cac8i  (New England Biolabs)


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    Name:
    Cac8I
    Description:
    Cac8I 500 units
    Catalog Number:
    R0579L
    Price:
    302
    Category:
    Restriction Enzymes
    Size:
    500 units
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    Structured Review

    New England Biolabs cac8i
    Cac8I
    Cac8I 500 units
    https://www.bioz.com/result/cac8i/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cac8i - by Bioz Stars, 2021-06
    93/100 stars

    Images

    1) Product Images from "An Activin Receptor IA/Activin-Like Kinase-2 (R206H) Mutation in Fibrodysplasia Ossificans Progressiva"

    Article Title: An Activin Receptor IA/Activin-Like Kinase-2 (R206H) Mutation in Fibrodysplasia Ossificans Progressiva

    Journal: Case Reports in Genetics

    doi: 10.1155/2013/260371

    Genotyping in top panel: PCR amplification products were resolved by 3% agarose gel electrophoresis (C: control, M: mother, F: father, and P: FOP patient). As shown on the left, similar 350 bp products were amplified in controls, relatives, and the FOP case; however, after Cac8I digestion, a 253 bp fragment was observed exclusively in the FOP patient (marked with arrow). Bottom panel: a chromatogram showing the typical mutation at position 617, which causes an amino acid substitution of histidine for arginine at position 206 in the GS domain of ACVR1/ALK2.
    Figure Legend Snippet: Genotyping in top panel: PCR amplification products were resolved by 3% agarose gel electrophoresis (C: control, M: mother, F: father, and P: FOP patient). As shown on the left, similar 350 bp products were amplified in controls, relatives, and the FOP case; however, after Cac8I digestion, a 253 bp fragment was observed exclusively in the FOP patient (marked with arrow). Bottom panel: a chromatogram showing the typical mutation at position 617, which causes an amino acid substitution of histidine for arginine at position 206 in the GS domain of ACVR1/ALK2.

    Techniques Used: Polymerase Chain Reaction, Amplification, Agarose Gel Electrophoresis, Mutagenesis

    2) Product Images from "ACVR1 Gene Mutation in Sporadic Korean Patients with Fibrodysplasia Ossificans Progressiva"

    Article Title: ACVR1 Gene Mutation in Sporadic Korean Patients with Fibrodysplasia Ossificans Progressiva

    Journal: Journal of Korean Medical Science

    doi: 10.3346/jkms.2009.24.3.433

    Mutation analysis of the ACVR1 in FOP patients with definite clinical manifestations. ( A ) Direct sequencing of the PCR products of the ACVR1 showed the presence of the c.617G > A mutation. R=adenine or guanine, ( B ) Restriction endonuclease digestion of the PCR product (350 bp). The G allele (control) was digested by Cac8I to produce three bands, whereas the A allele appeared as two bands. Because FOP patients were heterozygous for this mutation, the 139 bp and 114 bp bands were also presented. The PCR product not digested with HphI corresponds to the G allele (control) in contrast to digested products corresponding to the A allele (FOP).
    Figure Legend Snippet: Mutation analysis of the ACVR1 in FOP patients with definite clinical manifestations. ( A ) Direct sequencing of the PCR products of the ACVR1 showed the presence of the c.617G > A mutation. R=adenine or guanine, ( B ) Restriction endonuclease digestion of the PCR product (350 bp). The G allele (control) was digested by Cac8I to produce three bands, whereas the A allele appeared as two bands. Because FOP patients were heterozygous for this mutation, the 139 bp and 114 bp bands were also presented. The PCR product not digested with HphI corresponds to the G allele (control) in contrast to digested products corresponding to the A allele (FOP).

    Techniques Used: Mutagenesis, Sequencing, Polymerase Chain Reaction

    3) Product Images from "P4HA1 mutations cause a unique congenital disorder of connective tissue involving tendon, bone, muscle and the eye"

    Article Title: P4HA1 mutations cause a unique congenital disorder of connective tissue involving tendon, bone, muscle and the eye

