cviqi (New England Biolabs)

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Name:

CviQI

Description:

CviQI 10 000 units

Catalog Number:

r0639l

Price:

290

Category:

Restriction Enzymes

Size:

10 000 units

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New England Biolabs cviqi
CviQI

CviQI 10 000 units
https://www.bioz.com/result/cviqi/product/New England Biolabs
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99

cviqi - by Bioz Stars, 2021-03

93/100 stars

Images

1) Product Images from "Effect of valproic acid on mitochondrial epigenetics"

Article Title: Effect of valproic acid on mitochondrial epigenetics

Journal: European journal of pharmacology

doi: 10.1016/j.ejphar.2012.06.019

Representation of the selected mouse mitochondrial DNA regions analyzed with the beta-glu-5hmC-sensitive restriction enzymes CviAII and CviQI for 5hmC modifications. The presentation is based on the Mus musculus mitochondrion, complete genome 16299 residue.

Figure Legend Snippet: Representation of the selected mouse mitochondrial DNA regions analyzed with the beta-glu-5hmC-sensitive restriction enzymes CviAII and CviQI for 5hmC modifications. The presentation is based on the Mus musculus mitochondrion, complete genome 16299 residue.

Techniques Used:

Amplification:

Article Title: NBS1 rs2735383 polymorphism is associated with an increased risk of laryngeal carcinoma
Article Snippet: .. Meanwhile, the amplified fragment containing rs2735383 polymorphism was cut by Cvi QI (New England Biolabs, Beijing, China) at 25 °C for at least 3 h. The minor G allele produced a single 156-bp band, while the major C allele produced two separate bands of 57-bp and 99-bp. .. Total RNA from 32 laryngeal carcinoma tissues were extracted using TriPure Reagent (Roche Applied Science) and reverse transcribed to complementary DNA using cDNA synthesis kit ThermoScrept™ RT-PCR System (Invitrogen).

Produced:

Plasmid Preparation:

Article Title: Preparation of fluorescent in situ hybridisation probes without the need for optimisation of fragmentation
Article Snippet: .. Reagents Tryptone (ThermoFisher Scientific, Scoresby, VIC, AUS) Yeast Extract (ThermoFisher Scientific, Scoresby, VIC, AUS) Bacto Agar (ThermoFisher Scientific, Scoresby, VIC, AUS) Chloramphenicol (SIGMA, Castle Hill, NSW, AUS) TRIS (SIGMA, Castle Hill, NSW, AUS) EDTA (SIGMA, Castle Hill, NSW, AUS) RNAse (SIGMA, Castle Hill, NSW, AUS) SDS (SIGMA, Castle Hill, NSW, AUS) NaOH (SIGMA, Castle Hill, NSW, AUS) Guanidine Hydrochloride (GuHCl) (SIGMA, Castle Hill, NSW, AUS) Potassium Acetate (KAc) (SIGMA, Castle Hill, NSW, AUS) Potassium Chloride (SIGMA, Castle Hill, NSW, AUS) Sodium Chloride (SIGMA, Castle Hill, NSW, AUS) Trisodium citrate dihydrate (Na3 Citrate ⋅ 2H2 O) (SIGMA, Castle Hill, NSW, AUS) NP40 (SIGMA, Castle Hill, NSW, AUS) Acetic acid, glacial (Chem-supply, Gilman, SA, AUS) Isopropanol (Chem-supply, Gilman, SA, AUS) Ethanol (Chem-supply, Gilman, SA, AUS) QIAGEN™ plasmid mini kit (QIAGEN, Chadstone, VIC, AUS) CviQI (Genesearch, NEB, Arundel, QLD, AUS) AluI (Genesearch, NEB, Arundel, QLD, AUS) Phenol (SIGMA, Castle Hill, NSW, AUS) Chloroform (SIGMA, Castle Hill, NSW, AUS) Sodium Acetate trihydrate (NaAc) (SIGMA, Castle Hill, NSW, AUS) Glycogen (Ambion, ThermoFisher Scientific, Scoresby, VIC, AUS, cat#9510, 5 mg/ml) AMPure XP beads (Beckmann Coulter, Lane Cove, NSW, AUS) KREATECH ULS™ platinum bright labelling kit (Diagnostic Technology, Belrose, NSW, AUS) BIORAD P30 SSC spin columns (BIORAD, Gladesville, NSW) C0t1 DNA (species specific) (Life Technologies, ThermoFisher Scientific, Scoresby, VIC, AUS, 500 μg @ 1 mg/ml Cat# 15279-011 – for human) Hybridisation buffer Vysis LSI/WCP hybridisation buffer (Abbott, North Ryde, NSW, AUS, Cat# 06J67-001) DAPI II counterstain (antifade/mount) (Abbott, North Ryde, NSW, AUS, Cat# 06J50-001) .. BAC preparation 1 Streak BAC stock from glycerol or stab onto LB agar with appropriate selection antibiotic (for BACPAC CHORI RP11 clones use 10 μg/ml choloramphenicol) and grow overnight at 37 °C.

