bcivi  (New England Biolabs)


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  • 94
    Name:
    BciVI
    Description:
    BciVI 1 000 units
    Catalog Number:
    R0596L
    Price:
    269
    Category:
    Restriction Enzymes
    Size:
    1 000 units
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    Structured Review

    New England Biolabs bcivi
    BciVI
    BciVI 1 000 units
    https://www.bioz.com/result/bcivi/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bcivi - by Bioz Stars, 2021-06
    94/100 stars

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    Related Articles

    Ligation:

    Article Title: pGP-B2E, a Recombinant Compatible TA/TB-Ligation Vector for Rapid and Inexpensive Gene Cloning.
    Article Snippet: DNA cloning is the basic step for different fields of life science, and many efforts have been made to simplify this procedure. .. DNA cloning is the basic step for different fields of life science, and many efforts have been made to simplify this procedure. .. DNA cloning is the basic step for different fields of life science, and many efforts have been made to simplify this procedure.

    Plasmid Preparation:

    Article Title: pGP-B2E, a Recombinant Compatible TA/TB-Ligation Vector for Rapid and Inexpensive Gene Cloning.
    Article Snippet: DNA cloning is the basic step for different fields of life science, and many efforts have been made to simplify this procedure. .. DNA cloning is the basic step for different fields of life science, and many efforts have been made to simplify this procedure. .. DNA cloning is the basic step for different fields of life science, and many efforts have been made to simplify this procedure.

    Purification:

    Article Title: Adaptation in protein fitness landscapes is facilitated by indirect paths
    Article Snippet: The product (constant region) was purified by PureLink PCR Purification Kit (Life Technologies) according to manufacturer’s instructions. .. Both the purified constant region and cassette I were digested with BciVI (New England Biolabs) and purified by PureLink PCR Purification Kit (Life Technologies) according to manufacturer’s instructions. .. Ligation between the constant region and cassette I (molar ratio of 1:1) was performed using T4 DNA ligase (New England Biolabs).

    Polymerase Chain Reaction:

    Article Title: Adaptation in protein fitness landscapes is facilitated by indirect paths
    Article Snippet: The product (constant region) was purified by PureLink PCR Purification Kit (Life Technologies) according to manufacturer’s instructions. .. Both the purified constant region and cassette I were digested with BciVI (New England Biolabs) and purified by PureLink PCR Purification Kit (Life Technologies) according to manufacturer’s instructions. .. Ligation between the constant region and cassette I (molar ratio of 1:1) was performed using T4 DNA ligase (New England Biolabs).

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  • 94
    New England Biolabs bciv i
    Analysis of replication through CAG-70 and CAG-130 repeats by two-dimensional (2D) agarose gel electrophoresis in WT, tof1 Δ and mrc1 Δ strains. ( A ) Schematic of the pYES2 constructs is shown with its mass and genetic map. The relative positions of its most relevant features are indicated inside: the 2 μm origin, the ColE1 unidirectional origin (ColE1 Ori), the ampicillin-resistance gene (Amp R ), URA3 , the GAL1 promoter (Gal1 prom) and 70 or 130 CAG repeats. Outside, the relative positions of sites recognized by the restriction endonucleases Nde I and <t>BciV</t> I are indicated. To the right, is shown the corresponding linear map of the pYES2 plasmid restriction fragment with the sizes and the diagrammatic interpretation if replication initiates bi-directionally at the 2 μm origin and proceeds unconstrained. ( B ) Representative 2D gels of replication through CAG-70 and CAG-130 repeats in WT, tof1 Δ, and mrc1 Δ strains. <t>DNA</t> was isolated, digested with Nde I and BciV I and analyzed by 2D gel. Red arrow points to the location of the CAG repeats. To the right of each 2D gel are shown the densitometric profiles corresponding to the Y-arc region where the (CAG)n repeats are located; peaks on densitograms correspond to bulges on the Y-arcs. A representative gel and its corresponding profile is shown; three experiments were analyzed for each strain. ( C ) Quantification of replication fork slowing in pYES2 CAG-130 in WT, tof1 Δ and mrc1 Δ strains. The ratio of radioactivity in the peak area to that corresponding area of a smooth replication arc reflects the extent of replication slowing. Three different experiments were performed for each strain. Percentage of replication fork slowing is 3.3%, 4.6% and 5.8% for WT, 13.2%, 17.7% and 20.4% for tof1 Δ, and 8.3%, 16.4% and 27.7% for mrc1 Δ. Error bars indicate standard error of the mean. The star indicates a significant difference between wild-type and mutants. P = 0.0483 ( tof1 Δ versus WT), P = 0.0378 ( mrc1 Δ versus WT).
    Bciv I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bciv i/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bciv i - by Bioz Stars, 2021-06
    94/100 stars
      Buy from Supplier

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    Analysis of replication through CAG-70 and CAG-130 repeats by two-dimensional (2D) agarose gel electrophoresis in WT, tof1 Δ and mrc1 Δ strains. ( A ) Schematic of the pYES2 constructs is shown with its mass and genetic map. The relative positions of its most relevant features are indicated inside: the 2 μm origin, the ColE1 unidirectional origin (ColE1 Ori), the ampicillin-resistance gene (Amp R ), URA3 , the GAL1 promoter (Gal1 prom) and 70 or 130 CAG repeats. Outside, the relative positions of sites recognized by the restriction endonucleases Nde I and BciV I are indicated. To the right, is shown the corresponding linear map of the pYES2 plasmid restriction fragment with the sizes and the diagrammatic interpretation if replication initiates bi-directionally at the 2 μm origin and proceeds unconstrained. ( B ) Representative 2D gels of replication through CAG-70 and CAG-130 repeats in WT, tof1 Δ, and mrc1 Δ strains. DNA was isolated, digested with Nde I and BciV I and analyzed by 2D gel. Red arrow points to the location of the CAG repeats. To the right of each 2D gel are shown the densitometric profiles corresponding to the Y-arc region where the (CAG)n repeats are located; peaks on densitograms correspond to bulges on the Y-arcs. A representative gel and its corresponding profile is shown; three experiments were analyzed for each strain. ( C ) Quantification of replication fork slowing in pYES2 CAG-130 in WT, tof1 Δ and mrc1 Δ strains. The ratio of radioactivity in the peak area to that corresponding area of a smooth replication arc reflects the extent of replication slowing. Three different experiments were performed for each strain. Percentage of replication fork slowing is 3.3%, 4.6% and 5.8% for WT, 13.2%, 17.7% and 20.4% for tof1 Δ, and 8.3%, 16.4% and 27.7% for mrc1 Δ. Error bars indicate standard error of the mean. The star indicates a significant difference between wild-type and mutants. P = 0.0483 ( tof1 Δ versus WT), P = 0.0378 ( mrc1 Δ versus WT).

    Journal: Nucleic Acids Research

    Article Title: Mrc1 and Tof1 prevent fragility and instability at long CAG repeats by their fork stabilizing function

    doi: 10.1093/nar/gky1195

    Figure Lengend Snippet: Analysis of replication through CAG-70 and CAG-130 repeats by two-dimensional (2D) agarose gel electrophoresis in WT, tof1 Δ and mrc1 Δ strains. ( A ) Schematic of the pYES2 constructs is shown with its mass and genetic map. The relative positions of its most relevant features are indicated inside: the 2 μm origin, the ColE1 unidirectional origin (ColE1 Ori), the ampicillin-resistance gene (Amp R ), URA3 , the GAL1 promoter (Gal1 prom) and 70 or 130 CAG repeats. Outside, the relative positions of sites recognized by the restriction endonucleases Nde I and BciV I are indicated. To the right, is shown the corresponding linear map of the pYES2 plasmid restriction fragment with the sizes and the diagrammatic interpretation if replication initiates bi-directionally at the 2 μm origin and proceeds unconstrained. ( B ) Representative 2D gels of replication through CAG-70 and CAG-130 repeats in WT, tof1 Δ, and mrc1 Δ strains. DNA was isolated, digested with Nde I and BciV I and analyzed by 2D gel. Red arrow points to the location of the CAG repeats. To the right of each 2D gel are shown the densitometric profiles corresponding to the Y-arc region where the (CAG)n repeats are located; peaks on densitograms correspond to bulges on the Y-arcs. A representative gel and its corresponding profile is shown; three experiments were analyzed for each strain. ( C ) Quantification of replication fork slowing in pYES2 CAG-130 in WT, tof1 Δ and mrc1 Δ strains. The ratio of radioactivity in the peak area to that corresponding area of a smooth replication arc reflects the extent of replication slowing. Three different experiments were performed for each strain. Percentage of replication fork slowing is 3.3%, 4.6% and 5.8% for WT, 13.2%, 17.7% and 20.4% for tof1 Δ, and 8.3%, 16.4% and 27.7% for mrc1 Δ. Error bars indicate standard error of the mean. The star indicates a significant difference between wild-type and mutants. P = 0.0483 ( tof1 Δ versus WT), P = 0.0378 ( mrc1 Δ versus WT).

    Article Snippet: DNA was digested by Nde I, BciV I and Psi I (New England Biolabs) for 7 h at 37°C.

