cviqi  (New England Biolabs)


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    Name:
    CviQI
    Description:
    CviQI 10 000 units
    Catalog Number:
    R0639L
    Price:
    290
    Category:
    Restriction Enzymes
    Size:
    10 000 units
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    Structured Review

    New England Biolabs cviqi
    CviQI
    CviQI 10 000 units
    https://www.bioz.com/result/cviqi/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cviqi - by Bioz Stars, 2021-05
    95/100 stars

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    1) Product Images from "Effect of valproic acid on mitochondrial epigenetics"

    Article Title: Effect of valproic acid on mitochondrial epigenetics

    Journal: European journal of pharmacology

    doi: 10.1016/j.ejphar.2012.06.019

    Representation of the selected mouse mitochondrial DNA regions analyzed with the beta-glu-5hmC-sensitive restriction enzymes CviAII and CviQI for 5hmC modifications. The presentation is based on the Mus musculus mitochondrion, complete genome 16299 residue.
    Figure Legend Snippet: Representation of the selected mouse mitochondrial DNA regions analyzed with the beta-glu-5hmC-sensitive restriction enzymes CviAII and CviQI for 5hmC modifications. The presentation is based on the Mus musculus mitochondrion, complete genome 16299 residue.

    Techniques Used:

    Related Articles

    Sequencing:

    Article Title: Effect of valproic acid on mitochondrial epigenetics
    Article Snippet: For measurement of 5mC content, we used the corresponding Methylated DNA Quantification Kit from Epigentek. .. The sequence-specific 5hmC content in selected mtDNA regions was measured by qPCR applied to samples after a glucosyltransferase step followed by an enzymatic restriction digest with two enzymes, CviAII (CATG recognition site) and CviQI (GTAC recognition site) (New England Biolabs; Ipswich, MA) ( ). .. First, DNA samples are glucosylated with phage β-glucosyltransferase (T4-BGT; New England Biolabs, Ipswich, MA), which specifically transfers the glucose moiety of uridine diphosphoglucose (UDP-Glc) to the 5hmC residues but not to the 5mC residues.

    Article Title: The evolutionary dynamics of genetic incompatibilities introduced by duplicated genes in Arabidopsis thaliana
    Article Snippet: RAD-seq sequencing libraries were prepared as described ( ) with modifications. .. Per genotype, 500 ng DNA were digested with 10 units of CviQI (NEB, cutting site G’TAC) at 25°C for 2 h. The number of expected cutting sites was estimated around 236,000 based on the Col-0 genome sequence. .. Cut DNA was purified using 96 DNA clean and concentrator kit (Zymo) a diluted in 25 µl EB.

    Real-time Polymerase Chain Reaction:

    Article Title: Effect of valproic acid on mitochondrial epigenetics
    Article Snippet: For measurement of 5mC content, we used the corresponding Methylated DNA Quantification Kit from Epigentek. .. The sequence-specific 5hmC content in selected mtDNA regions was measured by qPCR applied to samples after a glucosyltransferase step followed by an enzymatic restriction digest with two enzymes, CviAII (CATG recognition site) and CviQI (GTAC recognition site) (New England Biolabs; Ipswich, MA) ( ). .. First, DNA samples are glucosylated with phage β-glucosyltransferase (T4-BGT; New England Biolabs, Ipswich, MA), which specifically transfers the glucose moiety of uridine diphosphoglucose (UDP-Glc) to the 5hmC residues but not to the 5mC residues.

    Plasmid Preparation:

