baei  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Name:
    BaeI
    Description:
    BaeI 1 250 units
    Catalog Number:
    r0613l
    Price:
    269
    Size:
    1 250 units
    Category:
    Restriction Enzymes
    Buy from Supplier


    Structured Review

    New England Biolabs baei
    BaeI
    BaeI 1 250 units
    https://www.bioz.com/result/baei/product/New England Biolabs
    Average 93 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    baei - by Bioz Stars, 2020-09
    93/100 stars

    Images

    1) Product Images from "Terminal protein-primed amplification of heterologous DNA with a minimal replication system based on phage ?29"

    Article Title: Terminal protein-primed amplification of heterologous DNA with a minimal replication system based on phage ?29

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    doi: 10.1073/pnas.1114397108

    A pETORPHI derivative linearized with BaeI is efficient in amplification. ( A ) Scheme of BaeI recognition (underlined) and cutting sites (arrows) and position of Φ29 origins (capital letters) in pEYFPORBae. ( B ) Plasmid pEYFPORBae was prepared as
    Figure Legend Snippet: A pETORPHI derivative linearized with BaeI is efficient in amplification. ( A ) Scheme of BaeI recognition (underlined) and cutting sites (arrows) and position of Φ29 origins (capital letters) in pEYFPORBae. ( B ) Plasmid pEYFPORBae was prepared as

    Techniques Used: Amplification, Plasmid Preparation

    2) Product Images from "SLTAB2 is the paramutated SULFUREA locus in tomato"

    Article Title: SLTAB2 is the paramutated SULFUREA locus in tomato

    Journal: Journal of Experimental Botany

    doi: 10.1093/jxb/erw096

    SLTAB2 paramutation in the cross between a heterozygous sulf /+ and S. pimpinellifolium . (A) Phenotypes of a paramutated (left) and wild-type leaf (right) in the F1. (B) SLTAB2 expression in the F1. Paramutated plants showed reduced expression in the green sectors compared with wt plants ( P =7.8e-4, two-tailed t test), and even further reduction in the chlorotic sectors ( P =6.6e-3, paired t test between green and yellow samples). Data are plotted on a log 2 scale, the mean is represented by a horizontal bar. Paired data points for paramutated plants (green and yellow sectors) are joined by grey lines. (C) Only the S. pimpinellifolium SLTAB2 allele is expressed in paramutated F1 plants. The S. lycopersicum allele is sensitive to digestion by the BaeI restriction enzyme, resulting in a smear. Two SNPs in the S. pimpinellifolium SLTAB2 allele make it resistant to BaeI treatment. F1s expressing the S. lycopersicum allele show a smear, whereas F1s in which the S. lycopersicum allele is silent do not.
    Figure Legend Snippet: SLTAB2 paramutation in the cross between a heterozygous sulf /+ and S. pimpinellifolium . (A) Phenotypes of a paramutated (left) and wild-type leaf (right) in the F1. (B) SLTAB2 expression in the F1. Paramutated plants showed reduced expression in the green sectors compared with wt plants ( P =7.8e-4, two-tailed t test), and even further reduction in the chlorotic sectors ( P =6.6e-3, paired t test between green and yellow samples). Data are plotted on a log 2 scale, the mean is represented by a horizontal bar. Paired data points for paramutated plants (green and yellow sectors) are joined by grey lines. (C) Only the S. pimpinellifolium SLTAB2 allele is expressed in paramutated F1 plants. The S. lycopersicum allele is sensitive to digestion by the BaeI restriction enzyme, resulting in a smear. Two SNPs in the S. pimpinellifolium SLTAB2 allele make it resistant to BaeI treatment. F1s expressing the S. lycopersicum allele show a smear, whereas F1s in which the S. lycopersicum allele is silent do not.

    Techniques Used: Expressing, Two Tailed Test

    3) Product Images from "A novel approach to the generation of seamless constructs for plant transformation"

    Article Title: A novel approach to the generation of seamless constructs for plant transformation

    Journal: Plant Methods

    doi: 10.1186/1746-4811-10-10

    Overview of SRL used with In-Fusion™ cloning. Red letters indicate recognition sites and black triangles cleaving sites of LguI and BaeI . Red arrows indicate where In-Fusion™ enzyme exonuclease activity will occur. A . Spacer-removal linearization (SRL) of pAUrumII (to pAUrumII LIN ) with type IIS RE LguI . The spacer holds the LacZα coding sequence with its promoter. B . SRL of pAUrumIII (to pAUrumIII LIN ) with type IIB RE BaeI . C . Extended primers used in PCR amplification of gfp . The In-Fusion™-ready product, gfp IFR , has 15 nt of homology with the 35S promoter 3’-end and 15 nt of homology with the NOS terminator 5’-end. Moreover, it has the monocot Kozak consensus sequence AACC in front of the start codon. D . In-Fusion™ reaction and assembly of pAUrumII LIN or pAUrumIII LIN with gfp IFR and subsequent transformation of non-ligated construct to E. coli , where repair and ligation will occur. There are no unwanted nucleotides in the final construct.
    Figure Legend Snippet: Overview of SRL used with In-Fusion™ cloning. Red letters indicate recognition sites and black triangles cleaving sites of LguI and BaeI . Red arrows indicate where In-Fusion™ enzyme exonuclease activity will occur. A . Spacer-removal linearization (SRL) of pAUrumII (to pAUrumII LIN ) with type IIS RE LguI . The spacer holds the LacZα coding sequence with its promoter. B . SRL of pAUrumIII (to pAUrumIII LIN ) with type IIB RE BaeI . C . Extended primers used in PCR amplification of gfp . The In-Fusion™-ready product, gfp IFR , has 15 nt of homology with the 35S promoter 3’-end and 15 nt of homology with the NOS terminator 5’-end. Moreover, it has the monocot Kozak consensus sequence AACC in front of the start codon. D . In-Fusion™ reaction and assembly of pAUrumII LIN or pAUrumIII LIN with gfp IFR and subsequent transformation of non-ligated construct to E. coli , where repair and ligation will occur. There are no unwanted nucleotides in the final construct.

    Techniques Used: Clone Assay, Activity Assay, Sequencing, Polymerase Chain Reaction, Amplification, Transformation Assay, Construct, Ligation

    Related Articles

    Transfection:

    Article Title: Gluconeogenesis using glycerol as a substrate in bloodstream-form Trypanosoma brucei
    Article Snippet: .. The resulting pNATmNG THT1 was linearized with BaeI and pNATmNG THT2 with BsrGI (NEB) prior to transfection. .. The pRPaiSL vector [ ] was used to assemble the RNAi constructs.

    Amplification:

    Article Title: SLTAB2 is the paramutated SULFUREA locus in tomato
    Article Snippet: .. Genotyping of amplified cDNA was performed by digesting 100ng purified SLTAB2 amplicon with BaeI (NEB) for 12h at 25 °C in 1× NEB2.1, 100 µg ml−1 BSA, and 20 µM SAM as per the manufacturer’s instructions, and electrophoresis on a 1.5% agarose gel. .. Strand-specific RNA-Seq libraries for two wild-types and three pairs of sulf and sulf /+ were made and indexed with the ScriptSeq v2 kit (Epicentre) according to the protocol after RiboZero treatment (Plant leaf, Epicentre), and sequenced as a pool on one lane of HiSeq 2000 100PE.

    Agarose Gel Electrophoresis:

    Article Title: SLTAB2 is the paramutated SULFUREA locus in tomato
    Article Snippet: .. Genotyping of amplified cDNA was performed by digesting 100ng purified SLTAB2 amplicon with BaeI (NEB) for 12h at 25 °C in 1× NEB2.1, 100 µg ml−1 BSA, and 20 µM SAM as per the manufacturer’s instructions, and electrophoresis on a 1.5% agarose gel. .. Strand-specific RNA-Seq libraries for two wild-types and three pairs of sulf and sulf /+ were made and indexed with the ScriptSeq v2 kit (Epicentre) according to the protocol after RiboZero treatment (Plant leaf, Epicentre), and sequenced as a pool on one lane of HiSeq 2000 100PE.

    Article Title: Systematic metabolic engineering for improvement of glycosylation efficiency in Escherichia coli
    Article Snippet: .. Briefly, pACYCpgl was digested with BaeI (NEB, Herfordshire, UK) at 37 °C for 1 h. After confirmation by agarose gel electrophoresis, the linear DNA was digested with exonuclease BAL-31 (NEB) for 5, 10, 20 and 30 min at 30 °C. .. The reaction was stopped by addition of SureClean reagent (Bioline, London, UK).

    Purification:

    Article Title: Rapidly evolving protointrons in Saccharomyces genomes revealed by a hungry spliceosome
    Article Snippet: .. This duplex was purified and assembled using Gibson mix (NEB) with p426-crRNA-CAN1.Y that had been cut with NheI and Acc65I to replace the CAN1.Y guide target region with a stuffer fragment that could be released by BaeI (NEB) and allow any guide to be inserted easily (p426-crRNA-BaeI). ..

    Electrophoresis:

    Article Title: SLTAB2 is the paramutated SULFUREA locus in tomato
    Article Snippet: .. Genotyping of amplified cDNA was performed by digesting 100ng purified SLTAB2 amplicon with BaeI (NEB) for 12h at 25 °C in 1× NEB2.1, 100 µg ml−1 BSA, and 20 µM SAM as per the manufacturer’s instructions, and electrophoresis on a 1.5% agarose gel. .. Strand-specific RNA-Seq libraries for two wild-types and three pairs of sulf and sulf /+ were made and indexed with the ScriptSeq v2 kit (Epicentre) according to the protocol after RiboZero treatment (Plant leaf, Epicentre), and sequenced as a pool on one lane of HiSeq 2000 100PE.

    Plasmid Preparation:

    Article Title: Terminal protein-primed amplification of heterologous DNA with a minimal replication system based on phage ?29
    Article Snippet: .. Digestion of plasmid pEYFPORBae with BaeI was done in New England Biolabs Buffer 4 supplemented with 0.1 mg/mL of BSA and 20 μM S-adenosylmethionine. .. After digestion with BaeI, the DNA was purified by Qiaquick Spin columns (Qiagen).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    New England Biolabs baei
    A pETORPHI derivative linearized with <t>BaeI</t> is efficient in amplification. ( A ) Scheme of BaeI recognition (underlined) and cutting sites (arrows) and position of Φ29 origins (capital letters) in <t>pEYFPORBae.</t> ( B ) Plasmid pEYFPORBae was prepared as
    Baei, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/baei/product/New England Biolabs
    Average 93 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    baei - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

    Image Search Results


    A pETORPHI derivative linearized with BaeI is efficient in amplification. ( A ) Scheme of BaeI recognition (underlined) and cutting sites (arrows) and position of Φ29 origins (capital letters) in pEYFPORBae. ( B ) Plasmid pEYFPORBae was prepared as

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Terminal protein-primed amplification of heterologous DNA with a minimal replication system based on phage ?29

    doi: 10.1073/pnas.1114397108

    Figure Lengend Snippet: A pETORPHI derivative linearized with BaeI is efficient in amplification. ( A ) Scheme of BaeI recognition (underlined) and cutting sites (arrows) and position of Φ29 origins (capital letters) in pEYFPORBae. ( B ) Plasmid pEYFPORBae was prepared as

    Article Snippet: Digestion of plasmid pEYFPORBae with BaeI was done in New England Biolabs Buffer 4 supplemented with 0.1 mg/mL of BSA and 20 μM S-adenosylmethionine.

    Techniques: Amplification, Plasmid Preparation

    SLTAB2 paramutation in the cross between a heterozygous sulf /+ and S. pimpinellifolium . (A) Phenotypes of a paramutated (left) and wild-type leaf (right) in the F1. (B) SLTAB2 expression in the F1. Paramutated plants showed reduced expression in the green sectors compared with wt plants ( P =7.8e-4, two-tailed t test), and even further reduction in the chlorotic sectors ( P =6.6e-3, paired t test between green and yellow samples). Data are plotted on a log 2 scale, the mean is represented by a horizontal bar. Paired data points for paramutated plants (green and yellow sectors) are joined by grey lines. (C) Only the S. pimpinellifolium SLTAB2 allele is expressed in paramutated F1 plants. The S. lycopersicum allele is sensitive to digestion by the BaeI restriction enzyme, resulting in a smear. Two SNPs in the S. pimpinellifolium SLTAB2 allele make it resistant to BaeI treatment. F1s expressing the S. lycopersicum allele show a smear, whereas F1s in which the S. lycopersicum allele is silent do not.

    Journal: Journal of Experimental Botany

    Article Title: SLTAB2 is the paramutated SULFUREA locus in tomato

    doi: 10.1093/jxb/erw096

    Figure Lengend Snippet: SLTAB2 paramutation in the cross between a heterozygous sulf /+ and S. pimpinellifolium . (A) Phenotypes of a paramutated (left) and wild-type leaf (right) in the F1. (B) SLTAB2 expression in the F1. Paramutated plants showed reduced expression in the green sectors compared with wt plants ( P =7.8e-4, two-tailed t test), and even further reduction in the chlorotic sectors ( P =6.6e-3, paired t test between green and yellow samples). Data are plotted on a log 2 scale, the mean is represented by a horizontal bar. Paired data points for paramutated plants (green and yellow sectors) are joined by grey lines. (C) Only the S. pimpinellifolium SLTAB2 allele is expressed in paramutated F1 plants. The S. lycopersicum allele is sensitive to digestion by the BaeI restriction enzyme, resulting in a smear. Two SNPs in the S. pimpinellifolium SLTAB2 allele make it resistant to BaeI treatment. F1s expressing the S. lycopersicum allele show a smear, whereas F1s in which the S. lycopersicum allele is silent do not.

    Article Snippet: Genotyping of amplified cDNA was performed by digesting 100ng purified SLTAB2 amplicon with BaeI (NEB) for 12h at 25 °C in 1× NEB2.1, 100 µg ml−1 BSA, and 20 µM SAM as per the manufacturer’s instructions, and electrophoresis on a 1.5% agarose gel.

    Techniques: Expressing, Two Tailed Test

    Overview of SRL used with In-Fusion™ cloning. Red letters indicate recognition sites and black triangles cleaving sites of LguI and BaeI . Red arrows indicate where In-Fusion™ enzyme exonuclease activity will occur. A . Spacer-removal linearization (SRL) of pAUrumII (to pAUrumII LIN ) with type IIS RE LguI . The spacer holds the LacZα coding sequence with its promoter. B . SRL of pAUrumIII (to pAUrumIII LIN ) with type IIB RE BaeI . C . Extended primers used in PCR amplification of gfp . The In-Fusion™-ready product, gfp IFR , has 15 nt of homology with the 35S promoter 3’-end and 15 nt of homology with the NOS terminator 5’-end. Moreover, it has the monocot Kozak consensus sequence AACC in front of the start codon. D . In-Fusion™ reaction and assembly of pAUrumII LIN or pAUrumIII LIN with gfp IFR and subsequent transformation of non-ligated construct to E. coli , where repair and ligation will occur. There are no unwanted nucleotides in the final construct.

    Journal: Plant Methods

    Article Title: A novel approach to the generation of seamless constructs for plant transformation

    doi: 10.1186/1746-4811-10-10

    Figure Lengend Snippet: Overview of SRL used with In-Fusion™ cloning. Red letters indicate recognition sites and black triangles cleaving sites of LguI and BaeI . Red arrows indicate where In-Fusion™ enzyme exonuclease activity will occur. A . Spacer-removal linearization (SRL) of pAUrumII (to pAUrumII LIN ) with type IIS RE LguI . The spacer holds the LacZα coding sequence with its promoter. B . SRL of pAUrumIII (to pAUrumIII LIN ) with type IIB RE BaeI . C . Extended primers used in PCR amplification of gfp . The In-Fusion™-ready product, gfp IFR , has 15 nt of homology with the 35S promoter 3’-end and 15 nt of homology with the NOS terminator 5’-end. Moreover, it has the monocot Kozak consensus sequence AACC in front of the start codon. D . In-Fusion™ reaction and assembly of pAUrumII LIN or pAUrumIII LIN with gfp IFR and subsequent transformation of non-ligated construct to E. coli , where repair and ligation will occur. There are no unwanted nucleotides in the final construct.

    Article Snippet: In another 100 μL reaction solution containing 20 μM S-adenosyl methionine, 5 μg pAUrumIII was digested 15 h at 25°C using 10 U/μg of the type IIB RE BaeI (New England Biolabs).

    Techniques: Clone Assay, Activity Assay, Sequencing, Polymerase Chain Reaction, Amplification, Transformation Assay, Construct, Ligation