bsmfi  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95
    Name:
    BsmFI
    Description:
    BsmFI 500 units
    Catalog Number:
    r0572l
    Price:
    290
    Size:
    500 units
    Category:
    Restriction Enzymes
    Buy from Supplier


    Structured Review

    New England Biolabs bsmfi
    BsmFI
    BsmFI 500 units
    https://www.bioz.com/result/bsmfi/product/New England Biolabs
    Average 95 stars, based on 8 article reviews
    Price from $9.99 to $1999.99
    bsmfi - by Bioz Stars, 2020-09
    95/100 stars

    Images

    1) Product Images from "Alanine-Threonine Polymorphism of Helicobacter pylori RpoB Is Correlated with Differential Induction of Interleukin-8 in MKN45 Cells"

    Article Title: Alanine-Threonine Polymorphism of Helicobacter pylori RpoB Is Correlated with Differential Induction of Interleukin-8 in MKN45 Cells

    Journal: Journal of Clinical Microbiology

    doi: 10.1128/JCM.42.8.3518-3524.2004

    Differentiation of RpoB Thr and RpoB Ala type H. pylori strains by PCR-restriction fragment length polymorphism analysis (with BsmFI) of rpoB DNA. Amplified rpoB DNAs (458 bp) of H. pylori were digested with BsmFI and electrophoresed in a 3% agarose gel. DNAs from the RpoB Thr types were digested (lanes 4 and 5 [248 and 210 bp] and lanes 6 and 7 [248, 116, and 94 bp]), while DNAs from the RpoB Ala types were not (lanes 1 to 3 [458 bp]). Lane M, size marker (φX174 replicative-form DNA digested with HaeIII). The numbers next to the gels are in base pairs.
    Figure Legend Snippet: Differentiation of RpoB Thr and RpoB Ala type H. pylori strains by PCR-restriction fragment length polymorphism analysis (with BsmFI) of rpoB DNA. Amplified rpoB DNAs (458 bp) of H. pylori were digested with BsmFI and electrophoresed in a 3% agarose gel. DNAs from the RpoB Thr types were digested (lanes 4 and 5 [248 and 210 bp] and lanes 6 and 7 [248, 116, and 94 bp]), while DNAs from the RpoB Ala types were not (lanes 1 to 3 [458 bp]). Lane M, size marker (φX174 replicative-form DNA digested with HaeIII). The numbers next to the gels are in base pairs.

    Techniques Used: Polymerase Chain Reaction, Amplification, Agarose Gel Electrophoresis, Marker

    2) Product Images from "Semicircular canal morphogenesis in the zebrafish inner ear requires the function of gpr126 (lauscher), an adhesion class G protein-coupled receptor gene"

    Article Title: Semicircular canal morphogenesis in the zebrafish inner ear requires the function of gpr126 (lauscher), an adhesion class G protein-coupled receptor gene

    Journal: Development (Cambridge, England)

