sfoi  (New England Biolabs)


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  • 93
    Name:
    SfoI
    Description:
    SfoI 2 500 units
    Catalog Number:
    r0606l
    Price:
    269
    Category:
    Restriction Enzymes
    Size:
    2 500 units
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    Structured Review

    New England Biolabs sfoi
    SfoI
    SfoI 2 500 units
    https://www.bioz.com/result/sfoi/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sfoi - by Bioz Stars, 2021-03
    93/100 stars

    Images

    1) Product Images from "One recognition sequence, seven restriction enzymes, five reaction mechanisms"

    Article Title: One recognition sequence, seven restriction enzymes, five reaction mechanisms

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkh685

    Enzymes and substrates. ( A ) Shown, within the grey box, are the sequence GGCGCC and the sequences flanking this site in pUC19. The enzymes KasI, NarI, Mly113I, SfoI, EgeI, EheI and BbeI cleave GGCGCC at the positions shown. ( B ) The plasmids pUC19 (2686
    Figure Legend Snippet: Enzymes and substrates. ( A ) Shown, within the grey box, are the sequence GGCGCC and the sequences flanking this site in pUC19. The enzymes KasI, NarI, Mly113I, SfoI, EgeI, EheI and BbeI cleave GGCGCC at the positions shown. ( B ) The plasmids pUC19 (2686

    Techniques Used: Sequencing

    Related Articles

    Polymerase Chain Reaction:

    Article Title: sox2 and sox3 cooperate to regulate otic/epibranchial placode induction in zebrafish
    Article Snippet: DNA from single embryos was extracted following methods described previously , with addition of a proteinase-K digestion step for embryos older than 12 hpf or fixed embryos processed by in situ hybridization. sox2 homozygous and heterozygous mutants were identified by PCR using forward primer 5′-CCAGCAAAGTTACCTCCAACTG-3′ and reverse primer 5′-GCAGGGTGTACTTGTCCTTCTT-3′. .. PCR products were then digested with restriction enzyme SfoI or NarI (NEB), yielding wild-type fragments of 330 and 160 bp, while the mutant PCR product remains uncut at 490 bp. .. To identify sox3x52 mutants, indel-PCR was performed using three primers in a single reaction: forward primer-1 5′-CGTTTTCTTTCGAGTGCTTGGC-3′, forward primer-2 (indel primer) 5′-GCAAAAACAACAGTGCCAACGA-3′ and reverse primer 5′-TTTGTAATCCGGGTGCTCCTTC-3′. sox3x52/x52 homozygous mutant DNA yielded a single 344 bp amplicon, while wild-type or sox3x52/+ heterozygous DNA yielded 239 and 344 bp fragments.

    Article Title: Rational design of novel fluorescent enzyme biosensors for direct detection of strigolactones
    Article Snippet: LexA-DAD2, B42AD-MAX2 and PLexA were PCR amplified using primers 105+106, 109+110, and 113+124, respectively. .. All PCR fragments were purified using EasyPure ® PCR Purification Kit (TransGen Biotech) as per the manufacturers protocol and co-transformed into CEN.PK2-1C with pRS425 episomal plasmid backbone digested with SfoI and SacI (NEB). .. The assembled plasmid was extracted from S. cerevisiae, propagated in E. coli and verified by sequencing.

    Mutagenesis:

    Article Title: sox2 and sox3 cooperate to regulate otic/epibranchial placode induction in zebrafish
    Article Snippet: DNA from single embryos was extracted following methods described previously , with addition of a proteinase-K digestion step for embryos older than 12 hpf or fixed embryos processed by in situ hybridization. sox2 homozygous and heterozygous mutants were identified by PCR using forward primer 5′-CCAGCAAAGTTACCTCCAACTG-3′ and reverse primer 5′-GCAGGGTGTACTTGTCCTTCTT-3′. .. PCR products were then digested with restriction enzyme SfoI or NarI (NEB), yielding wild-type fragments of 330 and 160 bp, while the mutant PCR product remains uncut at 490 bp. .. To identify sox3x52 mutants, indel-PCR was performed using three primers in a single reaction: forward primer-1 5′-CGTTTTCTTTCGAGTGCTTGGC-3′, forward primer-2 (indel primer) 5′-GCAAAAACAACAGTGCCAACGA-3′ and reverse primer 5′-TTTGTAATCCGGGTGCTCCTTC-3′. sox3x52/x52 homozygous mutant DNA yielded a single 344 bp amplicon, while wild-type or sox3x52/+ heterozygous DNA yielded 239 and 344 bp fragments.

