR-139 and miR-223 mutants and a multiplexed pool of CRISPR guide RNA pairs and Cas9 mRNA was used to simultaneously mutagenize all four miR-24 genes ( ; ). .. Mutagenesis was initially determined by restriction endonuclease assay using CviAII (NEB) for the miR-139 locus and T7 endonuclease I (NEB) for miR-24 and miR-223 genes.

    Modification:

    Article Title: Genetic Diversity and Population Structure of the USDA Sweetpotato (Ipomoea batatas) Germplasm Collections Using GBSpoly
    Article Snippet: A modified genotyping-by-sequencing (GBSpoly) protocol optimized for highly heterozygous and polyploid genomes was implemented ( ). .. A double-digest was performed using 1 μg of DNA in a total volume of 30 μl with 5 units of Cvi AII at 25°C for 3 h and then with 5 units of Tse I at 65°C for 3 h in NEB CutSmart buffer (New England Biolabs).

    Article Title: Genetic Diversity and Population Structure of the USDA Sweetpotato (Ipomoea batatas) Germplasm Collections Using GBSpoly
    Article Snippet: A modified genotyping-by-sequencing (GBSpoly) protocol optimized for highly heterozygous and polyploid genomes was implemented ( ). .. A double-digest was performed using 1 μg of DNA in a total volume of 30 μl with 5 units of Cvi AII at 25°C for 3 h and then with 5 units of Tse I at 65°C for 3 h in NEB CutSmart buffer (New England Biolabs).

    Article Title: Effect of valproic acid on mitochondrial epigenetics
    Article Snippet: The sequence-specific 5hmC content in selected mtDNA regions was measured by qPCR applied to samples after a glucosyltransferase step followed by an enzymatic restriction digest with two enzymes, CviAII (CATG recognition site) and CviQI (GTAC recognition site) (New England Biolabs; Ipswich, MA) ( ). .. Both restriction enzymes used in our assay are insensitive to cytosine methylation and hydroxymethylation (CviAII is only partially sensitive, about 25% to 5hmC) but are sensitive to beta-glu-5hmC modification.

    Article Title: Effect of aging on 5-hydroxymethylcytosine in brain mitochondria
    Article Snippet: .. We have modified this assay by selecting a different set of restriction enzymes, i.e., CviAII (CATG recognition site) and CviQI (GTAC recognition site) (New England Biolabs, Ipswich, MA, USA). .. Both enzymes are insensitive to cytosine methylation and hydroxymethylation (CviAII is only partially, about 25% sensitive to 5hmC).

    Ligation:

    Article Title: The RNA interactome of human telomerase RNA reveals a coding-independent role for a histone mRNA in telomere homeostasis
    Article Snippet: Oligonucleotide sequences for the ligation and amplification reactions were published in . .. Briefly, 50 ng of genomic DNA was ligated with TeSLA-T oligonucleotides and then digested with CviAII, BfaI, NdeI, and MseI restriction enzymes (New England Biolabs), followed by shrimp alkaline phosphatase (New England Biolabs) treatment.

    Article Title: CTCF-mediated enhancer-promoter interaction is a critical regulator of cell-to-cell variation of gene expression
    Article Snippet: Chromatin was subsequently digested with 20 Units CviQ I (NEB), and 20 Units CviA II (NEB) at 25°C for 20 minutes, then with 20 Units Bfa I (NEB) at 37°C for 20 minutes. .. The DNA ends were blunted and labeled with biotin by Klenow enzyme in the presence of dCTP, dGTP, dTTP, biotin-14-dATP, followed by ligation using T4 DNA ligase.

    Article Title: The Maintenance of Telomere Length in CD28+ T Cells During T Lymphocyte Stimulation
    Article Snippet: .. After ligation, the genomic DNA is digested by a series of restriction enzymes including CviAII, BfaI, NdeI, and MseI (New England Biolabs) to digest all DNA but not telomeres which do not have restriction site for these enzymes. .. The digested genomic DNA is next treated with Shrimp Alkaline Phosphatase (rSAP; New England Biolabs) to remove 5′ phosphate from each DNA fragment to improve the specificity of ligation between overhang adapters and genomic DNA fragments.

    Magnetic Beads:

    Article Title: Genetic Diversity and Population Structure of the USDA Sweetpotato (Ipomoea batatas) Germplasm Collections Using GBSpoly
    Article Snippet: A double-digest was performed using 1 μg of DNA in a total volume of 30 μl with 5 units of Cvi AII at 25°C for 3 h and then with 5 units of Tse I at 65°C for 3 h in NEB CutSmart buffer (New England Biolabs). .. The digested DNA samples were purified with AMPure XP magnetic beads (ThermoFisher), quantified using a picogreen assay and then diluted to a concentration of 10 ng/μl.

    Article Title: Genetic Diversity and Population Structure of the USDA Sweetpotato (Ipomoea batatas) Germplasm Collections Using GBSpoly
    Article Snippet: A double-digest was performed using 1 μg of DNA in a total volume of 30 μl with 5 units of Cvi AII at 25°C for 3 h and then with 5 units of Tse I at 65°C for 3 h in NEB CutSmart buffer (New England Biolabs). .. The digested DNA samples were purified with AMPure XP magnetic beads (ThermoFisher), quantified using a picogreen assay and then diluted to a concentration of 10 ng/μl.

    Sequencing:

    Article Title: Atypical Porcine Pestivirus: A Possible Cause of Congenital Tremor Type A-II in Newborn Piglets
    Article Snippet: Paragraph title: 2.3. VIDISCA-Next Generation Sequencing ... Double-stranded DNA was purified by phenol/chloroform/isoamyl alcohol extraction and ethanol precipitation and digested with either MseI, Csp6I or CviAII restriction enzyme (New England Biolabs).

    Article Title: Genetic Diversity and Population Structure of the USDA Sweetpotato (Ipomoea batatas) Germplasm Collections Using GBSpoly
    Article Snippet: A double-digest was performed using 1 μg of DNA in a total volume of 30 μl with 5 units of Cvi AII at 25°C for 3 h and then with 5 units of Tse I at 65°C for 3 h in NEB CutSmart buffer (New England Biolabs). .. The resulting fragments were ligated to barcoded adapters which were designed to contain an 8-bp buffer sequence positioned upstream of the variable length (6–9 bp) barcode sequence (multiplexed for 96 pooled samples).

    Article Title: Genetic Diversity and Population Structure of the USDA Sweetpotato (Ipomoea batatas) Germplasm Collections Using GBSpoly
    Article Snippet: A double-digest was performed using 1 μg of DNA in a total volume of 30 μl with 5 units of Cvi AII at 25°C for 3 h and then with 5 units of Tse I at 65°C for 3 h in NEB CutSmart buffer (New England Biolabs). .. The resulting fragments were ligated to barcoded adapters which were designed to contain an 8-bp buffer sequence positioned upstream of the variable length (6–9 bp) barcode sequence (multiplexed for 96 pooled samples).

    Article Title: Effect of valproic acid on mitochondrial epigenetics
    Article Snippet: .. The sequence-specific 5hmC content in selected mtDNA regions was measured by qPCR applied to samples after a glucosyltransferase step followed by an enzymatic restriction digest with two enzymes, CviAII (CATG recognition site) and CviQI (GTAC recognition site) (New England Biolabs; Ipswich, MA) ( ). .. First, DNA samples are glucosylated with phage β-glucosyltransferase (T4-BGT; New England Biolabs, Ipswich, MA), which specifically transfers the glucose moiety of uridine diphosphoglucose (UDP-Glc) to the 5hmC residues but not to the 5mC residues.

    Article Title: Effect of aging on 5-hydroxymethylcytosine in brain mitochondria
    Article Snippet: Paragraph title: 2.6. Sequence-specific mtDNA 5hmC assay ... We have modified this assay by selecting a different set of restriction enzymes, i.e., CviAII (CATG recognition site) and CviQI (GTAC recognition site) (New England Biolabs, Ipswich, MA, USA).

