tfi i  (New England Biolabs)


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  • 94
    Name:
    TfiI
    Description:
    TfiI 500 units
    Catalog Number:
    r0546l
    Price:
    68
    Size:
    500 units
    Category:
    Restriction Enzymes
    Buy from Supplier


    Structured Review

    New England Biolabs tfi i
    TfiI
    TfiI 500 units
    https://www.bioz.com/result/tfi i/product/New England Biolabs
    Average 94 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    tfi i - by Bioz Stars, 2020-09
    94/100 stars

    Images

    1) Product Images from "A one-step reverse transcription loop-mediated isothermal amplification for detection and discrimination of infectious bursal disease virus"

    Article Title: A one-step reverse transcription loop-mediated isothermal amplification for detection and discrimination of infectious bursal disease virus

    Journal: Virology Journal

    doi: 10.1186/1743-422X-8-108

    Primer design for RT-LAMP to detect IBDV based on the VP5 gene of Gx strain . (A). Genomic DNA sequence of vvIBDV Gx strain (GenBank Accession number: [ AY444873 ]) VP5 gene from 181 to 417 nt in orientation 5' to 3'. Nucleotide sequences used for the primers are highlighted with grey background, and the arrows indicate the 5' to 3' direction of the primers. The open box shows the Tfi I restriction site exclusively located in non-vvIBDVs. (B). A table listing the names and sequences of all 6 primers.
    Figure Legend Snippet: Primer design for RT-LAMP to detect IBDV based on the VP5 gene of Gx strain . (A). Genomic DNA sequence of vvIBDV Gx strain (GenBank Accession number: [ AY444873 ]) VP5 gene from 181 to 417 nt in orientation 5' to 3'. Nucleotide sequences used for the primers are highlighted with grey background, and the arrows indicate the 5' to 3' direction of the primers. The open box shows the Tfi I restriction site exclusively located in non-vvIBDVs. (B). A table listing the names and sequences of all 6 primers.

    Techniques Used: Sequencing

    Different Tfi I restriction patterns of RT-LAMP products of vvIBDV Gx and attenuated Gt strains . RT-LAMP products were digested by Tfi I and subjected to a 2% agarose gel. After ethidium bromide staining, DNA band patterns were photographed under a UV transilluminator. Lanes M, DNA marker DL2000 (TaKaRa, China, with 2000, 1000, 750, 500, 250 and 100 bp bands); 1, RT-LAMP product of Gx digested by Tfi I; 2, RT-LAMP product of Gt digested by Tfi I.
    Figure Legend Snippet: Different Tfi I restriction patterns of RT-LAMP products of vvIBDV Gx and attenuated Gt strains . RT-LAMP products were digested by Tfi I and subjected to a 2% agarose gel. After ethidium bromide staining, DNA band patterns were photographed under a UV transilluminator. Lanes M, DNA marker DL2000 (TaKaRa, China, with 2000, 1000, 750, 500, 250 and 100 bp bands); 1, RT-LAMP product of Gx digested by Tfi I; 2, RT-LAMP product of Gt digested by Tfi I.

    Techniques Used: Agarose Gel Electrophoresis, Staining, Marker

    Related Articles

    Amplification:

    Article Title: The effect of genetic variations in the choline acetyltransferase gene (ChAT) on waterpipe tobacco smoking dependence
    Article Snippet: .. On the other hand, the 159 bp amplification product for rs1917810 was digested at 37 °C overnight by TfiI restriction enzyme (New England Biolabs, Frankfurt, Germany). ..

    Purification:

    Article Title: A trial of somatic gene targeting in vivo with an adenovirus vector
    Article Snippet: .. The 288-bp product was purified by ethanol/sodium precipitation, digested with TfiI (New England Biolabs, Beverly, MA, USA) (recognition site, 5'-G|AWTC-3' (W = A or T)) at 65°C and analyzed using agarose electrophoresis. .. The mutant sequence was resistant to TfiI, while the wild-type sequence was sensitive, yielding 204 and 84 bp fragments.

    Electrophoresis:

    Article Title: A trial of somatic gene targeting in vivo with an adenovirus vector
    Article Snippet: .. The 288-bp product was purified by ethanol/sodium precipitation, digested with TfiI (New England Biolabs, Beverly, MA, USA) (recognition site, 5'-G|AWTC-3' (W = A or T)) at 65°C and analyzed using agarose electrophoresis. .. The mutant sequence was resistant to TfiI, while the wild-type sequence was sensitive, yielding 204 and 84 bp fragments.

    Polymerase Chain Reaction:

    Article Title: Developmental Xist induction is mediated by enhanced splicing
    Article Snippet: .. PCR products were digested with 2.5 units of Tfi1 (New England Biolabs cat. no. R0546L) for 2 h at 65°C. .. For single cell studies, PCR products were digested with 2.0 units of Tfi at 37°C overnight.

    Article Title: Effect of RECK Gene Polymorphisms on Hepatocellular Carcinoma Susceptibility and Clinicopathologic Features
    Article Snippet: .. Ten µl aliquot of PCR product was subjected to digestion at 37°C for 4 h in a 15 µL reaction buffer containing 1.5 µL 10× buffer (New England Biolabs) and 5 U of Tfi I, Rsa I and HpyCH4 IV (New England Biolabs, Beverly, MA) for RECK rs16932912, rs11788747 and rs10972727, respectively. .. To validate results from PCR-RFLP, around 10% of assays were repeated and several cases of each genotype were confirmed by the DNA sequence analysis.

    Sequencing:

    Article Title: Detection of deleterious on-target effects after HDR-mediated CRISPR editing
    Article Snippet: .. Single-cell clone colonies were picked and analyzed by RFLP assay, using NEB enzymes TfiI for APPSwe , DdeI for APPIbe , XmnI for HDAC9, and Sanger sequencing as previously described . .. Cortical neuron differentiation was performed using a dual-SMAD inhibition-based protocol as described .

    RFLP Assay:

    Article Title: Detection of deleterious on-target effects after HDR-mediated CRISPR editing
    Article Snippet: .. Single-cell clone colonies were picked and analyzed by RFLP assay, using NEB enzymes TfiI for APPSwe , DdeI for APPIbe , XmnI for HDAC9, and Sanger sequencing as previously described . .. Cortical neuron differentiation was performed using a dual-SMAD inhibition-based protocol as described .

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  • 94
    New England Biolabs tfi i
    Primer design for RT-LAMP to detect IBDV based on the VP5 gene of Gx strain . (A). Genomic DNA sequence of <t>vvIBDV</t> Gx strain (GenBank Accession number: [ AY444873 ]) VP5 gene from 181 to 417 nt in orientation 5' to 3'. Nucleotide sequences used for the primers are highlighted with grey background, and the arrows indicate the 5' to 3' direction of the primers. The open box shows the <t>Tfi</t> I restriction site exclusively located in non-vvIBDVs. (B). A table listing the names and sequences of all 6 primers.
    Tfi I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tfi i/product/New England Biolabs
    Average 94 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    tfi i - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    Primer design for RT-LAMP to detect IBDV based on the VP5 gene of Gx strain . (A). Genomic DNA sequence of vvIBDV Gx strain (GenBank Accession number: [ AY444873 ]) VP5 gene from 181 to 417 nt in orientation 5' to 3'. Nucleotide sequences used for the primers are highlighted with grey background, and the arrows indicate the 5' to 3' direction of the primers. The open box shows the Tfi I restriction site exclusively located in non-vvIBDVs. (B). A table listing the names and sequences of all 6 primers.

    Journal: Virology Journal

    Article Title: A one-step reverse transcription loop-mediated isothermal amplification for detection and discrimination of infectious bursal disease virus

    doi: 10.1186/1743-422X-8-108

    Figure Lengend Snippet: Primer design for RT-LAMP to detect IBDV based on the VP5 gene of Gx strain . (A). Genomic DNA sequence of vvIBDV Gx strain (GenBank Accession number: [ AY444873 ]) VP5 gene from 181 to 417 nt in orientation 5' to 3'. Nucleotide sequences used for the primers are highlighted with grey background, and the arrows indicate the 5' to 3' direction of the primers. The open box shows the Tfi I restriction site exclusively located in non-vvIBDVs. (B). A table listing the names and sequences of all 6 primers.

    Article Snippet: Identification of vvIBDV by Tfi I restriction fragment analysis RT-LAMP products were digested with Tfi I in a 20 μL reaction containing 3 μL RT-LAMP product, 1 × NEBuffer 3, 1 × BSA and 2.5 U TfiI (New England Biolabs, USA).

    Techniques: Sequencing

    Different Tfi I restriction patterns of RT-LAMP products of vvIBDV Gx and attenuated Gt strains . RT-LAMP products were digested by Tfi I and subjected to a 2% agarose gel. After ethidium bromide staining, DNA band patterns were photographed under a UV transilluminator. Lanes M, DNA marker DL2000 (TaKaRa, China, with 2000, 1000, 750, 500, 250 and 100 bp bands); 1, RT-LAMP product of Gx digested by Tfi I; 2, RT-LAMP product of Gt digested by Tfi I.

    Journal: Virology Journal

    Article Title: A one-step reverse transcription loop-mediated isothermal amplification for detection and discrimination of infectious bursal disease virus

    doi: 10.1186/1743-422X-8-108

    Figure Lengend Snippet: Different Tfi I restriction patterns of RT-LAMP products of vvIBDV Gx and attenuated Gt strains . RT-LAMP products were digested by Tfi I and subjected to a 2% agarose gel. After ethidium bromide staining, DNA band patterns were photographed under a UV transilluminator. Lanes M, DNA marker DL2000 (TaKaRa, China, with 2000, 1000, 750, 500, 250 and 100 bp bands); 1, RT-LAMP product of Gx digested by Tfi I; 2, RT-LAMP product of Gt digested by Tfi I.

    Article Snippet: Identification of vvIBDV by Tfi I restriction fragment analysis RT-LAMP products were digested with Tfi I in a 20 μL reaction containing 3 μL RT-LAMP product, 1 × NEBuffer 3, 1 × BSA and 2.5 U TfiI (New England Biolabs, USA).

    Techniques: Agarose Gel Electrophoresis, Staining, Marker