smli  (New England Biolabs)


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  • 99
    Name:
    SmlI
    Description:
    SmlI 2 500 units
    Catalog Number:
    r0597l
    Price:
    282
    Size:
    2 500 units
    Category:
    Restriction Enzymes
    Buy from Supplier


    Structured Review

    New England Biolabs smli
    SmlI
    SmlI 2 500 units
    https://www.bioz.com/result/smli/product/New England Biolabs
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    smli - by Bioz Stars, 2020-03
    99/100 stars

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    Related Articles

    Multiplex Assay:

    Article Title: Population-Based Molecular Epidemiology of Leprosy in Cebu, Philippines
    Article Snippet: Single or multiplex PCRs were set up using 1 to 2 μl of the DNA extract. .. Restriction digest assays were developed using SmlI, CviKI-1, and BstUI for SNP loci 1, 2, and 3, respectively (New England Biolabs, MA).

    Amplification:

    Article Title: Neuroprotective Effect of Over Expression of Thioredoxin on Photoreceptor Degeneration in Tubby Mice
    Article Snippet: Genomic DNA from mouse tail was amplified by PCR using the following two sets of primers: tubby (forward primer, 5′-ccgtgtcacacaggcttct-3′ and reverse primer, 5′-ctgggcaccatgcgtaca-3′) and Tg-Trx (Forward, 5’-AAGCAGATCGAGAGCAAGACT-3’, Rev, 5’-TCCTTATTGGCTCCAGAAAAT-3’. .. After 94°C for15 min, 35 cycles were run (94°C 30 s, 55°C 30 s, 72°C 30 sec terminating with 72°C 2 min, 10°C 10 min) and the PCR products of tubby were digested with the SmlI restriction enzyme (New England Biolabs, Ipswich, MA) and analyzed by 5% MetaPhor agarose gel (Lonza, Rockland, ME) electrophoresis.

    Article Title: Zebrafish bmp4 functions during late gastrulation to specify ventroposterior cell fates
    Article Snippet: 10 ug of the genomic DNA was digested with BstXI, PsiI, HpyCHIV, NheI, SmlI, SfcI, MmeI, BspMI, or BssSI (NEB), run on a 1% agarose gel, and then transferred to a charged nylon membrane (Hybond-N+, Amersham Biosciences). .. Probes were amplified from genomic DNA (5′ probe: 5′-CCGCTGATAAATAAGAGACTAAGC-3′ and 5′-GCTTTAAGCATAATCTGAAATCACTC-3′, 667bp; 3′probe: 5′-ACACCGTGCGCATCTCTT-3′ and 5′-GGCTGCTAATATGATTTCCCTGT-3′, 579bp) and radioactively labeled with the Rediprime II Random Prime Labelling System (Amersham Biosciences).

    Agarose Gel Electrophoresis:

    Article Title: Variable phenotypic expression and onset in MYH14 distal HMN phenotype in a large, multigenerational North American family
    Article Snippet: .. PCR products were digested with SmlI (Catalogue Number: R0597S, NEB) for 2 hours at 37°C and run on a 2% agarose gel. ..

    Article Title: Neuroprotective Effect of Over Expression of Thioredoxin on Photoreceptor Degeneration in Tubby Mice
    Article Snippet: .. After 94°C for15 min, 35 cycles were run (94°C 30 s, 55°C 30 s, 72°C 30 sec terminating with 72°C 2 min, 10°C 10 min) and the PCR products of tubby were digested with the SmlI restriction enzyme (New England Biolabs, Ipswich, MA) and analyzed by 5% MetaPhor agarose gel (Lonza, Rockland, ME) electrophoresis. .. The 101 bp wild-type (+/+) product is undigested, the tubby mutant product is digested to 52 bp and 49 bp products, and tub/+ shows all three products of 101 bp, 52 bp, and 49 bp.

    Article Title: Zebrafish bmp4 functions during late gastrulation to specify ventroposterior cell fates
    Article Snippet: .. 10 ug of the genomic DNA was digested with BstXI, PsiI, HpyCHIV, NheI, SmlI, SfcI, MmeI, BspMI, or BssSI (NEB), run on a 1% agarose gel, and then transferred to a charged nylon membrane (Hybond-N+, Amersham Biosciences). .. Probes were amplified from genomic DNA (5′ probe: 5′-CCGCTGATAAATAAGAGACTAAGC-3′ and 5′-GCTTTAAGCATAATCTGAAATCACTC-3′, 667bp; 3′probe: 5′-ACACCGTGCGCATCTCTT-3′ and 5′-GGCTGCTAATATGATTTCCCTGT-3′, 579bp) and radioactively labeled with the Rediprime II Random Prime Labelling System (Amersham Biosciences).

    Article Title: Population-Based Molecular Epidemiology of Leprosy in Cebu, Philippines
    Article Snippet: The DNA products were resolved by agarose gel electrophoresis and detected by ethidium bromide staining. .. Restriction digest assays were developed using SmlI, CviKI-1, and BstUI for SNP loci 1, 2, and 3, respectively (New England Biolabs, MA).

    Mutagenesis:

    Article Title: Variable phenotypic expression and onset in MYH14 distal HMN phenotype in a large, multigenerational North American family
    Article Snippet: The MYC14 mutation creates an SmlI site. .. PCR products were digested with SmlI (Catalogue Number: R0597S, NEB) for 2 hours at 37°C and run on a 2% agarose gel.

    Article Title: Neuroprotective Effect of Over Expression of Thioredoxin on Photoreceptor Degeneration in Tubby Mice
    Article Snippet: After 94°C for15 min, 35 cycles were run (94°C 30 s, 55°C 30 s, 72°C 30 sec terminating with 72°C 2 min, 10°C 10 min) and the PCR products of tubby were digested with the SmlI restriction enzyme (New England Biolabs, Ipswich, MA) and analyzed by 5% MetaPhor agarose gel (Lonza, Rockland, ME) electrophoresis. .. The 101 bp wild-type (+/+) product is undigested, the tubby mutant product is digested to 52 bp and 49 bp products, and tub/+ shows all three products of 101 bp, 52 bp, and 49 bp.

    Isolation:

    Article Title: Zebrafish bmp4 functions during late gastrulation to specify ventroposterior cell fates
    Article Snippet: Genomic DNA was isolated from pools of 40 st37 / st37 , st37 /+, and +/+ embryos at 5dpf with a Qiagen DNeasy kit. .. 10 ug of the genomic DNA was digested with BstXI, PsiI, HpyCHIV, NheI, SmlI, SfcI, MmeI, BspMI, or BssSI (NEB), run on a 1% agarose gel, and then transferred to a charged nylon membrane (Hybond-N+, Amersham Biosciences).

    Size-exclusion Chromatography:

    Article Title: Neuroprotective Effect of Over Expression of Thioredoxin on Photoreceptor Degeneration in Tubby Mice
    Article Snippet: .. After 94°C for15 min, 35 cycles were run (94°C 30 s, 55°C 30 s, 72°C 30 sec terminating with 72°C 2 min, 10°C 10 min) and the PCR products of tubby were digested with the SmlI restriction enzyme (New England Biolabs, Ipswich, MA) and analyzed by 5% MetaPhor agarose gel (Lonza, Rockland, ME) electrophoresis. .. The 101 bp wild-type (+/+) product is undigested, the tubby mutant product is digested to 52 bp and 49 bp products, and tub/+ shows all three products of 101 bp, 52 bp, and 49 bp.

    Labeling:

    Article Title: Zebrafish bmp4 functions during late gastrulation to specify ventroposterior cell fates
    Article Snippet: 10 ug of the genomic DNA was digested with BstXI, PsiI, HpyCHIV, NheI, SmlI, SfcI, MmeI, BspMI, or BssSI (NEB), run on a 1% agarose gel, and then transferred to a charged nylon membrane (Hybond-N+, Amersham Biosciences). .. Probes were amplified from genomic DNA (5′ probe: 5′-CCGCTGATAAATAAGAGACTAAGC-3′ and 5′-GCTTTAAGCATAATCTGAAATCACTC-3′, 667bp; 3′probe: 5′-ACACCGTGCGCATCTCTT-3′ and 5′-GGCTGCTAATATGATTTCCCTGT-3′, 579bp) and radioactively labeled with the Rediprime II Random Prime Labelling System (Amersham Biosciences).

    Mouse Assay:

    Article Title: Neuroprotective Effect of Over Expression of Thioredoxin on Photoreceptor Degeneration in Tubby Mice
    Article Snippet: Prior to conducting experiments routine polymerase chain reaction (PCR) was used to identify the genotypes of various mice as described previously ( ). .. After 94°C for15 min, 35 cycles were run (94°C 30 s, 55°C 30 s, 72°C 30 sec terminating with 72°C 2 min, 10°C 10 min) and the PCR products of tubby were digested with the SmlI restriction enzyme (New England Biolabs, Ipswich, MA) and analyzed by 5% MetaPhor agarose gel (Lonza, Rockland, ME) electrophoresis.

    Electrophoresis:

    Article Title: Neuroprotective Effect of Over Expression of Thioredoxin on Photoreceptor Degeneration in Tubby Mice
    Article Snippet: .. After 94°C for15 min, 35 cycles were run (94°C 30 s, 55°C 30 s, 72°C 30 sec terminating with 72°C 2 min, 10°C 10 min) and the PCR products of tubby were digested with the SmlI restriction enzyme (New England Biolabs, Ipswich, MA) and analyzed by 5% MetaPhor agarose gel (Lonza, Rockland, ME) electrophoresis. .. The 101 bp wild-type (+/+) product is undigested, the tubby mutant product is digested to 52 bp and 49 bp products, and tub/+ shows all three products of 101 bp, 52 bp, and 49 bp.

