bsa wi  (New England Biolabs)


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  • 94
    Name:
    BsaWI
    Description:
    BsaWI 250 units
    Catalog Number:
    r0567l
    Price:
    73
    Size:
    250 units
    Category:
    Restriction Enzymes
    Buy from Supplier


    Structured Review

    New England Biolabs bsa wi
    BsaWI
    BsaWI 250 units
    https://www.bioz.com/result/bsa wi/product/New England Biolabs
    Average 94 stars, based on 204 article reviews
    Price from $9.99 to $1999.99
    bsa wi - by Bioz Stars, 2020-09
    94/100 stars

    Images

    1) Product Images from "Pentatricopeptide repeat 336 as the candidate gene for paternal sorting of mitochondria (Psm) in cucumber"

    Article Title: Pentatricopeptide repeat 336 as the candidate gene for paternal sorting of mitochondria (Psm) in cucumber

    Journal: TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik

    doi: 10.1007/s00122-016-2751-x

    Bsa WI digestion of genomic amplicons of CsPPR336 from 401734-3A (P3A; Psm −/−), inbred B ( Psm + / +), and an artificial heterozygote (AH) from mixture of equal amounts of DNAs from P3A and inbred B
    Figure Legend Snippet: Bsa WI digestion of genomic amplicons of CsPPR336 from 401734-3A (P3A; Psm −/−), inbred B ( Psm + / +), and an artificial heterozygote (AH) from mixture of equal amounts of DNAs from P3A and inbred B

    Techniques Used:

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Molecular Evidence of Genome Editing in a Mouse Model of Immunodeficiency
    Article Snippet: .. The PCR products were digested overnight with Bsa WI (NEB, UK) and separated on a 0.7% agarose gel. .. The DNA was transferred overnight onto GeneScreen Plus Membrane (Perkin Elmer, USA), hybridised overnight using as probe [α-32P]-ATP labeled Prkdc template, washed and exposed to a phosphoimager screen.

    Article Title: Localization of sequence variations in PGC-1? influence their modifying effect in Huntington disease
    Article Snippet: .. The respective PCR products were incubated with 4U MspI (rs8192678) and 1U BsaWI (rs2970847) according to the manufacturer's instructions (New England Biolabs, Inc., Beverly, MA, USA). .. Single correct typing results as well as ambiguous results were verified and controlled by sequencing with CEQ 8000 Dye Terminator Cycler Sequencing (Beckman Coulter Inc., USA) according to manufacturer's instructions.

    Article Title: Similar Seed Composition Phenotypes Are Observed From CRISPR-Generated In-Frame and Knockout Alleles of a Soybean KASI Ortholog
    Article Snippet: .. For CAPS assays, targeted PCR amplicons were digested with BsaWI (New England Biolabs, Ipswich, MA) overnight at 60°C as per the manufacture's guideline. .. Digested products were run on agarose electrophoresis gels (1.3%).

    Article Title: Molecular Evidence of Genome Editing in a Mouse Model of Immunodeficiency
    Article Snippet: .. The PCR products were digested overnight with Bsa WI (NEB, UK) and separated on a 0.7% agarose gel. .. The DNA was transferred overnight onto GeneScreen Plus Membrane (Perkin Elmer, USA), hybridised overnight using as probe [α-32P]-ATP labeled Prkdc template, washed and exposed to a phosphoimager screen.

    Article Title: MnSOD Genotype and Prostate Cancer Risk as a Function of NAT Genotype and Smoking Status
    Article Snippet: .. MnSOD was PCR amplified with a primer pair (5′-GTGCTTTCTCGTCTTCAGCA-3′ and 5-AACGCCTCCTGGTACTTCTCC-3′) for 30 thermocycles: 94°C for 30 sec, 55°C for 30 sec, 72°C for 30 sec. PCR products were digested with BsaWI (60°C, 1 h; New England BioLabs, Beverly, MA). .. Digested products were visualized on a 2.5% agarose gel stained with ethidium bromide.

    Incubation:

    Article Title: Localization of sequence variations in PGC-1? influence their modifying effect in Huntington disease
    Article Snippet: .. The respective PCR products were incubated with 4U MspI (rs8192678) and 1U BsaWI (rs2970847) according to the manufacturer's instructions (New England Biolabs, Inc., Beverly, MA, USA). .. Single correct typing results as well as ambiguous results were verified and controlled by sequencing with CEQ 8000 Dye Terminator Cycler Sequencing (Beckman Coulter Inc., USA) according to manufacturer's instructions.

    Amplification:

    Article Title: MnSOD Genotype and Prostate Cancer Risk as a Function of NAT Genotype and Smoking Status
    Article Snippet: .. MnSOD was PCR amplified with a primer pair (5′-GTGCTTTCTCGTCTTCAGCA-3′ and 5-AACGCCTCCTGGTACTTCTCC-3′) for 30 thermocycles: 94°C for 30 sec, 55°C for 30 sec, 72°C for 30 sec. PCR products were digested with BsaWI (60°C, 1 h; New England BioLabs, Beverly, MA). .. Digested products were visualized on a 2.5% agarose gel stained with ethidium bromide.

    Agarose Gel Electrophoresis:

    Article Title: Molecular Evidence of Genome Editing in a Mouse Model of Immunodeficiency
    Article Snippet: .. The PCR products were digested overnight with Bsa WI (NEB, UK) and separated on a 0.7% agarose gel. .. The DNA was transferred overnight onto GeneScreen Plus Membrane (Perkin Elmer, USA), hybridised overnight using as probe [α-32P]-ATP labeled Prkdc template, washed and exposed to a phosphoimager screen.

    Article Title: Molecular Evidence of Genome Editing in a Mouse Model of Immunodeficiency
    Article Snippet: .. The PCR products were digested overnight with Bsa WI (NEB, UK) and separated on a 0.7% agarose gel. .. The DNA was transferred overnight onto GeneScreen Plus Membrane (Perkin Elmer, USA), hybridised overnight using as probe [α-32P]-ATP labeled Prkdc template, washed and exposed to a phosphoimager screen.

    Size-exclusion Chromatography:

    Article Title: MnSOD Genotype and Prostate Cancer Risk as a Function of NAT Genotype and Smoking Status
    Article Snippet: .. MnSOD was PCR amplified with a primer pair (5′-GTGCTTTCTCGTCTTCAGCA-3′ and 5-AACGCCTCCTGGTACTTCTCC-3′) for 30 thermocycles: 94°C for 30 sec, 55°C for 30 sec, 72°C for 30 sec. PCR products were digested with BsaWI (60°C, 1 h; New England BioLabs, Beverly, MA). .. Digested products were visualized on a 2.5% agarose gel stained with ethidium bromide.

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  • 96
    New England Biolabs bsa wi
    <t>Bsa</t> WI digestion of genomic <t>amplicons</t> of CsPPR336 from 401734-3A (P3A; Psm −/−), inbred B ( Psm + / +), and an artificial heterozygote (AH) from mixture of equal amounts of DNAs from P3A and inbred B
    Bsa Wi, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 7 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bsa wi/product/New England Biolabs
    Average 96 stars, based on 7 article reviews
    Price from $9.99 to $1999.99
    bsa wi - by Bioz Stars, 2020-09
    96/100 stars
      Buy from Supplier

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    Bsa WI digestion of genomic amplicons of CsPPR336 from 401734-3A (P3A; Psm −/−), inbred B ( Psm + / +), and an artificial heterozygote (AH) from mixture of equal amounts of DNAs from P3A and inbred B

    Journal: TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik

    Article Title: Pentatricopeptide repeat 336 as the candidate gene for paternal sorting of mitochondria (Psm) in cucumber

    doi: 10.1007/s00122-016-2751-x

    Figure Lengend Snippet: Bsa WI digestion of genomic amplicons of CsPPR336 from 401734-3A (P3A; Psm −/−), inbred B ( Psm + / +), and an artificial heterozygote (AH) from mixture of equal amounts of DNAs from P3A and inbred B

    Article Snippet: Amplicons were digested by Bsa WI followed the manufacturer’s (New England Bio Labs) instructions and reactions were size fractioned through 1 %-agarose gels.

    Techniques: