restriction enzymes bspe1  (New England Biolabs)


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    Structured Review

    New England Biolabs restriction enzymes bspe1
    Restriction Enzymes Bspe1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction enzymes bspe1/product/New England Biolabs
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    restriction enzymes bspe1 - by Bioz Stars, 2019-12
    84/100 stars

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    Amplification:

    Article Title: Global inhibition with specific activation: how p53 and MYC redistribute the transcriptome in the DNA double-strand break response
    Article Snippet: A doxycycline-inducible pTRIPZ-derived vector (GE Dharmacon, RHS4750) was used to generate a system for inducible MYC-GFP and GFP expression. .. The vector was digested with restriction enzymes BspE1 (NEB, R0540) and MluI (NEB, R0198) and the large fragment was ligated with AgeI- (NEB, R0552) and MluI-digested PCR products obtained from amplification of plasmid p-c-Myc-pd4-EGFP-N1 ( ) using the primers 5′-TCCGTCCGGAGCCACCATGCCCCTCAACGTTAG-3′ and 5′-ACTACACGCGTAAGATACATTGATATGAGT-3′ (for MYC-GFP) or 5′-GATCCACCGGTCGCCACC-3′ and 5′-ACTACACGCGTAAGATACATTGATATGAGT-3′ (for GFP). .. To reduce the size of the plasmids to aid lentiviral packaging, each plasmid was next digested with restriction enzyme SfaAI (ThermoFisher, ER2091) and digested with PscI (ThermoFisher, ER1871).

    Construct:

    Article Title: Global inhibition with specific activation: how p53 and MYC redistribute the transcriptome in the DNA double-strand break response
    Article Snippet: The vector was digested with restriction enzymes BspE1 (NEB, R0540) and MluI (NEB, R0198) and the large fragment was ligated with AgeI- (NEB, R0552) and MluI-digested PCR products obtained from amplification of plasmid p-c-Myc-pd4-EGFP-N1 ( ) using the primers 5′-TCCGTCCGGAGCCACCATGCCCCTCAACGTTAG-3′ and 5′-ACTACACGCGTAAGATACATTGATATGAGT-3′ (for MYC-GFP) or 5′-GATCCACCGGTCGCCACC-3′ and 5′-ACTACACGCGTAAGATACATTGATATGAGT-3′ (for GFP). .. The larger fragment was ligated with a PscI- and SfaAIdigested PCR product obtained from amplification of pTRIPZ using the primers 5′-TTTGACCTTGACATGCT- 3′ and 5′-ACTTATGCGATCGCGCCCCAGCTGGTTCT-3′, resulting in plasmids pTRIPZ-del89-c-Myc-d4EGFP and pTRIPZ-del89-d4EGFP for MYC-GFP and GFP expression, respectively.

    Polymerase Chain Reaction:

    Article Title: Global inhibition with specific activation: how p53 and MYC redistribute the transcriptome in the DNA double-strand break response
    Article Snippet: A doxycycline-inducible pTRIPZ-derived vector (GE Dharmacon, RHS4750) was used to generate a system for inducible MYC-GFP and GFP expression. .. The vector was digested with restriction enzymes BspE1 (NEB, R0540) and MluI (NEB, R0198) and the large fragment was ligated with AgeI- (NEB, R0552) and MluI-digested PCR products obtained from amplification of plasmid p-c-Myc-pd4-EGFP-N1 ( ) using the primers 5′-TCCGTCCGGAGCCACCATGCCCCTCAACGTTAG-3′ and 5′-ACTACACGCGTAAGATACATTGATATGAGT-3′ (for MYC-GFP) or 5′-GATCCACCGGTCGCCACC-3′ and 5′-ACTACACGCGTAAGATACATTGATATGAGT-3′ (for GFP). .. To reduce the size of the plasmids to aid lentiviral packaging, each plasmid was next digested with restriction enzyme SfaAI (ThermoFisher, ER2091) and digested with PscI (ThermoFisher, ER1871).

    Expressing:

    Article Title: Global inhibition with specific activation: how p53 and MYC redistribute the transcriptome in the DNA double-strand break response
    Article Snippet: A doxycycline-inducible pTRIPZ-derived vector (GE Dharmacon, RHS4750) was used to generate a system for inducible MYC-GFP and GFP expression. .. The vector was digested with restriction enzymes BspE1 (NEB, R0540) and MluI (NEB, R0198) and the large fragment was ligated with AgeI- (NEB, R0552) and MluI-digested PCR products obtained from amplification of plasmid p-c-Myc-pd4-EGFP-N1 ( ) using the primers 5′-TCCGTCCGGAGCCACCATGCCCCTCAACGTTAG-3′ and 5′-ACTACACGCGTAAGATACATTGATATGAGT-3′ (for MYC-GFP) or 5′-GATCCACCGGTCGCCACC-3′ and 5′-ACTACACGCGTAAGATACATTGATATGAGT-3′ (for GFP).

    Sequencing:

    Article Title: Global inhibition with specific activation: how p53 and MYC redistribute the transcriptome in the DNA double-strand break response
    Article Snippet: The vector was digested with restriction enzymes BspE1 (NEB, R0540) and MluI (NEB, R0198) and the large fragment was ligated with AgeI- (NEB, R0552) and MluI-digested PCR products obtained from amplification of plasmid p-c-Myc-pd4-EGFP-N1 ( ) using the primers 5′-TCCGTCCGGAGCCACCATGCCCCTCAACGTTAG-3′ and 5′-ACTACACGCGTAAGATACATTGATATGAGT-3′ (for MYC-GFP) or 5′-GATCCACCGGTCGCCACC-3′ and 5′-ACTACACGCGTAAGATACATTGATATGAGT-3′ (for GFP). .. A separate smaller doxycycline-inducible MYC-GFP expression plasmid was constructed for integration into hTERT-RPE1 cells.

    Plasmid Preparation:

    Article Title: Global inhibition with specific activation: how p53 and MYC redistribute the transcriptome in the DNA double-strand break response
    Article Snippet: A doxycycline-inducible pTRIPZ-derived vector (GE Dharmacon, RHS4750) was used to generate a system for inducible MYC-GFP and GFP expression. .. The vector was digested with restriction enzymes BspE1 (NEB, R0540) and MluI (NEB, R0198) and the large fragment was ligated with AgeI- (NEB, R0552) and MluI-digested PCR products obtained from amplification of plasmid p-c-Myc-pd4-EGFP-N1 ( ) using the primers 5′-TCCGTCCGGAGCCACCATGCCCCTCAACGTTAG-3′ and 5′-ACTACACGCGTAAGATACATTGATATGAGT-3′ (for MYC-GFP) or 5′-GATCCACCGGTCGCCACC-3′ and 5′-ACTACACGCGTAAGATACATTGATATGAGT-3′ (for GFP). .. To reduce the size of the plasmids to aid lentiviral packaging, each plasmid was next digested with restriction enzyme SfaAI (ThermoFisher, ER2091) and digested with PscI (ThermoFisher, ER1871).

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    New England Biolabs restriction enzymes bspe1
    Restriction Enzymes Bspe1, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 84/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/restriction enzymes bspe1/product/New England Biolabs
    Average 84 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    restriction enzymes bspe1 - by Bioz Stars, 2019-12
    84/100 stars
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