bsrdi  (New England Biolabs)


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    Name:
    BsrDI
    Description:
    BsrDI 1 000 units
    Catalog Number:
    R0574L
    Price:
    269
    Category:
    Restriction Enzymes
    Size:
    1 000 units
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    Structured Review

    New England Biolabs bsrdi
    BsrDI
    BsrDI 1 000 units
    https://www.bioz.com/result/bsrdi/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bsrdi - by Bioz Stars, 2021-04
    95/100 stars

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    1) Product Images from "Hepatitis B virus cccDNA is formed through distinct repair processes of each strand"

    Article Title: Hepatitis B virus cccDNA is formed through distinct repair processes of each strand

    Journal: Nature Communications

    doi: 10.1038/s41467-021-21850-9

    Repair of HBV lesions on plus- and minus-strands require different sets of protein factors. a Characteristics of HBV rcDNA structure. b Schematic representation of the generation of recombinant HBV rcDNA (RrcDNA) substrates that contain all lesions (RrcDNA) or only lesions on the plus- (psl-RrcDNA) or minus-strands (msl-RrcDNA). HBV plasmids contains a plasmid backbone (gray) and either the minus strand sequence (black) or the plus-strand sequence (blue). BsrDI cleavage was used to monitor the generation of various RrcDNA substrates (BsrDI restriction sites are indicated by magenta arrows); green line, biotinylated flap; B, biotin; red line, RNA primer. Note that M1 and M2 are two oligos released from the minus-strands of RrcDNA precursor and RrcDNA after BsrDI digestion, whereas P1 and P2 are two oligos released from the plus-strands after BsrDI digestion. c Annealing products of psl-RrcDNA and msl-RrcDNA precursors were monitored by Sybr Safe staining. d Generation of various RrcDNA precursors was analyzed by formation of M2 and P2 oligos from the corresponding M1 and P1 oligos after BsrDI digestion and urea-PAGE gel electrophoresis followed by Sybr Gold staining. Please note that psl-RcDNA and msl-RrcDNA only contain annealed oligos on the plus-strand and minus strand, respectively. Almost complete conversion from P1 to P2 (psl-RcDNA, lanes 5–6), and M1 to M2 (msl-RcDNA, lanes 3–4) was observed. e All five human protein factors are required for repair of the lesions on the plus-strand. Psl-RrcDNA was mixed with combinations of purified proteins, and cccDNA formation was detected on agarose gels containing ethidium bromide (EtBr). Omission of factors is indicated by “−”. f FEN-1 and LIG1 are necessary and sufficient for repair of lesions on the minus strand. Omission of factors is indicated by “−”. The percentage of cccDNA formed (% repaired) was calculated by dividing the intensity of the ccc band by the sum of the intensities of the RrcDNA, linear RrcDNA and cccDNA bands. The absolute values are displayed above each lane number. Rrc, RrcDNA; rL, recombinant linear RrcDNA; ccc, cccDNA. All experiments were repeated twice with the same results. Source data are provided as a Source Data file.
    Figure Legend Snippet: Repair of HBV lesions on plus- and minus-strands require different sets of protein factors. a Characteristics of HBV rcDNA structure. b Schematic representation of the generation of recombinant HBV rcDNA (RrcDNA) substrates that contain all lesions (RrcDNA) or only lesions on the plus- (psl-RrcDNA) or minus-strands (msl-RrcDNA). HBV plasmids contains a plasmid backbone (gray) and either the minus strand sequence (black) or the plus-strand sequence (blue). BsrDI cleavage was used to monitor the generation of various RrcDNA substrates (BsrDI restriction sites are indicated by magenta arrows); green line, biotinylated flap; B, biotin; red line, RNA primer. Note that M1 and M2 are two oligos released from the minus-strands of RrcDNA precursor and RrcDNA after BsrDI digestion, whereas P1 and P2 are two oligos released from the plus-strands after BsrDI digestion. c Annealing products of psl-RrcDNA and msl-RrcDNA precursors were monitored by Sybr Safe staining. d Generation of various RrcDNA precursors was analyzed by formation of M2 and P2 oligos from the corresponding M1 and P1 oligos after BsrDI digestion and urea-PAGE gel electrophoresis followed by Sybr Gold staining. Please note that psl-RcDNA and msl-RrcDNA only contain annealed oligos on the plus-strand and minus strand, respectively. Almost complete conversion from P1 to P2 (psl-RcDNA, lanes 5–6), and M1 to M2 (msl-RcDNA, lanes 3–4) was observed. e All five human protein factors are required for repair of the lesions on the plus-strand. Psl-RrcDNA was mixed with combinations of purified proteins, and cccDNA formation was detected on agarose gels containing ethidium bromide (EtBr). Omission of factors is indicated by “−”. f FEN-1 and LIG1 are necessary and sufficient for repair of lesions on the minus strand. Omission of factors is indicated by “−”. The percentage of cccDNA formed (% repaired) was calculated by dividing the intensity of the ccc band by the sum of the intensities of the RrcDNA, linear RrcDNA and cccDNA bands. The absolute values are displayed above each lane number. Rrc, RrcDNA; rL, recombinant linear RrcDNA; ccc, cccDNA. All experiments were repeated twice with the same results. Source data are provided as a Source Data file.

