bbsi (New England Biolabs)


Name:
BbsI
Description:
BbsI 1 500 units
Catalog Number:
r0539l
Price:
282
Category:
Restriction Enzymes
Size:
1 500 units
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Structured Review

BbsI 1 500 units
https://www.bioz.com/result/bbsi/product/New England Biolabs
Average 97 stars, based on 1 article reviews
Price from $9.99 to $1999.99
Images
1) Product Images from "90-Kilodalton Heat Shock Protein, Hsp90, as a Target for Genotyping Cryptosporidium spp. Known To Infect Humans ▿ spp. Known To Infect Humans ▿ †"
Article Title: 90-Kilodalton Heat Shock Protein, Hsp90, as a Target for Genotyping Cryptosporidium spp. Known To Infect Humans ▿ spp. Known To Infect Humans ▿ †
Journal: Eukaryotic Cell
doi: 10.1128/EC.00294-08

Figure Legend Snippet: Differentiation of gastric Cryptosporidium spp. by RFLP analysis of hsp90 PCR gene products using BbsI. Lanes 1 and 11, 100-bp molecular markers; lanes 2, 3, 6, 7, and 10, C. muris ; lanes 4, 5, 8, and 9, C. andersoni .
Techniques Used: Polymerase Chain Reaction
2) Product Images from "Bacillus SEVA siblings: A Golden Gate-based toolbox to create personalized integrative vectors for Bacillus subtilis"
Article Title: Bacillus SEVA siblings: A Golden Gate-based toolbox to create personalized integrative vectors for Bacillus subtilis
Journal: Scientific Reports
doi: 10.1038/s41598-017-14329-5

Figure Legend Snippet: Architecture of the MCS-IIS C2. This DNA-sequence is located on the cargo vector between the E. coli ori and the Bacillus antibiotic marker. The recognition sites for five type IIS restriction enzymes (AarI, BtgZI, BbsI, BsaI, BsmBI), each designed to create a 5′ GCGA-overhang are encoded on the DNA stretch. Architecture of all MCS-IIS can be found in Fig. S1 .
Techniques Used: Sequencing, Plasmid Preparation, Marker
3) Product Images from "Bacillus SEVA siblings: A Golden Gate-based toolbox to create personalized integrative vectors for Bacillus subtilis"
Article Title: Bacillus SEVA siblings: A Golden Gate-based toolbox to create personalized integrative vectors for Bacillus subtilis
Journal: Scientific Reports
doi: 10.1038/s41598-017-14329-5

Figure Legend Snippet: Architecture of the MCS-IIS C2. This DNA-sequence is located on the cargo vector between the E. coli ori and the Bacillus antibiotic marker. The recognition sites for five type IIS restriction enzymes (AarI, BtgZI, BbsI, BsaI, BsmBI), each designed to create a 5′ GCGA-overhang are encoded on the DNA stretch. Architecture of all MCS-IIS can be found in Fig. S1 .
Techniques Used: Sequencing, Plasmid Preparation, Marker
4) Product Images from "Preparation of Group I Introns for Biochemical Studies and Crystallization Assays by Native Affinity Purification"
Article Title: Preparation of Group I Introns for Biochemical Studies and Crystallization Assays by Native Affinity Purification
Journal: PLoS ONE
doi: 10.1371/journal.pone.0006740
![... of the HDV mutant [3] , while the BbsI restriction site is no longer present in the ... A general strategy to synthesize and purify group I introns within the corresponding exons. (A) General scheme of synthesis by PCR from overlapping primers followed by native purification of the transcribed RNA. The variant shown contains a group I intron (red), 5′ and 3′ exons (purple), a T7 promoter (blue), and restriction sites suitable for cloning (green). The mutated HDV, the dual SRP tag, and the Tma M protein coupled to a support are shown in orange, black, and cyan, respectively. Note that the NcoI restriction site was engineered within the 5′ end of the HDV mutant [3] , while the BbsI restriction site is no longer present in the final RNA. (B) Affinity purification chart for small-scale transcription reactions developed in this study (WB: wash buffer; CB: cleavage buffer; SB: storage buffer; RB: regeneration buffer; see Materials and Methods section).](https://storage.googleapis.com/bioz_article_images/PMC2729099/pone.0006740.g001.jpg)
Figure Legend Snippet: A general strategy to synthesize and purify group I introns within the corresponding exons. (A) General scheme of synthesis by PCR from overlapping primers followed by native purification of the transcribed RNA. The variant shown contains a group I intron (red), 5′ and 3′ exons (purple), a T7 promoter (blue), and restriction sites suitable for cloning (green). The mutated HDV, the dual SRP tag, and the Tma M protein coupled to a support are shown in orange, black, and cyan, respectively. Note that the NcoI restriction site was engineered within the 5′ end of the HDV mutant [3] , while the BbsI restriction site is no longer present in the final RNA. (B) Affinity purification chart for small-scale transcription reactions developed in this study (WB: wash buffer; CB: cleavage buffer; SB: storage buffer; RB: regeneration buffer; see Materials and Methods section).
Techniques Used: Polymerase Chain Reaction, Purification, Variant Assay, Clone Assay, Mutagenesis, Affinity Purification, Western Blot
5) Product Images from "Efficient Genome Editing Using CRISPR/Cas9 Technology in Chicory"
Article Title: Efficient Genome Editing Using CRISPR/Cas9 Technology in Chicory
Journal: International Journal of Molecular Sciences
doi: 10.3390/ijms20051155
![... LacZ promoter (counter selection marker) is surrounded by BbsI (second-generation enzyme) recognition sites, which allows the ligation ... Overview of the experimental design for CiPDS disruption. ( A ) Schematic position of the two guide RNAs (red boxes) targeting the fifth exon of CiPDS . The blue boxes indicate exons; the black lines indicate introns. ( B ) Schematic view of pKanCiU6-1p-sgRNAscaffold. Between the CiU6-1p and the sgRNA scaffold (scaff), a ccdB gene driven by the LacZ promoter (counter selection marker) is surrounded by BbsI (second-generation enzyme) recognition sites, which allows the ligation of the hybridized guide adaptor shown in C . Red B-L depicts the orientation of the BbsI recognition site which achieves the cleavage on the left side. Blue B-R depicts the orientation of the BbsI recognition site which achieves cleavage on the right side. ( C ) Sequences of the complementary guide adaptors with the 19 pb guide sequence (yellow), the transcription initiator nucleotide G (bold), and the binding sites necessary to insert the hybridized guide adaptor into the pKanCiU6-1p-sgRNAscaffold (red). ( D ) Representation of the vector resulting from the insertion of the hybridized guide adaptors in the pKanCiU6-1p-sgRNAscaffold, (note that one vector is constructed for each guide). The primers used for preparation of CiU6-1p-guide-sgRNA cassettes for Golden Gate Cloning are also shown. ( E ) Representation of the final plasmid pYLCRISPR-sgRNA1-sgRNA2 resulting from the cloning of the two cassettes into the pYLCRISPR/Cas9P 35S -B [ 36 ]. RB = Right Border, LB = Left Border, NLS = Nuclear Localization Signal.](https://storage.googleapis.com/bioz_article_images/PMC6429391/ijms-20-01155-g002.jpg)
Figure Legend Snippet: Overview of the experimental design for CiPDS disruption. ( A ) Schematic position of the two guide RNAs (red boxes) targeting the fifth exon of CiPDS . The blue boxes indicate exons; the black lines indicate introns. ( B ) Schematic view of pKanCiU6-1p-sgRNAscaffold. Between the CiU6-1p and the sgRNA scaffold (scaff), a ccdB gene driven by the LacZ promoter (counter selection marker) is surrounded by BbsI (second-generation enzyme) recognition sites, which allows the ligation of the hybridized guide adaptor shown in C . Red B-L depicts the orientation of the BbsI recognition site which achieves the cleavage on the left side. Blue B-R depicts the orientation of the BbsI recognition site which achieves cleavage on the right side. ( C ) Sequences of the complementary guide adaptors with the 19 pb guide sequence (yellow), the transcription initiator nucleotide G (bold), and the binding sites necessary to insert the hybridized guide adaptor into the pKanCiU6-1p-sgRNAscaffold (red). ( D ) Representation of the vector resulting from the insertion of the hybridized guide adaptors in the pKanCiU6-1p-sgRNAscaffold, (note that one vector is constructed for each guide). The primers used for preparation of CiU6-1p-guide-sgRNA cassettes for Golden Gate Cloning are also shown. ( E ) Representation of the final plasmid pYLCRISPR-sgRNA1-sgRNA2 resulting from the cloning of the two cassettes into the pYLCRISPR/Cas9P 35S -B [ 36 ]. RB = Right Border, LB = Left Border, NLS = Nuclear Localization Signal.
Techniques Used: Selection, Marker, Ligation, Sequencing, Binding Assay, Plasmid Preparation, Construct, Clone Assay
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