    Article Title: Population-Based Molecular Epidemiology of Leprosy in Cebu, Philippines
    Article Snippet: Restriction digest assays were developed using SmlI, CviKI-1, and BstUI for SNP loci 1, 2, and 3, respectively (New England Biolabs, MA). .. The SmlI, BstUI, and CviKI-1 reactions were performed at 55°C, 60°C, and 37°C, respectively, for 1 h. The SmlI- and BstUI-uncut and -cut DNAs were subjected to electrophoresis on 3% agarose gels.

    Polymerase Chain Reaction:

    Article Title: Variable phenotypic expression and onset in MYH14 distal HMN phenotype in a large, multigenerational North American family
    Article Snippet: .. PCR products were digested with SmlI (Catalogue Number: R0597S, NEB) for 2 hours at 37°C and run on a 2% agarose gel. ..

    Article Title: Neuroprotective Effect of Over Expression of Thioredoxin on Photoreceptor Degeneration in Tubby Mice
    Article Snippet: .. After 94°C for15 min, 35 cycles were run (94°C 30 s, 55°C 30 s, 72°C 30 sec terminating with 72°C 2 min, 10°C 10 min) and the PCR products of tubby were digested with the SmlI restriction enzyme (New England Biolabs, Ipswich, MA) and analyzed by 5% MetaPhor agarose gel (Lonza, Rockland, ME) electrophoresis. .. The 101 bp wild-type (+/+) product is undigested, the tubby mutant product is digested to 52 bp and 49 bp products, and tub/+ shows all three products of 101 bp, 52 bp, and 49 bp.

    Article Title: Population-Based Molecular Epidemiology of Leprosy in Cebu, Philippines
    Article Snippet: Paragraph title: Development of a PCR-RFLP method for SNP subtyping of M. leprae . ... Restriction digest assays were developed using SmlI, CviKI-1, and BstUI for SNP loci 1, 2, and 3, respectively (New England Biolabs, MA).

    Generated:

    Article Title: Neuroprotective Effect of Over Expression of Thioredoxin on Photoreceptor Degeneration in Tubby Mice
    Article Snippet: The tubby Tg-Trx mouse was generated by cross-breeding these two lines ( tub/Trx ). .. After 94°C for15 min, 35 cycles were run (94°C 30 s, 55°C 30 s, 72°C 30 sec terminating with 72°C 2 min, 10°C 10 min) and the PCR products of tubby were digested with the SmlI restriction enzyme (New England Biolabs, Ipswich, MA) and analyzed by 5% MetaPhor agarose gel (Lonza, Rockland, ME) electrophoresis.

    Acrylamide Gel Assay:

    Article Title: Population-Based Molecular Epidemiology of Leprosy in Cebu, Philippines
    Article Snippet: Restriction digest assays were developed using SmlI, CviKI-1, and BstUI for SNP loci 1, 2, and 3, respectively (New England Biolabs, MA). .. The uncut and CviKI-1-digested SNP locus 2 products were resolved on a 12% acrylamide gel.

    Staining:

    Article Title: Population-Based Molecular Epidemiology of Leprosy in Cebu, Philippines
    Article Snippet: The DNA products were resolved by agarose gel electrophoresis and detected by ethidium bromide staining. .. Restriction digest assays were developed using SmlI, CviKI-1, and BstUI for SNP loci 1, 2, and 3, respectively (New England Biolabs, MA).

    Variant Assay:

    Article Title: Variable phenotypic expression and onset in MYH14 distal HMN phenotype in a large, multigenerational North American family
    Article Snippet: Paragraph title: Variant validation ... PCR products were digested with SmlI (Catalogue Number: R0597S, NEB) for 2 hours at 37°C and run on a 2% agarose gel.

    Hybridization:

    Article Title: Zebrafish bmp4 functions during late gastrulation to specify ventroposterior cell fates
    Article Snippet: 10 ug of the genomic DNA was digested with BstXI, PsiI, HpyCHIV, NheI, SmlI, SfcI, MmeI, BspMI, or BssSI (NEB), run on a 1% agarose gel, and then transferred to a charged nylon membrane (Hybond-N+, Amersham Biosciences). .. Hybridization of the probes to the nylon membrane was performed with Rapid-hyb buffer (Amersham Biosciences) following the supplied protocol.

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  • 80
    New England Biolabs smli restriction enzyme
    Smli Restriction Enzyme, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 80/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/smli restriction enzyme/product/New England Biolabs
    Average 80 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    smli restriction enzyme - by Bioz Stars, 2020-03
    80/100 stars
      Buy from Supplier

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