fsei  (New England Biolabs)


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    New England Biolabs fsei
    PFGE–Southern hybridization images demonstrating bla CMY−2 and spvB locations in S . Typhimurium strains. (A) PFGE separation of S1 nuclease-digested genomic DNA from the selected strains followed by Southern hybridization with probes specific to bla CMY−2 and spvB . An arrow indicates the position of the endogenous 133-kb plasmid, in which the spvB signal was detected. Open triangles indicate the positions of enlarged plasmids, in which both bla CMY−2 and spvB signals were detected. Both signals were observed in multiple plasmid bands in strains 12-13, 12-14, and 12-18. (B) PFGE separation of <t>FseI-digested</t> genomic DNA from the selected strains followed by Southern hybridization with a bla CMY−2 probe. An arrow indicates the original position of the restricted fragment (350 kb) in which the chromosomal bla CMY−2 signal was detected. In the selected mutants, this fragment was not present and the bla CMY−2 signal was identified on the largest band as indicated by a closed trangle. Open triangles indicate the position of enlarged plasmid observed in strains 12-14, 25-6, and 25-17. (C) PFGE separation of <t>XbaI-digested</t> genomic DNA from the selected strains followed by Southern hybridization with a bla CMY−2 probe. An arrow indicates the original position of the restricted fragment (520 kb) in which chromosomal bla CMY−2 signal was detected. Closed triangles indicate the position of bla CMY−2 signals that originated from tandemly amplified GI-VII-6. (D) Restriction maps of the GI-VII-6 and its flanking region in the parent strain L-3553 and the mutants with putative amplifications. One putative amplified region presented is flanked by the first and the third copies of IS 26 (upper), and the other is flanked by the first and the fourth copies of IS 26 (lower). These amplified regions generate 100-kb (upper) and 125-kb (lower) fragments after XbaI digestion, respectively.
    Fsei, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Extensive amplification of GI-VII-6, a multidrug resistance genomic island of Salmonella enterica serovar Typhimurium, increases resistance to extended-spectrum cephalosporins"

    Article Title: Extensive amplification of GI-VII-6, a multidrug resistance genomic island of Salmonella enterica serovar Typhimurium, increases resistance to extended-spectrum cephalosporins

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2015.00078

    PFGE–Southern hybridization images demonstrating bla CMY−2 and spvB locations in S . Typhimurium strains. (A) PFGE separation of S1 nuclease-digested genomic DNA from the selected strains followed by Southern hybridization with probes specific to bla CMY−2 and spvB . An arrow indicates the position of the endogenous 133-kb plasmid, in which the spvB signal was detected. Open triangles indicate the positions of enlarged plasmids, in which both bla CMY−2 and spvB signals were detected. Both signals were observed in multiple plasmid bands in strains 12-13, 12-14, and 12-18. (B) PFGE separation of FseI-digested genomic DNA from the selected strains followed by Southern hybridization with a bla CMY−2 probe. An arrow indicates the original position of the restricted fragment (350 kb) in which the chromosomal bla CMY−2 signal was detected. In the selected mutants, this fragment was not present and the bla CMY−2 signal was identified on the largest band as indicated by a closed trangle. Open triangles indicate the position of enlarged plasmid observed in strains 12-14, 25-6, and 25-17. (C) PFGE separation of XbaI-digested genomic DNA from the selected strains followed by Southern hybridization with a bla CMY−2 probe. An arrow indicates the original position of the restricted fragment (520 kb) in which chromosomal bla CMY−2 signal was detected. Closed triangles indicate the position of bla CMY−2 signals that originated from tandemly amplified GI-VII-6. (D) Restriction maps of the GI-VII-6 and its flanking region in the parent strain L-3553 and the mutants with putative amplifications. One putative amplified region presented is flanked by the first and the third copies of IS 26 (upper), and the other is flanked by the first and the fourth copies of IS 26 (lower). These amplified regions generate 100-kb (upper) and 125-kb (lower) fragments after XbaI digestion, respectively.
    Figure Legend Snippet: PFGE–Southern hybridization images demonstrating bla CMY−2 and spvB locations in S . Typhimurium strains. (A) PFGE separation of S1 nuclease-digested genomic DNA from the selected strains followed by Southern hybridization with probes specific to bla CMY−2 and spvB . An arrow indicates the position of the endogenous 133-kb plasmid, in which the spvB signal was detected. Open triangles indicate the positions of enlarged plasmids, in which both bla CMY−2 and spvB signals were detected. Both signals were observed in multiple plasmid bands in strains 12-13, 12-14, and 12-18. (B) PFGE separation of FseI-digested genomic DNA from the selected strains followed by Southern hybridization with a bla CMY−2 probe. An arrow indicates the original position of the restricted fragment (350 kb) in which the chromosomal bla CMY−2 signal was detected. In the selected mutants, this fragment was not present and the bla CMY−2 signal was identified on the largest band as indicated by a closed trangle. Open triangles indicate the position of enlarged plasmid observed in strains 12-14, 25-6, and 25-17. (C) PFGE separation of XbaI-digested genomic DNA from the selected strains followed by Southern hybridization with a bla CMY−2 probe. An arrow indicates the original position of the restricted fragment (520 kb) in which chromosomal bla CMY−2 signal was detected. Closed triangles indicate the position of bla CMY−2 signals that originated from tandemly amplified GI-VII-6. (D) Restriction maps of the GI-VII-6 and its flanking region in the parent strain L-3553 and the mutants with putative amplifications. One putative amplified region presented is flanked by the first and the third copies of IS 26 (upper), and the other is flanked by the first and the fourth copies of IS 26 (lower). These amplified regions generate 100-kb (upper) and 125-kb (lower) fragments after XbaI digestion, respectively.

    Techniques Used: Hybridization, Plasmid Preparation, Amplification

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    New England Biolabs fsei
    PFGE–Southern hybridization images demonstrating bla CMY−2 and spvB locations in S . Typhimurium strains. (A) PFGE separation of S1 nuclease-digested genomic DNA from the selected strains followed by Southern hybridization with probes specific to bla CMY−2 and spvB . An arrow indicates the position of the endogenous 133-kb plasmid, in which the spvB signal was detected. Open triangles indicate the positions of enlarged plasmids, in which both bla CMY−2 and spvB signals were detected. Both signals were observed in multiple plasmid bands in strains 12-13, 12-14, and 12-18. (B) PFGE separation of <t>FseI-digested</t> genomic DNA from the selected strains followed by Southern hybridization with a bla CMY−2 probe. An arrow indicates the original position of the restricted fragment (350 kb) in which the chromosomal bla CMY−2 signal was detected. In the selected mutants, this fragment was not present and the bla CMY−2 signal was identified on the largest band as indicated by a closed trangle. Open triangles indicate the position of enlarged plasmid observed in strains 12-14, 25-6, and 25-17. (C) PFGE separation of <t>XbaI-digested</t> genomic DNA from the selected strains followed by Southern hybridization with a bla CMY−2 probe. An arrow indicates the original position of the restricted fragment (520 kb) in which chromosomal bla CMY−2 signal was detected. Closed triangles indicate the position of bla CMY−2 signals that originated from tandemly amplified GI-VII-6. (D) Restriction maps of the GI-VII-6 and its flanking region in the parent strain L-3553 and the mutants with putative amplifications. One putative amplified region presented is flanked by the first and the third copies of IS 26 (upper), and the other is flanked by the first and the fourth copies of IS 26 (lower). These amplified regions generate 100-kb (upper) and 125-kb (lower) fragments after XbaI digestion, respectively.
    Fsei, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fsei/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fsei - by Bioz Stars, 2022-07
    95/100 stars
    		  Buy from Supplier

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    PFGE–Southern hybridization images demonstrating bla CMY−2 and spvB locations in S . Typhimurium strains. (A) PFGE separation of S1 nuclease-digested genomic DNA from the selected strains followed by Southern hybridization with probes specific to bla CMY−2 and spvB . An arrow indicates the position of the endogenous 133-kb plasmid, in which the spvB signal was detected. Open triangles indicate the positions of enlarged plasmids, in which both bla CMY−2 and spvB signals were detected. Both signals were observed in multiple plasmid bands in strains 12-13, 12-14, and 12-18. (B) PFGE separation of FseI-digested genomic DNA from the selected strains followed by Southern hybridization with a bla CMY−2 probe. An arrow indicates the original position of the restricted fragment (350 kb) in which the chromosomal bla CMY−2 signal was detected. In the selected mutants, this fragment was not present and the bla CMY−2 signal was identified on the largest band as indicated by a closed trangle. Open triangles indicate the position of enlarged plasmid observed in strains 12-14, 25-6, and 25-17. (C) PFGE separation of XbaI-digested genomic DNA from the selected strains followed by Southern hybridization with a bla CMY−2 probe. An arrow indicates the original position of the restricted fragment (520 kb) in which chromosomal bla CMY−2 signal was detected. Closed triangles indicate the position of bla CMY−2 signals that originated from tandemly amplified GI-VII-6. (D) Restriction maps of the GI-VII-6 and its flanking region in the parent strain L-3553 and the mutants with putative amplifications. One putative amplified region presented is flanked by the first and the third copies of IS 26 (upper), and the other is flanked by the first and the fourth copies of IS 26 (lower). These amplified regions generate 100-kb (upper) and 125-kb (lower) fragments after XbaI digestion, respectively.

    Journal: Frontiers in Microbiology

    Article Title: Extensive amplification of GI-VII-6, a multidrug resistance genomic island of Salmonella enterica serovar Typhimurium, increases resistance to extended-spectrum cephalosporins

    doi: 10.3389/fmicb.2015.00078

    Figure Lengend Snippet: PFGE–Southern hybridization images demonstrating bla CMY−2 and spvB locations in S . Typhimurium strains. (A) PFGE separation of S1 nuclease-digested genomic DNA from the selected strains followed by Southern hybridization with probes specific to bla CMY−2 and spvB . An arrow indicates the position of the endogenous 133-kb plasmid, in which the spvB signal was detected. Open triangles indicate the positions of enlarged plasmids, in which both bla CMY−2 and spvB signals were detected. Both signals were observed in multiple plasmid bands in strains 12-13, 12-14, and 12-18. (B) PFGE separation of FseI-digested genomic DNA from the selected strains followed by Southern hybridization with a bla CMY−2 probe. An arrow indicates the original position of the restricted fragment (350 kb) in which the chromosomal bla CMY−2 signal was detected. In the selected mutants, this fragment was not present and the bla CMY−2 signal was identified on the largest band as indicated by a closed trangle. Open triangles indicate the position of enlarged plasmid observed in strains 12-14, 25-6, and 25-17. (C) PFGE separation of XbaI-digested genomic DNA from the selected strains followed by Southern hybridization with a bla CMY−2 probe. An arrow indicates the original position of the restricted fragment (520 kb) in which chromosomal bla CMY−2 signal was detected. Closed triangles indicate the position of bla CMY−2 signals that originated from tandemly amplified GI-VII-6. (D) Restriction maps of the GI-VII-6 and its flanking region in the parent strain L-3553 and the mutants with putative amplifications. One putative amplified region presented is flanked by the first and the third copies of IS 26 (upper), and the other is flanked by the first and the fourth copies of IS 26 (lower). These amplified regions generate 100-kb (upper) and 125-kb (lower) fragments after XbaI digestion, respectively.

    Article Snippet: The plugs were digested with 60 U/ml of XbaI (Takara Bio Inc.) or 40 U/ml of FseI (New England BioLabs, Ipswich, MA, USA) at 37°C for 6 h, or 2 U/ml of S1 nuclease (Takara Bio Inc.) at 37°C for 45 min. Primers used for probe synthesis were listed in Table .

    Techniques: Hybridization, Plasmid Preparation, Amplification

    (A) The flank area with enzymes sequences (yellow: Bgl II, gray: Swa I, pink: Kpn I, orange: Fse I, violet: Bsr gI). (B) The map of the pGEM-b1 vector. (C) pGEM-b1 vector that contain the flank regions and specific restricted enzymes for cloning. (D) The pLexsy-2 vector contain construct after cloning

    Journal: Iranian Journal of Parasitology

    Article Title: Designing and Cloning Molecular Constructs to Knock Out N-Acetylglucosamine Phosphatidylinositol De-N-Acetylase (GPI12) Gene in Leishmania major (MRHO/IR/75/ER)

    doi:

    Figure Lengend Snippet: (A) The flank area with enzymes sequences (yellow: Bgl II, gray: Swa I, pink: Kpn I, orange: Fse I, violet: Bsr gI). (B) The map of the pGEM-b1 vector. (C) pGEM-b1 vector that contain the flank regions and specific restricted enzymes for cloning. (D) The pLexsy-2 vector contain construct after cloning

    Article Snippet: The PCR fragments were cut by Fse I and Bsr gI enzymes (New England Biolabs, UK).

    Techniques: Plasmid Preparation, Clone Assay, Construct

    In all figures, lane 1 shows molecular size marker. (A) The sequences of flank areas on which PCR was performed by primerM13; Lane 2 shows the amplified ∼1kb band. (B) The PCR fragments which were cut with Bgl II and Kpn I enzymes. The PCR product which was divided into 3 pieces of 600, 300 and 100 bp; Lane 2 shows the 300 bp fragment. (C) The pLEXSY vector, which was cut by Fse I and Bsr gI enzymes; Lane 2 shows the ∼ 0.7 kb fragment is separated from the vector. (D) The pLEXSY vector, which was cut by Bgl II and Kpn I enzymes. Lane 2 shows the ∼1 kb fragment is separated from the vector. (E) The PCR fragments which were cut with Fse I and Bsr gI enzymes. The PCR product which was divided into 3 pieces of 500, 400 and 100 bp; Lane 2 shows the 500 bp fragment

    Journal: Iranian Journal of Parasitology

    Article Title: Designing and Cloning Molecular Constructs to Knock Out N-Acetylglucosamine Phosphatidylinositol De-N-Acetylase (GPI12) Gene in Leishmania major (MRHO/IR/75/ER)

    doi:

    Figure Lengend Snippet: In all figures, lane 1 shows molecular size marker. (A) The sequences of flank areas on which PCR was performed by primerM13; Lane 2 shows the amplified ∼1kb band. (B) The PCR fragments which were cut with Bgl II and Kpn I enzymes. The PCR product which was divided into 3 pieces of 600, 300 and 100 bp; Lane 2 shows the 300 bp fragment. (C) The pLEXSY vector, which was cut by Fse I and Bsr gI enzymes; Lane 2 shows the ∼ 0.7 kb fragment is separated from the vector. (D) The pLEXSY vector, which was cut by Bgl II and Kpn I enzymes. Lane 2 shows the ∼1 kb fragment is separated from the vector. (E) The PCR fragments which were cut with Fse I and Bsr gI enzymes. The PCR product which was divided into 3 pieces of 500, 400 and 100 bp; Lane 2 shows the 500 bp fragment

    Article Snippet: The PCR fragments were cut by Fse I and Bsr gI enzymes (New England Biolabs, UK).

    Techniques: Marker, Polymerase Chain Reaction, Amplification, Plasmid Preparation

    Preparation of the reporter gene GFPCreERT2. A: Diagram of the shuttle vector carrying GFPCreERT2; B: Electrophoretogram of the shuttle vector cut by Not I and Fse I; C: Electrophoretogram after the GFPCreERT2 fragment was cut from the gel. M: Marker; Gpm6a: Shuttle vector inserted by the 5arm and 3arm from the Gpm6a BAC; Reelin: Shuttle vector inserted by the 5arm and 3arm from the Reelin BAC.

    Journal: World Journal of Gastroenterology

    Article Title: Construction of Gpm6a/ReelinGFPCreERT2 by BAC recombination using a specific gene in hepatic mesothelial or stellate cells

    doi: 10.3748/wjg.v23.i2.224

    Figure Lengend Snippet: Preparation of the reporter gene GFPCreERT2. A: Diagram of the shuttle vector carrying GFPCreERT2; B: Electrophoretogram of the shuttle vector cut by Not I and Fse I; C: Electrophoretogram after the GFPCreERT2 fragment was cut from the gel. M: Marker; Gpm6a: Shuttle vector inserted by the 5arm and 3arm from the Gpm6a BAC; Reelin: Shuttle vector inserted by the 5arm and 3arm from the Reelin BAC.

    Article Snippet: The vector was digested with Not I and Fse I (New England Biolabs), and electrophoresis was performed with agarose gels (Takara, Osaka, Japan).

    Techniques: Plasmid Preparation, Marker, BAC Assay