Journal: PLoS ONE
Article Title: The T309G MDM2 Gene Polymorphism Is a Novel Risk Factor for Proliferative Vitreoretinopathy
Figure Lengend Snippet: PCR-RFLP to determine MDM 2 SNP309 polymorphism. MDM 2 SNP309 T allele is not cleaved by MspA1I endonuclease and generates a single fragment of 155 bp. The MDM 2 SNP309 G allele is cleaved by MspA1I and generates two small fragments of 101 and 54 bp. The MDM 2 SNP309 heterozygote displays three fragments of 155, 101 and 54 bp.
Article Snippet: MDM2 intron 1 was amplified within a 155 pb DNA fragment that was digested with the restriction endonuclease MspA1I (New England Biolabs, Inc.).
Techniques: Polymerase Chain Reaction