pshai  (New England Biolabs)


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    Name:
    PshAI
    Description:
    PshAI 5 000 units
    Catalog Number:
    R0593L
    Price:
    282
    Category:
    Restriction Enzymes
    Size:
    5 000 units
    Buy from Supplier


    Structured Review

    New England Biolabs pshai
    PshAI
    PshAI 5 000 units
    https://www.bioz.com/result/pshai/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pshai - by Bioz Stars, 2021-06
    94/100 stars

    Images

    1) Product Images from "Comprehensive Mutation Analysis of the CYP21A2 Gene"

    Article Title: Comprehensive Mutation Analysis of the CYP21A2 Gene

    Journal: The Journal of Molecular Diagnostics : JMD

    doi: 10.1016/j.jmoldx.2013.06.001

    Southern blot analysis of RCCX module in two representative patients and their respective parents. A: Schematic of a typical RCCX bimodule allele. Dashed boxes denote pseudogenes; short vertical lines, TaqI restriction sites; numbers, fragment sizes (in kb). B and C: TaqI and PshAI Southern blots. Enzyme digested fragments with size (in kb) correspond to target gene(s). D: Densitometric ratios were measured in Southern blots. The ratios indicate gene dosages, which can be used to infer gene arrangement. Columns 1 and 1F represent patient 1 and the father, respectively; columns 2, 2M, and 2F represent patient 2, the mother, and the father, respectively.
    Figure Legend Snippet: Southern blot analysis of RCCX module in two representative patients and their respective parents. A: Schematic of a typical RCCX bimodule allele. Dashed boxes denote pseudogenes; short vertical lines, TaqI restriction sites; numbers, fragment sizes (in kb). B and C: TaqI and PshAI Southern blots. Enzyme digested fragments with size (in kb) correspond to target gene(s). D: Densitometric ratios were measured in Southern blots. The ratios indicate gene dosages, which can be used to infer gene arrangement. Columns 1 and 1F represent patient 1 and the father, respectively; columns 2, 2M, and 2F represent patient 2, the mother, and the father, respectively.

    Techniques Used: Southern Blot

    2) Product Images from "Sirtuin 1 Is a Key Regulator of the Interleukin-12 p70/Interleukin-23 Balance in Human Dendritic Cells *"

    Article Title: Sirtuin 1 Is a Key Regulator of the Interleukin-12 p70/Interleukin-23 Balance in Human Dendritic Cells *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M112.391839

    Accessibility of Nuc-1 and Nuc-2 of the il12a promoter. Accessibility was assayed by CHART-PCR. Nuclei were digested with 50 units BstXI or 50 units PshAI for 1 h at 37 °C, and genomic DNA was used to perform SYBR Green quantitative PCR. Amplification with primers for the Nuc-1-encompassing PshAI site is sensitive to remodeling of Nuc-1, and amplification with primers for Nuc-2 encompassing BstXI is sensitive to remodeling of Nuc-2. Results are expressed as a percentage of the undigested sample for each cell treatment as described under “Experimental Procedures.” Results represent the mean ± S.D. of five experiments with triplicate samples. *, p
    Figure Legend Snippet: Accessibility of Nuc-1 and Nuc-2 of the il12a promoter. Accessibility was assayed by CHART-PCR. Nuclei were digested with 50 units BstXI or 50 units PshAI for 1 h at 37 °C, and genomic DNA was used to perform SYBR Green quantitative PCR. Amplification with primers for the Nuc-1-encompassing PshAI site is sensitive to remodeling of Nuc-1, and amplification with primers for Nuc-2 encompassing BstXI is sensitive to remodeling of Nuc-2. Results are expressed as a percentage of the undigested sample for each cell treatment as described under “Experimental Procedures.” Results represent the mean ± S.D. of five experiments with triplicate samples. *, p

    Techniques Used: Polymerase Chain Reaction, SYBR Green Assay, Real-time Polymerase Chain Reaction, Amplification

    Related Articles

    Construct:

    Article Title: A Novel Approach for the Rapid Mutagenesis and Directed Evolution of the Structural Genes of West Nile Virus
    Article Snippet: To increase the utility of pWNV-complement as a template for PCR mutagenesis, two variants were constructed, which encode stop codons that render them defective. pWNV-complement-5′ was constructed by cleaving pWNV-complement with MfeI (which cleaves the structural gene fragment in capsid [at nucleotide 270 of WNV NY99]), followed by blunting with Klenow fragment (New England BioLabs) and intramolecular religation. .. The resulting construct encodes a stop codon in the capsid gene; downstream sequences of this insertion are no longer in frame. pWNV-complement-3′ was constructed by the cleavage of pWNV-complement with PshAI and AleI (removing nucleotides 1920 to 1933 of WNV NY99) (New England BioLabs), followed by intramolecular religation. .. This construct also encodes frameshifting to introduce a stop codon in the E gene; downstream flavivirus sequences in this construct are no longer in frame. pWNV-GFP-backbone was designed by using a configuration described previously ( ).

    Amplification:

    Article Title: Determination of the Loss of Function Complement C4 Exon 29 CT Insertion Using a Novel Paralog-Specific Assay in Healthy UK and Spanish Populations
    Article Snippet: PCR reaction conditions were: initial denaturation step at 94°C for 3 min, followed by 25 cycles at 94°C for 30 sec, 58°C for 30 sec and 72°C for 1 min, followed by a chase phase of 56°C for 1 min and 70°C for 20 min to ensure complete addition of the terminal adenine residue. .. Following amplification, 10 µl PCR product was digested using 5 U of Psh AI restriction enzyme (NEB, Cat. No. R0593L) in 20 µl digestion reaction, comprising 2 µl 10×NEB Buffer 4, 0.2 µl 100× BSA and 7.3 µl of sterile water. ..

    Polymerase Chain Reaction:

    Article Title: Determination of the Loss of Function Complement C4 Exon 29 CT Insertion Using a Novel Paralog-Specific Assay in Healthy UK and Spanish Populations
    Article Snippet: PCR reaction conditions were: initial denaturation step at 94°C for 3 min, followed by 25 cycles at 94°C for 30 sec, 58°C for 30 sec and 72°C for 1 min, followed by a chase phase of 56°C for 1 min and 70°C for 20 min to ensure complete addition of the terminal adenine residue. .. Following amplification, 10 µl PCR product was digested using 5 U of Psh AI restriction enzyme (NEB, Cat. No. R0593L) in 20 µl digestion reaction, comprising 2 µl 10×NEB Buffer 4, 0.2 µl 100× BSA and 7.3 µl of sterile water. ..

    Generated:

    Article Title: Analyses of functional domains within the PF6 protein of the central apparatus reveal a role for PF6 sub-complex members in regulating flagellar beat frequency
    Article Snippet: Digestion of the plasmid with NheI , NsiI , or AatII , followed by circular ligation of the vector containing fragment produced constructs PF6Δ68–752 (PF6ΔN), PF6Δ1459–2301 (PF6ΔC1), and PF6Δ1861–2229 (PF6ΔCB2), respectively. .. PF6Δ854–1821 (PF6ΔM) was generated by double digestion with PshAI and BsrGI , followed by blunting with mung bean nuclease (NEB, Ipswich, MA) and religation. .. PF6Δ68–1760 (PF6ΔN+M) was generated by double digestion with NheI and BsiWI , followed by blunting with mung bean nuclease (NEB) and religation.

    Recombinant:

    Article Title: Sirtuin 1 Is a Key Regulator of the Interleukin-12 p70/Interleukin-23 Balance in Human Dendritic Cells *
    Article Snippet: 3-Aminopyridine adenine dinucleotide (AAD), a NAD+ analog that increases the cellular concentration of NAD+ by inhibiting dehydrogenases , was synthesized from nicotinic acid adenine dinucleotide and sodium azide as described ( ). .. Recombinant histone H3.3, BstXI, and PshAI were from New England BioLabs (Ipswich, MA). p300, pCAF, and SIRT1 recombinant proteins were from Active Motif (Carlsbad, CA). .. SRT1720, a selective activator of SIRT1, was from Cayman Chemical (Ann Arbor, MI).

    other:

    Article Title: Comprehensive Mutation Analysis of the CYP21A2 Gene
    Article Snippet: To genotype the copy number variation of RCCX modules, SB was performed for unrelated patients with 21-OHD and their parents using TaqI and PshAI (both from New England BioLabs, Ipswich, MA) as previously described.

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  • 94
    New England Biolabs pshai
    Southern blot analysis of RCCX module in two representative patients and their respective parents. A: Schematic of a typical RCCX bimodule allele. Dashed boxes denote pseudogenes; short vertical lines, <t>TaqI</t> restriction sites; numbers, fragment sizes (in kb). B and C: TaqI and <t>PshAI</t> Southern blots. Enzyme digested fragments with size (in kb) correspond to target gene(s). D: Densitometric ratios were measured in Southern blots. The ratios indicate gene dosages, which can be used to infer gene arrangement. Columns 1 and 1F represent patient 1 and the father, respectively; columns 2, 2M, and 2F represent patient 2, the mother, and the father, respectively.
    Pshai, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pshai/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    pshai - by Bioz Stars, 2021-06
    94/100 stars
      Buy from Supplier

    Image Search Results


    Southern blot analysis of RCCX module in two representative patients and their respective parents. A: Schematic of a typical RCCX bimodule allele. Dashed boxes denote pseudogenes; short vertical lines, TaqI restriction sites; numbers, fragment sizes (in kb). B and C: TaqI and PshAI Southern blots. Enzyme digested fragments with size (in kb) correspond to target gene(s). D: Densitometric ratios were measured in Southern blots. The ratios indicate gene dosages, which can be used to infer gene arrangement. Columns 1 and 1F represent patient 1 and the father, respectively; columns 2, 2M, and 2F represent patient 2, the mother, and the father, respectively.

    Journal: The Journal of Molecular Diagnostics : JMD

    Article Title: Comprehensive Mutation Analysis of the CYP21A2 Gene

    doi: 10.1016/j.jmoldx.2013.06.001

    Figure Lengend Snippet: Southern blot analysis of RCCX module in two representative patients and their respective parents. A: Schematic of a typical RCCX bimodule allele. Dashed boxes denote pseudogenes; short vertical lines, TaqI restriction sites; numbers, fragment sizes (in kb). B and C: TaqI and PshAI Southern blots. Enzyme digested fragments with size (in kb) correspond to target gene(s). D: Densitometric ratios were measured in Southern blots. The ratios indicate gene dosages, which can be used to infer gene arrangement. Columns 1 and 1F represent patient 1 and the father, respectively; columns 2, 2M, and 2F represent patient 2, the mother, and the father, respectively.

    Article Snippet: To genotype the copy number variation of RCCX modules, SB was performed for unrelated patients with 21-OHD and their parents using TaqI and PshAI (both from New England BioLabs, Ipswich, MA) as previously described.

    Techniques: Southern Blot

    Schematic organization of JcDV-based plasmids used to generate linear sequences for transfection experiments. pBR322 backbone is figured as dotted gray line. JcDV structural proteins ( VP ) coding sequences are represented by a solid black line; non-structural proteins ( NS ) genes by a solid gray line. Both share a polyadenylation signal shown as an open ellipse. Open boxes figure the p9 and p93 ITRs, hatched boxes underline the location of p9 and p93 promoters, respectively. GFP coding sequence is figured with a gray box and its 3′ SV40-derived polyadenylation signal is shown as a hatched line. Arrows numbered according to Table 1 figure the primers used for PCR-based experiments. Primers used for walk-PCR are represented above; primers 7–13 represented only relatively to p9 can also hybridize to p93 DNA sequences when present. Gray-filled arrowheads figure restriction sites used to generate linear molecules from the plasmid constructs. Open arrowheads figure restriction sites used for walk-PCR experiments. Subscript numbers indicate iterated restriction sites. By convention, nucleotide numbers of each linear molecule are accorded to the 5′ C generated after Cla I restriction (AT/CGAT). Restriction enzymes are: A: Afl II, C: Cla I, D: Dra I, H: Hpa I, M: Msp A1L, S: Ssp I, P: Psh AI, Pv: Pvu II, X: Xcm I, respectively. Linear molecules were obtained after restriction of three different JcDV-based vectors. Their length is indicated under the name of each plasmid: (A) pFull encompassing a full-length sequence of JcDV DNA and the GFP marker gene, cloned into pBR322 plasmid. This schematic representation displays all the symbols described above; some of them only are reported in B and C. (B) pVP in comparison to pFull, a frameshift deletion affects the NS region (C) pNS in comparison to pFull, lacks VP genes. The expression of GFP is directly under the control of the p9 promoter. Primers giving rise to specific products after PCR are shown (See Table 1 ).

    Journal: PeerJ

    Article Title: Linear Lepidopteran ambidensovirus 1 sequences drive random integration of a reporter gene in transfected Spodoptera frugiperda cells

    doi: 10.7717/peerj.4860

    Figure Lengend Snippet: Schematic organization of JcDV-based plasmids used to generate linear sequences for transfection experiments. pBR322 backbone is figured as dotted gray line. JcDV structural proteins ( VP ) coding sequences are represented by a solid black line; non-structural proteins ( NS ) genes by a solid gray line. Both share a polyadenylation signal shown as an open ellipse. Open boxes figure the p9 and p93 ITRs, hatched boxes underline the location of p9 and p93 promoters, respectively. GFP coding sequence is figured with a gray box and its 3′ SV40-derived polyadenylation signal is shown as a hatched line. Arrows numbered according to Table 1 figure the primers used for PCR-based experiments. Primers used for walk-PCR are represented above; primers 7–13 represented only relatively to p9 can also hybridize to p93 DNA sequences when present. Gray-filled arrowheads figure restriction sites used to generate linear molecules from the plasmid constructs. Open arrowheads figure restriction sites used for walk-PCR experiments. Subscript numbers indicate iterated restriction sites. By convention, nucleotide numbers of each linear molecule are accorded to the 5′ C generated after Cla I restriction (AT/CGAT). Restriction enzymes are: A: Afl II, C: Cla I, D: Dra I, H: Hpa I, M: Msp A1L, S: Ssp I, P: Psh AI, Pv: Pvu II, X: Xcm I, respectively. Linear molecules were obtained after restriction of three different JcDV-based vectors. Their length is indicated under the name of each plasmid: (A) pFull encompassing a full-length sequence of JcDV DNA and the GFP marker gene, cloned into pBR322 plasmid. This schematic representation displays all the symbols described above; some of them only are reported in B and C. (B) pVP in comparison to pFull, a frameshift deletion affects the NS region (C) pNS in comparison to pFull, lacks VP genes. The expression of GFP is directly under the control of the p9 promoter. Primers giving rise to specific products after PCR are shown (See Table 1 ).

    Article Snippet: DNA was cut as indicated in with restriction enzymes Cla I, Pvu II, Xcm I, Afl II, Msp A1L and Psh AI according to the New England Biolabs protocols.

    Techniques: Transfection, Sequencing, Derivative Assay, Polymerase Chain Reaction, Plasmid Preparation, Construct, Generated, Marker, Clone Assay, Expressing

    Accessibility of Nuc-1 and Nuc-2 of the il12a promoter. Accessibility was assayed by CHART-PCR. Nuclei were digested with 50 units BstXI or 50 units PshAI for 1 h at 37 °C, and genomic DNA was used to perform SYBR Green quantitative PCR. Amplification with primers for the Nuc-1-encompassing PshAI site is sensitive to remodeling of Nuc-1, and amplification with primers for Nuc-2 encompassing BstXI is sensitive to remodeling of Nuc-2. Results are expressed as a percentage of the undigested sample for each cell treatment as described under “Experimental Procedures.” Results represent the mean ± S.D. of five experiments with triplicate samples. *, p

    Journal: The Journal of Biological Chemistry

    Article Title: Sirtuin 1 Is a Key Regulator of the Interleukin-12 p70/Interleukin-23 Balance in Human Dendritic Cells *

    doi: 10.1074/jbc.M112.391839

    Figure Lengend Snippet: Accessibility of Nuc-1 and Nuc-2 of the il12a promoter. Accessibility was assayed by CHART-PCR. Nuclei were digested with 50 units BstXI or 50 units PshAI for 1 h at 37 °C, and genomic DNA was used to perform SYBR Green quantitative PCR. Amplification with primers for the Nuc-1-encompassing PshAI site is sensitive to remodeling of Nuc-1, and amplification with primers for Nuc-2 encompassing BstXI is sensitive to remodeling of Nuc-2. Results are expressed as a percentage of the undigested sample for each cell treatment as described under “Experimental Procedures.” Results represent the mean ± S.D. of five experiments with triplicate samples. *, p

    Article Snippet: Recombinant histone H3.3, BstXI, and PshAI were from New England BioLabs (Ipswich, MA). p300, pCAF, and SIRT1 recombinant proteins were from Active Motif (Carlsbad, CA).

    Techniques: Polymerase Chain Reaction, SYBR Green Assay, Real-time Polymerase Chain Reaction, Amplification

    Defining the RCCX modular structure using different genomic RFLP Southern blot analyses. A, Restriction patterns of Taq I RFLP for the five selected individuals on simultaneous hybridization of probes specific for 3′ RP, CYP21, and 3′ TNX. B, Psh AI RFLP hybridized to a 3′ RP probe. C, Bam HI RFLP hybridized to a 3′ TNX probe. D, Phenotyping of C4A and C4B proteins by immunofixation of EDTA-blood plasma resolved by HVAGE. E, Immunoblot analysis of Rg1 and Ch1 antigenic determinants in plasma C4 proteins after HVAGE. F, Genomic Southern blot analysis of C4A and C4B genes by Psh AI-RFLP (using Probe D) and by Psh AI- Pvu II RFLP (using probe 22–25). G, HLA class I and class II alleles of the five subjects.

    Journal: American Journal of Human Genetics

    Article Title: Determining the One, Two, Three, or Four Long and Short Loci of Human Complement C4 in a Major Histocompatibility Complex Haplotype Encoding C4A or C4B Proteins

    doi:

    Figure Lengend Snippet: Defining the RCCX modular structure using different genomic RFLP Southern blot analyses. A, Restriction patterns of Taq I RFLP for the five selected individuals on simultaneous hybridization of probes specific for 3′ RP, CYP21, and 3′ TNX. B, Psh AI RFLP hybridized to a 3′ RP probe. C, Bam HI RFLP hybridized to a 3′ TNX probe. D, Phenotyping of C4A and C4B proteins by immunofixation of EDTA-blood plasma resolved by HVAGE. E, Immunoblot analysis of Rg1 and Ch1 antigenic determinants in plasma C4 proteins after HVAGE. F, Genomic Southern blot analysis of C4A and C4B genes by Psh AI-RFLP (using Probe D) and by Psh AI- Pvu II RFLP (using probe 22–25). G, HLA class I and class II alleles of the five subjects.

    Article Snippet: Restriction enzymes included Taq I, Psh AI (New England Biolabs), and Pvu II (Invitrogen).

    Techniques: Southern Blot, Hybridization