biolux gaussia luciferase assay kit  (New England Biolabs)


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    Name:
    BioLux Gaussia Luciferase Assay Kit
    Description:
    BioLux Gaussia Luciferase Assay Kit 1 000 assays
    Catalog Number:
    e3300l
    Price:
    485
    Size:
    1 000 assays
    Category:
    Cellular Biology
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    New England Biolabs biolux gaussia luciferase assay kit
    BioLux Gaussia Luciferase Assay Kit
    BioLux Gaussia Luciferase Assay Kit 1 000 assays
    https://www.bioz.com/result/biolux gaussia luciferase assay kit/product/New England Biolabs
    Average 99 stars, based on 219 article reviews
    Price from $9.99 to $1999.99
    biolux gaussia luciferase assay kit - by Bioz Stars, 2020-04
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    Centrifugation:

    Article Title: Surfactant Protein A Impairs Genital HPV16 Pseudovirus Infection by Innate Immune Cell Activation in A Murine Model
    Article Snippet: .. Cells and debris were removed by centrifugation and the remaining supernatants were analyzed for secreted Gaussia luciferase expression as a measure for infection using the Gaussia Luciferase Assay Kit (New England Biolabs) with the Fluoroscan Ascent FL (Thermo Fisher Scientific). .. Where indicated, mice were killed 24 h or 72 h post infection using halothane inhalation, and FRTs (excluding uterine horns and ovaries) were excised, snap frozen in liquid nitrogen and homogenised on ice in the presence of 500 μL 1× Cell Culture Lysis Reagent (Promega) or in the presence of 500 μL TRIzol™ reagent (Life Technologies) for further protein or gene expression analyses, respectively.

    Luciferase:

    Article Title: Development of hepatoma-derived, bidirectional oval-like cells as a model to study host interactions with hepatitis C virus during differentiation
    Article Snippet: .. Culture supernatant (20 μl) collected from cells expressing plasmids carrying the GLuc reporter was used to determine GLuc activity by using a BioLux Gaussia Luciferase Assay Kit (New England Biolabs). .. miRNA and DNA transfection miRNAs and non-targeting control miRNA (miR cont) were synthesized by Bonac (Fukuoka, Japan).

    Article Title: A Novel Role of Serotonin Receptor 2B Agonist as an Anti-Melanogenesis Agent
    Article Snippet: .. Gaussia luciferase activity in culture supernatants was determined using a Gaussia Luciferase Assay Kit (New England BioLabs, Ipswich, MA, USA). .. Luciferase activity was normalized to pGLuc-basic activity and the results were expressed as fold stimulation of luciferase activity relative to the unstimulated control.

    Article Title: Trichodysplasia spinulosa-Associated Polyomavirus Uses a Displaced Binding Site on VP1 to Engage Sialylated Glycolipids
    Article Snippet: .. PsV infection was measured after 3 days by detection of secreted luciferase according to the manufacturer’s instructions (BioLux Gaussia Luciferase Assay Kit, New England Biolabs) using an opaque 96-well microplate in a GloMax Multi-Detection System Luminometer (Promega) equipped with an autoinjector. ..

    Article Title: TGF?/BMP Type I Receptors ALK1 and ALK2 Are Essential for BMP9-induced Osteogenic Signaling in Mesenchymal Stem Cells *
    Article Snippet: .. At 24 and 48 h after transfection, Gaussia luciferase activity was measured by using the Gaussia Luciferase Assay kit (New England Biolabs) according to the manufacturer's instructions. ..

    Article Title: Lack of Evidence for a Direct Interaction of Progranulin and Tumor Necrosis Factor Receptor-1 and Tumor Necrosis Factor Receptor-2 From Cellular Binding Studies
    Article Snippet: .. Gaussia princeps luciferase activity was measured with the Gaussia luciferase Assay Kit (New England Biolabs, Frankfurt, Germany) essentially as described by the supplier. .. After starting the reaction by adding substrate-buffer solution, light emission was immediately ( < 10 s) quantified (Lucy 2 or a LUmo Luminometer; both Anthos Labtec Instruments) to minimize errors due to the decay of GpL activity.

    Article Title: Surfactant Protein A Impairs Genital HPV16 Pseudovirus Infection by Innate Immune Cell Activation in A Murine Model
    Article Snippet: .. Cells and debris were removed by centrifugation and the remaining supernatants were analyzed for secreted Gaussia luciferase expression as a measure for infection using the Gaussia Luciferase Assay Kit (New England Biolabs) with the Fluoroscan Ascent FL (Thermo Fisher Scientific). .. Where indicated, mice were killed 24 h or 72 h post infection using halothane inhalation, and FRTs (excluding uterine horns and ovaries) were excised, snap frozen in liquid nitrogen and homogenised on ice in the presence of 500 μL 1× Cell Culture Lysis Reagent (Promega) or in the presence of 500 μL TRIzol™ reagent (Life Technologies) for further protein or gene expression analyses, respectively.

