pcr  (New England Biolabs)


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  • 99
    Name:
    BssS alpha I
    Description:
    BssS alpha I 1 000 units
    Catalog Number:
    R0680L
    Price:
    302
    Size:
    1 000 units
    Category:
    Restriction Enzymes
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    Structured Review

    New England Biolabs pcr
    BssS alpha I
    BssS alpha I 1 000 units
    https://www.bioz.com/result/pcr/product/New England Biolabs
    Average 99 stars, based on 12234 article reviews
    Price from $9.99 to $1999.99
    pcr - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: The planar cell polarity protein VANG-1/Vangl negatively regulates Wnt/β-catenin signaling through a Dvl dependent mechanism
    Article Snippet: The resulting PCR product was subsequently digested with BsoBI (R0586, NEB) for Vangl1 and BssSI (R0587, NEB) for Vangl2 to detect disruption of gRNA targeted restriction sites. .. The positive clones were selected for further screening by whole-cell lysate western blot analysis.

    Transfection:

    Article Title: The planar cell polarity protein VANG-1/Vangl negatively regulates Wnt/β-catenin signaling through a Dvl dependent mechanism
    Article Snippet: Plasmids were transiently transfected using Lipofectamine 2000 (Invitrogen) into T-REx 293 cells. .. The resulting PCR product was subsequently digested with BsoBI (R0586, NEB) for Vangl1 and BssSI (R0587, NEB) for Vangl2 to detect disruption of gRNA targeted restriction sites.

    Amplification:

    Article Title: The planar cell polarity protein VANG-1/Vangl negatively regulates Wnt/β-catenin signaling through a Dvl dependent mechanism
    Article Snippet: Isolated gDNA was used for amplification of a region surrounding the place of Cas9 cutting. .. The resulting PCR product was subsequently digested with BsoBI (R0586, NEB) for Vangl1 and BssSI (R0587, NEB) for Vangl2 to detect disruption of gRNA targeted restriction sites.

    Isolation:

    Article Title: The planar cell polarity protein VANG-1/Vangl negatively regulates Wnt/β-catenin signaling through a Dvl dependent mechanism
    Article Snippet: Isolated gDNA was used for amplification of a region surrounding the place of Cas9 cutting. .. The resulting PCR product was subsequently digested with BsoBI (R0586, NEB) for Vangl1 and BssSI (R0587, NEB) for Vangl2 to detect disruption of gRNA targeted restriction sites.

    CRISPR:

    Article Title: The planar cell polarity protein VANG-1/Vangl negatively regulates Wnt/β-catenin signaling through a Dvl dependent mechanism
    Article Snippet: Paragraph title: CRISPR/Cas9 mediated targeting of Vangl1 and Vangl2 ... The resulting PCR product was subsequently digested with BsoBI (R0586, NEB) for Vangl1 and BssSI (R0587, NEB) for Vangl2 to detect disruption of gRNA targeted restriction sites.

    Polymerase Chain Reaction:

    Article Title: The planar cell polarity protein VANG-1/Vangl negatively regulates Wnt/β-catenin signaling through a Dvl dependent mechanism
    Article Snippet: .. The resulting PCR product was subsequently digested with BsoBI (R0586, NEB) for Vangl1 and BssSI (R0587, NEB) for Vangl2 to detect disruption of gRNA targeted restriction sites. .. The positive clones were selected for further screening by whole-cell lysate western blot analysis.

    other:

    Article Title: Self-catalyzed Site-specific Depurination of G Residues Mediated by Cruciform Extrusion in Closed Circular DNA Plasmids *
    Article Snippet: Restriction endonucleases BamHI, HinfIII, NdeI, and BssSI were from New England Biolabs (NEB), and digests were conducted according to the supplier's instructions.

    Western Blot:

    Article Title: The planar cell polarity protein VANG-1/Vangl negatively regulates Wnt/β-catenin signaling through a Dvl dependent mechanism
    Article Snippet: The resulting PCR product was subsequently digested with BsoBI (R0586, NEB) for Vangl1 and BssSI (R0587, NEB) for Vangl2 to detect disruption of gRNA targeted restriction sites. .. The positive clones were selected for further screening by whole-cell lysate western blot analysis.

    Sequencing:

    Article Title: The planar cell polarity protein VANG-1/Vangl negatively regulates Wnt/β-catenin signaling through a Dvl dependent mechanism
    Article Snippet: The resulting PCR product was subsequently digested with BsoBI (R0586, NEB) for Vangl1 and BssSI (R0587, NEB) for Vangl2 to detect disruption of gRNA targeted restriction sites. .. Final screening of clones was performed by sequence analysis using the Illumina platform and compared with the reference sequence [ ].

    FACS:

    Article Title: The planar cell polarity protein VANG-1/Vangl negatively regulates Wnt/β-catenin signaling through a Dvl dependent mechanism
    Article Snippet: After 24 hr, cells were trypsinized, FACS sorted, and GFP and mCherry double positive cells were plated as single cells in 96-well plates. .. The resulting PCR product was subsequently digested with BsoBI (R0586, NEB) for Vangl1 and BssSI (R0587, NEB) for Vangl2 to detect disruption of gRNA targeted restriction sites.

    Lysis:

    Article Title: The planar cell polarity protein VANG-1/Vangl negatively regulates Wnt/β-catenin signaling through a Dvl dependent mechanism
    Article Snippet: Part of the cellular suspension was taken for genomic DNA (gDNA) isolation, while the rest of the cells was further expanded. gDNA isolation was performed using DirectPCR Lysis Reagent (Cell) (Viagen Biotech), Proteinase K (EO0491, Thermo Fisher Scientific) and DreamTaq DNA Polymerase (EP0701, Thermo Fisher Scientific) according to the manufacturer’s recommendations. .. The resulting PCR product was subsequently digested with BsoBI (R0586, NEB) for Vangl1 and BssSI (R0587, NEB) for Vangl2 to detect disruption of gRNA targeted restriction sites.

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  • 99
    New England Biolabs bso bi
    Bso Bi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bso bi/product/New England Biolabs
    Average 99 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    bso bi - by Bioz Stars, 2020-04
    99/100 stars
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