    Injection:

    Article Title: microRNAs Establish Uniform Traits during the Architecture of Vertebrate Embryos
    Article Snippet: Mutagenesis was initially determined by restriction endonuclease assay using CviAII (NEB) for the miR-139 locus and T7 endonuclease I (NEB) for miR-24 and miR-223 genes. .. Briefly, 50 ng of genomic DNA isolated with the DNeasy Blood and Tissue kit (Qiagen) from a clutch of 15–20 injected 24 hpf embryos was used to amplify a 200–400 bp region spanning the intended mutation site.

    Hi-C:

    Article Title: CTCF-mediated enhancer-promoter interaction is a critical regulator of cell-to-cell variation of gene expression
    Article Snippet: Paragraph title: 3e Hi-C assay ... Chromatin was subsequently digested with 20 Units CviQ I (NEB), and 20 Units CviA II (NEB) at 25°C for 20 minutes, then with 20 Units Bfa I (NEB) at 37°C for 20 minutes.

    DNA Extraction:

    Article Title: The Maintenance of Telomere Length in CD28+ T Cells During T Lymphocyte Stimulation
    Article Snippet: Paragraph title: DNA extraction and telomeric measurement assays ... After ligation, the genomic DNA is digested by a series of restriction enzymes including CviAII, BfaI, NdeI, and MseI (New England Biolabs) to digest all DNA but not telomeres which do not have restriction site for these enzymes.

    Methylation:

    Article Title: Effect of valproic acid on mitochondrial epigenetics
    Article Snippet: The sequence-specific 5hmC content in selected mtDNA regions was measured by qPCR applied to samples after a glucosyltransferase step followed by an enzymatic restriction digest with two enzymes, CviAII (CATG recognition site) and CviQI (GTAC recognition site) (New England Biolabs; Ipswich, MA) ( ). .. Both restriction enzymes used in our assay are insensitive to cytosine methylation and hydroxymethylation (CviAII is only partially sensitive, about 25% to 5hmC) but are sensitive to beta-glu-5hmC modification.

    Article Title: Effect of aging on 5-hydroxymethylcytosine in brain mitochondria
    Article Snippet: We have modified this assay by selecting a different set of restriction enzymes, i.e., CviAII (CATG recognition site) and CviQI (GTAC recognition site) (New England Biolabs, Ipswich, MA, USA). .. Both enzymes are insensitive to cytosine methylation and hydroxymethylation (CviAII is only partially, about 25% sensitive to 5hmC).

    Mutagenesis:

    Article Title: microRNAs Establish Uniform Traits during the Architecture of Vertebrate Embryos
    Article Snippet: .. Mutagenesis was initially determined by restriction endonuclease assay using CviAII (NEB) for the miR-139 locus and T7 endonuclease I (NEB) for miR-24 and miR-223 genes. .. Briefly, 50 ng of genomic DNA isolated with the DNeasy Blood and Tissue kit (Qiagen) from a clutch of 15–20 injected 24 hpf embryos was used to amplify a 200–400 bp region spanning the intended mutation site.

    Isolation:

    Article Title: Genetic Diversity and Population Structure of the USDA Sweetpotato (Ipomoea batatas) Germplasm Collections Using GBSpoly
    Article Snippet: A double-digest was performed using 1 μg of DNA in a total volume of 30 μl with 5 units of Cvi AII at 25°C for 3 h and then with 5 units of Tse I at 65°C for 3 h in NEB CutSmart buffer (New England Biolabs). .. A double-digest was performed using 1 μg of DNA in a total volume of 30 μl with 5 units of Cvi AII at 25°C for 3 h and then with 5 units of Tse I at 65°C for 3 h in NEB CutSmart buffer (New England Biolabs).