    Techniques: Agarose Gel Electrophoresis, Construct, Plasmid Preparation, Isolation, Two-Dimensional Gel Electrophoresis, Radioactivity

    Analysis of replication through CAG-70 and CAG-130 repeats by two-dimensional (2D) agarose gel electrophoresis in WT, tof1 Δ and mrc1 Δ strains. ( A ) Schematic of the pYES2 constructs is shown with its mass and genetic map. The relative positions of its most relevant features are indicated inside: the 2 μm origin, the ColE1 unidirectional origin (ColE1 Ori), the ampicillin-resistance gene (Amp R ), URA3 , the GAL1 promoter (Gal1 prom) and 70 or 130 CAG repeats. Outside, the relative positions of sites recognized by the restriction endonucleases Nde I and BciV I are indicated. To the right, is shown the corresponding linear map of the pYES2 plasmid restriction fragment with the sizes and the diagrammatic interpretation if replication initiates bi-directionally at the 2 μm origin and proceeds unconstrained. ( B ) Representative 2D gels of replication through CAG-70 and CAG-130 repeats in WT, tof1 Δ, and mrc1 Δ strains. DNA was isolated, digested with Nde I and BciV I and analyzed by 2D gel. Red arrow points to the location of the CAG repeats. To the right of each 2D gel are shown the densitometric profiles corresponding to the Y-arc region where the (CAG)n repeats are located; peaks on densitograms correspond to bulges on the Y-arcs. A representative gel and its corresponding profile is shown; three experiments were analyzed for each strain. ( C ) Quantification of replication fork slowing in pYES2 CAG-130 in WT, tof1 Δ and mrc1 Δ strains. The ratio of radioactivity in the peak area to that corresponding area of a smooth replication arc reflects the extent of replication slowing. Three different experiments were performed for each strain. Percentage of replication fork slowing is 3.3%, 4.6% and 5.8% for WT, 13.2%, 17.7% and 20.4% for tof1 Δ, and 8.3%, 16.4% and 27.7% for mrc1 Δ. Error bars indicate standard error of the mean. The star indicates a significant difference between wild-type and mutants. P = 0.0483 ( tof1 Δ versus WT), P = 0.0378 ( mrc1 Δ versus WT).

    Journal: Nucleic Acids Research

    Article Title: Mrc1 and Tof1 prevent fragility and instability at long CAG repeats by their fork stabilizing function

    doi: 10.1093/nar/gky1195

    Figure Lengend Snippet: Analysis of replication through CAG-70 and CAG-130 repeats by two-dimensional (2D) agarose gel electrophoresis in WT, tof1 Δ and mrc1 Δ strains. ( A ) Schematic of the pYES2 constructs is shown with its mass and genetic map. The relative positions of its most relevant features are indicated inside: the 2 μm origin, the ColE1 unidirectional origin (ColE1 Ori), the ampicillin-resistance gene (Amp R ), URA3 , the GAL1 promoter (Gal1 prom) and 70 or 130 CAG repeats. Outside, the relative positions of sites recognized by the restriction endonucleases Nde I and BciV I are indicated. To the right, is shown the corresponding linear map of the pYES2 plasmid restriction fragment with the sizes and the diagrammatic interpretation if replication initiates bi-directionally at the 2 μm origin and proceeds unconstrained. ( B ) Representative 2D gels of replication through CAG-70 and CAG-130 repeats in WT, tof1 Δ, and mrc1 Δ strains. DNA was isolated, digested with Nde I and BciV I and analyzed by 2D gel. Red arrow points to the location of the CAG repeats. To the right of each 2D gel are shown the densitometric profiles corresponding to the Y-arc region where the (CAG)n repeats are located; peaks on densitograms correspond to bulges on the Y-arcs. A representative gel and its corresponding profile is shown; three experiments were analyzed for each strain. ( C ) Quantification of replication fork slowing in pYES2 CAG-130 in WT, tof1 Δ and mrc1 Δ strains. The ratio of radioactivity in the peak area to that corresponding area of a smooth replication arc reflects the extent of replication slowing. Three different experiments were performed for each strain. Percentage of replication fork slowing is 3.3%, 4.6% and 5.8% for WT, 13.2%, 17.7% and 20.4% for tof1 Δ, and 8.3%, 16.4% and 27.7% for mrc1 Δ. Error bars indicate standard error of the mean. The star indicates a significant difference between wild-type and mutants. P = 0.0483 ( tof1 Δ versus WT), P = 0.0378 ( mrc1 Δ versus WT).

    Article Snippet: DNA was digested by Nde I, BciV I and Psi I (New England Biolabs) for 7 h at 37°C.

    Techniques: Agarose Gel Electrophoresis, Construct, Plasmid Preparation, Isolation, Two-Dimensional Gel Electrophoresis, Radioactivity