msli  (New England Biolabs)


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  • 94
    Name:
    MslI
    Description:

    Catalog Number:
    R0571
    Price:
    296
    Category:
    Restriction Enzymes
    Applications:
    DNA Manipulation
    Size:
    2500 units
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    Structured Review

    New England Biolabs msli
    MslI

    https://www.bioz.com/result/msli/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    msli - by Bioz Stars, 2021-08
    94/100 stars

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    Related Articles

    Next-Generation Sequencing:

    Article Title: Genetic diversity and population structure of trifoliate yam (Dioscorea dumetorum Kunth) in Cameroon revealed by genotyping-by-sequencing (GBS)
    Article Snippet: .. Preparation of libraries for next-generation sequencing A total of 200 ng of genomic DNA for each sample were digested with 1 Unit MslI (New England Biolabs, NEB) in 1x NEB4 buffer in 30 μl volume for 1 h at 37 °C. ..

    Amplification:

    Article Title: Erratum to: ‘The Memory Aid study: protocol for a randomized controlled clinical trial evaluating the effect of computer-based working memory training in elderly patients with mild cognitive impairment (MCI)’
    Article Snippet: .. Then 15 μl of the amplification PCR products will be digested directly by 2.5 U of restriction enzymes MslI (R0571S, New England Biolabs, Beverly, MA) for 2 hours at 37 °C, and by Hae II (R0107S, New England Biolabs, Beverly, MA, USA) and Afl III (R0541S, New England Biolabs, Beverly, MA, USA) overnight at 37 °C. ..

    Article Title: Within-patient evolution to piperacillin/tazobactam resistance in a clinical isolate of Escherichia coli due to IS26-mediated amplification of blaTEM-1B
    Article Snippet: .. Restriction Enzyme DigestionRestriction fragment length polymorphism (RFLP) analysis of 1 μg of 16S rRNA PCR amplicon from the 10 putative clonal isolates were digested with AlwNI, PpuHI and MslI (all New England Biolabs, USA) and 1 μg of long fragment genomic DNA extracts of 153964, 152025, 190693 and 169757 were digested with SpeI and MslI (both New England Biolabs, USA) for 1 hour at 37°C. ..

    Polymerase Chain Reaction:

    Article Title: Erratum to: ‘The Memory Aid study: protocol for a randomized controlled clinical trial evaluating the effect of computer-based working memory training in elderly patients with mild cognitive impairment (MCI)’
    Article Snippet: .. Then 15 μl of the amplification PCR products will be digested directly by 2.5 U of restriction enzymes MslI (R0571S, New England Biolabs, Beverly, MA) for 2 hours at 37 °C, and by Hae II (R0107S, New England Biolabs, Beverly, MA, USA) and Afl III (R0541S, New England Biolabs, Beverly, MA, USA) overnight at 37 °C. ..

    Article Title: ATP-dependent helicase activity is dispensable for the physiological functions of Recql4
    Article Snippet: .. Genotyping Genotyping of the K525A mutants was performed by PCR using the following primers: mRecql4 K525A MO36-F9: 5’-TAGACCTTATGAAACCTCAAAGCC-3’ and mRecql4 K525A MO36-R3: 5’- AGAACATTGGGCATTCGGC-3’ to yield a 591bp product, which was then digested with MslI (NEB) restriction enzyme to generate two fragments of 416 and 175bp for the WT or three fragments of 361, 175 and 55bp for the K525A mutant. ..

    Article Title: Within-patient evolution to piperacillin/tazobactam resistance in a clinical isolate of Escherichia coli due to IS26-mediated amplification of blaTEM-1B
    Article Snippet: .. Restriction Enzyme DigestionRestriction fragment length polymorphism (RFLP) analysis of 1 μg of 16S rRNA PCR amplicon from the 10 putative clonal isolates were digested with AlwNI, PpuHI and MslI (all New England Biolabs, USA) and 1 μg of long fragment genomic DNA extracts of 153964, 152025, 190693 and 169757 were digested with SpeI and MslI (both New England Biolabs, USA) for 1 hour at 37°C. ..

    Article Title: Expression of sterol regulatory element-binding transcription factor (SREBF) 2 and SREBF cleavage-activating protein (SCAP) in human atheroma and the association of their allelic variants with sudden cardiac death
    Article Snippet: .. The PCR-amplified DNA fragment was incubated for 16 hours with 10 U of MslI (New England Biolabs). ..

    Article Title: Rothmund-Thomson Syndrome-like RECQL4 truncating mutations cause a haploinsufficient low bone mass phenotype in mice
    Article Snippet: .. Genotyping Genotyping of the G522Efs mutants was determined by PCR using the following primers: mRecql4 K525A MO36-R3: 5’-AGAACATTGGGCATTCGGC-3’ and mRecql4 K525A MO36-F9: 5’-TAGACCTTATGAAACCTCAAAGCC-3’ to obtain a 591bp product, which was then digested with MslI (NEB) to generate three fragments of 347, 175 and 71bp for the G522Efs mutant; or two fragments of 416 and 175bp for the WT. ..

    Mutagenesis:

    Article Title: ATP-dependent helicase activity is dispensable for the physiological functions of Recql4
    Article Snippet: .. Genotyping Genotyping of the K525A mutants was performed by PCR using the following primers: mRecql4 K525A MO36-F9: 5’-TAGACCTTATGAAACCTCAAAGCC-3’ and mRecql4 K525A MO36-R3: 5’- AGAACATTGGGCATTCGGC-3’ to yield a 591bp product, which was then digested with MslI (NEB) restriction enzyme to generate two fragments of 416 and 175bp for the WT or three fragments of 361, 175 and 55bp for the K525A mutant. ..

    Article Title: Rothmund-Thomson Syndrome-like RECQL4 truncating mutations cause a haploinsufficient low bone mass phenotype in mice
    Article Snippet: .. Genotyping Genotyping of the G522Efs mutants was determined by PCR using the following primers: mRecql4 K525A MO36-R3: 5’-AGAACATTGGGCATTCGGC-3’ and mRecql4 K525A MO36-F9: 5’-TAGACCTTATGAAACCTCAAAGCC-3’ to obtain a 591bp product, which was then digested with MslI (NEB) to generate three fragments of 347, 175 and 71bp for the G522Efs mutant; or two fragments of 416 and 175bp for the WT. ..

    Incubation:

    Article Title: Expression of sterol regulatory element-binding transcription factor (SREBF) 2 and SREBF cleavage-activating protein (SCAP) in human atheroma and the association of their allelic variants with sudden cardiac death
    Article Snippet: .. The PCR-amplified DNA fragment was incubated for 16 hours with 10 U of MslI (New England Biolabs). ..

    Ligation:

    Article Title: Comparative Phylogeography of Veronica spicata and V. longifolia (Plantaginaceae) Across Europe: Integrating Hybridization and Polyploidy in Phylogeography
    Article Snippet: .. Briefly, 200 ng genomic DNA were included in restriction-digestion with one unit MslI (New England Biolabs, NEB) in 30 μl (1× NEB4 buffer) at 37°C for 1 h and heat inactivated at 80°C for 20 min. Adaptor ligation was conducted with 15 μl of restriction product and 3 μl ligation adapters (Ovation Rapid DR Multiplex System, Nugen Technologies, Leek, Netherlands) in 12 μl of master mix (4.6 μl D1 water, 6 μlL1 ligation buffer mix, and 1.5 μlL3 ligation enzyme) at 25°C for 15 min and heat inactivated at 65°C for 10 min. ..

    Multiplex Assay:

    Article Title: Comparative Phylogeography of Veronica spicata and V. longifolia (Plantaginaceae) Across Europe: Integrating Hybridization and Polyploidy in Phylogeography
    Article Snippet: .. Briefly, 200 ng genomic DNA were included in restriction-digestion with one unit MslI (New England Biolabs, NEB) in 30 μl (1× NEB4 buffer) at 37°C for 1 h and heat inactivated at 80°C for 20 min. Adaptor ligation was conducted with 15 μl of restriction product and 3 μl ligation adapters (Ovation Rapid DR Multiplex System, Nugen Technologies, Leek, Netherlands) in 12 μl of master mix (4.6 μl D1 water, 6 μlL1 ligation buffer mix, and 1.5 μlL3 ligation enzyme) at 25°C for 15 min and heat inactivated at 65°C for 10 min. ..

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  • 94
    New England Biolabs msli
    Msli, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/msli/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    msli - by Bioz Stars, 2021-08
    94/100 stars
      Buy from Supplier

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