    Article Title: Genetic Diversity and Population Structure of the USDA Sweetpotato (Ipomoea batatas) Germplasm Collections Using GBSpoly
    Article Snippet: The integrity, purity, and concentration of the isolated genomic DNA was determined by 2% agarose gel electrophoresis and a NanoDrop 2000 spectrophotometer (ThermoFisher). .. A double-digest was performed using 1 μg of DNA in a total volume of 30 μl with 5 units of Cvi AII at 25°C for 3 h and then with 5 units of Tse I at 65°C for 3 h in NEB CutSmart buffer (New England Biolabs).

    Article Title: microRNAs Establish Uniform Traits during the Architecture of Vertebrate Embryos
    Article Snippet: Mutagenesis was initially determined by restriction endonuclease assay using CviAII (NEB) for the miR-139 locus and T7 endonuclease I (NEB) for miR-24 and miR-223 genes. .. Briefly, 50 ng of genomic DNA isolated with the DNeasy Blood and Tissue kit (Qiagen) from a clutch of 15–20 injected 24 hpf embryos was used to amplify a 200–400 bp region spanning the intended mutation site.

    Labeling:

    Article Title: CTCF-mediated enhancer-promoter interaction is a critical regulator of cell-to-cell variation of gene expression
    Article Snippet: Chromatin was subsequently digested with 20 Units CviQ I (NEB), and 20 Units CviA II (NEB) at 25°C for 20 minutes, then with 20 Units Bfa I (NEB) at 37°C for 20 minutes. .. The DNA ends were blunted and labeled with biotin by Klenow enzyme in the presence of dCTP, dGTP, dTTP, biotin-14-dATP, followed by ligation using T4 DNA ligase.

    Purification:

    Article Title: Atypical Porcine Pestivirus: A Possible Cause of Congenital Tremor Type A-II in Newborn Piglets
    Article Snippet: .. Double-stranded DNA was purified by phenol/chloroform/isoamyl alcohol extraction and ethanol precipitation and digested with either MseI, Csp6I or CviAII restriction enzyme (New England Biolabs). .. Adaptors with different multiplex identifier sequences (MIDs) were ligated to the digested fragments of the different samples.

    Article Title: Genetic Diversity and Population Structure of the USDA Sweetpotato (Ipomoea batatas) Germplasm Collections Using GBSpoly
    Article Snippet: A double-digest was performed using 1 μg of DNA in a total volume of 30 μl with 5 units of Cvi AII at 25°C for 3 h and then with 5 units of Tse I at 65°C for 3 h in NEB CutSmart buffer (New England Biolabs). .. The digested DNA samples were purified with AMPure XP magnetic beads (ThermoFisher), quantified using a picogreen assay and then diluted to a concentration of 10 ng/μl.

    Article Title: Genetic Diversity and Population Structure of the USDA Sweetpotato (Ipomoea batatas) Germplasm Collections Using GBSpoly
    Article Snippet: A double-digest was performed using 1 μg of DNA in a total volume of 30 μl with 5 units of Cvi AII at 25°C for 3 h and then with 5 units of Tse I at 65°C for 3 h in NEB CutSmart buffer (New England Biolabs). .. The digested DNA samples were purified with AMPure XP magnetic beads (ThermoFisher), quantified using a picogreen assay and then diluted to a concentration of 10 ng/μl.

    Polymerase Chain Reaction:

    Article Title: Atypical Porcine Pestivirus: A Possible Cause of Congenital Tremor Type A-II in Newborn Piglets
    Article Snippet: Double-stranded DNA was purified by phenol/chloroform/isoamyl alcohol extraction and ethanol precipitation and digested with either MseI, Csp6I or CviAII restriction enzyme (New England Biolabs). .. Before PCR amplification, the fragments were purified with AMPure XP beads (Agencourt AMPure XP PCR, Beckman Coulter, Brea, CA, USA).

    Article Title: The RNA interactome of human telomerase RNA reveals a coding-independent role for a histone mRNA in telomere homeostasis
    Article Snippet: Briefly, 50 ng of genomic DNA was ligated with TeSLA-T oligonucleotides and then digested with CviAII, BfaI, NdeI, and MseI restriction enzymes (New England Biolabs), followed by shrimp alkaline phosphatase (New England Biolabs) treatment. .. The digested DNA was ligated with double-stranded TeSLA adapters, and 30 pg of the ligated DNA was subsequently used for long-range PCR amplifications.

    Article Title: The Maintenance of Telomere Length in CD28+ T Cells During T Lymphocyte Stimulation
    Article Snippet: After ligation, the genomic DNA is digested by a series of restriction enzymes including CviAII, BfaI, NdeI, and MseI (New England Biolabs) to digest all DNA but not telomeres which do not have restriction site for these enzymes. .. After adapter ligation, multiple PCR reactions are performed (initial melt at 94 °C for 2 minutes followed by 26 cycles of 94 °C for 15 seconds, 60 °C for 30 seconds, and 72 °C for 15 minutes) using 2.5 units of FailSafe enzyme mix (Epicenter) with 1× FailSafe buffer H in 25 μl reaction containing 0.25 μM primers and 20–40 pg of ligated DNA.

    Article Title: microRNAs Establish Uniform Traits during the Architecture of Vertebrate Embryos
    Article Snippet: Mutagenesis was initially determined by restriction endonuclease assay using CviAII (NEB) for the miR-139 locus and T7 endonuclease I (NEB) for miR-24 and miR-223 genes. .. Differential restriction endonuclease patterns of PCR amplicons indicated successful genome editing.

    Article Title: Multiplex amplification enabled by selective circularization of large sets of genomic DNA fragments
    Article Snippet: Two combinations of restriction enzymes, FH : 10 U of Fsp I (Fermentas) and 10 U of HpyCH4 V (New England Biolabs); AC : 10 U of Acu I (New England Biolabs) and 10 U of CviA II (New England Biolabs), were added to two different aliquots of 10 μg genomic DNA and 0.5 μg BSA, in a total volume of 50 μl NEBuffer 4. .. The circularization reactions were performed in PCR buffer (Invitrogen) supplemented with 10 mM MgCl2 , 1 mM NAD and 3.2 nM of vector oligonucleotide, using 2.5 U Platinum Taq DNA polymerase (Invitrogen) and 5 U Ampligase (Epicentre) in a volume of 25 μl.

    Article Title: Genetic association study from North India to analyze association of CYP19A1 and CYP17A1 with polycystic ovary syndrome
    Article Snippet: .. PCR amplification for rs2414096 (G > A) was done, using primers as given by Jin et al. [ ] CViAII (New England Biolabs) restriction enzyme digested PCR product when treated overnight at 37 °C. .. After digestion, a band of 189 bp for GG; 189-, 161-, and 28-bp band for GA; and 161- and 28-bp band for AA genotype were seen on 2.5% agarose gel.

    CRISPR:

    Article Title: microRNAs Establish Uniform Traits during the Architecture of Vertebrate Embryos
    Article Snippet: As previously described, TALENs were used to create miR-139 and miR-223 mutants and a multiplexed pool of CRISPR guide RNA pairs and Cas9 mRNA was used to simultaneously mutagenize all four miR-24 genes ( ; ). .. Mutagenesis was initially determined by restriction endonuclease assay using CviAII (NEB) for the miR-139 locus and T7 endonuclease I (NEB) for miR-24 and miR-223 genes.

    Plasmid Preparation:

    Article Title: Multiplex amplification enabled by selective circularization of large sets of genomic DNA fragments
    Article Snippet: Two combinations of restriction enzymes, FH : 10 U of Fsp I (Fermentas) and 10 U of HpyCH4 V (New England Biolabs); AC : 10 U of Acu I (New England Biolabs) and 10 U of CviA II (New England Biolabs), were added to two different aliquots of 10 μg genomic DNA and 0.5 μg BSA, in a total volume of 50 μl NEBuffer 4. .. The circularization reactions were performed in PCR buffer (Invitrogen) supplemented with 10 mM MgCl2 , 1 mM NAD and 3.2 nM of vector oligonucleotide, using 2.5 U Platinum Taq DNA polymerase (Invitrogen) and 5 U Ampligase (Epicentre) in a volume of 25 μl.