    Article Title: Preparation of fluorescent in situ hybridisation probes without the need for optimisation of fragmentation
    Article Snippet: .. Tryptone (ThermoFisher Scientific, Scoresby, VIC, AUS) Yeast Extract (ThermoFisher Scientific, Scoresby, VIC, AUS) Bacto Agar (ThermoFisher Scientific, Scoresby, VIC, AUS) Chloramphenicol (SIGMA, Castle Hill, NSW, AUS) TRIS (SIGMA, Castle Hill, NSW, AUS) EDTA (SIGMA, Castle Hill, NSW, AUS) RNAse (SIGMA, Castle Hill, NSW, AUS) SDS (SIGMA, Castle Hill, NSW, AUS) NaOH (SIGMA, Castle Hill, NSW, AUS) Guanidine Hydrochloride (GuHCl) (SIGMA, Castle Hill, NSW, AUS) Potassium Acetate (KAc) (SIGMA, Castle Hill, NSW, AUS) Potassium Chloride (SIGMA, Castle Hill, NSW, AUS) Sodium Chloride (SIGMA, Castle Hill, NSW, AUS) Trisodium citrate dihydrate (Na3 Citrate ⋅ 2H2 O) (SIGMA, Castle Hill, NSW, AUS) NP40 (SIGMA, Castle Hill, NSW, AUS) Acetic acid, glacial (Chem-supply, Gilman, SA, AUS) Isopropanol (Chem-supply, Gilman, SA, AUS) Ethanol (Chem-supply, Gilman, SA, AUS) QIAGEN™ plasmid mini kit (QIAGEN, Chadstone, VIC, AUS) CviQI (Genesearch, NEB, Arundel, QLD, AUS) AluI (Genesearch, NEB, Arundel, QLD, AUS) Phenol (SIGMA, Castle Hill, NSW, AUS) Chloroform (SIGMA, Castle Hill, NSW, AUS) Sodium Acetate trihydrate (NaAc) (SIGMA, Castle Hill, NSW, AUS) Glycogen (Ambion, ThermoFisher Scientific, Scoresby, VIC, AUS, cat#9510, 5 mg/ml) AMPure XP beads (Beckmann Coulter, Lane Cove, NSW, AUS) KREATECH ULS™ platinum bright labelling kit (Diagnostic Technology, Belrose, NSW, AUS) BIORAD P30 SSC spin columns (BIORAD, Gladesville, NSW) C0t1 DNA (species specific) (Life Technologies, ThermoFisher Scientific, Scoresby, VIC, AUS, 500 μg @ 1 mg/ml Cat# 15279-011 – for human) Hybridisation buffer Vysis LSI/WCP hybridisation buffer (Abbott, North Ryde, NSW, AUS, Cat# 06J67-001) DAPI II counterstain (antifade/mount) (Abbott, North Ryde, NSW, AUS, Cat# 06J50-001) .. 1 Streak BAC stock from glycerol or stab onto LB agar with appropriate selection antibiotic (for BACPAC CHORI RP11 clones use 10 μg/ml choloramphenicol) and grow overnight at 37 °C.

    Chloramphenicol Acetyltransferase Assay:

    Article Title: Preparation of fluorescent in situ hybridisation probes without the need for optimisation of fragmentation
    Article Snippet: .. Tryptone (ThermoFisher Scientific, Scoresby, VIC, AUS) Yeast Extract (ThermoFisher Scientific, Scoresby, VIC, AUS) Bacto Agar (ThermoFisher Scientific, Scoresby, VIC, AUS) Chloramphenicol (SIGMA, Castle Hill, NSW, AUS) TRIS (SIGMA, Castle Hill, NSW, AUS) EDTA (SIGMA, Castle Hill, NSW, AUS) RNAse (SIGMA, Castle Hill, NSW, AUS) SDS (SIGMA, Castle Hill, NSW, AUS) NaOH (SIGMA, Castle Hill, NSW, AUS) Guanidine Hydrochloride (GuHCl) (SIGMA, Castle Hill, NSW, AUS) Potassium Acetate (KAc) (SIGMA, Castle Hill, NSW, AUS) Potassium Chloride (SIGMA, Castle Hill, NSW, AUS) Sodium Chloride (SIGMA, Castle Hill, NSW, AUS) Trisodium citrate dihydrate (Na3 Citrate ⋅ 2H2 O) (SIGMA, Castle Hill, NSW, AUS) NP40 (SIGMA, Castle Hill, NSW, AUS) Acetic acid, glacial (Chem-supply, Gilman, SA, AUS) Isopropanol (Chem-supply, Gilman, SA, AUS) Ethanol (Chem-supply, Gilman, SA, AUS) QIAGEN™ plasmid mini kit (QIAGEN, Chadstone, VIC, AUS) CviQI (Genesearch, NEB, Arundel, QLD, AUS) AluI (Genesearch, NEB, Arundel, QLD, AUS) Phenol (SIGMA, Castle Hill, NSW, AUS) Chloroform (SIGMA, Castle Hill, NSW, AUS) Sodium Acetate trihydrate (NaAc) (SIGMA, Castle Hill, NSW, AUS) Glycogen (Ambion, ThermoFisher Scientific, Scoresby, VIC, AUS, cat#9510, 5 mg/ml) AMPure XP beads (Beckmann Coulter, Lane Cove, NSW, AUS) KREATECH ULS™ platinum bright labelling kit (Diagnostic Technology, Belrose, NSW, AUS) BIORAD P30 SSC spin columns (BIORAD, Gladesville, NSW) C0t1 DNA (species specific) (Life Technologies, ThermoFisher Scientific, Scoresby, VIC, AUS, 500 μg @ 1 mg/ml Cat# 15279-011 – for human) Hybridisation buffer Vysis LSI/WCP hybridisation buffer (Abbott, North Ryde, NSW, AUS, Cat# 06J67-001) DAPI II counterstain (antifade/mount) (Abbott, North Ryde, NSW, AUS, Cat# 06J50-001) .. 1 Streak BAC stock from glycerol or stab onto LB agar with appropriate selection antibiotic (for BACPAC CHORI RP11 clones use 10 μg/ml choloramphenicol) and grow overnight at 37 °C.

    Diagnostic Assay:

    Article Title: Preparation of fluorescent in situ hybridisation probes without the need for optimisation of fragmentation
    Article Snippet: .. Tryptone (ThermoFisher Scientific, Scoresby, VIC, AUS) Yeast Extract (ThermoFisher Scientific, Scoresby, VIC, AUS) Bacto Agar (ThermoFisher Scientific, Scoresby, VIC, AUS) Chloramphenicol (SIGMA, Castle Hill, NSW, AUS) TRIS (SIGMA, Castle Hill, NSW, AUS) EDTA (SIGMA, Castle Hill, NSW, AUS) RNAse (SIGMA, Castle Hill, NSW, AUS) SDS (SIGMA, Castle Hill, NSW, AUS) NaOH (SIGMA, Castle Hill, NSW, AUS) Guanidine Hydrochloride (GuHCl) (SIGMA, Castle Hill, NSW, AUS) Potassium Acetate (KAc) (SIGMA, Castle Hill, NSW, AUS) Potassium Chloride (SIGMA, Castle Hill, NSW, AUS) Sodium Chloride (SIGMA, Castle Hill, NSW, AUS) Trisodium citrate dihydrate (Na3 Citrate ⋅ 2H2 O) (SIGMA, Castle Hill, NSW, AUS) NP40 (SIGMA, Castle Hill, NSW, AUS) Acetic acid, glacial (Chem-supply, Gilman, SA, AUS) Isopropanol (Chem-supply, Gilman, SA, AUS) Ethanol (Chem-supply, Gilman, SA, AUS) QIAGEN™ plasmid mini kit (QIAGEN, Chadstone, VIC, AUS) CviQI (Genesearch, NEB, Arundel, QLD, AUS) AluI (Genesearch, NEB, Arundel, QLD, AUS) Phenol (SIGMA, Castle Hill, NSW, AUS) Chloroform (SIGMA, Castle Hill, NSW, AUS) Sodium Acetate trihydrate (NaAc) (SIGMA, Castle Hill, NSW, AUS) Glycogen (Ambion, ThermoFisher Scientific, Scoresby, VIC, AUS, cat#9510, 5 mg/ml) AMPure XP beads (Beckmann Coulter, Lane Cove, NSW, AUS) KREATECH ULS™ platinum bright labelling kit (Diagnostic Technology, Belrose, NSW, AUS) BIORAD P30 SSC spin columns (BIORAD, Gladesville, NSW) C0t1 DNA (species specific) (Life Technologies, ThermoFisher Scientific, Scoresby, VIC, AUS, 500 μg @ 1 mg/ml Cat# 15279-011 – for human) Hybridisation buffer Vysis LSI/WCP hybridisation buffer (Abbott, North Ryde, NSW, AUS, Cat# 06J67-001) DAPI II counterstain (antifade/mount) (Abbott, North Ryde, NSW, AUS, Cat# 06J50-001) .. 1 Streak BAC stock from glycerol or stab onto LB agar with appropriate selection antibiotic (for BACPAC CHORI RP11 clones use 10 μg/ml choloramphenicol) and grow overnight at 37 °C.

    Hybridization:

    Article Title: Preparation of fluorescent in situ hybridisation probes without the need for optimisation of fragmentation
    Article Snippet: .. Tryptone (ThermoFisher Scientific, Scoresby, VIC, AUS) Yeast Extract (ThermoFisher Scientific, Scoresby, VIC, AUS) Bacto Agar (ThermoFisher Scientific, Scoresby, VIC, AUS) Chloramphenicol (SIGMA, Castle Hill, NSW, AUS) TRIS (SIGMA, Castle Hill, NSW, AUS) EDTA (SIGMA, Castle Hill, NSW, AUS) RNAse (SIGMA, Castle Hill, NSW, AUS) SDS (SIGMA, Castle Hill, NSW, AUS) NaOH (SIGMA, Castle Hill, NSW, AUS) Guanidine Hydrochloride (GuHCl) (SIGMA, Castle Hill, NSW, AUS) Potassium Acetate (KAc) (SIGMA, Castle Hill, NSW, AUS) Potassium Chloride (SIGMA, Castle Hill, NSW, AUS) Sodium Chloride (SIGMA, Castle Hill, NSW, AUS) Trisodium citrate dihydrate (Na3 Citrate ⋅ 2H2 O) (SIGMA, Castle Hill, NSW, AUS) NP40 (SIGMA, Castle Hill, NSW, AUS) Acetic acid, glacial (Chem-supply, Gilman, SA, AUS) Isopropanol (Chem-supply, Gilman, SA, AUS) Ethanol (Chem-supply, Gilman, SA, AUS) QIAGEN™ plasmid mini kit (QIAGEN, Chadstone, VIC, AUS) CviQI (Genesearch, NEB, Arundel, QLD, AUS) AluI (Genesearch, NEB, Arundel, QLD, AUS) Phenol (SIGMA, Castle Hill, NSW, AUS) Chloroform (SIGMA, Castle Hill, NSW, AUS) Sodium Acetate trihydrate (NaAc) (SIGMA, Castle Hill, NSW, AUS) Glycogen (Ambion, ThermoFisher Scientific, Scoresby, VIC, AUS, cat#9510, 5 mg/ml) AMPure XP beads (Beckmann Coulter, Lane Cove, NSW, AUS) KREATECH ULS™ platinum bright labelling kit (Diagnostic Technology, Belrose, NSW, AUS) BIORAD P30 SSC spin columns (BIORAD, Gladesville, NSW) C0t1 DNA (species specific) (Life Technologies, ThermoFisher Scientific, Scoresby, VIC, AUS, 500 μg @ 1 mg/ml Cat# 15279-011 – for human) Hybridisation buffer Vysis LSI/WCP hybridisation buffer (Abbott, North Ryde, NSW, AUS, Cat# 06J67-001) DAPI II counterstain (antifade/mount) (Abbott, North Ryde, NSW, AUS, Cat# 06J50-001) .. 1 Streak BAC stock from glycerol or stab onto LB agar with appropriate selection antibiotic (for BACPAC CHORI RP11 clones use 10 μg/ml choloramphenicol) and grow overnight at 37 °C.

    Purification:

    Article Title: A combination strategy targeting enhancer plasticity exerts synergistic lethality against BETi-resistant leukemia cells
    Article Snippet: DNA was purified by the traditional phenol-chloroform extraction method followed by ethanol precipitation. .. Purified DNA was then digested by BfaI (NEB R0639L, 50 units) overnight followed by a second ligation using T4 DNA ligase (NEB 0202 M, 6000 units) at 16 °C overnight. .. DNA products were ethanol precipitated and purified by the QIAquick PCR Purification Kit (Qiagen, 28104).

    Ligation:

    Article Title: A combination strategy targeting enhancer plasticity exerts synergistic lethality against BETi-resistant leukemia cells
    Article Snippet: DNA was purified by the traditional phenol-chloroform extraction method followed by ethanol precipitation. .. Purified DNA was then digested by BfaI (NEB R0639L, 50 units) overnight followed by a second ligation using T4 DNA ligase (NEB 0202 M, 6000 units) at 16 °C overnight. .. DNA products were ethanol precipitated and purified by the QIAquick PCR Purification Kit (Qiagen, 28104).

    Modification:

    Article Title: Effect of aging on 5-hydroxymethylcytosine in brain mitochondria
    Article Snippet: The 5hmC modifications of mtDNA were quantified using a glucosyltransferase assay that involves an enzymatic restriction digest combined with qPCR; the principle of the method described and visualized in . .. We have modified this assay by selecting a different set of restriction enzymes, i.e., CviAII (CATG recognition site) and CviQI (GTAC recognition site) (New England Biolabs, Ipswich, MA, USA). .. Both enzymes are insensitive to cytosine methylation and hydroxymethylation (CviAII is only partially, about 25% sensitive to 5hmC).

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    • cviqi  (New England Biolabs)
    95
    New England Biolabs cviqi
    Representation of the selected mouse mitochondrial DNA regions analyzed with the beta-glu-5hmC-sensitive restriction enzymes <t>CviAII</t> and <t>CviQI</t> for 5hmC modifications. The presentation is based on the Mus musculus mitochondrion, complete genome 16299 residue.
    Cviqi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cviqi/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cviqi - by Bioz Stars, 2021-05
    95/100 stars
    		  Buy from Supplier

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    Representation of the selected mouse mitochondrial DNA regions analyzed with the beta-glu-5hmC-sensitive restriction enzymes CviAII and CviQI for 5hmC modifications. The presentation is based on the Mus musculus mitochondrion, complete genome 16299 residue.

    Journal: European journal of pharmacology

    Article Title: Effect of valproic acid on mitochondrial epigenetics

    doi: 10.1016/j.ejphar.2012.06.019

    Figure Lengend Snippet: Representation of the selected mouse mitochondrial DNA regions analyzed with the beta-glu-5hmC-sensitive restriction enzymes CviAII and CviQI for 5hmC modifications. The presentation is based on the Mus musculus mitochondrion, complete genome 16299 residue.

    Article Snippet: The sequence-specific 5hmC content in selected mtDNA regions was measured by qPCR applied to samples after a glucosyltransferase step followed by an enzymatic restriction digest with two enzymes, CviAII (CATG recognition site) and CviQI (GTAC recognition site) (New England Biolabs; Ipswich, MA) ( ).

    Techniques: