bsawi  (New England Biolabs)


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  • 93
    Name:
    BsaWI
    Description:
    BsaWI 250 units
    Catalog Number:
    R0567L
    Price:
    73
    Category:
    Restriction Enzymes
    Size:
    250 units
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    Structured Review

    New England Biolabs bsawi
    BsaWI
    BsaWI 250 units
    https://www.bioz.com/result/bsawi/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bsawi - by Bioz Stars, 2021-04
    93/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Occult Prostate Cancer Effects the Results of Case Control Studies Due to Verification Bias
    Article Snippet: Briefly, DNA was extracted from the prostate tissues using QIAamp DNA Blood Mini Kits (Qiagen, Valencia, CA, USA). .. The MnSOD gene was PCR-amplified and then digested with BsaWI (60°C, 1 hour; New England BioLabs, Beverly, MA, USA). ..

    Article Title: C-type natriuretic peptide (CNP) is a bifurcation factor for sensory neurons
    Article Snippet: The β1-NO-GC ( Gucy1b3 −/−) knock-out mouse, the Npr2 loss of function mutant ( cn / cn ), the lbab strain, and the Thy1-YFP-H reporter line have been described ( , , , ). .. Routine genotyping of CNP lbab mice was carried out by PCR using oligonucleotides P1 (5′-AGCTGGTGGCAATCAGAAAA-3′) and P2 (5′-CTCTTGGGTGCAGAGCTAGG-3′) followed by a Bsa WI (New England BioLabs, Inc.) restriction enzyme digest that results in fragment sizes of 106 and 212 base pairs (wild-type), 106, 212, and 318 base pairs (heterozygous), and 318 base pairs (CNP lbab homozygous), respectively. .. DiI tracing, immunohistochemistry of cryostat sections using antibodies or isolectin IB4, RT-PCR, in situ hybridization, statistical calculations, and patch-clamp recordings were done essentially as described previously ( ). cGMP-levels of E13 DRG after 12.5 min of incubation with or without 0.5 μM CNP-22 (Calbiochem) were determined using the cGMP Biotrak assay from GE Healthcare and was performed according to protocol 4 of the manufacturer product booklet.

    Article Title: Similar Seed Composition Phenotypes Are Observed From CRISPR-Generated In-Frame and Knockout Alleles of a Soybean KASI Ortholog
    Article Snippet: .. For CAPS assays, targeted PCR amplicons were digested with BsaWI (New England Biolabs, Ipswich, MA) overnight at 60°C as per the manufacture's guideline. .. Digested products were run on agarose electrophoresis gels (1.3%).

    Article Title: MnSOD Genotype and Prostate Cancer Risk as a Function of NAT Genotype and Smoking Status
    Article Snippet: DNA was extracted from buccal swabs using QIAamp DNA Blood Mini Kits (Qiagen, Valencia, CA, USA) according to the supplier’s instructions. .. MnSOD was PCR amplified with a primer pair (5′-GTGCTTTCTCGTCTTCAGCA-3′ and 5-AACGCCTCCTGGTACTTCTCC-3′) for 30 thermocycles: 94°C for 30 sec, 55°C for 30 sec, 72°C for 30 sec. PCR products were digested with BsaWI (60°C, 1 h; New England BioLabs, Beverly, MA). .. Digested products were visualized on a 2.5% agarose gel stained with ethidium bromide.

    Mouse Assay:

    Article Title: C-type natriuretic peptide (CNP) is a bifurcation factor for sensory neurons
    Article Snippet: The β1-NO-GC ( Gucy1b3 −/−) knock-out mouse, the Npr2 loss of function mutant ( cn / cn ), the lbab strain, and the Thy1-YFP-H reporter line have been described ( , , , ). .. Routine genotyping of CNP lbab mice was carried out by PCR using oligonucleotides P1 (5′-AGCTGGTGGCAATCAGAAAA-3′) and P2 (5′-CTCTTGGGTGCAGAGCTAGG-3′) followed by a Bsa WI (New England BioLabs, Inc.) restriction enzyme digest that results in fragment sizes of 106 and 212 base pairs (wild-type), 106, 212, and 318 base pairs (heterozygous), and 318 base pairs (CNP lbab homozygous), respectively. .. DiI tracing, immunohistochemistry of cryostat sections using antibodies or isolectin IB4, RT-PCR, in situ hybridization, statistical calculations, and patch-clamp recordings were done essentially as described previously ( ). cGMP-levels of E13 DRG after 12.5 min of incubation with or without 0.5 μM CNP-22 (Calbiochem) were determined using the cGMP Biotrak assay from GE Healthcare and was performed according to protocol 4 of the manufacturer product booklet.

