bsmai  (New England Biolabs)


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    BsmAI 5 000 units
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    New England Biolabs bsmai
    BsmAI
    BsmAI 5 000 units
    https://www.bioz.com/result/bsmai/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bsmai - by Bioz Stars, 2021-06
    95/100 stars

    Images

    1) Product Images from "Somatic Mosaicism in Menkes Disease Suggests Choroid Plexus-mediated Copper Transport to the Developing Brain"

    Article Title: Somatic Mosaicism in Menkes Disease Suggests Choroid Plexus-mediated Copper Transport to the Developing Brain

    Journal: American journal of medical genetics. Part A

    doi: 10.1002/ajmg.a.33632

    Analysis of mosaicism. A. Diagram of ATP7A exon 15 region that was amplified from genomic DNA from the mosaic Menkes disease patient and a normal control, showing position of a novel restriction site ( BsmAI ) created by the P1001L mutation. The 294 bp
    Figure Legend Snippet: Analysis of mosaicism. A. Diagram of ATP7A exon 15 region that was amplified from genomic DNA from the mosaic Menkes disease patient and a normal control, showing position of a novel restriction site ( BsmAI ) created by the P1001L mutation. The 294 bp

    Techniques Used: Amplification, Mutagenesis

    2) Product Images from "Pathogenicity of two COQ7 mutations and responses to 2,4‐dihydroxybenzoate bypass treatment"

    Article Title: Pathogenicity of two COQ7 mutations and responses to 2,4‐dihydroxybenzoate bypass treatment

    Journal: Journal of Cellular and Molecular Medicine

    doi: 10.1111/jcmm.13154

    COQ7 gene variants and a mitochondrial DNA mutation identified in the patient under study. ( A ) COQ7 gene variants. Sequencing chromatograms are shown for the c.308C > T and c.332T > C variants detected in COQ7 in the proband reported in this study, with the wild‐type sequence at the top. The chromatograms also show that the patient is homozygous for both the c.308C > T [p. Thr103Met] polymorphism and the c.332T > C [p.Leu111Pro] mutation. ( B ) Chromatograms showing a partial sequence of the mitochondria 12S rRNA gene in a normal control, the L111P patient reported in this study or the previously reported COQ7 patient carrying the V141E mutation. Both patients and the normal control used in this study carry the m.1438A > G polymorphism. The L111P patient, in addition, carries the m.1555A > G mutation. Black arrows denote altered nucleotides. ( C ) RFLP of mitochondrial DNA. Shown is an agarose gel electrophoresis of PCR products from the mitochondrial 12S rRNA gene digested by BsmAI. Wild‐type control: skin fibroblasts from a healthy unrelated individual; L111P: the proband reported in this study; V141E: COQ7 patient previously reported in 19 . The PCR product (643 bp) without the m.1555A > G mutation was cleaved in two fragments of 409 and 234 bp, which was the case for the wild‐type control and the V141E patient. The PCR product from the L111P patient's 12S rRNA gene was not cut by BsmAI, as the m.1555A > G mutation abolishes the BsmAI restriction site. Moreover, only a single band corresponding to the mutated fragment was seen in the sample from the L111P patient. This, together with the sequencing chromatogram, demonstrates homoplasmy for the m.1555A > G mutation.
    Figure Legend Snippet: COQ7 gene variants and a mitochondrial DNA mutation identified in the patient under study. ( A ) COQ7 gene variants. Sequencing chromatograms are shown for the c.308C > T and c.332T > C variants detected in COQ7 in the proband reported in this study, with the wild‐type sequence at the top. The chromatograms also show that the patient is homozygous for both the c.308C > T [p. Thr103Met] polymorphism and the c.332T > C [p.Leu111Pro] mutation. ( B ) Chromatograms showing a partial sequence of the mitochondria 12S rRNA gene in a normal control, the L111P patient reported in this study or the previously reported COQ7 patient carrying the V141E mutation. Both patients and the normal control used in this study carry the m.1438A > G polymorphism. The L111P patient, in addition, carries the m.1555A > G mutation. Black arrows denote altered nucleotides. ( C ) RFLP of mitochondrial DNA. Shown is an agarose gel electrophoresis of PCR products from the mitochondrial 12S rRNA gene digested by BsmAI. Wild‐type control: skin fibroblasts from a healthy unrelated individual; L111P: the proband reported in this study; V141E: COQ7 patient previously reported in 19 . The PCR product (643 bp) without the m.1555A > G mutation was cleaved in two fragments of 409 and 234 bp, which was the case for the wild‐type control and the V141E patient. The PCR product from the L111P patient's 12S rRNA gene was not cut by BsmAI, as the m.1555A > G mutation abolishes the BsmAI restriction site. Moreover, only a single band corresponding to the mutated fragment was seen in the sample from the L111P patient. This, together with the sequencing chromatogram, demonstrates homoplasmy for the m.1555A > G mutation.

    Techniques Used: Mutagenesis, Sequencing, Agarose Gel Electrophoresis, Polymerase Chain Reaction

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Rapid Identification of Candida dubliniensis Using a Species-Specific Molecular Beacon
    Article Snippet: The PCR mixture was subjected to PCR with the following cycling conditions: 95°C for 10 min, followed by 40 cycles of 95°C for 30 s, 58°C for 1 min, and 72°C for 1 min and a final step of 72°C for 5 min. PCR-amplified products were purified with the Qiaquick DNA Purification Kit (Qiagen, Valencia, Calif.). .. The PCR amplification products (∼500 ng) were digested with 1 μl of restriction enzymes Nsp BII ( C. albicans ITS2 specific) (AP Biotech, Piscataway, N.J.) and Bsm AI ( C. dubliniensis ITS2 specific) (New England Biolabs, Beverly, Mass.) for 1 h and were analyzed by gel electrophoresis in a 1.5% agarose gel. ..

    Article Title: Slow-Channel Myasthenic Syndrome Caused By Enhanced Activation, Desensitization, and Agonist Binding Affinity Attributable to Mutation in the M2 Domain of the Acetylcholine Receptor α Subunit
    Article Snippet: The mismatched nucleotide “g,” which introduces a mutation four nucleotides downstream from the αG745T (αV249F) mutant site, enables Bsm AI to differentiate among PCR products with or without the αV249F: Bsm AI yields 134 and 23 bp fragments from the PCR product lacking αV249F mutation, and a 157 bp fragment from the PCR product harboring αV249F. .. The PCR product was digested with Bsm AI (New England Biolabs, Beverly, MA). ..

    Article Title: c-erbB-2 is not a major factor in the development of colorectal cancer
    Article Snippet: PCR was carried out in a Hybaid Omnigene thermocycler (Hybaid, Middlesex, UK), and consisted of an initial heating at 95°C for 12 min to activate the enzyme, followed by 35 cycles of denaturation at 94°C for 30 s, annealing at 62°C for 30 s and extension at 72°C for 30 s, with a final extension at 72°C for 7 min. Genomic DNA known to be homozygous for the Val allele was included as a positive control, while DNA was omitted from negative control samples. .. PCR product (10 μl) was digested with 5 U Bsm AI (New England BioLabs, Hertfordshire, UK) at 55°C for 2 h, and visualized by electrophoresis on 2.5% agarose containing 0.5 μg ml−1 ethidium bromide. .. The 148 bp PCR product generated was cut by Bsm AI into fragments of 122 and 26 bp if the Ile allele was present, whereas the product from the Val allele was cut to produce fragments of 90, 32 and 26 bp ( ).

    Article Title: Molecular and Clinical Characterization of the Variable Phenotype in Korean Families with Hearing Loss Associated with the Mitochondrial A1555G Mutation
    Article Snippet: PCR amplification of the mitochondrial 12S rRNA gene was performed using the following primers: forward, 5′- tggctttaacatatctgaacaca-3′, and reverse, 5′-ctcctaagtgtaagttgggtgct-3′. .. For the identification of the A1555G mutation, the PCR products were analyzed using PCR-RFLP with Bsm AI (New England Biolabs, Ipswich, MA, USA) . .. To confirm the A1555G mutation, the PCR products were purified with the Exo-SAP enzyme (USB, Cleveland, OH, USA) and analyzed through direct sequencing on an ABI 3130 Genetic Analyzer (Applied Biosystems Corps., Foster City, CA, USA) using the Big-Dye Terminator Cycle Sequencing Kit (Applied Biosystems Corps., Foster City, CA, USA).

    Article Title: The lss Supernodulation Mutant of Medicago truncatula Reduces Expression of the SUNN Gene 1 Gene 1 [OA]
    Article Snippet: .. Following amplification, the PCR products were digested with Bsm AI (New England Biolabs) at 55°C for 4 h, which cuts only the sunn-2 allele, or Hae III (New England Biolabs) at 37°C for 4 h, which cuts only the sunn-1 allele. .. Following digestion, the samples were run on a 1% agarose gel and stained with ethidium bromide.

    Article Title: Somatic Mosaicism in Menkes Disease Suggests Choroid Plexus-mediated Copper Transport to the Developing Brain
    Article Snippet: The polymerase chain reaction (PCR) was used to amplify genomic DNA isolated from whole blood and fibroblasts using primers (forward: 5′-AATACGATTTGCTTTCCAAGCCTC-3′; reverse: 5′-AGCACCTACTCCTGTACCC-3′) specific for ATP7A exon 15. .. The 294 bp PCR products generated were purified using a DNA isolation kit (QIAGEN, Valencia, CA, USA), digested with BsmAI (NEB, Beverly, MA) and electrophoresed through 2% agarose. ..

    Article Title: Pathogenicity of two COQ7 mutations and responses to 2,4‐dihydroxybenzoate bypass treatment
    Article Snippet: .. The 643‐bp PCR product was subjected to sequencing using the same primers as well as restriction fragment length polymorphism (RFLP) analysis using BsmAI (New England Biolabs). .. The digestion of PCR products from samples without the 1555A > G mutation produces two fragments of 409 and 234 bp due to the recognition of the restriction site of BsmAI.

    Amplification:

    Article Title: Rapid Identification of Candida dubliniensis Using a Species-Specific Molecular Beacon
    Article Snippet: The PCR mixture was subjected to PCR with the following cycling conditions: 95°C for 10 min, followed by 40 cycles of 95°C for 30 s, 58°C for 1 min, and 72°C for 1 min and a final step of 72°C for 5 min. PCR-amplified products were purified with the Qiaquick DNA Purification Kit (Qiagen, Valencia, Calif.). .. The PCR amplification products (∼500 ng) were digested with 1 μl of restriction enzymes Nsp BII ( C. albicans ITS2 specific) (AP Biotech, Piscataway, N.J.) and Bsm AI ( C. dubliniensis ITS2 specific) (New England Biolabs, Beverly, Mass.) for 1 h and were analyzed by gel electrophoresis in a 1.5% agarose gel. ..

    Article Title: The lss Supernodulation Mutant of Medicago truncatula Reduces Expression of the SUNN Gene 1 Gene 1 [OA]
    Article Snippet: .. Following amplification, the PCR products were digested with Bsm AI (New England Biolabs) at 55°C for 4 h, which cuts only the sunn-2 allele, or Hae III (New England Biolabs) at 37°C for 4 h, which cuts only the sunn-1 allele. .. Following digestion, the samples were run on a 1% agarose gel and stained with ethidium bromide.

    Nucleic Acid Electrophoresis:

    Article Title: Rapid Identification of Candida dubliniensis Using a Species-Specific Molecular Beacon
    Article Snippet: The PCR mixture was subjected to PCR with the following cycling conditions: 95°C for 10 min, followed by 40 cycles of 95°C for 30 s, 58°C for 1 min, and 72°C for 1 min and a final step of 72°C for 5 min. PCR-amplified products were purified with the Qiaquick DNA Purification Kit (Qiagen, Valencia, Calif.). .. The PCR amplification products (∼500 ng) were digested with 1 μl of restriction enzymes Nsp BII ( C. albicans ITS2 specific) (AP Biotech, Piscataway, N.J.) and Bsm AI ( C. dubliniensis ITS2 specific) (New England Biolabs, Beverly, Mass.) for 1 h and were analyzed by gel electrophoresis in a 1.5% agarose gel. ..

    Agarose Gel Electrophoresis:

    Article Title: Rapid Identification of Candida dubliniensis Using a Species-Specific Molecular Beacon
    Article Snippet: The PCR mixture was subjected to PCR with the following cycling conditions: 95°C for 10 min, followed by 40 cycles of 95°C for 30 s, 58°C for 1 min, and 72°C for 1 min and a final step of 72°C for 5 min. PCR-amplified products were purified with the Qiaquick DNA Purification Kit (Qiagen, Valencia, Calif.). .. The PCR amplification products (∼500 ng) were digested with 1 μl of restriction enzymes Nsp BII ( C. albicans ITS2 specific) (AP Biotech, Piscataway, N.J.) and Bsm AI ( C. dubliniensis ITS2 specific) (New England Biolabs, Beverly, Mass.) for 1 h and were analyzed by gel electrophoresis in a 1.5% agarose gel. ..

    Electrophoresis:

    Article Title: c-erbB-2 is not a major factor in the development of colorectal cancer
    Article Snippet: PCR was carried out in a Hybaid Omnigene thermocycler (Hybaid, Middlesex, UK), and consisted of an initial heating at 95°C for 12 min to activate the enzyme, followed by 35 cycles of denaturation at 94°C for 30 s, annealing at 62°C for 30 s and extension at 72°C for 30 s, with a final extension at 72°C for 7 min. Genomic DNA known to be homozygous for the Val allele was included as a positive control, while DNA was omitted from negative control samples. .. PCR product (10 μl) was digested with 5 U Bsm AI (New England BioLabs, Hertfordshire, UK) at 55°C for 2 h, and visualized by electrophoresis on 2.5% agarose containing 0.5 μg ml−1 ethidium bromide. .. The 148 bp PCR product generated was cut by Bsm AI into fragments of 122 and 26 bp if the Ile allele was present, whereas the product from the Val allele was cut to produce fragments of 90, 32 and 26 bp ( ).

    Mutagenesis:

    Article Title: Molecular and Clinical Characterization of the Variable Phenotype in Korean Families with Hearing Loss Associated with the Mitochondrial A1555G Mutation
    Article Snippet: PCR amplification of the mitochondrial 12S rRNA gene was performed using the following primers: forward, 5′- tggctttaacatatctgaacaca-3′, and reverse, 5′-ctcctaagtgtaagttgggtgct-3′. .. For the identification of the A1555G mutation, the PCR products were analyzed using PCR-RFLP with Bsm AI (New England Biolabs, Ipswich, MA, USA) . .. To confirm the A1555G mutation, the PCR products were purified with the Exo-SAP enzyme (USB, Cleveland, OH, USA) and analyzed through direct sequencing on an ABI 3130 Genetic Analyzer (Applied Biosystems Corps., Foster City, CA, USA) using the Big-Dye Terminator Cycle Sequencing Kit (Applied Biosystems Corps., Foster City, CA, USA).

    Generated:

    Article Title: Somatic Mosaicism in Menkes Disease Suggests Choroid Plexus-mediated Copper Transport to the Developing Brain
    Article Snippet: The polymerase chain reaction (PCR) was used to amplify genomic DNA isolated from whole blood and fibroblasts using primers (forward: 5′-AATACGATTTGCTTTCCAAGCCTC-3′; reverse: 5′-AGCACCTACTCCTGTACCC-3′) specific for ATP7A exon 15. .. The 294 bp PCR products generated were purified using a DNA isolation kit (QIAGEN, Valencia, CA, USA), digested with BsmAI (NEB, Beverly, MA) and electrophoresed through 2% agarose. ..

    Purification:

    Article Title: Somatic Mosaicism in Menkes Disease Suggests Choroid Plexus-mediated Copper Transport to the Developing Brain
    Article Snippet: The polymerase chain reaction (PCR) was used to amplify genomic DNA isolated from whole blood and fibroblasts using primers (forward: 5′-AATACGATTTGCTTTCCAAGCCTC-3′; reverse: 5′-AGCACCTACTCCTGTACCC-3′) specific for ATP7A exon 15. .. The 294 bp PCR products generated were purified using a DNA isolation kit (QIAGEN, Valencia, CA, USA), digested with BsmAI (NEB, Beverly, MA) and electrophoresed through 2% agarose. ..

    DNA Extraction:

    Article Title: Somatic Mosaicism in Menkes Disease Suggests Choroid Plexus-mediated Copper Transport to the Developing Brain
    Article Snippet: The polymerase chain reaction (PCR) was used to amplify genomic DNA isolated from whole blood and fibroblasts using primers (forward: 5′-AATACGATTTGCTTTCCAAGCCTC-3′; reverse: 5′-AGCACCTACTCCTGTACCC-3′) specific for ATP7A exon 15. .. The 294 bp PCR products generated were purified using a DNA isolation kit (QIAGEN, Valencia, CA, USA), digested with BsmAI (NEB, Beverly, MA) and electrophoresed through 2% agarose. ..

    Sequencing:

    Article Title: Pathogenicity of two COQ7 mutations and responses to 2,4‐dihydroxybenzoate bypass treatment
    Article Snippet: .. The 643‐bp PCR product was subjected to sequencing using the same primers as well as restriction fragment length polymorphism (RFLP) analysis using BsmAI (New England Biolabs). .. The digestion of PCR products from samples without the 1555A > G mutation produces two fragments of 409 and 234 bp due to the recognition of the restriction site of BsmAI.

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    New England Biolabs bsmai
    Analysis of mosaicism. A. Diagram of ATP7A exon 15 region that was amplified from genomic <t>DNA</t> from the mosaic Menkes disease patient and a normal control, showing position of a novel restriction site ( <t>BsmAI</t> ) created by the P1001L mutation. The 294 bp
    Bsmai, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bsmai/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bsmai - by Bioz Stars, 2021-06
    95/100 stars
    		  Buy from Supplier

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    Analysis of mosaicism. A. Diagram of ATP7A exon 15 region that was amplified from genomic DNA from the mosaic Menkes disease patient and a normal control, showing position of a novel restriction site ( BsmAI ) created by the P1001L mutation. The 294 bp

    Journal: American journal of medical genetics. Part A

    Article Title: Somatic Mosaicism in Menkes Disease Suggests Choroid Plexus-mediated Copper Transport to the Developing Brain

    doi: 10.1002/ajmg.a.33632

    Figure Lengend Snippet: Analysis of mosaicism. A. Diagram of ATP7A exon 15 region that was amplified from genomic DNA from the mosaic Menkes disease patient and a normal control, showing position of a novel restriction site ( BsmAI ) created by the P1001L mutation. The 294 bp

    Article Snippet: The 294 bp PCR products generated were purified using a DNA isolation kit (QIAGEN, Valencia, CA, USA), digested with BsmAI (NEB, Beverly, MA) and electrophoresed through 2% agarose.

    Techniques: Amplification, Mutagenesis

    Origin of expressed SUNN in sunn LSS/ lss SUNN plants. A, Diagram of mutation and PCR product locations and restriction sites used in B. H indicates a Hae III restriction site, while B indicates a Bsm AI site; within the PCR product, differential digestion

    Journal: Plant Physiology

    Article Title: The lss Supernodulation Mutant of Medicago truncatula Reduces Expression of the SUNN Gene 1 Gene 1 [OA]

    doi: 10.1104/pp.110.164889

    Figure Lengend Snippet: Origin of expressed SUNN in sunn LSS/ lss SUNN plants. A, Diagram of mutation and PCR product locations and restriction sites used in B. H indicates a Hae III restriction site, while B indicates a Bsm AI site; within the PCR product, differential digestion

    Article Snippet: Following amplification, the PCR products were digested with Bsm AI (New England Biolabs) at 55°C for 4 h, which cuts only the sunn-2 allele, or Hae III (New England Biolabs) at 37°C for 4 h, which cuts only the sunn-1 allele.

    Techniques: Mutagenesis, Polymerase Chain Reaction

    Identification and analysis of mutation in the AChR α subunit. A , Automated sequencing of α exon 7 around codon 249 in a control and the propositus. In the propositus, both G and T nucleotides are present at position 745 ( arrow ), indicating a heterozygous G→T transversion. This mutation changes codon 249 from a GTC for valine to a TTC for phenylalanine. B , Allele-specific PCR and mutagenic PCR plus Bsm AI analysis of genomic DNA in the propositus’ family. Both wild-type and mutant-allele-specific primers amplify an expected 168 bp fragment in propositus and his father, but only the wild-type primer amplifies the expected fragment in the other family members. On Bsm AI analysis after mutagenic PCR, the wild-type allele gives rise to a 134 bp fragment ( open arrowhead ) and a 23 bp fragment (not shown); the mutant allele yields an undigested 157 bp fragment ( closed arrowhead ). Both wild-type and mutant fragments appear in the propositus, but only the wild-type fragment appears in other family members. The incongruity between allele-specific PCR and restriction analysis in the father is attributable to the father being a mosaic for αV249F (see text). The arrow indicates propositus. C , Multiple alignment of AChR M2 membrane-spanning domains. The boxes enclose the conserved valine residues in human AChR subunits and in AChR α subunits of other species. The mutant phenylalanine is shown at the bottom.

    Journal: The Journal of Neuroscience

    Article Title: Slow-Channel Myasthenic Syndrome Caused By Enhanced Activation, Desensitization, and Agonist Binding Affinity Attributable to Mutation in the M2 Domain of the Acetylcholine Receptor α Subunit

    doi: 10.1523/JNEUROSCI.17-15-05651.1997

    Figure Lengend Snippet: Identification and analysis of mutation in the AChR α subunit. A , Automated sequencing of α exon 7 around codon 249 in a control and the propositus. In the propositus, both G and T nucleotides are present at position 745 ( arrow ), indicating a heterozygous G→T transversion. This mutation changes codon 249 from a GTC for valine to a TTC for phenylalanine. B , Allele-specific PCR and mutagenic PCR plus Bsm AI analysis of genomic DNA in the propositus’ family. Both wild-type and mutant-allele-specific primers amplify an expected 168 bp fragment in propositus and his father, but only the wild-type primer amplifies the expected fragment in the other family members. On Bsm AI analysis after mutagenic PCR, the wild-type allele gives rise to a 134 bp fragment ( open arrowhead ) and a 23 bp fragment (not shown); the mutant allele yields an undigested 157 bp fragment ( closed arrowhead ). Both wild-type and mutant fragments appear in the propositus, but only the wild-type fragment appears in other family members. The incongruity between allele-specific PCR and restriction analysis in the father is attributable to the father being a mosaic for αV249F (see text). The arrow indicates propositus. C , Multiple alignment of AChR M2 membrane-spanning domains. The boxes enclose the conserved valine residues in human AChR subunits and in AChR α subunits of other species. The mutant phenylalanine is shown at the bottom.

    Article Snippet: The PCR product was digested with Bsm AI (New England Biolabs, Beverly, MA).

    Techniques: Mutagenesis, Sequencing, Polymerase Chain Reaction

    The ITS2 region was PCR amplified with universal fungal primers ITS3 and ITS4, and the products were digested with restriction enzymes Bsm AI ( C. dubliniensis specific) (A) and Nsp BII ( C. albicans specific) (B). The restriction fragments were run on a 1.2% agarose gel and stained with GelStar (FMC Bioproducts). ∗, C. dubliniensis strains.

    Journal: Journal of Clinical Microbiology

    Article Title: Rapid Identification of Candida dubliniensis Using a Species-Specific Molecular Beacon

    doi:

    Figure Lengend Snippet: The ITS2 region was PCR amplified with universal fungal primers ITS3 and ITS4, and the products were digested with restriction enzymes Bsm AI ( C. dubliniensis specific) (A) and Nsp BII ( C. albicans specific) (B). The restriction fragments were run on a 1.2% agarose gel and stained with GelStar (FMC Bioproducts). ∗, C. dubliniensis strains.

    Article Snippet: The PCR amplification products (∼500 ng) were digested with 1 μl of restriction enzymes Nsp BII ( C. albicans ITS2 specific) (AP Biotech, Piscataway, N.J.) and Bsm AI ( C. dubliniensis ITS2 specific) (New England Biolabs, Beverly, Mass.) for 1 h and were analyzed by gel electrophoresis in a 1.5% agarose gel.

    Techniques: Polymerase Chain Reaction, Amplification, Agarose Gel Electrophoresis, Staining