    Techniques Used: Recombinant, Plasmid Preparation, Sequencing, Staining, Polyacrylamide Gel Electrophoresis, Nucleic Acid Electrophoresis, Purification, Countercurrent Chromatography

    Related Articles

    Electrophoresis:

    Article Title: Identification of Dermatophytes by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Analysis of Metalloproteinase-1
    Article Snippet: Subsequently, we purified the amplified PCR product and performed RFLP analysis. .. After restriction enzyme digestion, BsrD I (NEB, England), the samples were subjected to electrophoresis. ..

    Polymerase Chain Reaction:

    Article Title: A novel mutation in DAX1 causes delayed-onset adrenal insufficiency and incomplete hypogonadotropic hypogonadism
    Article Snippet: After identification of the mutation by DNA sequencing, BsrD I restriction digestion was used to detect the mutation in additional family members. .. Restriction enzyme digestions using BsrD I were performed at 60°C in a 30-μL reaction mixture containing 15 μL of PCR product 2F/2R, 20 U BsrD I (New England Biolabs, Beverly, Massachusetts, USA), 3 μL NEbuffer, and 0.3 μL BSA (all supplied by the manufacturer). .. The DNA digestion products were run on a 6% acrylamide gel.

    Incubation:

    Article Title: High-throughput, cost-effective verification of structural DNA assembly
    Article Snippet: For Afl II and Ava II digest of cloned DNA building blocks, the RCA reaction was diluted 4-fold with water. .. Digest formulations and incubation temperatures for Bsr DI, Afl II and Ava II (NEB) are given in Supplementary Table S2 . ..

    Antiviral Assay:

    Article Title: High-throughput, cost-effective verification of structural DNA assembly
    Article Snippet: For Afl II and Ava II digest of cloned DNA building blocks, the RCA reaction was diluted 4-fold with water. .. Digest formulations and incubation temperatures for Bsr DI, Afl II and Ava II (NEB) are given in Supplementary Table S2 . ..

    Purification:

    Article Title: Genome-wide Nucleotide-Resolution Mapping of DNA Replication Patterns, Single-Strand Breaks, and Lesions by GLOE-Seq
    Article Snippet: .. Validation of the GLOE-Seq Method Purified genomic DNA from yeast strain W303 was treated with 1 U/μg DNA of the relevant restriction or nicking enzymes, Bsr DI, Nb.Bsr DI or Not I (New England Biolabs) for 90 min at 65°C (Bsr DI and Nb.Bsr DI) or 37°C (NotI), dephosphorylated with 2 U/μg Antarctic Phosphatase (New England Biolabs) for 30 min at 37°C and purified using AMPure beads (Beckman Coulter). .. The purified DNA was quantified (Qubit, Life Technologies), and 2.5 μg were used for GLOE-Seq library preparation (steps 29-50) and sequencing.

    Article Title: Hepatitis B virus cccDNA is formed through distinct repair processes of each strand
    Article Snippet: The ligation efficiency was determined as described in Fig. . .. In brief, purified RrcDNA precursor and RrcDNA were digested with BsrDI (NEB). .. The digest was purified by phenol–chloroform extraction and resolved on a 10% (w/vol) urea-polyacrylamide gel electrophoresis (PAGE), which was subsequently stained with SYBRTM Gold (Thermo Fisher Scientific) to visualize the M1/P1 single-stranded oligos originated from RrcDNA precursor and M2/P2 single-stranded oligos produced by RrcDNA after BsrDI digestion as shown in Figs. b, , lanes 1–2.

    Nucleic Acid Electrophoresis:

    Article Title: Sequential, Divergent, and Cooperative Requirements of Foxl2a and Foxl2b in Ovary Development and Maintenance of Zebrafish
    Article Snippet: The gDNA fragments including the target site of the TALENs were amplified using foxl2a and foxl2b gene-specific primers ( ). .. After digestion with Bst N I (restriction enzyme cutting site in the spacer of foxl2a ) or Bsr D I (restriction enzyme cutting site in the spacer of foxl2b ) (New England Biolabs, Beverly, MA) respectively at 60° and 65° for 2 hr, the uncleaved DNA fragments were separated by gel electrophoresis and cloned into pMD-18T vector (Promega). .. Sequence alignments were generated to analyze whether or not the gDNA fragments from embryos injected with TALEN mRNAs were mutated.

    Clone Assay:

    Article Title: Sequential, Divergent, and Cooperative Requirements of Foxl2a and Foxl2b in Ovary Development and Maintenance of Zebrafish
    Article Snippet: The gDNA fragments including the target site of the TALENs were amplified using foxl2a and foxl2b gene-specific primers ( ). .. After digestion with Bst N I (restriction enzyme cutting site in the spacer of foxl2a ) or Bsr D I (restriction enzyme cutting site in the spacer of foxl2b ) (New England Biolabs, Beverly, MA) respectively at 60° and 65° for 2 hr, the uncleaved DNA fragments were separated by gel electrophoresis and cloned into pMD-18T vector (Promega). .. Sequence alignments were generated to analyze whether or not the gDNA fragments from embryos injected with TALEN mRNAs were mutated.

    Plasmid Preparation:

    Article Title: Sequential, Divergent, and Cooperative Requirements of Foxl2a and Foxl2b in Ovary Development and Maintenance of Zebrafish
    Article Snippet: The gDNA fragments including the target site of the TALENs were amplified using foxl2a and foxl2b gene-specific primers ( ). .. After digestion with Bst N I (restriction enzyme cutting site in the spacer of foxl2a ) or Bsr D I (restriction enzyme cutting site in the spacer of foxl2b ) (New England Biolabs, Beverly, MA) respectively at 60° and 65° for 2 hr, the uncleaved DNA fragments were separated by gel electrophoresis and cloned into pMD-18T vector (Promega). .. Sequence alignments were generated to analyze whether or not the gDNA fragments from embryos injected with TALEN mRNAs were mutated.

    Variant Assay:

    Article Title: Human-Specific SNP in Obesity Genes, Adrenergic Receptor Beta2 (ADRB2), Beta3 (ADRB3), and PPAR ?2 (PPARG), during Primate Evolution
    Article Snippet: However, the reverse primer region includes one more T in the sequence of H. sapiens , P. troglodytes , and M. mulatta from the Ensembl database, so the reverse primer including one more complementary nucleotide A was used ( ). .. ADRB2 fragment with the Arg16Gly variant site with 5 U of BsrD I (New England Bio Labs Co. Ltd.) at 65°C for 1.5 hrs. .. ADRB2 amplicon for the Gln27Glu substitution site (5 µl) was digested with 5 U of Ita I (Roche Applied Science Co. Ltd.) at 37°C for 1 hr.

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    New England Biolabs bsrdi
    Repair of HBV lesions on plus- and minus-strands require different sets of protein factors. a Characteristics of HBV rcDNA structure. b Schematic representation of the generation of recombinant HBV rcDNA <t>(RrcDNA)</t> substrates that contain all lesions (RrcDNA) or only lesions on the plus- (psl-RrcDNA) or minus-strands (msl-RrcDNA). HBV plasmids contains a plasmid backbone (gray) and either the minus strand sequence (black) or the plus-strand sequence (blue). <t>BsrDI</t> cleavage was used to monitor the generation of various RrcDNA substrates (BsrDI restriction sites are indicated by magenta arrows); green line, biotinylated flap; B, biotin; red line, RNA primer. Note that M1 and M2 are two oligos released from the minus-strands of RrcDNA precursor and RrcDNA after BsrDI digestion, whereas P1 and P2 are two oligos released from the plus-strands after BsrDI digestion. c Annealing products of psl-RrcDNA and msl-RrcDNA precursors were monitored by Sybr Safe staining. d Generation of various RrcDNA precursors was analyzed by formation of M2 and P2 oligos from the corresponding M1 and P1 oligos after BsrDI digestion and urea-PAGE gel electrophoresis followed by Sybr Gold staining. Please note that psl-RcDNA and msl-RrcDNA only contain annealed oligos on the plus-strand and minus strand, respectively. Almost complete conversion from P1 to P2 (psl-RcDNA, lanes 5–6), and M1 to M2 (msl-RcDNA, lanes 3–4) was observed. e All five human protein factors are required for repair of the lesions on the plus-strand. Psl-RrcDNA was mixed with combinations of purified proteins, and cccDNA formation was detected on agarose gels containing ethidium bromide (EtBr). Omission of factors is indicated by “−”. f FEN-1 and LIG1 are necessary and sufficient for repair of lesions on the minus strand. Omission of factors is indicated by “−”. The percentage of cccDNA formed (% repaired) was calculated by dividing the intensity of the ccc band by the sum of the intensities of the RrcDNA, linear RrcDNA and cccDNA bands. The absolute values are displayed above each lane number. Rrc, RrcDNA; rL, recombinant linear RrcDNA; ccc, cccDNA. All experiments were repeated twice with the same results. Source data are provided as a Source Data file.
    Bsrdi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bsrdi/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bsrdi - by Bioz Stars, 2021-04
    95/100 stars
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    Repair of HBV lesions on plus- and minus-strands require different sets of protein factors. a Characteristics of HBV rcDNA structure. b Schematic representation of the generation of recombinant HBV rcDNA (RrcDNA) substrates that contain all lesions (RrcDNA) or only lesions on the plus- (psl-RrcDNA) or minus-strands (msl-RrcDNA). HBV plasmids contains a plasmid backbone (gray) and either the minus strand sequence (black) or the plus-strand sequence (blue). BsrDI cleavage was used to monitor the generation of various RrcDNA substrates (BsrDI restriction sites are indicated by magenta arrows); green line, biotinylated flap; B, biotin; red line, RNA primer. Note that M1 and M2 are two oligos released from the minus-strands of RrcDNA precursor and RrcDNA after BsrDI digestion, whereas P1 and P2 are two oligos released from the plus-strands after BsrDI digestion. c Annealing products of psl-RrcDNA and msl-RrcDNA precursors were monitored by Sybr Safe staining. d Generation of various RrcDNA precursors was analyzed by formation of M2 and P2 oligos from the corresponding M1 and P1 oligos after BsrDI digestion and urea-PAGE gel electrophoresis followed by Sybr Gold staining. Please note that psl-RcDNA and msl-RrcDNA only contain annealed oligos on the plus-strand and minus strand, respectively. Almost complete conversion from P1 to P2 (psl-RcDNA, lanes 5–6), and M1 to M2 (msl-RcDNA, lanes 3–4) was observed. e All five human protein factors are required for repair of the lesions on the plus-strand. Psl-RrcDNA was mixed with combinations of purified proteins, and cccDNA formation was detected on agarose gels containing ethidium bromide (EtBr). Omission of factors is indicated by “−”. f FEN-1 and LIG1 are necessary and sufficient for repair of lesions on the minus strand. Omission of factors is indicated by “−”. The percentage of cccDNA formed (% repaired) was calculated by dividing the intensity of the ccc band by the sum of the intensities of the RrcDNA, linear RrcDNA and cccDNA bands. The absolute values are displayed above each lane number. Rrc, RrcDNA; rL, recombinant linear RrcDNA; ccc, cccDNA. All experiments were repeated twice with the same results. Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: Hepatitis B virus cccDNA is formed through distinct repair processes of each strand

    doi: 10.1038/s41467-021-21850-9

    Figure Lengend Snippet: Repair of HBV lesions on plus- and minus-strands require different sets of protein factors. a Characteristics of HBV rcDNA structure. b Schematic representation of the generation of recombinant HBV rcDNA (RrcDNA) substrates that contain all lesions (RrcDNA) or only lesions on the plus- (psl-RrcDNA) or minus-strands (msl-RrcDNA). HBV plasmids contains a plasmid backbone (gray) and either the minus strand sequence (black) or the plus-strand sequence (blue). BsrDI cleavage was used to monitor the generation of various RrcDNA substrates (BsrDI restriction sites are indicated by magenta arrows); green line, biotinylated flap; B, biotin; red line, RNA primer. Note that M1 and M2 are two oligos released from the minus-strands of RrcDNA precursor and RrcDNA after BsrDI digestion, whereas P1 and P2 are two oligos released from the plus-strands after BsrDI digestion. c Annealing products of psl-RrcDNA and msl-RrcDNA precursors were monitored by Sybr Safe staining. d Generation of various RrcDNA precursors was analyzed by formation of M2 and P2 oligos from the corresponding M1 and P1 oligos after BsrDI digestion and urea-PAGE gel electrophoresis followed by Sybr Gold staining. Please note that psl-RcDNA and msl-RrcDNA only contain annealed oligos on the plus-strand and minus strand, respectively. Almost complete conversion from P1 to P2 (psl-RcDNA, lanes 5–6), and M1 to M2 (msl-RcDNA, lanes 3–4) was observed. e All five human protein factors are required for repair of the lesions on the plus-strand. Psl-RrcDNA was mixed with combinations of purified proteins, and cccDNA formation was detected on agarose gels containing ethidium bromide (EtBr). Omission of factors is indicated by “−”. f FEN-1 and LIG1 are necessary and sufficient for repair of lesions on the minus strand. Omission of factors is indicated by “−”. The percentage of cccDNA formed (% repaired) was calculated by dividing the intensity of the ccc band by the sum of the intensities of the RrcDNA, linear RrcDNA and cccDNA bands. The absolute values are displayed above each lane number. Rrc, RrcDNA; rL, recombinant linear RrcDNA; ccc, cccDNA. All experiments were repeated twice with the same results. Source data are provided as a Source Data file.

    Article Snippet: In brief, purified RrcDNA precursor and RrcDNA were digested with BsrDI (NEB).

    Techniques: Recombinant, Plasmid Preparation, Sequencing, Staining, Polyacrylamide Gel Electrophoresis, Nucleic Acid Electrophoresis, Purification, Countercurrent Chromatography

    Identification of the DAX1 missense mutation. ( a ) Chromatogram showing the I439S missense mutation in exon 2 of DAX1 . A guanine is present at nucleotide position 1316 in the patient (arrow), whereas a thymine is present at the equivalent position in a genomic sequence from a control subject. ( b ) The thymine-to-guanine nucleotide change creates a novel BsrD I site. PCR products from the patient, his mother, his normal brother, his father, and a normal control were digested with this restriction enzyme (lanes 1–5, respectively). The mutated fragment, characterized by a band 13 bp shorter than the wild-type fragment, is present in the patient and his mother (arrows).

    Journal: Journal of Clinical Investigation

    Article Title: A novel mutation in DAX1 causes delayed-onset adrenal insufficiency and incomplete hypogonadotropic hypogonadism

    doi:

    Figure Lengend Snippet: Identification of the DAX1 missense mutation. ( a ) Chromatogram showing the I439S missense mutation in exon 2 of DAX1 . A guanine is present at nucleotide position 1316 in the patient (arrow), whereas a thymine is present at the equivalent position in a genomic sequence from a control subject. ( b ) The thymine-to-guanine nucleotide change creates a novel BsrD I site. PCR products from the patient, his mother, his normal brother, his father, and a normal control were digested with this restriction enzyme (lanes 1–5, respectively). The mutated fragment, characterized by a band 13 bp shorter than the wild-type fragment, is present in the patient and his mother (arrows).

    Article Snippet: Restriction enzyme digestions using BsrD I were performed at 60°C in a 30-μL reaction mixture containing 15 μL of PCR product 2F/2R, 20 U BsrD I (New England Biolabs, Beverly, Massachusetts, USA), 3 μL NEbuffer, and 0.3 μL BSA (all supplied by the manufacturer).

    Techniques: Mutagenesis, Sequencing, Polymerase Chain Reaction

    Nucleotide sequences in ADRB2 of humans and NHPs. Primers 16HF and 16HRc (Rc means the complementary sequence of reverse primer) indicate a primer set used for the PCR to amplify the region for the 16 th amino acid in hominoids. One nucleotide of primer 16HF was changed to create the restriction site of BsrD I. Primers 27MF and 27MRc indicate a primer set used for the PCR to amplify the region for the 27 th amino acid in macaques. The underlines show the restriction site ( GCAATGNN ) with BsrD I for the 16 th amino acid and the restriction site (GCNGC) with Ita I for the 27 th amino acid. The nucleotide sequences for hominoids were determined from G. gorilla (DDBJ Accession No. AB669098), P. pygmaeus (AB669099) and H. agilis (AB669100), and obtained from the Ensembl database for ADRB2 of H. sapiens (ENSG00000169252) and P. troglodytes (ENSPTRG00000017391). All macaques, M. fascicularis , M. fuscata , M. nemestrina , M. radiata (AB669101∼AB669104, respectively) and M. mulatta (ENSMMUG 00000002214), show the same sequence.

    Journal: PLoS ONE

    Article Title: Human-Specific SNP in Obesity Genes, Adrenergic Receptor Beta2 (ADRB2), Beta3 (ADRB3), and PPAR ?2 (PPARG), during Primate Evolution

    doi: 10.1371/journal.pone.0043461

    Figure Lengend Snippet: Nucleotide sequences in ADRB2 of humans and NHPs. Primers 16HF and 16HRc (Rc means the complementary sequence of reverse primer) indicate a primer set used for the PCR to amplify the region for the 16 th amino acid in hominoids. One nucleotide of primer 16HF was changed to create the restriction site of BsrD I. Primers 27MF and 27MRc indicate a primer set used for the PCR to amplify the region for the 27 th amino acid in macaques. The underlines show the restriction site ( GCAATGNN ) with BsrD I for the 16 th amino acid and the restriction site (GCNGC) with Ita I for the 27 th amino acid. The nucleotide sequences for hominoids were determined from G. gorilla (DDBJ Accession No. AB669098), P. pygmaeus (AB669099) and H. agilis (AB669100), and obtained from the Ensembl database for ADRB2 of H. sapiens (ENSG00000169252) and P. troglodytes (ENSPTRG00000017391). All macaques, M. fascicularis , M. fuscata , M. nemestrina , M. radiata (AB669101∼AB669104, respectively) and M. mulatta (ENSMMUG 00000002214), show the same sequence.

    Article Snippet: ADRB2 fragment with the Arg16Gly variant site with 5 U of BsrD I (New England Bio Labs Co. Ltd.) at 65°C for 1.5 hrs.

    Techniques: Sequencing, Polymerase Chain Reaction

    All hominoids had Gly16 allele in ADRB2 . A) Restriction map of ADRB2 for the 16 th amino acid digested with BsrD I ( GCAATGNN ). This restriction map was predicted from the nucleotide sequences of hominoids ( Fig. 1 ). B) RFLP patterns of PCR products of ADRB2 for the 16 th amino acid digested with BsrD I in hominoids. Lane 1: PCR product of a human; not digested (200 bp). Lane 2: Fragments of human Arg16/Gly16 (130,108 and 56 bp (22 and 14 bp fragments were undetectable)). Lane 3 to lane 6: Fragments from P. troglodytes , G. gorilla , P. pygmaeus , and H. agilis , respectively (108 and 22 bp instead of 130 bp).

    Journal: PLoS ONE

    Article Title: Human-Specific SNP in Obesity Genes, Adrenergic Receptor Beta2 (ADRB2), Beta3 (ADRB3), and PPAR ?2 (PPARG), during Primate Evolution

    doi: 10.1371/journal.pone.0043461

    Figure Lengend Snippet: All hominoids had Gly16 allele in ADRB2 . A) Restriction map of ADRB2 for the 16 th amino acid digested with BsrD I ( GCAATGNN ). This restriction map was predicted from the nucleotide sequences of hominoids ( Fig. 1 ). B) RFLP patterns of PCR products of ADRB2 for the 16 th amino acid digested with BsrD I in hominoids. Lane 1: PCR product of a human; not digested (200 bp). Lane 2: Fragments of human Arg16/Gly16 (130,108 and 56 bp (22 and 14 bp fragments were undetectable)). Lane 3 to lane 6: Fragments from P. troglodytes , G. gorilla , P. pygmaeus , and H. agilis , respectively (108 and 22 bp instead of 130 bp).

    Article Snippet: ADRB2 fragment with the Arg16Gly variant site with 5 U of BsrD I (New England Bio Labs Co. Ltd.) at 65°C for 1.5 hrs.

    Techniques: Polymerase Chain Reaction

    Restriction fragment length polymorphism patterns obtained upon DNA digestion with BsrD I. M: 100-base pair (bp) marker, 1: Microsporumcanis (IFM45829), 2: M. gypseum (IFM-5292), 3: Trichophyton mentagrophytes var. interdigitale (IFM-48155), 4: T. mentagrophytes var. mentagrophytes (CBS113880), 5: T. rubrum (ATCC28188), 6: T. tonsurans (CBS109036).

    Journal: Annals of Dermatology

    Article Title: Identification of Dermatophytes by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Analysis of Metalloproteinase-1

    doi: 10.5021/ad.2014.26.3.338

    Figure Lengend Snippet: Restriction fragment length polymorphism patterns obtained upon DNA digestion with BsrD I. M: 100-base pair (bp) marker, 1: Microsporumcanis (IFM45829), 2: M. gypseum (IFM-5292), 3: Trichophyton mentagrophytes var. interdigitale (IFM-48155), 4: T. mentagrophytes var. mentagrophytes (CBS113880), 5: T. rubrum (ATCC28188), 6: T. tonsurans (CBS109036).

    Article Snippet: After restriction enzyme digestion, BsrD I (NEB, England), the samples were subjected to electrophoresis.

    Techniques: Marker