bmr i  (New England Biolabs)


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  • 94
    Name:
    BmrI
    Description:
    BmrI 100 units
    Catalog Number:
    r0600s
    Price:
    69
    Size:
    100 units
    Category:
    Restriction Enzymes
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    Structured Review

    New England Biolabs bmr i
    BmrI
    BmrI 100 units
    https://www.bioz.com/result/bmr i/product/New England Biolabs
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    bmr i - by Bioz Stars, 2020-07
    94/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Broadly applicable PCR restriction fragment length polymorphism method for genotyping bovine leukemia virus
    Article Snippet: .. RFLP reactions (20 µl ) contained 2.5 µl of purified PCR products, 15 U of Bmr I, Alw I and Hph I (New England Biolabs, Inc.), 20 U of Taq I, Pvu II, Bam H I and Mse I (Takara Bio Inc.), with an appropriate buffer for each restriction endonuclease. .. The reaction mixtures were incubated at 37°C for 2 hr (Bmr I, Hph I, Pvu II andAlw I), 30°C for 2 hr (Bam H I), 60°C for 2 hr (Mse I) and 65°C for 2 hr (Taq I) respectively.

    Article Title: Role of two single nucleotide polymorphisms in secreted frizzled related protein 1 and bladder cancer risk
    Article Snippet: .. The thermal cycling conditions were as follows: initial denaturation for 3 min at 95°C, 35 cycles of denaturation at 95°C for 1 min, annealing at 57°C (rs3242) and 57.8°C (rs921142), respectively, for 1 min, elongation at 72°C for 1 min and final primer extension at 72°C for 10 min. PCR products were incubated for four hours with 5 U Rsa I (New England Biolabs, Frankfurt/Main, Germany) at 37°C (rs3242) and ten hours with 5 U Bmr I (New England Biolabs, Frankfurt/Main, Germany) at 37°C (rs921142) in a total volume of 30 μl to ensure complete digestion. .. Restriction fragments were separated by electrophoresis using 2.5% agarose gels and visualized under ultraviolet light using 0.05% ethidium bromide.

    Concentration Assay:

    Article Title: A standard for near-scarless plasmid construction using reusable DNA parts
    Article Snippet: .. The RE-digestion and ligation solution contained 1 µL of Type IIS RE (MboII [R0148S], HphI [R0158S], BmrI [R0600S] or BciVI [R0596S] NEB), 0.3 µL of 1.25 μM L(G/A)-Boligo, 0.3 µL of 1.25 μM R(G/A)-Boligo, 3 µL of fragment (the concentration of fragment is recommended in section “Barcoding”), and 4.6 µL of Blunt/TA Ligase Master Mix. .. The RE-digestion mixture was incubated at 37 °C for 1 h, and two microliters of the solution was used as template in PCR to amplify barcoded fragment by using corresponding PS-modified Aoligos.

    Incubation:

    Article Title: Role of two single nucleotide polymorphisms in secreted frizzled related protein 1 and bladder cancer risk
    Article Snippet: .. The thermal cycling conditions were as follows: initial denaturation for 3 min at 95°C, 35 cycles of denaturation at 95°C for 1 min, annealing at 57°C (rs3242) and 57.8°C (rs921142), respectively, for 1 min, elongation at 72°C for 1 min and final primer extension at 72°C for 10 min. PCR products were incubated for four hours with 5 U Rsa I (New England Biolabs, Frankfurt/Main, Germany) at 37°C (rs3242) and ten hours with 5 U Bmr I (New England Biolabs, Frankfurt/Main, Germany) at 37°C (rs921142) in a total volume of 30 μl to ensure complete digestion. .. Restriction fragments were separated by electrophoresis using 2.5% agarose gels and visualized under ultraviolet light using 0.05% ethidium bromide.

    Ligation:

    Article Title: A standard for near-scarless plasmid construction using reusable DNA parts
    Article Snippet: .. The RE-digestion and ligation solution contained 1 µL of Type IIS RE (MboII [R0148S], HphI [R0158S], BmrI [R0600S] or BciVI [R0596S] NEB), 0.3 µL of 1.25 μM L(G/A)-Boligo, 0.3 µL of 1.25 μM R(G/A)-Boligo, 3 µL of fragment (the concentration of fragment is recommended in section “Barcoding”), and 4.6 µL of Blunt/TA Ligase Master Mix. .. The RE-digestion mixture was incubated at 37 °C for 1 h, and two microliters of the solution was used as template in PCR to amplify barcoded fragment by using corresponding PS-modified Aoligos.

    Purification:

    Article Title: Broadly applicable PCR restriction fragment length polymorphism method for genotyping bovine leukemia virus
    Article Snippet: .. RFLP reactions (20 µl ) contained 2.5 µl of purified PCR products, 15 U of Bmr I, Alw I and Hph I (New England Biolabs, Inc.), 20 U of Taq I, Pvu II, Bam H I and Mse I (Takara Bio Inc.), with an appropriate buffer for each restriction endonuclease. .. The reaction mixtures were incubated at 37°C for 2 hr (Bmr I, Hph I, Pvu II andAlw I), 30°C for 2 hr (Bam H I), 60°C for 2 hr (Mse I) and 65°C for 2 hr (Taq I) respectively.

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  • About
  • News
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  • Team
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  • Contact
  • Bioz Stars
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  • 94
    New England Biolabs bmr i
    Bmr I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bmr i/product/New England Biolabs
    Average 94 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    bmr i - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

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