    Journal: Human Molecular Genetics

    doi: 10.1093/hmg/ddx110

    ( A ) Total C-P4H activity was reduced in cultured fibroblasts from P1 and P2 compared to age-matched controls ( C1 and C2 ). Activities in both carrier father ( F ) and carrier mother ( M ) were within normal range, although it was at the lower end of the range in the carrier mother. ( B ) Western blot analysis with α-tubulin loading control. C-P4H α (I) protein is reduced in patients-derived fibroblasts compared to age-matched controls ( C1 and C2 ). There is no up-regulation of C-P4H α (II) protein in cultured fibroblasts from P1 , P2 and their parents compared to controls. ( C ) Ratio of exon 9 versus exon 10 splice form in muscle tissue and cultured dermal fibroblasts from individuals of different ages as determined by RT-PCR followed with Cac8I (specific for exon 9) or BlpI (specific for exon 10) digestion. The exon 9 splice form is the major form expressed in muscle tissue, the average ratio of exon 9/exon 10 is 4.22 (SEM = 0.59) in younger individuals (
    Figure Legend Snippet: ( A ) Total C-P4H activity was reduced in cultured fibroblasts from P1 and P2 compared to age-matched controls ( C1 and C2 ). Activities in both carrier father ( F ) and carrier mother ( M ) were within normal range, although it was at the lower end of the range in the carrier mother. ( B ) Western blot analysis with α-tubulin loading control. C-P4H α (I) protein is reduced in patients-derived fibroblasts compared to age-matched controls ( C1 and C2 ). There is no up-regulation of C-P4H α (II) protein in cultured fibroblasts from P1 , P2 and their parents compared to controls. ( C ) Ratio of exon 9 versus exon 10 splice form in muscle tissue and cultured dermal fibroblasts from individuals of different ages as determined by RT-PCR followed with Cac8I (specific for exon 9) or BlpI (specific for exon 10) digestion. The exon 9 splice form is the major form expressed in muscle tissue, the average ratio of exon 9/exon 10 is 4.22 (SEM = 0.59) in younger individuals (

    Techniques Used: Activity Assay, Cell Culture, Western Blot, Derivative Assay, Reverse Transcription Polymerase Chain Reaction

    4) Product Images from "ACVR1 Gene Mutation in Sporadic Korean Patients with Fibrodysplasia Ossificans Progressiva"

    Article Title: ACVR1 Gene Mutation in Sporadic Korean Patients with Fibrodysplasia Ossificans Progressiva

    Journal: Journal of Korean Medical Science

    doi: 10.3346/jkms.2009.24.3.433

    Mutation analysis of the ACVR1 in FOP patients with definite clinical manifestations. ( A ) Direct sequencing of the PCR products of the ACVR1 showed the presence of the c.617G > A mutation. R=adenine or guanine, ( B ) Restriction endonuclease digestion of the PCR product (350 bp). The G allele (control) was digested by Cac8I to produce three bands, whereas the A allele appeared as two bands. Because FOP patients were heterozygous for this mutation, the 139 bp and 114 bp bands were also presented. The PCR product not digested with HphI corresponds to the G allele (control) in contrast to digested products corresponding to the A allele (FOP).
    Figure Legend Snippet: Mutation analysis of the ACVR1 in FOP patients with definite clinical manifestations. ( A ) Direct sequencing of the PCR products of the ACVR1 showed the presence of the c.617G > A mutation. R=adenine or guanine, ( B ) Restriction endonuclease digestion of the PCR product (350 bp). The G allele (control) was digested by Cac8I to produce three bands, whereas the A allele appeared as two bands. Because FOP patients were heterozygous for this mutation, the 139 bp and 114 bp bands were also presented. The PCR product not digested with HphI corresponds to the G allele (control) in contrast to digested products corresponding to the A allele (FOP).

    Techniques Used: Mutagenesis, Sequencing, Polymerase Chain Reaction

    5) Product Images from "P4HA1 mutations cause a unique congenital disorder of connective tissue involving tendon, bone, muscle and the eye"

    Article Title: P4HA1 mutations cause a unique congenital disorder of connective tissue involving tendon, bone, muscle and the eye

    Journal: Human Molecular Genetics

    doi: 10.1093/hmg/ddx110

    ( A ) Total C-P4H activity was reduced in cultured fibroblasts from P1 and P2 compared to age-matched controls ( C1 and C2 ). Activities in both carrier father ( F ) and carrier mother ( M ) were within normal range, although it was at the lower end of the range in the carrier mother. ( B ) Western blot analysis with α-tubulin loading control. C-P4H α (I) protein is reduced in patients-derived fibroblasts compared to age-matched controls ( C1 and C2 ). There is no up-regulation of C-P4H α (II) protein in cultured fibroblasts from P1 , P2 and their parents compared to controls. ( C ) Ratio of exon 9 versus exon 10 splice form in muscle tissue and cultured dermal fibroblasts from individuals of different ages as determined by RT-PCR followed with Cac8I (specific for exon 9) or BlpI (specific for exon 10) digestion. The exon 9 splice form is the major form expressed in muscle tissue, the average ratio of exon 9/exon 10 is 4.22 (SEM = 0.59) in younger individuals (
    Figure Legend Snippet: ( A ) Total C-P4H activity was reduced in cultured fibroblasts from P1 and P2 compared to age-matched controls ( C1 and C2 ). Activities in both carrier father ( F ) and carrier mother ( M ) were within normal range, although it was at the lower end of the range in the carrier mother. ( B ) Western blot analysis with α-tubulin loading control. C-P4H α (I) protein is reduced in patients-derived fibroblasts compared to age-matched controls ( C1 and C2 ). There is no up-regulation of C-P4H α (II) protein in cultured fibroblasts from P1 , P2 and their parents compared to controls. ( C ) Ratio of exon 9 versus exon 10 splice form in muscle tissue and cultured dermal fibroblasts from individuals of different ages as determined by RT-PCR followed with Cac8I (specific for exon 9) or BlpI (specific for exon 10) digestion. The exon 9 splice form is the major form expressed in muscle tissue, the average ratio of exon 9/exon 10 is 4.22 (SEM = 0.59) in younger individuals (

    Techniques Used: Activity Assay, Cell Culture, Western Blot, Derivative Assay, Reverse Transcription Polymerase Chain Reaction

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Association of a Cac8I polymorphism in the IGF1 gene with growth traits in Indian goats
    Article Snippet: NEBcutter tool was used for designing a novel PCR-RFLP for the SNP. .. PCR-RFLP protocol was formulated using restriction enzymes CaC8I (NEB) for simple and quick genotyping the samples. .. The PCR-amplified DNA fragments of the IGF1 gene were digested at 37 °C for two hours with five units of restriction enzyme CaC8I (NEB).

    Article Title: An Activin Receptor IA/Activin-Like Kinase-2 (R206H) Mutation in Fibrodysplasia Ossificans Progressiva
    Article Snippet: Total genomic DNA was used as a template for PCR amplification (Select Cycler, Bio Products) with the following exon flanking primers: exon 4 forward 5′-CCA GTC CTT CTT CCT TCT TCC-3′ and reverse 5′-AGC AGA TTT TCC AAG TTC CAT C-3′; exon 6 forward 5′-GAC ATT TAC TGT GTA GGT CGC-3′ and reverse 5′-AGA GAT GCA ACT CAC CTA ACC-3′ and previously reported PCR conditions [ ]. .. After amplification, the PCR products were digested with Cac8I and HphI restriction enzymes (New England Biolabs) for 1 h at 37°C and then submitted to 3% agarose gel electrophoresis. .. The undigested PCR products were prepared for sequencing using ultraclean PCR columns, in which a silica membrane assembly binds the DNA and allows the removal of primers, nucleotides, and enzymes.

    Article Title: ACVR1 Gene Mutation in Sporadic Korean Patients with Fibrodysplasia Ossificans Progressiva
    Article Snippet: The c.617G > A ACVR1 mutation eliminates a Cac8I site and forms a new HphI site. .. The PCR product of 350 bp from the G allele (control-parents) was digested by Cac8I and produced three bands (139, 114, and 97 bp), whereas the A allele (FOP patients) appeared as two bands (253 and 97 bp). .. For HphI , PCR products of controls were not digested (one band) whereas bands of 228 and 122 bp, corresponding to the A allele, were detected for all FOP patients ( ).

    Amplification:

    Article Title: An Activin Receptor IA/Activin-Like Kinase-2 (R206H) Mutation in Fibrodysplasia Ossificans Progressiva
    Article Snippet: Total genomic DNA was used as a template for PCR amplification (Select Cycler, Bio Products) with the following exon flanking primers: exon 4 forward 5′-CCA GTC CTT CTT CCT TCT TCC-3′ and reverse 5′-AGC AGA TTT TCC AAG TTC CAT C-3′; exon 6 forward 5′-GAC ATT TAC TGT GTA GGT CGC-3′ and reverse 5′-AGA GAT GCA ACT CAC CTA ACC-3′ and previously reported PCR conditions [ ]. .. After amplification, the PCR products were digested with Cac8I and HphI restriction enzymes (New England Biolabs) for 1 h at 37°C and then submitted to 3% agarose gel electrophoresis. .. The undigested PCR products were prepared for sequencing using ultraclean PCR columns, in which a silica membrane assembly binds the DNA and allows the removal of primers, nucleotides, and enzymes.

