bce ai  (New England Biolabs)


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    Name:
    BceAI
    Description:
    BceAI 250 units
    Catalog Number:
    r0623l
    Price:
    269
    Size:
    250 units
    Category:
    Restriction Enzymes
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    Structured Review

    New England Biolabs bce ai
    BceAI
    BceAI 250 units
    https://www.bioz.com/result/bce ai/product/New England Biolabs
    Average 93 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    bce ai - by Bioz Stars, 2020-09
    93/100 stars

    Images

    1) Product Images from "PCR-Restriction Fragment Length Polymorphism Can Also Detect Point Mutation A2142C in the 23S rRNA Gene, Associated with Helicobacter pylori Resistance to Clarithromycin"

    Article Title: PCR-Restriction Fragment Length Polymorphism Can Also Detect Point Mutation A2142C in the 23S rRNA Gene, Associated with Helicobacter pylori Resistance to Clarithromycin

    Journal: Antimicrobial Agents and Chemotherapy

    doi: 10.1128/AAC.46.4.1156-1157.2002

    Detection of mutation A2142C by Bce AI-mediated restriction digestion. The restriction fragments of the 267-bp PCR products were analyzed by electrophoresis on a 5% agarose Resophor gel (A) or on a 12% polyacrylamide gel (B) stained with ethidium bromide. (A) PCR-RFLP analysis of mutations A2142G, A2143G, and A2142C occurring in domain V of the 23S rRNA gene of H. pylori . Lanes 1 and 8, 25-bp DNA Step Ladder molecular size markers (Promega). Lanes 2 and 3, PCR products of the wild-type and A2142G H. pylori strains digested with Bbs I, respectively. Lanes 4 and 5, PCR products of the wild-type and A2143G H. pylori strains digested with Bsa I, respectively. Lanes 6 and 7, PCR products of the wild-type and A2142C H. pylori strains digested with Bce AI, respectively. (B) PCR product of the H. pylori strain with mutation A2142C digested with Bce AI. Lanes 2 and 3, amplified wild-type PCR product and amplified PCR product presenting the A2142C mutation, respectively. Lane 1, 25-bp DNA Step Ladder (Promega). The wild-type H. pylori ).
    Figure Legend Snippet: Detection of mutation A2142C by Bce AI-mediated restriction digestion. The restriction fragments of the 267-bp PCR products were analyzed by electrophoresis on a 5% agarose Resophor gel (A) or on a 12% polyacrylamide gel (B) stained with ethidium bromide. (A) PCR-RFLP analysis of mutations A2142G, A2143G, and A2142C occurring in domain V of the 23S rRNA gene of H. pylori . Lanes 1 and 8, 25-bp DNA Step Ladder molecular size markers (Promega). Lanes 2 and 3, PCR products of the wild-type and A2142G H. pylori strains digested with Bbs I, respectively. Lanes 4 and 5, PCR products of the wild-type and A2143G H. pylori strains digested with Bsa I, respectively. Lanes 6 and 7, PCR products of the wild-type and A2142C H. pylori strains digested with Bce AI, respectively. (B) PCR product of the H. pylori strain with mutation A2142C digested with Bce AI. Lanes 2 and 3, amplified wild-type PCR product and amplified PCR product presenting the A2142C mutation, respectively. Lane 1, 25-bp DNA Step Ladder (Promega). The wild-type H. pylori ).

    Techniques Used: Mutagenesis, Polymerase Chain Reaction, Electrophoresis, Staining, Amplification

    2) Product Images from "PCR-Restriction Fragment Length Polymorphism Analysis for Detection of Point Mutations Associated with Macrolide Resistance in Campylobacter spp."

    Article Title: PCR-Restriction Fragment Length Polymorphism Analysis for Detection of Point Mutations Associated with Macrolide Resistance in Campylobacter spp.

    Journal: Antimicrobial Agents and Chemotherapy

    doi: 10.1128/AAC.47.3.1125-1128.2003

    PCR-restriction fragment length polymorphism patterns obtained after digestion with Bsa I and Bce AI. The restriction products were analyzed by electrophoresis on a 10% polyacrylamide gel stained with ethidium bromide. Wild-type C. jejuni reference strain NCTC 11168 and strains 00072, 01206, and 00039, with mutations A2075G, A2074C, and both A2074C and A2075G occurring in domain V of the 23S rRNA gene, were used. Lanes: M, 25-bp DNA Step Ladder molecular size markers (Promega); 1, nondigested PCR products of wild-type C. jejuni strain NCTC 11168; 2 to 5, Bsa I-digested PCR products of the wild-type and A2075G, A2074C, and A2074C-A2075G mutant C. jejuni strains, respectively; 6 to 9, Bce AI-digested PCR products of the wild-type, A2075G, A2074C, and A2074C-A2075G C. jejuni strains, respectively.
    Figure Legend Snippet: PCR-restriction fragment length polymorphism patterns obtained after digestion with Bsa I and Bce AI. The restriction products were analyzed by electrophoresis on a 10% polyacrylamide gel stained with ethidium bromide. Wild-type C. jejuni reference strain NCTC 11168 and strains 00072, 01206, and 00039, with mutations A2075G, A2074C, and both A2074C and A2075G occurring in domain V of the 23S rRNA gene, were used. Lanes: M, 25-bp DNA Step Ladder molecular size markers (Promega); 1, nondigested PCR products of wild-type C. jejuni strain NCTC 11168; 2 to 5, Bsa I-digested PCR products of the wild-type and A2075G, A2074C, and A2074C-A2075G mutant C. jejuni strains, respectively; 6 to 9, Bce AI-digested PCR products of the wild-type, A2075G, A2074C, and A2074C-A2075G C. jejuni strains, respectively.

    Techniques Used: Polymerase Chain Reaction, Electrophoresis, Staining, Mutagenesis

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Absence of the mutated Trp2 allele but a common polymorphism of the COL9A2 collagen gene is associated with early recurrence after lumbar discectomy in a German population
    Article Snippet: .. Therefore, purified PCR fragments from 20 μl of the initial PCR were digested in a 25 μl reaction containing 2 U Bce AI (New England Biolabs, Beverly, MA), 2 μg BSA at 37°C for 12 h. For details see Tables . .. Results were confirmed by direct sequencing of the PCR results of four different probes.

    Article Title: PCR-Restriction Fragment Length Polymorphism Analysis for Detection of Point Mutations Associated with Macrolide Resistance in Campylobacter spp.
    Article Snippet: .. After successful amplification of the 316-bp PCR products, amplicons were precipitated and suspended in 15 μl of H2 O and 5 μl of the amplicons was digested with the restriction enzymes Bsa I (5 U) and Bce AI (1 U) (New England Biolabs, Beverly, Mass.) as previously reported for H. pylori ( ). .. The fragments were incubated overnight at 50°C for Bsa I and 37°C for Bce AI, separated by electrophoresis on a 10% acrylamide gel, and visualized under UV light after ethidium bromide staining.

    Article Title: Clarithromycin resistance and prevalence of Helicobacter pylori virulent genotypes in patients from Southern México with chronic gastritis.
    Article Snippet: .. PCR products were digested by the restriction enzymes, BbsI ([GAAGAC] 59 and 28 bp) for the A2142G mutation, BceAI ([ACGGC] 64 and 23 bp) for the A2142C mutation and BsaI for the A2143G mutation ([GGTCTC] 40 and 47 bp) (BioLabs Inc., New England), (Fig. 1). .. To refine the search of point mutations in the 23S rRNA gene, qPCR (real time PCR) was performed using primers mentioned previously and hydrolysis probes (De Francesco et al., 2009) listed in Table 1.

    Article Title: PCR-Restriction Fragment Length Polymorphism Can Also Detect Point Mutation A2142C in the 23S rRNA Gene, Associated with Helicobacter pylori Resistance to Clarithromycin
    Article Snippet: .. The 267-bp PCR products were precipitated and suspended in 15 μl of H2 O, and 5 μl was digested overnight in a final volume of 15 μl with the restriction enzymes Bbs I (5 U), Bsa I (5 U) , and Bce AI (0.5 U) (New England Biolabs). .. PCR-RFLP allowed the identification of mutations A2142G and A2143G using the Bbs I and Bsa I restriction enzymes, respectively (Fig. , lanes 3 and 5), as previously described ( , , ).

    Mutagenesis:

    Article Title: Clarithromycin resistance and prevalence of Helicobacter pylori virulent genotypes in patients from Southern México with chronic gastritis.
    Article Snippet: .. PCR products were digested by the restriction enzymes, BbsI ([GAAGAC] 59 and 28 bp) for the A2142G mutation, BceAI ([ACGGC] 64 and 23 bp) for the A2142C mutation and BsaI for the A2143G mutation ([GGTCTC] 40 and 47 bp) (BioLabs Inc., New England), (Fig. 1). .. To refine the search of point mutations in the 23S rRNA gene, qPCR (real time PCR) was performed using primers mentioned previously and hydrolysis probes (De Francesco et al., 2009) listed in Table 1.

    Amplification:

    Article Title: PCR-Restriction Fragment Length Polymorphism Analysis for Detection of Point Mutations Associated with Macrolide Resistance in Campylobacter spp.
    Article Snippet: .. After successful amplification of the 316-bp PCR products, amplicons were precipitated and suspended in 15 μl of H2 O and 5 μl of the amplicons was digested with the restriction enzymes Bsa I (5 U) and Bce AI (1 U) (New England Biolabs, Beverly, Mass.) as previously reported for H. pylori ( ). .. The fragments were incubated overnight at 50°C for Bsa I and 37°C for Bce AI, separated by electrophoresis on a 10% acrylamide gel, and visualized under UV light after ethidium bromide staining.

    Article Title: The naked truth: Sphynx and Devon Rex cat breed mutations in KRT71
    Article Snippet: .. Approximately 20 μl of amplified product was digested with 2 U of BceAI (New England Biolabs Inc., Beverly, MA) at 37°C overnight followed by inactivation of the enzyme at 65°C for 10 min. .. The primers generated an amplification product of 832 bp that produced 436- and 396-bp restriction fragment products using BceAI when the G/A SNP was absent.

    Purification:

    Article Title: Absence of the mutated Trp2 allele but a common polymorphism of the COL9A2 collagen gene is associated with early recurrence after lumbar discectomy in a German population
    Article Snippet: .. Therefore, purified PCR fragments from 20 μl of the initial PCR were digested in a 25 μl reaction containing 2 U Bce AI (New England Biolabs, Beverly, MA), 2 μg BSA at 37°C for 12 h. For details see Tables . .. Results were confirmed by direct sequencing of the PCR results of four different probes.

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    New England Biolabs bce ai
    Detection of mutation A2142C by <t>Bce</t> AI-mediated restriction digestion. The restriction fragments of the 267-bp PCR products were analyzed by electrophoresis on a 5% agarose Resophor gel (A) or on a 12% polyacrylamide gel (B) stained with ethidium bromide. (A) PCR-RFLP analysis of mutations A2142G, A2143G, and A2142C occurring in domain V of the 23S rRNA gene of H. pylori . Lanes 1 and 8, 25-bp DNA Step Ladder molecular size markers (Promega). Lanes 2 and 3, PCR products of the wild-type and A2142G H. pylori strains digested with Bbs I, respectively. Lanes 4 and 5, PCR products of the wild-type and A2143G H. pylori strains digested with <t>Bsa</t> I, respectively. Lanes 6 and 7, PCR products of the wild-type and A2142C H. pylori strains digested with Bce AI, respectively. (B) PCR product of the H. pylori strain with mutation A2142C digested with Bce AI. Lanes 2 and 3, amplified wild-type PCR product and amplified PCR product presenting the A2142C mutation, respectively. Lane 1, 25-bp DNA Step Ladder (Promega). The wild-type H. pylori ).
    Bce Ai, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bce ai/product/New England Biolabs
    Average 93 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    bce ai - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

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    Detection of mutation A2142C by Bce AI-mediated restriction digestion. The restriction fragments of the 267-bp PCR products were analyzed by electrophoresis on a 5% agarose Resophor gel (A) or on a 12% polyacrylamide gel (B) stained with ethidium bromide. (A) PCR-RFLP analysis of mutations A2142G, A2143G, and A2142C occurring in domain V of the 23S rRNA gene of H. pylori . Lanes 1 and 8, 25-bp DNA Step Ladder molecular size markers (Promega). Lanes 2 and 3, PCR products of the wild-type and A2142G H. pylori strains digested with Bbs I, respectively. Lanes 4 and 5, PCR products of the wild-type and A2143G H. pylori strains digested with Bsa I, respectively. Lanes 6 and 7, PCR products of the wild-type and A2142C H. pylori strains digested with Bce AI, respectively. (B) PCR product of the H. pylori strain with mutation A2142C digested with Bce AI. Lanes 2 and 3, amplified wild-type PCR product and amplified PCR product presenting the A2142C mutation, respectively. Lane 1, 25-bp DNA Step Ladder (Promega). The wild-type H. pylori ).

