fsei (New England Biolabs)


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Fsei, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/fsei/product/New England Biolabs
Average 95 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "Extensive amplification of GI-VII-6, a multidrug resistance genomic island of Salmonella enterica serovar Typhimurium, increases resistance to extended-spectrum cephalosporins"
Article Title: Extensive amplification of GI-VII-6, a multidrug resistance genomic island of Salmonella enterica serovar Typhimurium, increases resistance to extended-spectrum cephalosporins
Journal: Frontiers in Microbiology
doi: 10.3389/fmicb.2015.00078

Figure Legend Snippet: PFGE–Southern hybridization images demonstrating bla CMY−2 and spvB locations in S . Typhimurium strains. (A) PFGE separation of S1 nuclease-digested genomic DNA from the selected strains followed by Southern hybridization with probes specific to bla CMY−2 and spvB . An arrow indicates the position of the endogenous 133-kb plasmid, in which the spvB signal was detected. Open triangles indicate the positions of enlarged plasmids, in which both bla CMY−2 and spvB signals were detected. Both signals were observed in multiple plasmid bands in strains 12-13, 12-14, and 12-18. (B) PFGE separation of FseI-digested genomic DNA from the selected strains followed by Southern hybridization with a bla CMY−2 probe. An arrow indicates the original position of the restricted fragment (350 kb) in which the chromosomal bla CMY−2 signal was detected. In the selected mutants, this fragment was not present and the bla CMY−2 signal was identified on the largest band as indicated by a closed trangle. Open triangles indicate the position of enlarged plasmid observed in strains 12-14, 25-6, and 25-17. (C) PFGE separation of XbaI-digested genomic DNA from the selected strains followed by Southern hybridization with a bla CMY−2 probe. An arrow indicates the original position of the restricted fragment (520 kb) in which chromosomal bla CMY−2 signal was detected. Closed triangles indicate the position of bla CMY−2 signals that originated from tandemly amplified GI-VII-6. (D) Restriction maps of the GI-VII-6 and its flanking region in the parent strain L-3553 and the mutants with putative amplifications. One putative amplified region presented is flanked by the first and the third copies of IS 26 (upper), and the other is flanked by the first and the fourth copies of IS 26 (lower). These amplified regions generate 100-kb (upper) and 125-kb (lower) fragments after XbaI digestion, respectively.
Techniques Used: Hybridization, Plasmid Preparation, Amplification