ag 10 mutation  (New England Biolabs)


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    Name:
    HpyAV
    Description:
    HpyAV 500 units
    Catalog Number:
    r0621l
    Price:
    290
    Size:
    500 units
    Category:
    Restriction Enzymes
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    Structured Review

    New England Biolabs ag 10 mutation
    HpyAV
    HpyAV 500 units
    https://www.bioz.com/result/ag 10 mutation/product/New England Biolabs
    Average 85 stars, based on 27 article reviews
    Price from $9.99 to $1999.99
    ag 10 mutation - by Bioz Stars, 2020-07
    85/100 stars

    Images

    1) Product Images from "POWERDRESS and Diversified Expression of the MIR172 Gene Family Bolster the Floral Stem Cell Network"

    Article Title: POWERDRESS and Diversified Expression of the MIR172 Gene Family Bolster the Floral Stem Cell Network

    Journal: PLoS Genetics

    doi: 10.1371/journal.pgen.1003218

    Phenotypes of ag-10 pwr-1 in combination with loss-of-function mutations in other floral determinacy regulators. (A) clv3-1 flower with supernumerary organs in all four whorls. (B) clv3-1 ag-10 pwr-1 triple mutant flower. Compared to clv3-1 , flowers of the triple mutant are small, have more carpels, and have shortened and bulged gynoecia often containing internal flowers. (C) sup-1 flower with aberrant carpels and more stamens than wild-type. (D) sup-1 ag-10 double mutant flower. These flowers are more strongly indeterminate compared to sup-1 : more stamens are produced, and a mass of undifferentiated cells is visible at the center of the flower (magnified and indicated with an arrow in the inset). (E) sup-1 ag-10 pwr-1 triple mutant flower. (F) ag-1 (left) and ag-1 pwr-1 (right) flowers. pwr-1 does not enhance the indeterminacy defects of ag-1 flowers. (G) wus-1 flower exhibiting premature termination of floral development. (H) wus-1 ag-10 pwr-1 flower. Along with wus-1 pwr-1 (not shown), flowers of the triple mutant resemble wus-1 single mutant flowers. (I) ag-10 pwr-1 (left) and ap2-2 ag-10 pwr-1 (right) gynoecia. The ap2-2 mutation reduced the indeterminacy defects of ag-10 pwr-1 : the triple mutant gynoecia were less bulged compared to ag-10 pwr-1 , and a smaller percentage contained internal floral organs. (J) Longitudinal section of a stage 7 ag-10 pWUS:GUS flower showing the absence of GUS staining in the floral meristem. (K) Longitudinal section of a stage 7 or stage 8 ag-10 pwr-1 pWUS:GUS flower showing GUS staining in the floral meristem. (L) Longitudinal section of a stage 11 ag-10 pwr-1 pWUS:GUS flower. (M) crc-1 flower and (N) crc-1 pwr-1 flower with floral organs partially removed to reveal the gynoecia. While crc-1 single mutant flowers have unfused carpels and rarely exhibit indeterminacy defects, approximately half of all crc-1 pwr-1 gynoecia dissected contained internal floral organs. Scale bars = 10 µm in (J) to (L), 5 mm in (I), and 1 mm in all other panels.
    Figure Legend Snippet: Phenotypes of ag-10 pwr-1 in combination with loss-of-function mutations in other floral determinacy regulators. (A) clv3-1 flower with supernumerary organs in all four whorls. (B) clv3-1 ag-10 pwr-1 triple mutant flower. Compared to clv3-1 , flowers of the triple mutant are small, have more carpels, and have shortened and bulged gynoecia often containing internal flowers. (C) sup-1 flower with aberrant carpels and more stamens than wild-type. (D) sup-1 ag-10 double mutant flower. These flowers are more strongly indeterminate compared to sup-1 : more stamens are produced, and a mass of undifferentiated cells is visible at the center of the flower (magnified and indicated with an arrow in the inset). (E) sup-1 ag-10 pwr-1 triple mutant flower. (F) ag-1 (left) and ag-1 pwr-1 (right) flowers. pwr-1 does not enhance the indeterminacy defects of ag-1 flowers. (G) wus-1 flower exhibiting premature termination of floral development. (H) wus-1 ag-10 pwr-1 flower. Along with wus-1 pwr-1 (not shown), flowers of the triple mutant resemble wus-1 single mutant flowers. (I) ag-10 pwr-1 (left) and ap2-2 ag-10 pwr-1 (right) gynoecia. The ap2-2 mutation reduced the indeterminacy defects of ag-10 pwr-1 : the triple mutant gynoecia were less bulged compared to ag-10 pwr-1 , and a smaller percentage contained internal floral organs. (J) Longitudinal section of a stage 7 ag-10 pWUS:GUS flower showing the absence of GUS staining in the floral meristem. (K) Longitudinal section of a stage 7 or stage 8 ag-10 pwr-1 pWUS:GUS flower showing GUS staining in the floral meristem. (L) Longitudinal section of a stage 11 ag-10 pwr-1 pWUS:GUS flower. (M) crc-1 flower and (N) crc-1 pwr-1 flower with floral organs partially removed to reveal the gynoecia. While crc-1 single mutant flowers have unfused carpels and rarely exhibit indeterminacy defects, approximately half of all crc-1 pwr-1 gynoecia dissected contained internal floral organs. Scale bars = 10 µm in (J) to (L), 5 mm in (I), and 1 mm in all other panels.

    Techniques Used: Mutagenesis, Produced, Staining

    Gene diagram of PWR and phenotypes of ag-10 , ag-10 pwr-1 , ag-10 Col , and ag-10 Col pwr-2 . (A) Schematic diagram indicating the position of the G-to-A mutation in pwr-1 that produces a premature stop codon and the approximate location of the T-DNA insertion in pwr-2 . The regions encoding the putative SANT domains are also indicated. (B) Wild-type L er flower. (C) ag-10 flower with slightly bulged carpels. (D) ag-10 pwr-1 flower with narrow, slightly folded petals and a shortened and bulged gynoecium. (E) Wild-type Col flower. (F) ag-10 Col flower. Col and ag-10 Col flowers are indistinguishable. (G) ag-10 Col pwr-2 flower with slightly aberrant carpels. (H) From left to right, siliques of L er , ag-10 , and ag-10 pwr-1 . ag-10 pwr-1 has visible gynophores bearing shortened and bulged gynoecia containing internal flowers. (I) From left to right, siliques of Col, ag-10 Col , and ag-10 Col pwr-2 . Although most ag-10 Col pwr-2 siliques have very subtle carpel defects, some are visibly bulged and contain additional floral organs inside. (J) Rescue analysis for ag-10 pwr-1 . From left to right, ag-10 , ag-10 pwr-1 , and pPWR:PWR-GFP in the ag-10 pwr-1 background. Scale bars = 500 bp in (A), 5 mm in (H) to (J), and 1 mm in all other panels.
    Figure Legend Snippet: Gene diagram of PWR and phenotypes of ag-10 , ag-10 pwr-1 , ag-10 Col , and ag-10 Col pwr-2 . (A) Schematic diagram indicating the position of the G-to-A mutation in pwr-1 that produces a premature stop codon and the approximate location of the T-DNA insertion in pwr-2 . The regions encoding the putative SANT domains are also indicated. (B) Wild-type L er flower. (C) ag-10 flower with slightly bulged carpels. (D) ag-10 pwr-1 flower with narrow, slightly folded petals and a shortened and bulged gynoecium. (E) Wild-type Col flower. (F) ag-10 Col flower. Col and ag-10 Col flowers are indistinguishable. (G) ag-10 Col pwr-2 flower with slightly aberrant carpels. (H) From left to right, siliques of L er , ag-10 , and ag-10 pwr-1 . ag-10 pwr-1 has visible gynophores bearing shortened and bulged gynoecia containing internal flowers. (I) From left to right, siliques of Col, ag-10 Col , and ag-10 Col pwr-2 . Although most ag-10 Col pwr-2 siliques have very subtle carpel defects, some are visibly bulged and contain additional floral organs inside. (J) Rescue analysis for ag-10 pwr-1 . From left to right, ag-10 , ag-10 pwr-1 , and pPWR:PWR-GFP in the ag-10 pwr-1 background. Scale bars = 500 bp in (A), 5 mm in (H) to (J), and 1 mm in all other panels.

    Techniques Used: Mutagenesis

    Phenotype of the mir172d-1 single mutant and of mir172d-1 in combination with ag-10 and ap2-2 . (A) ag-10 mir172d-1 flower. (B) L er (left) and mir172d-1 (right) siliques. The gynoecia of mir172d-1 occasionally have three fused carpels instead of two. (C) ap2-2 ag-10 mir172d-1 flower. (D) Mature miR172 sequences and the site of the G-to-A mutation in mir172d-1 (indicated by *).
    Figure Legend Snippet: Phenotype of the mir172d-1 single mutant and of mir172d-1 in combination with ag-10 and ap2-2 . (A) ag-10 mir172d-1 flower. (B) L er (left) and mir172d-1 (right) siliques. The gynoecia of mir172d-1 occasionally have three fused carpels instead of two. (C) ap2-2 ag-10 mir172d-1 flower. (D) Mature miR172 sequences and the site of the G-to-A mutation in mir172d-1 (indicated by *).

    Techniques Used: Mutagenesis

    Related Articles

    Mutagenesis:

    Article Title: POWERDRESS and Diversified Expression of the MIR172 Gene Family Bolster the Floral Stem Cell Network
    Article Snippet: .. The ag-10 mutation eliminates an HpyA V restriction site and was genotyped using primers AGp1 and ag10_genoR followed by HpyA V digestion (NEB, Cat# R0621). ..

    Polymerase Chain Reaction:

    Article Title: Genetic Control of Resistance to Trypanosoma brucei brucei Infection in Mice
    Article Snippet: .. Polymorphism of SNPs was tested by restriction analysis after PCR reaction using following restriction enzymes (New England BioLabs, Ipswich, MA): HpyAV for rs48212577 (14,13 µl of PCR product, 2 U (1 µl) of HpyAV, 1.7 µl of 10x NEB buffer 4 [200 mM Tris-acetate, 500 mM Potassium Acetate, 100 mM Magnesium Acetate, 10 mM Dithiothreitol, pH 7.9], 0.17 µl of 10 mg/ml BSA (bovine serum albumin), 37°C, o/n); HinfI for rs4229232 (14.8 µl of PCR product, 5 U (0,5 µl) of HinfI, 1.7 µl of 10x NEB buffer 4, 37°C, o/n); BsmFI for rs50154157 (14,13 µl of PCR product, 2 U (1 µl) of BsmFI, 1.7 µl of 10x NEB buffer 4, 0.17 µl of 10 mg/ml BSA, 65°C, o/n), and Tsp509I for rs50776991 (14,8 µl of PCR product, 2 U (0,5 µl) of Tsp509I, 1.7 µl of 10x NEB buffer 1 [100 mM Bis-Tris-propane-HCl, 100 mM MgCl2, 10 mM Dithiothreitol, pH 7.0], 65°C, o/n). .. The products were electrophoresed in 3% agarose gel containing 80% of MetaPhor® Agarose (Cambrex Bio Science Rockland, Inc., Rockland, ME) and 20% of UltraPure™ Agarose (Invitrogen, Carlsbad, CA) for 15 min to 2 h at 150 V.

    Binding Assay:

    Article Title: Fold or hold: experimental evolution in vitro
    Article Snippet: .. Extension then produces a new 3′ end … cCtt cGGTCTC*C* CACTGCTT-3′, capable of binding a primer supplied later in the protocol, and containing recognition sites for the two restriction enzymes BsaI and HpyAV (New England Biolabs, Ipswich, MA, USA) that cleave at the sites indicated by the asterisks. ..

    Amplification:

    Article Title: Molecular Diagnostics of Banana Fusarium Wilt Targeting Secreted-in-Xylem Genes
    Article Snippet: .. Restriction digestions for the enzymes HpyAV and EagI (New England Biolabs) were performed using 4 μL of the amplification product and 0.4 units (U) of enzyme in a total volume of 10 μL. ..

    other:

    Article Title: Molecular Diagnostics of Banana Fusarium Wilt Targeting Secreted-in-Xylem Genes
    Article Snippet: Therefore, following the PCRs targeting SIX1 and SIX13 , a restriction digestion step with the enzymes HpyAV and EagI was required to detect the specific homologs present in TR4 and R4 VCG 0122, respectively.

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    New England Biolabs enzymes hpyav
    Enzymes Hpyav, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/enzymes hpyav/product/New England Biolabs
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