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: PCR-Restriction Fragment Length Polymorphism Can Also Detect Point Mutation A2142C in the 23S rRNA Gene, Associated with Helicobacter pylori Resistance to Clarithromycin

    doi: 10.1128/AAC.46.4.1156-1157.2002

    Figure Lengend Snippet: Detection of mutation A2142C by Bce AI-mediated restriction digestion. The restriction fragments of the 267-bp PCR products were analyzed by electrophoresis on a 5% agarose Resophor gel (A) or on a 12% polyacrylamide gel (B) stained with ethidium bromide. (A) PCR-RFLP analysis of mutations A2142G, A2143G, and A2142C occurring in domain V of the 23S rRNA gene of H. pylori . Lanes 1 and 8, 25-bp DNA Step Ladder molecular size markers (Promega). Lanes 2 and 3, PCR products of the wild-type and A2142G H. pylori strains digested with Bbs I, respectively. Lanes 4 and 5, PCR products of the wild-type and A2143G H. pylori strains digested with Bsa I, respectively. Lanes 6 and 7, PCR products of the wild-type and A2142C H. pylori strains digested with Bce AI, respectively. (B) PCR product of the H. pylori strain with mutation A2142C digested with Bce AI. Lanes 2 and 3, amplified wild-type PCR product and amplified PCR product presenting the A2142C mutation, respectively. Lane 1, 25-bp DNA Step Ladder (Promega). The wild-type H. pylori ).

    Article Snippet: The 267-bp PCR products were precipitated and suspended in 15 μl of H2 O, and 5 μl was digested overnight in a final volume of 15 μl with the restriction enzymes Bbs I (5 U), Bsa I (5 U) , and Bce AI (0.5 U) (New England Biolabs).

    Techniques: Mutagenesis, Polymerase Chain Reaction, Electrophoresis, Staining, Amplification

    PCR-restriction fragment length polymorphism patterns obtained after digestion with Bsa I and Bce AI. The restriction products were analyzed by electrophoresis on a 10% polyacrylamide gel stained with ethidium bromide. Wild-type C. jejuni reference strain NCTC 11168 and strains 00072, 01206, and 00039, with mutations A2075G, A2074C, and both A2074C and A2075G occurring in domain V of the 23S rRNA gene, were used. Lanes: M, 25-bp DNA Step Ladder molecular size markers (Promega); 1, nondigested PCR products of wild-type C. jejuni strain NCTC 11168; 2 to 5, Bsa I-digested PCR products of the wild-type and A2075G, A2074C, and A2074C-A2075G mutant C. jejuni strains, respectively; 6 to 9, Bce AI-digested PCR products of the wild-type, A2075G, A2074C, and A2074C-A2075G C. jejuni strains, respectively.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: PCR-Restriction Fragment Length Polymorphism Analysis for Detection of Point Mutations Associated with Macrolide Resistance in Campylobacter spp.

    doi: 10.1128/AAC.47.3.1125-1128.2003

    Figure Lengend Snippet: PCR-restriction fragment length polymorphism patterns obtained after digestion with Bsa I and Bce AI. The restriction products were analyzed by electrophoresis on a 10% polyacrylamide gel stained with ethidium bromide. Wild-type C. jejuni reference strain NCTC 11168 and strains 00072, 01206, and 00039, with mutations A2075G, A2074C, and both A2074C and A2075G occurring in domain V of the 23S rRNA gene, were used. Lanes: M, 25-bp DNA Step Ladder molecular size markers (Promega); 1, nondigested PCR products of wild-type C. jejuni strain NCTC 11168; 2 to 5, Bsa I-digested PCR products of the wild-type and A2075G, A2074C, and A2074C-A2075G mutant C. jejuni strains, respectively; 6 to 9, Bce AI-digested PCR products of the wild-type, A2075G, A2074C, and A2074C-A2075G C. jejuni strains, respectively.

    Article Snippet: After successful amplification of the 316-bp PCR products, amplicons were precipitated and suspended in 15 μl of H2 O and 5 μl of the amplicons was digested with the restriction enzymes Bsa I (5 U) and Bce AI (1 U) (New England Biolabs, Beverly, Mass.) as previously reported for H. pylori ( ).

    Techniques: Polymerase Chain Reaction, Electrophoresis, Staining, Mutagenesis