bmgbi  (New England Biolabs)


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  • 93
    Name:
    BmgBI
    Description:
    BmgBI 2 500 units
    Catalog Number:
    R0628L
    Price:
    290
    Category:
    Restriction Enzymes
    Size:
    2 500 units
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    Structured Review

    New England Biolabs bmgbi
    BmgBI
    BmgBI 2 500 units
    https://www.bioz.com/result/bmgbi/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bmgbi - by Bioz Stars, 2021-05
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: A novel reporter for real-time, quantitative imaging of AKT-directed K63-poly-ubiquitination in living cells
    Article Snippet: A seven amino acid long poly Alanine linker shown to increase the specificity of the synthetic UBD to K63-linkage specific chains [ ] was inserted between the UBD and the AKT substrate peptide sequence. .. The BTR reporter [ ] was re-cloned into the plasmid harboring the above mentioned luciferase fragments and was opened with NotI-HF and XmaI and cipped using calf intestinal alkaline phosphatase (New England Biolabs). .. The PCR fragment containing the linkers, NZF domain, poly A linker, AKT substrate peptide was also digested with NotI-HF and XmaI and ligated using the Quick ligation kit (Roche) and transformed into XL-10 Gold cells (Invitrogen) and spread on agar plates containing ampicillin (50 µg/mL).

    Article Title: Herpes simplex viruses activate phospholipid scramblase to redistribute phosphatidylserines and Akt to the outer leaflet of the plasma membrane and promote viral entry
    Article Snippet: In select studies viral envelopes were labeled by incubating with a lipophilic tracer DiL (Invitrogen) for 10 min at room temperature before purification on sucrose gradients [ ]. .. Construction of a gL-2 deletion virus 79VB4 cells were co-transfected with HSV-2(G) DNA and DNA from an engineered plasmid (designated pEF6/5UL1HygroGFP) that contains the gL-2 gene (UL1) disrupted at the BmgBI and HpaI sites (R0628, R0105, New England BioLabs) by the hygromycin enhanced green fluorescent protein (EGFP) fusion protein under the control of the immediate early promoter of human cytomegalovirus (HCMV) constructed from pHygGFP (Clontech) (The plasmid encoding HSV-2(G) gL-2, designated pEF6/5 UL1 was a gift from Torin Weisbrod and William Jacobs).This dual function marker vector allows for drug selection with the ability to identify positive transfectants using GFP as a fluorescent reporter. .. Cotransfections were performed using the Effectene Transfection reagent (301425, Qiagen).

    Article Title: A High-Resolution View of Genome-Wide Pneumococcal Transformation
    Article Snippet: This fragment was purified through agarose gel electrophoresis using a QIAquick Gel Extraction kit (Qiagen). .. Plasmid pRight was similarly extracted, then digested with Bmg BI (New England Biolabs), which also cuts to give blunt ends. .. The ermB fragment was cloned into this blunt cut site using T4 ligase (Promega) overnight at 4°C according to manufacturer's instructions.

    Article Title: Herpes simplex viruses activate phospholipid scramblase to redistribute phosphatidylserines and Akt to the outer leaflet of the plasma membrane and promote viral entry
    Article Snippet: In select studies viral envelopes were labeled by incubating with a lipophilic tracer DiL (Invitrogen) for 10 min at room temperature before purification on sucrose gradients [ ]. .. 79VB4 cells were co-transfected with HSV-2(G) DNA and DNA from an engineered plasmid (designated pEF6/5UL1HygroGFP) that contains the gL-2 gene (UL1) disrupted at the BmgBI and HpaI sites (R0628, R0105, New England BioLabs) by the hygromycin enhanced green fluorescent protein (EGFP) fusion protein under the control of the immediate early promoter of human cytomegalovirus (HCMV) constructed from pHygGFP (Clontech) (The plasmid encoding HSV-2(G) gL-2, designated pEF6/5 UL1 was a gift from Torin Weisbrod and William Jacobs).This dual function marker vector allows for drug selection with the ability to identify positive transfectants using GFP as a fluorescent reporter. .. Cotran sfections were performed using the Effectene Transfection reagent (301425, Qiagen).

    Luciferase:

    Article Title: A novel reporter for real-time, quantitative imaging of AKT-directed K63-poly-ubiquitination in living cells
    Article Snippet: A seven amino acid long poly Alanine linker shown to increase the specificity of the synthetic UBD to K63-linkage specific chains [ ] was inserted between the UBD and the AKT substrate peptide sequence. .. The BTR reporter [ ] was re-cloned into the plasmid harboring the above mentioned luciferase fragments and was opened with NotI-HF and XmaI and cipped using calf intestinal alkaline phosphatase (New England Biolabs). .. The PCR fragment containing the linkers, NZF domain, poly A linker, AKT substrate peptide was also digested with NotI-HF and XmaI and ligated using the Quick ligation kit (Roche) and transformed into XL-10 Gold cells (Invitrogen) and spread on agar plates containing ampicillin (50 µg/mL).

    Polymerase Chain Reaction:

    Article Title: Association of A Novel Splice Site Mutation in P/Q-Type Calcium Channels with Childhood Epilepsy and Late-Onset Slowly Progressive Non-Episodic Cerebellar Ataxia
    Article Snippet: The primer sequences and PCR conditions are available upon request. .. The resulting PCR product was digested with Dra III and Bmg BI (both from New England Biolabs, Ipswich, MA, USA). .. After gel purification, the fragment was inserted into the corresponding restriction sites of the wild-type CACNA1A construct.

    Article Title: Novel Acidic Sesquiterpenoids Constitute a Dominant Class of Pathogen-Induced Phytoalexins in Maize 1Novel Acidic Sesquiterpenoids Constitute a Dominant Class of Pathogen-Induced Phytoalexins in Maize 1 [W]Novel Acidic Sesquiterpenoids Constitute a Dominant Class of Pathogen-Induced Phytoalexins in Maize 1 [W] [OA]
    Article Snippet: Specificity of real-time PCR products was verified on 1% agarose gels. .. To confirm the specificity of Tps6 and Tps11 primers, the PCR products of each primer pair were digested with BmgBI (New England Biolabs), which specifically digests the Tps6 amplicon but not Tps11. ..

    Article Title: Genetic Characterization of Second-Line Drug-Resistant and Extensively Drug-Resistant Mycobacterium tuberculosis from the Northern Region of India
    Article Snippet: For amplification of rrs , 200 μM of dNTPs (Fermentas, Lithuania), 20 pmol of forward primer RRSF, 5 pmol of internal reverse primer RRSR1, and 40 pmol of reverse primer RRSR were used. .. For PCR RFLP, the same primers RRSF and RRSR were used followed by the restriction digestion with BmgB I (New England Biolabs, UK). .. Fragments of 353 and 128 bp were obtained from PCR RFLP of rrs gene showing the wild-type genotype ( ).

    Construct:

    Article Title: Herpes simplex viruses activate phospholipid scramblase to redistribute phosphatidylserines and Akt to the outer leaflet of the plasma membrane and promote viral entry
    Article Snippet: In select studies viral envelopes were labeled by incubating with a lipophilic tracer DiL (Invitrogen) for 10 min at room temperature before purification on sucrose gradients [ ]. .. Construction of a gL-2 deletion virus 79VB4 cells were co-transfected with HSV-2(G) DNA and DNA from an engineered plasmid (designated pEF6/5UL1HygroGFP) that contains the gL-2 gene (UL1) disrupted at the BmgBI and HpaI sites (R0628, R0105, New England BioLabs) by the hygromycin enhanced green fluorescent protein (EGFP) fusion protein under the control of the immediate early promoter of human cytomegalovirus (HCMV) constructed from pHygGFP (Clontech) (The plasmid encoding HSV-2(G) gL-2, designated pEF6/5 UL1 was a gift from Torin Weisbrod and William Jacobs).This dual function marker vector allows for drug selection with the ability to identify positive transfectants using GFP as a fluorescent reporter. .. Cotransfections were performed using the Effectene Transfection reagent (301425, Qiagen).

    Article Title: Herpes simplex viruses activate phospholipid scramblase to redistribute phosphatidylserines and Akt to the outer leaflet of the plasma membrane and promote viral entry
    Article Snippet: In select studies viral envelopes were labeled by incubating with a lipophilic tracer DiL (Invitrogen) for 10 min at room temperature before purification on sucrose gradients [ ]. .. 79VB4 cells were co-transfected with HSV-2(G) DNA and DNA from an engineered plasmid (designated pEF6/5UL1HygroGFP) that contains the gL-2 gene (UL1) disrupted at the BmgBI and HpaI sites (R0628, R0105, New England BioLabs) by the hygromycin enhanced green fluorescent protein (EGFP) fusion protein under the control of the immediate early promoter of human cytomegalovirus (HCMV) constructed from pHygGFP (Clontech) (The plasmid encoding HSV-2(G) gL-2, designated pEF6/5 UL1 was a gift from Torin Weisbrod and William Jacobs).This dual function marker vector allows for drug selection with the ability to identify positive transfectants using GFP as a fluorescent reporter. .. Cotran sfections were performed using the Effectene Transfection reagent (301425, Qiagen).

    Marker:

    Article Title: Herpes simplex viruses activate phospholipid scramblase to redistribute phosphatidylserines and Akt to the outer leaflet of the plasma membrane and promote viral entry
    Article Snippet: In select studies viral envelopes were labeled by incubating with a lipophilic tracer DiL (Invitrogen) for 10 min at room temperature before purification on sucrose gradients [ ]. .. Construction of a gL-2 deletion virus 79VB4 cells were co-transfected with HSV-2(G) DNA and DNA from an engineered plasmid (designated pEF6/5UL1HygroGFP) that contains the gL-2 gene (UL1) disrupted at the BmgBI and HpaI sites (R0628, R0105, New England BioLabs) by the hygromycin enhanced green fluorescent protein (EGFP) fusion protein under the control of the immediate early promoter of human cytomegalovirus (HCMV) constructed from pHygGFP (Clontech) (The plasmid encoding HSV-2(G) gL-2, designated pEF6/5 UL1 was a gift from Torin Weisbrod and William Jacobs).This dual function marker vector allows for drug selection with the ability to identify positive transfectants using GFP as a fluorescent reporter. .. Cotransfections were performed using the Effectene Transfection reagent (301425, Qiagen).

    Article Title: Herpes simplex viruses activate phospholipid scramblase to redistribute phosphatidylserines and Akt to the outer leaflet of the plasma membrane and promote viral entry
    Article Snippet: In select studies viral envelopes were labeled by incubating with a lipophilic tracer DiL (Invitrogen) for 10 min at room temperature before purification on sucrose gradients [ ]. .. 79VB4 cells were co-transfected with HSV-2(G) DNA and DNA from an engineered plasmid (designated pEF6/5UL1HygroGFP) that contains the gL-2 gene (UL1) disrupted at the BmgBI and HpaI sites (R0628, R0105, New England BioLabs) by the hygromycin enhanced green fluorescent protein (EGFP) fusion protein under the control of the immediate early promoter of human cytomegalovirus (HCMV) constructed from pHygGFP (Clontech) (The plasmid encoding HSV-2(G) gL-2, designated pEF6/5 UL1 was a gift from Torin Weisbrod and William Jacobs).This dual function marker vector allows for drug selection with the ability to identify positive transfectants using GFP as a fluorescent reporter. .. Cotran sfections were performed using the Effectene Transfection reagent (301425, Qiagen).