Article Title: Preparation of fluorescent in situ hybridisation probes without the need for optimisation of fragmentation
Article Snippet: .. Tryptone (ThermoFisher Scientific, Scoresby, VIC, AUS) Yeast Extract (ThermoFisher Scientific, Scoresby, VIC, AUS) Bacto Agar (ThermoFisher Scientific, Scoresby, VIC, AUS) Chloramphenicol (SIGMA, Castle Hill, NSW, AUS) TRIS (SIGMA, Castle Hill, NSW, AUS) EDTA (SIGMA, Castle Hill, NSW, AUS) RNAse (SIGMA, Castle Hill, NSW, AUS) SDS (SIGMA, Castle Hill, NSW, AUS) NaOH (SIGMA, Castle Hill, NSW, AUS) Guanidine Hydrochloride (GuHCl) (SIGMA, Castle Hill, NSW, AUS) Potassium Acetate (KAc) (SIGMA, Castle Hill, NSW, AUS) Potassium Chloride (SIGMA, Castle Hill, NSW, AUS) Sodium Chloride (SIGMA, Castle Hill, NSW, AUS) Trisodium citrate dihydrate (Na3 Citrate ⋅ 2H2 O) (SIGMA, Castle Hill, NSW, AUS) NP40 (SIGMA, Castle Hill, NSW, AUS) Acetic acid, glacial (Chem-supply, Gilman, SA, AUS) Isopropanol (Chem-supply, Gilman, SA, AUS) Ethanol (Chem-supply, Gilman, SA, AUS) QIAGEN™ plasmid mini kit (QIAGEN, Chadstone, VIC, AUS) CviQI (Genesearch, NEB, Arundel, QLD, AUS) AluI (Genesearch, NEB, Arundel, QLD, AUS) Phenol (SIGMA, Castle Hill, NSW, AUS) Chloroform (SIGMA, Castle Hill, NSW, AUS) Sodium Acetate trihydrate (NaAc) (SIGMA, Castle Hill, NSW, AUS) Glycogen (Ambion, ThermoFisher Scientific, Scoresby, VIC, AUS, cat#9510, 5 mg/ml) AMPure XP beads (Beckmann Coulter, Lane Cove, NSW, AUS) KREATECH ULS™ platinum bright labelling kit (Diagnostic Technology, Belrose, NSW, AUS) BIORAD P30 SSC spin columns (BIORAD, Gladesville, NSW) C0t1 DNA (species specific) (Life Technologies, ThermoFisher Scientific, Scoresby, VIC, AUS, 500 μg @ 1 mg/ml Cat# 15279-011 – for human) Hybridisation buffer Vysis LSI/WCP hybridisation buffer (Abbott, North Ryde, NSW, AUS, Cat# 06J67-001) DAPI II counterstain (antifade/mount) (Abbott, North Ryde, NSW, AUS, Cat# 06J50-001) .. 1 Streak BAC stock from glycerol or stab onto LB agar with appropriate selection antibiotic (for BACPAC CHORI RP11 clones use 10 μg/ml choloramphenicol) and grow overnight at 37 °C.

Chloramphenicol Acetyltransferase Assay:

Diagnostic Assay:

Hybridization:

Polymerase Chain Reaction:

Article Title: LINE-1-Derived Poly(A) Microsatellites Undergo Rapid Shortening and Create Somatic and Germline Mosaicism in Mice
Article Snippet: Both inverse PCR (iPCR) ( ) and splinkerette PCR ( ) were used for initial mapping of the genomic location of each L1 insertion. .. For splinkerette PCR, DNA from intronless animals was divided into 2 µg aliquots and digested overnight by five different enzymes: MspI, MseI, EcoRI, CviQI, and Sau3AI (New England Biolabs). .. After digestion, the DNA was ligated to synthetic adapters with compatible ends using T4 DNA ligase (New England Biolabs).

Ligation:

Article Title: Lineage-specific epigenomic and genomic activation of oncogene HNF4A promotes gastrointestinal adenocarcinomas.
Article Snippet: Digested DNA was next ligated using T4 DNA ligase (EL0013, Thermo 22 Scientific), followed by removal of cross-link using Proteinase K (19133, Qiagen), yielding 3C 23 libraries. .. These 3C libraries were subjected to a second enzyme digestion by Csp6i (R0639L, 24 NEB), followed by another ligation using T4 DNA ligase. .. A total of 3.2 µg of the resulting 4C 25 templates was used for a scale-up inverse nested PCR for 29 cycles.

Article Title: A combination strategy targeting enhancer plasticity exerts synergistic lethality against BETi-resistant leukemia cells
Article Snippet: DNA was purified by the traditional phenol-chloroform extraction method followed by ethanol precipitation. .. Purified DNA was then digested by BfaI (NEB R0639L, 50 units) overnight followed by a second ligation using T4 DNA ligase (NEB 0202 M, 6000 units) at 16 °C overnight. .. DNA products were ethanol precipitated and purified by the QIAquick PCR Purification Kit (Qiagen, 28104).

Sequencing:

Article Title: Effect of valproic acid on mitochondrial epigenetics
Article Snippet: For measurement of 5mC content, we used the corresponding Methylated DNA Quantification Kit from Epigentek. .. The sequence-specific 5hmC content in selected mtDNA regions was measured by qPCR applied to samples after a glucosyltransferase step followed by an enzymatic restriction digest with two enzymes, CviAII (CATG recognition site) and CviQI (GTAC recognition site) (New England Biolabs; Ipswich, MA) ( ). .. First, DNA samples are glucosylated with phage β-glucosyltransferase (T4-BGT; New England Biolabs, Ipswich, MA), which specifically transfers the glucose moiety of uridine diphosphoglucose (UDP-Glc) to the 5hmC residues but not to the 5mC residues.