bseyi  (New England Biolabs)


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    Name:
    BseYI
    Description:
    BseYI 500 units
    Catalog Number:
    r0635l
    Price:
    282
    Category:
    Restriction Enzymes
    Size:
    500 units
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    Structured Review

    New England Biolabs bseyi
    BseYI
    BseYI 500 units
    https://www.bioz.com/result/bseyi/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bseyi - by Bioz Stars, 2021-03
    94/100 stars

    Images

    1) Product Images from "Altered 3D chromatin structure permits inversional recombination at the IgH locus"

    Article Title: Altered 3D chromatin structure permits inversional recombination at the IgH locus

    Journal: Science Advances

    doi: 10.1126/sciadv.aaz8850

    Inversional and deletional recombination of 3′-RSS of DQ52 and DSP2 on WT and IGCR1-mutated IgH alleles. Schematic representation of 3′-RSS of DQ52 ( A ) and DSP2 ( B ) rearrangements to V H 81X gene segment by inversion (black arrows) or to J H s gene segments by deletion (blue arrows), respectively (left). Products of each form of rearrangements are shown to the right. RAG2-deficient pro–B cell lines with WT or IGCR1-mutated IgH alleles [CBE −/− (1) and CBE −/− (2)] were infected with a Rag2-expressing lentivirus, followed by genomic DNA purification after 14 days of selection with puromycin. LAM-HTGTS experiments were carried out as previously described ( 33 , 39 ) with baits (red arrows) located 50- to 100-bp upstream of DQ52 (A) or DSP2 (B). Restriction enzyme Sacl-HF (R3156S, NEB) and BseYI (R0635S, NEB) were used to remove germline DNA with DQ52 and DSP2 as bait, respectively. Total reads were aligned to detect recombination by deletion to J H s and by inversion to V H 81X. The lower reads of 3′ DSP2 RSS utilization compared to DQ52 gene may be due to inefficient restriction of germline DSP2 fragments during library preparation. Average reads and percentages from two independent experiments are shown in red.
    Figure Legend Snippet: Inversional and deletional recombination of 3′-RSS of DQ52 and DSP2 on WT and IGCR1-mutated IgH alleles. Schematic representation of 3′-RSS of DQ52 ( A ) and DSP2 ( B ) rearrangements to V H 81X gene segment by inversion (black arrows) or to J H s gene segments by deletion (blue arrows), respectively (left). Products of each form of rearrangements are shown to the right. RAG2-deficient pro–B cell lines with WT or IGCR1-mutated IgH alleles [CBE −/− (1) and CBE −/− (2)] were infected with a Rag2-expressing lentivirus, followed by genomic DNA purification after 14 days of selection with puromycin. LAM-HTGTS experiments were carried out as previously described ( 33 , 39 ) with baits (red arrows) located 50- to 100-bp upstream of DQ52 (A) or DSP2 (B). Restriction enzyme Sacl-HF (R3156S, NEB) and BseYI (R0635S, NEB) were used to remove germline DNA with DQ52 and DSP2 as bait, respectively. Total reads were aligned to detect recombination by deletion to J H s and by inversion to V H 81X. The lower reads of 3′ DSP2 RSS utilization compared to DQ52 gene may be due to inefficient restriction of germline DSP2 fragments during library preparation. Average reads and percentages from two independent experiments are shown in red.

    Techniques Used: Infection, Expressing, DNA Purification, Selection, Laser Capture Microdissection

    2) Product Images from "A tale of two haplotype groups: Evaluating the New World Junonia ring species hypothesis using the distribution of divergent COI haplotypes"

    Article Title: A tale of two haplotype groups: Evaluating the New World Junonia ring species hypothesis using the distribution of divergent COI haplotypes

    Journal: Systematic entomology

    doi: 10.1111/syen.12120

    Map of PCR primer binding sites and restriction enzyme cut sizes for the barcode fragment of COI. BamHI restriction sites are only found in haplotype group A alleles while AflIII and BseYI restriction sites are only found in haplotype group B alleles.
    Figure Legend Snippet: Map of PCR primer binding sites and restriction enzyme cut sizes for the barcode fragment of COI. BamHI restriction sites are only found in haplotype group A alleles while AflIII and BseYI restriction sites are only found in haplotype group B alleles.

    Techniques Used: Polymerase Chain Reaction, Binding Assay

    Related Articles

    Agarose Gel Electrophoresis:

    Article Title: MATN3 Gene Polymorphism Is Associated with Osteoarthritis in Chinese Han Population: A Community-Based Case-Control Study
    Article Snippet: .. 10 μ L of 501-bp product were then digested with 10 units of BSEYI restriction enzyme (NEB-R0635S) at 37°C for 10 h. Digestion products were visualized on a 3% agarose gel containing ethidium bromide. ..

    Article Title: Clinical significance of Matrilin-3 gene polymorphism in Egyptian patients with primary knee osteoarthritis
    Article Snippet: .. In total, 10 μ L of 501-bp product were digested with 10 units of BSEYI restriction enzyme (NEB-R0635S, Lot:0181412 USA) at 37 ° C for 10 h. Digestion products were visualized on a 2.5% agarose gel containing 2% ethidium bromide. ..

    Polymerase Chain Reaction:

    Article Title: A study of the relationships between KLF2 polymorphisms and body weight control in a French population
    Article Snippet: The PCR reaction was performed using 1 mM MgCl2 at an annealing temperature of 60°C. .. The PCR product (348 bp) was digested with 1 unit of BseY I (New England Biolabs, Hertfordshire, UK). ..

    Article Title: Variants in Circadian Rhythm Gene Cry1 Interacts with Healthy Dietary Pattern for Serum Leptin Levels: a Cross-sectional Study
    Article Snippet: The PCR proccess was conducted in 5 steps of 1) 4 minutes initial denaturation (94°C), 2) 30 seconds denaturation (94°C), 3) 30 seconds annealing (58°C), 4) 30 seconds extension (72°C), and 5) 5 minutes final extension (72°C); denaturation, annealing, and extension were done for 35 cycles and the rest of the steps were done once. .. PCR products were digested using BseYI (cataloge number: R0635S; New England Biolabs, Essex, MA, USA) which yielded 2 cuts and 3 fragments of 108, 50, and 226 base pairs (bps); and one cut and 2 fragments of 156 and 226 bps in the presence of G and C alleles, respectively. ..

    Article Title: Mutation I810N in the \u03b13 isoform of Na+,K+-ATPase causes impairments in the sodium pump and hyperexcitability in the CNS
    Article Snippet: BAC DNA was injected into the pronucleus of (C57BL/6 × SJL) F2 embryos, which were then transferred into the oviducts of 0.5 day pseudopregnant CD-1 females (Charles River Laboratories). .. From among the 154 pups born, 6 BAC transgenic pups were identified by PCR amplification across the SP6 and T7 vector-insert boundaries and by the presence of a Bse YI (New England BioLabs) restriction site (ss60981659; 5′-C*CCAGC-3′). .. Germline transmission and good breeding performance were established in 1 line.

    Article Title: A tale of two haplotype groups: Evaluating the New World Junonia ring species hypothesis using the distribution of divergent COI haplotypes
    Article Snippet: PCR reactions were prepared using reaction volumes of 25 µl and Qiagen TopTaq Mastermix for these and all other PCR re-amplifications described below. .. PCR products of the correct size were evaluated by a triple digest using BseYI, AflIII, and BamHI restriction endonucleases (New England Biolabs, Ipswich, MA, USA) ( ). .. A 10 µL aliquot of the PCR product was incubated with 10 µL of the triple digest cocktail (2 µL of NEB Buffer3, 2 µL of BSA (10×, 1 mg/mL), 4 µL of ddH2 O, 0.5 µL of BseYI, 0.5 µL of AflIII and 1 µL of BamHI) for 1 hour in a 37°C water bath.

    BAC Assay:

    Article Title: Mutation I810N in the \u03b13 isoform of Na+,K+-ATPase causes impairments in the sodium pump and hyperexcitability in the CNS
    Article Snippet: BAC DNA was injected into the pronucleus of (C57BL/6 × SJL) F2 embryos, which were then transferred into the oviducts of 0.5 day pseudopregnant CD-1 females (Charles River Laboratories). .. From among the 154 pups born, 6 BAC transgenic pups were identified by PCR amplification across the SP6 and T7 vector-insert boundaries and by the presence of a Bse YI (New England BioLabs) restriction site (ss60981659; 5′-C*CCAGC-3′). .. Germline transmission and good breeding performance were established in 1 line.

    Transgenic Assay:

    Article Title: Mutation I810N in the \u03b13 isoform of Na+,K+-ATPase causes impairments in the sodium pump and hyperexcitability in the CNS
    Article Snippet: BAC DNA was injected into the pronucleus of (C57BL/6 × SJL) F2 embryos, which were then transferred into the oviducts of 0.5 day pseudopregnant CD-1 females (Charles River Laboratories). .. From among the 154 pups born, 6 BAC transgenic pups were identified by PCR amplification across the SP6 and T7 vector-insert boundaries and by the presence of a Bse YI (New England BioLabs) restriction site (ss60981659; 5′-C*CCAGC-3′). .. Germline transmission and good breeding performance were established in 1 line.

    Amplification:

    Article Title: Mutation I810N in the \u03b13 isoform of Na+,K+-ATPase causes impairments in the sodium pump and hyperexcitability in the CNS
    Article Snippet: BAC DNA was injected into the pronucleus of (C57BL/6 × SJL) F2 embryos, which were then transferred into the oviducts of 0.5 day pseudopregnant CD-1 females (Charles River Laboratories). .. From among the 154 pups born, 6 BAC transgenic pups were identified by PCR amplification across the SP6 and T7 vector-insert boundaries and by the presence of a Bse YI (New England BioLabs) restriction site (ss60981659; 5′-C*CCAGC-3′). .. Germline transmission and good breeding performance were established in 1 line.

    Plasmid Preparation:

    Article Title: Mutation I810N in the \u03b13 isoform of Na+,K+-ATPase causes impairments in the sodium pump and hyperexcitability in the CNS
    Article Snippet: BAC DNA was injected into the pronucleus of (C57BL/6 × SJL) F2 embryos, which were then transferred into the oviducts of 0.5 day pseudopregnant CD-1 females (Charles River Laboratories). .. From among the 154 pups born, 6 BAC transgenic pups were identified by PCR amplification across the SP6 and T7 vector-insert boundaries and by the presence of a Bse YI (New England BioLabs) restriction site (ss60981659; 5′-C*CCAGC-3′). .. Germline transmission and good breeding performance were established in 1 line.

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    New England Biolabs bseyi
    Inversional and deletional recombination of 3′-RSS of DQ52 and DSP2 on WT and IGCR1-mutated IgH alleles. Schematic representation of 3′-RSS of DQ52 ( A ) and DSP2 ( B ) rearrangements to V H 81X gene segment by inversion (black arrows) or to J H s gene segments by deletion (blue arrows), respectively (left). Products of each form of rearrangements are shown to the right. RAG2-deficient pro–B cell lines with WT or IGCR1-mutated IgH alleles [CBE −/− (1) and CBE −/− (2)] were infected with a Rag2-expressing lentivirus, followed by genomic DNA purification after 14 days of selection with puromycin. LAM-HTGTS experiments were carried out as previously described ( 33 , 39 ) with baits (red arrows) located 50- to 100-bp upstream of DQ52 (A) or DSP2 (B). Restriction enzyme Sacl-HF (R3156S, NEB) and <t>BseYI</t> (R0635S, NEB) were used to remove <t>germline</t> DNA with DQ52 and DSP2 as bait, respectively. Total reads were aligned to detect recombination by deletion to J H s and by inversion to V H 81X. The lower reads of 3′ DSP2 RSS utilization compared to DQ52 gene may be due to inefficient restriction of germline DSP2 fragments during library preparation. Average reads and percentages from two independent experiments are shown in red.
    Bseyi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bseyi/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bseyi - by Bioz Stars, 2021-03
    94/100 stars
      Buy from Supplier

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    Inversional and deletional recombination of 3′-RSS of DQ52 and DSP2 on WT and IGCR1-mutated IgH alleles. Schematic representation of 3′-RSS of DQ52 ( A ) and DSP2 ( B ) rearrangements to V H 81X gene segment by inversion (black arrows) or to J H s gene segments by deletion (blue arrows), respectively (left). Products of each form of rearrangements are shown to the right. RAG2-deficient pro–B cell lines with WT or IGCR1-mutated IgH alleles [CBE −/− (1) and CBE −/− (2)] were infected with a Rag2-expressing lentivirus, followed by genomic DNA purification after 14 days of selection with puromycin. LAM-HTGTS experiments were carried out as previously described ( 33 , 39 ) with baits (red arrows) located 50- to 100-bp upstream of DQ52 (A) or DSP2 (B). Restriction enzyme Sacl-HF (R3156S, NEB) and BseYI (R0635S, NEB) were used to remove germline DNA with DQ52 and DSP2 as bait, respectively. Total reads were aligned to detect recombination by deletion to J H s and by inversion to V H 81X. The lower reads of 3′ DSP2 RSS utilization compared to DQ52 gene may be due to inefficient restriction of germline DSP2 fragments during library preparation. Average reads and percentages from two independent experiments are shown in red.

    Journal: Science Advances

    Article Title: Altered 3D chromatin structure permits inversional recombination at the IgH locus

    doi: 10.1126/sciadv.aaz8850

    Figure Lengend Snippet: Inversional and deletional recombination of 3′-RSS of DQ52 and DSP2 on WT and IGCR1-mutated IgH alleles. Schematic representation of 3′-RSS of DQ52 ( A ) and DSP2 ( B ) rearrangements to V H 81X gene segment by inversion (black arrows) or to J H s gene segments by deletion (blue arrows), respectively (left). Products of each form of rearrangements are shown to the right. RAG2-deficient pro–B cell lines with WT or IGCR1-mutated IgH alleles [CBE −/− (1) and CBE −/− (2)] were infected with a Rag2-expressing lentivirus, followed by genomic DNA purification after 14 days of selection with puromycin. LAM-HTGTS experiments were carried out as previously described ( 33 , 39 ) with baits (red arrows) located 50- to 100-bp upstream of DQ52 (A) or DSP2 (B). Restriction enzyme Sacl-HF (R3156S, NEB) and BseYI (R0635S, NEB) were used to remove germline DNA with DQ52 and DSP2 as bait, respectively. Total reads were aligned to detect recombination by deletion to J H s and by inversion to V H 81X. The lower reads of 3′ DSP2 RSS utilization compared to DQ52 gene may be due to inefficient restriction of germline DSP2 fragments during library preparation. Average reads and percentages from two independent experiments are shown in red.

    Article Snippet: Restriction enzyme Sac 1–HF [R3156S, New England Biolabs (NEB)] and Bse YI (R0635S, NEB) were used to digest germline DNA with DQ52 and DSP2 as bait, respectively.

    Techniques: Infection, Expressing, DNA Purification, Selection, Laser Capture Microdissection

    Map of PCR primer binding sites and restriction enzyme cut sizes for the barcode fragment of COI. BamHI restriction sites are only found in haplotype group A alleles while AflIII and BseYI restriction sites are only found in haplotype group B alleles.

    Journal: Systematic entomology

    Article Title: A tale of two haplotype groups: Evaluating the New World Junonia ring species hypothesis using the distribution of divergent COI haplotypes

    doi: 10.1111/syen.12120

    Figure Lengend Snippet: Map of PCR primer binding sites and restriction enzyme cut sizes for the barcode fragment of COI. BamHI restriction sites are only found in haplotype group A alleles while AflIII and BseYI restriction sites are only found in haplotype group B alleles.

    Article Snippet: PCR products of the correct size were evaluated by a triple digest using BseYI, AflIII, and BamHI restriction endonucleases (New England Biolabs, Ipswich, MA, USA) ( ).

    Techniques: Polymerase Chain Reaction, Binding Assay