bmri  (New England Biolabs)


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  • 96
    Name:
    BmrI
    Description:
    BmrI 100 units
    Catalog Number:
    r0600s
    Price:
    69
    Size:
    100 units
    Category:
    Restriction Enzymes
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    Structured Review

    New England Biolabs bmri
    BmrI
    BmrI 100 units
    https://www.bioz.com/result/bmri/product/New England Biolabs
    Average 96 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    bmri - by Bioz Stars, 2020-09
    96/100 stars

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    Related Articles

    Immunoprecipitation:

    Article Title: TGF- β–induced epigenetic deregulation of SOCS3 facilitates STAT3 signaling to promote fibrosis
    Article Snippet: .. For MeDIP assay, genomic DNA was fractionated by enzymatic digestion with BmrI and EcoP15I (New England Biolabs) and processed for immunoprecipitation using the MeDIP Assay Kit from Active Motif according to the manufacturer’s protocol. .. The primer sequences used for qPCR are given in .

    Ligation:

    Article Title: A standard for near-scarless plasmid construction using reusable DNA parts
    Article Snippet: .. The RE-digestion and ligation solution contained 1 µL of Type IIS RE (MboII [R0148S], HphI [R0158S], BmrI [R0600S] or BciVI [R0596S] NEB), 0.3 µL of 1.25 μM L(G/A)-Boligo, 0.3 µL of 1.25 μM R(G/A)-Boligo, 3 µL of fragment (the concentration of fragment is recommended in section “Barcoding”), and 4.6 µL of Blunt/TA Ligase Master Mix. .. The RE-digestion mixture was incubated at 37 °C for 1 h, and two microliters of the solution was used as template in PCR to amplify barcoded fragment by using corresponding PS-modified Aoligos.

    Purification:

    Article Title: Broadly applicable PCR restriction fragment length polymorphism method for genotyping bovine leukemia virus
    Article Snippet: .. RFLP reactions (20 µl ) contained 2.5 µl of purified PCR products, 15 U of Bmr I, Alw I and Hph I (New England Biolabs, Inc.), 20 U of Taq I, Pvu II, Bam H I and Mse I (Takara Bio Inc.), with an appropriate buffer for each restriction endonuclease. .. The reaction mixtures were incubated at 37°C for 2 hr (Bmr I, Hph I, Pvu II andAlw I), 30°C for 2 hr (Bam H I), 60°C for 2 hr (Mse I) and 65°C for 2 hr (Taq I) respectively.

    Concentration Assay:

    Article Title: A standard for near-scarless plasmid construction using reusable DNA parts
    Article Snippet: .. The RE-digestion and ligation solution contained 1 µL of Type IIS RE (MboII [R0148S], HphI [R0158S], BmrI [R0600S] or BciVI [R0596S] NEB), 0.3 µL of 1.25 μM L(G/A)-Boligo, 0.3 µL of 1.25 μM R(G/A)-Boligo, 3 µL of fragment (the concentration of fragment is recommended in section “Barcoding”), and 4.6 µL of Blunt/TA Ligase Master Mix. .. The RE-digestion mixture was incubated at 37 °C for 1 h, and two microliters of the solution was used as template in PCR to amplify barcoded fragment by using corresponding PS-modified Aoligos.

    Incubation:

    Article Title: Role of two single nucleotide polymorphisms in secreted frizzled related protein 1 and bladder cancer risk
    Article Snippet: .. The thermal cycling conditions were as follows: initial denaturation for 3 min at 95°C, 35 cycles of denaturation at 95°C for 1 min, annealing at 57°C (rs3242) and 57.8°C (rs921142), respectively, for 1 min, elongation at 72°C for 1 min and final primer extension at 72°C for 10 min. PCR products were incubated for four hours with 5 U Rsa I (New England Biolabs, Frankfurt/Main, Germany) at 37°C (rs3242) and ten hours with 5 U Bmr I (New England Biolabs, Frankfurt/Main, Germany) at 37°C (rs921142) in a total volume of 30 μl to ensure complete digestion. .. Restriction fragments were separated by electrophoresis using 2.5% agarose gels and visualized under ultraviolet light using 0.05% ethidium bromide.

    Polymerase Chain Reaction:

    Article Title: Broadly applicable PCR restriction fragment length polymorphism method for genotyping bovine leukemia virus
    Article Snippet: .. RFLP reactions (20 µl ) contained 2.5 µl of purified PCR products, 15 U of Bmr I, Alw I and Hph I (New England Biolabs, Inc.), 20 U of Taq I, Pvu II, Bam H I and Mse I (Takara Bio Inc.), with an appropriate buffer for each restriction endonuclease. .. The reaction mixtures were incubated at 37°C for 2 hr (Bmr I, Hph I, Pvu II andAlw I), 30°C for 2 hr (Bam H I), 60°C for 2 hr (Mse I) and 65°C for 2 hr (Taq I) respectively.

    Article Title: Role of two single nucleotide polymorphisms in secreted frizzled related protein 1 and bladder cancer risk
    Article Snippet: .. The thermal cycling conditions were as follows: initial denaturation for 3 min at 95°C, 35 cycles of denaturation at 95°C for 1 min, annealing at 57°C (rs3242) and 57.8°C (rs921142), respectively, for 1 min, elongation at 72°C for 1 min and final primer extension at 72°C for 10 min. PCR products were incubated for four hours with 5 U Rsa I (New England Biolabs, Frankfurt/Main, Germany) at 37°C (rs3242) and ten hours with 5 U Bmr I (New England Biolabs, Frankfurt/Main, Germany) at 37°C (rs921142) in a total volume of 30 μl to ensure complete digestion. .. Restriction fragments were separated by electrophoresis using 2.5% agarose gels and visualized under ultraviolet light using 0.05% ethidium bromide.

    Methylated DNA Immunoprecipitation:

    Article Title: TGF- β–induced epigenetic deregulation of SOCS3 facilitates STAT3 signaling to promote fibrosis
    Article Snippet: .. For MeDIP assay, genomic DNA was fractionated by enzymatic digestion with BmrI and EcoP15I (New England Biolabs) and processed for immunoprecipitation using the MeDIP Assay Kit from Active Motif according to the manufacturer’s protocol. .. The primer sequences used for qPCR are given in .

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  • 96
    New England Biolabs bmri
    Bmri, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bmri/product/New England Biolabs
    Average 96 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    bmri - by Bioz Stars, 2020-09
    96/100 stars
      Buy from Supplier

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