asei  (New England Biolabs)


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    New England Biolabs asei
    3C Analysis of the interaction between the mbr and the BCL2 promoter region in vivo . The scheme of BCL2 gene with the positions of restriction sites and positions of primers was indicated ( A ). The 3C experiments were performed as described in the ‘Materials and Methods’ section. Briefly, formaldehyde was used to crosslink protein–DNA interactions in intact nuclei. The crosslinked chromatin was then digested by <t>AseI</t> ( B ) or HindIII ( C ), followed by ligation. Physical interaction between mbr and <t>SBS1</t> or SBS2 was then determined by specific PCRs that detected hybrid fragments containing either mbr/SBS1 sequences or mbr/SBS2 sequences. 3C data demonstrated that the mbr specifically interacted with SBS1 (B, up panel), but not with SBS2 (C, up panel). PCR products from AseI (B) or HindIII (C) digested crosslinked chromatin without ligation and non-crosslinked genomic DNA with or without ligation were used as negative controls. The bands shown in the bottom panels of B and C represented the PCR products from genomic DNA that was not cut by any restriction enzyme, which were used as the loading control.
    Asei, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 150 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/asei/product/New England Biolabs
    Average 93 stars, based on 150 article reviews
    Price from $9.99 to $1999.99
    asei - by Bioz Stars, 2022-10
    93/100 stars

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    1) Product Images from "The BCL2 gene is regulated by a special AT-rich sequence binding protein 1-mediated long range chromosomal interaction between the promoter and the distal element located within the 3?-UTR"

    Article Title: The BCL2 gene is regulated by a special AT-rich sequence binding protein 1-mediated long range chromosomal interaction between the promoter and the distal element located within the 3?-UTR

    Journal: Nucleic Acids Research

    doi: 10.1093/nar/gkr023

    3C Analysis of the interaction between the mbr and the BCL2 promoter region in vivo . The scheme of BCL2 gene with the positions of restriction sites and positions of primers was indicated ( A ). The 3C experiments were performed as described in the ‘Materials and Methods’ section. Briefly, formaldehyde was used to crosslink protein–DNA interactions in intact nuclei. The crosslinked chromatin was then digested by AseI ( B ) or HindIII ( C ), followed by ligation. Physical interaction between mbr and SBS1 or SBS2 was then determined by specific PCRs that detected hybrid fragments containing either mbr/SBS1 sequences or mbr/SBS2 sequences. 3C data demonstrated that the mbr specifically interacted with SBS1 (B, up panel), but not with SBS2 (C, up panel). PCR products from AseI (B) or HindIII (C) digested crosslinked chromatin without ligation and non-crosslinked genomic DNA with or without ligation were used as negative controls. The bands shown in the bottom panels of B and C represented the PCR products from genomic DNA that was not cut by any restriction enzyme, which were used as the loading control.
    Figure Legend Snippet: 3C Analysis of the interaction between the mbr and the BCL2 promoter region in vivo . The scheme of BCL2 gene with the positions of restriction sites and positions of primers was indicated ( A ). The 3C experiments were performed as described in the ‘Materials and Methods’ section. Briefly, formaldehyde was used to crosslink protein–DNA interactions in intact nuclei. The crosslinked chromatin was then digested by AseI ( B ) or HindIII ( C ), followed by ligation. Physical interaction between mbr and SBS1 or SBS2 was then determined by specific PCRs that detected hybrid fragments containing either mbr/SBS1 sequences or mbr/SBS2 sequences. 3C data demonstrated that the mbr specifically interacted with SBS1 (B, up panel), but not with SBS2 (C, up panel). PCR products from AseI (B) or HindIII (C) digested crosslinked chromatin without ligation and non-crosslinked genomic DNA with or without ligation were used as negative controls. The bands shown in the bottom panels of B and C represented the PCR products from genomic DNA that was not cut by any restriction enzyme, which were used as the loading control.

    Techniques Used: In Vivo, Ligation, Polymerase Chain Reaction

    2) Product Images from "High Frequency of Cryptosporidium hominis Infecting Infants Points to A Potential Anthroponotic Transmission in Maputo, Mozambique"

    Article Title: High Frequency of Cryptosporidium hominis Infecting Infants Points to A Potential Anthroponotic Transmission in Maputo, Mozambique

    Journal: Pathogens

    doi: 10.3390/pathogens10030293

    Genotyping of the Cryptosporidium parasites by PCR-RFLP targeting 18S rRNA gene. M, molecular size makers (100 bp). Lane 1: C. parvum ; Lanes 2, 3 and 4: C. hominis and Lane 5: mixed infection with C. hominis and C. parvum . The upper lanes show SspI digestion products showing a molecular size from 111 bp to 449 bp, and the lower lanes show AseI digestion products with molecular size of approximately 104 bp to 628 bp.
    Figure Legend Snippet: Genotyping of the Cryptosporidium parasites by PCR-RFLP targeting 18S rRNA gene. M, molecular size makers (100 bp). Lane 1: C. parvum ; Lanes 2, 3 and 4: C. hominis and Lane 5: mixed infection with C. hominis and C. parvum . The upper lanes show SspI digestion products showing a molecular size from 111 bp to 449 bp, and the lower lanes show AseI digestion products with molecular size of approximately 104 bp to 628 bp.

    Techniques Used: Polymerase Chain Reaction, Infection

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    New England Biolabs asei
    3C Analysis of the interaction between the mbr and the BCL2 promoter region in vivo . The scheme of BCL2 gene with the positions of restriction sites and positions of primers was indicated ( A ). The 3C experiments were performed as described in the ‘Materials and Methods’ section. Briefly, formaldehyde was used to crosslink protein–DNA interactions in intact nuclei. The crosslinked chromatin was then digested by <t>AseI</t> ( B ) or HindIII ( C ), followed by ligation. Physical interaction between mbr and <t>SBS1</t> or SBS2 was then determined by specific PCRs that detected hybrid fragments containing either mbr/SBS1 sequences or mbr/SBS2 sequences. 3C data demonstrated that the mbr specifically interacted with SBS1 (B, up panel), but not with SBS2 (C, up panel). PCR products from AseI (B) or HindIII (C) digested crosslinked chromatin without ligation and non-crosslinked genomic DNA with or without ligation were used as negative controls. The bands shown in the bottom panels of B and C represented the PCR products from genomic DNA that was not cut by any restriction enzyme, which were used as the loading control.
    Asei, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/asei/product/New England Biolabs
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    asei - by Bioz Stars, 2022-10
    93/100 stars
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    3C Analysis of the interaction between the mbr and the BCL2 promoter region in vivo . The scheme of BCL2 gene with the positions of restriction sites and positions of primers was indicated ( A ). The 3C experiments were performed as described in the ‘Materials and Methods’ section. Briefly, formaldehyde was used to crosslink protein–DNA interactions in intact nuclei. The crosslinked chromatin was then digested by AseI ( B ) or HindIII ( C ), followed by ligation. Physical interaction between mbr and SBS1 or SBS2 was then determined by specific PCRs that detected hybrid fragments containing either mbr/SBS1 sequences or mbr/SBS2 sequences. 3C data demonstrated that the mbr specifically interacted with SBS1 (B, up panel), but not with SBS2 (C, up panel). PCR products from AseI (B) or HindIII (C) digested crosslinked chromatin without ligation and non-crosslinked genomic DNA with or without ligation were used as negative controls. The bands shown in the bottom panels of B and C represented the PCR products from genomic DNA that was not cut by any restriction enzyme, which were used as the loading control.

    Journal: Nucleic Acids Research

    Article Title: The BCL2 gene is regulated by a special AT-rich sequence binding protein 1-mediated long range chromosomal interaction between the promoter and the distal element located within the 3?-UTR

    doi: 10.1093/nar/gkr023

    Figure Lengend Snippet: 3C Analysis of the interaction between the mbr and the BCL2 promoter region in vivo . The scheme of BCL2 gene with the positions of restriction sites and positions of primers was indicated ( A ). The 3C experiments were performed as described in the ‘Materials and Methods’ section. Briefly, formaldehyde was used to crosslink protein–DNA interactions in intact nuclei. The crosslinked chromatin was then digested by AseI ( B ) or HindIII ( C ), followed by ligation. Physical interaction between mbr and SBS1 or SBS2 was then determined by specific PCRs that detected hybrid fragments containing either mbr/SBS1 sequences or mbr/SBS2 sequences. 3C data demonstrated that the mbr specifically interacted with SBS1 (B, up panel), but not with SBS2 (C, up panel). PCR products from AseI (B) or HindIII (C) digested crosslinked chromatin without ligation and non-crosslinked genomic DNA with or without ligation were used as negative controls. The bands shown in the bottom panels of B and C represented the PCR products from genomic DNA that was not cut by any restriction enzyme, which were used as the loading control.

    Article Snippet: Hundred units of the restriction enzyme (NEB) (AseI for SBS1-mbr, HindIII for SBS2/mbr) were added for a 22-h digestion.

    Techniques: In Vivo, Ligation, Polymerase Chain Reaction

    Restriction enzyme digestion pattern of nine bacteriophages. Genomic DNA from bacteriophages are extracted and digested with Ase I for all phages and with Hind III for ΦFifi044 and ΦFifi451 for 2 h. (A) Agarose gel electrophoresis restriction patterns of DNA digested with Ase I. Lane M1, 1 kb plus ladder (Dyne Bio Inc., Seongnam, Korea); lane 1, ΦFifi011; lane 2, ΦFifi044; lane 3, ΦFifi051; lane 4, ΦFifi067; lane 5, ΦFifi106; lane 6, ΦFifi287; lane 7, ΦFifi318; lane 8, ΦFifi450; lane 9, ΦFifi451; lane M2, Lambda/ Hind III marker (Bioneer, Daejeon, Korea). (B) Agarose gel electrophoresis restriction patterns of DNA digested with Hind III. Lane M1, 1 kb plus ladder; lane 2, ΦFifi044; lane 9, ΦFifi451; lane M2, Lambda/ Hind III marker.

    Journal: The Plant Pathology Journal

    Article Title: Isolation of Nine Bacteriophages Shown Effective against Erwinia amylovora in Korea

    doi: 10.5423/PPJ.NT.11.2021.0172

    Figure Lengend Snippet: Restriction enzyme digestion pattern of nine bacteriophages. Genomic DNA from bacteriophages are extracted and digested with Ase I for all phages and with Hind III for ΦFifi044 and ΦFifi451 for 2 h. (A) Agarose gel electrophoresis restriction patterns of DNA digested with Ase I. Lane M1, 1 kb plus ladder (Dyne Bio Inc., Seongnam, Korea); lane 1, ΦFifi011; lane 2, ΦFifi044; lane 3, ΦFifi051; lane 4, ΦFifi067; lane 5, ΦFifi106; lane 6, ΦFifi287; lane 7, ΦFifi318; lane 8, ΦFifi450; lane 9, ΦFifi451; lane M2, Lambda/ Hind III marker (Bioneer, Daejeon, Korea). (B) Agarose gel electrophoresis restriction patterns of DNA digested with Hind III. Lane M1, 1 kb plus ladder; lane 2, ΦFifi044; lane 9, ΦFifi451; lane M2, Lambda/ Hind III marker.

    Article Snippet: One μg genomic DNA was digested with restriction enzyme Ase I (NEB, Ipswich, MA, USA) or Hind III (Takara, Tokyo, Japan) in a final volume of 20 μl at 37°C for 2 h. Two μl of LoadingSTAR (Dyne Bio Inc., Seongnam, Korea) was mixed with 10 μl of samples and loaded on an 0.7% agarose gel (70 V, 2 h).

    Techniques: Agarose Gel Electrophoresis, Marker

    Genotyping of the Cryptosporidium parasites by PCR-RFLP targeting 18S rRNA gene. M, molecular size makers (100 bp). Lane 1: C. parvum ; Lanes 2, 3 and 4: C. hominis and Lane 5: mixed infection with C. hominis and C. parvum . The upper lanes show SspI digestion products showing a molecular size from 111 bp to 449 bp, and the lower lanes show AseI digestion products with molecular size of approximately 104 bp to 628 bp.

    Journal: Pathogens

    Article Title: High Frequency of Cryptosporidium hominis Infecting Infants Points to A Potential Anthroponotic Transmission in Maputo, Mozambique

    doi: 10.3390/pathogens10030293

    Figure Lengend Snippet: Genotyping of the Cryptosporidium parasites by PCR-RFLP targeting 18S rRNA gene. M, molecular size makers (100 bp). Lane 1: C. parvum ; Lanes 2, 3 and 4: C. hominis and Lane 5: mixed infection with C. hominis and C. parvum . The upper lanes show SspI digestion products showing a molecular size from 111 bp to 449 bp, and the lower lanes show AseI digestion products with molecular size of approximately 104 bp to 628 bp.

    Article Snippet: Genotype identification was made through the analysis of pattern of the secondary product after restriction digestion with the enzymes SspI and AseI (New England BioLabs Inc., Beverly, MA, USA) as described by Xiao et al. [ ].

    Techniques: Polymerase Chain Reaction, Infection