    Agarose Gel Electrophoresis:

    Article Title: An Activin Receptor IA/Activin-Like Kinase-2 (R206H) Mutation in Fibrodysplasia Ossificans Progressiva
    Article Snippet: Total genomic DNA was used as a template for PCR amplification (Select Cycler, Bio Products) with the following exon flanking primers: exon 4 forward 5′-CCA GTC CTT CTT CCT TCT TCC-3′ and reverse 5′-AGC AGA TTT TCC AAG TTC CAT C-3′; exon 6 forward 5′-GAC ATT TAC TGT GTA GGT CGC-3′ and reverse 5′-AGA GAT GCA ACT CAC CTA ACC-3′ and previously reported PCR conditions [ ]. .. After amplification, the PCR products were digested with Cac8I and HphI restriction enzymes (New England Biolabs) for 1 h at 37°C and then submitted to 3% agarose gel electrophoresis. .. The undigested PCR products were prepared for sequencing using ultraclean PCR columns, in which a silica membrane assembly binds the DNA and allows the removal of primers, nucleotides, and enzymes.

    Activity Assay:

    Article Title: Mitotic chromosomes are chromatin networks without a mechanically contiguous protein scaffold
    Article Snippet: .. The large difference in activity of Cac 8I and Alu I is somewhat surprising, because the recognition sequences differ by only 2 bp in length, a small fraction of the 20–40 bp between nucleosomes. ..

    Article Title: Mitotic chromosomes are chromatin networks without a mechanically contiguous protein scaffold
    Article Snippet: .. I digestion is available at Experiments with Cac 8I were done to examine the effect of size of the recognition sequence length on enzyme activity. .. Cac 8I's recognition sequence has the same statistical frequency on random-sequence DNA (1/44 = 1/256) as those of Alu I and Hae III but is spread over a six-base footprint.

    other:

    Article Title: Mitotic chromosomes are chromatin networks without a mechanically contiguous protein scaffold
    Article Snippet: We therefore estimate that Cac 8I can cut metaphase chromatin about once every 25 kb.

    Produced:

    Article Title: ACVR1 Gene Mutation in Sporadic Korean Patients with Fibrodysplasia Ossificans Progressiva
    Article Snippet: The c.617G > A ACVR1 mutation eliminates a Cac8I site and forms a new HphI site. .. The PCR product of 350 bp from the G allele (control-parents) was digested by Cac8I and produced three bands (139, 114, and 97 bp), whereas the A allele (FOP patients) appeared as two bands (253 and 97 bp). .. For HphI , PCR products of controls were not digested (one band) whereas bands of 228 and 122 bp, corresponding to the A allele, were detected for all FOP patients ( ).

    Sequencing:

    Article Title: Mitotic chromosomes are chromatin networks without a mechanically contiguous protein scaffold
    Article Snippet: .. I digestion is available at Experiments with Cac 8I were done to examine the effect of size of the recognition sequence length on enzyme activity. .. Cac 8I's recognition sequence has the same statistical frequency on random-sequence DNA (1/44 = 1/256) as those of Alu I and Hae III but is spread over a six-base footprint.

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    New England Biolabs cac8 i
    Pedigree of the family and the results of the restriction analysis. Since Q353X results in a loss of a restriction site for Cac8 I, digestion of exon 6 with <t>Cac8</t> I results in an undigested 102 band in the patient, in one of his sisters and mother. In contrast,
    Cac8 I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cac8 i/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    cac8 i - by Bioz Stars, 2021-06
    93/100 stars
      Buy from Supplier

    Image Search Results


    Pedigree of the family and the results of the restriction analysis. Since Q353X results in a loss of a restriction site for Cac8 I, digestion of exon 6 with Cac8 I results in an undigested 102 band in the patient, in one of his sisters and mother. In contrast,

    Journal: Human genetics

    Article Title: LG2 Agrin Mutation Causing Severe Congenital Myasthenic Syndrome Mimics Functional Characteristics of Non-neural (z−) Agrin

    doi: 10.1007/s00439-011-1132-4

    Figure Lengend Snippet: Pedigree of the family and the results of the restriction analysis. Since Q353X results in a loss of a restriction site for Cac8 I, digestion of exon 6 with Cac8 I results in an undigested 102 band in the patient, in one of his sisters and mother. In contrast,

    Article Snippet: Digestion of the Agrin Q353X and V1727F amplicons was performed using Cac8 I and SexA I restriction enzymes respectively (New England BioLabs, Ipswich, MA, USA) according to the manufacturer's protocol.

    Techniques:

    Genotyping in top panel: PCR amplification products were resolved by 3% agarose gel electrophoresis (C: control, M: mother, F: father, and P: FOP patient). As shown on the left, similar 350 bp products were amplified in controls, relatives, and the FOP case; however, after Cac8I digestion, a 253 bp fragment was observed exclusively in the FOP patient (marked with arrow). Bottom panel: a chromatogram showing the typical mutation at position 617, which causes an amino acid substitution of histidine for arginine at position 206 in the GS domain of ACVR1/ALK2.

    Journal: Case Reports in Genetics

    Article Title: An Activin Receptor IA/Activin-Like Kinase-2 (R206H) Mutation in Fibrodysplasia Ossificans Progressiva

    doi: 10.1155/2013/260371

    Figure Lengend Snippet: Genotyping in top panel: PCR amplification products were resolved by 3% agarose gel electrophoresis (C: control, M: mother, F: father, and P: FOP patient). As shown on the left, similar 350 bp products were amplified in controls, relatives, and the FOP case; however, after Cac8I digestion, a 253 bp fragment was observed exclusively in the FOP patient (marked with arrow). Bottom panel: a chromatogram showing the typical mutation at position 617, which causes an amino acid substitution of histidine for arginine at position 206 in the GS domain of ACVR1/ALK2.

    Article Snippet: After amplification, the PCR products were digested with Cac8I and HphI restriction enzymes (New England Biolabs) for 1 h at 37°C and then submitted to 3% agarose gel electrophoresis.

    Techniques: Polymerase Chain Reaction, Amplification, Agarose Gel Electrophoresis, Mutagenesis

    Sequence map of GG and AG genotypes indicating A224G , with Cac8I restriction site.

    Journal: Journal of Genetic Engineering & Biotechnology

    Article Title: Association of a Cac8I polymorphism in the IGF1 gene with growth traits in Indian goats

    doi: 10.1016/j.jgeb.2017.04.002

    Figure Lengend Snippet: Sequence map of GG and AG genotypes indicating A224G , with Cac8I restriction site.

    Article Snippet: PCR-RFLP protocol was formulated using restriction enzymes CaC8I (NEB) for simple and quick genotyping the samples.

    Techniques: Sequencing

    Pattern of Cac8I digestion of A224G of 294 bp fragment of IGF1 with AG and GG genotypes. Lane 1: 294 bp PCR product, Lanes 2, 4 5: AG genotype, Lane 3: GG genotype, Lane 6: 50 bp marker.

    Journal: Journal of Genetic Engineering & Biotechnology

    Article Title: Association of a Cac8I polymorphism in the IGF1 gene with growth traits in Indian goats

    doi: 10.1016/j.jgeb.2017.04.002

    Figure Lengend Snippet: Pattern of Cac8I digestion of A224G of 294 bp fragment of IGF1 with AG and GG genotypes. Lane 1: 294 bp PCR product, Lanes 2, 4 5: AG genotype, Lane 3: GG genotype, Lane 6: 50 bp marker.

    Article Snippet: PCR-RFLP protocol was formulated using restriction enzymes CaC8I (NEB) for simple and quick genotyping the samples.

    Techniques: Polymerase Chain Reaction, Marker

    REs with increasing specificity show decreasing effects on chromosome elastic response. Force data are before, during, and after 350-sec exposures to various REs; force is normalized to units of initial applied force, which ranged between 0.2 and 0.8 nN in the five separate experiments shown. Alu I AG↓CT (black) relaxes the force in ≈30 sec; Cac 8I GCN↓NGC (red) only partially reduces the force. Hin cII GT(T/C)↓(A/G)AC (blue) and Dra I TTT↓AAA (green) induce an increase in force during spraying, with a return to the original force when spraying stops (≈600 sec), similar to spraying with reaction buffer and no enzyme (violet). These results indicate that chromatin–chromatin crosslinks occur roughly every 15 kb (see text). A video of Alu .

    Journal: Proceedings of the National Academy of Sciences of the United States of America

    Article Title: Mitotic chromosomes are chromatin networks without a mechanically contiguous protein scaffold

    doi: 10.1073/pnas.232442599

    Figure Lengend Snippet: REs with increasing specificity show decreasing effects on chromosome elastic response. Force data are before, during, and after 350-sec exposures to various REs; force is normalized to units of initial applied force, which ranged between 0.2 and 0.8 nN in the five separate experiments shown. Alu I AG↓CT (black) relaxes the force in ≈30 sec; Cac 8I GCN↓NGC (red) only partially reduces the force. Hin cII GT(T/C)↓(A/G)AC (blue) and Dra I TTT↓AAA (green) induce an increase in force during spraying, with a return to the original force when spraying stops (≈600 sec), similar to spraying with reaction buffer and no enzyme (violet). These results indicate that chromatin–chromatin crosslinks occur roughly every 15 kb (see text). A video of Alu .

    Article Snippet: I digestion is available at Experiments with Cac 8I were done to examine the effect of size of the recognition sequence length on enzyme activity.

    Techniques: Size-exclusion Chromatography