drdi  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Name:
    DrdI
    Description:
    DrdI 1 500 units
    Catalog Number:
    R0530L
    Price:
    282
    Category:
    Restriction Enzymes
    Size:
    1 500 units
    Buy from Supplier


    Structured Review

    New England Biolabs drdi
    DrdI
    DrdI 1 500 units
    https://www.bioz.com/result/drdi/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    drdi - by Bioz Stars, 2021-06
    93/100 stars

    Images

    1) Product Images from "A novel splice-site mutation of TULP1 underlies severe early-onset retinitis pigmentosa in a consanguineous Israeli Muslim Arab family"

    Article Title: A novel splice-site mutation of TULP1 underlies severe early-onset retinitis pigmentosa in a consanguineous Israeli Muslim Arab family

    Journal: Molecular Vision

    doi:

    The c.1495+2_1495+3insT mutation. A : Shown are nucleotide sequence traces of the boundary between TULP1 exon and intron14 in a non-carrier individual (wt), an individual heterozygote for the c.1495+2_1495+3insT mutation (het), and an affected individual homozygote for the mutant allele (mut). The exon-intron boundary is marked. B : A mismatch-primer restriction–based assay used to screen control DNA samples for the c.1495+2_1495+3insT mutation. The reverse PCR primer included one mismatched base, which, in combination with the mutant allele, but not with the WT allele, created a DrdI restriction site. A 286 bp PCR product derived from the WT allele was not digested by DrdI, while in the presence of the MUT two restriction fragments of 251 and 35 bp were obtained. Digestion of a PCR product derived from a heterozygote for the mutation (HET) yielded three fragments (286, 251, and 35 bp). The 35 bp fragment is not visible.
    Figure Legend Snippet: The c.1495+2_1495+3insT mutation. A : Shown are nucleotide sequence traces of the boundary between TULP1 exon and intron14 in a non-carrier individual (wt), an individual heterozygote for the c.1495+2_1495+3insT mutation (het), and an affected individual homozygote for the mutant allele (mut). The exon-intron boundary is marked. B : A mismatch-primer restriction–based assay used to screen control DNA samples for the c.1495+2_1495+3insT mutation. The reverse PCR primer included one mismatched base, which, in combination with the mutant allele, but not with the WT allele, created a DrdI restriction site. A 286 bp PCR product derived from the WT allele was not digested by DrdI, while in the presence of the MUT two restriction fragments of 251 and 35 bp were obtained. Digestion of a PCR product derived from a heterozygote for the mutation (HET) yielded three fragments (286, 251, and 35 bp). The 35 bp fragment is not visible.

    Techniques Used: Mutagenesis, Sequencing, Restriction Assay, Polymerase Chain Reaction, Derivative Assay

    Related Articles

    Amplification:

    Article Title: Structural basis for adhesion G protein-coupled receptor Gpr126 function
    Article Snippet: .. After amplification by PCR, the product was digested with either DrdI (NEB) at 37°C or BstUI (NEB) at 60°C, and then run on a 3% agarose gel. .. The mutation both disrupts a DrdI binding site and introduces a BstUI binding site.

    Article Title: Structural basis for adhesion G protein-coupled receptor Gpr126 function
    Article Snippet: .. After amplification by PCR, the product was digested with either DrdI (NEB) at 37 °C or BstUI (NEB) at 60 °C, and then run on a 3% agarose gel. .. The mutation both disrupts a DrdI binding site and introduces a BstUI binding site.

    Polymerase Chain Reaction:

    Article Title: Structural basis for adhesion G protein-coupled receptor Gpr126 function
    Article Snippet: .. After amplification by PCR, the product was digested with either DrdI (NEB) at 37°C or BstUI (NEB) at 60°C, and then run on a 3% agarose gel. .. The mutation both disrupts a DrdI binding site and introduces a BstUI binding site.

    Article Title: Polymorphisms in steroidogenesis genes, sex steroid levels, and high myopia in the Taiwanese population
    Article Snippet: The PCR reactions were performed in 25-μl aliquots containing 20 pmole of each primer, 1× reaction buffer, 100 µM deoxynucleotide triphosphates, and 1 unit of Taq polymerase; a PCR system 9700 thermocycler (Applied Biosystems, Foster City, CA) was used for cycling. .. The temperatures used during PCR were as follows: for rs743572 , 95 °C for 2 min, followed by 30 cycles of 95 °C for 1 min, 57 °C for 1 min, and 72 °C for 1 min, with a final extension at 72 °C for 10 min; for rs6203 and rs605059 , 95 °C for 2 min, followed by 30 cycles of 95 °C for 30 s, 60 °C for 30 s, and 72 °C for 30 s, with a final extension at 72 °C for 10 min; for rs1047303 , 95 °C for 2 min, followed by 40 cycles of 95 °C for 30 s, 50 °C for 30 s, and 72 °C for 30 s, with a final extension at 72 °C for 10 min; and for rs523349 and rs10046 , 95 °C for 2 min, followed by 30 cycles of 95 °C for 30 s, 52 °C for 30 s, and 72 °C for 30 s, with a final extension at 72 °C for 10 min. Aliquots of PCR products were digested with the following restriction enzymes and conditions: MspA1I for rs743572 at 37 °C for 3 h, BglI for rs6203 at 37 °C overnight, DrdI for rs1047303 at 37 °C overnight, BtsI for rs605059 at 55 °C overnight, HpyCH4V for rs523349 at 37 °C overnight, and BsrCI for rs10046 at 65 °C overnight, in reaction mixtures containing 10 μl of PCR product, digestion buffer, BSA (BSA), and 5 units of restriction enzyme (New England Biolabs, Ipswich, MA). .. The digested fragments were visualized on 4% agarose gel with ethidium bromide staining to identify the base pair change.

    Article Title: Single-Cell RT-PCR and Functional Characterization of Ca2+ Channels in Motoneurons of the Rat Facial Nucleus
    Article Snippet: The product of the first PCR was cut out of the agarose gel and used for a second amplification step. .. Five enzymes were chosen, Drd I, Bpm I, Hinc II, Afl II, and Acc I (New England Biolabs, Schwalbach, Germany), which selectively cut the brain α1A , α1B , α1C , α1D , and α1E isoform PCR fragments, respectively. .. The calculated lengths of the fragments generated by the restriction enzymes are shown in Figure .

    Article Title: Structural basis for adhesion G protein-coupled receptor Gpr126 function
    Article Snippet: .. After amplification by PCR, the product was digested with either DrdI (NEB) at 37 °C or BstUI (NEB) at 60 °C, and then run on a 3% agarose gel. .. The mutation both disrupts a DrdI binding site and introduces a BstUI binding site.

    Article Title: A novel splice-site mutation of TULP1 underlies severe early-onset retinitis pigmentosa in a consanguineous Israeli Muslim Arab family
    Article Snippet: The reverse primer includes one mismatched base (underlined), which, in combination with the mutant allele, but not with the wild-type (WT) allele, creates a DrdI restriction site (GAC NNN NNN GTC). .. Twenty μl of the PCR product were digested with 3 U DrdI and 1X of the recommended buffer (New England Biolabs, Beverly, MA) for several hours at 37 °C. .. The entire digestion volume (30 μl) was visualized by electrophoresis on a 5% NuSieve 3:1 agarose gel (Cambrex Bio Science, Rockland, ME) in 1XTBE buffer.

    Agarose Gel Electrophoresis:

    Article Title: Structural basis for adhesion G protein-coupled receptor Gpr126 function
    Article Snippet: .. After amplification by PCR, the product was digested with either DrdI (NEB) at 37°C or BstUI (NEB) at 60°C, and then run on a 3% agarose gel. .. The mutation both disrupts a DrdI binding site and introduces a BstUI binding site.

    Article Title: Structural basis for adhesion G protein-coupled receptor Gpr126 function
    Article Snippet: .. After amplification by PCR, the product was digested with either DrdI (NEB) at 37 °C or BstUI (NEB) at 60 °C, and then run on a 3% agarose gel. .. The mutation both disrupts a DrdI binding site and introduces a BstUI binding site.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    New England Biolabs drd i
    Single-cell RT-PCR analysis of Ca 2+ channel α 1 -subunit RNA expression in single neurons. Agarose gel electrophoresis of the cDNA amplified products from four single cells: ( A ) a cerebellar Purkinje neuron (Pn2), ( B ) a granule cell of the hippocampal dentate gyrus (Gc5), and ( C , D ) motoneurons from the facial nucleus (Mn24 and Mn20, respectively). Lanes marked M show the molecular weight marker φX174/ Hae III. The lanes marked A-S show the band corresponding to the fragments obtained after the first PCR. Lanes marked A, B, C, D, E, and S show the fragments obtained after a second PCR reaction and restriction digest with the enzymes <t>Drd</t> I, Bpm I, Hinc II, Afl II, Acc I and Cla I, respectively.
    Drd I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/drd i/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    drd i - by Bioz Stars, 2021-06
    93/100 stars
      Buy from Supplier

    Image Search Results


    Single-cell RT-PCR analysis of Ca 2+ channel α 1 -subunit RNA expression in single neurons. Agarose gel electrophoresis of the cDNA amplified products from four single cells: ( A ) a cerebellar Purkinje neuron (Pn2), ( B ) a granule cell of the hippocampal dentate gyrus (Gc5), and ( C , D ) motoneurons from the facial nucleus (Mn24 and Mn20, respectively). Lanes marked M show the molecular weight marker φX174/ Hae III. The lanes marked A-S show the band corresponding to the fragments obtained after the first PCR. Lanes marked A, B, C, D, E, and S show the fragments obtained after a second PCR reaction and restriction digest with the enzymes Drd I, Bpm I, Hinc II, Afl II, Acc I and Cla I, respectively.

    Journal: The Journal of Neuroscience

    Article Title: Single-Cell RT-PCR and Functional Characterization of Ca2+ Channels in Motoneurons of the Rat Facial Nucleus

    doi: 10.1523/JNEUROSCI.18-23-09573.1998

    Figure Lengend Snippet: Single-cell RT-PCR analysis of Ca 2+ channel α 1 -subunit RNA expression in single neurons. Agarose gel electrophoresis of the cDNA amplified products from four single cells: ( A ) a cerebellar Purkinje neuron (Pn2), ( B ) a granule cell of the hippocampal dentate gyrus (Gc5), and ( C , D ) motoneurons from the facial nucleus (Mn24 and Mn20, respectively). Lanes marked M show the molecular weight marker φX174/ Hae III. The lanes marked A-S show the band corresponding to the fragments obtained after the first PCR. Lanes marked A, B, C, D, E, and S show the fragments obtained after a second PCR reaction and restriction digest with the enzymes Drd I, Bpm I, Hinc II, Afl II, Acc I and Cla I, respectively.

    Article Snippet: Five enzymes were chosen, Drd I, Bpm I, Hinc II, Afl II, and Acc I (New England Biolabs, Schwalbach, Germany), which selectively cut the brain α1A , α1B , α1C , α1D , and α1E isoform PCR fragments, respectively.

    Techniques: Reverse Transcription Polymerase Chain Reaction, RNA Expression, Agarose Gel Electrophoresis, Amplification, Molecular Weight, Marker, Polymerase Chain Reaction

    RT-PCR analysis of Ca 2+ channel α 1 -subunits. A , Ethidium bromide-stained gel (1.5%) showing the amplified fragments produced when 10, 1, 0.1, and 0.01 ng of input cDNA from whole-brain total RNA were used in the PCR. The lanes marked M and W are the molecular weight marker φX174/ Hae II and the RT-PCR without RNA, respectively. B , Ethidium bromide-stained gel (1.5%) showing the absence of amplification of genomic DNA. Lane 1 , RT-PCR with 10 ng of total RNA from rat brain. Lane 2 , RT-PCR with 10 ng of total RNA from whole brain, but without reverse transcriptase. Lane 3 , PCR with 200 ng of genomic DNA. Lane 4 , RT-PCR without RNA. M , Molecular weight marker. C , Analysis of Ca 2+ channel α 1 -subunits in RNA from whole brain (adult). The lane marked A-S shows the band corresponding to the fragments obtained after the first PCR. Lanes marked A, B, C, D, E , and S show the fragments obtained after a second PCR reaction and restriction digest with the enzymes Drd I, Bpm I, Hinc II, Afl II, Acc I, and Cla I, respectively.

    Journal: The Journal of Neuroscience

    Article Title: Single-Cell RT-PCR and Functional Characterization of Ca2+ Channels in Motoneurons of the Rat Facial Nucleus

    doi: 10.1523/JNEUROSCI.18-23-09573.1998

    Figure Lengend Snippet: RT-PCR analysis of Ca 2+ channel α 1 -subunits. A , Ethidium bromide-stained gel (1.5%) showing the amplified fragments produced when 10, 1, 0.1, and 0.01 ng of input cDNA from whole-brain total RNA were used in the PCR. The lanes marked M and W are the molecular weight marker φX174/ Hae II and the RT-PCR without RNA, respectively. B , Ethidium bromide-stained gel (1.5%) showing the absence of amplification of genomic DNA. Lane 1 , RT-PCR with 10 ng of total RNA from rat brain. Lane 2 , RT-PCR with 10 ng of total RNA from whole brain, but without reverse transcriptase. Lane 3 , PCR with 200 ng of genomic DNA. Lane 4 , RT-PCR without RNA. M , Molecular weight marker. C , Analysis of Ca 2+ channel α 1 -subunits in RNA from whole brain (adult). The lane marked A-S shows the band corresponding to the fragments obtained after the first PCR. Lanes marked A, B, C, D, E , and S show the fragments obtained after a second PCR reaction and restriction digest with the enzymes Drd I, Bpm I, Hinc II, Afl II, Acc I, and Cla I, respectively.

    Article Snippet: Five enzymes were chosen, Drd I, Bpm I, Hinc II, Afl II, and Acc I (New England Biolabs, Schwalbach, Germany), which selectively cut the brain α1A , α1B , α1C , α1D , and α1E isoform PCR fragments, respectively.

    Techniques: Reverse Transcription Polymerase Chain Reaction, Staining, Amplification, Produced, Polymerase Chain Reaction, Molecular Weight, Marker

    The c.1495+2_1495+3insT mutation. A : Shown are nucleotide sequence traces of the boundary between TULP1 exon and intron14 in a non-carrier individual (wt), an individual heterozygote for the c.1495+2_1495+3insT mutation (het), and an affected individual homozygote for the mutant allele (mut). The exon-intron boundary is marked. B : A mismatch-primer restriction–based assay used to screen control DNA samples for the c.1495+2_1495+3insT mutation. The reverse PCR primer included one mismatched base, which, in combination with the mutant allele, but not with the WT allele, created a DrdI restriction site. A 286 bp PCR product derived from the WT allele was not digested by DrdI, while in the presence of the MUT two restriction fragments of 251 and 35 bp were obtained. Digestion of a PCR product derived from a heterozygote for the mutation (HET) yielded three fragments (286, 251, and 35 bp). The 35 bp fragment is not visible.

    Journal: Molecular Vision

    Article Title: A novel splice-site mutation of TULP1 underlies severe early-onset retinitis pigmentosa in a consanguineous Israeli Muslim Arab family

    doi:

    Figure Lengend Snippet: The c.1495+2_1495+3insT mutation. A : Shown are nucleotide sequence traces of the boundary between TULP1 exon and intron14 in a non-carrier individual (wt), an individual heterozygote for the c.1495+2_1495+3insT mutation (het), and an affected individual homozygote for the mutant allele (mut). The exon-intron boundary is marked. B : A mismatch-primer restriction–based assay used to screen control DNA samples for the c.1495+2_1495+3insT mutation. The reverse PCR primer included one mismatched base, which, in combination with the mutant allele, but not with the WT allele, created a DrdI restriction site. A 286 bp PCR product derived from the WT allele was not digested by DrdI, while in the presence of the MUT two restriction fragments of 251 and 35 bp were obtained. Digestion of a PCR product derived from a heterozygote for the mutation (HET) yielded three fragments (286, 251, and 35 bp). The 35 bp fragment is not visible.

    Article Snippet: Twenty μl of the PCR product were digested with 3 U DrdI and 1X of the recommended buffer (New England Biolabs, Beverly, MA) for several hours at 37 °C.

    Techniques: Mutagenesis, Sequencing, Restriction Assay, Polymerase Chain Reaction, Derivative Assay