tspri  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95
    Name:
    TspRI
    Description:
    TspRI 5 000 units
    Catalog Number:
    R0582L
    Price:
    307
    Category:
    Restriction Enzymes
    Size:
    5 000 units
    Buy from Supplier


    Structured Review

    New England Biolabs tspri
    TspRI
    TspRI 5 000 units
    https://www.bioz.com/result/tspri/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tspri - by Bioz Stars, 2021-06
    95/100 stars

    Images

    1) Product Images from "BRAF V600 mutations in Langerhans cell histiocytosis with a simple and unique assay"

    Article Title: BRAF V600 mutations in Langerhans cell histiocytosis with a simple and unique assay

    Journal: Diagnostic Pathology

    doi: 10.1186/s13000-016-0489-z

    CD1a immunohistochemistry FFPE tissue section from LCH patients stained with CD1a. ( a ) Two samples that were V600E mutation (+) after both primary PCR amplification and TspRI followed by additional PCR amplification. Four of five samples in this category demonstrated up to 70 % tumor content. These cases were easy to detect by primary PCR. ( b ) Examples of staining of samples that were BRAF mutation (-) after primary PCR, but mutation (+) after TspRI treatment. Seven of eight such samples contained
    Figure Legend Snippet: CD1a immunohistochemistry FFPE tissue section from LCH patients stained with CD1a. ( a ) Two samples that were V600E mutation (+) after both primary PCR amplification and TspRI followed by additional PCR amplification. Four of five samples in this category demonstrated up to 70 % tumor content. These cases were easy to detect by primary PCR. ( b ) Examples of staining of samples that were BRAF mutation (-) after primary PCR, but mutation (+) after TspRI treatment. Seven of eight such samples contained

    Techniques Used: Immunohistochemistry, Formalin-fixed Paraffin-Embedded, Staining, Mutagenesis, Polymerase Chain Reaction, Amplification

    The effect of TspRI restriction enzyme treatment on the BRAF sequence analysis of patient samples ( a ) Patient no. 21, representative sample with no V600E mutation, was judged mutation (-):GTG after primary PCR, and this conclusion was not changed by treatment with TspRI followed by secondary PCR; ( b ) patient no. 23 was scored as mutation (-):GTG after primary PCR, but mutation (+):GAG after treatment with TspRI; ( c ) the mutation status of patient no. 6 was difficult to judge from the primary PCR because of overlap between mutation (-):GTG and mutation (+):GAG sequences, however, after treatment with TspRI, the mutation (+):GAG sequence was dominant, indicating a clear mutation (+):GAG status; ( d ) patient no. 3 was initially judged mutation (-):GTG after primary PCR, but mutation (+):GAT status was apparent after treatment with TspRI
    Figure Legend Snippet: The effect of TspRI restriction enzyme treatment on the BRAF sequence analysis of patient samples ( a ) Patient no. 21, representative sample with no V600E mutation, was judged mutation (-):GTG after primary PCR, and this conclusion was not changed by treatment with TspRI followed by secondary PCR; ( b ) patient no. 23 was scored as mutation (-):GTG after primary PCR, but mutation (+):GAG after treatment with TspRI; ( c ) the mutation status of patient no. 6 was difficult to judge from the primary PCR because of overlap between mutation (-):GTG and mutation (+):GAG sequences, however, after treatment with TspRI, the mutation (+):GAG sequence was dominant, indicating a clear mutation (+):GAG status; ( d ) patient no. 3 was initially judged mutation (-):GTG after primary PCR, but mutation (+):GAT status was apparent after treatment with TspRI

    Techniques Used: Sequencing, Mutagenesis, Polymerase Chain Reaction

    Optimization of TspRI digestion for the BRAF amplicons. 100 ng of BRAF PCR fragments amplified from normal DNA were digested with TspRI. Lane 1: No TspRI, 65 °C, 15 min. Lane 2: 5U TspRI, 65 °C, 5 min. Lane 3: 10U TspRI, 65 °C, 10 min. Lane 4: 20U TspRI, 65 °C, 15 min, leading to digestion of the majority of BRAF PCR fragments
    Figure Legend Snippet: Optimization of TspRI digestion for the BRAF amplicons. 100 ng of BRAF PCR fragments amplified from normal DNA were digested with TspRI. Lane 1: No TspRI, 65 °C, 15 min. Lane 2: 5U TspRI, 65 °C, 5 min. Lane 3: 10U TspRI, 65 °C, 10 min. Lane 4: 20U TspRI, 65 °C, 15 min, leading to digestion of the majority of BRAF PCR fragments

    Techniques Used: Polymerase Chain Reaction, Amplification

    2) Product Images from "Efficient amplification of self-gelling polypod-like structured DNA by rolling circle amplification and enzymatic digestion"

    Article Title: Efficient amplification of self-gelling polypod-like structured DNA by rolling circle amplification and enzymatic digestion

    Journal: Scientific Reports

    doi: 10.1038/srep14979

    Schematic diagram of the mass amplification of tripodna with adhesive 5′-ends. The template oligodeoxynucleotides (template 1) were designed to satisfy the following requirements: ( a ) the polypodna automatically forms by self-assembly; ( b ) each pod of the polypodna contains a 9 base long TspRI restriction digest site; ( c ) Each 5′-terminal end is phosphorylated in order to ligate with 3′-terminal within the polypodna body, and ( d ) connecting chain is added to the 3′-terminal of the polypodna to allow polypodna to be connected to one another. The designed templates were amplified via the following steps. ( 1 ) The template ssODNs were circularized using T4 DNA ligase. ( 2 ) After annealing the primer (primer 1), the DNA template was amplified through rolling circle amplification technique using Phi29 polymerase. ( 3 ) Before enzyme digestion, the RCA product was treated with EDTA and folded. ( 4 ) Long single-stranded DNAs were digested using restriction enzyme. ( 5 ) The target sequences were purified by size chromatography. ( 6 ) The resultant DNAs self-assembled after annealing, and they formed a hydrogel.
    Figure Legend Snippet: Schematic diagram of the mass amplification of tripodna with adhesive 5′-ends. The template oligodeoxynucleotides (template 1) were designed to satisfy the following requirements: ( a ) the polypodna automatically forms by self-assembly; ( b ) each pod of the polypodna contains a 9 base long TspRI restriction digest site; ( c ) Each 5′-terminal end is phosphorylated in order to ligate with 3′-terminal within the polypodna body, and ( d ) connecting chain is added to the 3′-terminal of the polypodna to allow polypodna to be connected to one another. The designed templates were amplified via the following steps. ( 1 ) The template ssODNs were circularized using T4 DNA ligase. ( 2 ) After annealing the primer (primer 1), the DNA template was amplified through rolling circle amplification technique using Phi29 polymerase. ( 3 ) Before enzyme digestion, the RCA product was treated with EDTA and folded. ( 4 ) Long single-stranded DNAs were digested using restriction enzyme. ( 5 ) The target sequences were purified by size chromatography. ( 6 ) The resultant DNAs self-assembled after annealing, and they formed a hydrogel.

    Techniques Used: Amplification, Purification, Chromatography

    Related Articles

    Polymerase Chain Reaction:

    Article Title: The Role of the GABRA2 Polymorphism in Multiplex Alcohol Dependence Families With Minimal Comorbidity: Within-Family Association and Linkage Analyses
    Article Snippet: This polymorphism was analyzed by PCR amplification of a 151-base pair (bp) genomic fragment using the forward primer 5′-TGTGTATATTGGA GGGGGAAA-3′ and the reverse primer 5′-TGCAATAA TCCAATGATGCTG-3′. .. PCR products were digested with TspRI (New England Biolabs, Ispwich, MA). ..

    Article Title: Cutaneous leishmaniasis in French Guiana: revising epidemiology with PCR-RFLP
    Article Snippet: Then, the extracted DNA was amplified with RPOF2 (5′-AGAACATGGGCGGCC-3′) and RPOR2 (5′CGAGGGTCACGTTCTTG-3′) primers (Eurogentec) which target a 615-bp region of the RNA polymerase II gene. .. The PCR product was digested with TspRI or HgaI (New England Biolabs). .. The entire reaction mixture was then analyzed by electrophoresis in a 2% agarose gel containing ethidium bromide.

    Article Title: BRAF V600 mutations in Langerhans cell histiocytosis with a simple and unique assay
    Article Snippet: Restriction enzyme analysis TspRI (New England Biolabs) is a restriction enzyme that cuts at the sequence 5′-NNCASTGNN-3′ (3′-NNGTSACNN-5′); it can cut at the wild-type V600 BRAF codon in PCR amplicons across this region to give two fragments, but not sequences with V600E or V600D mutations. .. Digestion of PCR products with TspRI was performed at 65 °C in 1 × CutSmart Buffer (New England Biolabs). ..

    Article Title: Use of the Agilent 2100 Bioanalyzer for Rapid and Reproducible Molecular Typing of Streptococcus pneumoniae ▿
    Article Snippet: The PCR products (approximately 1.2 kb) were quantified using the DNA 7500 kit for the Agilent 2100 bioanalyzer (Agilent Technologies, Palo Alto, CA). .. Four hundred nanograms of each PCR product was digested separately with the restriction endonucleases DdeI, MseI, AluI, AfiIII, HaeIII, ApoI, TspRI, and TfiI (New England Biolabs, Ipswich, MA), according the manufacturer's instructions, in a total reaction mixture volume of 20 μl. .. Following digestion with DdeI, MseI, or AluI, the samples were heated to 65°C for 20 min, following digestion with the other enzymes to 80°C for 20 min, and then cooled on ice for 10 min to disrupt aggregates of DNA ( ) before 1 μl of the digestion product was analyzed using the Agilent 2100 bioanalyzer according to the manufacturer's protocol.

    Purification:

    Article Title: Efficient amplification of self-gelling polypod-like structured DNA by rolling circle amplification and enzymatic digestion
    Article Snippet: Polypodna production by restriction digestionThe highly viscous RCA product was incubated in 2 mM EDTA (Sigma-Aldrich, St. Louis, MO, USA) at 80 °C for 15 min to solubilize the product. .. After purification by size-exclusion chromatography, the resultant large molecular weight DNA was digested with 0.1 U/μL TspRI (New England Biolabs) in solution containing 50 mM potassium acetate, 20 mM Tris-acetate, 10 mM magnesium acetate, and 100 μg/ml BSA. .. The product was purified by size-exclusion chromatography to remove low molecular weight DNA waste.

    Size-exclusion Chromatography:

    Article Title: Efficient amplification of self-gelling polypod-like structured DNA by rolling circle amplification and enzymatic digestion
    Article Snippet: Polypodna production by restriction digestionThe highly viscous RCA product was incubated in 2 mM EDTA (Sigma-Aldrich, St. Louis, MO, USA) at 80 °C for 15 min to solubilize the product. .. After purification by size-exclusion chromatography, the resultant large molecular weight DNA was digested with 0.1 U/μL TspRI (New England Biolabs) in solution containing 50 mM potassium acetate, 20 mM Tris-acetate, 10 mM magnesium acetate, and 100 μg/ml BSA. .. The product was purified by size-exclusion chromatography to remove low molecular weight DNA waste.

    Molecular Weight:

    Article Title: Efficient amplification of self-gelling polypod-like structured DNA by rolling circle amplification and enzymatic digestion
    Article Snippet: Polypodna production by restriction digestionThe highly viscous RCA product was incubated in 2 mM EDTA (Sigma-Aldrich, St. Louis, MO, USA) at 80 °C for 15 min to solubilize the product. .. After purification by size-exclusion chromatography, the resultant large molecular weight DNA was digested with 0.1 U/μL TspRI (New England Biolabs) in solution containing 50 mM potassium acetate, 20 mM Tris-acetate, 10 mM magnesium acetate, and 100 μg/ml BSA. .. The product was purified by size-exclusion chromatography to remove low molecular weight DNA waste.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95
    New England Biolabs tspri
    CD1a immunohistochemistry FFPE tissue section from LCH patients stained with CD1a. ( a ) Two samples that were V600E mutation (+) after both primary <t>PCR</t> amplification and <t>TspRI</t> followed by additional PCR amplification. Four of five samples in this category demonstrated up to 70 % tumor content. These cases were easy to detect by primary PCR. ( b ) Examples of staining of samples that were BRAF mutation (-) after primary PCR, but mutation (+) after TspRI treatment. Seven of eight such samples contained
    Tspri, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tspri/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tspri - by Bioz Stars, 2021-06
    95/100 stars
      Buy from Supplier

    Image Search Results


    CD1a immunohistochemistry FFPE tissue section from LCH patients stained with CD1a. ( a ) Two samples that were V600E mutation (+) after both primary PCR amplification and TspRI followed by additional PCR amplification. Four of five samples in this category demonstrated up to 70 % tumor content. These cases were easy to detect by primary PCR. ( b ) Examples of staining of samples that were BRAF mutation (-) after primary PCR, but mutation (+) after TspRI treatment. Seven of eight such samples contained

    Journal: Diagnostic Pathology

    Article Title: BRAF V600 mutations in Langerhans cell histiocytosis with a simple and unique assay

    doi: 10.1186/s13000-016-0489-z

    Figure Lengend Snippet: CD1a immunohistochemistry FFPE tissue section from LCH patients stained with CD1a. ( a ) Two samples that were V600E mutation (+) after both primary PCR amplification and TspRI followed by additional PCR amplification. Four of five samples in this category demonstrated up to 70 % tumor content. These cases were easy to detect by primary PCR. ( b ) Examples of staining of samples that were BRAF mutation (-) after primary PCR, but mutation (+) after TspRI treatment. Seven of eight such samples contained

    Article Snippet: Digestion of PCR products with TspRI was performed at 65 °C in 1 × CutSmart Buffer (New England Biolabs).

    Techniques: Immunohistochemistry, Formalin-fixed Paraffin-Embedded, Staining, Mutagenesis, Polymerase Chain Reaction, Amplification

    The effect of TspRI restriction enzyme treatment on the BRAF sequence analysis of patient samples ( a ) Patient no. 21, representative sample with no V600E mutation, was judged mutation (-):GTG after primary PCR, and this conclusion was not changed by treatment with TspRI followed by secondary PCR; ( b ) patient no. 23 was scored as mutation (-):GTG after primary PCR, but mutation (+):GAG after treatment with TspRI; ( c ) the mutation status of patient no. 6 was difficult to judge from the primary PCR because of overlap between mutation (-):GTG and mutation (+):GAG sequences, however, after treatment with TspRI, the mutation (+):GAG sequence was dominant, indicating a clear mutation (+):GAG status; ( d ) patient no. 3 was initially judged mutation (-):GTG after primary PCR, but mutation (+):GAT status was apparent after treatment with TspRI

    Journal: Diagnostic Pathology

    Article Title: BRAF V600 mutations in Langerhans cell histiocytosis with a simple and unique assay

    doi: 10.1186/s13000-016-0489-z

    Figure Lengend Snippet: The effect of TspRI restriction enzyme treatment on the BRAF sequence analysis of patient samples ( a ) Patient no. 21, representative sample with no V600E mutation, was judged mutation (-):GTG after primary PCR, and this conclusion was not changed by treatment with TspRI followed by secondary PCR; ( b ) patient no. 23 was scored as mutation (-):GTG after primary PCR, but mutation (+):GAG after treatment with TspRI; ( c ) the mutation status of patient no. 6 was difficult to judge from the primary PCR because of overlap between mutation (-):GTG and mutation (+):GAG sequences, however, after treatment with TspRI, the mutation (+):GAG sequence was dominant, indicating a clear mutation (+):GAG status; ( d ) patient no. 3 was initially judged mutation (-):GTG after primary PCR, but mutation (+):GAT status was apparent after treatment with TspRI

    Article Snippet: Digestion of PCR products with TspRI was performed at 65 °C in 1 × CutSmart Buffer (New England Biolabs).

    Techniques: Sequencing, Mutagenesis, Polymerase Chain Reaction

    Optimization of TspRI digestion for the BRAF amplicons. 100 ng of BRAF PCR fragments amplified from normal DNA were digested with TspRI. Lane 1: No TspRI, 65 °C, 15 min. Lane 2: 5U TspRI, 65 °C, 5 min. Lane 3: 10U TspRI, 65 °C, 10 min. Lane 4: 20U TspRI, 65 °C, 15 min, leading to digestion of the majority of BRAF PCR fragments

    Journal: Diagnostic Pathology

    Article Title: BRAF V600 mutations in Langerhans cell histiocytosis with a simple and unique assay

    doi: 10.1186/s13000-016-0489-z

    Figure Lengend Snippet: Optimization of TspRI digestion for the BRAF amplicons. 100 ng of BRAF PCR fragments amplified from normal DNA were digested with TspRI. Lane 1: No TspRI, 65 °C, 15 min. Lane 2: 5U TspRI, 65 °C, 5 min. Lane 3: 10U TspRI, 65 °C, 10 min. Lane 4: 20U TspRI, 65 °C, 15 min, leading to digestion of the majority of BRAF PCR fragments

    Article Snippet: Digestion of PCR products with TspRI was performed at 65 °C in 1 × CutSmart Buffer (New England Biolabs).

    Techniques: Polymerase Chain Reaction, Amplification

    Schematic diagram of the mass amplification of tripodna with adhesive 5′-ends. The template oligodeoxynucleotides (template 1) were designed to satisfy the following requirements: ( a ) the polypodna automatically forms by self-assembly; ( b ) each pod of the polypodna contains a 9 base long TspRI restriction digest site; ( c ) Each 5′-terminal end is phosphorylated in order to ligate with 3′-terminal within the polypodna body, and ( d ) connecting chain is added to the 3′-terminal of the polypodna to allow polypodna to be connected to one another. The designed templates were amplified via the following steps. ( 1 ) The template ssODNs were circularized using T4 DNA ligase. ( 2 ) After annealing the primer (primer 1), the DNA template was amplified through rolling circle amplification technique using Phi29 polymerase. ( 3 ) Before enzyme digestion, the RCA product was treated with EDTA and folded. ( 4 ) Long single-stranded DNAs were digested using restriction enzyme. ( 5 ) The target sequences were purified by size chromatography. ( 6 ) The resultant DNAs self-assembled after annealing, and they formed a hydrogel.

    Journal: Scientific Reports

    Article Title: Efficient amplification of self-gelling polypod-like structured DNA by rolling circle amplification and enzymatic digestion

    doi: 10.1038/srep14979

    Figure Lengend Snippet: Schematic diagram of the mass amplification of tripodna with adhesive 5′-ends. The template oligodeoxynucleotides (template 1) were designed to satisfy the following requirements: ( a ) the polypodna automatically forms by self-assembly; ( b ) each pod of the polypodna contains a 9 base long TspRI restriction digest site; ( c ) Each 5′-terminal end is phosphorylated in order to ligate with 3′-terminal within the polypodna body, and ( d ) connecting chain is added to the 3′-terminal of the polypodna to allow polypodna to be connected to one another. The designed templates were amplified via the following steps. ( 1 ) The template ssODNs were circularized using T4 DNA ligase. ( 2 ) After annealing the primer (primer 1), the DNA template was amplified through rolling circle amplification technique using Phi29 polymerase. ( 3 ) Before enzyme digestion, the RCA product was treated with EDTA and folded. ( 4 ) Long single-stranded DNAs were digested using restriction enzyme. ( 5 ) The target sequences were purified by size chromatography. ( 6 ) The resultant DNAs self-assembled after annealing, and they formed a hydrogel.

    Article Snippet: After purification by size-exclusion chromatography, the resultant large molecular weight DNA was digested with 0.1 U/μL TspRI (New England Biolabs) in solution containing 50 mM potassium acetate, 20 mM Tris-acetate, 10 mM magnesium acetate, and 100 μg/ml BSA.

    Techniques: Amplification, Purification, Chromatography