ngomiv  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93

    Structured Review

    New England Biolabs ngomiv
    ( a ) Strategy for the construction of a full-length DNA-launched infectious clone of PAstV. The T7 promoter of pMD123 was replaced with a cytomegalovirus (CMV) promotor using <t>SpeI</t> and <t>NgoMIV</t> . The hepatitis delta virus ribozyme was inserted into the 3′ terminal end of the PAstV-GX1 genome with XhoI and SacII. The restriction enzyme sites above the full-length cDNA clones are indicated by arrows. ( b ) Cytopathic effects were observed in PK-15 cells infected with rescued viruses (magnification 10×). ( c ) IFA analysis of the Cap protein expression in PK-15 cells. PK-15 cells infected with rescued and parental viruses were fixed at approximately 48 h.p.i and subjected to immunofluorescence using a polyclonal antibody against capsid protein. The PK-15 cells were stained with goat anti-mouse IgG H L (CoraLite488) (magnification 20×). ( d ) Growth curves of the recombinant and parental viruses. PK-15 cells were infected with recovered and parental viruses at a multiplicity of infection of 0.01. The viral titers were determined as TCID 50 .
    Ngomiv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ngomiv/product/New England Biolabs
    Average 93 stars, based on 18 article reviews
    Price from $9.99 to $1999.99
    ngomiv - by Bioz Stars, 2022-09
    93/100 stars

    Images

    1) Product Images from "Insertion of Exogenous Genes within the ORF1a Coding Region of Porcine Astrovirus"

    Article Title: Insertion of Exogenous Genes within the ORF1a Coding Region of Porcine Astrovirus

    Journal: Viruses

    doi: 10.3390/v13112119

    ( a ) Strategy for the construction of a full-length DNA-launched infectious clone of PAstV. The T7 promoter of pMD123 was replaced with a cytomegalovirus (CMV) promotor using SpeI and NgoMIV . The hepatitis delta virus ribozyme was inserted into the 3′ terminal end of the PAstV-GX1 genome with XhoI and SacII. The restriction enzyme sites above the full-length cDNA clones are indicated by arrows. ( b ) Cytopathic effects were observed in PK-15 cells infected with rescued viruses (magnification 10×). ( c ) IFA analysis of the Cap protein expression in PK-15 cells. PK-15 cells infected with rescued and parental viruses were fixed at approximately 48 h.p.i and subjected to immunofluorescence using a polyclonal antibody against capsid protein. The PK-15 cells were stained with goat anti-mouse IgG H L (CoraLite488) (magnification 20×). ( d ) Growth curves of the recombinant and parental viruses. PK-15 cells were infected with recovered and parental viruses at a multiplicity of infection of 0.01. The viral titers were determined as TCID 50 .
    Figure Legend Snippet: ( a ) Strategy for the construction of a full-length DNA-launched infectious clone of PAstV. The T7 promoter of pMD123 was replaced with a cytomegalovirus (CMV) promotor using SpeI and NgoMIV . The hepatitis delta virus ribozyme was inserted into the 3′ terminal end of the PAstV-GX1 genome with XhoI and SacII. The restriction enzyme sites above the full-length cDNA clones are indicated by arrows. ( b ) Cytopathic effects were observed in PK-15 cells infected with rescued viruses (magnification 10×). ( c ) IFA analysis of the Cap protein expression in PK-15 cells. PK-15 cells infected with rescued and parental viruses were fixed at approximately 48 h.p.i and subjected to immunofluorescence using a polyclonal antibody against capsid protein. The PK-15 cells were stained with goat anti-mouse IgG H L (CoraLite488) (magnification 20×). ( d ) Growth curves of the recombinant and parental viruses. PK-15 cells were infected with recovered and parental viruses at a multiplicity of infection of 0.01. The viral titers were determined as TCID 50 .

    Techniques Used: Clone Assay, Infection, Immunofluorescence, Expressing, Staining, Recombinant

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    New England Biolabs ngomiv
    ( a ) Strategy for the construction of a full-length DNA-launched infectious clone of PAstV. The T7 promoter of pMD123 was replaced with a cytomegalovirus (CMV) promotor using <t>SpeI</t> and <t>NgoMIV</t> . The hepatitis delta virus ribozyme was inserted into the 3′ terminal end of the PAstV-GX1 genome with XhoI and SacII. The restriction enzyme sites above the full-length cDNA clones are indicated by arrows. ( b ) Cytopathic effects were observed in PK-15 cells infected with rescued viruses (magnification 10×). ( c ) IFA analysis of the Cap protein expression in PK-15 cells. PK-15 cells infected with rescued and parental viruses were fixed at approximately 48 h.p.i and subjected to immunofluorescence using a polyclonal antibody against capsid protein. The PK-15 cells were stained with goat anti-mouse IgG H L (CoraLite488) (magnification 20×). ( d ) Growth curves of the recombinant and parental viruses. PK-15 cells were infected with recovered and parental viruses at a multiplicity of infection of 0.01. The viral titers were determined as TCID 50 .
    Ngomiv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ngomiv/product/New England Biolabs
    Average 93 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    ngomiv - by Bioz Stars, 2022-09
    93/100 stars
      Buy from Supplier

    93
    New England Biolabs ngom iv
    ( a ) Strategy for the construction of a full-length DNA-launched infectious clone of PAstV. The T7 promoter of pMD123 was replaced with a cytomegalovirus (CMV) promotor using <t>SpeI</t> and <t>NgoMIV</t> . The hepatitis delta virus ribozyme was inserted into the 3′ terminal end of the PAstV-GX1 genome with XhoI and SacII. The restriction enzyme sites above the full-length cDNA clones are indicated by arrows. ( b ) Cytopathic effects were observed in PK-15 cells infected with rescued viruses (magnification 10×). ( c ) IFA analysis of the Cap protein expression in PK-15 cells. PK-15 cells infected with rescued and parental viruses were fixed at approximately 48 h.p.i and subjected to immunofluorescence using a polyclonal antibody against capsid protein. The PK-15 cells were stained with goat anti-mouse IgG H L (CoraLite488) (magnification 20×). ( d ) Growth curves of the recombinant and parental viruses. PK-15 cells were infected with recovered and parental viruses at a multiplicity of infection of 0.01. The viral titers were determined as TCID 50 .
    Ngom Iv, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ngom iv/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ngom iv - by Bioz Stars, 2022-09
    93/100 stars
      Buy from Supplier

    Image Search Results


    ( a ) Strategy for the construction of a full-length DNA-launched infectious clone of PAstV. The T7 promoter of pMD123 was replaced with a cytomegalovirus (CMV) promotor using SpeI and NgoMIV . The hepatitis delta virus ribozyme was inserted into the 3′ terminal end of the PAstV-GX1 genome with XhoI and SacII. The restriction enzyme sites above the full-length cDNA clones are indicated by arrows. ( b ) Cytopathic effects were observed in PK-15 cells infected with rescued viruses (magnification 10×). ( c ) IFA analysis of the Cap protein expression in PK-15 cells. PK-15 cells infected with rescued and parental viruses were fixed at approximately 48 h.p.i and subjected to immunofluorescence using a polyclonal antibody against capsid protein. The PK-15 cells were stained with goat anti-mouse IgG H L (CoraLite488) (magnification 20×). ( d ) Growth curves of the recombinant and parental viruses. PK-15 cells were infected with recovered and parental viruses at a multiplicity of infection of 0.01. The viral titers were determined as TCID 50 .

    Journal: Viruses

    Article Title: Insertion of Exogenous Genes within the ORF1a Coding Region of Porcine Astrovirus

    doi: 10.3390/v13112119

    Figure Lengend Snippet: ( a ) Strategy for the construction of a full-length DNA-launched infectious clone of PAstV. The T7 promoter of pMD123 was replaced with a cytomegalovirus (CMV) promotor using SpeI and NgoMIV . The hepatitis delta virus ribozyme was inserted into the 3′ terminal end of the PAstV-GX1 genome with XhoI and SacII. The restriction enzyme sites above the full-length cDNA clones are indicated by arrows. ( b ) Cytopathic effects were observed in PK-15 cells infected with rescued viruses (magnification 10×). ( c ) IFA analysis of the Cap protein expression in PK-15 cells. PK-15 cells infected with rescued and parental viruses were fixed at approximately 48 h.p.i and subjected to immunofluorescence using a polyclonal antibody against capsid protein. The PK-15 cells were stained with goat anti-mouse IgG H L (CoraLite488) (magnification 20×). ( d ) Growth curves of the recombinant and parental viruses. PK-15 cells were infected with recovered and parental viruses at a multiplicity of infection of 0.01. The viral titers were determined as TCID 50 .

    Article Snippet: The fusional fragment (CMVNgO) was digested with SpeI and NgoMIV (NEB, Beverly, USA) and ligated into pMDF123 digested with the same restriction enzymes, creating a plasmid, pMCMV.

    Techniques: Clone Assay, Infection, Immunofluorescence, Expressing, Staining, Recombinant

    Lysates of N. gonorrhoeae fragments pECFP-N1 and damage DNA from VK2/E6E7 cells. A. DNA agarose gel showing the digestion of pECFP-N1 plasmid by HindIII (positive control, lane 2), MS11 P+ lysate (lane 3), and MS11 P+ HI lysate (lane 5). Lane 5 shows bacterial MS11 P+ lysate without pECFP-N1 and lane 1 shows uncut circular pECFP-N1. B. PFGE analysis of purified VK2/E6E7 genomic DNA treated for 24 h with: lane 1: PBS (negative control), lane 2: MS11 P+ lysate, lane 3: MS11 P+ HI lysate. Lane 4 shows bacterial MS11 P+ lysate without VK2/E6E7 genomic DNA. C. Graph showing quantification of DNA smears (measured directly underneath and below the band). Shown are smear pixel intensities of cellular DNA alone and cellular DNA exposed to bacterial lysates and HI bacterial lysates. D. PFGE showing genomic DNA subjected to commercial restriction enzymes for 24 h. Lane 1: DNA incubated with CutSmart reaction buffer (negative control). Lane 2: DNA incubated with NgoMIV. Lane 3: DNA incubated with MfeI, Lane 4: DNA incubated with NgoMIV and MfeI Lane 5: DNA incubated with NgoMIV and BamHI/KpnI/MfeI (BKM).

    Journal: PLoS ONE

    Article Title: Restriction Endonucleases from Invasive Neisseria gonorrhoeae Cause Double-Strand Breaks and Distort Mitosis in Epithelial Cells during Infection

    doi: 10.1371/journal.pone.0114208

    Figure Lengend Snippet: Lysates of N. gonorrhoeae fragments pECFP-N1 and damage DNA from VK2/E6E7 cells. A. DNA agarose gel showing the digestion of pECFP-N1 plasmid by HindIII (positive control, lane 2), MS11 P+ lysate (lane 3), and MS11 P+ HI lysate (lane 5). Lane 5 shows bacterial MS11 P+ lysate without pECFP-N1 and lane 1 shows uncut circular pECFP-N1. B. PFGE analysis of purified VK2/E6E7 genomic DNA treated for 24 h with: lane 1: PBS (negative control), lane 2: MS11 P+ lysate, lane 3: MS11 P+ HI lysate. Lane 4 shows bacterial MS11 P+ lysate without VK2/E6E7 genomic DNA. C. Graph showing quantification of DNA smears (measured directly underneath and below the band). Shown are smear pixel intensities of cellular DNA alone and cellular DNA exposed to bacterial lysates and HI bacterial lysates. D. PFGE showing genomic DNA subjected to commercial restriction enzymes for 24 h. Lane 1: DNA incubated with CutSmart reaction buffer (negative control). Lane 2: DNA incubated with NgoMIV. Lane 3: DNA incubated with MfeI, Lane 4: DNA incubated with NgoMIV and MfeI Lane 5: DNA incubated with NgoMIV and BamHI/KpnI/MfeI (BKM).

    Article Snippet: As positive controls, 0.5 µl each of the restriction enzymes NgoMIV, BamHI, KpnI and MfeI (New England Biolabs, Ipswich, MA, USA) and 5 µl CutSmart buffer were added to 1 µg genomic DNA in 50 µl reactions.

    Techniques: Agarose Gel Electrophoresis, Plasmid Preparation, Positive Control, Purification, Negative Control, Incubation