bsri  (New England Biolabs)


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  • 95
    Name:
    BsrI
    Description:
    BsrI 5 000 units
    Catalog Number:
    R0527L
    Price:
    269
    Category:
    Restriction Enzymes
    Size:
    5 000 units
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    Structured Review

    New England Biolabs bsri
    BsrI
    BsrI 5 000 units
    https://www.bioz.com/result/bsri/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bsri - by Bioz Stars, 2021-06
    95/100 stars

    Images

    1) Product Images from "Sclera-related gene polymorphisms in high myopia"

    Article Title: Sclera-related gene polymorphisms in high myopia

    Journal: Molecular Vision

    doi:

    The PCR product of bFGF ( rs308395 ) polymorphism. The PCR product of βFGF2 - rs308395 polymorphism was digested with BsrI. The “ C ” allele was 241 bp and the “ G ” allele was 163 bp + 78 bp.
    Figure Legend Snippet: The PCR product of bFGF ( rs308395 ) polymorphism. The PCR product of βFGF2 - rs308395 polymorphism was digested with BsrI. The “ C ” allele was 241 bp and the “ G ” allele was 163 bp + 78 bp.

    Techniques Used: Polymerase Chain Reaction

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Rapid Identification of Campylobacter, Arcobacter, and Helicobacter Isolates by PCR-Restriction Fragment Length Polymorphism Analysis of the 16S rRNA Gene
    Article Snippet: The samples were subjected to an initial denaturation for 2 min at 95°C, followed by 30 amplification cycles, each consisting of 94°C for 30 s, 52°C for 30 s, and 72°C for 90 s. A final primer extension at 72°C for 10 min was included. .. For restriction endonuclease digestion a 20-μl reaction mixture which included 10 μl of the PCR amplicon with 10 U of the restriction endonuclease Dde I (Boehringer-Mannheim, Indianapolis, Ind.), Taq I (Boehringer-Mannheim), or Bsr I (New England Biolabs, Inc., Beverly, Mass.) was employed, following conditions recommended by the respective manufacturers. .. Ten microliters of each digest was analyzed electrophoretically at 5 V/cm for 2 h with a 3% agarose gel in 0.5× Tris-borate-EDTA (ICN Biomedicals, Aurora, Ohio).

    Article Title: Discrimination of SHV ?-Lactamase Genes by Restriction Site Insertion-PCR
    Article Snippet: PCR amplification was carried out using predenaturation at 95°C for 3 min; followed by 30 cycles consisting of denaturation at 95°C for 30 s, annealing at 60°C for 30 s, and elongation at 72°C for 30 s; and with a final elongation at 72°C for 5 min. .. The PCR products were digested with restriction endonucleases according to the manufacturer's instructions ( Bsr I, Nru I, and Ssp I were purchased from New England Biolabs, Pst I and Hin fI were supplied from Promega and GibcoBRL, respectively). .. After digestion, the products were analysed by gel electrophoresis using 3% low-melting-point agarose (Metaphore; FMC Bio-Products, Flowgen, Staffordshire, United Kingdom).

    Article Title: Sclera-related gene polymorphisms in high myopia
    Article Snippet: .. The PCR product of bFGF ( rs308395 ) polymorphism was digested with BsrI (New England Biolabs). ..

    Article Title: Nucleotide sequence and variations of the bovine myocyte enhancer factor 2C (MEF2C) gene promoter in Bos Taurus cattle
    Article Snippet: .. The 382-bp PCR product including P1 fragment was digested for 3 h with 5 U of Bsr I (New England Biolabs) restriction endonucleases. .. The restriction products were separated by electrophoresis in 3% agarose gel (Sigma-Aldrich, Munich, Germany) with ethidium bromide in 1× TBE buffer.

