ngomiv  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 98
    Name:
    NgoMIV
    Description:
    NgoMIV 5 000 units
    Catalog Number:
    R0564L
    Price:
    269
    Category:
    Restriction Enzymes
    Size:
    5 000 units
    Buy from Supplier


    Structured Review

    New England Biolabs ngomiv
    NgoMIV
    NgoMIV 5 000 units
    https://www.bioz.com/result/ngomiv/product/New England Biolabs
    Average 98 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ngomiv - by Bioz Stars, 2021-05
    98/100 stars

    Images

    Related Articles

    Clone Assay:

    Article Title: The Use of a Chimeric Rhodopsin Vector for the Detection of New Proteorhodopsins Based on Color
    Article Snippet: Cloning and Expression PCR products were double-digested with Kpn I and NgoM IV in NEBufferTM 1.1 buffer (New England BioLabs) for 2 h at 37°C, cleaned using ½ volume of phenol and ½ volume of chloroform then 1 volume of chloroform, and heated to 70°C for 10 min to remove residual chloroform by evaporation. .. The cloning vector was extracted from cells using only QIAprep Spin Miniprep Kit (Qiagen) since other methods do not enable efficient restriction, digested with Kpn I and NgoM IV in 1.1 buffer (New England BioLabs), and separated from the 114 bp insert on 1% agarose gel. .. Cut vector was extracted from gel using NucleoSpin® Gel and PCR Clean-up (Macherey-Nagel, Düren, Germany).

    Article Title: The use of a chimeric rhodopsin vector for the detection of new proteorhodopsins based on color
    Article Snippet: Polymerase chain reaction amplification was carried out in a total volume of 50 μ l containing 1 μ l DNA template, 0.2 mM dNTPs, 1X Ex Taq buffer (Mg2+ plus), 0.6 μ M primers (each) and 125 U Takara Ex Taq™ DNA polymerase. .. Cloning and expression PCR products were double-digested with Kpn I and NgoM IV in NE Buffer™ 1.1 buffer (New England BioLabs) for 2 hours at 37°C, cleaned using ½ volume of phenol and ½ volume of chloroform then 1 volume of chloroform, and heated to 70°C for 10 min to remove residual chloroform by evaporation. .. The cloning vector was extracted from cells using only QIAprep Spin Miniprep Kit (Quiagen) since other methods do not enable efficient restriction, digested with Kpn I and NgoM IV in 1.1 buffer (New England BioLabs), and separated from the 114 bp insert on 1% agarose gel.

    Article Title: Phenotypic co-receptor tropism and Maraviroc sensitivity in HIV-1 subtype C from East Africa
    Article Snippet: Plasmids pNL4-3 and pMJ4 were also amplified with 6435F and 8329R primers. .. The resultant fragments were gel purified using QIAquickGel Extraction Kit (Qiagen, USA) and cloned into a pMN-K7-Luc-IRESs-NefΔgp120 plasmid following digestion with NgoMIV and MluI-HF (New England Biolabs, US) and ligation with T4 DNA ligase (New England Biolabs, US). .. The recombinant viruses were produced transfecting the plasmids using FuGENE® HD Transfection Reagent (Promega, US) in 293 T cell line.

    Plasmid Preparation:

    Article Title: The Use of a Chimeric Rhodopsin Vector for the Detection of New Proteorhodopsins Based on Color
    Article Snippet: Cloning and Expression PCR products were double-digested with Kpn I and NgoM IV in NEBufferTM 1.1 buffer (New England BioLabs) for 2 h at 37°C, cleaned using ½ volume of phenol and ½ volume of chloroform then 1 volume of chloroform, and heated to 70°C for 10 min to remove residual chloroform by evaporation. .. The cloning vector was extracted from cells using only QIAprep Spin Miniprep Kit (Qiagen) since other methods do not enable efficient restriction, digested with Kpn I and NgoM IV in 1.1 buffer (New England BioLabs), and separated from the 114 bp insert on 1% agarose gel. .. Cut vector was extracted from gel using NucleoSpin® Gel and PCR Clean-up (Macherey-Nagel, Düren, Germany).

