cviqi (New England Biolabs)


Name:
CviQI
Description:
CviQI 10 000 units
Catalog Number:
r0639l
Price:
290
Category:
Restriction Enzymes
Size:
10 000 units
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Structured Review

CviQI 10 000 units
https://www.bioz.com/result/cviqi/product/New England Biolabs
Average 93 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Effect of valproic acid on mitochondrial epigenetics"
Article Title: Effect of valproic acid on mitochondrial epigenetics
Journal: European journal of pharmacology
doi: 10.1016/j.ejphar.2012.06.019

Figure Legend Snippet: Representation of the selected mouse mitochondrial DNA regions analyzed with the beta-glu-5hmC-sensitive restriction enzymes CviAII and CviQI for 5hmC modifications. The presentation is based on the Mus musculus mitochondrion, complete genome 16299 residue.
Techniques Used:
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Reagents Tryptone (ThermoFisher Scientific, Scoresby, VIC, AUS) Yeast Extract (ThermoFisher Scientific, Scoresby, VIC, AUS) Bacto Agar (ThermoFisher Scientific, Scoresby, VIC, AUS) Chloramphenicol (SIGMA, Castle Hill, NSW, AUS) TRIS (SIGMA, Castle Hill, NSW, AUS) EDTA (SIGMA, Castle Hill, NSW, AUS) RNAse (SIGMA, Castle Hill, NSW, AUS) SDS (SIGMA, Castle Hill, NSW, AUS) NaOH (SIGMA, Castle Hill, NSW, AUS) Guanidine Hydrochloride (GuHCl) (SIGMA, Castle Hill, NSW, AUS) Potassium Acetate (KAc) (SIGMA, Castle Hill, NSW, AUS) Potassium Chloride (SIGMA, Castle Hill, NSW, AUS) Sodium Chloride (SIGMA, Castle Hill, NSW, AUS) Trisodium citrate dihydrate (Na3 Citrate ⋅ 2H2 O) (SIGMA, Castle Hill, NSW, AUS) NP40 (SIGMA, Castle Hill, NSW, AUS) Acetic acid, glacial (Chem-supply, Gilman, SA, AUS) Isopropanol (Chem-supply, Gilman, SA, AUS) Ethanol (Chem-supply, Gilman, SA, AUS) QIAGEN™ plasmid mini kit (QIAGEN, Chadstone, VIC, AUS) Article Title: Preparation of fluorescent in situ hybridisation probes without the need for optimisation of fragmentation Article Snippet: .. Tryptone (ThermoFisher Scientific, Scoresby, VIC, AUS) Yeast Extract (ThermoFisher Scientific, Scoresby, VIC, AUS) Bacto Agar (ThermoFisher Scientific, Scoresby, VIC, AUS) Chloramphenicol (SIGMA, Castle Hill, NSW, AUS) TRIS (SIGMA, Castle Hill, NSW, AUS) EDTA (SIGMA, Castle Hill, NSW, AUS) RNAse (SIGMA, Castle Hill, NSW, AUS) SDS (SIGMA, Castle Hill, NSW, AUS) NaOH (SIGMA, Castle Hill, NSW, AUS) Guanidine Hydrochloride (GuHCl) (SIGMA, Castle Hill, NSW, AUS) Potassium Acetate (KAc) (SIGMA, Castle Hill, NSW, AUS) Potassium Chloride (SIGMA, Castle Hill, NSW, AUS) Sodium Chloride (SIGMA, Castle Hill, NSW, AUS) Trisodium citrate dihydrate (Na3 Citrate ⋅ 2H2 O) (SIGMA, Castle Hill, NSW, AUS) NP40 (SIGMA, Castle Hill, NSW, AUS) Acetic acid, glacial (Chem-supply, Gilman, SA, AUS) Isopropanol (Chem-supply, Gilman, SA, AUS) Ethanol (Chem-supply, Gilman, SA, AUS) QIAGEN™ plasmid mini kit (QIAGEN, Chadstone, VIC, AUS) Chloramphenicol Acetyltransferase Assay:Article Title: Preparation of fluorescent in situ hybridisation probes without the need for optimisation of fragmentation Article Snippet: .. 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Tryptone (ThermoFisher Scientific, Scoresby, VIC, AUS) Yeast Extract (ThermoFisher Scientific, Scoresby, VIC, AUS) Bacto Agar (ThermoFisher Scientific, Scoresby, VIC, AUS) Chloramphenicol (SIGMA, Castle Hill, NSW, AUS) TRIS (SIGMA, Castle Hill, NSW, AUS) EDTA (SIGMA, Castle Hill, NSW, AUS) RNAse (SIGMA, Castle Hill, NSW, AUS) SDS (SIGMA, Castle Hill, NSW, AUS) NaOH (SIGMA, Castle Hill, NSW, AUS) Guanidine Hydrochloride (GuHCl) (SIGMA, Castle Hill, NSW, AUS) Potassium Acetate (KAc) (SIGMA, Castle Hill, NSW, AUS) Potassium Chloride (SIGMA, Castle Hill, NSW, AUS) Sodium Chloride (SIGMA, Castle Hill, NSW, AUS) Trisodium citrate dihydrate (Na3 Citrate ⋅ 2H2 O) (SIGMA, Castle Hill, NSW, AUS) NP40 (SIGMA, Castle Hill, NSW, AUS) Acetic acid, glacial (Chem-supply, Gilman, SA, AUS) Isopropanol (Chem-supply, Gilman, SA, AUS) Ethanol (Chem-supply, Gilman, SA, AUS) QIAGEN™ plasmid mini kit (QIAGEN, Chadstone, VIC, AUS) Diagnostic Assay:Article Title: Preparation of fluorescent in situ hybridisation probes without the need for optimisation of fragmentation Article Snippet: .. Reagents Tryptone (ThermoFisher Scientific, Scoresby, VIC, AUS) Yeast Extract (ThermoFisher Scientific, Scoresby, VIC, AUS) Bacto Agar (ThermoFisher Scientific, Scoresby, VIC, AUS) Chloramphenicol (SIGMA, Castle Hill, NSW, AUS) TRIS (SIGMA, Castle Hill, NSW, AUS) EDTA (SIGMA, Castle Hill, NSW, AUS) RNAse (SIGMA, Castle Hill, NSW, AUS) SDS (SIGMA, Castle Hill, NSW, AUS) NaOH (SIGMA, Castle Hill, NSW, AUS) Guanidine Hydrochloride (GuHCl) (SIGMA, Castle Hill, NSW, AUS) Potassium Acetate (KAc) (SIGMA, Castle Hill, NSW, AUS) Potassium Chloride (SIGMA, Castle Hill, NSW, AUS) Sodium Chloride (SIGMA, Castle Hill, NSW, AUS) Trisodium citrate dihydrate (Na3 Citrate ⋅ 2H2 O) (SIGMA, Castle Hill, NSW, AUS) NP40 (SIGMA, Castle Hill, NSW, AUS) Acetic acid, glacial (Chem-supply, Gilman, SA, AUS) Isopropanol (Chem-supply, Gilman, SA, AUS) Ethanol (Chem-supply, Gilman, SA, AUS) QIAGEN™ plasmid mini kit (QIAGEN, Chadstone, VIC, AUS) Article Title: Preparation of fluorescent in situ hybridisation probes without the need for optimisation of fragmentation Article Snippet: .. Tryptone (ThermoFisher Scientific, Scoresby, VIC, AUS) Yeast Extract (ThermoFisher Scientific, Scoresby, VIC, AUS) Bacto Agar (ThermoFisher Scientific, Scoresby, VIC, AUS) Chloramphenicol (SIGMA, Castle Hill, NSW, AUS) TRIS (SIGMA, Castle Hill, NSW, AUS) EDTA (SIGMA, Castle Hill, NSW, AUS) RNAse (SIGMA, Castle Hill, NSW, AUS) SDS (SIGMA, Castle Hill, NSW, AUS) NaOH (SIGMA, Castle Hill, NSW, AUS) Guanidine Hydrochloride (GuHCl) (SIGMA, Castle Hill, NSW, AUS) Potassium Acetate (KAc) (SIGMA, Castle Hill, NSW, AUS) Potassium Chloride (SIGMA, Castle Hill, NSW, AUS) Sodium Chloride (SIGMA, Castle Hill, NSW, AUS) Trisodium citrate dihydrate (Na3 Citrate ⋅ 2H2 O) (SIGMA, Castle Hill, NSW, AUS) NP40 (SIGMA, Castle Hill, NSW, AUS) Acetic acid, glacial (Chem-supply, Gilman, SA, AUS) Isopropanol (Chem-supply, Gilman, SA, AUS) Ethanol (Chem-supply, Gilman, SA, AUS) QIAGEN™ plasmid mini kit (QIAGEN, Chadstone, VIC, AUS) Hybridization:Article Title: Preparation of fluorescent in situ hybridisation probes without the need for optimisation of fragmentation Article Snippet: .. Reagents Tryptone (ThermoFisher Scientific, Scoresby, VIC, AUS) Yeast Extract (ThermoFisher Scientific, Scoresby, VIC, AUS) Bacto Agar (ThermoFisher Scientific, Scoresby, VIC, AUS) Chloramphenicol (SIGMA, Castle Hill, NSW, AUS) TRIS (SIGMA, Castle Hill, NSW, AUS) EDTA (SIGMA, Castle Hill, NSW, AUS) RNAse (SIGMA, Castle Hill, NSW, AUS) SDS (SIGMA, Castle Hill, NSW, AUS) NaOH (SIGMA, Castle Hill, NSW, AUS) Guanidine Hydrochloride (GuHCl) (SIGMA, Castle Hill, NSW, AUS) Potassium Acetate (KAc) (SIGMA, Castle Hill, NSW, AUS) Potassium Chloride (SIGMA, Castle Hill, NSW, AUS) Sodium Chloride (SIGMA, Castle Hill, NSW, AUS) Trisodium citrate dihydrate (Na3 Citrate ⋅ 2H2 O) (SIGMA, Castle Hill, NSW, AUS) NP40 (SIGMA, Castle Hill, NSW, AUS) Acetic acid, glacial (Chem-supply, Gilman, SA, AUS) Isopropanol (Chem-supply, Gilman, SA, AUS) Ethanol (Chem-supply, Gilman, SA, AUS) QIAGEN™ plasmid mini kit (QIAGEN, Chadstone, VIC, AUS) Article Title: Preparation of fluorescent in situ hybridisation probes without the need for optimisation of fragmentation Article Snippet: .. Tryptone (ThermoFisher Scientific, Scoresby, VIC, AUS) Yeast Extract (ThermoFisher Scientific, Scoresby, VIC, AUS) Bacto Agar (ThermoFisher Scientific, Scoresby, VIC, AUS) Chloramphenicol (SIGMA, Castle Hill, NSW, AUS) TRIS (SIGMA, Castle Hill, NSW, AUS) EDTA (SIGMA, Castle Hill, NSW, AUS) RNAse (SIGMA, Castle Hill, NSW, AUS) SDS (SIGMA, Castle Hill, NSW, AUS) NaOH (SIGMA, Castle Hill, NSW, AUS) Guanidine Hydrochloride (GuHCl) (SIGMA, Castle Hill, NSW, AUS) Potassium Acetate (KAc) (SIGMA, Castle Hill, NSW, AUS) Potassium Chloride (SIGMA, Castle Hill, NSW, AUS) Sodium Chloride (SIGMA, Castle Hill, NSW, AUS) Trisodium citrate dihydrate (Na3 Citrate ⋅ 2H2 O) (SIGMA, Castle Hill, NSW, AUS) NP40 (SIGMA, Castle Hill, NSW, AUS) Acetic acid, glacial (Chem-supply, Gilman, SA, AUS) Isopropanol (Chem-supply, Gilman, SA, AUS) Ethanol (Chem-supply, Gilman, SA, AUS) QIAGEN™ plasmid mini kit (QIAGEN, Chadstone, VIC, AUS) Polymerase Chain Reaction:Article Title: LINE-1-Derived Poly(A) Microsatellites Undergo Rapid Shortening and Create Somatic and Germline Mosaicism in Mice Article Snippet: Both inverse PCR (iPCR) ( ) and splinkerette PCR ( ) were used for initial mapping of the genomic location of each L1 insertion. .. For splinkerette PCR, DNA from intronless animals was divided into 2 µg aliquots and digested overnight by five different enzymes: MspI, MseI, EcoRI, Ligation:Article Title: Lineage-specific epigenomic and genomic activation of oncogene HNF4A promotes gastrointestinal adenocarcinomas. Article Snippet: Digested DNA was next ligated using T4 DNA ligase (EL0013, Thermo 22 Scientific), followed by removal of cross-link using Proteinase K (19133, Qiagen), yielding 3C 23 libraries. .. These 3C libraries were subjected to a Article Title: A combination strategy targeting enhancer plasticity exerts synergistic lethality against BETi-resistant leukemia cells Article Snippet: DNA was purified by the traditional phenol-chloroform extraction method followed by ethanol precipitation. .. Purified DNA was then digested by Sequencing:Article Title: Effect of valproic acid on mitochondrial epigenetics Article Snippet: For measurement of 5mC content, we used the corresponding Methylated DNA Quantification Kit from Epigentek. .. The sequence-specific 5hmC content in selected mtDNA regions was measured by qPCR applied to samples after a glucosyltransferase step followed by an enzymatic restriction digest with two enzymes, CviAII (CATG recognition site) and Real-time Polymerase Chain Reaction:Article Title: Effect of valproic acid on mitochondrial epigenetics Article Snippet: For measurement of 5mC content, we used the corresponding Methylated DNA Quantification Kit from Epigentek. .. The sequence-specific 5hmC content in selected mtDNA regions was measured by qPCR applied to samples after a glucosyltransferase step followed by an enzymatic restriction digest with two enzymes, CviAII (CATG recognition site) and Purification:Article Title: A combination strategy targeting enhancer plasticity exerts synergistic lethality against BETi-resistant leukemia cells Article Snippet: DNA was purified by the traditional phenol-chloroform extraction method followed by ethanol precipitation. .. Purified DNA was then digested by |