    Amplification:

    Article Title: Rapid Identification of Campylobacter, Arcobacter, and Helicobacter Isolates by PCR-Restriction Fragment Length Polymorphism Analysis of the 16S rRNA Gene
    Article Snippet: The samples were subjected to an initial denaturation for 2 min at 95°C, followed by 30 amplification cycles, each consisting of 94°C for 30 s, 52°C for 30 s, and 72°C for 90 s. A final primer extension at 72°C for 10 min was included. .. For restriction endonuclease digestion a 20-μl reaction mixture which included 10 μl of the PCR amplicon with 10 U of the restriction endonuclease Dde I (Boehringer-Mannheim, Indianapolis, Ind.), Taq I (Boehringer-Mannheim), or Bsr I (New England Biolabs, Inc., Beverly, Mass.) was employed, following conditions recommended by the respective manufacturers. .. Ten microliters of each digest was analyzed electrophoretically at 5 V/cm for 2 h with a 3% agarose gel in 0.5× Tris-borate-EDTA (ICN Biomedicals, Aurora, Ohio).

    Article Title: Helicobacter winghamensis sp. nov., a Novel Helicobacter sp. Isolated from Patients with Gastroenteritis
    Article Snippet: In brief, chromosomal DNA was isolated from the organisms and subjected to a PCR procedure that amplified a 1-kb portion of the 16S rRNA gene. .. The resulting amplicon was then digested with endonucleases Dde I and Bsr I (New England Biolabs, Mississauga, Ontario, Canada), and the resulting RFLP patterns were visualized after electrophoresis by ethidium bromide staining. .. For 16S rRNA gene sequencing, chromosomal DNA was first extracted from the isolates using either DNAzol (Molecular Research Center, Inc., Cincinnati, Ohio) or Integrated Separation Systems automated DNA extractor Autogen 540 according to the manufacturer's specifications (Enprotech, Natick, Mass.).

    Whole Genome Amplification:

    Article Title: The distribution of insertionally polymorphic endogenous retroviruses in breast cancer patients and cancer-free controls
    Article Snippet: Unblotting Unblotting, or hybridization in semi-dried agarose [ , ], was carried out to visualize polymorphic HERV-K proviruses within DNA samples. .. For each sample, 15 μg of WGA DNA was digested with Bsr I (New England Biolabs) in a 100 μL volume and the digested products extracted and precipitated as described above. .. Products were resuspended in 20uL 0.25 x TBE + 30% Ficol and electrophoresed through a 0.8% agarose gel in 0.25 × TBE at 70 V for 29 hr. at 4°C.

    Electrophoresis:

    Article Title: Helicobacter winghamensis sp. nov., a Novel Helicobacter sp. Isolated from Patients with Gastroenteritis
    Article Snippet: In brief, chromosomal DNA was isolated from the organisms and subjected to a PCR procedure that amplified a 1-kb portion of the 16S rRNA gene. .. The resulting amplicon was then digested with endonucleases Dde I and Bsr I (New England Biolabs, Mississauga, Ontario, Canada), and the resulting RFLP patterns were visualized after electrophoresis by ethidium bromide staining. .. For 16S rRNA gene sequencing, chromosomal DNA was first extracted from the isolates using either DNAzol (Molecular Research Center, Inc., Cincinnati, Ohio) or Integrated Separation Systems automated DNA extractor Autogen 540 according to the manufacturer's specifications (Enprotech, Natick, Mass.).

    Staining:

    Article Title: Helicobacter winghamensis sp. nov., a Novel Helicobacter sp. Isolated from Patients with Gastroenteritis
    Article Snippet: In brief, chromosomal DNA was isolated from the organisms and subjected to a PCR procedure that amplified a 1-kb portion of the 16S rRNA gene. .. The resulting amplicon was then digested with endonucleases Dde I and Bsr I (New England Biolabs, Mississauga, Ontario, Canada), and the resulting RFLP patterns were visualized after electrophoresis by ethidium bromide staining. .. For 16S rRNA gene sequencing, chromosomal DNA was first extracted from the isolates using either DNAzol (Molecular Research Center, Inc., Cincinnati, Ohio) or Integrated Separation Systems automated DNA extractor Autogen 540 according to the manufacturer's specifications (Enprotech, Natick, Mass.).

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    New England Biolabs bsr i
    Distribution of polymorphic HML-2 proviruses in breast cancer cases and controls. A . Comparative schematic representing the in silico -predicted sizes for HML-2 containing fragments following <t>Bsr</t> I digestion and detected by the K-seq probe within the Hg19 genome build. Asterisks at left indicate the confirmed polymorphic proviruses, whose distribution coincides exactly between unblot banding patterns and PCR data. B . CPSII samples were sorted by case/control status (n = 25 each) and Bsr I digested <t>WGA-DNA</t> from each group was separated by gel electrophoresis and probed with the 32 P-radiolabeled K-seq oligonucleotide. HML-2 junction fragments were visualized following exposure to film, and polymorphic insertions inferred by variable banding patterns among samples. C . Results from PCR analysis of known polymorphic proviruses for direct comparison of described polymorphic elements, where ‘+’ indicates the confirmed presence of the tested provirus. Novel polymorphic fragments whose identity could not be inferred by comparison to PCR analysis or in silico predictions, have been indicated with arrows at right. Asterisks (at right) are used to indicate the observed fragment sizes of polymorphic elements detected in ≤5% individuals screened here.
    Bsr I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bsr i/product/New England Biolabs
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    bsr i - by Bioz Stars, 2021-06
    95/100 stars
      Buy from Supplier

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    Distribution of polymorphic HML-2 proviruses in breast cancer cases and controls. A . Comparative schematic representing the in silico -predicted sizes for HML-2 containing fragments following Bsr I digestion and detected by the K-seq probe within the Hg19 genome build. Asterisks at left indicate the confirmed polymorphic proviruses, whose distribution coincides exactly between unblot banding patterns and PCR data. B . CPSII samples were sorted by case/control status (n = 25 each) and Bsr I digested WGA-DNA from each group was separated by gel electrophoresis and probed with the 32 P-radiolabeled K-seq oligonucleotide. HML-2 junction fragments were visualized following exposure to film, and polymorphic insertions inferred by variable banding patterns among samples. C . Results from PCR analysis of known polymorphic proviruses for direct comparison of described polymorphic elements, where ‘+’ indicates the confirmed presence of the tested provirus. Novel polymorphic fragments whose identity could not be inferred by comparison to PCR analysis or in silico predictions, have been indicated with arrows at right. Asterisks (at right) are used to indicate the observed fragment sizes of polymorphic elements detected in ≤5% individuals screened here.

    Journal: Retrovirology

    Article Title: The distribution of insertionally polymorphic endogenous retroviruses in breast cancer patients and cancer-free controls

    doi: 10.1186/s12977-014-0062-3

    Figure Lengend Snippet: Distribution of polymorphic HML-2 proviruses in breast cancer cases and controls. A . Comparative schematic representing the in silico -predicted sizes for HML-2 containing fragments following Bsr I digestion and detected by the K-seq probe within the Hg19 genome build. Asterisks at left indicate the confirmed polymorphic proviruses, whose distribution coincides exactly between unblot banding patterns and PCR data. B . CPSII samples were sorted by case/control status (n = 25 each) and Bsr I digested WGA-DNA from each group was separated by gel electrophoresis and probed with the 32 P-radiolabeled K-seq oligonucleotide. HML-2 junction fragments were visualized following exposure to film, and polymorphic insertions inferred by variable banding patterns among samples. C . Results from PCR analysis of known polymorphic proviruses for direct comparison of described polymorphic elements, where ‘+’ indicates the confirmed presence of the tested provirus. Novel polymorphic fragments whose identity could not be inferred by comparison to PCR analysis or in silico predictions, have been indicated with arrows at right. Asterisks (at right) are used to indicate the observed fragment sizes of polymorphic elements detected in ≤5% individuals screened here.

    Article Snippet: For each sample, 15 μg of WGA DNA was digested with Bsr I (New England Biolabs) in a 100 μL volume and the digested products extracted and precipitated as described above.

    Techniques: In Silico, Polymerase Chain Reaction, Whole Genome Amplification, Nucleic Acid Electrophoresis

    Mutations of bla SHV genes demonstrated by RSI-PCR analysis with various restriction endonucleases. (a) Position 8 with Ssp I digestion; (b) position 179 with Hin fI digestion; (c) position 238 with Bsr I digestion. Lanes A to H, amplimers of bla SHV genes bla SHV-1 (A), bla SHV-2 (B), bla SHV-3 (C), bla SHV-4 (D), bla SHV-5 (E), bla SHV-6 (F), bla SHV-8 (G), and bla SHV-18 (H) after digestion; lane I, amplimer of bla SHV-1 gene before digestion; lane M, 100-bp ladder.

    Journal: Antimicrobial Agents and Chemotherapy

    Article Title: Discrimination of SHV ?-Lactamase Genes by Restriction Site Insertion-PCR

    doi: 10.1128/AAC.45.7.2110-2114.2001

    Figure Lengend Snippet: Mutations of bla SHV genes demonstrated by RSI-PCR analysis with various restriction endonucleases. (a) Position 8 with Ssp I digestion; (b) position 179 with Hin fI digestion; (c) position 238 with Bsr I digestion. Lanes A to H, amplimers of bla SHV genes bla SHV-1 (A), bla SHV-2 (B), bla SHV-3 (C), bla SHV-4 (D), bla SHV-5 (E), bla SHV-6 (F), bla SHV-8 (G), and bla SHV-18 (H) after digestion; lane I, amplimer of bla SHV-1 gene before digestion; lane M, 100-bp ladder.

    Article Snippet: The PCR products were digested with restriction endonucleases according to the manufacturer's instructions ( Bsr I, Nru I, and Ssp I were purchased from New England Biolabs, Pst I and Hin fI were supplied from Promega and GibcoBRL, respectively).

    Techniques: Polymerase Chain Reaction

    Genotyping of -2383 C/T in FOXP3 gene using polymerase chain reaction-restriction fragment length polymorphism technique with Bsr I ( Bse NI) enzyme, followed by the band detection on gel red-stained 3% agarose gel after electrophoresis. 1: pUC19 marker, 2: CC in females and C in males (184, 127, and77 bp), 3: CT (just in females) (261, 184, 127, and 77 bp), 4: TT in females and T in males (261 and 127 bp)

    Journal: Iranian Journal of Basic Medical Sciences

    Article Title: Investigation of FOXP3 genetic variations at positions -2383 C/T and IVS9+459 T/C in southern Iranian patients with lung carcinoma

    doi:

    Figure Lengend Snippet: Genotyping of -2383 C/T in FOXP3 gene using polymerase chain reaction-restriction fragment length polymorphism technique with Bsr I ( Bse NI) enzyme, followed by the band detection on gel red-stained 3% agarose gel after electrophoresis. 1: pUC19 marker, 2: CC in females and C in males (184, 127, and77 bp), 3: CT (just in females) (261, 184, 127, and 77 bp), 4: TT in females and T in males (261 and 127 bp)

    Article Snippet: The products underwent digestion, for 16 hr at 65 °C, with Bsr I (Bse NI) restriction enzyme (NEB, England).

    Techniques: Polymerase Chain Reaction, Staining, Agarose Gel Electrophoresis, Electrophoresis, Marker

    a Electrophoretogram of DNA fragments for Ser680Asn polymorphism after digestion with Bsr I. Homozygote A/A was shown by the band of 520 bp. Homozygote G/G was shown by the bands of 413 bp and 107 bp. Heterozygote A/G was shown by the bands of 520 bp, 413 bp and 107 bp. b DNA sequencing of the Ser680Asn polymorphisms (AA, AG and GG) as indicated by arrows . The lane marked M is the marker of DNA. The lane marked P is the DNA of PCOS without enzyme digestion

    Journal: Journal of Assisted Reproduction and Genetics

    Article Title: Association between FSHR polymorphisms and polycystic ovary syndrome among Chinese women in north China

    doi: 10.1007/s10815-013-0166-z

    Figure Lengend Snippet: a Electrophoretogram of DNA fragments for Ser680Asn polymorphism after digestion with Bsr I. Homozygote A/A was shown by the band of 520 bp. Homozygote G/G was shown by the bands of 413 bp and 107 bp. Heterozygote A/G was shown by the bands of 520 bp, 413 bp and 107 bp. b DNA sequencing of the Ser680Asn polymorphisms (AA, AG and GG) as indicated by arrows . The lane marked M is the marker of DNA. The lane marked P is the DNA of PCOS without enzyme digestion

    Article Snippet: Restriction enzymes Ahd I and Bsr I (all from New England Biolabs, Ipswich, MA, USA) were used for Ala307Thr and Ser680Asn, respectively.

    Techniques: DNA Sequencing, Marker