mspa1i  (New England Biolabs)


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    Name:
    MspA1I
    Description:
    MspA1I 2 500 units
    Catalog Number:
    r0577l
    Price:
    290
    Category:
    Restriction Enzymes
    Size:
    2 500 units
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    Structured Review

    New England Biolabs mspa1i
    MspA1I
    MspA1I 2 500 units
    https://www.bioz.com/result/mspa1i/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    mspa1i - by Bioz Stars, 2021-03
    93/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: A functional polymorphism T309G in MDM2 gene promoter, intensified by Helicobacter pylori lipopolysaccharide, is associated with both an increased susceptibility and poor prognosis of gastric carcinoma in Chinese patients
    Article Snippet: In brief, the primer sequences were 5P-CGCGGGAGTTCAGGGTAAAG-3P (forward) and 5P-AGCTGGAGACAAGTCAGGACTTAAC-3P (reverse), which generated the 237-bp fragment. .. The PCR product was then digested by MSPA1I (New England Biolabs, Bevely, MA, USA). .. The wild-type (SNP309T) allele produces a single 237-bp fragment and the variant (SNP309G) allele produced two fragments of 189- and 48 bp.

    Article Title: Hypomorphic sialidase expression decreases serum cholesterol by downregulation of VLDL production in mice
    Article Snippet: The following primers were used for the PCR: 5′ ATC CCT GTC CAG GAA CTG GT 3′ and 5′ CTT AAG GGC ATT GGG GTC AT 3′, synthesized by Mobix facility at McMaster University. .. PCR (40 cycles) was performed with denaturing temperature at 94°C for 2 min, annealing temperature at 60°C for 30 s, and elongation temperature at 72°C for 30 s. PCR products were digested with MspA1I (New England BioLab), which serves as a genetic diagnostic as it only cleaves the PCR product carrying the B6.SM mutation. .. Mice were housed in microisolator cages in a room with a 12 h light and dark cycle and given unlimited access to food and water.

    Article Title: Highly variable response to cytotoxic chemotherapy in carcinoma-associated fibroblasts (CAFs) from lung and breast
    Article Snippet: Click here for file Additional file 2 PCR/RFLP analysis of the Mdm2-309T/G polymorphism. .. Representative PCR/RFLP patterns for the different Mdm2 genotypes: T/T homozygous uncleaved by MspA1I (lanes 1, 4, 5); heterozygous cleaved by MspA1I yielding two bands (lanes 3, 6, 7); G/G homozygous completely cleaved by MspA1I (lanes 2, 8, 9). .. Click here for file Additional file 3 Effect of neoadjuvant chemotherapy on stromal and tumor compartment in vivo .

    Article Title: Transforming growth factor ? 869C/T and interleukin 6 -174G/C polymorphisms relate to the severity and progression of bone-erosive damage detected by ultrasound in rheumatoid arthritis
    Article Snippet: The forward and reverse primers were 5'-TTCCCTCGAGGCCCTCCTA-3' and 5'-GCCGCAGCTTGGACAGGATC-3', and the PCR amplification protocol was composed of 35 cycles comprising three steps each: 75 seconds at 96°C, 75 seconds at 62°C and 75 seconds at 73°C. .. PCR products were digested with Msp A1I (New England BioLabs, Ipswich, MA, US) and run on a 3% ethidium bromide-stained agarose gel. .. The -174G/C IL-6 promoter SNP was analyzed by PCR amplification and digestion with a site-specific restriction enzyme using previously reported methods [ ].

    Article Title: Novel CACNA1S mutation causes autosomal dominant hypokalemic periodic paralysis in a Chinese family
    Article Snippet: We PCR-amplified exon 11 containing the Arg528Gly mutation from all members of the family as well as 200 unrelated healthy Chinese individuals of Han nationality. .. The 364-bp PCR product was digested with 1 U of Msp A1 I (New England Biolabs) at 37°C overnight. ..

    Diagnostic Assay:

    Article Title: Hypomorphic sialidase expression decreases serum cholesterol by downregulation of VLDL production in mice
    Article Snippet: The following primers were used for the PCR: 5′ ATC CCT GTC CAG GAA CTG GT 3′ and 5′ CTT AAG GGC ATT GGG GTC AT 3′, synthesized by Mobix facility at McMaster University. .. PCR (40 cycles) was performed with denaturing temperature at 94°C for 2 min, annealing temperature at 60°C for 30 s, and elongation temperature at 72°C for 30 s. PCR products were digested with MspA1I (New England BioLab), which serves as a genetic diagnostic as it only cleaves the PCR product carrying the B6.SM mutation. .. Mice were housed in microisolator cages in a room with a 12 h light and dark cycle and given unlimited access to food and water.

    Mutagenesis:

    Article Title: Hypomorphic sialidase expression decreases serum cholesterol by downregulation of VLDL production in mice
    Article Snippet: The following primers were used for the PCR: 5′ ATC CCT GTC CAG GAA CTG GT 3′ and 5′ CTT AAG GGC ATT GGG GTC AT 3′, synthesized by Mobix facility at McMaster University. .. PCR (40 cycles) was performed with denaturing temperature at 94°C for 2 min, annealing temperature at 60°C for 30 s, and elongation temperature at 72°C for 30 s. PCR products were digested with MspA1I (New England BioLab), which serves as a genetic diagnostic as it only cleaves the PCR product carrying the B6.SM mutation. .. Mice were housed in microisolator cages in a room with a 12 h light and dark cycle and given unlimited access to food and water.

    Agarose Gel Electrophoresis:

    Article Title: Transforming growth factor ? 869C/T and interleukin 6 -174G/C polymorphisms relate to the severity and progression of bone-erosive damage detected by ultrasound in rheumatoid arthritis
    Article Snippet: The forward and reverse primers were 5'-TTCCCTCGAGGCCCTCCTA-3' and 5'-GCCGCAGCTTGGACAGGATC-3', and the PCR amplification protocol was composed of 35 cycles comprising three steps each: 75 seconds at 96°C, 75 seconds at 62°C and 75 seconds at 73°C. .. PCR products were digested with Msp A1I (New England BioLabs, Ipswich, MA, US) and run on a 3% ethidium bromide-stained agarose gel. .. The -174G/C IL-6 promoter SNP was analyzed by PCR amplification and digestion with a site-specific restriction enzyme using previously reported methods [ ].

    Article Title: Frequency of TNFR1 36 A/G gene polymorphism in azoospermic infertile men: A case-control study
    Article Snippet: Digestion by MSPA1I enzyme After PCR reactions, the final analysis was performed by PCR-RFLP method with the help of restriction MSPA1I enzyme for all participants. .. After optimizing the enzymatic digestion conditions, samples were cut and then were taken on 3% agarose gel by enzyme MSPA1I (New England Biolabs (Neb) Co. ..

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  • 93
    New England Biolabs enzyme mspa1i
    Image of products digested by a <t>MSPA1I</t> enzyme on an agarose gel.
    Enzyme Mspa1i, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/enzyme mspa1i/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    enzyme mspa1i - by Bioz Stars, 2021-03
    93/100 stars
      Buy from Supplier

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    Image of products digested by a MSPA1I enzyme on an agarose gel.

    Journal: International Journal of Reproductive Biomedicine

    Article Title: Frequency of TNFR1 36 A/G gene polymorphism in azoospermic infertile men: A case-control study

    doi:

    Figure Lengend Snippet: Image of products digested by a MSPA1I enzyme on an agarose gel.

    Article Snippet: After optimizing the enzymatic digestion conditions, samples were cut and then were taken on 3% agarose gel by enzyme MSPA1I (New England Biolabs (Neb) Co.

    Techniques: Agarose Gel Electrophoresis