    doi: 10.1242/dev.098061

    Positional cloning, identification and confirmation of mutations in gpr126. ( A ) Genetic map of the lau locus. Numbers of meiotic recombinants for the flanking SSLP markers and SNP markers in slc25a27, schnurri2 and galnt14 are shown. ( B ) Sequence analysis of gpr126 cDNA in wild type (upper panels) and lau mutants (lower panels), with predicted changes to the coding sequence. ( C ) Schematic diagram of the Gpr126 protein, with its conserved domains: CUB (Complement C1r/C1s, Uegf, BMP1), PTX (Pentraxin), HBD (hormone binding domain), GAIN (GPCR autoproteolysis inducing) domain, GPS (GPCR proteolytic site) motif and 7TM (7-transmembrane) domain (not to scale). Positions of the mutations are shown. ( D ) Amino acid comparison of the fourth transmembrane (TMIV) region from eight zebrafish adhesion class GPCRs, showing the conserved hydrophobic residue at position 963 (mutated in tb233c ), and the highly conserved proline (P) at position 969 (mutated in tk256a ) (asterisks). ( E ) Genotyping of lau mutant fish by restriction digest of PCR-amplified genomic DNA. In tb233c , the mutation eliminated an Sfa N1 site; in tk256a , the mutation eliminated a Bsm F1 site; in the fr24 allele, a Bfa 1 site was gained. (F,G) gpr126 morpholino injection recapitulates the lau mutant ear phenotype. ( F ) Wild-type ( nac ) embryos (left hand panels) injected with 5 ng control morpholino exhibit normal ear morphology at 5 dpf (a,c), and low vcanb expression at 5 dpf (e). Wild-type ( nac ) embryos injected with 5 ng gpr126 morpholino (right hand panels) have abnormal projection outgrowth (b,d). The lateral projection (lp) is enlarged, and the posterior projection (pp) in this ear has grown past the lateral projection without fusing. Expression of vcanb is upregulated (f). ( G ) RT-PCR analysis of gpr126 mRNA processing of the 19th exon in gpr126 morpholino-injected, or in control (mismatched) morpholino-injected, embryos at 5 dpf. Sequencing confirmed two aberrant splice variants. Panels c,d are dorsal views, anterior towards the top. Scale bars: 200 μm in a,b; 50 μm in c,d; 50 μm in e,f.
    Figure Legend Snippet: Positional cloning, identification and confirmation of mutations in gpr126. ( A ) Genetic map of the lau locus. Numbers of meiotic recombinants for the flanking SSLP markers and SNP markers in slc25a27, schnurri2 and galnt14 are shown. ( B ) Sequence analysis of gpr126 cDNA in wild type (upper panels) and lau mutants (lower panels), with predicted changes to the coding sequence. ( C ) Schematic diagram of the Gpr126 protein, with its conserved domains: CUB (Complement C1r/C1s, Uegf, BMP1), PTX (Pentraxin), HBD (hormone binding domain), GAIN (GPCR autoproteolysis inducing) domain, GPS (GPCR proteolytic site) motif and 7TM (7-transmembrane) domain (not to scale). Positions of the mutations are shown. ( D ) Amino acid comparison of the fourth transmembrane (TMIV) region from eight zebrafish adhesion class GPCRs, showing the conserved hydrophobic residue at position 963 (mutated in tb233c ), and the highly conserved proline (P) at position 969 (mutated in tk256a ) (asterisks). ( E ) Genotyping of lau mutant fish by restriction digest of PCR-amplified genomic DNA. In tb233c , the mutation eliminated an Sfa N1 site; in tk256a , the mutation eliminated a Bsm F1 site; in the fr24 allele, a Bfa 1 site was gained. (F,G) gpr126 morpholino injection recapitulates the lau mutant ear phenotype. ( F ) Wild-type ( nac ) embryos (left hand panels) injected with 5 ng control morpholino exhibit normal ear morphology at 5 dpf (a,c), and low vcanb expression at 5 dpf (e). Wild-type ( nac ) embryos injected with 5 ng gpr126 morpholino (right hand panels) have abnormal projection outgrowth (b,d). The lateral projection (lp) is enlarged, and the posterior projection (pp) in this ear has grown past the lateral projection without fusing. Expression of vcanb is upregulated (f). ( G ) RT-PCR analysis of gpr126 mRNA processing of the 19th exon in gpr126 morpholino-injected, or in control (mismatched) morpholino-injected, embryos at 5 dpf. Sequencing confirmed two aberrant splice variants. Panels c,d are dorsal views, anterior towards the top. Scale bars: 200 μm in a,b; 50 μm in c,d; 50 μm in e,f.

