hpy188 iii  (New England Biolabs)


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    Name:
    Hpy188III
    Description:
    Hpy188III 2 500 units
    Catalog Number:
    R0622L
    Price:
    277
    Size:
    2 500 units
    Category:
    Restriction Enzymes
    Score:
    85
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    Structured Review

    New England Biolabs hpy188 iii
    Hpy188III
    Hpy188III 2 500 units
    https://www.bioz.com/result/hpy188 iii/product/New England Biolabs
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hpy188 iii - by Bioz Stars, 2019-12
    98/100 stars

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    Related Articles

    Methylation Sequencing:

    Article Title: Demethylation of promoter C region of estrogen receptor ? gene is correlated with its enhanced expression in estrogen-ablation resistant MCF-7 cells
    Article Snippet: Paragraph title: 2.7. Bisulfite-sequencing and combined bisulfite restriction analysis (COBRA) ... For COBRA, 5 μl of purified DNA fragment was digested in a total volume of 20 μl using 5 units of Hpy 188III restriction enzyme (New England BioLabs, Ipswich, MA) that cleave CpG sites retained because of methylation at 37 °C for 4 h. The resultant DNA fragments together with undigested ones were electrophoresed onto 8% polyacrylamide gel.

    Clone Assay:

    Article Title: Sema3d controls collective endothelial cell migration by distinct mechanisms via Nrp1 and PlxnD1
    Article Snippet: Annealed CRISPR target site primers (CRIsema3d SE, 5′-TAGGAGACAGGTCAGGACGGGA-3′, and AS, 5′-AAACTCCCGTCCTGACCTGTCT-3′) were cloned into the guide RNA (gRNA) expression vector pDR274 as previously described ( ). .. 2 nl of sema3d gRNA (12.5 ng/µl) and nls-zCas9-nls -mRNA (300 ng/µl) were injected into one-cell-stage zebrafish embryos. sema3d mutant genotypes were analyzed by PCR using sema3d geno SE, 5′-CTGCCGAAGCAAATCTCCTA-3′, and AS, 5′-ATATGCCGCACATCCCCTAT-3′, followed by restriction digestion with Hpy188III (New England Biolabs, Inc.).

    Article Title: Human PTRF mutations cause secondary deficiency of caveolins resulting in muscular dystrophy with generalized lipodystrophy
    Article Snippet: In order to determine the frequency of the mutations in PTRF , we performed enzyme digestion of PCR products from 200 Japanese control subjects using Hpy 188III (New England Biolabs) for c.696_697insC and Taq I (New England Biolabs) for c.525delG. .. In order to determine the frequency of the mutations in PTRF , we performed enzyme digestion of PCR products from 200 Japanese control subjects using Hpy 188III (New England Biolabs) for c.696_697insC and Taq I (New England Biolabs) for c.525delG.

    Amplification:

    Article Title: Association of toll-like receptors polymorphism and intrauterine transmission of cytomegalovirus
    Article Snippet: The PCR amplification method was as follows: 3 min at 94°C followed by 40 cycles each of 30 seconds of denaturation at 95°C, 30 seconds annealing (rs4696480 at 67°C, rs3804100 at 60°C and rs179008 at 65°C) and 20 seconds elongation at 72°C. .. PCR products were digested for 1 hour at 37°C with Hpy188III (New England Biolabs, USA) for rs4696480, with HpyCH4III (New England Biolabs, USA) for rs3804100 and with ApoI-Fast Digest (Thermo Scientific, USA) for rs179008.

    Article Title: Application of Oocyte Cryopreservation Technology in TALEN-Mediated Mouse Genome Editing
    Article Snippet: Genomic PCR for amplification of theClec4b1 gene was performed under the following conditions: 94°C for 2 min; followed by 35 cycles of 94°C for 30 s, 59°C for 30 s, and 72°C for 20 s. The PCR primers for the Clec4b1 gene were 5′-GGCTATCTCTGTGGTATTTAGCTC-3′ and 5′-CCACTTGTCTGCATGGTTAC-3′. .. Each sample was then digested at 37°C overnight in 10 µ l of digestion solution containing 1×CutSmart buffer and 1.25 U of Hph I (New England Biolabs, Tokyo, Japan) or Hpy 188III (New England Biolabs) for the bL andClec4b1 genes, respectively.

    Article Title: FSH dystrophy and a subtelomeric 4q haplotype: a new assay and associations with disease
    Article Snippet: Differential digestion at SNP3 should distinguish the 4qA161 allele from the other reported haplotypes of 4q and 10q ( ) based upon sequencing of at least three alleles per haplotype by Lemmers et al . .. We amplified a sequence that contains SNP3 from somatic cell hybrid (SCH) and genomic DNAs and digested aliquots of the PCR products with Hpy188I or Hpy188III. .. SCH DNAs were completely sensitive to Hpy188I and resistant to Hpy188III or vice versa, as expected ( and data not shown).

    Article Title: FSH dystrophy and a subtelomeric 4q haplotype: a new assay and associations with disease
    Article Snippet: Genomic DNA (100 ng) was amplified in a 25-µl volume using 0.2 µM primers 1-F (5′-CTGTGGTCATCTCTGCTCCA-3′) and 1-R (5′-TTTGCCTGTGAGTTCGAATG-3′) as follows: 95°C, 2 min; then 45 cycles of 95°C, 20 s; 60°C, 20 s; 72°C, 20 s; followed by 72°C for 5 min. For each set of PCRs, a no-template control was the last sample. .. Aliquots of the single 168-bp PCR product (10 µl) were digested with 20 U of Hpy188I or of Hpy188III (New England Biolabs) for 3 h at 37°C (40 µl reaction volume).

    Article Title: Human PTRF mutations cause secondary deficiency of caveolins resulting in muscular dystrophy with generalized lipodystrophy
    Article Snippet: In order to determine the frequency of the mutations in PTRF , we performed enzyme digestion of PCR products from 200 Japanese control subjects using Hpy 188III (New England Biolabs) for c.696_697insC and Taq I (New England Biolabs) for c.525delG. .. PCR products were analyzed by direct sequencing or enzyme digestion using Mae III (Boehringer Mannheim).

    Article Title: POWERDRESS and Diversified Expression of the MIR172 Gene Family Bolster the Floral Stem Cell Network
    Article Snippet: For miR172d-1 , the primers R194geno-F and R194geno-R were used to amplify the genomic fragment. .. The fragment amplified from miR172d-1 fails to be cut by Hpy188 III (NEB, Cat# R0622). .. To generate pPWR:PWR-GFP , the PWR genomic region was amplified using primers EN1_full_CACC and EN1cDNA_NS ( ).

    Article Title: Friedreich Ataxia Patient Tissues Exhibit Increased 5-Hydroxymethylcytosine Modification and Decreased CTCF Binding at the FXN Locus
    Article Snippet: The MSREs used for CpGs 3, 6, 11 and 13 were Aci I (Fermentas), Hpy 188III (New England Biolabs), Aji I (Fermentas) and Eco 72I (Fermentas), respectively. .. The MSREs used for CpGs 3, 6, 11 and 13 were Aci I (Fermentas), Hpy 188III (New England Biolabs), Aji I (Fermentas) and Eco 72I (Fermentas), respectively.

    Article Title: Generation and Characterisation of Friedreich Ataxia YG8R Mouse Fibroblast and Neural Stem Cell Models
    Article Snippet: The MSREs used for CpGs 3 and 6 were AciI (Fermentas), and Hpy188III (New England Biolabs), respectively . .. The MSREs used for CpGs 3 and 6 were AciI (Fermentas), and Hpy188III (New England Biolabs), respectively .

    Article Title: Association of the Serotonin Receptor 3E Gene as a Functional Variant in the MicroRNA-510 Target Site with Diarrhea Predominant Irritable Bowel Syndrome in Chinese Women
    Article Snippet: Genotyping was performed using polymerase chain reaction (PCR) amplification and restriction fragment length polymorphism (RFLP) analyses. .. Briefly, 3 μL of the PCR product of HTR3E was digested with 0.5 μL of the restriction enzyme Hpy188III (New England Biolabs, Ipswich, MA, USA), according to the manufacturer’s recommendations.

    Article Title: Demethylation of promoter C region of estrogen receptor ? gene is correlated with its enhanced expression in estrogen-ablation resistant MCF-7 cells
    Article Snippet: The amplified fragments were purified using NucleoSpin Extract II kit (MACHEREY-NAGEL). .. For COBRA, 5 μl of purified DNA fragment was digested in a total volume of 20 μl using 5 units of Hpy 188III restriction enzyme (New England BioLabs, Ipswich, MA) that cleave CpG sites retained because of methylation at 37 °C for 4 h. The resultant DNA fragments together with undigested ones were electrophoresed onto 8% polyacrylamide gel.

    Synthesized:

    Article Title: Sema3d controls collective endothelial cell migration by distinct mechanisms via Nrp1 and PlxnD1
    Article Snippet: Nls-zCas9-nls mRNA was synthesized as previously described ( ). .. 2 nl of sema3d gRNA (12.5 ng/µl) and nls-zCas9-nls -mRNA (300 ng/µl) were injected into one-cell-stage zebrafish embryos. sema3d mutant genotypes were analyzed by PCR using sema3d geno SE, 5′-CTGCCGAAGCAAATCTCCTA-3′, and AS, 5′-ATATGCCGCACATCCCCTAT-3′, followed by restriction digestion with Hpy188III (New England Biolabs, Inc.).

    Quantitative RT-PCR:

    Article Title: Generation and Characterisation of Friedreich Ataxia YG8R Mouse Fibroblast and Neural Stem Cell Models
    Article Snippet: The MSREs used for CpGs 3 and 6 were AciI (Fermentas), and Hpy188III (New England Biolabs), respectively . .. PCR quantification was carried out using the ΔCt method (values were calculated as 2ΔCt (mock – digest) with the mock value set at 100%) and RQ Manager software (Applied Biosystems).

    SYBR Green Assay:

    Article Title: Friedreich Ataxia Patient Tissues Exhibit Increased 5-Hydroxymethylcytosine Modification and Decreased CTCF Binding at the FXN Locus
    Article Snippet: The MSREs used for CpGs 3, 6, 11 and 13 were Aci I (Fermentas), Hpy 188III (New England Biolabs), Aji I (Fermentas) and Eco 72I (Fermentas), respectively. .. The MSREs used for CpGs 3, 6, 11 and 13 were Aci I (Fermentas), Hpy 188III (New England Biolabs), Aji I (Fermentas) and Eco 72I (Fermentas), respectively.

    Article Title: Generation and Characterisation of Friedreich Ataxia YG8R Mouse Fibroblast and Neural Stem Cell Models
    Article Snippet: The MSREs used for CpGs 3 and 6 were AciI (Fermentas), and Hpy188III (New England Biolabs), respectively . .. The MSREs used for CpGs 3 and 6 were AciI (Fermentas), and Hpy188III (New England Biolabs), respectively .

    Article Title: Polymorphisms in gyrA and gyrB Genes among Mycobacterium avium subsp. paratuberculosis Type I, II, and III Isolates
    Article Snippet: The 897-bp PCR products were submitted to restriction analysis with the enzyme Hpy188-III (New England Biolabs). .. Digestion was performed with 10 μl of PCR products, 5 U of enzyme, 1× NE buffer 4, and 0.5 μl of 100 μg/ml bovine serum albumin.

    Incubation:

    Article Title: FSH dystrophy and a subtelomeric 4q haplotype: a new assay and associations with disease
    Article Snippet: Aliquots of the single 168-bp PCR product (10 µl) were digested with 20 U of Hpy188I or of Hpy188III (New England Biolabs) for 3 h at 37°C (40 µl reaction volume). .. For Hpy188III, first 10 U was added, followed by 5 U after 1 and again after 2 h. Digests were electrophoresed in a 2.5% or 3% agarose gel for 3 h, followed by ethidium bromide staining (1 µg/ml) for 30 min. For about half of the reactions, 0.1 µg of a pUC19 plasmid DNA was added as an internal control to an aliquot of the digest and incubated concurrently. .. Upon electrophoresis, the internal controls showed complete digestion relative to plasmid DNA in the absence of PCR product.

    Article Title: Association of the Serotonin Receptor 3E Gene as a Functional Variant in the MicroRNA-510 Target Site with Diarrhea Predominant Irritable Bowel Syndrome in Chinese Women
    Article Snippet: PCR reactions were performed using a PTC-200 (MJ Research, Minnesota, USA) or a Mastercycler gradient thermal cycler (MJ Research) with the following protocols: incubation at 94°C for 2 minutes; 5 cycles of 94°C for 30 seconds, 68°C for 30 seconds, and 72°C for 30 seconds; 5 cycles of 94°C for 30 seconds, 64°C for 30 seconds, and 72°C for 30 seconds; 25 cycles of 94°C for 30 seconds, 60°C for 30 seconds, and 72°C for 30 seconds; and a final extension step of 72°C for 5 minutes. .. Briefly, 3 μL of the PCR product of HTR3E was digested with 0.5 μL of the restriction enzyme Hpy188III (New England Biolabs, Ipswich, MA, USA), according to the manufacturer’s recommendations.

