alei  (New England Biolabs)


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  • 93
    Name:
    AleI
    Description:
    AleI 2 500 units
    Catalog Number:
    r0634l
    Price:
    272
    Size:
    2 500 units
    Category:
    DNA Manipulation
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    Structured Review

    New England Biolabs alei
    AleI
    AleI 2 500 units
    https://www.bioz.com/result/alei/product/New England Biolabs
    Average 93 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    alei - by Bioz Stars, 2020-07
    93/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Lack of Methylated Hopanoids Renders the Cyanobacterium Nostoc punctiforme Sensitive to Osmotic and pH Stress
    Article Snippet: .. A central portion of the cloned N. punctiforme hpnP gene, consisting of 1,113 bp, was removed by digestion with the restriction enzymes MscI and AleI (New England BioLabs [NEB]). .. The omega fragment containing the neomycin resistance gene ( npt ) and associated psbA promoter (PpsbA) of plasmid pSCR9 ( ) was isolated by SmaI (NEB) restriction digestion.

    Nucleic Acid Electrophoresis:

    Article Title: The MicroRNA, miR‐18a, Regulates NeuroD and Photoreceptor Differentiation in the Retina of Zebrafish
    Article Snippet: .. The same screening primers (above) were used for this method and AleI digest using standard protocols (New England Biolabs) cuts the mutant PCR product into 244 and 407 bp segments that are easily visualized using gel electrophoresis. .. F1 generation heterozygous adult fish were outcrossed and then F2 generation heterozygotes incrossed to produce a homozygous line of miR‐18a mutants, and these fish were incrossed to produce homozygous mutant embryos used here.

    Mutagenesis:

    Article Title: The MicroRNA, miR‐18a, Regulates NeuroD and Photoreceptor Differentiation in the Retina of Zebrafish
    Article Snippet: .. The same screening primers (above) were used for this method and AleI digest using standard protocols (New England Biolabs) cuts the mutant PCR product into 244 and 407 bp segments that are easily visualized using gel electrophoresis. .. F1 generation heterozygous adult fish were outcrossed and then F2 generation heterozygotes incrossed to produce a homozygous line of miR‐18a mutants, and these fish were incrossed to produce homozygous mutant embryos used here.

    Construct:

    Article Title: A Novel Approach for the Rapid Mutagenesis and Directed Evolution of the Structural Genes of West Nile Virus
    Article Snippet: .. The resulting construct encodes a stop codon in the capsid gene; downstream sequences of this insertion are no longer in frame. pWNV-complement-3′ was constructed by the cleavage of pWNV-complement with PshAI and AleI (removing nucleotides 1920 to 1933 of WNV NY99) (New England BioLabs), followed by intramolecular religation. .. This construct also encodes frameshifting to introduce a stop codon in the E gene; downstream flavivirus sequences in this construct are no longer in frame. pWNV-GFP-backbone was designed by using a configuration described previously ( ).

    Polymerase Chain Reaction:

    Article Title: The MicroRNA, miR‐18a, Regulates NeuroD and Photoreceptor Differentiation in the Retina of Zebrafish
    Article Snippet: .. The same screening primers (above) were used for this method and AleI digest using standard protocols (New England Biolabs) cuts the mutant PCR product into 244 and 407 bp segments that are easily visualized using gel electrophoresis. .. F1 generation heterozygous adult fish were outcrossed and then F2 generation heterozygotes incrossed to produce a homozygous line of miR‐18a mutants, and these fish were incrossed to produce homozygous mutant embryos used here.

    Plasmid Preparation:

    Article Title: No evidence for genome editing in mouse zygotes and HEK293T human cell line using the DNA-guided Natronobacterium gregoryi Argonaute (NgAgo)
    Article Snippet: .. Construction of a Flag-nls-NgAgo-GK plasmid The nls-NgAgo-GK plasmid was a gift from Chunyu Han (Addgene plasmid 78253). nls-NgAgo-GK plasmid was digested overnight with AleI (NEB, cat no. R0634S). .. Following the digestion a forward ( 5’-TGGACTATAAGGACCACGACGGAGACTACAAGGATCATGATATTGATTACAAAGACGATGACGATAAGA-3’ ) and a reverse ( 5’ -TCTTATCGTCATCGTCTTTGTAATCAATATCATGATCCTTGTAGTCTCCGTCGTGGTCCTTATAGTCCA-3’ ) oligonucleotide sequence encoding for a flag tag were annealed and ligated into the nls-NgAgo-GK plasmid upstream to the SV40 Nuclear Localization Signal (nls).

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  • 93
    New England Biolabs alei
    Alei, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/alei/product/New England Biolabs
    Average 93 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    alei - by Bioz Stars, 2020-07
    93/100 stars
      Buy from Supplier

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