    Techniques Used: Clone Assay, Sequencing, Binding Assay, Mutagenesis, Fluorescence In Situ Hybridization, Polymerase Chain Reaction, Amplification, Injection, Expressing, Reverse Transcription Polymerase Chain Reaction

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Alanine-Threonine Polymorphism of Helicobacter pylori RpoB Is Correlated with Differential Induction of Interleukin-8 in MKN45 Cells
    Article Snippet: .. Ten microliters of the rpoB PCR products was transferred to a fresh microcentrifuge tube and digested with BsmFI (R0572S; New England Biolabs, Beverly, Mass.), according to the supplier's instruction. ..

    Article Title: Genetic Control of Resistance to Trypanosoma brucei brucei Infection in Mice
    Article Snippet: .. Polymorphism of SNPs was tested by restriction analysis after PCR reaction using following restriction enzymes (New England BioLabs, Ipswich, MA): HpyAV for rs48212577 (14,13 µl of PCR product, 2 U (1 µl) of HpyAV, 1.7 µl of 10x NEB buffer 4 [200 mM Tris-acetate, 500 mM Potassium Acetate, 100 mM Magnesium Acetate, 10 mM Dithiothreitol, pH 7.9], 0.17 µl of 10 mg/ml BSA (bovine serum albumin), 37°C, o/n); HinfI for rs4229232 (14.8 µl of PCR product, 5 U (0,5 µl) of HinfI, 1.7 µl of 10x NEB buffer 4, 37°C, o/n); BsmFI for rs50154157 (14,13 µl of PCR product, 2 U (1 µl) of BsmFI, 1.7 µl of 10x NEB buffer 4, 0.17 µl of 10 mg/ml BSA, 65°C, o/n), and Tsp509I for rs50776991 (14,8 µl of PCR product, 2 U (0,5 µl) of Tsp509I, 1.7 µl of 10x NEB buffer 1 [100 mM Bis-Tris-propane-HCl, 100 mM MgCl2, 10 mM Dithiothreitol, pH 7.0], 65°C, o/n). .. The products were electrophoresed in 3% agarose gel containing 80% of MetaPhor® Agarose (Cambrex Bio Science Rockland, Inc., Rockland, ME) and 20% of UltraPure™ Agarose (Invitrogen, Carlsbad, CA) for 15 min to 2 h at 150 V.

    Amplification:

    Article Title: Modified Serial Analysis of Gene Expression Method for Construction of Gene Expression Profiles of Microbial Eukaryotic Species
    Article Snippet: .. The precipitated amplification products were digested with the tagging enzyme BsmFI in a 50-μl reaction mixture containing 0.2 mM each dNTP, 1× NEB Buffer 4, 1× bovine serum albumin (BSA), and 2 U of BsmFI (NEB) for 1 h at 65°C. .. Restriction products were filled in by the addition of 3 U of T4 polymerase (NEB) to the reaction mixture and incubated for 20 min at 12°C.

    Agarose Gel Electrophoresis:

    Article Title: Semicircular canal morphogenesis in the zebrafish inner ear requires the function of gpr126 (lauscher), an adhesion class G protein-coupled receptor gene
    Article Snippet: .. The resulting tb233c, tk256a and fr24 products were digested with the restriction enzymes Sfa NI, Bsm FI and Bfa I (New England Biolabs), respectively, for 5 hours at 37°C and separated on a 2% TBE agarose gel. .. Morpholino injection A morpholino oligonucleotide (Gene Tools) was designed to target the splice donor site of exon 19 of gpr126 ; a 5-base mismatch morpholino was used as a control (see supplementary material Table S1 for sequences).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95
    New England Biolabs bsmfi
    Differentiation of RpoB Thr and RpoB Ala type H. pylori strains by <t>PCR-restriction</t> fragment length polymorphism analysis (with <t>BsmFI)</t> of rpoB DNA. Amplified rpoB DNAs (458 bp) of H. pylori were digested with BsmFI and electrophoresed in a 3% agarose gel. DNAs from the RpoB Thr types were digested (lanes 4 and 5 [248 and 210 bp] and lanes 6 and 7 [248, 116, and 94 bp]), while DNAs from the RpoB Ala types were not (lanes 1 to 3 [458 bp]). Lane M, size marker (φX174 replicative-form DNA digested with HaeIII). The numbers next to the gels are in base pairs.
    Bsmfi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bsmfi/product/New England Biolabs
    Average 95 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    bsmfi - by Bioz Stars, 2020-09
    95/100 stars
      Buy from Supplier

