Ngomiv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 18 article reviews
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1) Product Images from "Insertion of Exogenous Genes within the ORF1a Coding Region of Porcine Astrovirus"
Article Title: Insertion of Exogenous Genes within the ORF1a Coding Region of Porcine Astrovirus
Figure Legend Snippet: ( a ) Strategy for the construction of a full-length DNA-launched infectious clone of PAstV. The T7 promoter of pMD123 was replaced with a cytomegalovirus (CMV) promotor using SpeI and NgoMIV . The hepatitis delta virus ribozyme was inserted into the 3′ terminal end of the PAstV-GX1 genome with XhoI and SacII. The restriction enzyme sites above the full-length cDNA clones are indicated by arrows. ( b ) Cytopathic effects were observed in PK-15 cells infected with rescued viruses (magnification 10×). ( c ) IFA analysis of the Cap protein expression in PK-15 cells. PK-15 cells infected with rescued and parental viruses were fixed at approximately 48 h.p.i and subjected to immunofluorescence using a polyclonal antibody against capsid protein. The PK-15 cells were stained with goat anti-mouse IgG H L (CoraLite488) (magnification 20×). ( d ) Growth curves of the recombinant and parental viruses. PK-15 cells were infected with recovered and parental viruses at a multiplicity of infection of 0.01. The viral titers were determined as TCID 50 .
Techniques Used: Clone Assay, Infection, Immunofluorescence, Expressing, Staining, Recombinant