    Article Title: Neutrophilic Granule Protein Is a Novel Murine LPS Antagonist
    Article Snippet: .. The culture supernatant was projected to perform luciferase assay with Gaussia Luciferase assay kit and Cypridina Luciferase assay kit (NEB) following their description. ..

    Article Title: Differential Methylation of the HPV 16 Upstream Regulatory Region during Epithelial Differentiation and Neoplastic Transformation
    Article Snippet: .. Secreted Gaussia luciferase activity was determined using the Gaussia Luciferase Assay Kit (New England Biolabs), according to the manufacturer's instructions. ..

    Article Title: Monensin inhibits cell proliferation and tumor growth of chemo-resistant pancreatic cancer cells by targeting the EGFR signaling pathway
    Article Snippet: .. At 24 h and 48 h post treatment, 50 µl of culture medium were taken for Gaussia luciferase assay using BioLux Gaussia Luciferase Assay Kit (New England Biolabs, NEB, Ipswich, MA). .. Total RNA purification and Touchdown-quantitative real-time PCR (TqPCR) Subconfluent pancreatic cancer cells were treated with different concentrations of monensin for 48 h. RNA was isolated by using TRIZOL Reagents (Invitrogen) for reverse transcription with hexamer and M-MuLV reverse transcriptase (NEB).

    Article Title: Activation of RXR and RAR signaling promotes myogenic differentiation of myoblastic C2C12 cells
    Article Snippet: .. Thus, at the indicated time points medium from the treated cells was collected for Gaussia luciferase assays using the Gaussia Luciferase Assay Kit (New England Biolabs). ..

    Article Title: GBF1, a Guanine Nucleotide Exchange Factor for Arf, Is Crucial for Coxsackievirus B3 RNA Replication ▿
    Article Snippet: .. The next day, the cells were washed and incubated with medium containing 1 μg/ml BFA for 5 h. A portion of the medium was tested for secreted luciferase activity with the Gaussia Luciferase assay kit (New England Biolabs). .. HeLa cells were plated at 20,000 per well in a 96-well plate and transfected with pGEM, pEYFP-GBF1 M832L, or pEYFP-GBF1 with Lipofectamine LTX.

    Article Title: Increased Virulence of Bloodstream Over Peripheral Isolates of P. aeruginosa Identified Through Post-transcriptional Regulation of Virulence Factors
    Article Snippet: .. Luciferase activity of cells, which corresponds to levels of NF-kB activation, collected at 0, 2, 4, and 8 h, was measured by luminescence using Biolux® Gaussia luciferase Assay kit (New England BioLabs Inc.). .. Values obtained by luminescence were normalized to the protein absorbance of the cell lysate as measured by BioRad® DC protein assay.

    Reporter Assay:

    Article Title: Neutrophilic Granule Protein Is a Novel Murine LPS Antagonist
    Article Snippet: Paragraph title: Reporter assay ... The culture supernatant was projected to perform luciferase assay with Gaussia Luciferase assay kit and Cypridina Luciferase assay kit (NEB) following their description.

    Article Title: Activation of RXR and RAR signaling promotes myogenic differentiation of myoblastic C2C12 cells
    Article Snippet: Paragraph title: 2.5. Transfection and Gaussia luciferase reporter assay ... Thus, at the indicated time points medium from the treated cells was collected for Gaussia luciferase assays using the Gaussia Luciferase Assay Kit (New England Biolabs).

    Stable Transfection:

    Article Title: Increased Virulence of Bloodstream Over Peripheral Isolates of P. aeruginosa Identified Through Post-transcriptional Regulation of Virulence Factors
    Article Snippet: THP1-Lucia™ cells which stably express an NF-kB luciferase reporter were treated with the cell-free supernatant of P. aeruginosa paired bloodstream and peripheral isolates. .. Luciferase activity of cells, which corresponds to levels of NF-kB activation, collected at 0, 2, 4, and 8 h, was measured by luminescence using Biolux® Gaussia luciferase Assay kit (New England BioLabs Inc.).

    Synthesized:

    Article Title: Development of hepatoma-derived, bidirectional oval-like cells as a model to study host interactions with hepatitis C virus during differentiation
    Article Snippet: Synthesized RNA was treated with DNase I, followed by acid phenol extraction to remove any remaining template DNA. .. Culture supernatant (20 μl) collected from cells expressing plasmids carrying the GLuc reporter was used to determine GLuc activity by using a BioLux Gaussia Luciferase Assay Kit (New England Biolabs).

    Construct:

    Article Title: Neutrophilic Granule Protein Is a Novel Murine LPS Antagonist
    Article Snippet: These constructs were co-transfected to 293 cells by FugeneHD (Promega). .. The culture supernatant was projected to perform luciferase assay with Gaussia Luciferase assay kit and Cypridina Luciferase assay kit (NEB) following their description.

    Incubation:

    Article Title: Lack of Evidence for a Direct Interaction of Progranulin and Tumor Necrosis Factor Receptor-1 and Tumor Necrosis Factor Receptor-2 From Cellular Binding Studies
    Article Snippet: Cells were then supplemented with the GpL fusion protein of interest and after an additional incubation period of 1 h, unbound proteins were removed (five washes with PBS). .. Gaussia princeps luciferase activity was measured with the Gaussia luciferase Assay Kit (New England Biolabs, Frankfurt, Germany) essentially as described by the supplier.

    Article Title: Surfactant Protein A Impairs Genital HPV16 Pseudovirus Infection by Innate Immune Cell Activation in A Murine Model
    Article Snippet: Where indicated, viral particles were incubated with purified human SP-A protein or BSA at a w/w ratio of 1:10 for 1 h at room temperature before addition of CMC and intravaginal administration. .. Cells and debris were removed by centrifugation and the remaining supernatants were analyzed for secreted Gaussia luciferase expression as a measure for infection using the Gaussia Luciferase Assay Kit (New England Biolabs) with the Fluoroscan Ascent FL (Thermo Fisher Scientific).

    Article Title: GBF1, a Guanine Nucleotide Exchange Factor for Arf, Is Crucial for Coxsackievirus B3 RNA Replication ▿
    Article Snippet: .. The next day, the cells were washed and incubated with medium containing 1 μg/ml BFA for 5 h. A portion of the medium was tested for secreted luciferase activity with the Gaussia Luciferase assay kit (New England Biolabs). .. HeLa cells were plated at 20,000 per well in a 96-well plate and transfected with pGEM, pEYFP-GBF1 M832L, or pEYFP-GBF1 with Lipofectamine LTX.

    Activity Assay:

    Article Title: Development of hepatoma-derived, bidirectional oval-like cells as a model to study host interactions with hepatitis C virus during differentiation
    Article Snippet: .. Culture supernatant (20 μl) collected from cells expressing plasmids carrying the GLuc reporter was used to determine GLuc activity by using a BioLux Gaussia Luciferase Assay Kit (New England Biolabs). .. miRNA and DNA transfection miRNAs and non-targeting control miRNA (miR cont) were synthesized by Bonac (Fukuoka, Japan).

    Article Title: A Novel Role of Serotonin Receptor 2B Agonist as an Anti-Melanogenesis Agent
    Article Snippet: .. Gaussia luciferase activity in culture supernatants was determined using a Gaussia Luciferase Assay Kit (New England BioLabs, Ipswich, MA, USA). .. Luciferase activity was normalized to pGLuc-basic activity and the results were expressed as fold stimulation of luciferase activity relative to the unstimulated control.

    Article Title: TGF?/BMP Type I Receptors ALK1 and ALK2 Are Essential for BMP9-induced Osteogenic Signaling in Mesenchymal Stem Cells *
    Article Snippet: .. At 24 and 48 h after transfection, Gaussia luciferase activity was measured by using the Gaussia Luciferase Assay kit (New England Biolabs) according to the manufacturer's instructions. ..

    Article Title: Lack of Evidence for a Direct Interaction of Progranulin and Tumor Necrosis Factor Receptor-1 and Tumor Necrosis Factor Receptor-2 From Cellular Binding Studies
    Article Snippet: .. Gaussia princeps luciferase activity was measured with the Gaussia luciferase Assay Kit (New England Biolabs, Frankfurt, Germany) essentially as described by the supplier. .. After starting the reaction by adding substrate-buffer solution, light emission was immediately ( < 10 s) quantified (Lucy 2 or a LUmo Luminometer; both Anthos Labtec Instruments) to minimize errors due to the decay of GpL activity.

    Article Title: Differential Methylation of the HPV 16 Upstream Regulatory Region during Epithelial Differentiation and Neoplastic Transformation
    Article Snippet: .. Secreted Gaussia luciferase activity was determined using the Gaussia Luciferase Assay Kit (New England Biolabs), according to the manufacturer's instructions. ..

    Article Title: GBF1, a Guanine Nucleotide Exchange Factor for Arf, Is Crucial for Coxsackievirus B3 RNA Replication ▿
    Article Snippet: .. The next day, the cells were washed and incubated with medium containing 1 μg/ml BFA for 5 h. A portion of the medium was tested for secreted luciferase activity with the Gaussia Luciferase assay kit (New England Biolabs). .. HeLa cells were plated at 20,000 per well in a 96-well plate and transfected with pGEM, pEYFP-GBF1 M832L, or pEYFP-GBF1 with Lipofectamine LTX.

    Article Title: Increased Virulence of Bloodstream Over Peripheral Isolates of P. aeruginosa Identified Through Post-transcriptional Regulation of Virulence Factors
    Article Snippet: .. Luciferase activity of cells, which corresponds to levels of NF-kB activation, collected at 0, 2, 4, and 8 h, was measured by luminescence using Biolux® Gaussia luciferase Assay kit (New England BioLabs Inc.). .. Values obtained by luminescence were normalized to the protein absorbance of the cell lysate as measured by BioRad® DC protein assay.

    Cell Culture:

    Article Title: Surfactant Protein A Impairs Genital HPV16 Pseudovirus Infection by Innate Immune Cell Activation in A Murine Model
    Article Snippet: Cells and debris were removed by centrifugation and the remaining supernatants were analyzed for secreted Gaussia luciferase expression as a measure for infection using the Gaussia Luciferase Assay Kit (New England Biolabs) with the Fluoroscan Ascent FL (Thermo Fisher Scientific). .. Where indicated, mice were killed 24 h or 72 h post infection using halothane inhalation, and FRTs (excluding uterine horns and ovaries) were excised, snap frozen in liquid nitrogen and homogenised on ice in the presence of 500 μL 1× Cell Culture Lysis Reagent (Promega) or in the presence of 500 μL TRIzol™ reagent (Life Technologies) for further protein or gene expression analyses, respectively.

    Article Title: Differential Methylation of the HPV 16 Upstream Regulatory Region during Epithelial Differentiation and Neoplastic Transformation
    Article Snippet: Paragraph title: Cell culture, transfections and luciferase assay ... Secreted Gaussia luciferase activity was determined using the Gaussia Luciferase Assay Kit (New England Biolabs), according to the manufacturer's instructions.

    Article Title: Activation of RXR and RAR signaling promotes myogenic differentiation of myoblastic C2C12 cells
    Article Snippet: Exponentially growing cells were seeded in 25cm2 cell culture flasks and transfected with 2µg per flask of pMyHC-GLuc or pTOP-Luc using LipofectAMINE (Invitrogen). .. Thus, at the indicated time points medium from the treated cells was collected for Gaussia luciferase assays using the Gaussia Luciferase Assay Kit (New England Biolabs).

    Expressing:

    Article Title: Development of hepatoma-derived, bidirectional oval-like cells as a model to study host interactions with hepatitis C virus during differentiation
    Article Snippet: .. Culture supernatant (20 μl) collected from cells expressing plasmids carrying the GLuc reporter was used to determine GLuc activity by using a BioLux Gaussia Luciferase Assay Kit (New England Biolabs). .. miRNA and DNA transfection miRNAs and non-targeting control miRNA (miR cont) were synthesized by Bonac (Fukuoka, Japan).

    Article Title: TGF?/BMP Type I Receptors ALK1 and ALK2 Are Essential for BMP9-induced Osteogenic Signaling in Mesenchymal Stem Cells *
    Article Snippet: These fragments were cloned into expression vectors pBGLuc1 and pBGLuc2, resulting in ALK1-GLuc1, ALK2-GLuc1, and BMP9-GLuc2. .. At 24 and 48 h after transfection, Gaussia luciferase activity was measured by using the Gaussia Luciferase Assay kit (New England Biolabs) according to the manufacturer's instructions.

    Article Title: Lack of Evidence for a Direct Interaction of Progranulin and Tumor Necrosis Factor Receptor-1 and Tumor Necrosis Factor Receptor-2 From Cellular Binding Studies
    Article Snippet: There was no evidence for endogenous expression of TNF in the HEK293 cells. .. Gaussia princeps luciferase activity was measured with the Gaussia luciferase Assay Kit (New England Biolabs, Frankfurt, Germany) essentially as described by the supplier.

    Article Title: Surfactant Protein A Impairs Genital HPV16 Pseudovirus Infection by Innate Immune Cell Activation in A Murine Model
    Article Snippet: .. Cells and debris were removed by centrifugation and the remaining supernatants were analyzed for secreted Gaussia luciferase expression as a measure for infection using the Gaussia Luciferase Assay Kit (New England Biolabs) with the Fluoroscan Ascent FL (Thermo Fisher Scientific). .. Where indicated, mice were killed 24 h or 72 h post infection using halothane inhalation, and FRTs (excluding uterine horns and ovaries) were excised, snap frozen in liquid nitrogen and homogenised on ice in the presence of 500 μL 1× Cell Culture Lysis Reagent (Promega) or in the presence of 500 μL TRIzol™ reagent (Life Technologies) for further protein or gene expression analyses, respectively.

    Article Title: Activation of RXR and RAR signaling promotes myogenic differentiation of myoblastic C2C12 cells
    Article Snippet: Gaussia luciferase possesses a natural secretory signal and upon expression is secreted into the cell medium. .. Thus, at the indicated time points medium from the treated cells was collected for Gaussia luciferase assays using the Gaussia Luciferase Assay Kit (New England Biolabs).

    Modification:

    Article Title: Differential Methylation of the HPV 16 Upstream Regulatory Region during Epithelial Differentiation and Neoplastic Transformation
    Article Snippet: Cell culture, transfections and luciferase assay Normal human keratinocytes (Promocell) were grown in K-SFM keratinocytes defined serum-free medium (Invitrogen). http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1462964 - R10 The human cervical cancer derived cell line C33A was grown in Dulbecco's modified Eagle medium supplemented with 10% fetal bovine serum. .. Secreted Gaussia luciferase activity was determined using the Gaussia Luciferase Assay Kit (New England Biolabs), according to the manufacturer's instructions.

    Derivative Assay:

    Article Title: Lack of Evidence for a Direct Interaction of Progranulin and Tumor Necrosis Factor Receptor-1 and Tumor Necrosis Factor Receptor-2 From Cellular Binding Studies
    Article Snippet: Binding values derived of EV-transfected cells were considered as non-specific binding and binding values obtained from the transfectants expressing the protein of interest were considered as total binding. .. Gaussia princeps luciferase activity was measured with the Gaussia luciferase Assay Kit (New England Biolabs, Frankfurt, Germany) essentially as described by the supplier.

    Article Title: Differential Methylation of the HPV 16 Upstream Regulatory Region during Epithelial Differentiation and Neoplastic Transformation
    Article Snippet: Cell culture, transfections and luciferase assay Normal human keratinocytes (Promocell) were grown in K-SFM keratinocytes defined serum-free medium (Invitrogen). http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1462964 - R10 The human cervical cancer derived cell line C33A was grown in Dulbecco's modified Eagle medium supplemented with 10% fetal bovine serum. .. Secreted Gaussia luciferase activity was determined using the Gaussia Luciferase Assay Kit (New England Biolabs), according to the manufacturer's instructions.

    Electroporation:

    Article Title: Development of hepatoma-derived, bidirectional oval-like cells as a model to study host interactions with hepatitis C virus during differentiation
    Article Snippet: Paragraph title: RNA synthesis and electroporation ... Culture supernatant (20 μl) collected from cells expressing plasmids carrying the GLuc reporter was used to determine GLuc activity by using a BioLux Gaussia Luciferase Assay Kit (New England Biolabs).

    Transfection:

    Article Title: TGF?/BMP Type I Receptors ALK1 and ALK2 Are Essential for BMP9-induced Osteogenic Signaling in Mesenchymal Stem Cells *
    Article Snippet: .. At 24 and 48 h after transfection, Gaussia luciferase activity was measured by using the Gaussia Luciferase Assay kit (New England Biolabs) according to the manufacturer's instructions. ..

    Article Title: Lack of Evidence for a Direct Interaction of Progranulin and Tumor Necrosis Factor Receptor-1 and Tumor Necrosis Factor Receptor-2 From Cellular Binding Studies
    Article Snippet: Please note, the expression levels observed after transfection of expression plasmids encoding TNFR1 and TNFR2 were regularly > 100-fold higher than those of endogenously expressed TNFR1. .. Gaussia princeps luciferase activity was measured with the Gaussia luciferase Assay Kit (New England Biolabs, Frankfurt, Germany) essentially as described by the supplier.

    Article Title: Neutrophilic Granule Protein Is a Novel Murine LPS Antagonist
    Article Snippet: Transfected cells were attached in a 96-well plate, and then 0, 1, 10, or 100 ng/ml of LPS was treated overnight. .. The culture supernatant was projected to perform luciferase assay with Gaussia Luciferase assay kit and Cypridina Luciferase assay kit (NEB) following their description.

    Article Title: Differential Methylation of the HPV 16 Upstream Regulatory Region during Epithelial Differentiation and Neoplastic Transformation
    Article Snippet: Paragraph title: Cell culture, transfections and luciferase assay ... Secreted Gaussia luciferase activity was determined using the Gaussia Luciferase Assay Kit (New England Biolabs), according to the manufacturer's instructions.

    Article Title: Monensin inhibits cell proliferation and tumor growth of chemo-resistant pancreatic cancer cells by targeting the EGFR signaling pathway
    Article Snippet: Paragraph title: Transfection and Gaussia luciferase assay ... At 24 h and 48 h post treatment, 50 µl of culture medium were taken for Gaussia luciferase assay using BioLux Gaussia Luciferase Assay Kit (New England Biolabs, NEB, Ipswich, MA).

    Article Title: Activation of RXR and RAR signaling promotes myogenic differentiation of myoblastic C2C12 cells
    Article Snippet: Paragraph title: 2.5. Transfection and Gaussia luciferase reporter assay ... Thus, at the indicated time points medium from the treated cells was collected for Gaussia luciferase assays using the Gaussia Luciferase Assay Kit (New England Biolabs).

    Article Title: GBF1, a Guanine Nucleotide Exchange Factor for Arf, Is Crucial for Coxsackievirus B3 RNA Replication ▿
    Article Snippet: HeLa cells were plated at 40,000 per well in a 96-well plate and transfected with pGEM (control), pEYFP-GBF1 M832L, or pEYFP-GBF1 A795E and pCMV-Gluc with Lipofectamine LTX (Invitrogen). .. The next day, the cells were washed and incubated with medium containing 1 μg/ml BFA for 5 h. A portion of the medium was tested for secreted luciferase activity with the Gaussia Luciferase assay kit (New England Biolabs).

    Infection:

    Article Title: Trichodysplasia spinulosa-Associated Polyomavirus Uses a Displaced Binding Site on VP1 to Engage Sialylated Glycolipids
    Article Snippet: .. PsV infection was measured after 3 days by detection of secreted luciferase according to the manufacturer’s instructions (BioLux Gaussia Luciferase Assay Kit, New England Biolabs) using an opaque 96-well microplate in a GloMax Multi-Detection System Luminometer (Promega) equipped with an autoinjector. ..

    Article Title: Surfactant Protein A Impairs Genital HPV16 Pseudovirus Infection by Innate Immune Cell Activation in A Murine Model
    Article Snippet: .. Cells and debris were removed by centrifugation and the remaining supernatants were analyzed for secreted Gaussia luciferase expression as a measure for infection using the Gaussia Luciferase Assay Kit (New England Biolabs) with the Fluoroscan Ascent FL (Thermo Fisher Scientific). .. Where indicated, mice were killed 24 h or 72 h post infection using halothane inhalation, and FRTs (excluding uterine horns and ovaries) were excised, snap frozen in liquid nitrogen and homogenised on ice in the presence of 500 μL 1× Cell Culture Lysis Reagent (Promega) or in the presence of 500 μL TRIzol™ reagent (Life Technologies) for further protein or gene expression analyses, respectively.

    Generated:

    Article Title: Trichodysplasia spinulosa-Associated Polyomavirus Uses a Displaced Binding Site on VP1 to Engage Sialylated Glycolipids
    Article Snippet: Mock PsV controls were generated by transfecting 293TT cells with control plasmid and the phGluc reporter plasmid in a 7:1 ratio and then purifying according to the PsV purification protocol. .. PsV infection was measured after 3 days by detection of secreted luciferase according to the manufacturer’s instructions (BioLux Gaussia Luciferase Assay Kit, New England Biolabs) using an opaque 96-well microplate in a GloMax Multi-Detection System Luminometer (Promega) equipped with an autoinjector.

    Protein-Fragment Complementation Assay:

    Article Title: TGF?/BMP Type I Receptors ALK1 and ALK2 Are Essential for BMP9-induced Osteogenic Signaling in Mesenchymal Stem Cells *
    Article Snippet: Paragraph title: Protein Fragment Complementation Assay (PCA) ... At 24 and 48 h after transfection, Gaussia luciferase activity was measured by using the Gaussia Luciferase Assay kit (New England Biolabs) according to the manufacturer's instructions.

    Injection:

    Article Title: Surfactant Protein A Impairs Genital HPV16 Pseudovirus Infection by Innate Immune Cell Activation in A Murine Model
    Article Snippet: 6 h prior HPV infection, mice were lightly anaesthetized by intra-peritoneal injection of 125 µL ketamine (1.5 mg/20 g) and xylazine (0.2 mg/20 g) and intravaginally received 25 µL of 4% Nonoxynol-9 (N-9) (abcam) in a formulation of 3% carboxymethylcellulose (CMC) in order to chemically disrupt the genital epithelial and to promote HPV infection [ ]. .. Cells and debris were removed by centrifugation and the remaining supernatants were analyzed for secreted Gaussia luciferase expression as a measure for infection using the Gaussia Luciferase Assay Kit (New England Biolabs) with the Fluoroscan Ascent FL (Thermo Fisher Scientific).

    Binding Assay:

    Article Title: Lack of Evidence for a Direct Interaction of Progranulin and Tumor Necrosis Factor Receptor-1 and Tumor Necrosis Factor Receptor-2 From Cellular Binding Studies
    Article Snippet: Paragraph title: Binding Studies ... Gaussia princeps luciferase activity was measured with the Gaussia luciferase Assay Kit (New England Biolabs, Frankfurt, Germany) essentially as described by the supplier.

    DC Protein Assay:

    Article Title: Increased Virulence of Bloodstream Over Peripheral Isolates of P. aeruginosa Identified Through Post-transcriptional Regulation of Virulence Factors
    Article Snippet: Luciferase activity of cells, which corresponds to levels of NF-kB activation, collected at 0, 2, 4, and 8 h, was measured by luminescence using Biolux® Gaussia luciferase Assay kit (New England BioLabs Inc.). .. Values obtained by luminescence were normalized to the protein absorbance of the cell lysate as measured by BioRad® DC protein assay.

    Mouse Assay:

    Article Title: Surfactant Protein A Impairs Genital HPV16 Pseudovirus Infection by Innate Immune Cell Activation in A Murine Model
    Article Snippet: For HPV infections, mice were again lightly anaesthetized and intravaginally inoculated with 1 µg HPV16-PsVs encapsidating the plasmid pCMV-GLuc2 or 3 µg virus encapsidating the plasmid pGL3-control in a total volume of 20 µL in the presence of 3% CMC formulation. .. Cells and debris were removed by centrifugation and the remaining supernatants were analyzed for secreted Gaussia luciferase expression as a measure for infection using the Gaussia Luciferase Assay Kit (New England Biolabs) with the Fluoroscan Ascent FL (Thermo Fisher Scientific).

    Lysis:

    Article Title: Surfactant Protein A Impairs Genital HPV16 Pseudovirus Infection by Innate Immune Cell Activation in A Murine Model
    Article Snippet: Cells and debris were removed by centrifugation and the remaining supernatants were analyzed for secreted Gaussia luciferase expression as a measure for infection using the Gaussia Luciferase Assay Kit (New England Biolabs) with the Fluoroscan Ascent FL (Thermo Fisher Scientific). .. Where indicated, mice were killed 24 h or 72 h post infection using halothane inhalation, and FRTs (excluding uterine horns and ovaries) were excised, snap frozen in liquid nitrogen and homogenised on ice in the presence of 500 μL 1× Cell Culture Lysis Reagent (Promega) or in the presence of 500 μL TRIzol™ reagent (Life Technologies) for further protein or gene expression analyses, respectively.

    Purification:

    Article Title: Trichodysplasia spinulosa-Associated Polyomavirus Uses a Displaced Binding Site on VP1 to Engage Sialylated Glycolipids
    Article Snippet: Mock PsV controls were generated by transfecting 293TT cells with control plasmid and the phGluc reporter plasmid in a 7:1 ratio and then purifying according to the PsV purification protocol. .. PsV infection was measured after 3 days by detection of secreted luciferase according to the manufacturer’s instructions (BioLux Gaussia Luciferase Assay Kit, New England Biolabs) using an opaque 96-well microplate in a GloMax Multi-Detection System Luminometer (Promega) equipped with an autoinjector.

    Article Title: Surfactant Protein A Impairs Genital HPV16 Pseudovirus Infection by Innate Immune Cell Activation in A Murine Model
    Article Snippet: Where indicated, viral particles were incubated with purified human SP-A protein or BSA at a w/w ratio of 1:10 for 1 h at room temperature before addition of CMC and intravaginal administration. .. Cells and debris were removed by centrifugation and the remaining supernatants were analyzed for secreted Gaussia luciferase expression as a measure for infection using the Gaussia Luciferase Assay Kit (New England Biolabs) with the Fluoroscan Ascent FL (Thermo Fisher Scientific).

    Plasmid Preparation:

    Article Title: Trichodysplasia spinulosa-Associated Polyomavirus Uses a Displaced Binding Site on VP1 to Engage Sialylated Glycolipids
    Article Snippet: Mock PsV controls were generated by transfecting 293TT cells with control plasmid and the phGluc reporter plasmid in a 7:1 ratio and then purifying according to the PsV purification protocol. .. PsV infection was measured after 3 days by detection of secreted luciferase according to the manufacturer’s instructions (BioLux Gaussia Luciferase Assay Kit, New England Biolabs) using an opaque 96-well microplate in a GloMax Multi-Detection System Luminometer (Promega) equipped with an autoinjector.

    Article Title: Surfactant Protein A Impairs Genital HPV16 Pseudovirus Infection by Innate Immune Cell Activation in A Murine Model
    Article Snippet: For HPV infections, mice were again lightly anaesthetized and intravaginally inoculated with 1 µg HPV16-PsVs encapsidating the plasmid pCMV-GLuc2 or 3 µg virus encapsidating the plasmid pGL3-control in a total volume of 20 µL in the presence of 3% CMC formulation. .. Cells and debris were removed by centrifugation and the remaining supernatants were analyzed for secreted Gaussia luciferase expression as a measure for infection using the Gaussia Luciferase Assay Kit (New England Biolabs) with the Fluoroscan Ascent FL (Thermo Fisher Scientific).

    Article Title: Neutrophilic Granule Protein Is a Novel Murine LPS Antagonist
    Article Snippet: Reporter assay the pGluc-NF-κB construct was obtained from Genecopoeia (Rockville, MD, USA). pSV40-CLuc control plasmid was obtained from NEB (Ipswich, MA, USA). .. The culture supernatant was projected to perform luciferase assay with Gaussia Luciferase assay kit and Cypridina Luciferase assay kit (NEB) following their description.

    Article Title: Differential Methylation of the HPV 16 Upstream Regulatory Region during Epithelial Differentiation and Neoplastic Transformation
    Article Snippet: Secreted Gaussia luciferase activity was determined using the Gaussia Luciferase Assay Kit (New England Biolabs), according to the manufacturer's instructions. .. The pSV-ß-Galactosidase Control Vector (Promega) was used as ß-galactosidase internal controls for transfection efficiency, and all luciferase activity measurements were corrected for ß-galactosidase activity.

    Software:

    Article Title: Lack of Evidence for a Direct Interaction of Progranulin and Tumor Necrosis Factor Receptor-1 and Tumor Necrosis Factor Receptor-2 From Cellular Binding Studies
    Article Snippet: Gaussia princeps luciferase activity was measured with the Gaussia luciferase Assay Kit (New England Biolabs, Frankfurt, Germany) essentially as described by the supplier. .. Data are reported as mean ± SEM and were analyzed by Bonferroni’s test or were analyzed with the “nonlinear regression to a one-site specific binding curve” or the “nonlinear regression to a one-site competitive binding curve” function of the GraphPad Prism5 software.

    Activation Assay:

    Article Title: Increased Virulence of Bloodstream Over Peripheral Isolates of P. aeruginosa Identified Through Post-transcriptional Regulation of Virulence Factors
    Article Snippet: .. Luciferase activity of cells, which corresponds to levels of NF-kB activation, collected at 0, 2, 4, and 8 h, was measured by luminescence using Biolux® Gaussia luciferase Assay kit (New England BioLabs Inc.). .. Values obtained by luminescence were normalized to the protein absorbance of the cell lysate as measured by BioRad® DC protein assay.

    Rescue Assay:

    Article Title: GBF1, a Guanine Nucleotide Exchange Factor for Arf, Is Crucial for Coxsackievirus B3 RNA Replication ▿
    Article Snippet: Paragraph title: Secretion rescue assay. ... The next day, the cells were washed and incubated with medium containing 1 μg/ml BFA for 5 h. A portion of the medium was tested for secreted luciferase activity with the Gaussia Luciferase assay kit (New England Biolabs).

    Proliferation Assay:

    Article Title: Trichodysplasia spinulosa-Associated Polyomavirus Uses a Displaced Binding Site on VP1 to Engage Sialylated Glycolipids
    Article Snippet: PsV infection was measured after 3 days by detection of secreted luciferase according to the manufacturer’s instructions (BioLux Gaussia Luciferase Assay Kit, New England Biolabs) using an opaque 96-well microplate in a GloMax Multi-Detection System Luminometer (Promega) equipped with an autoinjector. .. The Cell Titer 96 Aqueous Non-Radioactive Cell Proliferation Assay (Promega) was used according to the manufactor’s protocol to test for toxicity after cell treatments and the absorption was measured at 450 nm.

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  • 99
    New England Biolabs pmli
    Pmli, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pmli/product/New England Biolabs
    Average 99 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    pmli - by Bioz Stars, 2020-04
    99/100 stars
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