    Selection:

    Article Title: Herpes simplex viruses activate phospholipid scramblase to redistribute phosphatidylserines and Akt to the outer leaflet of the plasma membrane and promote viral entry
    Article Snippet: In select studies viral envelopes were labeled by incubating with a lipophilic tracer DiL (Invitrogen) for 10 min at room temperature before purification on sucrose gradients [ ]. .. Construction of a gL-2 deletion virus 79VB4 cells were co-transfected with HSV-2(G) DNA and DNA from an engineered plasmid (designated pEF6/5UL1HygroGFP) that contains the gL-2 gene (UL1) disrupted at the BmgBI and HpaI sites (R0628, R0105, New England BioLabs) by the hygromycin enhanced green fluorescent protein (EGFP) fusion protein under the control of the immediate early promoter of human cytomegalovirus (HCMV) constructed from pHygGFP (Clontech) (The plasmid encoding HSV-2(G) gL-2, designated pEF6/5 UL1 was a gift from Torin Weisbrod and William Jacobs).This dual function marker vector allows for drug selection with the ability to identify positive transfectants using GFP as a fluorescent reporter. .. Cotransfections were performed using the Effectene Transfection reagent (301425, Qiagen).

    Article Title: Herpes simplex viruses activate phospholipid scramblase to redistribute phosphatidylserines and Akt to the outer leaflet of the plasma membrane and promote viral entry
    Article Snippet: In select studies viral envelopes were labeled by incubating with a lipophilic tracer DiL (Invitrogen) for 10 min at room temperature before purification on sucrose gradients [ ]. .. 79VB4 cells were co-transfected with HSV-2(G) DNA and DNA from an engineered plasmid (designated pEF6/5UL1HygroGFP) that contains the gL-2 gene (UL1) disrupted at the BmgBI and HpaI sites (R0628, R0105, New England BioLabs) by the hygromycin enhanced green fluorescent protein (EGFP) fusion protein under the control of the immediate early promoter of human cytomegalovirus (HCMV) constructed from pHygGFP (Clontech) (The plasmid encoding HSV-2(G) gL-2, designated pEF6/5 UL1 was a gift from Torin Weisbrod and William Jacobs).This dual function marker vector allows for drug selection with the ability to identify positive transfectants using GFP as a fluorescent reporter. .. Cotran sfections were performed using the Effectene Transfection reagent (301425, Qiagen).

    Amplification:

    Article Title: Novel Acidic Sesquiterpenoids Constitute a Dominant Class of Pathogen-Induced Phytoalexins in Maize 1Novel Acidic Sesquiterpenoids Constitute a Dominant Class of Pathogen-Induced Phytoalexins in Maize 1 [W]Novel Acidic Sesquiterpenoids Constitute a Dominant Class of Pathogen-Induced Phytoalexins in Maize 1 [W] [OA]
    Article Snippet: Specificity of real-time PCR products was verified on 1% agarose gels. .. To confirm the specificity of Tps6 and Tps11 primers, the PCR products of each primer pair were digested with BmgBI (New England Biolabs), which specifically digests the Tps6 amplicon but not Tps11. ..

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  • 93
    New England Biolabs bmgb i
    PCR RFLP of rrs gene after digestion with <t>BmgB</t> I. Lane 1, 100 bp DNA ladder; Lanes 2 and 3, 353 and 128 bp fragments with wild-type sequence; and Lanes 4 and 5, 481 bp fragments of mutant sequence
    Bmgb I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bmgb i/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bmgb i - by Bioz Stars, 2021-05
    93/100 stars
      Buy from Supplier

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    PCR RFLP of rrs gene after digestion with BmgB I. Lane 1, 100 bp DNA ladder; Lanes 2 and 3, 353 and 128 bp fragments with wild-type sequence; and Lanes 4 and 5, 481 bp fragments of mutant sequence

    Journal: Journal of Epidemiology and Global Health

    Article Title: Genetic Characterization of Second-Line Drug-Resistant and Extensively Drug-Resistant Mycobacterium tuberculosis from the Northern Region of India

    doi: 10.2991/j.jegh.2018.02.100

    Figure Lengend Snippet: PCR RFLP of rrs gene after digestion with BmgB I. Lane 1, 100 bp DNA ladder; Lanes 2 and 3, 353 and 128 bp fragments with wild-type sequence; and Lanes 4 and 5, 481 bp fragments of mutant sequence

    Article Snippet: For PCR RFLP, the same primers RRSF and RRSR were used followed by the restriction digestion with BmgB I (New England Biolabs, UK).

    Techniques: Polymerase Chain Reaction, Sequencing, Mutagenesis