    Image Search Results


    Differentiation of RpoB Thr and RpoB Ala type H. pylori strains by PCR-restriction fragment length polymorphism analysis (with BsmFI) of rpoB DNA. Amplified rpoB DNAs (458 bp) of H. pylori were digested with BsmFI and electrophoresed in a 3% agarose gel. DNAs from the RpoB Thr types were digested (lanes 4 and 5 [248 and 210 bp] and lanes 6 and 7 [248, 116, and 94 bp]), while DNAs from the RpoB Ala types were not (lanes 1 to 3 [458 bp]). Lane M, size marker (φX174 replicative-form DNA digested with HaeIII). The numbers next to the gels are in base pairs.

    Journal: Journal of Clinical Microbiology

    Article Title: Alanine-Threonine Polymorphism of Helicobacter pylori RpoB Is Correlated with Differential Induction of Interleukin-8 in MKN45 Cells

    doi: 10.1128/JCM.42.8.3518-3524.2004

    Figure Lengend Snippet: Differentiation of RpoB Thr and RpoB Ala type H. pylori strains by PCR-restriction fragment length polymorphism analysis (with BsmFI) of rpoB DNA. Amplified rpoB DNAs (458 bp) of H. pylori were digested with BsmFI and electrophoresed in a 3% agarose gel. DNAs from the RpoB Thr types were digested (lanes 4 and 5 [248 and 210 bp] and lanes 6 and 7 [248, 116, and 94 bp]), while DNAs from the RpoB Ala types were not (lanes 1 to 3 [458 bp]). Lane M, size marker (φX174 replicative-form DNA digested with HaeIII). The numbers next to the gels are in base pairs.

    Article Snippet: Ten microliters of the rpoB PCR products was transferred to a fresh microcentrifuge tube and digested with BsmFI (R0572S; New England Biolabs, Beverly, Mass.), according to the supplier's instruction.

    Techniques: Polymerase Chain Reaction, Amplification, Agarose Gel Electrophoresis, Marker

    Positional cloning, identification and confirmation of mutations in gpr126. ( A ) Genetic map of the lau locus. Numbers of meiotic recombinants for the flanking SSLP markers and SNP markers in slc25a27, schnurri2 and galnt14 are shown. ( B ) Sequence analysis of gpr126 cDNA in wild type (upper panels) and lau mutants (lower panels), with predicted changes to the coding sequence. ( C ) Schematic diagram of the Gpr126 protein, with its conserved domains: CUB (Complement C1r/C1s, Uegf, BMP1), PTX (Pentraxin), HBD (hormone binding domain), GAIN (GPCR autoproteolysis inducing) domain, GPS (GPCR proteolytic site) motif and 7TM (7-transmembrane) domain (not to scale). Positions of the mutations are shown. ( D ) Amino acid comparison of the fourth transmembrane (TMIV) region from eight zebrafish adhesion class GPCRs, showing the conserved hydrophobic residue at position 963 (mutated in tb233c ), and the highly conserved proline (P) at position 969 (mutated in tk256a ) (asterisks). ( E ) Genotyping of lau mutant fish by restriction digest of PCR-amplified genomic DNA. In tb233c , the mutation eliminated an Sfa N1 site; in tk256a , the mutation eliminated a Bsm F1 site; in the fr24 allele, a Bfa 1 site was gained. (F,G) gpr126 morpholino injection recapitulates the lau mutant ear phenotype. ( F ) Wild-type ( nac ) embryos (left hand panels) injected with 5 ng control morpholino exhibit normal ear morphology at 5 dpf (a,c), and low vcanb expression at 5 dpf (e). Wild-type ( nac ) embryos injected with 5 ng gpr126 morpholino (right hand panels) have abnormal projection outgrowth (b,d). The lateral projection (lp) is enlarged, and the posterior projection (pp) in this ear has grown past the lateral projection without fusing. Expression of vcanb is upregulated (f). ( G ) RT-PCR analysis of gpr126 mRNA processing of the 19th exon in gpr126 morpholino-injected, or in control (mismatched) morpholino-injected, embryos at 5 dpf. Sequencing confirmed two aberrant splice variants. Panels c,d are dorsal views, anterior towards the top. Scale bars: 200 μm in a,b; 50 μm in c,d; 50 μm in e,f.

    Journal: Development (Cambridge, England)

    Article Title: Semicircular canal morphogenesis in the zebrafish inner ear requires the function of gpr126 (lauscher), an adhesion class G protein-coupled receptor gene

    doi: 10.1242/dev.098061

    Figure Lengend Snippet: Positional cloning, identification and confirmation of mutations in gpr126. ( A ) Genetic map of the lau locus. Numbers of meiotic recombinants for the flanking SSLP markers and SNP markers in slc25a27, schnurri2 and galnt14 are shown. ( B ) Sequence analysis of gpr126 cDNA in wild type (upper panels) and lau mutants (lower panels), with predicted changes to the coding sequence. ( C ) Schematic diagram of the Gpr126 protein, with its conserved domains: CUB (Complement C1r/C1s, Uegf, BMP1), PTX (Pentraxin), HBD (hormone binding domain), GAIN (GPCR autoproteolysis inducing) domain, GPS (GPCR proteolytic site) motif and 7TM (7-transmembrane) domain (not to scale). Positions of the mutations are shown. ( D ) Amino acid comparison of the fourth transmembrane (TMIV) region from eight zebrafish adhesion class GPCRs, showing the conserved hydrophobic residue at position 963 (mutated in tb233c ), and the highly conserved proline (P) at position 969 (mutated in tk256a ) (asterisks). ( E ) Genotyping of lau mutant fish by restriction digest of PCR-amplified genomic DNA. In tb233c , the mutation eliminated an Sfa N1 site; in tk256a , the mutation eliminated a Bsm F1 site; in the fr24 allele, a Bfa 1 site was gained. (F,G) gpr126 morpholino injection recapitulates the lau mutant ear phenotype. ( F ) Wild-type ( nac ) embryos (left hand panels) injected with 5 ng control morpholino exhibit normal ear morphology at 5 dpf (a,c), and low vcanb expression at 5 dpf (e). Wild-type ( nac ) embryos injected with 5 ng gpr126 morpholino (right hand panels) have abnormal projection outgrowth (b,d). The lateral projection (lp) is enlarged, and the posterior projection (pp) in this ear has grown past the lateral projection without fusing. Expression of vcanb is upregulated (f). ( G ) RT-PCR analysis of gpr126 mRNA processing of the 19th exon in gpr126 morpholino-injected, or in control (mismatched) morpholino-injected, embryos at 5 dpf. Sequencing confirmed two aberrant splice variants. Panels c,d are dorsal views, anterior towards the top. Scale bars: 200 μm in a,b; 50 μm in c,d; 50 μm in e,f.

    Article Snippet: The resulting tb233c, tk256a and fr24 products were digested with the restriction enzymes Sfa NI, Bsm FI and Bfa I (New England Biolabs), respectively, for 5 hours at 37°C and separated on a 2% TBE agarose gel.

    Techniques: Clone Assay, Sequencing, Binding Assay, Mutagenesis, Fluorescence In Situ Hybridization, Polymerase Chain Reaction, Amplification, Injection, Expressing, Reverse Transcription Polymerase Chain Reaction