    Article Title: Phenotypic co-receptor tropism and Maraviroc sensitivity in HIV-1 subtype C from East Africa
    Article Snippet: Plasmids pNL4-3 and pMJ4 were also amplified with 6435F and 8329R primers. .. The resultant fragments were gel purified using QIAquickGel Extraction Kit (Qiagen, USA) and cloned into a pMN-K7-Luc-IRESs-NefΔgp120 plasmid following digestion with NgoMIV and MluI-HF (New England Biolabs, US) and ligation with T4 DNA ligase (New England Biolabs, US). .. The recombinant viruses were produced transfecting the plasmids using FuGENE® HD Transfection Reagent (Promega, US) in 293 T cell line.

    Agarose Gel Electrophoresis:

    Article Title: The Use of a Chimeric Rhodopsin Vector for the Detection of New Proteorhodopsins Based on Color
    Article Snippet: Cloning and Expression PCR products were double-digested with Kpn I and NgoM IV in NEBufferTM 1.1 buffer (New England BioLabs) for 2 h at 37°C, cleaned using ½ volume of phenol and ½ volume of chloroform then 1 volume of chloroform, and heated to 70°C for 10 min to remove residual chloroform by evaporation. .. The cloning vector was extracted from cells using only QIAprep Spin Miniprep Kit (Qiagen) since other methods do not enable efficient restriction, digested with Kpn I and NgoM IV in 1.1 buffer (New England BioLabs), and separated from the 114 bp insert on 1% agarose gel. .. Cut vector was extracted from gel using NucleoSpin® Gel and PCR Clean-up (Macherey-Nagel, Düren, Germany).

    Expressing:

    Article Title: The use of a chimeric rhodopsin vector for the detection of new proteorhodopsins based on color
    Article Snippet: Polymerase chain reaction amplification was carried out in a total volume of 50 μ l containing 1 μ l DNA template, 0.2 mM dNTPs, 1X Ex Taq buffer (Mg2+ plus), 0.6 μ M primers (each) and 125 U Takara Ex Taq™ DNA polymerase. .. Cloning and expression PCR products were double-digested with Kpn I and NgoM IV in NE Buffer™ 1.1 buffer (New England BioLabs) for 2 hours at 37°C, cleaned using ½ volume of phenol and ½ volume of chloroform then 1 volume of chloroform, and heated to 70°C for 10 min to remove residual chloroform by evaporation. .. The cloning vector was extracted from cells using only QIAprep Spin Miniprep Kit (Quiagen) since other methods do not enable efficient restriction, digested with Kpn I and NgoM IV in 1.1 buffer (New England BioLabs), and separated from the 114 bp insert on 1% agarose gel.

    Polymerase Chain Reaction:

    Article Title: The use of a chimeric rhodopsin vector for the detection of new proteorhodopsins based on color
    Article Snippet: Polymerase chain reaction amplification was carried out in a total volume of 50 μ l containing 1 μ l DNA template, 0.2 mM dNTPs, 1X Ex Taq buffer (Mg2+ plus), 0.6 μ M primers (each) and 125 U Takara Ex Taq™ DNA polymerase. .. Cloning and expression PCR products were double-digested with Kpn I and NgoM IV in NE Buffer™ 1.1 buffer (New England BioLabs) for 2 hours at 37°C, cleaned using ½ volume of phenol and ½ volume of chloroform then 1 volume of chloroform, and heated to 70°C for 10 min to remove residual chloroform by evaporation. .. The cloning vector was extracted from cells using only QIAprep Spin Miniprep Kit (Quiagen) since other methods do not enable efficient restriction, digested with Kpn I and NgoM IV in 1.1 buffer (New England BioLabs), and separated from the 114 bp insert on 1% agarose gel.

    Article Title: Allele, Genotype and Haplotype Structures of Functional Polymorphic Variants in Endothelial Nitric Oxide Synthase (eNOS), Angiotensinogen (ACE) and Aldosterone Synthase (CYP11B2) Genes in Healthy Pregnant Women of Indian Ethnicity
    Article Snippet: After restriction digestion with MboI (New England Biolabs), bands were observed at 119 and 87 bp for homozygous variant type, 206, 119 and 87 bp for heterozygous variant type and a single band at 206 bp for normal type on 8% polyacrylamide gel. .. Similarly, the amplified PCR product of eNOS −786T > C polymorphism resulted in a 223 bp amplicon and after restriction digestion with NgoMIV (New England Biolabs), bands were observed at 115 and 108 bp for homozygous variant, 223, 115 and 108 bp for heterozygous variant type and a single band at 223 bp for normal type on 12% polyacrylamide gel. .. On the other hand, the amplified PCR products of the eNOS 27 bp direct repeat on intron 4 VNTR region were directly resolved on 7% low melting agarose, and the fragments were visualized by ethidium bromide staining and ultraviolet transillumination.