    Expressing:

    Article Title: Sema3d controls collective endothelial cell migration by distinct mechanisms via Nrp1 and PlxnD1
    Article Snippet: Annealed CRISPR target site primers (CRIsema3d SE, 5′-TAGGAGACAGGTCAGGACGGGA-3′, and AS, 5′-AAACTCCCGTCCTGACCTGTCT-3′) were cloned into the guide RNA (gRNA) expression vector pDR274 as previously described ( ). .. 2 nl of sema3d gRNA (12.5 ng/µl) and nls-zCas9-nls -mRNA (300 ng/µl) were injected into one-cell-stage zebrafish embryos. sema3d mutant genotypes were analyzed by PCR using sema3d geno SE, 5′-CTGCCGAAGCAAATCTCCTA-3′, and AS, 5′-ATATGCCGCACATCCCCTAT-3′, followed by restriction digestion with Hpy188III (New England Biolabs, Inc.).

    Real-time Polymerase Chain Reaction:

    Article Title: Friedreich Ataxia Patient Tissues Exhibit Increased 5-Hydroxymethylcytosine Modification and Decreased CTCF Binding at the FXN Locus
    Article Snippet: The MSREs used for CpGs 3, 6, 11 and 13 were Aci I (Fermentas), Hpy 188III (New England Biolabs), Aji I (Fermentas) and Eco 72I (Fermentas), respectively. .. The MSREs used for CpGs 3, 6, 11 and 13 were Aci I (Fermentas), Hpy 188III (New England Biolabs), Aji I (Fermentas) and Eco 72I (Fermentas), respectively.

    Article Title: Generation and Characterisation of Friedreich Ataxia YG8R Mouse Fibroblast and Neural Stem Cell Models
    Article Snippet: The MSREs used for CpGs 3 and 6 were AciI (Fermentas), and Hpy188III (New England Biolabs), respectively . .. The MSREs used for CpGs 3 and 6 were AciI (Fermentas), and Hpy188III (New England Biolabs), respectively .

    Allele-specific Oligonucleotide:

    Article Title: Selected Aspects of Molecular Diagnostics of Constitutional Alterations in BRCA1 and BRCA2 Genes Associated with Increased Risk of Breast Cancer in the Polish Population
    Article Snippet: The IVS2+1G > A and 430T > C mutations were identified by RFLP-PCR using Hpy 188III (New England Biolabs). .. The IVS2+1G > A and 430T > C mutations were identified by RFLP-PCR using Hpy 188III (New England Biolabs).

    other:

    Article Title: FSH dystrophy and a subtelomeric 4q haplotype: a new assay and associations with disease
    Article Snippet: To analyse the association of the 4qA161 haplotype with FSHD, we identified a SNP ( , SNP3) located 307 bp proximal to D4Z4 (GenBank , nt 4519) at overlapping recognition sites for Hpy188I and Hpy188III ( ).

    Article Title: FSH dystrophy and a subtelomeric 4q haplotype: a new assay and associations with disease
    Article Snippet: Of the 11 examined FSHD DNA samples, all had at least one 4qA161 allele, as seen by the undigested band in the Hpy188I digest and a digested band in the Hpy188III digest ( ).

    Polymerase Chain Reaction:

    Article Title: Association of toll-like receptors polymorphism and intrauterine transmission of cytomegalovirus
    Article Snippet: The PCR amplification method was as follows: 3 min at 94°C followed by 40 cycles each of 30 seconds of denaturation at 95°C, 30 seconds annealing (rs4696480 at 67°C, rs3804100 at 60°C and rs179008 at 65°C) and 20 seconds elongation at 72°C. .. PCR products were digested for 1 hour at 37°C with Hpy188III (New England Biolabs, USA) for rs4696480, with HpyCH4III (New England Biolabs, USA) for rs3804100 and with ApoI-Fast Digest (Thermo Scientific, USA) for rs179008. .. The DNA fragments were analyzed on 2% agarose gel and the SNP variation was determined by band patterns.

    Article Title: Application of Oocyte Cryopreservation Technology in TALEN-Mediated Mouse Genome Editing
    Article Snippet: For RFLP analysis, the PCR products were precipitated with ethanol. .. Each sample was then digested at 37°C overnight in 10 µ l of digestion solution containing 1×CutSmart buffer and 1.25 U of Hph I (New England Biolabs, Tokyo, Japan) or Hpy 188III (New England Biolabs) for the bL andClec4b1 genes, respectively.

    Article Title: FSH dystrophy and a subtelomeric 4q haplotype: a new assay and associations with disease
    Article Snippet: Differential digestion at SNP3 should distinguish the 4qA161 allele from the other reported haplotypes of 4q and 10q ( ) based upon sequencing of at least three alleles per haplotype by Lemmers et al . .. We amplified a sequence that contains SNP3 from somatic cell hybrid (SCH) and genomic DNAs and digested aliquots of the PCR products with Hpy188I or Hpy188III. .. SCH DNAs were completely sensitive to Hpy188I and resistant to Hpy188III or vice versa, as expected ( and data not shown).

    Article Title: Sema3d controls collective endothelial cell migration by distinct mechanisms via Nrp1 and PlxnD1
    Article Snippet: Nls-zCas9-nls mRNA was synthesized as previously described ( ). .. 2 nl of sema3d gRNA (12.5 ng/µl) and nls-zCas9-nls -mRNA (300 ng/µl) were injected into one-cell-stage zebrafish embryos. sema3d mutant genotypes were analyzed by PCR using sema3d geno SE, 5′-CTGCCGAAGCAAATCTCCTA-3′, and AS, 5′-ATATGCCGCACATCCCCTAT-3′, followed by restriction digestion with Hpy188III (New England Biolabs, Inc.). .. The fli1a:lifeactEGFP plasmid was generated via gateway cloning using Lifeact-EGFP ( ) and pTolfli1epDest ( ).