    In Vitro:

    Article Title: CTCF-mediated enhancer-promoter interaction is a critical regulator of cell-to-cell variation of gene expression
    Article Snippet: In vitro differentiated murine Th2 cells were cross-linked with 1% formaldehyde for 10 minutes at 25°C. .. Chromatin was subsequently digested with 20 Units CviQ I (NEB), and 20 Units CviA II (NEB) at 25°C for 20 minutes, then with 20 Units Bfa I (NEB) at 37°C for 20 minutes.

    Multiplex Assay:

    Article Title: Atypical Porcine Pestivirus: A Possible Cause of Congenital Tremor Type A-II in Newborn Piglets
    Article Snippet: Double-stranded DNA was purified by phenol/chloroform/isoamyl alcohol extraction and ethanol precipitation and digested with either MseI, Csp6I or CviAII restriction enzyme (New England Biolabs). .. Adaptors with different multiplex identifier sequences (MIDs) were ligated to the digested fragments of the different samples.

    Agarose Gel Electrophoresis:

    Article Title: Genetic Diversity and Population Structure of the USDA Sweetpotato (Ipomoea batatas) Germplasm Collections Using GBSpoly
    Article Snippet: A double-digest was performed using 1 μg of DNA in a total volume of 30 μl with 5 units of Cvi AII at 25°C for 3 h and then with 5 units of Tse I at 65°C for 3 h in NEB CutSmart buffer (New England Biolabs). .. A double-digest was performed using 1 μg of DNA in a total volume of 30 μl with 5 units of Cvi AII at 25°C for 3 h and then with 5 units of Tse I at 65°C for 3 h in NEB CutSmart buffer (New England Biolabs).

    Article Title: Genetic Diversity and Population Structure of the USDA Sweetpotato (Ipomoea batatas) Germplasm Collections Using GBSpoly
    Article Snippet: The integrity, purity, and concentration of the isolated genomic DNA was determined by 2% agarose gel electrophoresis and a NanoDrop 2000 spectrophotometer (ThermoFisher). .. A double-digest was performed using 1 μg of DNA in a total volume of 30 μl with 5 units of Cvi AII at 25°C for 3 h and then with 5 units of Tse I at 65°C for 3 h in NEB CutSmart buffer (New England Biolabs).

    Article Title: Genetic association study from North India to analyze association of CYP19A1 and CYP17A1 with polycystic ovary syndrome
    Article Snippet: CC genotype showed 173-bp band; CT showed 173-, 118-, and 55-bp band; and TT showed 118- and 55-bp band on agarose gel. .. PCR amplification for rs2414096 (G > A) was done, using primers as given by Jin et al. [ ] CViAII (New England Biolabs) restriction enzyme digested PCR product when treated overnight at 37 °C.

    In Situ:

    Article Title: CTCF-mediated enhancer-promoter interaction is a critical regulator of cell-to-cell variation of gene expression
    Article Snippet: The three enzyme Hi-C (3e Hi-C) was performed similar to in situ Hi-C ( ) with modifications as described below. .. Chromatin was subsequently digested with 20 Units CviQ I (NEB), and 20 Units CviA II (NEB) at 25°C for 20 minutes, then with 20 Units Bfa I (NEB) at 37°C for 20 minutes.

    Ethanol Precipitation:

    Article Title: Atypical Porcine Pestivirus: A Possible Cause of Congenital Tremor Type A-II in Newborn Piglets
    Article Snippet: .. Double-stranded DNA was purified by phenol/chloroform/isoamyl alcohol extraction and ethanol precipitation and digested with either MseI, Csp6I or CviAII restriction enzyme (New England Biolabs). .. Adaptors with different multiplex identifier sequences (MIDs) were ligated to the digested fragments of the different samples.