    Article Title: Genetic polymorphism of the Nrf2 promoter region is associated with vitiligo risk in Han Chinese populations
    Article Snippet: DNA purity and concentration were evaluated by spectrophotometric measurements of absorbance at 260 and 280 nm. .. The PCR‐RFLP method was used to amplify Nrf2 (rs35652124, rs6721961) and HO‐1 (rs2071746) with the following forward and reverse primers: Nrf2 rs35652124 T/C, 5′‐CCT TGC CCT GCT TTT ATC TC‐3′ and 5′‐CTT CTC CGT TTG CCT TTG AC‐3′; Nrf2 rs6721961 G/T, 5′‐GAA AGG CGT TGG TGT AGG AG‐3′ and 5′‐GAA TGG AGA CAC GTG GGA GT‐3′; HO‐1 rs2071746 A/T, 5′‐GTT CCT GAT GTT GCC CAC CAA GC‐3′ and 5′‐CTG CAG GCT CTG GGT GTG ATT TTG‐3′, which generated products of 264, 278, and 151 bp, respectively, that were digested with Bse RI (MBI Fermentas, Leon‐Rot, Germany), Ngo MIV and Hind III (both from New England Biolabs, Ipswich, MA, USA) restriction enzymes respectively. .. The fragments that were generated by the digestion were 192 and 72 bp for Nrf2 rs35652124 T/C (Fig. C), 215 and 63 bp for Nrf2 rs6721961 (Fig. C), and 20 and 131 bp for HO‐1 rs2071746 (Fig. F).

    Evaporation:

    Article Title: The use of a chimeric rhodopsin vector for the detection of new proteorhodopsins based on color
    Article Snippet: Polymerase chain reaction amplification was carried out in a total volume of 50 μ l containing 1 μ l DNA template, 0.2 mM dNTPs, 1X Ex Taq buffer (Mg2+ plus), 0.6 μ M primers (each) and 125 U Takara Ex Taq™ DNA polymerase. .. Cloning and expression PCR products were double-digested with Kpn I and NgoM IV in NE Buffer™ 1.1 buffer (New England BioLabs) for 2 hours at 37°C, cleaned using ½ volume of phenol and ½ volume of chloroform then 1 volume of chloroform, and heated to 70°C for 10 min to remove residual chloroform by evaporation. .. The cloning vector was extracted from cells using only QIAprep Spin Miniprep Kit (Quiagen) since other methods do not enable efficient restriction, digested with Kpn I and NgoM IV in 1.1 buffer (New England BioLabs), and separated from the 114 bp insert on 1% agarose gel.

    Amplification:

    Article Title: Allele, Genotype and Haplotype Structures of Functional Polymorphic Variants in Endothelial Nitric Oxide Synthase (eNOS), Angiotensinogen (ACE) and Aldosterone Synthase (CYP11B2) Genes in Healthy Pregnant Women of Indian Ethnicity
    Article Snippet: After restriction digestion with MboI (New England Biolabs), bands were observed at 119 and 87 bp for homozygous variant type, 206, 119 and 87 bp for heterozygous variant type and a single band at 206 bp for normal type on 8% polyacrylamide gel. .. Similarly, the amplified PCR product of eNOS −786T > C polymorphism resulted in a 223 bp amplicon and after restriction digestion with NgoMIV (New England Biolabs), bands were observed at 115 and 108 bp for homozygous variant, 223, 115 and 108 bp for heterozygous variant type and a single band at 223 bp for normal type on 12% polyacrylamide gel. .. On the other hand, the amplified PCR products of the eNOS 27 bp direct repeat on intron 4 VNTR region were directly resolved on 7% low melting agarose, and the fragments were visualized by ethidium bromide staining and ultraviolet transillumination.

    Variant Assay:

    Article Title: Allele, Genotype and Haplotype Structures of Functional Polymorphic Variants in Endothelial Nitric Oxide Synthase (eNOS), Angiotensinogen (ACE) and Aldosterone Synthase (CYP11B2) Genes in Healthy Pregnant Women of Indian Ethnicity
    Article Snippet: After restriction digestion with MboI (New England Biolabs), bands were observed at 119 and 87 bp for homozygous variant type, 206, 119 and 87 bp for heterozygous variant type and a single band at 206 bp for normal type on 8% polyacrylamide gel. .. Similarly, the amplified PCR product of eNOS −786T > C polymorphism resulted in a 223 bp amplicon and after restriction digestion with NgoMIV (New England Biolabs), bands were observed at 115 and 108 bp for homozygous variant, 223, 115 and 108 bp for heterozygous variant type and a single band at 223 bp for normal type on 12% polyacrylamide gel. .. On the other hand, the amplified PCR products of the eNOS 27 bp direct repeat on intron 4 VNTR region were directly resolved on 7% low melting agarose, and the fragments were visualized by ethidium bromide staining and ultraviolet transillumination.

    Purification:

    Article Title: Phenotypic co-receptor tropism and Maraviroc sensitivity in HIV-1 subtype C from East Africa
    Article Snippet: Plasmids pNL4-3 and pMJ4 were also amplified with 6435F and 8329R primers. .. The resultant fragments were gel purified using QIAquickGel Extraction Kit (Qiagen, USA) and cloned into a pMN-K7-Luc-IRESs-NefΔgp120 plasmid following digestion with NgoMIV and MluI-HF (New England Biolabs, US) and ligation with T4 DNA ligase (New England Biolabs, US). .. The recombinant viruses were produced transfecting the plasmids using FuGENE® HD Transfection Reagent (Promega, US) in 293 T cell line.

    Ligation:

    Article Title: Phenotypic co-receptor tropism and Maraviroc sensitivity in HIV-1 subtype C from East Africa
    Article Snippet: Plasmids pNL4-3 and pMJ4 were also amplified with 6435F and 8329R primers. .. The resultant fragments were gel purified using QIAquickGel Extraction Kit (Qiagen, USA) and cloned into a pMN-K7-Luc-IRESs-NefΔgp120 plasmid following digestion with NgoMIV and MluI-HF (New England Biolabs, US) and ligation with T4 DNA ligase (New England Biolabs, US). .. The recombinant viruses were produced transfecting the plasmids using FuGENE® HD Transfection Reagent (Promega, US) in 293 T cell line.

    In Vitro:

    Article Title: An ex vivo avian leukocyte culture model for West Nile virus infection
    Article Snippet: NY99 wild-type and mutant viral stocks were derived from infectious clones as previously described ( ). .. Briefly, the 5′ and 3′ plasmids were digested with Ngo MIV, ligated, and linearized with Xba I (New England Biolabs) before in vitro transcription with the Ampliscribe High-Yield T7 Transcription kit (Epicentre Biotechnologies). .. Viral RNA was transfected into BHK-21 cells by electroporation ( ).

    Cellular Antioxidant Activity Assay:

    Article Title: Genetic polymorphism of the Nrf2 promoter region is associated with vitiligo risk in Han Chinese populations
    Article Snippet: DNA purity and concentration were evaluated by spectrophotometric measurements of absorbance at 260 and 280 nm. .. The PCR‐RFLP method was used to amplify Nrf2 (rs35652124, rs6721961) and HO‐1 (rs2071746) with the following forward and reverse primers: Nrf2 rs35652124 T/C, 5′‐CCT TGC CCT GCT TTT ATC TC‐3′ and 5′‐CTT CTC CGT TTG CCT TTG AC‐3′; Nrf2 rs6721961 G/T, 5′‐GAA AGG CGT TGG TGT AGG AG‐3′ and 5′‐GAA TGG AGA CAC GTG GGA GT‐3′; HO‐1 rs2071746 A/T, 5′‐GTT CCT GAT GTT GCC CAC CAA GC‐3′ and 5′‐CTG CAG GCT CTG GGT GTG ATT TTG‐3′, which generated products of 264, 278, and 151 bp, respectively, that were digested with Bse RI (MBI Fermentas, Leon‐Rot, Germany), Ngo MIV and Hind III (both from New England Biolabs, Ipswich, MA, USA) restriction enzymes respectively. .. The fragments that were generated by the digestion were 192 and 72 bp for Nrf2 rs35652124 T/C (Fig. C), 215 and 63 bp for Nrf2 rs6721961 (Fig. C), and 20 and 131 bp for HO‐1 rs2071746 (Fig. F).

    CTG Assay:

    Article Title: Genetic polymorphism of the Nrf2 promoter region is associated with vitiligo risk in Han Chinese populations
    Article Snippet: DNA purity and concentration were evaluated by spectrophotometric measurements of absorbance at 260 and 280 nm. .. The PCR‐RFLP method was used to amplify Nrf2 (rs35652124, rs6721961) and HO‐1 (rs2071746) with the following forward and reverse primers: Nrf2 rs35652124 T/C, 5′‐CCT TGC CCT GCT TTT ATC TC‐3′ and 5′‐CTT CTC CGT TTG CCT TTG AC‐3′; Nrf2 rs6721961 G/T, 5′‐GAA AGG CGT TGG TGT AGG AG‐3′ and 5′‐GAA TGG AGA CAC GTG GGA GT‐3′; HO‐1 rs2071746 A/T, 5′‐GTT CCT GAT GTT GCC CAC CAA GC‐3′ and 5′‐CTG CAG GCT CTG GGT GTG ATT TTG‐3′, which generated products of 264, 278, and 151 bp, respectively, that were digested with Bse RI (MBI Fermentas, Leon‐Rot, Germany), Ngo MIV and Hind III (both from New England Biolabs, Ipswich, MA, USA) restriction enzymes respectively. .. The fragments that were generated by the digestion were 192 and 72 bp for Nrf2 rs35652124 T/C (Fig. C), 215 and 63 bp for Nrf2 rs6721961 (Fig. C), and 20 and 131 bp for HO‐1 rs2071746 (Fig. F).

    Generated:

    Article Title: Genetic polymorphism of the Nrf2 promoter region is associated with vitiligo risk in Han Chinese populations
    Article Snippet: DNA purity and concentration were evaluated by spectrophotometric measurements of absorbance at 260 and 280 nm. .. The PCR‐RFLP method was used to amplify Nrf2 (rs35652124, rs6721961) and HO‐1 (rs2071746) with the following forward and reverse primers: Nrf2 rs35652124 T/C, 5′‐CCT TGC CCT GCT TTT ATC TC‐3′ and 5′‐CTT CTC CGT TTG CCT TTG AC‐3′; Nrf2 rs6721961 G/T, 5′‐GAA AGG CGT TGG TGT AGG AG‐3′ and 5′‐GAA TGG AGA CAC GTG GGA GT‐3′; HO‐1 rs2071746 A/T, 5′‐GTT CCT GAT GTT GCC CAC CAA GC‐3′ and 5′‐CTG CAG GCT CTG GGT GTG ATT TTG‐3′, which generated products of 264, 278, and 151 bp, respectively, that were digested with Bse RI (MBI Fermentas, Leon‐Rot, Germany), Ngo MIV and Hind III (both from New England Biolabs, Ipswich, MA, USA) restriction enzymes respectively. .. The fragments that were generated by the digestion were 192 and 72 bp for Nrf2 rs35652124 T/C (Fig. C), 215 and 63 bp for Nrf2 rs6721961 (Fig. C), and 20 and 131 bp for HO‐1 rs2071746 (Fig. F).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    New England Biolabs restriction enzymes ngomiv
    Lysates of N. gonorrhoeae fragments pECFP-N1 and damage DNA from VK2/E6E7 cells. A. DNA agarose gel showing the digestion of pECFP-N1 plasmid by HindIII (positive control, lane 2), MS11 P+ lysate (lane 3), and MS11 P+ HI lysate (lane 5). Lane 5 shows bacterial MS11 P+ lysate without pECFP-N1 and lane 1 shows uncut circular pECFP-N1. B. PFGE analysis of purified VK2/E6E7 genomic DNA treated for 24 h with: lane 1: PBS (negative control), lane 2: MS11 P+ lysate, lane 3: MS11 P+ HI lysate. Lane 4 shows bacterial MS11 P+ lysate without VK2/E6E7 genomic DNA. C. Graph showing quantification of DNA smears (measured directly underneath and below the band). Shown are smear pixel intensities of cellular DNA alone and cellular DNA exposed to bacterial lysates and HI bacterial lysates. D. PFGE showing genomic DNA subjected to commercial restriction enzymes for 24 h. Lane 1: DNA incubated with CutSmart reaction buffer (negative control). Lane 2: DNA incubated with <t>NgoMIV.</t> Lane 3: DNA incubated with MfeI, Lane 4: DNA incubated with NgoMIV and MfeI Lane 5: DNA incubated with NgoMIV and <t>BamHI/KpnI/MfeI</t> (BKM).
    Restriction Enzymes Ngomiv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction enzymes ngomiv/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    restriction enzymes ngomiv - by Bioz Stars, 2021-05
    93/100 stars
      Buy from Supplier

    Image Search Results


    Lysates of N. gonorrhoeae fragments pECFP-N1 and damage DNA from VK2/E6E7 cells. A. DNA agarose gel showing the digestion of pECFP-N1 plasmid by HindIII (positive control, lane 2), MS11 P+ lysate (lane 3), and MS11 P+ HI lysate (lane 5). Lane 5 shows bacterial MS11 P+ lysate without pECFP-N1 and lane 1 shows uncut circular pECFP-N1. B. PFGE analysis of purified VK2/E6E7 genomic DNA treated for 24 h with: lane 1: PBS (negative control), lane 2: MS11 P+ lysate, lane 3: MS11 P+ HI lysate. Lane 4 shows bacterial MS11 P+ lysate without VK2/E6E7 genomic DNA. C. Graph showing quantification of DNA smears (measured directly underneath and below the band). Shown are smear pixel intensities of cellular DNA alone and cellular DNA exposed to bacterial lysates and HI bacterial lysates. D. PFGE showing genomic DNA subjected to commercial restriction enzymes for 24 h. Lane 1: DNA incubated with CutSmart reaction buffer (negative control). Lane 2: DNA incubated with NgoMIV. Lane 3: DNA incubated with MfeI, Lane 4: DNA incubated with NgoMIV and MfeI Lane 5: DNA incubated with NgoMIV and BamHI/KpnI/MfeI (BKM).

    Journal: PLoS ONE

    Article Title: Restriction Endonucleases from Invasive Neisseria gonorrhoeae Cause Double-Strand Breaks and Distort Mitosis in Epithelial Cells during Infection

    doi: 10.1371/journal.pone.0114208

    Figure Lengend Snippet: Lysates of N. gonorrhoeae fragments pECFP-N1 and damage DNA from VK2/E6E7 cells. A. DNA agarose gel showing the digestion of pECFP-N1 plasmid by HindIII (positive control, lane 2), MS11 P+ lysate (lane 3), and MS11 P+ HI lysate (lane 5). Lane 5 shows bacterial MS11 P+ lysate without pECFP-N1 and lane 1 shows uncut circular pECFP-N1. B. PFGE analysis of purified VK2/E6E7 genomic DNA treated for 24 h with: lane 1: PBS (negative control), lane 2: MS11 P+ lysate, lane 3: MS11 P+ HI lysate. Lane 4 shows bacterial MS11 P+ lysate without VK2/E6E7 genomic DNA. C. Graph showing quantification of DNA smears (measured directly underneath and below the band). Shown are smear pixel intensities of cellular DNA alone and cellular DNA exposed to bacterial lysates and HI bacterial lysates. D. PFGE showing genomic DNA subjected to commercial restriction enzymes for 24 h. Lane 1: DNA incubated with CutSmart reaction buffer (negative control). Lane 2: DNA incubated with NgoMIV. Lane 3: DNA incubated with MfeI, Lane 4: DNA incubated with NgoMIV and MfeI Lane 5: DNA incubated with NgoMIV and BamHI/KpnI/MfeI (BKM).

    Article Snippet: As positive controls, 0.5 µl each of the restriction enzymes NgoMIV, BamHI, KpnI and MfeI (New England Biolabs, Ipswich, MA, USA) and 5 µl CutSmart buffer were added to 1 µg genomic DNA in 50 µl reactions.

    Techniques: Agarose Gel Electrophoresis, Plasmid Preparation, Positive Control, Purification, Negative Control, Incubation