btgi  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Name:
    BtgI
    Description:
    BtgI 1 000 units
    Catalog Number:
    R0608L
    Price:
    65
    Category:
    Restriction Enzymes
    Size:
    1 000 units
    Buy from Supplier


    Structured Review

    New England Biolabs btgi
    BtgI
    BtgI 1 000 units
    https://www.bioz.com/result/btgi/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    btgi - by Bioz Stars, 2021-06
    93/100 stars

    Images

    1) Product Images from "Association of Novel and Highly Diverse Acid-Tolerant Denitrifiers with N2O Fluxes of an Acidic Fen ▿O Fluxes of an Acidic Fen ▿ †"

    Article Title: Association of Novel and Highly Diverse Acid-Tolerant Denitrifiers with N2O Fluxes of an Acidic Fen ▿O Fluxes of an Acidic Fen ▿ †

    Journal: Applied and Environmental Microbiology

    doi: 10.1128/AEM.02256-09

    Comparative TRFLP analyses of narG (A to C) and nosZ (D to F) amplified from different soil layers of the acidic fen. PCR products were digested with CfoI (A), HaeIII (B), XhoI (C), BtgI (D), NlaIV (E), and PvuI and SacI (F). Mean values of three replicates
    Figure Legend Snippet: Comparative TRFLP analyses of narG (A to C) and nosZ (D to F) amplified from different soil layers of the acidic fen. PCR products were digested with CfoI (A), HaeIII (B), XhoI (C), BtgI (D), NlaIV (E), and PvuI and SacI (F). Mean values of three replicates

    Techniques Used: Terminal Restriction Fragment Length Polymorphism, Amplification, Polymerase Chain Reaction

    Related Articles

    Sequencing:

    Article Title: Interleukin 32 (IL-32) Contains a Typical ?-Helix Bundle Structure That Resembles Focal Adhesion Targeting Region of Focal Adhesion Kinase-1 *
    Article Snippet: The RGD deletion mutants were produced by digesting the pCDNA3-IL-32β/IL-32γ expression plasmids with HindIII (New England Biolabs, Ipswich, MA) and XbaI (New England Biolabs) to separate the backbone and insert. .. The inserts were digested with BtgI (New England Biolabs) to remove the GD amino acid sequence until the stop codon. .. Subsequently, the modified IL-32β/IL-32γ inserts were ligated at the HindIII overhang into the previously isolated backbone, which also contained a HindIII overhang, by using T4 DNA ligase (New England Biolabs).

    Article Title: RNA editing of BFP, a point mutant of GFP, using artificial APOBEC1 deaminase to restore the genetic code
    Article Snippet: 100 ng of cDNA was used for each PCR reaction, the total reaction volume was 20 ul. .. After the PCR amplification 10 ul of PCR product was taken for restriction digestion, where incubation was done at 37 °C for 2 h with BtgI (New England BioLabs) restriction enzyme, which cleaved the BFP sequence into two fragments of 201 and 123 bp but left the restored GFP sequence undigested. .. During the gel electrophoresis equal volume (2 ul) of digested product was loaded into the 14 well comb.

    Article Title: Inhibition of Xanthomonas fragariae, Causative Agent of Angular Leaf Spot of Strawberry, through Iron Deprivation
    Article Snippet: .. PCR was performed using 16S rRNA gene universal primers pA and 1492r ( ) or 518r ( ) and sent for sequencing at the UC Davis DNA sequencing was done at the UC Davis DNA Sequencing Facility using BigDye® Terminator v3.1 Cycle Sequencing Kit with The Gel Company’s Better Buffer, or digested with Btg I, following instructions of the manufacturer (New England Biolabs). .. For suspected X. fragariae strains, we also performed Multi Locus Sequence Analysis (MLSA) of the fyuA, gyrB, rpoD , and 16s rRNA genes ( ) and PCR confirmation using X. fragariae -specific PCR primer sets q241, q245, and q295 ( ).

    Polymerase Chain Reaction:

    Article Title: Association of Novel and Highly Diverse Acid-Tolerant Denitrifiers with N2O Fluxes of an Acidic Fen ▿O Fluxes of an Acidic Fen ▿ †
    Article Snippet: Digested DNA was purified using a QIAquick PCR Purification Kit (Qiagen GmbH, Hilden, Germany). .. PCR products of narG were digested with the restriction enzymes CfoI, HaeIII, or XhoI (New England Biolabs, Frankfurt am Main, Germany), and PCR products of nosZ were digested with BtgI, NlaIV, or PvuI plus SacI (New England Biolabs, Frankfurt am Main, Germany) (double digest). .. Gel electrophoresis was performed with an NEN model 4300 DNA analyzer (Licor, Lincoln, NE).

    Article Title: RNA editing of BFP, a point mutant of GFP, using artificial APOBEC1 deaminase to restore the genetic code
    Article Snippet: 100 ng of cDNA was used for each PCR reaction, the total reaction volume was 20 ul. .. After the PCR amplification 10 ul of PCR product was taken for restriction digestion, where incubation was done at 37 °C for 2 h with BtgI (New England BioLabs) restriction enzyme, which cleaved the BFP sequence into two fragments of 201 and 123 bp but left the restored GFP sequence undigested. .. During the gel electrophoresis equal volume (2 ul) of digested product was loaded into the 14 well comb.

    Article Title: Genetic analysis of polymorphisms in the kalirin gene for association with age-at-onset in European Huntington disease patients
    Article Snippet: Five μl of the PCR product were electrophoresed on 2% agarose gels and only samples with positive signals were used, yielding the total of 680 HD samples. .. The PCR products were incubated with 3U Alu I (rs10934657, rs111472457, rs61746078, rs2289838, rs2289838), 2.5U Btg I (rs35057827), 1.9U Msc I (rs13074913), 3U BamH I (rs61745397), 3U Spe I (rs112304715), 3U Nde I (rs2289838) or 3U Sac I (rs1062749) according to the manufacturer’s instructions (New England Biolabs, Inc., Beverly, MA, USA). ..

    Article Title: Inhibition of Xanthomonas fragariae, Causative Agent of Angular Leaf Spot of Strawberry, through Iron Deprivation
    Article Snippet: .. PCR was performed using 16S rRNA gene universal primers pA and 1492r ( ) or 518r ( ) and sent for sequencing at the UC Davis DNA sequencing was done at the UC Davis DNA Sequencing Facility using BigDye® Terminator v3.1 Cycle Sequencing Kit with The Gel Company’s Better Buffer, or digested with Btg I, following instructions of the manufacturer (New England Biolabs). .. For suspected X. fragariae strains, we also performed Multi Locus Sequence Analysis (MLSA) of the fyuA, gyrB, rpoD , and 16s rRNA genes ( ) and PCR confirmation using X. fragariae -specific PCR primer sets q241, q245, and q295 ( ).

    Amplification:

    Article Title: RNA editing of BFP, a point mutant of GFP, using artificial APOBEC1 deaminase to restore the genetic code
    Article Snippet: 100 ng of cDNA was used for each PCR reaction, the total reaction volume was 20 ul. .. After the PCR amplification 10 ul of PCR product was taken for restriction digestion, where incubation was done at 37 °C for 2 h with BtgI (New England BioLabs) restriction enzyme, which cleaved the BFP sequence into two fragments of 201 and 123 bp but left the restored GFP sequence undigested. .. During the gel electrophoresis equal volume (2 ul) of digested product was loaded into the 14 well comb.

    Incubation:

    Article Title: RNA editing of BFP, a point mutant of GFP, using artificial APOBEC1 deaminase to restore the genetic code
    Article Snippet: 100 ng of cDNA was used for each PCR reaction, the total reaction volume was 20 ul. .. After the PCR amplification 10 ul of PCR product was taken for restriction digestion, where incubation was done at 37 °C for 2 h with BtgI (New England BioLabs) restriction enzyme, which cleaved the BFP sequence into two fragments of 201 and 123 bp but left the restored GFP sequence undigested. .. During the gel electrophoresis equal volume (2 ul) of digested product was loaded into the 14 well comb.

    Article Title: Genetic analysis of polymorphisms in the kalirin gene for association with age-at-onset in European Huntington disease patients
    Article Snippet: Five μl of the PCR product were electrophoresed on 2% agarose gels and only samples with positive signals were used, yielding the total of 680 HD samples. .. The PCR products were incubated with 3U Alu I (rs10934657, rs111472457, rs61746078, rs2289838, rs2289838), 2.5U Btg I (rs35057827), 1.9U Msc I (rs13074913), 3U BamH I (rs61745397), 3U Spe I (rs112304715), 3U Nde I (rs2289838) or 3U Sac I (rs1062749) according to the manufacturer’s instructions (New England Biolabs, Inc., Beverly, MA, USA). ..

    DNA Sequencing:

    Article Title: Inhibition of Xanthomonas fragariae, Causative Agent of Angular Leaf Spot of Strawberry, through Iron Deprivation
    Article Snippet: .. PCR was performed using 16S rRNA gene universal primers pA and 1492r ( ) or 518r ( ) and sent for sequencing at the UC Davis DNA sequencing was done at the UC Davis DNA Sequencing Facility using BigDye® Terminator v3.1 Cycle Sequencing Kit with The Gel Company’s Better Buffer, or digested with Btg I, following instructions of the manufacturer (New England Biolabs). .. For suspected X. fragariae strains, we also performed Multi Locus Sequence Analysis (MLSA) of the fyuA, gyrB, rpoD , and 16s rRNA genes ( ) and PCR confirmation using X. fragariae -specific PCR primer sets q241, q245, and q295 ( ).

    Generated:

    Article Title: Plant A20/AN1 protein serves as the important hub to mediate antiviral immunity
    Article Snippet: The overnight yeast culture was diluted to an OD600 of 0.06 and spotted on selection plates (containing histidine- , leucine- , tryptophan- and 5-bromo-4-chloro-3-indolyl-alpha-D-galactopyranoside) for growth assay. .. Fragments of different N-terminus deletions of Pha13 were generated with restriction enzyme digestions of FseI (NEB), ApaI (NEB), Sgr AI (NEB), BtgI (NEB), or PspXI (NEB). .. Fragments of C-terminus deletions were generated by PCR amplification using primer pairs, Nd13Fs412F/ Eco RI-Pha13-R, Nd13Ap361F/ Eco RI-Pha13-R, Nd13Sg247F/ Eco RI-Pha13-R, Nd13Bt136F /Eco RI-Pha13-R, and Nd13Ps88F /Eco RI-Pha13-R ( ).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    New England Biolabs btg i
    <t>Btg</t> I-based identification of X. fragariae . (A) : alignment of partial 16 s rRNA gene sequences from Xanthomonas type strains, showing the unique cytosine (bold and capitalized) and <t>Btg</t> I recognition site (underlined) for X. fragariae . (B) : banding pattern following Btg I-digestion of pA-518r amplicons from the following bacterial strains: 1–16: Fa P21– Fa P36, 17: X. campestris Fa P1, 18: P. putida 1290, 19: Collimonas fungivorans Ter331.
    Btg I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/btg i/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    btg i - by Bioz Stars, 2021-06
    93/100 stars
      Buy from Supplier

    Image Search Results


    Btg I-based identification of X. fragariae . (A) : alignment of partial 16 s rRNA gene sequences from Xanthomonas type strains, showing the unique cytosine (bold and capitalized) and Btg I recognition site (underlined) for X. fragariae . (B) : banding pattern following Btg I-digestion of pA-518r amplicons from the following bacterial strains: 1–16: Fa P21– Fa P36, 17: X. campestris Fa P1, 18: P. putida 1290, 19: Collimonas fungivorans Ter331.

    Journal: Frontiers in Microbiology

    Article Title: Inhibition of Xanthomonas fragariae, Causative Agent of Angular Leaf Spot of Strawberry, through Iron Deprivation

    doi: 10.3389/fmicb.2016.01589

    Figure Lengend Snippet: Btg I-based identification of X. fragariae . (A) : alignment of partial 16 s rRNA gene sequences from Xanthomonas type strains, showing the unique cytosine (bold and capitalized) and Btg I recognition site (underlined) for X. fragariae . (B) : banding pattern following Btg I-digestion of pA-518r amplicons from the following bacterial strains: 1–16: Fa P21– Fa P36, 17: X. campestris Fa P1, 18: P. putida 1290, 19: Collimonas fungivorans Ter331.

    Article Snippet: PCR was performed using 16S rRNA gene universal primers pA and 1492r ( ) or 518r ( ) and sent for sequencing at the UC Davis DNA sequencing was done at the UC Davis DNA Sequencing Facility using BigDye® Terminator v3.1 Cycle Sequencing Kit with The Gel Company’s Better Buffer, or digested with Btg I, following instructions of the manufacturer (New England Biolabs).

    Techniques:

    Comparative TRFLP analyses of narG (A to C) and nosZ (D to F) amplified from different soil layers of the acidic fen. PCR products were digested with CfoI (A), HaeIII (B), XhoI (C), BtgI (D), NlaIV (E), and PvuI and SacI (F). Mean values of three replicates

    Journal: Applied and Environmental Microbiology

    Article Title: Association of Novel and Highly Diverse Acid-Tolerant Denitrifiers with N2O Fluxes of an Acidic Fen ▿O Fluxes of an Acidic Fen ▿ †

    doi: 10.1128/AEM.02256-09

    Figure Lengend Snippet: Comparative TRFLP analyses of narG (A to C) and nosZ (D to F) amplified from different soil layers of the acidic fen. PCR products were digested with CfoI (A), HaeIII (B), XhoI (C), BtgI (D), NlaIV (E), and PvuI and SacI (F). Mean values of three replicates

    Article Snippet: PCR products of narG were digested with the restriction enzymes CfoI, HaeIII, or XhoI (New England Biolabs, Frankfurt am Main, Germany), and PCR products of nosZ were digested with BtgI, NlaIV, or PvuI plus SacI (New England Biolabs, Frankfurt am Main, Germany) (double digest).

    Techniques: Terminal Restriction Fragment Length Polymorphism, Amplification, Polymerase Chain Reaction