    Spectrophotometry:

    Article Title: Genetic Diversity and Population Structure of the USDA Sweetpotato (Ipomoea batatas) Germplasm Collections Using GBSpoly
    Article Snippet: A double-digest was performed using 1 μg of DNA in a total volume of 30 μl with 5 units of Cvi AII at 25°C for 3 h and then with 5 units of Tse I at 65°C for 3 h in NEB CutSmart buffer (New England Biolabs). .. A double-digest was performed using 1 μg of DNA in a total volume of 30 μl with 5 units of Cvi AII at 25°C for 3 h and then with 5 units of Tse I at 65°C for 3 h in NEB CutSmart buffer (New England Biolabs).

    Article Title: Genetic Diversity and Population Structure of the USDA Sweetpotato (Ipomoea batatas) Germplasm Collections Using GBSpoly
    Article Snippet: The integrity, purity, and concentration of the isolated genomic DNA was determined by 2% agarose gel electrophoresis and a NanoDrop 2000 spectrophotometer (ThermoFisher). .. A double-digest was performed using 1 μg of DNA in a total volume of 30 μl with 5 units of Cvi AII at 25°C for 3 h and then with 5 units of Tse I at 65°C for 3 h in NEB CutSmart buffer (New England Biolabs).

    Concentration Assay:

    Article Title: Genetic Diversity and Population Structure of the USDA Sweetpotato (Ipomoea batatas) Germplasm Collections Using GBSpoly
    Article Snippet: A double-digest was performed using 1 μg of DNA in a total volume of 30 μl with 5 units of Cvi AII at 25°C for 3 h and then with 5 units of Tse I at 65°C for 3 h in NEB CutSmart buffer (New England Biolabs). .. A double-digest was performed using 1 μg of DNA in a total volume of 30 μl with 5 units of Cvi AII at 25°C for 3 h and then with 5 units of Tse I at 65°C for 3 h in NEB CutSmart buffer (New England Biolabs).

    Article Title: Genetic Diversity and Population Structure of the USDA Sweetpotato (Ipomoea batatas) Germplasm Collections Using GBSpoly
    Article Snippet: The integrity, purity, and concentration of the isolated genomic DNA was determined by 2% agarose gel electrophoresis and a NanoDrop 2000 spectrophotometer (ThermoFisher). .. A double-digest was performed using 1 μg of DNA in a total volume of 30 μl with 5 units of Cvi AII at 25°C for 3 h and then with 5 units of Tse I at 65°C for 3 h in NEB CutSmart buffer (New England Biolabs).

    Lysis:

    Article Title: CTCF-mediated enhancer-promoter interaction is a critical regulator of cell-to-cell variation of gene expression
    Article Snippet: 10 × 106 cells were lysed in 10 ml lysis buffer (10 mM Tris-HCl pH8.0, 10 mM NaCl, 0.2% NP40; 10 μl protease inhibitors (Sigma)) with rotation at 4°C for 60 minutes. .. Chromatin was subsequently digested with 20 Units CviQ I (NEB), and 20 Units CviA II (NEB) at 25°C for 20 minutes, then with 20 Units Bfa I (NEB) at 37°C for 20 minutes.

    Fluorescence In Situ Hybridization:

    Article Title: microRNAs Establish Uniform Traits during the Architecture of Vertebrate Embryos
    Article Snippet: Mutagenesis was initially determined by restriction endonuclease assay using CviAII (NEB) for the miR-139 locus and T7 endonuclease I (NEB) for miR-24 and miR-223 genes. .. F0 founder fish were identified and outcrossed first with AB wild type and then with the Tg(kdrl:gfp)la116 strains to allow visualization of the developing vasculature.

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    New England Biolabs cviaii restriction enzyme
    Cviaii Restriction Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cviaii restriction enzyme/product/New England Biolabs
    Average 90 stars, based on 1 article reviews
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    cviaii restriction enzyme - by Bioz Stars, 2020-04
    90/100 stars
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