pflfi  (New England Biolabs)


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Bioz Manufacturer Symbol New England Biolabs manufactures this product  
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  • 93
    Name:
    PflFI
    Description:

    Catalog Number:
    R0595
    Price:
    67
    Category:
    Restriction Enzymes
    Applications:
    DNA Manipulation
    Size:
    2000 units
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    Structured Review

    New England Biolabs pflfi
    PflFI

    https://www.bioz.com/result/pflfi/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pflfi - by Bioz Stars, 2021-08
    93/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Generation of Monoclonal Antibodies against Immunoglobulin Proteins of the Domestic Ferret (Mustela putorius furo)
    Article Snippet: .. A poly-A tail was added to products following completion of the second round of PCR by addition of 5 units recombinant Taq polymerase (ThermoFisher, Cat #EP0402) directly into the reaction and incubation at 72°C for 10 min. Products from Igκ PCR were further purified with QIAquick PCR spin columns before digestion with restriction enzymes PflFI (NEB, Cat #R0595S) or PflmI (NEB, Cat #R0509S) to disrupt the rearranged Vκ21-12 gene from the SP2/0 fusion partner. ..

    Article Title: Somatic hypermutation as a generator of antinuclear antibodies in a murine model of systemic autoimmunity
    Article Snippet: .. Light chain PCR products were purified with QIAquick and digested with restriction enzymes PflFI or PflmI (New England Biolabs, Inc.) to disrupt the rearranged Vk 21-12 gene transcribed by the SP2/0 fusion partner (only three other kappa genes have both restriction sites). ..

    Recombinant:

    Article Title: Generation of Monoclonal Antibodies against Immunoglobulin Proteins of the Domestic Ferret (Mustela putorius furo)
    Article Snippet: .. A poly-A tail was added to products following completion of the second round of PCR by addition of 5 units recombinant Taq polymerase (ThermoFisher, Cat #EP0402) directly into the reaction and incubation at 72°C for 10 min. Products from Igκ PCR were further purified with QIAquick PCR spin columns before digestion with restriction enzymes PflFI (NEB, Cat #R0595S) or PflmI (NEB, Cat #R0509S) to disrupt the rearranged Vκ21-12 gene from the SP2/0 fusion partner. ..

    Incubation:

    Article Title: Generation of Monoclonal Antibodies against Immunoglobulin Proteins of the Domestic Ferret (Mustela putorius furo)
    Article Snippet: .. A poly-A tail was added to products following completion of the second round of PCR by addition of 5 units recombinant Taq polymerase (ThermoFisher, Cat #EP0402) directly into the reaction and incubation at 72°C for 10 min. Products from Igκ PCR were further purified with QIAquick PCR spin columns before digestion with restriction enzymes PflFI (NEB, Cat #R0595S) or PflmI (NEB, Cat #R0509S) to disrupt the rearranged Vκ21-12 gene from the SP2/0 fusion partner. ..

    Purification:

    Article Title: Generation of Monoclonal Antibodies against Immunoglobulin Proteins of the Domestic Ferret (Mustela putorius furo)
    Article Snippet: .. A poly-A tail was added to products following completion of the second round of PCR by addition of 5 units recombinant Taq polymerase (ThermoFisher, Cat #EP0402) directly into the reaction and incubation at 72°C for 10 min. Products from Igκ PCR were further purified with QIAquick PCR spin columns before digestion with restriction enzymes PflFI (NEB, Cat #R0595S) or PflmI (NEB, Cat #R0509S) to disrupt the rearranged Vκ21-12 gene from the SP2/0 fusion partner. ..

    Article Title: Somatic hypermutation as a generator of antinuclear antibodies in a murine model of systemic autoimmunity
    Article Snippet: .. Light chain PCR products were purified with QIAquick and digested with restriction enzymes PflFI or PflmI (New England Biolabs, Inc.) to disrupt the rearranged Vk 21-12 gene transcribed by the SP2/0 fusion partner (only three other kappa genes have both restriction sites). ..

    Mutagenesis:

    Article Title: Mitochondrial Haplotype Alters Mammary Cancer Tumorigenicity and Metastasis in an Oncogenic Driver–Dependent Manner
    Article Snippet: .. The mutation at 9348, present in BALB/cJ mtDNA, results in the incorporation of a restriction digest site for Pflf1 (New England Biolabs: R0595S). ..

    Article Title: Mitochondrial haplotype of the host stromal microenvironment alters metastasis in a non-cell autonomous manner
    Article Snippet: .. The mutation at position 9348 in BALB/cJ mtDNA results in the incorporation of a restriction digest site for Pflf1 (New England Biolabs: R0595S). ..

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  • 93
    New England Biolabs pflfi
    Pflfi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pflfi/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pflfi - by Bioz Stars, 2021-08
    93/100 stars
      Buy from Supplier

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