    Concentration Assay:

    Article Title: Programmable DNA looping using engineered bivalent dCas9 complexes
    Article Snippet: Cells were collected by centrifugation, washed twice with 1 mL M9MM, and lysed with 30 μL 25 mg mL−1 lysozyme (Sigma-Aldrich, L6876) at room temperature for 10 min. .. Ten percent SDS was subsequently added to a final concentration of 0.5% and incubated with the lysed cells for further 10 min. Five microliters of this cell lysate were then added to 47 μL of digestion mix (5.3 μL 10x New England Biolab (NEB) Buffer 2, 5.3 μL 10% Triton X-100, and 35 μL MQ water), and incubated at room temperature for further 10 min before 2.4 μL of BsaWI (NEB, 10 units per μL) was added. .. Cell lysate was digested at 60 °C for 1 h. Following digestion, 10% SDS was added to a final concentration of 0.9% and the solution incubated at room temperature for 10 min.

    Incubation:

    Article Title: Programmable DNA looping using engineered bivalent dCas9 complexes
    Article Snippet: Cells were collected by centrifugation, washed twice with 1 mL M9MM, and lysed with 30 μL 25 mg mL−1 lysozyme (Sigma-Aldrich, L6876) at room temperature for 10 min. .. Ten percent SDS was subsequently added to a final concentration of 0.5% and incubated with the lysed cells for further 10 min. Five microliters of this cell lysate were then added to 47 μL of digestion mix (5.3 μL 10x New England Biolab (NEB) Buffer 2, 5.3 μL 10% Triton X-100, and 35 μL MQ water), and incubated at room temperature for further 10 min before 2.4 μL of BsaWI (NEB, 10 units per μL) was added. .. Cell lysate was digested at 60 °C for 1 h. Following digestion, 10% SDS was added to a final concentration of 0.9% and the solution incubated at room temperature for 10 min.

    Amplification:

    Article Title: MnSOD Genotype and Prostate Cancer Risk as a Function of NAT Genotype and Smoking Status
    Article Snippet: DNA was extracted from buccal swabs using QIAamp DNA Blood Mini Kits (Qiagen, Valencia, CA, USA) according to the supplier’s instructions. .. MnSOD was PCR amplified with a primer pair (5′-GTGCTTTCTCGTCTTCAGCA-3′ and 5-AACGCCTCCTGGTACTTCTCC-3′) for 30 thermocycles: 94°C for 30 sec, 55°C for 30 sec, 72°C for 30 sec. PCR products were digested with BsaWI (60°C, 1 h; New England BioLabs, Beverly, MA). .. Digested products were visualized on a 2.5% agarose gel stained with ethidium bromide.

    Size-exclusion Chromatography:

    Article Title: MnSOD Genotype and Prostate Cancer Risk as a Function of NAT Genotype and Smoking Status
    Article Snippet: DNA was extracted from buccal swabs using QIAamp DNA Blood Mini Kits (Qiagen, Valencia, CA, USA) according to the supplier’s instructions. .. MnSOD was PCR amplified with a primer pair (5′-GTGCTTTCTCGTCTTCAGCA-3′ and 5-AACGCCTCCTGGTACTTCTCC-3′) for 30 thermocycles: 94°C for 30 sec, 55°C for 30 sec, 72°C for 30 sec. PCR products were digested with BsaWI (60°C, 1 h; New England BioLabs, Beverly, MA). .. Digested products were visualized on a 2.5% agarose gel stained with ethidium bromide.

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  • 93
    New England Biolabs bsa wi
    <t>Bsa</t> WI digestion of genomic <t>amplicons</t> of CsPPR336 from 401734-3A (P3A; Psm −/−), inbred B ( Psm + / +), and an artificial heterozygote (AH) from mixture of equal amounts of DNAs from P3A and inbred B
    Bsa Wi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bsa wi/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bsa wi - by Bioz Stars, 2021-04
    93/100 stars
      Buy from Supplier

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    Bsa WI digestion of genomic amplicons of CsPPR336 from 401734-3A (P3A; Psm −/−), inbred B ( Psm + / +), and an artificial heterozygote (AH) from mixture of equal amounts of DNAs from P3A and inbred B

    Journal: TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik

    Article Title: Pentatricopeptide repeat 336 as the candidate gene for paternal sorting of mitochondria (Psm) in cucumber

    doi: 10.1007/s00122-016-2751-x

    Figure Lengend Snippet: Bsa WI digestion of genomic amplicons of CsPPR336 from 401734-3A (P3A; Psm −/−), inbred B ( Psm + / +), and an artificial heterozygote (AH) from mixture of equal amounts of DNAs from P3A and inbred B

    Article Snippet: Amplicons were digested by Bsa WI followed the manufacturer’s (New England Bio Labs) instructions and reactions were size fractioned through 1 %-agarose gels.

    Techniques: