bsr i  (New England Biolabs)


Bioz Verified Symbol New England Biolabs is a verified supplier
Bioz Manufacturer Symbol New England Biolabs manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Name:
    BsrI
    Description:
    BsrI 5 000 units
    Catalog Number:
    r0527l
    Price:
    269
    Size:
    5 000 units
    Category:
    Restriction Enzymes
    Buy from Supplier


    Structured Review

    New England Biolabs bsr i
    BsrI
    BsrI 5 000 units
    https://www.bioz.com/result/bsr i/product/New England Biolabs
    Average 94 stars, based on 16373 article reviews
    Price from $9.99 to $1999.99
    bsr i - by Bioz Stars, 2020-07
    94/100 stars

    Images

    1) Product Images from "The distribution of insertionally polymorphic endogenous retroviruses in breast cancer patients and cancer-free controls"

    Article Title: The distribution of insertionally polymorphic endogenous retroviruses in breast cancer patients and cancer-free controls

    Journal: Retrovirology

    doi: 10.1186/s12977-014-0062-3

    Distribution of polymorphic HML-2 proviruses in breast cancer cases and controls. A . Comparative schematic representing the in silico -predicted sizes for HML-2 containing fragments following Bsr I digestion and detected by the K-seq probe within the Hg19 genome build. Asterisks at left indicate the confirmed polymorphic proviruses, whose distribution coincides exactly between unblot banding patterns and PCR data. B . CPSII samples were sorted by case/control status (n = 25 each) and Bsr I digested WGA-DNA from each group was separated by gel electrophoresis and probed with the 32 P-radiolabeled K-seq oligonucleotide. HML-2 junction fragments were visualized following exposure to film, and polymorphic insertions inferred by variable banding patterns among samples. C . Results from PCR analysis of known polymorphic proviruses for direct comparison of described polymorphic elements, where ‘+’ indicates the confirmed presence of the tested provirus. Novel polymorphic fragments whose identity could not be inferred by comparison to PCR analysis or in silico predictions, have been indicated with arrows at right. Asterisks (at right) are used to indicate the observed fragment sizes of polymorphic elements detected in ≤5% individuals screened here.
    Figure Legend Snippet: Distribution of polymorphic HML-2 proviruses in breast cancer cases and controls. A . Comparative schematic representing the in silico -predicted sizes for HML-2 containing fragments following Bsr I digestion and detected by the K-seq probe within the Hg19 genome build. Asterisks at left indicate the confirmed polymorphic proviruses, whose distribution coincides exactly between unblot banding patterns and PCR data. B . CPSII samples were sorted by case/control status (n = 25 each) and Bsr I digested WGA-DNA from each group was separated by gel electrophoresis and probed with the 32 P-radiolabeled K-seq oligonucleotide. HML-2 junction fragments were visualized following exposure to film, and polymorphic insertions inferred by variable banding patterns among samples. C . Results from PCR analysis of known polymorphic proviruses for direct comparison of described polymorphic elements, where ‘+’ indicates the confirmed presence of the tested provirus. Novel polymorphic fragments whose identity could not be inferred by comparison to PCR analysis or in silico predictions, have been indicated with arrows at right. Asterisks (at right) are used to indicate the observed fragment sizes of polymorphic elements detected in ≤5% individuals screened here.

    Techniques Used: In Silico, Polymerase Chain Reaction, Whole Genome Amplification, Nucleic Acid Electrophoresis

    2) Product Images from "Helicobacter winghamensis sp. nov., a Novel Helicobacter sp. Isolated from Patients with Gastroenteritis"

    Article Title: Helicobacter winghamensis sp. nov., a Novel Helicobacter sp. Isolated from Patients with Gastroenteritis

    Journal: Journal of Clinical Microbiology

    doi: 10.1128/JCM.39.7.2412-2417.2001

    16S rRNA PCR-RFLP patterns for intestinal helicobacters generated using restriction enzyme Bsr I. Lane 1, H. bizzozeronii ; lane 2, H. canadensis ; lane 3, H. canis ; lane 4, H. cinaedi ; lane 5, H. fennelliae ; lane 6, H. pullorum ; lane 7, Helicobacter sp. flexispira taxon 8; lane 8, H. winghamensis ; lane M, 100-bp molecular weight marker (New England Biolabs)
    Figure Legend Snippet: 16S rRNA PCR-RFLP patterns for intestinal helicobacters generated using restriction enzyme Bsr I. Lane 1, H. bizzozeronii ; lane 2, H. canadensis ; lane 3, H. canis ; lane 4, H. cinaedi ; lane 5, H. fennelliae ; lane 6, H. pullorum ; lane 7, Helicobacter sp. flexispira taxon 8; lane 8, H. winghamensis ; lane M, 100-bp molecular weight marker (New England Biolabs)

    Techniques Used: Polymerase Chain Reaction, Generated, Molecular Weight, Marker

    3) Product Images from "Investigation of FOXP3 genetic variations at positions -2383 C/T and IVS9+459 T/C in southern Iranian patients with lung carcinoma"

    Article Title: Investigation of FOXP3 genetic variations at positions -2383 C/T and IVS9+459 T/C in southern Iranian patients with lung carcinoma

    Journal: Iranian Journal of Basic Medical Sciences

    doi:

    Genotyping of -2383 C/T in FOXP3 gene using polymerase chain reaction-restriction fragment length polymorphism technique with Bsr I ( Bse NI) enzyme, followed by the band detection on gel red-stained 3% agarose gel after electrophoresis. 1: pUC19 marker, 2: CC in females and C in males (184, 127, and77 bp), 3: CT (just in females) (261, 184, 127, and 77 bp), 4: TT in females and T in males (261 and 127 bp)
    Figure Legend Snippet: Genotyping of -2383 C/T in FOXP3 gene using polymerase chain reaction-restriction fragment length polymorphism technique with Bsr I ( Bse NI) enzyme, followed by the band detection on gel red-stained 3% agarose gel after electrophoresis. 1: pUC19 marker, 2: CC in females and C in males (184, 127, and77 bp), 3: CT (just in females) (261, 184, 127, and 77 bp), 4: TT in females and T in males (261 and 127 bp)

    Techniques Used: Polymerase Chain Reaction, Staining, Agarose Gel Electrophoresis, Electrophoresis, Marker

    4) Product Images from "Comparison of the Complete DNA Sequences of the Oka Varicella Vaccine and Its Parental Virus"

    Article Title: Comparison of the Complete DNA Sequences of the Oka Varicella Vaccine and Its Parental Virus

    Journal: Journal of Virology

    doi: 10.1128/JVI.76.22.11447-11459.2002

    RFLP analysis of the PCR products from V-Oka, P-Oka, and 54 wild-type viruses. V-Oka (V) can be distinguished not only from P-Oka (P) but also from all wild-type viruses. The Kawaguchi strain (K) was used to represent the wild-type viruses by using Alu I (a), Bst XI (b), Acc II (c), Sfa NI (d), Sac II (e), Sma I (f), and Bsr I (g).
    Figure Legend Snippet: RFLP analysis of the PCR products from V-Oka, P-Oka, and 54 wild-type viruses. V-Oka (V) can be distinguished not only from P-Oka (P) but also from all wild-type viruses. The Kawaguchi strain (K) was used to represent the wild-type viruses by using Alu I (a), Bst XI (b), Acc II (c), Sfa NI (d), Sac II (e), Sma I (f), and Bsr I (g).

    Techniques Used: Polymerase Chain Reaction

    5) Product Images from "Nucleotide sequence and variations of the bovine myocyte enhancer factor 2C (MEF2C) gene promoter in Bos Taurus cattle"

    Article Title: Nucleotide sequence and variations of the bovine myocyte enhancer factor 2C (MEF2C) gene promoter in Bos Taurus cattle

    Journal: Molecular Biology Reports

    doi: 10.1007/s11033-010-0226-8

    RFLP/MSSCP genotyping of the two nucleotide substitutions and two deletions in promoter of the bovine MEF2 C gene. a RFLP genotyping of the g. - 1606C > T with Bsr I nuclease; b MSSCP genotyping of the DelG at position - 1336_ - 1335 ; C MSSCP genotyping of the C/T transition at position - 818 ; D. MSSCP genotyping of the DelA at position - 613_ - 612
    Figure Legend Snippet: RFLP/MSSCP genotyping of the two nucleotide substitutions and two deletions in promoter of the bovine MEF2 C gene. a RFLP genotyping of the g. - 1606C > T with Bsr I nuclease; b MSSCP genotyping of the DelG at position - 1336_ - 1335 ; C MSSCP genotyping of the C/T transition at position - 818 ; D. MSSCP genotyping of the DelA at position - 613_ - 612

    Techniques Used:

    6) Product Images from "Sclera-related gene polymorphisms in high myopia"

    Article Title: Sclera-related gene polymorphisms in high myopia

    Journal: Molecular Vision

    doi:

    The PCR product of bFGF ( rs308395 ) polymorphism. The PCR product of βFGF2 - rs308395 polymorphism was digested with BsrI. The “ C ” allele was 241 bp and the “ G ” allele was 163 bp + 78 bp.
    Figure Legend Snippet: The PCR product of bFGF ( rs308395 ) polymorphism. The PCR product of βFGF2 - rs308395 polymorphism was digested with BsrI. The “ C ” allele was 241 bp and the “ G ” allele was 163 bp + 78 bp.

    Techniques Used: Polymerase Chain Reaction

    7) Product Images from "Rapid Identification of Campylobacter, Arcobacter, and Helicobacter Isolates by PCR-Restriction Fragment Length Polymorphism Analysis of the 16S rRNA Gene"

    Article Title: Rapid Identification of Campylobacter, Arcobacter, and Helicobacter Isolates by PCR-Restriction Fragment Length Polymorphism Analysis of the 16S rRNA Gene

    Journal: Journal of Clinical Microbiology

    doi:

    (A) 16S PCR-RFLP patterns observed with Dde I. (B) 16S PCR-RFLP patterns observed with Taq I and Bsr I. Letter above each lane denotes pattern obtained. Lane M, 100-bp ladder (New England Biolabs). The numbers are molecular sizes in kilobases.
    Figure Legend Snippet: (A) 16S PCR-RFLP patterns observed with Dde I. (B) 16S PCR-RFLP patterns observed with Taq I and Bsr I. Letter above each lane denotes pattern obtained. Lane M, 100-bp ladder (New England Biolabs). The numbers are molecular sizes in kilobases.

    Techniques Used: Polymerase Chain Reaction

    8) Product Images from "Discrimination of SHV ?-Lactamase Genes by Restriction Site Insertion-PCR"

    Article Title: Discrimination of SHV ?-Lactamase Genes by Restriction Site Insertion-PCR

    Journal: Antimicrobial Agents and Chemotherapy

    doi: 10.1128/AAC.45.7.2110-2114.2001

    Mutations of bla SHV genes demonstrated by RSI-PCR analysis with various restriction endonucleases. (a) Position 8 with Ssp I digestion; (b) position 179 with Hin fI digestion; (c) position 238 with Bsr I digestion. Lanes A to H, amplimers of bla SHV genes bla SHV-1 (A), bla SHV-2 (B), bla SHV-3 (C), bla SHV-4 (D), bla SHV-5 (E), bla SHV-6 (F), bla SHV-8 (G), and bla SHV-18 (H) after digestion; lane I, amplimer of bla SHV-1 gene before digestion; lane M, 100-bp ladder.
    Figure Legend Snippet: Mutations of bla SHV genes demonstrated by RSI-PCR analysis with various restriction endonucleases. (a) Position 8 with Ssp I digestion; (b) position 179 with Hin fI digestion; (c) position 238 with Bsr I digestion. Lanes A to H, amplimers of bla SHV genes bla SHV-1 (A), bla SHV-2 (B), bla SHV-3 (C), bla SHV-4 (D), bla SHV-5 (E), bla SHV-6 (F), bla SHV-8 (G), and bla SHV-18 (H) after digestion; lane I, amplimer of bla SHV-1 gene before digestion; lane M, 100-bp ladder.

    Techniques Used: Polymerase Chain Reaction

    9) Product Images from "Association between FSHR polymorphisms and polycystic ovary syndrome among Chinese women in north China"

    Article Title: Association between FSHR polymorphisms and polycystic ovary syndrome among Chinese women in north China

    Journal: Journal of Assisted Reproduction and Genetics

    doi: 10.1007/s10815-013-0166-z

    a Electrophoretogram of DNA fragments for Ser680Asn polymorphism after digestion with Bsr I. Homozygote A/A was shown by the band of 520 bp. Homozygote G/G was shown by the bands of 413 bp and 107 bp. Heterozygote A/G was shown by the bands of 520 bp, 413 bp and 107 bp. b DNA sequencing of the Ser680Asn polymorphisms (AA, AG and GG) as indicated by arrows . The lane marked M is the marker of DNA. The lane marked P is the DNA of PCOS without enzyme digestion
    Figure Legend Snippet: a Electrophoretogram of DNA fragments for Ser680Asn polymorphism after digestion with Bsr I. Homozygote A/A was shown by the band of 520 bp. Homozygote G/G was shown by the bands of 413 bp and 107 bp. Heterozygote A/G was shown by the bands of 520 bp, 413 bp and 107 bp. b DNA sequencing of the Ser680Asn polymorphisms (AA, AG and GG) as indicated by arrows . The lane marked M is the marker of DNA. The lane marked P is the DNA of PCOS without enzyme digestion

    Techniques Used: DNA Sequencing, Marker

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Sclera-related gene polymorphisms in high myopia
    Article Snippet: .. The PCR product of bFGF ( rs308395 ) polymorphism was digested with BsrI (New England Biolabs). ..

    Article Title: Rapid Identification of Campylobacter, Arcobacter, and Helicobacter Isolates by PCR-Restriction Fragment Length Polymorphism Analysis of the 16S rRNA Gene
    Article Snippet: .. For restriction endonuclease digestion a 20-μl reaction mixture which included 10 μl of the PCR amplicon with 10 U of the restriction endonuclease Dde I (Boehringer-Mannheim, Indianapolis, Ind.), Taq I (Boehringer-Mannheim), or Bsr I (New England Biolabs, Inc., Beverly, Mass.) was employed, following conditions recommended by the respective manufacturers. .. Ten microliters of each digest was analyzed electrophoretically at 5 V/cm for 2 h with a 3% agarose gel in 0.5× Tris-borate-EDTA (ICN Biomedicals, Aurora, Ohio).

    Article Title: Nucleotide sequence and variations of the bovine myocyte enhancer factor 2C (MEF2C) gene promoter in Bos Taurus cattle
    Article Snippet: .. The 382-bp PCR product including P1 fragment was digested for 3 h with 5 U of Bsr I (New England Biolabs) restriction endonucleases. .. The restriction products were separated by electrophoresis in 3% agarose gel (Sigma-Aldrich, Munich, Germany) with ethidium bromide in 1× TBE buffer.

    Article Title: Discrimination of SHV ?-Lactamase Genes by Restriction Site Insertion-PCR
    Article Snippet: .. The PCR products were digested with restriction endonucleases according to the manufacturer's instructions ( Bsr I, Nru I, and Ssp I were purchased from New England Biolabs, Pst I and Hin fI were supplied from Promega and GibcoBRL, respectively). .. After digestion, the products were analysed by gel electrophoresis using 3% low-melting-point agarose (Metaphore; FMC Bio-Products, Flowgen, Staffordshire, United Kingdom).

    Electrophoresis:

    Article Title: Helicobacter winghamensis sp. nov., a Novel Helicobacter sp. Isolated from Patients with Gastroenteritis
    Article Snippet: .. The resulting amplicon was then digested with endonucleases Dde I and Bsr I (New England Biolabs, Mississauga, Ontario, Canada), and the resulting RFLP patterns were visualized after electrophoresis by ethidium bromide staining. .. For 16S rRNA gene sequencing, chromosomal DNA was first extracted from the isolates using either DNAzol (Molecular Research Center, Inc., Cincinnati, Ohio) or Integrated Separation Systems automated DNA extractor Autogen 540 according to the manufacturer's specifications (Enprotech, Natick, Mass.).

    Amplification:

    Article Title: Helicobacter winghamensis sp. nov., a Novel Helicobacter sp. Isolated from Patients with Gastroenteritis
    Article Snippet: .. The resulting amplicon was then digested with endonucleases Dde I and Bsr I (New England Biolabs, Mississauga, Ontario, Canada), and the resulting RFLP patterns were visualized after electrophoresis by ethidium bromide staining. .. For 16S rRNA gene sequencing, chromosomal DNA was first extracted from the isolates using either DNAzol (Molecular Research Center, Inc., Cincinnati, Ohio) or Integrated Separation Systems automated DNA extractor Autogen 540 according to the manufacturer's specifications (Enprotech, Natick, Mass.).

    Article Title: Rapid Identification of Campylobacter, Arcobacter, and Helicobacter Isolates by PCR-Restriction Fragment Length Polymorphism Analysis of the 16S rRNA Gene
    Article Snippet: .. For restriction endonuclease digestion a 20-μl reaction mixture which included 10 μl of the PCR amplicon with 10 U of the restriction endonuclease Dde I (Boehringer-Mannheim, Indianapolis, Ind.), Taq I (Boehringer-Mannheim), or Bsr I (New England Biolabs, Inc., Beverly, Mass.) was employed, following conditions recommended by the respective manufacturers. .. Ten microliters of each digest was analyzed electrophoretically at 5 V/cm for 2 h with a 3% agarose gel in 0.5× Tris-borate-EDTA (ICN Biomedicals, Aurora, Ohio).

    Article Title: Comparison of the Complete DNA Sequences of the Oka Varicella Vaccine and Its Parental Virus
    Article Snippet: .. Portions (3 μl) of the amplified products with three primer pairs—01-N12 and 01-R13, 50-N15 and 50-R17, or 60-N11 and 60-R12—were digested with 4 U of the restriction endonucleases Alu I (Takara Shuzo), Bst XI (Takara Shuzo), or Bsr I (New England Biolabs), respectively. .. The same volumes of the products obtained by using primer pair 60-N06 and 60-R06 and primer pair 60-N26 and 60-R28 were digested independently with 4 U of the enzymes Sfa NI (New England Biolabs)- Acc II (Takara Shuzo) and Sac II (New England Biolabs)- Sma I (New England Biolabs), respectively.

    Whole Genome Amplification:

    Article Title: The distribution of insertionally polymorphic endogenous retroviruses in breast cancer patients and cancer-free controls
    Article Snippet: .. For each sample, 15 μg of WGA DNA was digested with Bsr I (New England Biolabs) in a 100 μL volume and the digested products extracted and precipitated as described above. .. Products were resuspended in 20uL 0.25 x TBE + 30% Ficol and electrophoresed through a 0.8% agarose gel in 0.25 × TBE at 70 V for 29 hr. at 4°C.

    Staining:

    Article Title: Helicobacter winghamensis sp. nov., a Novel Helicobacter sp. Isolated from Patients with Gastroenteritis
    Article Snippet: .. The resulting amplicon was then digested with endonucleases Dde I and Bsr I (New England Biolabs, Mississauga, Ontario, Canada), and the resulting RFLP patterns were visualized after electrophoresis by ethidium bromide staining. .. For 16S rRNA gene sequencing, chromosomal DNA was first extracted from the isolates using either DNAzol (Molecular Research Center, Inc., Cincinnati, Ohio) or Integrated Separation Systems automated DNA extractor Autogen 540 according to the manufacturer's specifications (Enprotech, Natick, Mass.).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    New England Biolabs bsr i
    Distribution of polymorphic HML-2 proviruses in breast cancer cases and controls. A . Comparative schematic representing the in silico -predicted sizes for HML-2 containing fragments following <t>Bsr</t> I digestion and detected by the K-seq probe within the Hg19 genome build. Asterisks at left indicate the confirmed polymorphic proviruses, whose distribution coincides exactly between unblot banding patterns and PCR data. B . CPSII samples were sorted by case/control status (n = 25 each) and Bsr I digested <t>WGA-DNA</t> from each group was separated by gel electrophoresis and probed with the 32 P-radiolabeled K-seq oligonucleotide. HML-2 junction fragments were visualized following exposure to film, and polymorphic insertions inferred by variable banding patterns among samples. C . Results from PCR analysis of known polymorphic proviruses for direct comparison of described polymorphic elements, where ‘+’ indicates the confirmed presence of the tested provirus. Novel polymorphic fragments whose identity could not be inferred by comparison to PCR analysis or in silico predictions, have been indicated with arrows at right. Asterisks (at right) are used to indicate the observed fragment sizes of polymorphic elements detected in ≤5% individuals screened here.
    Bsr I, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 94/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/bsr i/product/New England Biolabs
    Average 94 stars, based on 10 article reviews
    Price from $9.99 to $1999.99
    bsr i - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    Image Search Results


    Distribution of polymorphic HML-2 proviruses in breast cancer cases and controls. A . Comparative schematic representing the in silico -predicted sizes for HML-2 containing fragments following Bsr I digestion and detected by the K-seq probe within the Hg19 genome build. Asterisks at left indicate the confirmed polymorphic proviruses, whose distribution coincides exactly between unblot banding patterns and PCR data. B . CPSII samples were sorted by case/control status (n = 25 each) and Bsr I digested WGA-DNA from each group was separated by gel electrophoresis and probed with the 32 P-radiolabeled K-seq oligonucleotide. HML-2 junction fragments were visualized following exposure to film, and polymorphic insertions inferred by variable banding patterns among samples. C . Results from PCR analysis of known polymorphic proviruses for direct comparison of described polymorphic elements, where ‘+’ indicates the confirmed presence of the tested provirus. Novel polymorphic fragments whose identity could not be inferred by comparison to PCR analysis or in silico predictions, have been indicated with arrows at right. Asterisks (at right) are used to indicate the observed fragment sizes of polymorphic elements detected in ≤5% individuals screened here.

    Journal: Retrovirology

    Article Title: The distribution of insertionally polymorphic endogenous retroviruses in breast cancer patients and cancer-free controls

    doi: 10.1186/s12977-014-0062-3

    Figure Lengend Snippet: Distribution of polymorphic HML-2 proviruses in breast cancer cases and controls. A . Comparative schematic representing the in silico -predicted sizes for HML-2 containing fragments following Bsr I digestion and detected by the K-seq probe within the Hg19 genome build. Asterisks at left indicate the confirmed polymorphic proviruses, whose distribution coincides exactly between unblot banding patterns and PCR data. B . CPSII samples were sorted by case/control status (n = 25 each) and Bsr I digested WGA-DNA from each group was separated by gel electrophoresis and probed with the 32 P-radiolabeled K-seq oligonucleotide. HML-2 junction fragments were visualized following exposure to film, and polymorphic insertions inferred by variable banding patterns among samples. C . Results from PCR analysis of known polymorphic proviruses for direct comparison of described polymorphic elements, where ‘+’ indicates the confirmed presence of the tested provirus. Novel polymorphic fragments whose identity could not be inferred by comparison to PCR analysis or in silico predictions, have been indicated with arrows at right. Asterisks (at right) are used to indicate the observed fragment sizes of polymorphic elements detected in ≤5% individuals screened here.

    Article Snippet: For each sample, 15 μg of WGA DNA was digested with Bsr I (New England Biolabs) in a 100 μL volume and the digested products extracted and precipitated as described above.

    Techniques: In Silico, Polymerase Chain Reaction, Whole Genome Amplification, Nucleic Acid Electrophoresis

    16S rRNA PCR-RFLP patterns for intestinal helicobacters generated using restriction enzyme Bsr I. Lane 1, H. bizzozeronii ; lane 2, H. canadensis ; lane 3, H. canis ; lane 4, H. cinaedi ; lane 5, H. fennelliae ; lane 6, H. pullorum ; lane 7, Helicobacter sp. flexispira taxon 8; lane 8, H. winghamensis ; lane M, 100-bp molecular weight marker (New England Biolabs)

    Journal: Journal of Clinical Microbiology

    Article Title: Helicobacter winghamensis sp. nov., a Novel Helicobacter sp. Isolated from Patients with Gastroenteritis

    doi: 10.1128/JCM.39.7.2412-2417.2001

    Figure Lengend Snippet: 16S rRNA PCR-RFLP patterns for intestinal helicobacters generated using restriction enzyme Bsr I. Lane 1, H. bizzozeronii ; lane 2, H. canadensis ; lane 3, H. canis ; lane 4, H. cinaedi ; lane 5, H. fennelliae ; lane 6, H. pullorum ; lane 7, Helicobacter sp. flexispira taxon 8; lane 8, H. winghamensis ; lane M, 100-bp molecular weight marker (New England Biolabs)

    Article Snippet: The resulting amplicon was then digested with endonucleases Dde I and Bsr I (New England Biolabs, Mississauga, Ontario, Canada), and the resulting RFLP patterns were visualized after electrophoresis by ethidium bromide staining.

    Techniques: Polymerase Chain Reaction, Generated, Molecular Weight, Marker

    Genotyping of -2383 C/T in FOXP3 gene using polymerase chain reaction-restriction fragment length polymorphism technique with Bsr I ( Bse NI) enzyme, followed by the band detection on gel red-stained 3% agarose gel after electrophoresis. 1: pUC19 marker, 2: CC in females and C in males (184, 127, and77 bp), 3: CT (just in females) (261, 184, 127, and 77 bp), 4: TT in females and T in males (261 and 127 bp)

    Journal: Iranian Journal of Basic Medical Sciences

    Article Title: Investigation of FOXP3 genetic variations at positions -2383 C/T and IVS9+459 T/C in southern Iranian patients with lung carcinoma

    doi:

    Figure Lengend Snippet: Genotyping of -2383 C/T in FOXP3 gene using polymerase chain reaction-restriction fragment length polymorphism technique with Bsr I ( Bse NI) enzyme, followed by the band detection on gel red-stained 3% agarose gel after electrophoresis. 1: pUC19 marker, 2: CC in females and C in males (184, 127, and77 bp), 3: CT (just in females) (261, 184, 127, and 77 bp), 4: TT in females and T in males (261 and 127 bp)

    Article Snippet: The products underwent digestion, for 16 hr at 65 °C, with Bsr I (Bse NI) restriction enzyme (NEB, England).

    Techniques: Polymerase Chain Reaction, Staining, Agarose Gel Electrophoresis, Electrophoresis, Marker

    RFLP analysis of the PCR products from V-Oka, P-Oka, and 54 wild-type viruses. V-Oka (V) can be distinguished not only from P-Oka (P) but also from all wild-type viruses. The Kawaguchi strain (K) was used to represent the wild-type viruses by using Alu I (a), Bst XI (b), Acc II (c), Sfa NI (d), Sac II (e), Sma I (f), and Bsr I (g).

    Journal: Journal of Virology

    Article Title: Comparison of the Complete DNA Sequences of the Oka Varicella Vaccine and Its Parental Virus

    doi: 10.1128/JVI.76.22.11447-11459.2002

    Figure Lengend Snippet: RFLP analysis of the PCR products from V-Oka, P-Oka, and 54 wild-type viruses. V-Oka (V) can be distinguished not only from P-Oka (P) but also from all wild-type viruses. The Kawaguchi strain (K) was used to represent the wild-type viruses by using Alu I (a), Bst XI (b), Acc II (c), Sfa NI (d), Sac II (e), Sma I (f), and Bsr I (g).

    Article Snippet: Portions (3 μl) of the amplified products with three primer pairs—01-N12 and 01-R13, 50-N15 and 50-R17, or 60-N11 and 60-R12—were digested with 4 U of the restriction endonucleases Alu I (Takara Shuzo), Bst XI (Takara Shuzo), or Bsr I (New England Biolabs), respectively.

    Techniques: Polymerase Chain Reaction