sgrai  (New England Biolabs)


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  • 93
    Name:
    SgrAI
    Description:
    SgrAI 5 000 units
    Catalog Number:
    R0603L
    Price:
    282
    Category:
    Restriction Enzymes
    Size:
    5 000 units
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    Structured Review

    New England Biolabs sgrai
    SgrAI
    SgrAI 5 000 units
    https://www.bioz.com/result/sgrai/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sgrai - by Bioz Stars, 2021-05
    93/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: In vitro characterization of 6S RNA release-defective mutants uncovers features of pRNA-dependent release from RNA polymerase in E. coli
    Article Snippet: The three different DNA products were cloned into the pEcoli-Cterm 6xHN (Clontech) vector as described below (Supplemental Fig. S9). .. The DNA products for T1, R9-33, and LowBinder along with pEcoli-Cterm 6xHN vector were double-digested with ClaI and SgrAI (NEB) . .. The vector was treated with CIAP (Roche), and ligation was carried out withT4 DNA ligase (Invitrogen) following the supplier's recommendations.

    Article Title: A Novel Mechanism for Nicotinic Potentiation of Glutamatergic Synapses
    Article Snippet: .. The super-ecliptic pHluorin (SEP)-tagged GluA1 construct (FUGW-GluA1-SEP) was made by removing green fluorescent protein (GFP) from FUGW (plasmid 14883; Addgene ; ) with the restriction enzymes AgeI and BsrG1 (New England BioLabs) and inserting the PCR-amplified GluA1-SEP from pCI-GluA1-SEP with SgrAI- and BsiWI-cut (New England BioLabs) sticky ends. .. Sequence integrity for the GluA1-SEP was confirmed using primer-walk sequencing (Integrated DNA Technologies).

    Article Title: Features of the Influence of a DNA Sequence on Its Adjacent Sequence
    Article Snippet: After purification, the concentrations of the digested DNA fragments a , c , g , and t were 6.9, 6.6, 6.9, and 6.6 ng/μL, respectively, which were determined as mentioned above. .. Digestion of the Plasmid pET-42a (+) 1 The digestion reaction: The plasmid pET-42a (+) was digested with SgrAI (NEB, USA) and QuickCut Xba I and Sph I (Takara Bio, China) following the manufacturer’s instruction. .. Briefly, the plasmid pET-42a (+) was digested in 50 μL of digestion reaction mixture containing 1× NEB buffer, 820 ng of plasmid pET-42a (+), 1 μL of each of Sph I and Xba I, and 2 U of SgrAI.

    Sequencing:

    Article Title: Self-generated DNA termini relax the specificity of SgrAI restriction endonuclease
    Article Snippet: .. The last unique site, CGCCGGCC, which was not present on the above tested DNA substrates, was determined to be cleaved by Sgr AI by using a derivative of pBR322 that contained two copies of this sequence within the 3.0-kb Pseudomonas alcaligenes genomic DNA insert (R. Vaisvila, New England Biolabs). .. Cleavage of either Ad2 or λ DNA also yielded a few fragments that were inconsistent with the computer-derived mapping data for the sequence CPuCCGGPyN, however.

    Polymerase Chain Reaction:

    Article Title: Engineering Foot-and-Mouth Disease Viruses with Improved Growth Properties for Vaccine Development
    Article Snippet: The PCR amplification thermal program to produce single-stranded PCR product consisted of an initial denaturation step at 94°C for 2 min, followed by 35 cycles of 94°C for 30 s, 57°C for 30 s, and 72°C for 3 min. .. This was followed by a final extension of 72°C for 10 min. Then, the PCR product was purified with the use of the Wizard SV Gel and PCR Clean-Up System Kit (Promega, Shanghai, China) and treated with the restriction endonucleases, Afl II and SgrA I (New England Biolabs, Massachusetts, USA). .. Meanwhile, the pO-FMDV plasmid was digested with the same restriction endonucleases.

    Article Title: A Novel Mechanism for Nicotinic Potentiation of Glutamatergic Synapses
    Article Snippet: .. The super-ecliptic pHluorin (SEP)-tagged GluA1 construct (FUGW-GluA1-SEP) was made by removing green fluorescent protein (GFP) from FUGW (plasmid 14883; Addgene ; ) with the restriction enzymes AgeI and BsrG1 (New England BioLabs) and inserting the PCR-amplified GluA1-SEP from pCI-GluA1-SEP with SgrAI- and BsiWI-cut (New England BioLabs) sticky ends. .. Sequence integrity for the GluA1-SEP was confirmed using primer-walk sequencing (Integrated DNA Technologies).

    Purification:

    Article Title: Engineering Foot-and-Mouth Disease Viruses with Improved Growth Properties for Vaccine Development
    Article Snippet: The PCR amplification thermal program to produce single-stranded PCR product consisted of an initial denaturation step at 94°C for 2 min, followed by 35 cycles of 94°C for 30 s, 57°C for 30 s, and 72°C for 3 min. .. This was followed by a final extension of 72°C for 10 min. Then, the PCR product was purified with the use of the Wizard SV Gel and PCR Clean-Up System Kit (Promega, Shanghai, China) and treated with the restriction endonucleases, Afl II and SgrA I (New England Biolabs, Massachusetts, USA). .. Meanwhile, the pO-FMDV plasmid was digested with the same restriction endonucleases.

    Construct:

    Article Title: A Novel Mechanism for Nicotinic Potentiation of Glutamatergic Synapses
    Article Snippet: .. The super-ecliptic pHluorin (SEP)-tagged GluA1 construct (FUGW-GluA1-SEP) was made by removing green fluorescent protein (GFP) from FUGW (plasmid 14883; Addgene ; ) with the restriction enzymes AgeI and BsrG1 (New England BioLabs) and inserting the PCR-amplified GluA1-SEP from pCI-GluA1-SEP with SgrAI- and BsiWI-cut (New England BioLabs) sticky ends. .. Sequence integrity for the GluA1-SEP was confirmed using primer-walk sequencing (Integrated DNA Technologies).

    Generated:

    Article Title: Plant A20/AN1 protein serves as the important hub to mediate antiviral immunity
    Article Snippet: The overnight yeast culture was diluted to an OD600 of 0.06 and spotted on selection plates (containing histidine- , leucine- , tryptophan- and 5-bromo-4-chloro-3-indolyl-alpha-D-galactopyranoside) for growth assay. .. Fragments of different N-terminus deletions of Pha13 were generated with restriction enzyme digestions of FseI (NEB), ApaI (NEB), Sgr AI (NEB), BtgI (NEB), or PspXI (NEB). .. Fragments of C-terminus deletions were generated by PCR amplification using primer pairs, Nd13Fs412F/ Eco RI-Pha13-R, Nd13Ap361F/ Eco RI-Pha13-R, Nd13Sg247F/ Eco RI-Pha13-R, Nd13Bt136F /Eco RI-Pha13-R, and Nd13Ps88F /Eco RI-Pha13-R ( ).

    Positron Emission Tomography:

    Article Title: Features of the Influence of a DNA Sequence on Its Adjacent Sequence
    Article Snippet: After purification, the concentrations of the digested DNA fragments a , c , g , and t were 6.9, 6.6, 6.9, and 6.6 ng/μL, respectively, which were determined as mentioned above. .. Digestion of the Plasmid pET-42a (+) 1 The digestion reaction: The plasmid pET-42a (+) was digested with SgrAI (NEB, USA) and QuickCut Xba I and Sph I (Takara Bio, China) following the manufacturer’s instruction. .. Briefly, the plasmid pET-42a (+) was digested in 50 μL of digestion reaction mixture containing 1× NEB buffer, 820 ng of plasmid pET-42a (+), 1 μL of each of Sph I and Xba I, and 2 U of SgrAI.

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  • 93
    New England Biolabs sgr ai
    Secondary-site cleavage on ( A ) Age I-linearized and ( B ) Sap I+ Bsa HI-cleaved pSK7 <t>DNA</t> in the presence of oligonucleotide duplex. Lane M, 0.1–10-kb DNA ladder. Lane 1, DNA control (no <t>Sgr</t> AI added). Lanes 2–8: 0, 5, 10, 20, 50, 100, and 200 nM of oligo was added to the 50-μl reactions that contained 5 nM of DNA and 88 nM of Sgr AI and incubated either ( A ) 30 min or ( B ) 4 h at 37°C. ( C ) Nucleotide sequence of the oligonucleotide duplex. Sgr AI canonical termini are shown in bold.
    Sgr Ai, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sgr ai/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sgr ai - by Bioz Stars, 2021-05
    93/100 stars
      Buy from Supplier

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    Secondary-site cleavage on ( A ) Age I-linearized and ( B ) Sap I+ Bsa HI-cleaved pSK7 DNA in the presence of oligonucleotide duplex. Lane M, 0.1–10-kb DNA ladder. Lane 1, DNA control (no Sgr AI added). Lanes 2–8: 0, 5, 10, 20, 50, 100, and 200 nM of oligo was added to the 50-μl reactions that contained 5 nM of DNA and 88 nM of Sgr AI and incubated either ( A ) 30 min or ( B ) 4 h at 37°C. ( C ) Nucleotide sequence of the oligonucleotide duplex. Sgr AI canonical termini are shown in bold.

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Self-generated DNA termini relax the specificity of SgrAI restriction endonuclease

    doi: 10.1073/pnas.022346799

    Figure Lengend Snippet: Secondary-site cleavage on ( A ) Age I-linearized and ( B ) Sap I+ Bsa HI-cleaved pSK7 DNA in the presence of oligonucleotide duplex. Lane M, 0.1–10-kb DNA ladder. Lane 1, DNA control (no Sgr AI added). Lanes 2–8: 0, 5, 10, 20, 50, 100, and 200 nM of oligo was added to the 50-μl reactions that contained 5 nM of DNA and 88 nM of Sgr AI and incubated either ( A ) 30 min or ( B ) 4 h at 37°C. ( C ) Nucleotide sequence of the oligonucleotide duplex. Sgr AI canonical termini are shown in bold.

    Article Snippet: The last unique site, CGCCGGCC, which was not present on the above tested DNA substrates, was determined to be cleaved by Sgr AI by using a derivative of pBR322 that contained two copies of this sequence within the 3.0-kb Pseudomonas alcaligenes genomic DNA insert (R. Vaisvila, New England Biolabs).

    Techniques: Incubation, Sequencing