    Article Title: Detection and Quantification of Functional Genes of Cellulose- Degrading, Fermentative, and Sulfate-Reducing Bacteria and Methanogenic Archaea
    Article Snippet: The NEBcutter (version 2.0) tool of REBASE was used to identify suitable restriction enzymes and the respective cleavage sites. .. The Hpy188III, Mbol, MnlI, NlaIII, and MboII restriction enzymes (New England Biolabs, Ipswich, MA) were chosen to digest the PCR products from the primer sets targeting the cel5 , cel48 , hydA , dsrA , and mcrA genes, respectively. .. M13 PCR products of cloned gene inserts were digested with the corresponding restriction enzymes according to the manufacturer's recommendations, with incubation conditions of 37°C for 1 h ( mcrA ), 1.5 h ( cel5 and hydA ), or 3 h ( cel48 and dsrA ), followed by an inactivation step of 20 min at 65°C.

    Article Title: FSH dystrophy and a subtelomeric 4q haplotype: a new assay and associations with disease
    Article Snippet: Genomic DNA (100 ng) was amplified in a 25-µl volume using 0.2 µM primers 1-F (5′-CTGTGGTCATCTCTGCTCCA-3′) and 1-R (5′-TTTGCCTGTGAGTTCGAATG-3′) as follows: 95°C, 2 min; then 45 cycles of 95°C, 20 s; 60°C, 20 s; 72°C, 20 s; followed by 72°C for 5 min. For each set of PCRs, a no-template control was the last sample. .. Aliquots of the single 168-bp PCR product (10 µl) were digested with 20 U of Hpy188I or of Hpy188III (New England Biolabs) for 3 h at 37°C (40 µl reaction volume). .. For Hpy188III, first 10 U was added, followed by 5 U after 1 and again after 2 h. Digests were electrophoresed in a 2.5% or 3% agarose gel for 3 h, followed by ethidium bromide staining (1 µg/ml) for 30 min. For about half of the reactions, 0.1 µg of a pUC19 plasmid DNA was added as an internal control to an aliquot of the digest and incubated concurrently.

    Article Title: Human PTRF mutations cause secondary deficiency of caveolins resulting in muscular dystrophy with generalized lipodystrophy
    Article Snippet: To confirm the compound heterozygosity in P5, the PCR product was cloned and sequenced. .. In order to determine the frequency of the mutations in PTRF , we performed enzyme digestion of PCR products from 200 Japanese control subjects using Hpy 188III (New England Biolabs) for c.696_697insC and Taq I (New England Biolabs) for c.525delG. .. Mbo II (New England Biolabs) was used for enzyme digestion of PCR products to detect the c.1138G > A substitution in BSCL2 .

    Article Title: Friedreich Ataxia Patient Tissues Exhibit Increased 5-Hydroxymethylcytosine Modification and Decreased CTCF Binding at the FXN Locus
    Article Snippet: The MSREs used for CpGs 3, 6, 11 and 13 were Aci I (Fermentas), Hpy 188III (New England Biolabs), Aji I (Fermentas) and Eco 72I (Fermentas), respectively. .. A 50ng aliquot of digested DNA was then amplified by quantitative PCR using SYBR® Green (Applied Biosystems) and an ABI7900HT Fast Real-Time PCR System with the following primers: CpG3 F 5’-GAGACGTGGCTTTGTTTTCTG-3’ and R 5’-GTTTCCTCCTTTCAAGCCGTG-3’ ; CpG6 F 5’-GAAGATGCCAAGGAAGTGGTAG-3’ and R 5’-GAGCAACACAAATATGGCTTGG-3’ ; CpG11 and CpG13 F-met 5’-GAACCGTCTGGGCAAAGGCCAG-3’ and R-met 5’-ATCCCAAAGTTTCTTCAAACACAATG-3’ .

    Article Title: Generation and Characterisation of Friedreich Ataxia YG8R Mouse Fibroblast and Neural Stem Cell Models
    Article Snippet: The MSREs used for CpGs 3 and 6 were AciI (Fermentas), and Hpy188III (New England Biolabs), respectively . .. A 50ng aliquot of digested DNA was then amplified by quantitative PCR using SYBR® Green (Applied Biosystems) and an ABI 7900 Fast Real-Time PCR System (Applied Biosystems) with the following primers: CpG3 F 5′-GAGACGTGGCTTTGTTTTCTG-3′ and R 5′-GTTTCCTCCTTTCAAGCCGTG-3′ ; CpG6 F 5′-GAAGATGCCAAGGAAGTGGTAG-3′ and R 5′-GAGCAACACAAATATGGCTTGG-3′ .

    Article Title: Association of the Serotonin Receptor 3E Gene as a Functional Variant in the MicroRNA-510 Target Site with Diarrhea Predominant Irritable Bowel Syndrome in Chinese Women
    Article Snippet: SNP were identified by restriction enzyme digestion. .. Briefly, 3 μL of the PCR product of HTR3E was digested with 0.5 μL of the restriction enzyme Hpy188III (New England Biolabs, Ipswich, MA, USA), according to the manufacturer’s recommendations. .. Two types of genotypes were determined through electrophoresis of the enzyme-digested product of HTR3E 3′UTR, GG (298 bp), and GA (344 and 298 bp).

    Article Title: Polymorphisms in gyrA and gyrB Genes among Mycobacterium avium subsp. paratuberculosis Type I, II, and III Isolates
    Article Snippet: A PCR-REA was designed to detect the polymorphism in the gyrB gene at position 1626, the SNP that allowed types I and II to be distinguished from type III. .. The 897-bp PCR products were submitted to restriction analysis with the enzyme Hpy188-III (New England Biolabs). .. Digestion was performed with 10 μl of PCR products, 5 U of enzyme, 1× NE buffer 4, and 0.5 μl of 100 μg/ml bovine serum albumin.

    Article Title: Demethylation of promoter C region of estrogen receptor ? gene is correlated with its enhanced expression in estrogen-ablation resistant MCF-7 cells
    Article Snippet: PCR amplification of ERα promoter C region using bisulfite-converted DNA fragments was performed as described above except for the cycle numbers to be 50. .. For COBRA, 5 μl of purified DNA fragment was digested in a total volume of 20 μl using 5 units of Hpy 188III restriction enzyme (New England BioLabs, Ipswich, MA) that cleave CpG sites retained because of methylation at 37 °C for 4 h. The resultant DNA fragments together with undigested ones were electrophoresed onto 8% polyacrylamide gel.

    Injection:

    Article Title: Sema3d controls collective endothelial cell migration by distinct mechanisms via Nrp1 and PlxnD1
    Article Snippet: Nls-zCas9-nls mRNA was synthesized as previously described ( ). .. 2 nl of sema3d gRNA (12.5 ng/µl) and nls-zCas9-nls -mRNA (300 ng/µl) were injected into one-cell-stage zebrafish embryos. sema3d mutant genotypes were analyzed by PCR using sema3d geno SE, 5′-CTGCCGAAGCAAATCTCCTA-3′, and AS, 5′-ATATGCCGCACATCCCCTAT-3′, followed by restriction digestion with Hpy188III (New England Biolabs, Inc.). .. The fli1a:lifeactEGFP plasmid was generated via gateway cloning using Lifeact-EGFP ( ) and pTolfli1epDest ( ).

    Combined Bisulfite Restriction Analysis Assay:

    Article Title: Demethylation of promoter C region of estrogen receptor ? gene is correlated with its enhanced expression in estrogen-ablation resistant MCF-7 cells
    Article Snippet: Direct sequencing of the fragments was conducted using ERPROCBSF as a primer, BigDye Terminators v1.1 Cycle Sequencing Kit and ABI PRISM 310 Genetic Analyzer (Applied Biosystems, Foster City, CA). .. For COBRA, 5 μl of purified DNA fragment was digested in a total volume of 20 μl using 5 units of Hpy 188III restriction enzyme (New England BioLabs, Ipswich, MA) that cleave CpG sites retained because of methylation at 37 °C for 4 h. The resultant DNA fragments together with undigested ones were electrophoresed onto 8% polyacrylamide gel. .. After staining with ethidium bromide, the image was visualized under UV illumination.

    Fluorescence:

    Article Title: Demethylation of promoter C region of estrogen receptor ? gene is correlated with its enhanced expression in estrogen-ablation resistant MCF-7 cells
    Article Snippet: For COBRA, 5 μl of purified DNA fragment was digested in a total volume of 20 μl using 5 units of Hpy 188III restriction enzyme (New England BioLabs, Ipswich, MA) that cleave CpG sites retained because of methylation at 37 °C for 4 h. The resultant DNA fragments together with undigested ones were electrophoresed onto 8% polyacrylamide gel. .. After staining with ethidium bromide, the image was visualized under UV illumination.

    Methylation:

    Article Title: N6-methyladenine is an epigenetic marker of mammalian early life stress
    Article Snippet: Paragraph title: Enzyme-based Methylation Analysis ... Primers were designed such that some combination of HpaII (New England Biolabs, Ipswich, MA; catalog #R0171), HhaI (New England Biolabs; catalog #R0139), or Hpy188III (New England Biolabs; catalog #R0622) cut sites were inside or outside of the primer sequence.

    Article Title: Friedreich Ataxia Patient Tissues Exhibit Increased 5-Hydroxymethylcytosine Modification and Decreased CTCF Binding at the FXN Locus
    Article Snippet: 1µg of genomic DNA was digested with: (1) a methylation-sensitive restriction enzyme (MSRE), (2) a methylation-dependent restriction enzyme (MDRE), (3) both MSRE and MDRE (double digest, DD), and (4) neither MSRE or MDRE (mock control). .. The MSREs used for CpGs 3, 6, 11 and 13 were Aci I (Fermentas), Hpy 188III (New England Biolabs), Aji I (Fermentas) and Eco 72I (Fermentas), respectively.

    Article Title: Generation and Characterisation of Friedreich Ataxia YG8R Mouse Fibroblast and Neural Stem Cell Models
    Article Snippet: DNA methylation analysis was performed using the ‘MethylScreen’ method , which uses combined restriction digestion of DNA with methylation sensitive and methylation dependent restriction enzymes, MSRE and MDRE respectively. .. The MSREs used for CpGs 3 and 6 were AciI (Fermentas), and Hpy188III (New England Biolabs), respectively .

    Article Title: Demethylation of promoter C region of estrogen receptor ? gene is correlated with its enhanced expression in estrogen-ablation resistant MCF-7 cells
    Article Snippet: Direct sequencing of the fragments was conducted using ERPROCBSF as a primer, BigDye Terminators v1.1 Cycle Sequencing Kit and ABI PRISM 310 Genetic Analyzer (Applied Biosystems, Foster City, CA). .. For COBRA, 5 μl of purified DNA fragment was digested in a total volume of 20 μl using 5 units of Hpy 188III restriction enzyme (New England BioLabs, Ipswich, MA) that cleave CpG sites retained because of methylation at 37 °C for 4 h. The resultant DNA fragments together with undigested ones were electrophoresed onto 8% polyacrylamide gel. .. After staining with ethidium bromide, the image was visualized under UV illumination.

    Mutagenesis:

    Article Title: Application of Oocyte Cryopreservation Technology in TALEN-Mediated Mouse Genome Editing
    Article Snippet: Paragraph title: Analyses for mutant screening ... Each sample was then digested at 37°C overnight in 10 µ l of digestion solution containing 1×CutSmart buffer and 1.25 U of Hph I (New England Biolabs, Tokyo, Japan) or Hpy 188III (New England Biolabs) for the bL andClec4b1 genes, respectively.

    Article Title: Sema3d controls collective endothelial cell migration by distinct mechanisms via Nrp1 and PlxnD1
    Article Snippet: Nls-zCas9-nls mRNA was synthesized as previously described ( ). .. 2 nl of sema3d gRNA (12.5 ng/µl) and nls-zCas9-nls -mRNA (300 ng/µl) were injected into one-cell-stage zebrafish embryos. sema3d mutant genotypes were analyzed by PCR using sema3d geno SE, 5′-CTGCCGAAGCAAATCTCCTA-3′, and AS, 5′-ATATGCCGCACATCCCCTAT-3′, followed by restriction digestion with Hpy188III (New England Biolabs, Inc.). .. The fli1a:lifeactEGFP plasmid was generated via gateway cloning using Lifeact-EGFP ( ) and pTolfli1epDest ( ).

    Article Title: Human PTRF mutations cause secondary deficiency of caveolins resulting in muscular dystrophy with generalized lipodystrophy
    Article Snippet: Paragraph title: Mutation screening and haplotype analysis. ... In order to determine the frequency of the mutations in PTRF , we performed enzyme digestion of PCR products from 200 Japanese control subjects using Hpy 188III (New England Biolabs) for c.696_697insC and Taq I (New England Biolabs) for c.525delG.

    Article Title: POWERDRESS and Diversified Expression of the MIR172 Gene Family Bolster the Floral Stem Cell Network
    Article Snippet: The ag-10 mutation eliminates an HpyA V restriction site and was genotyped using primers AGp1 and ag10_genoR followed by HpyA V digestion (NEB, Cat# R0621). .. The fragment amplified from miR172d-1 fails to be cut by Hpy188 III (NEB, Cat# R0622).

    Isolation:

    Article Title: Human PTRF mutations cause secondary deficiency of caveolins resulting in muscular dystrophy with generalized lipodystrophy
    Article Snippet: Genomic DNA was isolated from peripheral lymphocytes or muscles using standard techniques. .. In order to determine the frequency of the mutations in PTRF , we performed enzyme digestion of PCR products from 200 Japanese control subjects using Hpy 188III (New England Biolabs) for c.696_697insC and Taq I (New England Biolabs) for c.525delG.

    Purification:

    Article Title: Demethylation of promoter C region of estrogen receptor ? gene is correlated with its enhanced expression in estrogen-ablation resistant MCF-7 cells
    Article Snippet: Direct sequencing of the fragments was conducted using ERPROCBSF as a primer, BigDye Terminators v1.1 Cycle Sequencing Kit and ABI PRISM 310 Genetic Analyzer (Applied Biosystems, Foster City, CA). .. For COBRA, 5 μl of purified DNA fragment was digested in a total volume of 20 μl using 5 units of Hpy 188III restriction enzyme (New England BioLabs, Ipswich, MA) that cleave CpG sites retained because of methylation at 37 °C for 4 h. The resultant DNA fragments together with undigested ones were electrophoresed onto 8% polyacrylamide gel. .. After staining with ethidium bromide, the image was visualized under UV illumination.

    Sequencing:

    Article Title: Application of Oocyte Cryopreservation Technology in TALEN-Mediated Mouse Genome Editing
    Article Snippet: Each sample was then digested at 37°C overnight in 10 µ l of digestion solution containing 1×CutSmart buffer and 1.25 U of Hph I (New England Biolabs, Tokyo, Japan) or Hpy 188III (New England Biolabs) for the bL andClec4b1 genes, respectively. .. The digested products were subjected to agarose gel electrophoresis and ethidium bromide staining.

    Article Title: N6-methyladenine is an epigenetic marker of mammalian early life stress
    Article Snippet: 5 mC DNA methylation was assessed using an adapted version of the methylation sensitive restriction enzyme (MSRE) assay , as previously described , . .. Primers were designed such that some combination of HpaII (New England Biolabs, Ipswich, MA; catalog #R0171), HhaI (New England Biolabs; catalog #R0139), or Hpy188III (New England Biolabs; catalog #R0622) cut sites were inside or outside of the primer sequence. .. 6 mA DNA methylation was determined by methylation dependent restriction enzyme (MDRE) analysis.

    Article Title: FSH dystrophy and a subtelomeric 4q haplotype: a new assay and associations with disease
    Article Snippet: Differential digestion at SNP3 should distinguish the 4qA161 allele from the other reported haplotypes of 4q and 10q ( ) based upon sequencing of at least three alleles per haplotype by Lemmers et al . .. We amplified a sequence that contains SNP3 from somatic cell hybrid (SCH) and genomic DNAs and digested aliquots of the PCR products with Hpy188I or Hpy188III. .. SCH DNAs were completely sensitive to Hpy188I and resistant to Hpy188III or vice versa, as expected ( and data not shown).

    Article Title: Human PTRF mutations cause secondary deficiency of caveolins resulting in muscular dystrophy with generalized lipodystrophy
    Article Snippet: In order to determine the frequency of the mutations in PTRF , we performed enzyme digestion of PCR products from 200 Japanese control subjects using Hpy 188III (New England Biolabs) for c.696_697insC and Taq I (New England Biolabs) for c.525delG. .. In order to determine the frequency of the mutations in PTRF , we performed enzyme digestion of PCR products from 200 Japanese control subjects using Hpy 188III (New England Biolabs) for c.696_697insC and Taq I (New England Biolabs) for c.525delG.

    Article Title: Association of the Serotonin Receptor 3E Gene as a Functional Variant in the MicroRNA-510 Target Site with Diarrhea Predominant Irritable Bowel Syndrome in Chinese Women
    Article Snippet: Briefly, 3 μL of the PCR product of HTR3E was digested with 0.5 μL of the restriction enzyme Hpy188III (New England Biolabs, Ipswich, MA, USA), according to the manufacturer’s recommendations. .. Two types of genotypes were determined through electrophoresis of the enzyme-digested product of HTR3E 3′UTR, GG (298 bp), and GA (344 and 298 bp).

    Article Title: Demethylation of promoter C region of estrogen receptor ? gene is correlated with its enhanced expression in estrogen-ablation resistant MCF-7 cells
    Article Snippet: Direct sequencing of the fragments was conducted using ERPROCBSF as a primer, BigDye Terminators v1.1 Cycle Sequencing Kit and ABI PRISM 310 Genetic Analyzer (Applied Biosystems, Foster City, CA). .. For COBRA, 5 μl of purified DNA fragment was digested in a total volume of 20 μl using 5 units of Hpy 188III restriction enzyme (New England BioLabs, Ipswich, MA) that cleave CpG sites retained because of methylation at 37 °C for 4 h. The resultant DNA fragments together with undigested ones were electrophoresed onto 8% polyacrylamide gel.

    Terminal Restriction Fragment Length Polymorphism:

    Article Title: Abundance and diversity of mucosa-associated hydrogenotrophic microbes in the healthy human colon
    Article Snippet: Paragraph title: Terminal restriction fragment length polymorphism analysis ... For mcrA , 10 μl aliquots (6.0 ng of DNA per μl) of nested PCR amplicons were digested with Dde I and Hpy 188III endonucleases (NE Biolabs).

    CRISPR:

    Article Title: Sema3d controls collective endothelial cell migration by distinct mechanisms via Nrp1 and PlxnD1
    Article Snippet: Paragraph title: Generation of sema3d mutants by CRISPR/Cas9-mediated mutagenesis ... 2 nl of sema3d gRNA (12.5 ng/µl) and nls-zCas9-nls -mRNA (300 ng/µl) were injected into one-cell-stage zebrafish embryos. sema3d mutant genotypes were analyzed by PCR using sema3d geno SE, 5′-CTGCCGAAGCAAATCTCCTA-3′, and AS, 5′-ATATGCCGCACATCCCCTAT-3′, followed by restriction digestion with Hpy188III (New England Biolabs, Inc.).

    Nested PCR:

    Article Title: Abundance and diversity of mucosa-associated hydrogenotrophic microbes in the healthy human colon
    Article Snippet: For the analysis of dsrA , 10 μl aliquots (6.0 ng of DNA per μl) of nested PCR amplicons were digested with Sau 96I and Bst UI endonucleases (NE Biolabs). .. For mcrA , 10 μl aliquots (6.0 ng of DNA per μl) of nested PCR amplicons were digested with Dde I and Hpy 188III endonucleases (NE Biolabs). .. TRF profiles were aligned on the basis of TRF lengths and individual peak areas using the moving average algorithm included in the T-Align software , resulting in the generation of data sets of aligned TRFs.

    Plasmid Preparation:

    Article Title: Sema3d controls collective endothelial cell migration by distinct mechanisms via Nrp1 and PlxnD1
    Article Snippet: Annealed CRISPR target site primers (CRIsema3d SE, 5′-TAGGAGACAGGTCAGGACGGGA-3′, and AS, 5′-AAACTCCCGTCCTGACCTGTCT-3′) were cloned into the guide RNA (gRNA) expression vector pDR274 as previously described ( ). .. 2 nl of sema3d gRNA (12.5 ng/µl) and nls-zCas9-nls -mRNA (300 ng/µl) were injected into one-cell-stage zebrafish embryos. sema3d mutant genotypes were analyzed by PCR using sema3d geno SE, 5′-CTGCCGAAGCAAATCTCCTA-3′, and AS, 5′-ATATGCCGCACATCCCCTAT-3′, followed by restriction digestion with Hpy188III (New England Biolabs, Inc.).

    Article Title: FSH dystrophy and a subtelomeric 4q haplotype: a new assay and associations with disease
    Article Snippet: Aliquots of the single 168-bp PCR product (10 µl) were digested with 20 U of Hpy188I or of Hpy188III (New England Biolabs) for 3 h at 37°C (40 µl reaction volume). .. For Hpy188III, first 10 U was added, followed by 5 U after 1 and again after 2 h. Digests were electrophoresed in a 2.5% or 3% agarose gel for 3 h, followed by ethidium bromide staining (1 µg/ml) for 30 min. For about half of the reactions, 0.1 µg of a pUC19 plasmid DNA was added as an internal control to an aliquot of the digest and incubated concurrently.

    Article Title: FSH dystrophy and a subtelomeric 4q haplotype: a new assay and associations with disease
    Article Snippet: Aliquots of the single 168-bp PCR product (10 µl) were digested with 20 U of Hpy188I or of Hpy188III (New England Biolabs) for 3 h at 37°C (40 µl reaction volume). .. For Hpy188III, first 10 U was added, followed by 5 U after 1 and again after 2 h. Digests were electrophoresed in a 2.5% or 3% agarose gel for 3 h, followed by ethidium bromide staining (1 µg/ml) for 30 min. For about half of the reactions, 0.1 µg of a pUC19 plasmid DNA was added as an internal control to an aliquot of the digest and incubated concurrently. .. Upon electrophoresis, the internal controls showed complete digestion relative to plasmid DNA in the absence of PCR product.

    Software:

    Article Title: Friedreich Ataxia Patient Tissues Exhibit Increased 5-Hydroxymethylcytosine Modification and Decreased CTCF Binding at the FXN Locus
    Article Snippet: The MSREs used for CpGs 3, 6, 11 and 13 were Aci I (Fermentas), Hpy 188III (New England Biolabs), Aji I (Fermentas) and Eco 72I (Fermentas), respectively. .. A 50ng aliquot of digested DNA was then amplified by quantitative PCR using SYBR® Green (Applied Biosystems) and an ABI7900HT Fast Real-Time PCR System with the following primers: CpG3 F 5’-GAGACGTGGCTTTGTTTTCTG-3’ and R 5’-GTTTCCTCCTTTCAAGCCGTG-3’ ; CpG6 F 5’-GAAGATGCCAAGGAAGTGGTAG-3’ and R 5’-GAGCAACACAAATATGGCTTGG-3’ ; CpG11 and CpG13 F-met 5’-GAACCGTCTGGGCAAAGGCCAG-3’ and R-met 5’-ATCCCAAAGTTTCTTCAAACACAATG-3’ .

    Article Title: Generation and Characterisation of Friedreich Ataxia YG8R Mouse Fibroblast and Neural Stem Cell Models
    Article Snippet: The MSREs used for CpGs 3 and 6 were AciI (Fermentas), and Hpy188III (New England Biolabs), respectively . .. A 50ng aliquot of digested DNA was then amplified by quantitative PCR using SYBR® Green (Applied Biosystems) and an ABI 7900 Fast Real-Time PCR System (Applied Biosystems) with the following primers: CpG3 F 5′-GAGACGTGGCTTTGTTTTCTG-3′ and R 5′-GTTTCCTCCTTTCAAGCCGTG-3′ ; CpG6 F 5′-GAAGATGCCAAGGAAGTGGTAG-3′ and R 5′-GAGCAACACAAATATGGCTTGG-3′ .

    Electrophoresis:

    Article Title: FSH dystrophy and a subtelomeric 4q haplotype: a new assay and associations with disease
    Article Snippet: Aliquots of the single 168-bp PCR product (10 µl) were digested with 20 U of Hpy188I or of Hpy188III (New England Biolabs) for 3 h at 37°C (40 µl reaction volume). .. For Hpy188III, first 10 U was added, followed by 5 U after 1 and again after 2 h. Digests were electrophoresed in a 2.5% or 3% agarose gel for 3 h, followed by ethidium bromide staining (1 µg/ml) for 30 min. For about half of the reactions, 0.1 µg of a pUC19 plasmid DNA was added as an internal control to an aliquot of the digest and incubated concurrently.

    Article Title: Polymorphisms in gyrA and gyrB Genes among Mycobacterium avium subsp. paratuberculosis Type I, II, and III Isolates
    Article Snippet: The 897-bp PCR products were submitted to restriction analysis with the enzyme Hpy188-III (New England Biolabs). .. Digestion was performed with 10 μl of PCR products, 5 U of enzyme, 1× NE buffer 4, and 0.5 μl of 100 μg/ml bovine serum albumin.

    Functional Assay:

    Article Title: Detection and Quantification of Functional Genes of Cellulose- Degrading, Fermentative, and Sulfate-Reducing Bacteria and Methanogenic Archaea
    Article Snippet: The Restriction Enzyme Database (REBASE) ( ) was used to select restriction endonucleases that could digest each functional gene PCR product. .. The Hpy188III, Mbol, MnlI, NlaIII, and MboII restriction enzymes (New England Biolabs, Ipswich, MA) were chosen to digest the PCR products from the primer sets targeting the cel5 , cel48 , hydA , dsrA , and mcrA genes, respectively.

    Selection:

    Article Title: Detection and Quantification of Functional Genes of Cellulose- Degrading, Fermentative, and Sulfate-Reducing Bacteria and Methanogenic Archaea
    Article Snippet: Paragraph title: Selection of restriction enzymes. ... The Hpy188III, Mbol, MnlI, NlaIII, and MboII restriction enzymes (New England Biolabs, Ipswich, MA) were chosen to digest the PCR products from the primer sets targeting the cel5 , cel48 , hydA , dsrA , and mcrA genes, respectively.

    Agarose Gel Electrophoresis:

    Article Title: Application of Oocyte Cryopreservation Technology in TALEN-Mediated Mouse Genome Editing
    Article Snippet: Each PCR product was subjected to agarose gel electrophoresis and ethidium bromide staining for the HMA. .. Each sample was then digested at 37°C overnight in 10 µ l of digestion solution containing 1×CutSmart buffer and 1.25 U of Hph I (New England Biolabs, Tokyo, Japan) or Hpy 188III (New England Biolabs) for the bL andClec4b1 genes, respectively.

    Article Title: FSH dystrophy and a subtelomeric 4q haplotype: a new assay and associations with disease
    Article Snippet: Aliquots of the single 168-bp PCR product (10 µl) were digested with 20 U of Hpy188I or of Hpy188III (New England Biolabs) for 3 h at 37°C (40 µl reaction volume). .. For Hpy188III, first 10 U was added, followed by 5 U after 1 and again after 2 h. Digests were electrophoresed in a 2.5% or 3% agarose gel for 3 h, followed by ethidium bromide staining (1 µg/ml) for 30 min. For about half of the reactions, 0.1 µg of a pUC19 plasmid DNA was added as an internal control to an aliquot of the digest and incubated concurrently. .. Upon electrophoresis, the internal controls showed complete digestion relative to plasmid DNA in the absence of PCR product.

    Article Title: Association of the Serotonin Receptor 3E Gene as a Functional Variant in the MicroRNA-510 Target Site with Diarrhea Predominant Irritable Bowel Syndrome in Chinese Women
    Article Snippet: A 3 μL aliquot of the PCR product was run on 1.5% agarose gel to visualize a 397-bp fragment. .. Briefly, 3 μL of the PCR product of HTR3E was digested with 0.5 μL of the restriction enzyme Hpy188III (New England Biolabs, Ipswich, MA, USA), according to the manufacturer’s recommendations.

    Article Title: Polymorphisms in gyrA and gyrB Genes among Mycobacterium avium subsp. paratuberculosis Type I, II, and III Isolates
    Article Snippet: The 897-bp PCR products were submitted to restriction analysis with the enzyme Hpy188-III (New England Biolabs). .. Digestion was performed with 10 μl of PCR products, 5 U of enzyme, 1× NE buffer 4, and 0.5 μl of 100 μg/ml bovine serum albumin.

    DNA Methylation Assay:

    Article Title: N6-methyladenine is an epigenetic marker of mammalian early life stress
    Article Snippet: 5 mC DNA methylation was assessed using an adapted version of the methylation sensitive restriction enzyme (MSRE) assay , as previously described , . .. Primers were designed such that some combination of HpaII (New England Biolabs, Ipswich, MA; catalog #R0171), HhaI (New England Biolabs; catalog #R0139), or Hpy188III (New England Biolabs; catalog #R0622) cut sites were inside or outside of the primer sequence.

    Article Title: Generation and Characterisation of Friedreich Ataxia YG8R Mouse Fibroblast and Neural Stem Cell Models
    Article Snippet: DNA methylation analysis was performed using the ‘MethylScreen’ method , which uses combined restriction digestion of DNA with methylation sensitive and methylation dependent restriction enzymes, MSRE and MDRE respectively. .. The MSREs used for CpGs 3 and 6 were AciI (Fermentas), and Hpy188III (New England Biolabs), respectively .

    CTG Assay:

    Article Title: Association of toll-like receptors polymorphism and intrauterine transmission of cytomegalovirus
    Article Snippet: The primers: F- GTT CTG GAG TCT GGG AAG TC and R-AAT GTT ATC ACC AAG GGA GCA G were used for rs4696480 and gave a product size of 171 bp. .. PCR products were digested for 1 hour at 37°C with Hpy188III (New England Biolabs, USA) for rs4696480, with HpyCH4III (New England Biolabs, USA) for rs3804100 and with ApoI-Fast Digest (Thermo Scientific, USA) for rs179008.

    Marker:

    Article Title: Polymorphisms in gyrA and gyrB Genes among Mycobacterium avium subsp. paratuberculosis Type I, II, and III Isolates
    Article Snippet: The 897-bp PCR products were submitted to restriction analysis with the enzyme Hpy188-III (New England Biolabs). .. Digestion was performed with 10 μl of PCR products, 5 U of enzyme, 1× NE buffer 4, and 0.5 μl of 100 μg/ml bovine serum albumin.

    Staining:

    Article Title: Application of Oocyte Cryopreservation Technology in TALEN-Mediated Mouse Genome Editing
    Article Snippet: Each PCR product was subjected to agarose gel electrophoresis and ethidium bromide staining for the HMA. .. Each sample was then digested at 37°C overnight in 10 µ l of digestion solution containing 1×CutSmart buffer and 1.25 U of Hph I (New England Biolabs, Tokyo, Japan) or Hpy 188III (New England Biolabs) for the bL andClec4b1 genes, respectively.

    Article Title: FSH dystrophy and a subtelomeric 4q haplotype: a new assay and associations with disease
    Article Snippet: Aliquots of the single 168-bp PCR product (10 µl) were digested with 20 U of Hpy188I or of Hpy188III (New England Biolabs) for 3 h at 37°C (40 µl reaction volume). .. For Hpy188III, first 10 U was added, followed by 5 U after 1 and again after 2 h. Digests were electrophoresed in a 2.5% or 3% agarose gel for 3 h, followed by ethidium bromide staining (1 µg/ml) for 30 min. For about half of the reactions, 0.1 µg of a pUC19 plasmid DNA was added as an internal control to an aliquot of the digest and incubated concurrently. .. Upon electrophoresis, the internal controls showed complete digestion relative to plasmid DNA in the absence of PCR product.

    Article Title: Polymorphisms in gyrA and gyrB Genes among Mycobacterium avium subsp. paratuberculosis Type I, II, and III Isolates
    Article Snippet: The 897-bp PCR products were submitted to restriction analysis with the enzyme Hpy188-III (New England Biolabs). .. Digestion was performed with 10 μl of PCR products, 5 U of enzyme, 1× NE buffer 4, and 0.5 μl of 100 μg/ml bovine serum albumin.

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    New England Biolabs hpy188 iii
    Hpy188 Iii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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