tfii  (New England Biolabs)


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  • 94
    Name:
    TfiI
    Description:
    TfiI 500 units
    Catalog Number:
    R0546L
    Price:
    68
    Category:
    Restriction Enzymes
    Size:
    500 units
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    Structured Review

    New England Biolabs tfii
    TfiI
    TfiI 500 units
    https://www.bioz.com/result/tfii/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tfii - by Bioz Stars, 2021-06
    94/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Regulatory rare variants of the dopaminergic gene ANKK1 as potential risk factors for Parkinson’s disease
    Article Snippet: Sanger sequencing was performed on positive amplicons. .. Genotyping of rs7107223 Touch-down (TD)-PCR was performed using the primers rs7107223-F and rs7107223-R (Table ) and PCR products digested with TfiI (New England Biolabs, Massachusetts, USA). .. After, genotypes were visualized in 1.8% agarose gel in Tris–acetate-EDTA (TAE) 1X containing GelRed (1:10,000, Biotium).

    Article Title: Effect of RECK Gene Polymorphisms on Hepatocellular Carcinoma Susceptibility and Clinicopathologic Features
    Article Snippet: The PCR cycling conditions were 5 min at 94°C followed by 35 cycles of 1 min at 94°C, 1 min at 60°C, and 2 min at 72°C, with a final step at 72°C for 20 min. .. Ten µl aliquot of PCR product was subjected to digestion at 37°C for 4 h in a 15 µL reaction buffer containing 1.5 µL 10× buffer (New England Biolabs) and 5 U of Tfi I, Rsa I and HpyCH4 IV (New England Biolabs, Beverly, MA) for RECK rs16932912, rs11788747 and rs10972727, respectively. .. To validate results from PCR-RFLP, around 10% of assays were repeated and several cases of each genotype were confirmed by the DNA sequence analysis.

    Article Title: Developmental Xist induction is mediated by enhanced splicing
    Article Snippet: .. PCR products were digested with 2.5 units of Tfi1 (New England Biolabs cat. no. R0546L) for 2 h at 65°C. .. For single cell studies, PCR products were digested with 2.0 units of Tfi at 37°C overnight.

    Article Title: Expression Strategy of Aedes albopictus Densovirus
    Article Snippet: A canonical initiator InrNS2 sequence (CAGT) is located at nt 342. .. After TfiI digestion (NEB) at nt 338 of the A831R/IP PCR product, followed by a nested PCR (A626R/IP or A497R/IP) for specific amplification of putative NS2 transcripts, bands of 330 and 200 nt were produced (data not shown). .. Their sequencing revealed that NS2 transcription started at nt 343 (sequence data not shown).

    Article Title: Use of the Agilent 2100 Bioanalyzer for Rapid and Reproducible Molecular Typing of Streptococcus pneumoniae ▿
    Article Snippet: The PCR products (approximately 1.2 kb) were quantified using the DNA 7500 kit for the Agilent 2100 bioanalyzer (Agilent Technologies, Palo Alto, CA). .. Four hundred nanograms of each PCR product was digested separately with the restriction endonucleases DdeI, MseI, AluI, AfiIII, HaeIII, ApoI, TspRI, and TfiI (New England Biolabs, Ipswich, MA), according the manufacturer's instructions, in a total reaction mixture volume of 20 μl. .. Following digestion with DdeI, MseI, or AluI, the samples were heated to 65°C for 20 min, following digestion with the other enzymes to 80°C for 20 min, and then cooled on ice for 10 min to disrupt aggregates of DNA ( ) before 1 μl of the digestion product was analyzed using the Agilent 2100 bioanalyzer according to the manufacturer's protocol.

    Plasmid Preparation:

    Article Title: Sis1 potentiates the stress response to protein aggregation and elevated temperature
    Article Snippet: DNA fragments encoding V5 tagged DnaJB1 or DnaJB6b were amplified from pcDNA3 V5 DnaJB1 or pcDNA3 V5 DnaJB6 , using primers 5 V5 SpeI and 3 DnajB1 XhoI or 5 V5 SpeI and 3 DnajB6b XhoI, respectively, and cloned into a SpeI and XhoI (New England Biolabs) digested pRS414PGPD vector . .. The mid-length polyQ plasmid pYES2-Myc-48QmCh was created by first isolating the 48Q Htt fragment from pBacMam2-DiEx-LIC-C-flag_huntingtin_full-lengthQ48 via restriction digest, first with PvuI and XhoI, then with StuI and TfiI (New England Biolabs). .. The restriction product was integrated via Gibson Assembly into the vector created by PCR using pYES2-Myc-97QmCh as a template with primers PolyQBB_fw and int.PolyQrev.

    Nested PCR:

    Article Title: Expression Strategy of Aedes albopictus Densovirus
    Article Snippet: A canonical initiator InrNS2 sequence (CAGT) is located at nt 342. .. After TfiI digestion (NEB) at nt 338 of the A831R/IP PCR product, followed by a nested PCR (A626R/IP or A497R/IP) for specific amplification of putative NS2 transcripts, bands of 330 and 200 nt were produced (data not shown). .. Their sequencing revealed that NS2 transcription started at nt 343 (sequence data not shown).

    Amplification:

    Article Title: Expression Strategy of Aedes albopictus Densovirus
    Article Snippet: A canonical initiator InrNS2 sequence (CAGT) is located at nt 342. .. After TfiI digestion (NEB) at nt 338 of the A831R/IP PCR product, followed by a nested PCR (A626R/IP or A497R/IP) for specific amplification of putative NS2 transcripts, bands of 330 and 200 nt were produced (data not shown). .. Their sequencing revealed that NS2 transcription started at nt 343 (sequence data not shown).

    Produced:

    Article Title: Expression Strategy of Aedes albopictus Densovirus
    Article Snippet: A canonical initiator InrNS2 sequence (CAGT) is located at nt 342. .. After TfiI digestion (NEB) at nt 338 of the A831R/IP PCR product, followed by a nested PCR (A626R/IP or A497R/IP) for specific amplification of putative NS2 transcripts, bands of 330 and 200 nt were produced (data not shown). .. Their sequencing revealed that NS2 transcription started at nt 343 (sequence data not shown).

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  • 94
    New England Biolabs tfi i
    Primer design for RT-LAMP to detect IBDV based on the VP5 gene of Gx strain . (A). Genomic DNA sequence of <t>vvIBDV</t> Gx strain (GenBank Accession number: [ AY444873 ]) VP5 gene from 181 to 417 nt in orientation 5' to 3'. Nucleotide sequences used for the primers are highlighted with grey background, and the arrows indicate the 5' to 3' direction of the primers. The open box shows the <t>Tfi</t> I restriction site exclusively located in non-vvIBDVs. (B). A table listing the names and sequences of all 6 primers.
    Tfi I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tfi i/product/New England Biolabs
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    tfi i - by Bioz Stars, 2021-06
    94/100 stars
      Buy from Supplier

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    Primer design for RT-LAMP to detect IBDV based on the VP5 gene of Gx strain . (A). Genomic DNA sequence of vvIBDV Gx strain (GenBank Accession number: [ AY444873 ]) VP5 gene from 181 to 417 nt in orientation 5' to 3'. Nucleotide sequences used for the primers are highlighted with grey background, and the arrows indicate the 5' to 3' direction of the primers. The open box shows the Tfi I restriction site exclusively located in non-vvIBDVs. (B). A table listing the names and sequences of all 6 primers.

    Journal: Virology Journal

    Article Title: A one-step reverse transcription loop-mediated isothermal amplification for detection and discrimination of infectious bursal disease virus

    doi: 10.1186/1743-422X-8-108

    Figure Lengend Snippet: Primer design for RT-LAMP to detect IBDV based on the VP5 gene of Gx strain . (A). Genomic DNA sequence of vvIBDV Gx strain (GenBank Accession number: [ AY444873 ]) VP5 gene from 181 to 417 nt in orientation 5' to 3'. Nucleotide sequences used for the primers are highlighted with grey background, and the arrows indicate the 5' to 3' direction of the primers. The open box shows the Tfi I restriction site exclusively located in non-vvIBDVs. (B). A table listing the names and sequences of all 6 primers.

    Article Snippet: Identification of vvIBDV by Tfi I restriction fragment analysis RT-LAMP products were digested with Tfi I in a 20 μL reaction containing 3 μL RT-LAMP product, 1 × NEBuffer 3, 1 × BSA and 2.5 U TfiI (New England Biolabs, USA).

    Techniques: Sequencing

    Different Tfi I restriction patterns of RT-LAMP products of vvIBDV Gx and attenuated Gt strains . RT-LAMP products were digested by Tfi I and subjected to a 2% agarose gel. After ethidium bromide staining, DNA band patterns were photographed under a UV transilluminator. Lanes M, DNA marker DL2000 (TaKaRa, China, with 2000, 1000, 750, 500, 250 and 100 bp bands); 1, RT-LAMP product of Gx digested by Tfi I; 2, RT-LAMP product of Gt digested by Tfi I.

    Journal: Virology Journal

    Article Title: A one-step reverse transcription loop-mediated isothermal amplification for detection and discrimination of infectious bursal disease virus

    doi: 10.1186/1743-422X-8-108

    Figure Lengend Snippet: Different Tfi I restriction patterns of RT-LAMP products of vvIBDV Gx and attenuated Gt strains . RT-LAMP products were digested by Tfi I and subjected to a 2% agarose gel. After ethidium bromide staining, DNA band patterns were photographed under a UV transilluminator. Lanes M, DNA marker DL2000 (TaKaRa, China, with 2000, 1000, 750, 500, 250 and 100 bp bands); 1, RT-LAMP product of Gx digested by Tfi I; 2, RT-LAMP product of Gt digested by Tfi I.

    Article Snippet: Identification of vvIBDV by Tfi I restriction fragment analysis RT-LAMP products were digested with Tfi I in a 20 μL reaction containing 3 μL RT-LAMP product, 1 × NEBuffer 3, 1 × BSA and 2.5 U TfiI (New England Biolabs, USA).

    Techniques: Agarose Gel Electrophoresis, Staining, Marker