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    BsrI
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    BsrI 5 000 units
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    Restriction Enzymes
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    New England Biolabs bsr i
    BsrI
    BsrI 5 000 units
    https://www.bioz.com/result/bsr i/product/New England Biolabs
    Average 98 stars, based on 16711 article reviews
    Price from $9.99 to $1999.99
    bsr i - by Bioz Stars, 2020-09
    98/100 stars

    Images

    1) Product Images from "The distribution of insertionally polymorphic endogenous retroviruses in breast cancer patients and cancer-free controls"

    Article Title: The distribution of insertionally polymorphic endogenous retroviruses in breast cancer patients and cancer-free controls

    Journal: Retrovirology

    doi: 10.1186/s12977-014-0062-3

    Distribution of polymorphic HML-2 proviruses in breast cancer cases and controls. A . Comparative schematic representing the in silico -predicted sizes for HML-2 containing fragments following Bsr I digestion and detected by the K-seq probe within the Hg19 genome build. Asterisks at left indicate the confirmed polymorphic proviruses, whose distribution coincides exactly between unblot banding patterns and PCR data. B . CPSII samples were sorted by case/control status (n = 25 each) and Bsr I digested WGA-DNA from each group was separated by gel electrophoresis and probed with the 32 P-radiolabeled K-seq oligonucleotide. HML-2 junction fragments were visualized following exposure to film, and polymorphic insertions inferred by variable banding patterns among samples. C . Results from PCR analysis of known polymorphic proviruses for direct comparison of described polymorphic elements, where ‘+’ indicates the confirmed presence of the tested provirus. Novel polymorphic fragments whose identity could not be inferred by comparison to PCR analysis or in silico predictions, have been indicated with arrows at right. Asterisks (at right) are used to indicate the observed fragment sizes of polymorphic elements detected in ≤5% individuals screened here.
    Figure Legend Snippet: Distribution of polymorphic HML-2 proviruses in breast cancer cases and controls. A . Comparative schematic representing the in silico -predicted sizes for HML-2 containing fragments following Bsr I digestion and detected by the K-seq probe within the Hg19 genome build. Asterisks at left indicate the confirmed polymorphic proviruses, whose distribution coincides exactly between unblot banding patterns and PCR data. B . CPSII samples were sorted by case/control status (n = 25 each) and Bsr I digested WGA-DNA from each group was separated by gel electrophoresis and probed with the 32 P-radiolabeled K-seq oligonucleotide. HML-2 junction fragments were visualized following exposure to film, and polymorphic insertions inferred by variable banding patterns among samples. C . Results from PCR analysis of known polymorphic proviruses for direct comparison of described polymorphic elements, where ‘+’ indicates the confirmed presence of the tested provirus. Novel polymorphic fragments whose identity could not be inferred by comparison to PCR analysis or in silico predictions, have been indicated with arrows at right. Asterisks (at right) are used to indicate the observed fragment sizes of polymorphic elements detected in ≤5% individuals screened here.

    Techniques Used: In Silico, Polymerase Chain Reaction, Whole Genome Amplification, Nucleic Acid Electrophoresis

    2) Product Images from "Helicobacter winghamensis sp. nov., a Novel Helicobacter sp. Isolated from Patients with Gastroenteritis"

    Article Title: Helicobacter winghamensis sp. nov., a Novel Helicobacter sp. Isolated from Patients with Gastroenteritis

    Journal: Journal of Clinical Microbiology

    doi: 10.1128/JCM.39.7.2412-2417.2001

    16S rRNA PCR-RFLP patterns for intestinal helicobacters generated using restriction enzyme Bsr I. Lane 1, H. bizzozeronii ; lane 2, H. canadensis ; lane 3, H. canis ; lane 4, H. cinaedi ; lane 5, H. fennelliae ; lane 6, H. pullorum ; lane 7, Helicobacter sp. flexispira taxon 8; lane 8, H. winghamensis ; lane M, 100-bp molecular weight marker (New England Biolabs)
    Figure Legend Snippet: 16S rRNA PCR-RFLP patterns for intestinal helicobacters generated using restriction enzyme Bsr I. Lane 1, H. bizzozeronii ; lane 2, H. canadensis ; lane 3, H. canis ; lane 4, H. cinaedi ; lane 5, H. fennelliae ; lane 6, H. pullorum ; lane 7, Helicobacter sp. flexispira taxon 8; lane 8, H. winghamensis ; lane M, 100-bp molecular weight marker (New England Biolabs)

    Techniques Used: Polymerase Chain Reaction, Generated, Molecular Weight, Marker

    3) Product Images from "Investigation of FOXP3 genetic variations at positions -2383 C/T and IVS9+459 T/C in southern Iranian patients with lung carcinoma"

    Article Title: Investigation of FOXP3 genetic variations at positions -2383 C/T and IVS9+459 T/C in southern Iranian patients with lung carcinoma

    Journal: Iranian Journal of Basic Medical Sciences

    doi:

    Genotyping of -2383 C/T in FOXP3 gene using polymerase chain reaction-restriction fragment length polymorphism technique with Bsr I ( Bse NI) enzyme, followed by the band detection on gel red-stained 3% agarose gel after electrophoresis. 1: pUC19 marker, 2: CC in females and C in males (184, 127, and77 bp), 3: CT (just in females) (261, 184, 127, and 77 bp), 4: TT in females and T in males (261 and 127 bp)
    Figure Legend Snippet: Genotyping of -2383 C/T in FOXP3 gene using polymerase chain reaction-restriction fragment length polymorphism technique with Bsr I ( Bse NI) enzyme, followed by the band detection on gel red-stained 3% agarose gel after electrophoresis. 1: pUC19 marker, 2: CC in females and C in males (184, 127, and77 bp), 3: CT (just in females) (261, 184, 127, and 77 bp), 4: TT in females and T in males (261 and 127 bp)

    Techniques Used: Polymerase Chain Reaction, Staining, Agarose Gel Electrophoresis, Electrophoresis, Marker

    4) Product Images from "Nucleotide sequence and variations of the bovine myocyte enhancer factor 2C (MEF2C) gene promoter in Bos Taurus cattle"

    Article Title: Nucleotide sequence and variations of the bovine myocyte enhancer factor 2C (MEF2C) gene promoter in Bos Taurus cattle

    Journal: Molecular Biology Reports

    doi: 10.1007/s11033-010-0226-8

    RFLP/MSSCP genotyping of the two nucleotide substitutions and two deletions in promoter of the bovine MEF2 C gene. a RFLP genotyping of the g. - 1606C > T with Bsr I nuclease; b MSSCP genotyping of the DelG at position - 1336_ - 1335 ; C MSSCP genotyping of the C/T transition at position - 818 ; D. MSSCP genotyping of the DelA at position - 613_ - 612
    Figure Legend Snippet: RFLP/MSSCP genotyping of the two nucleotide substitutions and two deletions in promoter of the bovine MEF2 C gene. a RFLP genotyping of the g. - 1606C > T with Bsr I nuclease; b MSSCP genotyping of the DelG at position - 1336_ - 1335 ; C MSSCP genotyping of the C/T transition at position - 818 ; D. MSSCP genotyping of the DelA at position - 613_ - 612

    Techniques Used:

    5) Product Images from "Comparison of the Complete DNA Sequences of the Oka Varicella Vaccine and Its Parental Virus"

    Article Title: Comparison of the Complete DNA Sequences of the Oka Varicella Vaccine and Its Parental Virus

    Journal: Journal of Virology

    doi: 10.1128/JVI.76.22.11447-11459.2002

    RFLP analysis of the PCR products from V-Oka, P-Oka, and 54 wild-type viruses. V-Oka (V) can be distinguished not only from P-Oka (P) but also from all wild-type viruses. The Kawaguchi strain (K) was used to represent the wild-type viruses by using Alu I (a), Bst XI (b), Acc II (c), Sfa NI (d), Sac II (e), Sma I (f), and Bsr I (g).
    Figure Legend Snippet: RFLP analysis of the PCR products from V-Oka, P-Oka, and 54 wild-type viruses. V-Oka (V) can be distinguished not only from P-Oka (P) but also from all wild-type viruses. The Kawaguchi strain (K) was used to represent the wild-type viruses by using Alu I (a), Bst XI (b), Acc II (c), Sfa NI (d), Sac II (e), Sma I (f), and Bsr I (g).

    Techniques Used: Polymerase Chain Reaction

    6) Product Images from "Sclera-related gene polymorphisms in high myopia"

    Article Title: Sclera-related gene polymorphisms in high myopia

    Journal: Molecular Vision

    doi:

    The PCR product of bFGF ( rs308395 ) polymorphism. The PCR product of βFGF2 - rs308395 polymorphism was digested with BsrI. The “ C ” allele was 241 bp and the “ G ” allele was 163 bp + 78 bp.
    Figure Legend Snippet: The PCR product of bFGF ( rs308395 ) polymorphism. The PCR product of βFGF2 - rs308395 polymorphism was digested with BsrI. The “ C ” allele was 241 bp and the “ G ” allele was 163 bp + 78 bp.

    Techniques Used: Polymerase Chain Reaction

    7) Product Images from "Discrimination of SHV ?-Lactamase Genes by Restriction Site Insertion-PCR"

    Article Title: Discrimination of SHV ?-Lactamase Genes by Restriction Site Insertion-PCR

    Journal: Antimicrobial Agents and Chemotherapy

    doi: 10.1128/AAC.45.7.2110-2114.2001

    Mutations of bla SHV genes demonstrated by RSI-PCR analysis with various restriction endonucleases. (a) Position 8 with Ssp I digestion; (b) position 179 with Hin fI digestion; (c) position 238 with Bsr I digestion. Lanes A to H, amplimers of bla SHV genes bla SHV-1 (A), bla SHV-2 (B), bla SHV-3 (C), bla SHV-4 (D), bla SHV-5 (E), bla SHV-6 (F), bla SHV-8 (G), and bla SHV-18 (H) after digestion; lane I, amplimer of bla SHV-1 gene before digestion; lane M, 100-bp ladder.
    Figure Legend Snippet: Mutations of bla SHV genes demonstrated by RSI-PCR analysis with various restriction endonucleases. (a) Position 8 with Ssp I digestion; (b) position 179 with Hin fI digestion; (c) position 238 with Bsr I digestion. Lanes A to H, amplimers of bla SHV genes bla SHV-1 (A), bla SHV-2 (B), bla SHV-3 (C), bla SHV-4 (D), bla SHV-5 (E), bla SHV-6 (F), bla SHV-8 (G), and bla SHV-18 (H) after digestion; lane I, amplimer of bla SHV-1 gene before digestion; lane M, 100-bp ladder.

    Techniques Used: Polymerase Chain Reaction

    8) Product Images from "Rapid Identification of Campylobacter, Arcobacter, and Helicobacter Isolates by PCR-Restriction Fragment Length Polymorphism Analysis of the 16S rRNA Gene"

    Article Title: Rapid Identification of Campylobacter, Arcobacter, and Helicobacter Isolates by PCR-Restriction Fragment Length Polymorphism Analysis of the 16S rRNA Gene

    Journal: Journal of Clinical Microbiology

    doi:

    (A) 16S PCR-RFLP patterns observed with Dde I. (B) 16S PCR-RFLP patterns observed with Taq I and Bsr I. Letter above each lane denotes pattern obtained. Lane M, 100-bp ladder (New England Biolabs). The numbers are molecular sizes in kilobases.
    Figure Legend Snippet: (A) 16S PCR-RFLP patterns observed with Dde I. (B) 16S PCR-RFLP patterns observed with Taq I and Bsr I. Letter above each lane denotes pattern obtained. Lane M, 100-bp ladder (New England Biolabs). The numbers are molecular sizes in kilobases.

    Techniques Used: Polymerase Chain Reaction

    9) Product Images from "A point mutation decouples the lipid transfer activities of microsomal triglyceride transfer protein"

    Article Title: A point mutation decouples the lipid transfer activities of microsomal triglyceride transfer protein

    Journal: PLoS Genetics

    doi: 10.1371/journal.pgen.1008941

    The stl and c655 mttp mutations have differential effects on growth and the accumulation of lipid in intestine and liver. (A) Representative images of male WT and mttp mutant fish at 12 weeks of age. (B) Representative images of H E stained intestine and liver from adult male WT and mttp mutant fish (7.5 mo), scale = 50 μm, * indicate goblet cells, arrows indicate representative lipid accumulation in enterocytes. (C–E) Intestine and liver tissue from adult male fish were extracted based on equal concentration of protein. Tissue lipid extracts from WT and mttp mutant fish were quantitated using an HPLC system coupled to a tandem mass spectrometer (LC-MS/MS) (n = 3; 1 fish per sample/genotype). (C) Heat maps represent fold-change from WT of over 1000 individual lipid species grouped into lipid classes (triacylglycerol [TG, n = 274], diacylglycerol [DG, n = 108], mo noacylglycerol [MG, n = 36], sphingomyelin [SM, n = 72], cholesterol ester [CE, n = 7], ceramides [Cer, n = 44], phospholipid [PL, n = 472], free fatty acid [FA, n = 27] and other lipids [O; including sterols, sphingosine, sulfatide, zymosteryl and wax esters, n = 10]). (D) Quantification of total intestinal and liver TG, DG, PL, and FA from mutant lines as expressed as a sum of lipid group (n = 3). For additional lipid groups, see S11 Fig . (E) The number of individual lipid species data from panel (C) that are statistically different from WT (adj. p
    Figure Legend Snippet: The stl and c655 mttp mutations have differential effects on growth and the accumulation of lipid in intestine and liver. (A) Representative images of male WT and mttp mutant fish at 12 weeks of age. (B) Representative images of H E stained intestine and liver from adult male WT and mttp mutant fish (7.5 mo), scale = 50 μm, * indicate goblet cells, arrows indicate representative lipid accumulation in enterocytes. (C–E) Intestine and liver tissue from adult male fish were extracted based on equal concentration of protein. Tissue lipid extracts from WT and mttp mutant fish were quantitated using an HPLC system coupled to a tandem mass spectrometer (LC-MS/MS) (n = 3; 1 fish per sample/genotype). (C) Heat maps represent fold-change from WT of over 1000 individual lipid species grouped into lipid classes (triacylglycerol [TG, n = 274], diacylglycerol [DG, n = 108], mo noacylglycerol [MG, n = 36], sphingomyelin [SM, n = 72], cholesterol ester [CE, n = 7], ceramides [Cer, n = 44], phospholipid [PL, n = 472], free fatty acid [FA, n = 27] and other lipids [O; including sterols, sphingosine, sulfatide, zymosteryl and wax esters, n = 10]). (D) Quantification of total intestinal and liver TG, DG, PL, and FA from mutant lines as expressed as a sum of lipid group (n = 3). For additional lipid groups, see S11 Fig . (E) The number of individual lipid species data from panel (C) that are statistically different from WT (adj. p

    Techniques Used: Mutagenesis, Fluorescence In Situ Hybridization, Staining, Concentration Assay, High Performance Liquid Chromatography, Mass Spectrometry, Liquid Chromatography with Mass Spectroscopy

    Structural analysis of MTP mutations. (A) Ribbon representation of the human MTP complex (PDB entry 6I7S) and the Zebrafish modeled structure. The positions of L475 and G863 in the Zebrafish structure are shown in space-filling representation. (B) Alignment of human MTP and zebrafish Mtp amino acid sequences surrounding the stl and c655 mutations. (C) Close-up view of the area surrounding L477 in the human MTP complex. The position of L477 (red) is highlighted. The conserved hydrogen bonds linking the helical domain to the tip of the C-sheet of the lipid-binding domain are shown as well as amino acids within 4Å of L477. (D) Close-up view of the area surrounding G865 in the human MTP complex. The position of G865 (yellow) and the PEG molecule (dark green) which occupies the lipid-binding site in the solved structure are shown in space-filling representation. The a’ domain of PDI (pink) in the complex occludes the lipid entry/exit site. (E) Close-up view showing the outer strand displacement in sheet A of the lipid-binding domain of the M subunit resulting from the G865V mutation. Asterisk indicates the wild-type backbone carbonyl of G865 hydrogen bonded to R461 of PDI. Panels C–E are colored as in panel A.
    Figure Legend Snippet: Structural analysis of MTP mutations. (A) Ribbon representation of the human MTP complex (PDB entry 6I7S) and the Zebrafish modeled structure. The positions of L475 and G863 in the Zebrafish structure are shown in space-filling representation. (B) Alignment of human MTP and zebrafish Mtp amino acid sequences surrounding the stl and c655 mutations. (C) Close-up view of the area surrounding L477 in the human MTP complex. The position of L477 (red) is highlighted. The conserved hydrogen bonds linking the helical domain to the tip of the C-sheet of the lipid-binding domain are shown as well as amino acids within 4Å of L477. (D) Close-up view of the area surrounding G865 in the human MTP complex. The position of G865 (yellow) and the PEG molecule (dark green) which occupies the lipid-binding site in the solved structure are shown in space-filling representation. The a’ domain of PDI (pink) in the complex occludes the lipid entry/exit site. (E) Close-up view showing the outer strand displacement in sheet A of the lipid-binding domain of the M subunit resulting from the G865V mutation. Asterisk indicates the wild-type backbone carbonyl of G865 hydrogen bonded to R461 of PDI. Panels C–E are colored as in panel A.

    Techniques Used: Binding Assay, Mutagenesis

    The c655 allele is a missense mutation in the M-subunit of microsomal triglyceride transfer protein. (A) Representative images of a wild-type zebrafish embryo, a homozygous mutant embryo carrying the previously described stalactite ( stl ) missense mutation in mttp , and a homozygous c655 mutant embryo at 3 days post fertilization (dpf); Scale = 500 μm. The dark/opaque yolk phenotype in embryos from c655 heterozygous in-crosses segregated with a Mendelian ratio consistent with a homozygous recessive mutation, mean +/- SD. For source data, see S4 File . (B) Euclidean distance mapping analysis plots produced by MMAPPR [ 51 ], showing the likely genomic region of the c655 mutation. Plot of the LOESS fit to the mapping scores (Euclidean Distance 4 ) across all 25 chromosomes (top) and expanded view of chromosome 1(GRCz10: CM002885.1) (bottom). Single nucleotide variants (SNVs) present in this 11 MB region in c655 mutant embryos were assessed for their effect on annotated genes using the Ensembl Variant Effect Predictor [ 52 ], including using the Sorting Intolerant from Tolerant algorithm (SIFT) [ 53 ], to predict the impact of changes on protein-coding sequence (tolerated or deleterious). We extracted variants that alter the protein-coding sequence as candidates for the causal mutation (223 variants in 64 genes, of which 42 are missense variants predicted to be deleterious; S1 File ). One of the SNVs linked to the c655 phenotype was a G > T missense mutation predicted to be deleterious in exon 18 of the microsomal triglyceride transfer protein gene (ENSDARG00000008637, Chr1:11,421,261 GRCz10, red arrow in B shows the position of the G > T missense mutation in mttp ). (C) Representative image of a trans-heterozygous mttp stl/c655 embryo; 3 dpf, scale = 500 μm. The dark/opaque yolk phenotype is present at expected ratios and genotyping confirms that only the embryos with opaque yolks are trans-heterozygous for the mttp alleles. (D) Depiction of the mttp gene structure highlighting the locations of the stl (L475P) (position 11431645 (GRCz10), transcript mtp-204 (ENSDART00000165753.2)) and c655 (G863V) missense alleles in exon 11 and 18, respectively. An additional SNV in mttp at position Chr1:11,421,300 GRCz10 (T > C) causing a missense mutation (M850T) was also identified in c655 mutants; however, this SNV was not predicted to be deleterious and has been previously noted in Ensembl.
    Figure Legend Snippet: The c655 allele is a missense mutation in the M-subunit of microsomal triglyceride transfer protein. (A) Representative images of a wild-type zebrafish embryo, a homozygous mutant embryo carrying the previously described stalactite ( stl ) missense mutation in mttp , and a homozygous c655 mutant embryo at 3 days post fertilization (dpf); Scale = 500 μm. The dark/opaque yolk phenotype in embryos from c655 heterozygous in-crosses segregated with a Mendelian ratio consistent with a homozygous recessive mutation, mean +/- SD. For source data, see S4 File . (B) Euclidean distance mapping analysis plots produced by MMAPPR [ 51 ], showing the likely genomic region of the c655 mutation. Plot of the LOESS fit to the mapping scores (Euclidean Distance 4 ) across all 25 chromosomes (top) and expanded view of chromosome 1(GRCz10: CM002885.1) (bottom). Single nucleotide variants (SNVs) present in this 11 MB region in c655 mutant embryos were assessed for their effect on annotated genes using the Ensembl Variant Effect Predictor [ 52 ], including using the Sorting Intolerant from Tolerant algorithm (SIFT) [ 53 ], to predict the impact of changes on protein-coding sequence (tolerated or deleterious). We extracted variants that alter the protein-coding sequence as candidates for the causal mutation (223 variants in 64 genes, of which 42 are missense variants predicted to be deleterious; S1 File ). One of the SNVs linked to the c655 phenotype was a G > T missense mutation predicted to be deleterious in exon 18 of the microsomal triglyceride transfer protein gene (ENSDARG00000008637, Chr1:11,421,261 GRCz10, red arrow in B shows the position of the G > T missense mutation in mttp ). (C) Representative image of a trans-heterozygous mttp stl/c655 embryo; 3 dpf, scale = 500 μm. The dark/opaque yolk phenotype is present at expected ratios and genotyping confirms that only the embryos with opaque yolks are trans-heterozygous for the mttp alleles. (D) Depiction of the mttp gene structure highlighting the locations of the stl (L475P) (position 11431645 (GRCz10), transcript mtp-204 (ENSDART00000165753.2)) and c655 (G863V) missense alleles in exon 11 and 18, respectively. An additional SNV in mttp at position Chr1:11,421,300 GRCz10 (T > C) causing a missense mutation (M850T) was also identified in c655 mutants; however, this SNV was not predicted to be deleterious and has been previously noted in Ensembl.

    Techniques Used: Mutagenesis, Produced, Variant Assay, Sequencing

    The c655 mutation disrupts TG transfer activity, but not PL transfer activity of the zebrafish Mtp complex. (A, B) COS-7 cells were first transfected with an expression vector for human APOB48 (5 μg), distributed equally in 6-well plates, and subsequently transfected with plasmids expressing either wild-type zebrafish mttp -FLAG, mttp stl -FLAG, mttp c655 -FLAG, or empty vector (pcDNA3) (3 μg). After 72 h, APOB48 was measured via ELISA in media (A) or in the cell (B). Data are representative of 7 independent experiments (each data point is the mean of three technical replicates), mean +/- SD, One-Way ANOVA with Bonferroni post-hoc tests, * p
    Figure Legend Snippet: The c655 mutation disrupts TG transfer activity, but not PL transfer activity of the zebrafish Mtp complex. (A, B) COS-7 cells were first transfected with an expression vector for human APOB48 (5 μg), distributed equally in 6-well plates, and subsequently transfected with plasmids expressing either wild-type zebrafish mttp -FLAG, mttp stl -FLAG, mttp c655 -FLAG, or empty vector (pcDNA3) (3 μg). After 72 h, APOB48 was measured via ELISA in media (A) or in the cell (B). Data are representative of 7 independent experiments (each data point is the mean of three technical replicates), mean +/- SD, One-Way ANOVA with Bonferroni post-hoc tests, * p

    Techniques Used: Mutagenesis, Activity Assay, Transfection, Expressing, Plasmid Preparation, Enzyme-linked Immunosorbent Assay

    The opaque yolk phenotype results from the accumulation of aberrant cytoplasmic lipid droplets in the yolk syncytial layer. (A) (Top) Cartoon depicting the cross-sectional view of a 4 dpf zebrafish embryo. The YSL surrounds the yolk mass and serves as the embryonic digestive organ. The dashed box indicates the view expanded in the bottom panel and in panel B. (Bottom) Stored yolk lipids undergo lipolysis in yolk platelets (YP), presumably releasing free fatty acids into the YSL. These fatty acids are re-esterified in the ER bilayer to form TG, PL, and cholesterol esters. The lipids are packaged into B-lps in the ER with the help of Mtp and are likely further processed in the Golgi before being secreted into the perivitelline space (PS) and then circulation. (B) Representative transmission electron micrographs of the yolk and YSL from wild-type and mttp mutants; dashed lines delineate the YSL region, mt = mitochondria, scale = 10 μm. (C) Quantification of lipid droplet size in mttp mutants, n ≥ 700 lipid droplets in 2 fish per genotype; mean +/- SD. (D) Quantification of the number of lipid droplets per YSL area, n = 7–9 YSL regions per genotype (3–5 regions per fish, 2 fish per genotype); mean +/- SD, Kruskall-Wallis with Dunn’s Multiple Comparison test, vs. mttp c655/c655 , * p
    Figure Legend Snippet: The opaque yolk phenotype results from the accumulation of aberrant cytoplasmic lipid droplets in the yolk syncytial layer. (A) (Top) Cartoon depicting the cross-sectional view of a 4 dpf zebrafish embryo. The YSL surrounds the yolk mass and serves as the embryonic digestive organ. The dashed box indicates the view expanded in the bottom panel and in panel B. (Bottom) Stored yolk lipids undergo lipolysis in yolk platelets (YP), presumably releasing free fatty acids into the YSL. These fatty acids are re-esterified in the ER bilayer to form TG, PL, and cholesterol esters. The lipids are packaged into B-lps in the ER with the help of Mtp and are likely further processed in the Golgi before being secreted into the perivitelline space (PS) and then circulation. (B) Representative transmission electron micrographs of the yolk and YSL from wild-type and mttp mutants; dashed lines delineate the YSL region, mt = mitochondria, scale = 10 μm. (C) Quantification of lipid droplet size in mttp mutants, n ≥ 700 lipid droplets in 2 fish per genotype; mean +/- SD. (D) Quantification of the number of lipid droplets per YSL area, n = 7–9 YSL regions per genotype (3–5 regions per fish, 2 fish per genotype); mean +/- SD, Kruskall-Wallis with Dunn’s Multiple Comparison test, vs. mttp c655/c655 , * p

    Techniques Used: Transmission Assay, Fluorescence In Situ Hybridization

    The corresponding c655 mutation in human MTTP disrupts TG transfer but not PL transfer activity. (A) Immunofluorescence in COS-7 cells expressing wild-type human MTTP-FLAG or human MTTP(G865V)-FLAG proteins using anti-FLAG (red) and anti-Calnexin (green) antibodies; scale = 25 μm. (B) Human MTP-FLAG proteins (WT and G865V) were immunoprecipitated from COS-7 cell lysate (400 μg protein) using the M2 flag antibody and immunoblots were probed for both FLAG and PDI. For input, 15 μg protein was used. (C, D) COS-7 cells were co-transfected with human APOB48 and either wild-type human MTTP -FLAG, MTTP (G865V)-FLAG or empty pcDNA3 plasmids. After 72 h, APOB48 was measured via ELISA in media (C) or in the cell (D). Data are representative of 7 independent experiments (each data point is the mean of three technical replicates), pcDNA3 control data is re-graphed from Fig 5A 5B (data for Figs 5A , 5B , 6C and 6D were generated together); mean +/- SD, One-Way ANOVA with Bonferroni post-hoc tests, * p
    Figure Legend Snippet: The corresponding c655 mutation in human MTTP disrupts TG transfer but not PL transfer activity. (A) Immunofluorescence in COS-7 cells expressing wild-type human MTTP-FLAG or human MTTP(G865V)-FLAG proteins using anti-FLAG (red) and anti-Calnexin (green) antibodies; scale = 25 μm. (B) Human MTP-FLAG proteins (WT and G865V) were immunoprecipitated from COS-7 cell lysate (400 μg protein) using the M2 flag antibody and immunoblots were probed for both FLAG and PDI. For input, 15 μg protein was used. (C, D) COS-7 cells were co-transfected with human APOB48 and either wild-type human MTTP -FLAG, MTTP (G865V)-FLAG or empty pcDNA3 plasmids. After 72 h, APOB48 was measured via ELISA in media (C) or in the cell (D). Data are representative of 7 independent experiments (each data point is the mean of three technical replicates), pcDNA3 control data is re-graphed from Fig 5A 5B (data for Figs 5A , 5B , 6C and 6D were generated together); mean +/- SD, One-Way ANOVA with Bonferroni post-hoc tests, * p

    Techniques Used: Mutagenesis, Activity Assay, Immunofluorescence, Expressing, Immunoprecipitation, Western Blot, Transfection, Enzyme-linked Immunosorbent Assay, Generated

    The c655 mutation supports secretion of small LDL-sized lipoproteins in vivo . (A) LipoGlo fish express the NanoLuc luciferase enzyme as a C-terminal fusion on ApoBb.1 as a result of TALEN-based genomic engineering [ 48 ]. (B) LipoGlo signal (RLU: relative luminescence units) in WT, mttp stl/stl , and mttp c655/c655 fish throughout embryonic development (2–6 dpf). Results represent pooled data from 3 independent experiments, n = 22–34 fish/genotype/time-point. Significance was determined with a Robust ANOVA, Games-Howell post-hoc tests were performed to compare genotypes at each day of development, and p-values were adjusted to control for multiple comparisons, a = WT vs. mttp stl/stl , p
    Figure Legend Snippet: The c655 mutation supports secretion of small LDL-sized lipoproteins in vivo . (A) LipoGlo fish express the NanoLuc luciferase enzyme as a C-terminal fusion on ApoBb.1 as a result of TALEN-based genomic engineering [ 48 ]. (B) LipoGlo signal (RLU: relative luminescence units) in WT, mttp stl/stl , and mttp c655/c655 fish throughout embryonic development (2–6 dpf). Results represent pooled data from 3 independent experiments, n = 22–34 fish/genotype/time-point. Significance was determined with a Robust ANOVA, Games-Howell post-hoc tests were performed to compare genotypes at each day of development, and p-values were adjusted to control for multiple comparisons, a = WT vs. mttp stl/stl , p

    Techniques Used: Mutagenesis, In Vivo, Fluorescence In Situ Hybridization, Luciferase

    10) Product Images from "Association between FSHR polymorphisms and polycystic ovary syndrome among Chinese women in north China"

    Article Title: Association between FSHR polymorphisms and polycystic ovary syndrome among Chinese women in north China

    Journal: Journal of Assisted Reproduction and Genetics

    doi: 10.1007/s10815-013-0166-z

    a Electrophoretogram of DNA fragments for Ser680Asn polymorphism after digestion with Bsr I. Homozygote A/A was shown by the band of 520 bp. Homozygote G/G was shown by the bands of 413 bp and 107 bp. Heterozygote A/G was shown by the bands of 520 bp, 413 bp and 107 bp. b DNA sequencing of the Ser680Asn polymorphisms (AA, AG and GG) as indicated by arrows . The lane marked M is the marker of DNA. The lane marked P is the DNA of PCOS without enzyme digestion
    Figure Legend Snippet: a Electrophoretogram of DNA fragments for Ser680Asn polymorphism after digestion with Bsr I. Homozygote A/A was shown by the band of 520 bp. Homozygote G/G was shown by the bands of 413 bp and 107 bp. Heterozygote A/G was shown by the bands of 520 bp, 413 bp and 107 bp. b DNA sequencing of the Ser680Asn polymorphisms (AA, AG and GG) as indicated by arrows . The lane marked M is the marker of DNA. The lane marked P is the DNA of PCOS without enzyme digestion

    Techniques Used: DNA Sequencing, Marker

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Sclera-related gene polymorphisms in high myopia
    Article Snippet: .. The PCR product of bFGF ( rs308395 ) polymorphism was digested with BsrI (New England Biolabs). ..

    Article Title: Rapid Identification of Campylobacter, Arcobacter, and Helicobacter Isolates by PCR-Restriction Fragment Length Polymorphism Analysis of the 16S rRNA Gene
    Article Snippet: .. For restriction endonuclease digestion a 20-μl reaction mixture which included 10 μl of the PCR amplicon with 10 U of the restriction endonuclease Dde I (Boehringer-Mannheim, Indianapolis, Ind.), Taq I (Boehringer-Mannheim), or Bsr I (New England Biolabs, Inc., Beverly, Mass.) was employed, following conditions recommended by the respective manufacturers. .. Ten microliters of each digest was analyzed electrophoretically at 5 V/cm for 2 h with a 3% agarose gel in 0.5× Tris-borate-EDTA (ICN Biomedicals, Aurora, Ohio).

    Article Title: Nucleotide sequence and variations of the bovine myocyte enhancer factor 2C (MEF2C) gene promoter in Bos Taurus cattle
    Article Snippet: .. The 382-bp PCR product including P1 fragment was digested for 3 h with 5 U of Bsr I (New England Biolabs) restriction endonucleases. .. The restriction products were separated by electrophoresis in 3% agarose gel (Sigma-Aldrich, Munich, Germany) with ethidium bromide in 1× TBE buffer.

    Article Title: Discrimination of SHV ?-Lactamase Genes by Restriction Site Insertion-PCR
    Article Snippet: .. The PCR products were digested with restriction endonucleases according to the manufacturer's instructions ( Bsr I, Nru I, and Ssp I were purchased from New England Biolabs, Pst I and Hin fI were supplied from Promega and GibcoBRL, respectively). .. After digestion, the products were analysed by gel electrophoresis using 3% low-melting-point agarose (Metaphore; FMC Bio-Products, Flowgen, Staffordshire, United Kingdom).

    Electrophoresis:

    Article Title: Helicobacter winghamensis sp. nov., a Novel Helicobacter sp. Isolated from Patients with Gastroenteritis
    Article Snippet: .. The resulting amplicon was then digested with endonucleases Dde I and Bsr I (New England Biolabs, Mississauga, Ontario, Canada), and the resulting RFLP patterns were visualized after electrophoresis by ethidium bromide staining. .. For 16S rRNA gene sequencing, chromosomal DNA was first extracted from the isolates using either DNAzol (Molecular Research Center, Inc., Cincinnati, Ohio) or Integrated Separation Systems automated DNA extractor Autogen 540 according to the manufacturer's specifications (Enprotech, Natick, Mass.).

    Amplification:

    Article Title: Helicobacter winghamensis sp. nov., a Novel Helicobacter sp. Isolated from Patients with Gastroenteritis
    Article Snippet: .. The resulting amplicon was then digested with endonucleases Dde I and Bsr I (New England Biolabs, Mississauga, Ontario, Canada), and the resulting RFLP patterns were visualized after electrophoresis by ethidium bromide staining. .. For 16S rRNA gene sequencing, chromosomal DNA was first extracted from the isolates using either DNAzol (Molecular Research Center, Inc., Cincinnati, Ohio) or Integrated Separation Systems automated DNA extractor Autogen 540 according to the manufacturer's specifications (Enprotech, Natick, Mass.).

    Article Title: Rapid Identification of Campylobacter, Arcobacter, and Helicobacter Isolates by PCR-Restriction Fragment Length Polymorphism Analysis of the 16S rRNA Gene
    Article Snippet: .. For restriction endonuclease digestion a 20-μl reaction mixture which included 10 μl of the PCR amplicon with 10 U of the restriction endonuclease Dde I (Boehringer-Mannheim, Indianapolis, Ind.), Taq I (Boehringer-Mannheim), or Bsr I (New England Biolabs, Inc., Beverly, Mass.) was employed, following conditions recommended by the respective manufacturers. .. Ten microliters of each digest was analyzed electrophoretically at 5 V/cm for 2 h with a 3% agarose gel in 0.5× Tris-borate-EDTA (ICN Biomedicals, Aurora, Ohio).

    Article Title: Comparison of the Complete DNA Sequences of the Oka Varicella Vaccine and Its Parental Virus
    Article Snippet: .. Portions (3 μl) of the amplified products with three primer pairs—01-N12 and 01-R13, 50-N15 and 50-R17, or 60-N11 and 60-R12—were digested with 4 U of the restriction endonucleases Alu I (Takara Shuzo), Bst XI (Takara Shuzo), or Bsr I (New England Biolabs), respectively. .. The same volumes of the products obtained by using primer pair 60-N06 and 60-R06 and primer pair 60-N26 and 60-R28 were digested independently with 4 U of the enzymes Sfa NI (New England Biolabs)- Acc II (Takara Shuzo) and Sac II (New England Biolabs)- Sma I (New England Biolabs), respectively.

    Whole Genome Amplification:

    Article Title: The distribution of insertionally polymorphic endogenous retroviruses in breast cancer patients and cancer-free controls
    Article Snippet: .. For each sample, 15 μg of WGA DNA was digested with Bsr I (New England Biolabs) in a 100 μL volume and the digested products extracted and precipitated as described above. .. Products were resuspended in 20uL 0.25 x TBE + 30% Ficol and electrophoresed through a 0.8% agarose gel in 0.25 × TBE at 70 V for 29 hr. at 4°C.

    Staining:

    Article Title: Helicobacter winghamensis sp. nov., a Novel Helicobacter sp. Isolated from Patients with Gastroenteritis
    Article Snippet: .. The resulting amplicon was then digested with endonucleases Dde I and Bsr I (New England Biolabs, Mississauga, Ontario, Canada), and the resulting RFLP patterns were visualized after electrophoresis by ethidium bromide staining. .. For 16S rRNA gene sequencing, chromosomal DNA was first extracted from the isolates using either DNAzol (Molecular Research Center, Inc., Cincinnati, Ohio) or Integrated Separation Systems automated DNA extractor Autogen 540 according to the manufacturer's specifications (Enprotech, Natick, Mass.).

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    New England Biolabs bsr i
    Distribution of polymorphic HML-2 proviruses in breast cancer cases and controls. A . Comparative schematic representing the in silico -predicted sizes for HML-2 containing fragments following <t>Bsr</t> I digestion and detected by the K-seq probe within the Hg19 genome build. Asterisks at left indicate the confirmed polymorphic proviruses, whose distribution coincides exactly between unblot banding patterns and PCR data. B . CPSII samples were sorted by case/control status (n = 25 each) and Bsr I digested <t>WGA-DNA</t> from each group was separated by gel electrophoresis and probed with the 32 P-radiolabeled K-seq oligonucleotide. HML-2 junction fragments were visualized following exposure to film, and polymorphic insertions inferred by variable banding patterns among samples. C . Results from PCR analysis of known polymorphic proviruses for direct comparison of described polymorphic elements, where ‘+’ indicates the confirmed presence of the tested provirus. Novel polymorphic fragments whose identity could not be inferred by comparison to PCR analysis or in silico predictions, have been indicated with arrows at right. Asterisks (at right) are used to indicate the observed fragment sizes of polymorphic elements detected in ≤5% individuals screened here.
    Bsr I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 98/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Distribution of polymorphic HML-2 proviruses in breast cancer cases and controls. A . Comparative schematic representing the in silico -predicted sizes for HML-2 containing fragments following Bsr I digestion and detected by the K-seq probe within the Hg19 genome build. Asterisks at left indicate the confirmed polymorphic proviruses, whose distribution coincides exactly between unblot banding patterns and PCR data. B . CPSII samples were sorted by case/control status (n = 25 each) and Bsr I digested WGA-DNA from each group was separated by gel electrophoresis and probed with the 32 P-radiolabeled K-seq oligonucleotide. HML-2 junction fragments were visualized following exposure to film, and polymorphic insertions inferred by variable banding patterns among samples. C . Results from PCR analysis of known polymorphic proviruses for direct comparison of described polymorphic elements, where ‘+’ indicates the confirmed presence of the tested provirus. Novel polymorphic fragments whose identity could not be inferred by comparison to PCR analysis or in silico predictions, have been indicated with arrows at right. Asterisks (at right) are used to indicate the observed fragment sizes of polymorphic elements detected in ≤5% individuals screened here.

    Journal: Retrovirology

    Article Title: The distribution of insertionally polymorphic endogenous retroviruses in breast cancer patients and cancer-free controls

    doi: 10.1186/s12977-014-0062-3

    Figure Lengend Snippet: Distribution of polymorphic HML-2 proviruses in breast cancer cases and controls. A . Comparative schematic representing the in silico -predicted sizes for HML-2 containing fragments following Bsr I digestion and detected by the K-seq probe within the Hg19 genome build. Asterisks at left indicate the confirmed polymorphic proviruses, whose distribution coincides exactly between unblot banding patterns and PCR data. B . CPSII samples were sorted by case/control status (n = 25 each) and Bsr I digested WGA-DNA from each group was separated by gel electrophoresis and probed with the 32 P-radiolabeled K-seq oligonucleotide. HML-2 junction fragments were visualized following exposure to film, and polymorphic insertions inferred by variable banding patterns among samples. C . Results from PCR analysis of known polymorphic proviruses for direct comparison of described polymorphic elements, where ‘+’ indicates the confirmed presence of the tested provirus. Novel polymorphic fragments whose identity could not be inferred by comparison to PCR analysis or in silico predictions, have been indicated with arrows at right. Asterisks (at right) are used to indicate the observed fragment sizes of polymorphic elements detected in ≤5% individuals screened here.

    Article Snippet: For each sample, 15 μg of WGA DNA was digested with Bsr I (New England Biolabs) in a 100 μL volume and the digested products extracted and precipitated as described above.

    Techniques: In Silico, Polymerase Chain Reaction, Whole Genome Amplification, Nucleic Acid Electrophoresis

    16S rRNA PCR-RFLP patterns for intestinal helicobacters generated using restriction enzyme Bsr I. Lane 1, H. bizzozeronii ; lane 2, H. canadensis ; lane 3, H. canis ; lane 4, H. cinaedi ; lane 5, H. fennelliae ; lane 6, H. pullorum ; lane 7, Helicobacter sp. flexispira taxon 8; lane 8, H. winghamensis ; lane M, 100-bp molecular weight marker (New England Biolabs)

    Journal: Journal of Clinical Microbiology

    Article Title: Helicobacter winghamensis sp. nov., a Novel Helicobacter sp. Isolated from Patients with Gastroenteritis

    doi: 10.1128/JCM.39.7.2412-2417.2001

    Figure Lengend Snippet: 16S rRNA PCR-RFLP patterns for intestinal helicobacters generated using restriction enzyme Bsr I. Lane 1, H. bizzozeronii ; lane 2, H. canadensis ; lane 3, H. canis ; lane 4, H. cinaedi ; lane 5, H. fennelliae ; lane 6, H. pullorum ; lane 7, Helicobacter sp. flexispira taxon 8; lane 8, H. winghamensis ; lane M, 100-bp molecular weight marker (New England Biolabs)

    Article Snippet: The resulting amplicon was then digested with endonucleases Dde I and Bsr I (New England Biolabs, Mississauga, Ontario, Canada), and the resulting RFLP patterns were visualized after electrophoresis by ethidium bromide staining.

    Techniques: Polymerase Chain Reaction, Generated, Molecular Weight, Marker

    Genotyping of -2383 C/T in FOXP3 gene using polymerase chain reaction-restriction fragment length polymorphism technique with Bsr I ( Bse NI) enzyme, followed by the band detection on gel red-stained 3% agarose gel after electrophoresis. 1: pUC19 marker, 2: CC in females and C in males (184, 127, and77 bp), 3: CT (just in females) (261, 184, 127, and 77 bp), 4: TT in females and T in males (261 and 127 bp)

    Journal: Iranian Journal of Basic Medical Sciences

    Article Title: Investigation of FOXP3 genetic variations at positions -2383 C/T and IVS9+459 T/C in southern Iranian patients with lung carcinoma

    doi:

    Figure Lengend Snippet: Genotyping of -2383 C/T in FOXP3 gene using polymerase chain reaction-restriction fragment length polymorphism technique with Bsr I ( Bse NI) enzyme, followed by the band detection on gel red-stained 3% agarose gel after electrophoresis. 1: pUC19 marker, 2: CC in females and C in males (184, 127, and77 bp), 3: CT (just in females) (261, 184, 127, and 77 bp), 4: TT in females and T in males (261 and 127 bp)

    Article Snippet: The products underwent digestion, for 16 hr at 65 °C, with Bsr I (Bse NI) restriction enzyme (NEB, England).

    Techniques: Polymerase Chain Reaction, Staining, Agarose Gel Electrophoresis, Electrophoresis, Marker

    RFLP/MSSCP genotyping of the two nucleotide substitutions and two deletions in promoter of the bovine MEF2 C gene. a RFLP genotyping of the g. - 1606C > T with Bsr I nuclease; b MSSCP genotyping of the DelG at position - 1336_ - 1335 ; C MSSCP genotyping of the C/T transition at position - 818 ; D. MSSCP genotyping of the DelA at position - 613_ - 612

    Journal: Molecular Biology Reports

    Article Title: Nucleotide sequence and variations of the bovine myocyte enhancer factor 2C (MEF2C) gene promoter in Bos Taurus cattle

    doi: 10.1007/s11033-010-0226-8

    Figure Lengend Snippet: RFLP/MSSCP genotyping of the two nucleotide substitutions and two deletions in promoter of the bovine MEF2 C gene. a RFLP genotyping of the g. - 1606C > T with Bsr I nuclease; b MSSCP genotyping of the DelG at position - 1336_ - 1335 ; C MSSCP genotyping of the C/T transition at position - 818 ; D. MSSCP genotyping of the DelA at position - 613_ - 612

    Article Snippet: The 382-bp PCR product including P1 fragment was digested for 3 h with 5 U of Bsr I (New England Biolabs) restriction endonucleases.

    Techniques: