Journal: Frontiers in Microbiology
Article Title: Inhibition of Xanthomonas fragariae, Causative Agent of Angular Leaf Spot of Strawberry, through Iron Deprivation
Figure Lengend Snippet: Btg I-based identification of X. fragariae . (A) : alignment of partial 16 s rRNA gene sequences from Xanthomonas type strains, showing the unique cytosine (bold and capitalized) and Btg I recognition site (underlined) for X. fragariae . (B) : banding pattern following Btg I-digestion of pA-518r amplicons from the following bacterial strains: 1–16: Fa P21– Fa P36, 17: X. campestris Fa P1, 18: P. putida 1290, 19: Collimonas fungivorans Ter331.
Article Snippet: PCR was performed using 16S rRNA gene universal primers pA and 1492r ( ) or 518r ( ) and sent for sequencing at the UC Davis DNA sequencing was done at the UC Davis DNA Sequencing Facility using BigDye® Terminator v3.1 Cycle Sequencing Kit with The Gel Company’s Better Buffer, or digested with Btg I, following instructions of the manufacturer (New England Biolabs).