    Transformation Assay:

    Article Title: Assessment of Gut Bacteria for a Paratransgenic Approach To Control Dermolepida albohirtum Larvae
    Article Snippet: DNA was extracted from 4 ml of each colony by use of a phenol-chloroform method , followed by PCR detection of pBBR by using primers 3892F and 4944R, and plasmid was extracted from 5 ml of each colony by using a QIAprep Spin miniprep kit (Qiagen) following the manufacturer's protocol. .. Control pBBR and plasmids extracted from transformed colonies were digested using SfoI (NEB) or RsaI (NEB) and run in a 1% agarose gel for comparison. .. DH5α was electroporated with plasmid isolated from a Da-11 colony that was previously transformed with pBBR and grown on plates containing kanamycin.

    Agarose Gel Electrophoresis:

    Article Title: Assessment of Gut Bacteria for a Paratransgenic Approach To Control Dermolepida albohirtum Larvae
    Article Snippet: DNA was extracted from 4 ml of each colony by use of a phenol-chloroform method , followed by PCR detection of pBBR by using primers 3892F and 4944R, and plasmid was extracted from 5 ml of each colony by using a QIAprep Spin miniprep kit (Qiagen) following the manufacturer's protocol. .. Control pBBR and plasmids extracted from transformed colonies were digested using SfoI (NEB) or RsaI (NEB) and run in a 1% agarose gel for comparison. .. DH5α was electroporated with plasmid isolated from a Da-11 colony that was previously transformed with pBBR and grown on plates containing kanamycin.

    Plasmid Preparation:

    Article Title: The Structure of the TNFRSF13C Promoter Enables Differential Expression of BAFF-R during B Cell Ontogeny and Terminal Differentiation
    Article Snippet: Probes were generated by digesting the BAFF-R putative promoter-containing pGL3-Basic vector with various restriction enzymes. .. Probe 1 (−289 — +33) and Probe 2 (−722 — −239) were cut from the plasmid with enzyme pairs NcoI and SfoI and EcoRV and XmaI (NEBiolabs), respectively, to generate 5′overhangs to be labeled by filling in with radioactive nucleotides. .. After 10 pmol of plasmid were digested for 2 h at 37°C in NEB Buffer 4, the probes were labeled by the addition of Klenow, 100 μCi of 32 P-labeled dCTP, and an excess of unlabeled dATP, dTTP, and dGTP.

    Article Title: Catabolite and Oxygen Regulation of Enterohemorrhagic Escherichia coli Virulence
    Article Snippet: .. To construct the complement vectors for each single gene in pACYC184, the plasmid was digested with NEB enzymes EcoRV and SalI. pKCB04 was digested with SfoI and SmaI, pKCB05 was digested with SfoI and SalI, and pKCB06 was digested with SfoI and SalI (NEB). .. The digested template and respective genes were gel purified (Qiagen) before ligation with NEB T4 ligase overnight at 16°C.

    Article Title: Rational design of novel fluorescent enzyme biosensors for direct detection of strigolactones
    Article Snippet: LexA-DAD2, B42AD-MAX2 and PLexA were PCR amplified using primers 105+106, 109+110, and 113+124, respectively. .. All PCR fragments were purified using EasyPure ® PCR Purification Kit (TransGen Biotech) as per the manufacturers protocol and co-transformed into CEN.PK2-1C with pRS425 episomal plasmid backbone digested with SfoI and SacI (NEB). .. The assembled plasmid was extracted from S. cerevisiae, propagated in E. coli and verified by sequencing.

    Labeling:

    Article Title: The Structure of the TNFRSF13C Promoter Enables Differential Expression of BAFF-R during B Cell Ontogeny and Terminal Differentiation
    Article Snippet: Probes were generated by digesting the BAFF-R putative promoter-containing pGL3-Basic vector with various restriction enzymes. .. Probe 1 (−289 — +33) and Probe 2 (−722 — −239) were cut from the plasmid with enzyme pairs NcoI and SfoI and EcoRV and XmaI (NEBiolabs), respectively, to generate 5′overhangs to be labeled by filling in with radioactive nucleotides. .. After 10 pmol of plasmid were digested for 2 h at 37°C in NEB Buffer 4, the probes were labeled by the addition of Klenow, 100 μCi of 32 P-labeled dCTP, and an excess of unlabeled dATP, dTTP, and dGTP.

    Sequencing:

    Article Title: Human Hepatocyte Nuclear Factor 4-α Encodes Isoforms with Distinct Transcriptional Functions.
    Article Snippet: - Inserting the C-Termini into pENTR11 The three different C-terminal ends of the HNF4α isoforms, lined with a 3 'XhoI restriction site, were synthesized directly as double-stranded DNA (gBlocks Gene Fragments) (IDT, San Jose, USA). .. These three sequences as well as the four pENTR11 plasmids containing the different Nterminal ends in front of the common HNF4α sequence were digested by the restriction enzymes SfoI (only for the plasmids) and XhoI (New England Biolabs, Ipswich, USA) in the CutSmart buffer for 2 hours at 37°C, then gel purified. .. A ligation reaction as described previously was subsequently performed to insert each C-terminus downstream of the common HNF4α sequence into pENTR11.

    Purification:

    Article Title: Human Hepatocyte Nuclear Factor 4-α Encodes Isoforms with Distinct Transcriptional Functions.
    Article Snippet: - Inserting the C-Termini into pENTR11 The three different C-terminal ends of the HNF4α isoforms, lined with a 3 'XhoI restriction site, were synthesized directly as double-stranded DNA (gBlocks Gene Fragments) (IDT, San Jose, USA). .. These three sequences as well as the four pENTR11 plasmids containing the different Nterminal ends in front of the common HNF4α sequence were digested by the restriction enzymes SfoI (only for the plasmids) and XhoI (New England Biolabs, Ipswich, USA) in the CutSmart buffer for 2 hours at 37°C, then gel purified. .. A ligation reaction as described previously was subsequently performed to insert each C-terminus downstream of the common HNF4α sequence into pENTR11.

    Article Title: Rational design of novel fluorescent enzyme biosensors for direct detection of strigolactones
    Article Snippet: LexA-DAD2, B42AD-MAX2 and PLexA were PCR amplified using primers 105+106, 109+110, and 113+124, respectively. .. All PCR fragments were purified using EasyPure ® PCR Purification Kit (TransGen Biotech) as per the manufacturers protocol and co-transformed into CEN.PK2-1C with pRS425 episomal plasmid backbone digested with SfoI and SacI (NEB). .. The assembled plasmid was extracted from S. cerevisiae, propagated in E. coli and verified by sequencing.

    Clone Assay:

    Article Title: Efficient enrichment cloning of TAL effector genes from Xanthomonas
    Article Snippet: Plasmid DNA wase extracted using QIAprep Spin Miniprep Kit or QIAGEN Plasmid Kit for midiprep (Qiagen SAS). .. Enrichment cloning of tal gene BamHI fragments 20 μg of genomic DNA were digested with BamHI-HF, SfoI and ApaLI in CutSmart® Buffer (New England Biolabs SAS, Enry, France) at 37 °C overnight. .. The High-Fidelity version of BamHI was used to avoid star activities upon prolonged incubation.

    Construct:

    Article Title: Catabolite and Oxygen Regulation of Enterohemorrhagic Escherichia coli Virulence
    Article Snippet: .. To construct the complement vectors for each single gene in pACYC184, the plasmid was digested with NEB enzymes EcoRV and SalI. pKCB04 was digested with SfoI and SmaI, pKCB05 was digested with SfoI and SalI, and pKCB06 was digested with SfoI and SalI (NEB). .. The digested template and respective genes were gel purified (Qiagen) before ligation with NEB T4 ligase overnight at 16°C.

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    New England Biolabs sfoi
    Enzymes and substrates. ( A ) Shown, within the grey box, are the sequence GGCGCC and the sequences flanking this site in pUC19. The enzymes KasI, NarI, Mly113I, <t>SfoI,</t> <t>EgeI,</t> EheI and BbeI cleave GGCGCC at the positions shown. ( B ) The plasmids pUC19 (2686
    Sfoi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sfoi/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sfoi - by Bioz Stars, 2021-03
    93/100 stars
      Buy from Supplier

    Image Search Results


    Enzymes and substrates. ( A ) Shown, within the grey box, are the sequence GGCGCC and the sequences flanking this site in pUC19. The enzymes KasI, NarI, Mly113I, SfoI, EgeI, EheI and BbeI cleave GGCGCC at the positions shown. ( B ) The plasmids pUC19 (2686

    Journal: Nucleic Acids Research

    Article Title: One recognition sequence, seven restriction enzymes, five reaction mechanisms

    doi: 10.1093/nar/gkh685

    Figure Lengend Snippet: Enzymes and substrates. ( A ) Shown, within the grey box, are the sequence GGCGCC and the sequences flanking this site in pUC19. The enzymes KasI, NarI, Mly113I, SfoI, EgeI, EheI and BbeI cleave GGCGCC at the positions shown. ( B ) The plasmids pUC19 (2686

    Article Snippet: Restriction enzymes were obtained from the following suppliers and stored at −20°C: BbeI, Takara Biomedicals, Japan; EgeI and Mly113I, SibEnzyme, Russia; EheI, MBI Fermentas, Lithuania; KasI, NarI and SfoI, New England Biolabs, USA.

    Techniques: Sequencing

    Genetic targeting of sox2 and sox3 (A) Sequences for targeting vectors for sox2 (TALEN) and sox3 (sgRNA) and resulting lesions in sox2 (allele x50 ) and sox3 (alleles x52 and x53 ). The deletion in x50 leads to loss of an SfoI restriction site (magenta), which was used for genotyping, whereas sox3 indels were identified by allele-specific PCR primers (see Materials Methods). (B–L) Live embryos at 26 hpf showing general morphology (B–D), cranial development (E–G), tail development (K–L) and the otic vesicle (H–J, lateral views with anterior to the left; Scale bar, 50 μm) in control, sox2 −/ − and sox3 −/ − mutants. Excess cells at the ventral tip of the tail in sox2 −/ − embryos are indicated (arrow). (M) Box-and-whisker plot of surface area of otic vesicle, normalized to control embryos, in control embryos and sox2 −/ − and sox3 −/ − mutants. Green line indicates mean. Asterisk indicates statistically significant difference relative to control embryos ( *** P

    Journal: Developmental biology

    Article Title: sox2 and sox3 cooperate to regulate otic/epibranchial placode induction in zebrafish

    doi: 10.1016/j.ydbio.2018.01.011

    Figure Lengend Snippet: Genetic targeting of sox2 and sox3 (A) Sequences for targeting vectors for sox2 (TALEN) and sox3 (sgRNA) and resulting lesions in sox2 (allele x50 ) and sox3 (alleles x52 and x53 ). The deletion in x50 leads to loss of an SfoI restriction site (magenta), which was used for genotyping, whereas sox3 indels were identified by allele-specific PCR primers (see Materials Methods). (B–L) Live embryos at 26 hpf showing general morphology (B–D), cranial development (E–G), tail development (K–L) and the otic vesicle (H–J, lateral views with anterior to the left; Scale bar, 50 μm) in control, sox2 −/ − and sox3 −/ − mutants. Excess cells at the ventral tip of the tail in sox2 −/ − embryos are indicated (arrow). (M) Box-and-whisker plot of surface area of otic vesicle, normalized to control embryos, in control embryos and sox2 −/ − and sox3 −/ − mutants. Green line indicates mean. Asterisk indicates statistically significant difference relative to control embryos ( *** P

    Article Snippet: PCR products were then digested with restriction enzyme SfoI or NarI (NEB), yielding wild-type fragments of 330 and 160 bp, while the mutant PCR product remains uncut at 490 bp.

    Techniques: Polymerase Chain Reaction, Whisker Assay